Genomes and Genes
Gene Symbol: SLC25A15
Description: solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15
Alias: D13S327, HHH, ORC1, ORNT1, mitochondrial ornithine transporter 1, ornithine transporter 1, solute carrier family 25 member 15
Publications124 found, 100 shown here
- Essential and non-essential DNA replication genes in the model halophilic Archaeon, Halobacterium sp. NRC-1Brian R Berquist
University of Maryland Biotechnology Institute, Center of Marine Biotechnology, Baltimore, Maryland 21202, USA
BMC Genet 8:31. 2007..We have developed a tractable genetic system for knockout analysis of genes in the model halophilic archaeon, Halobacterium sp. NRC-1, and used it to determine which DNA replication genes are essential...
- The human and mouse SLC25A29 mitochondrial transporters rescue the deficient ornithine metabolism in fibroblasts of patients with the hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndromeJosé A Camacho
Department of Pediatrics, University of California Irvine, Irvine, CA 92697, USA
Pediatr Res 66:35-41. 2009..urea cycle (UCD) and ornithine degradation pathway caused by mutations in the mitochondrial ornithine transporter (ORNT1)...
- Cloning and characterization of human ORNT2: a second mitochondrial ornithine transporter that can rescue a defective ORNT1 in patients with the hyperornithinemia-hyperammonemia-homocitrullinuria syndrome, a urea cycle disorderJosé A Camacho
Department of Pediatrics, University of Oklahoma Health Sciences Center, 975 N E 10th Street, Biomedical Research Center, Room BRC 256, Oklahoma City, OK 73104, USA
Mol Genet Metab 79:257-71. 2003We recently characterized the mitochondrial ornithine transporter (ORNT1), the gene defective in the hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome, a urea cycle disorder...
- Crystal structures of human DNA polymerase beta complexed with DNA: implications for catalytic mechanism, processivity, and fidelityH Pelletier
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla 92093 0506, USA
Biochemistry 35:12742-61. 1996..pocket that accommodates a 5'-PO4 end group, the 8-kDa domain also contains a newly discovered helix-hairpin-helix (HhH) motif that binds to DNA in the same way as does a structurally and sequentially homologous HhH motif in the 31-kDa ..
- A genetic screen for replication initiation defective (rid) mutants in Schizosaccharomyces pombeAlexandra M Locovei
Department of Molecular and Cellular Phamacology, University of Miami School of Medicine PO Box 016189, Miami, FL 33140, USA
Cell Div 5:20. 2010..Our screen identified two mutants, rid1-1 and rid2-1, that are defective in Orc1 and Mcm4, respectively...
- Genetic and physical mapping of DNA replication origins in Haloferax volcaniiCedric Norais
Institut de Genetique et Microbiologie, Universite Paris Sud, Orsay, France
PLoS Genet 3:e77. 2007..Genes for the initiator protein Cdc6/Orc1, which are commonly located adjacent to archaeal origins of DNA replication, are found on all replicons except ..
- Genetic variation in the urea cycle: a model resource for investigating key candidate genes for common diseasesSabrina Mitchell
Center for Human Genetics Research, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
Hum Mutat 30:56-60. 2009..boundaries of the five key urea cycle enzymes, NAGS, and two solute carrier transporter genes (SLC25A13 and SLC25A15) for sequence alterations using single-stranded conformational polymorphism (SSCP) analysis and high-resolution ..
- Genomewide and biochemical analyses of DNA-binding activity of Cdc6/Orc1 and Mcm proteins in Pyrococcus spFujihiko Matsunaga
Institut de Genetique et Microbiologie, UMR8621, Bat 409, Universite Paris Sud, 91405 Orsay Cedex, France
Nucleic Acids Res 35:3214-22. 2007..We report here that the binding of Cdc6/Orc1 to a 5 kb region containing oriC in vivo was highly specific both in exponential and stationary phases, by means of ..
- RNA interference targeting ORC1 gene suppresses the proliferation of vascular smooth muscle cells in ratsMao Qin Shu
Department of Cardiology, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China
Exp Mol Pathol 84:206-12. 2008..The largest subunit of the origin recognition complex (ORC), ORC1, plays a critical role during the initiation of DNA replication in eukaryotes...
- Hyperornithinemia-hyperammonemia-homocitrullinuria syndrome (HHH) presenting with acute fulminant hepatic failureA A Mhanni
Department of Pediatrics and Child Health, University of Manitoba, Canada
J Pediatr Gastroenterol Nutr 46:312-5. 2008..Molecular analysis of the SLC25A15 gene showed that the two patients were homozygous for the common French Canadian mutation (F188Delta)...
- Octahedral tilting in Pb-based relaxor ferroelectrics at high pressureBernd J Maier
Department Geowissenschaften, , Grindelallee 48, 20146 Hamburg, Germany
Acta Crystallogr B 66:280-91. 2010..Synchrotron single-crystal X-ray diffraction data show that the intensity of hhh peaks, h = 2n + 1, does not change with pressure...
- Functional changes in a novel uracil-DNA glycosylase determined by mutational analysesE K Im
Yonsei Research Institute of Aging Science, Yonsei University, Seoul 120 749, South Korea
Mikrobiologiia 77:644-50. 2008..Methanococcus jannaschii UDG (MjUDG), a novel monofunctional glycosylase, contains a helix-hairpin-helix (HhH) motif and Gly/Pro rich loop (GPD region), which is important for catalytic activity; it shares these features with ..
- Mammary sensitivity to protein restriction and re-alimentationM G Goodwill
Institute of Ecology and Resource Management, University of Edinburgh
Br J Nutr 76:423-34. 1996..Lactational dietary treatments were the supply ad libitum of either diet H (HHH) or diet L (LLL) for the first 12 d of lactation, or diet L transferring to diet H on either day 6 (LHH) or 9 (LLH) ..
- Novel adhesin from Pasteurella multocida that binds to the integrin-binding fibronectin FnIII9-10 repeatsLisa M Mullen
Division of Microbial Diseases, Eastman Dental Institute, University College London, 256 Gray s Inn Road, London WC1X 8LD, United Kingdom
Infect Immun 76:1093-104. 2008..Bioinformatics analysis suggests that the PM1665 protein contains two helix-hairpin-helix (HhH) motifs, and truncation mutation studies have identified the binding site in the protein as a combination of these ..
- Crystal structure of an archaeal Ski2p-like protein from Pyrococcus horikoshii OT3Xiaodong Zhang
Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University, Fukuoka 812 8581, Japan
Protein Sci 17:136-45. 2008..C1 and C2) fold into alpha-helical structures containing a winged helix (WH)-fold and helix-hairpin-helix (HhH)-fold, respectively...
- Intra-arterial verapamil-induced seizures: case report and review of the literatureFranklin D Westhout
Department of Neurological Surgery, University of California Irvine School of Medicine, Orange, CA 92868, USA
Surg Neurol 67:483-6; discussion 486. 2007..Seizures occurring from IA infusion of verapamil are rare...
- Structure and activity of a thermostable thymine-DNA glycosylase: evidence for base twisting to remove mismatched normal DNA basesClifford D Mol
Department of Molecular Biology MB4, Skaggs Institute for Chemical Biology, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
J Mol Biol 315:373-84. 2002..repair can be initiated by a specific mismatch glycosylase (MIG) that is homologous to the helix-hairpin-helix (HhH) DNA repair enzymes. Here, we present a 2...
- Clinical and functional characterization of a human ORNT1 mutation (T32R) in the hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndromeJosé A Camacho
Department of Pediatrics, Division of Human Genetics, University of California, Irvine, CA 92697 3940, USA
Pediatr Res 60:423-9. 2006..a disorder of the urea cycle and ornithine degradation pathway, who have the same novel ornithine transporter (ORNT1) genotype (T32R) but a variable phenotype...
- Orotic acid quantification in dried blood spots and biological fluids by hydrophilic interaction liquid chromatography tandem mass spectrometryOceania D'Apolito
Department of Biomedical Sciences, University of Foggia, Foggia, Italy
J Sep Sci 33:966-73. 2010..A patient with hyperammonemia-hyperornithinemia-homocitrullinemia (HHH) syndrome presented a urinary ORA level of 9.1 mmol/mol of creatinine...
- The HhH2/NDD domain of the Drosophila Nod chromokinesin-like protein is required for binding to chromosomes in the oocyte nucleusWei Cui
Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO 64110, USA
Genetics 171:1823-35. 2005..The second putative DNA-binding domain, denoted HhH(2)/NDD, is a helix-hairpin-helix(2)/Nod-like DNA-binding domain...
- DNA replication and the GINS complex: localization on extended chromatin fibersStephanie M Cohen
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
Epigenetics Chromatin 2:6. 2009..RESULTS: Using immunofluorescence microscopy we were able to visualize ORC1, ORC2, PCNA, and GINS complex proteins Psf1 and Psf2 bound to extended chromatin fibers...
- Trypanosome prereplication machinery contains a single functional orc1/cdc6 protein, which is typical of archaeaPatrícia Diogo de Melo Godoy
Laboratorio de Parasitologia, Instituto Butantan, Av Vital Brasil, 1500, São Paulo 05503900, Brazil
Eukaryot Cell 8:1592-603. 2009..the replication origin is recognized by the heterohexamer origin recognition complex (ORC) containing six proteins, Orc1 to Orc6, while in members of the domain Archaea, the replication origin is recognized by just one protein, Orc1/..
- HHH regime for arteritis secondary to TB meningitis: a prospective randomized studyArunodaya R Gujjar
Department of Neurology, National Institute of Mental Health and Neurosciences, Bangalore, India
Neurocrit Care 10:313-7. 2009..Few treatment regimes specifically address this condition. Hypervolemia-hypertension-hemodilution (HHH) regime is known to be effective for treatment of vasospasm complicating subarachnoid hemorrhage...
- Mutational studies of Pa-AGOG DNA glycosylase from the hyperthermophilic crenarchaeon Pyrobaculum aerophilumGondichatnahalli M Lingaraju
Biomolecular Research, Paul Scherrer Institut, CH 5232 Villigen, Switzerland
DNA Repair (Amst) 8:857-64. 2009..glycosylase, Pa-AGOG (Archaeal GO DNA glycosylase) which has become the founding member of a new family within the HhH-GPD superfamily of DNA glycosylases based on unique structural and functional characteristics...
- The cyclin A centrosomal localization sequence recruits MCM5 and Orc1 to regulate centrosome reduplicationRebecca L Ferguson
Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA
J Cell Sci 123:2743-9. 2010..Previous studies have demonstrated a role for the DNA replication factors MCM5 and Orc1 in preventing centrosome reduplication...
- Conscientious metabolic monitoring on a patient with hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome undergoing anaesthesiaJ Muhling
Department of Anaesthesiology and Intensive Care Medicine, Justus Liebig University Giessen, Federal Republic of Germany
Amino Acids 21:303-18. 2001..combination of increased plasma ornithine concentrations, postprandial hyperammonemia, and homocitrullinuria (HHH-syndrome)...
- Binding of AlF-C, an Orc1-binding transcriptional regulator, enhances replicator activity of the rat aldolase B originHiroyuki Minami
Cryobiosystem Research Center, Iwate University, Ueda, Morioka, Iwate 020 8550, Japan
Mol Cell Biol 26:8770-80. 2006..Mutation of site C in the rat origin, which binds an Orc1-binding protein AlF-C in vitro, resulted in a significant reduction of the replicator activity in the mouse cells...
- Cdc6/Orc1 from Pyrococcus furiosus may act as the origin recognition protein and Mcm helicase recruiterMasaki Akita
Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6 10 1 Hakozaki, Higashi ku, Fukuoka, Fukuoka 812 8581, Japan
Genes Cells 15:537-52. 2010Archaea have one or more Cdc6/Orc1 proteins, which share sequence similarities with eukaryotic Cdc6 and Orc1...
- Safety of intrathecal sodium nitroprusside for the treatment and prevention of refractory cerebral vasospasm and ischemia in humansJ E Thomas
Department of Neurological Surgery, Division of Cerebrovascular Surgery, Thomas Jefferson University, Wills Eye Hospital, Philadelphia, PA, USA
Stroke 30:1409-16. 1999..The mechanism of DCV remains unknown. A new treatment for refractory DCV using intrathecally delivered sodium nitroprusside and results in 21 patients is reported...
- Health consequences of Alzheimer's caregiving transitions: effects of placement and bereavementIgor Grant
Department of Psychiatry, University of California, San Diego, La Jolla, California 92093 0680, USA
Psychosom Med 64:477-86. 2002....
- Is hereditary hemochromatosis a balanced polymorphism: an analysis of family size among hemochromatosis heterozygotesR L Nelson
Department of Surgery, College of Medicine, University of Illinois at Chicago, 1740 West Taylor Street, Room 2204, m c 957, Chicago, Illinois 60612, USA
Hepatogastroenterology 48:523-6. 2001..Evidence for this was sought by assessing reproductive success among hemochromatosis heterozygotes through determining their family size and incidence of miscarriage when compared to nongene carriers...
- The archaeal cell cycle: current issuesRolf Bernander
Department of Molecular Evolution, Evolutionary Biology Center, Uppsala University, Norbyvagen 18C, SE 752 36 Uppsala, Sweden
Mol Microbiol 48:599-604. 2003The recently discovered structural similarities between the archaeal Orc1/Cdc6 and bacterial DnaA initiator proteins for chromosome replication have exciting implications for cell cycle regulation...
- Localized melting of duplex DNA by Cdc6/Orc1 at the DNA replication origin in the hyperthermophilic archaeon Pyrococcus furiosusFujihiko Matsunaga
Department of Genetic Resources Technology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6 10 1 Hakozaki, Higashi ku, Fukuoka, Fukuoka 812 8581, Japan
Extremophiles 14:21-31. 2010..Although recent studies in Archaea defined the origin of DNA replication (oriC) and the Cdc6/Orc1 homolog as an origin recognition protein, the location and mechanism of duplex opening have remained unclear...
- Pa-AGOG, the founding member of a new family of archaeal 8-oxoguanine DNA-glycosylasesAlessandro A Sartori
Institute of Molecular Cancer Research, University of Zurich, August Forel Strasse 7, Ch 8008 Zurich, Switzerland
Nucleic Acids Res 32:6531-9. 2004..a helix-hairpin-helix motif and a glycine/proline-rich sequence followed by an absolutely conserved aspartate (HhH-GPD motif). Interestingly, although the P...
- Chromosome replication dynamics in the archaeon Sulfolobus acidocaldariusIain G Duggin
Medical Research Council Cancer Cell Unit, Hutchison Medical Research Council Research Centre, Hills Road, Cambridge, CB2 0XZ, United Kingdom
Proc Natl Acad Sci U S A 105:16737-42. 2008..Analysis of the 3 Orc1/Cdc6 initiator proteins showed a uniformity of cellular abundance and origin binding throughout the cell cycle...
- A DNA glycosylase from Pyrobaculum aerophilum with an 8-oxoguanine binding mode and a noncanonical helix-hairpin-helix structureGondichatnahalli M Lingaraju
Biomolecular Research, Paul Scherrer Institut, CH 5232 Villigen, Switzerland
Structure 13:87-98. 2005..are identified that confirm Pa-AGOG to be the founding member of a functional class within the helix-hairpin-helix (HhH) superfamily of DNA repair enzymes...
- Elaboration, diversification and regulation of the Sir1 family of silencing proteins in SaccharomycesJennifer E G Gallagher
Department of Molecular and Cell Biology, California Institute of Quantitative Biosciences, University of California, Berkeley, CA 94720 3220, USA
Genetics 181:1477-91. 2009..at HML and HMR via its ORC interacting region (OIR), which binds the bromo adjacent homology (BAH) domain of Orc1. Zygosaccharomyces rouxii, which diverged from Saccharomyces after the appearance of the silent mating cassettes, ..
- Proteomic analysis of human O6-methylguanine-DNA methyltransferase by affinity chromatography and tandem mass spectrometrySuryakant K Niture
Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX 79106, USA
Biochem Biophys Res Commun 337:1176-84. 2005..proteins with diverse functions including those involved in DNA replication and repair (MCM2, PCNA, ORC1, DNA polymerase delta, MSH-2, and DNA-dependent protein kinase), cell cycle progression (CDK1, cyclin B, CDK2, CDC7,..
- Deoxyribose phosphate excision by the N-terminal domain of the polymerase beta: the mechanism revisitedJ A Feng
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
Biochemistry 37:9605-11. 1998..This mechanism focuses on a catalytic groove near the helix-hairpin-helix (HhH) motif of the 8 kDa domain...
- Hormonal induction of hepatic mitochondrial ornithine/citrulline transporter mRNASidney M Morris
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, W1255 Biomedical Science Tower, PA 15206, USA
Biochem Biophys Res Commun 294:749-52. 2002..transport of ornithine and citrulline across the mitochondrial membrane by the ornithine/citrulline antiporter ORNT1. Expression of the urea cycle enzymes can change dramatically in response to hormones, but it is not known whether ..
- Over-expression of human DNA polymerase lambda in E. coli and characterization of the recombinant enzymeNoriko Shimazaki
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Noda, Chiba 278 8510, Japan
Genes Cells 7:639-51. 2002..a nuclear localization signal (NLS), a BRCA1-C terminal (BRCT) domain, a proline-rich region, helix-hairpin-helix (HhH) and pol X motifs...
- Transcriptional silencing functions of the yeast protein Orc1/Sir3 subfunctionalized after gene duplicationMeleah A Hickman
Biochemistry Department, Institute for Genome Sciences and Policy, Program in Genetics and Genomics, Duke University, Durham, NC 27708, USA
Proc Natl Acad Sci U S A 107:19384-9. 2010..The largest subunit of ORC, Orc1, is particularly interesting because it contains a nucleosome-binding BAH domain and because it gave rise to Sir3, ..
- Characterization of the metal ion binding helix-hairpin-helix motifs in human DNA polymerase beta by X-ray structural analysisH Pelletier
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla 92093 0506, USA
Biochemistry 35:12778-87. 1996..beta (pol beta) occurs primarily through two structurally and sequentially homologous helix-hairpin-helix (HhH) motifs, one in the fingers subdomain and the other in the 8-kDa domain [Pelletier, H., Sawaya, M. R., Wolfle, W...
- Conformational changes induced by nucleotide binding in Cdc6/ORC from Aeropyrum pernixMartin R Singleton
Cancer Research UK Clare Hall Laboratories, The London Research Institute, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3LD, UK
J Mol Biol 343:547-57. 2004..suggests the existence of at least two subfamilies of these proteins, for which we propose the nomenclature ORC1 and ORC2...
- The essential role of Saccharomyces cerevisiae CDC6 nucleotide-binding site in cell growth, DNA synthesis, and Orc1 associationB Wang
Department of Pediatrics, University of Southern California, Los Angeles, California 90027, USA
J Biol Chem 274:8291-8. 1999..the molecular mechanism of nucleotide-binding function, we have demonstrated that the Cdc6 protein associates with Orc1 in vitro and in vivo...
- Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammalsD A Natale
National Institute of Child Health and Human Development, Building 6, Room 3A02, National Institutes of Health, Bethesda, MD 20892 2753, USA
EMBO J 19:2728-38. 2000..the time when hamster origin recognition complexes (ORCs) became functional was related to the time when Orc1, Orc2 and Mcm3 proteins became stably bound to hamster chromatin...
- HLA-A2.1-restricted education and cytolytic activity of CD8(+) T lymphocytes from beta2 microglobulin (beta2m) HLA-A2.1 monochain transgenic H-2Db beta2m double knockout miceS Pascolo
Institut Pasteur, Département SIDA Rétrovirus, Unité d Immunité Cellulaire Antivirale, 75724 Paris Cedex 15, France
J Exp Med 185:2043-51. 1997..beta2m) is covalently linked to the NH2 terminus of the heavy chain by a 15- amino acid long peptide: HHH, entirely human, HHD, with the mouse H-2Db alpha3, transmembrane, and cytoplasmic domains, and MHD, homologous to ..
- Functional differentiation and cooperative interaction between two eukaryote-like archaeal Orc1/Cdc6 proteins on the replication originPei Xia Jiang
Center for Proteomics Research, National Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Biochem Biophys Res Commun 364:945-51. 2007The DNA replication apparatus of archaea represents a core version of that in eukaryotes. Archaeal Orc1/Cdc6s can be an integral component in the replication machineries cooperatively regulating DNA replication...
- Novel splicing variant of mouse Orc1 is deficient in nuclear translocation and resistant for proteasome-mediated degradationYasuyuki Miyake
Cellular Physiology Laboratory, RIKEN Discovery Research Institute, Wako, Saitama 351 0198, Japan
J Biol Chem 280:12643-52. 2005..of the mammalian ORC, we cloned ORC subunit cDNAs from mouse NIH3T3 cells and found novel variant forms of Orc1, Orc2, and Orc3 each derived from alternative RNA splicing...
- Structure-guided mutational analysis of the OB, HhH, and BRCT domains of Escherichia coli DNA ligaseLi Kai Wang
Molecular Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
J Biol Chem 283:23343-52. 2008..and oligonucleotide-binding (OB) domains is linked via a tetracysteine zinc finger to distal helix-hairpin-helix (HhH) and BRCT (BRCA1-like C-terminal) domains...
- Creatine metabolism in urea cycle defectsSara Boenzi
Division of Metabolism and Research Unit of Metabolic Biochemistry, Bambino Gesu Children s Hospital, IRCCS, Piazza S Onofrio 4, 00165, Rome, Italy
J Inherit Metab Dis 35:647-53. 2012..e., OTC, ASS, ASL deficiencies, HHH syndrome and lysinuric protein intolerance)...
- Long-term follow-up of four patients affected by HHH syndromeSook Z Kim
Korea Genetic Research Center, Cheong Ju, Heung Deok Gu, Republic of Korea
Clin Chim Acta 413:1151-5. 2012In hyperornithinemia-hyperammonemia-homocitrullinemia (HHH) syndrome, impaired ornithine transport across the mitochondrial membrane causes ornithine accumulation in cytoplasm...
- Cdc6-induced conformational changes in ORC bound to origin DNA revealed by cryo-electron microscopyJingchuan Sun
Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Structure 20:534-44. 2012..The six subunits of ORC are arranged as Orc1:Orc4:Orc5:Orc2:Orc3, with Orc6 binding to Orc2...
- Insights into the mutation-induced HHH syndrome from modeling human mitochondrial ornithine transporter-1Jing Fang Wang
Key Laboratory of Systems Biomedicine, Ministry of Education, Shanghai Jiao Tong University, Shanghai, China
PLoS ONE 7:e31048. 2012..ornithine transporter-1 is reported in coupling with the hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome, which is a rare autosomal recessive disorder...
- Substrate specificity of the two mitochondrial ornithine carriers can be swapped by single mutation in substrate binding siteMagnus Monné
Department of Pharmaco Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, Via E Orabona 4, 70125 Bari, Italy
J Biol Chem 287:7925-34. 2012..Here we have characterized mutations of the proposed substrate binding site in the human ornithine carriers ORC1 and ORC2 by carrying out transport assays with a set of different substrates...
- Dual mechanism of brain damage induced in vivo by the major metabolites accumulating in hyperornithinemia-hyperammonemia-homocitrullinuria syndromeCarolina Maso Viegas
Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, UFRGS, Porto Alegre, RS, Brazil
Brain Res 1369:235-44. 2011Hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome is an autosomal recessive disorder caused by a defect in the mitochondrial ornithine transporter, leading to accumulation of ornithine (Orn), homocitrulline (Hcit) and ..
- Experimental evidence that ornithine and homocitrulline disrupt energy metabolism in brain of young ratsCarolina Maso Viegas
Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, UFRGS, Porto Alegre, RS, Brazil
Brain Res 1291:102-12. 2009..acid (Oro) is the biochemical hallmark of patients affected by hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome, a disorder clinically characterized by neurological symptoms, whose pathophysiology is practically ..
- Expression of the HsOrc1 gene, a human ORC1 homolog, is regulated by cell proliferation via the E2F transcription factorK Ohtani
Department of Genetics, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
Mol Cell Biol 16:6977-84. 1996..Expression of the human Orc1 gene (HsOrc1) is low in quiescent cells, and it is then dramatically induced upon stimulation of cell growth...
- Assessing individual bioequivalence with high-order cross-over designs: a unified procedureFrancis C Hsuan
Temple University, Philadelphia, PA 19122, USA
Stat Med 22:2847-60. 2003..The recommended approach to the analysis is to follow the methodology of Hyslop, Hsuan and Holder (HHH), based on a linear mixed model...
- Non-specific DNA binding interferes with the efficient excision of oxidative lesions from chromatin by the human DNA glycosylase, NEIL1Ian D Odell
Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, United States
DNA Repair (Amst) 9:134-43. 2010..We reported recently that the human DNA glycosylase hNTH1 (human Endonuclease III), a member of the HhH GpG superfamily of glycosylases, can excise thymine glycol lesions from nucleosomes without requiring or inducing ..
- Quantitation of orotic acid in urine using isotope dilution-selected ion gas chromatography-mass spectrometryJie Chen
Department of Pathology and Laboratory Medicine, The Children s Hospital of Philadelphia, Philadelphia, PA, USA
Methods Mol Biol 603:445-51. 2010..including ornithine transcarbamylase (OTC) deficiency, and the hyperornithinemia-hyperammonemia-homocitrullinemia (HHH) syndrome. Low levels of orotic acid are observed in carbamylphosphate synthetase (CPS) defects...
- An evolutionary analysis of the helix-hairpin-helix superfamily of DNA repair glycosylasesDee R Denver
Department of Biology, Indiana University, USA
Mol Biol Evol 20:1603-11. 2003The helix-hairpin-helix (HhH) superfamily of base excision repair DNA glycosylases is composed of multiple phylogenetically diverse enzymes that are capable of excising varying spectra of oxidatively and methyl-damaged bases...
- Novel insights into the plant histone code: lessons from ORC1María de la Paz Sanchez
Centro de Biologia Molecular Severo Ochoa, CSIC UAM, Cantoblanco, Madrid 28049, Spain
Epigenetics 4:205-8. 2009..example that illustrates such diversity of the histone code is the large subunit of the origin recognition complex, ORC1. In plants, but not in yeast and animal cells, ORC1 functions as a transcriptional activator of a subset of target ..
- The mitochondrial transporter family (SLC25): physiological and pathological implicationsFerdinando Palmieri
Department of Pharmaco Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, Via Orabona 4, 70125, Bari, Italy
Pflugers Arch 447:689-709. 2004..g. hyperornithinaemia, hyperammonaemia and homocitrullinuria (HHH) syndrome and type II citrullinemia...
- Archaeal eukaryote-like Orc1/Cdc6 initiators physically interact with DNA polymerase B1 and regulate its functionsLu Zhang
National Key Laboratory of Agricultural Microbiology, Center for Proteomics Research, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Proc Natl Acad Sci U S A 106:7792-7. 2009..In the current study, the Orc1/Cdc6 (SsoCdc6) proteins from the crenarchaeon Sulfolobus solfataricus were found to physically interact with its ..
- Human CDC6/Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phaseP Saha
Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Cell Biol 18:2758-67. 1998..with human PCNA, we identified and cloned a human protein (hCdc18) homologous to yeast CDC6/Cdc18 and human Orc1. Unlike yeast, in which the rapid and total destruction of CDC6/Cdc18 protein in S phase is a central feature of ..
- Proteins of the origin recognition complex (ORC) and DNA topoisomerases on mammalian chromatinHong Gang Hu
Department of Biology, University of Konstanz, Konstanz, Germany
BMC Mol Biol 10:36. 2009..In order to provide more information about topoisomerases in the initiation of mammalian replication, we investigated whether topoisomerases occur close to ORC in the chromatin of cultured human HeLa cells...
- The origin recognition complex protein familyBernard P Duncker
Department of Biology, University of Waterloo, Waterloo, Ontario, Canada
Genome Biol 10:214. 2009..b>Orc1-5 appear to be present in all eukaryotes, and include both AAA+ and winged-helix motifs...
- Redundant and differential regulation of multiple licensing factors ensures prevention of re-replication in normal human cellsNozomi Sugimoto
Virology Division, National Cancer Center Research Institute, Chuohku, Tokyo 104 0045, Japan
J Cell Sci 122:1184-91. 2009..cells enter S-phase, overlapping differential inhibitory mechanisms downregulate the replication licensing factors ORC1, CDC6 and Cdt1...
- An episomal mammalian replicon: sequence-independent binding of the origin recognition complexDaniel Schaarschmidt
Department of Biology, Universitat Konstanz, Konstanz, Germany
EMBO J 23:191-201. 2004..immunoprecipitations and quantitative polymerase chain reaction analysis revealed that, in G1-phase cells, Orc1 and Orc2, as well as Mcm3, another component of the prereplication complex, are bound to multiple sites on the ..
- Novel DNA binding motifs in the DNA repair enzyme endonuclease III crystal structureM M Thayer
Scripps Research Institute, Department of Molecular Biology MB4, La Jolla, CA 92037, USA
EMBO J 14:4108-20. 1995..Helix-hairpin-helix (HhH) and [4Fe-4S] cluster loop (FCL) motifs, which we have named for their secondary structure, bracket the cleft ..
- The conserved bromo-adjacent homology domain of yeast Orc1 functions in the selection of DNA replication origins within chromatinPhilipp Müller
Department of Biomolecular Chemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Genes Dev 24:1418-33. 2010..This study focused on the role of the conserved N-terminal bromo-adjacent homology domain of yeast Orc1 (Orc1BAH). Recent studies indicate that BAH domains are chromatin-binding modules...
- A novel mutation, P126R, in a Japanese patient with HHH syndromeTakeshi Miyamoto
Department of Inherited Metabolic Disease, National Institute of Neuroscience, NCNP, 4 1 1 Ogawahigashi, Kodaira, Tokyo 187 8502, Japan
Pediatr Neurol 26:65-7. 2002..Several mutations have been reported in the ORNT1 gene encoding mitochondrial ornithine transporter of patients with this disorder...
- Function of the origin recognition complex 1 (ORC1) outside DNA replication in DrosophilaSo Young Park
The Ohio State Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA
Cell Cycle 10:3957-63. 2011..Levels of the largest ORC subunit, ORC1, oscillate during the mitotic cell cycle and regulate origin usage...
- Protection of repetitive DNA borders from self-induced meiotic instabilityGerben Vader
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
Nature 477:115-9. 2011..border-specific protection system consisting of the meiotic ATPase Pch2 and the origin recognition complex subunit Orc1. Upon disruption of these factors, DSB formation and recombination increased specifically in the outermost rDNA ..
- Single Qdot-labeled glycosylase molecules use a wedge amino acid to probe for lesions while scanning along DNAAndrew R Dunn
The Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA
Nucleic Acids Res 39:7487-98. 2011..DNA glycosylase (Fpg) and endonuclease VIII (Nei) are members of two glycosylase families: the helix-hairpin-helix (HhH) superfamily and the Fpg/Nei family...
- Different roles of the human Orc6 protein in the replication initiation processAndreas W Thomae
Department of Gene Vectors, Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Munich, Germany
Cell Mol Life Sci 68:3741-56. 2011..We show that Orc6 not only interacts with Orc1-Orc5 but also with the initiation factor Cdc6...
- Advance directives in home health and hospice agencies: United States, 2007Helaine E Resnick
American Association of Homes and Services for the Aging, Washington, DC, USA
Am J Hosp Palliat Care 28:467-74. 2011..data on policies, storage, and implementation of advance directives (ADs) in home health and hospice (HHH) agencies in the United States using the National Home and Hospice Care Survey...
- Can hydridic-to-protonic hydrogen bonds catalyze hydride transfers in biological systems?Simona Marincean
Natural Sciences Department, University of Michigan Dearborn, Dearborn, Michigan 48128 1491, United States
J Phys Chem A 114:13376-80. 2010Catalysis of hydride transfer by hydridic-to-protonic hydrogen (HHH) bonding in α-hydroxy carbonyl isomerization reactions was examined computationally in the lithium salts of 7-substituted endo-3-hydroxybicyclo[2.2.1]hept-5-en-2-ones...
- RBX1 (RING box protein 1) E3 ubiquitin ligase is required for genomic integrity by modulating DNA replication licensing proteinsLijun Jia
Division of Radiation and Cancer Biology, Department of Radiation Oncology, University of Michigan, Ann Arbor, Michigan 48109, USA
J Biol Chem 286:3379-86. 2011..that, in human cancer cells, RBX1 silencing causes the accumulation of DNA replication licensing proteins CDT1 and ORC1, leading to DNA double-strand breaks, DDR, G(2) arrest, and, eventually, aneuploidy...
- Hyperornithinemia, hyperammonemia, and homocitrullinuria syndrome with evidence of mitochondrial dysfunction due to a novel SLC25A15 (ORNT1) gene mutation in a Palestinian familyStanley H Korman
Department of Clinical Biochemistry, Hadassah Hebrew University Hospital Medical Center, PO Box 12000, Jerusalem 91120, Israel
J Neurol Sci 218:53-8. 2004Hyperornithinemia, hyperammonemia, and homocitrullinuria (HHH) syndrome is caused by mutations in the SLC25A15 (ORNT1) gene encoding the mitochondrial ornithine transporter, but the mechanism of pathogenesis of the encephalopathy, spastic ..
- Estrogen receptor α (ERα) mediates 17β-estradiol (E2)-activated expression of HBO1Wen zhong Wang
Department of Biochemistry and Molecular Biology, Shanghai Medical College of Fudan University, Shanghai, 200032, China
J Exp Clin Cancer Res 29:140. 2010HBO1 (histone acetyltransferase binding to ORC1) is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer...
- Involvement of RAD9-dependent damage checkpoint control in arrest of cell cycle, induction of cell death, and chromosome instability caused by defects in origin recognition complex in Saccharomyces cerevisiaeKeiichi Watanabe
Department of Molecular Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, Ikoma, Nara 630 0101, Japan
Eukaryot Cell 1:200-12. 2002..The cell cycle of orc1-4/orc1-4 temperature-sensitive mutant was arrested at the G2/M boundary, after several rounds of cell division at ..
- Three novel mutations (G27E, insAAC, R179X) in the ORNT1 gene of Japanese patients with hyperornithinemia, hyperammonemia, and homocitrullinuria syndromeS Tsujino
Department of Inherited Metabolic Disease, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan
Ann Neurol 47:625-31. 2000..We report three novel mutations in the mitochondrial ornithine transporter gene (ORNT1) of Japanese patients with HHH syndrome: a nonsense mutation (R179X) associated with exon skipping and a ..
- Human origin recognition complex is essential for HP1 binding to chromatin and heterochromatin organizationSupriya G Prasanth
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
Proc Natl Acad Sci U S A 107:15093-8. 2010..Fluorescent bleaching studies indicate that multiple subcomplexes of ORC exist at heterochromatin, with Orc1 stably associating with heterochromatin in G1 phase, whereas other ORC subunits have transient interactions ..
- The multidomain structure of Orc1p reveals similarity to regulators of DNA replication and transcriptional silencingS P Bell
Cold Spring Harbor Laboratory, New York 11724, USA
Cell 83:563-8. 1995..Here we report the cloning of the genes encoding the 120 kDa (ORC1), 62 kDa (ORC3), and 56 kDa (ORC4) subunits of ORC and the reconstitution of the complete complex after expression ..
- Regulation of cell growth-dependent expression of mammalian CDC6 gene by the cell cycle transcription factor E2FK Ohtani
Human Gene Sciences Center, Graduate School of Dentistry, Tokyo Medical and Dental University, Japan
Oncogene 17:1777-85. 1998..or HsOrc1 failed to induce DNA synthesis unlike overexpression of E2F1, even though E2F1 induced both Cdc6 and Orc1, suggesting that E2F may regulate the expression of another gene(s), besides Cdc6 and Orc1, required for induction ..
- The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replicationMasashi Uchiyama
Department of Pathology, Stanford University School of Medicine, MED CTR R 272, Stanford, CA 94305 5324, USA
Mol Cell Biol 24:7419-34. 2004..that the fission yeast Spb70 genetically interacts and coprecipitates with origin recognition complex proteins Orp1/Orc1 and Orp2/Orc2 and primase coupling subunit Spp2/p58...
- Complete genome sequence of the anaerobic, protein-degrading hyperthermophilic crenarchaeon Desulfurococcus kamchatkensisNikolai V Ravin
Centre Bioengineering, Russian Academy of Sciences, Moscow, Russia
J Bacteriol 191:2371-9. 2009..Unlike in most archaea, two genes encoding homologs of the eukaryotic initiator proteins Orc1 and Cdc6 are located distantly from this site...
- A novel R275X mutation of the SLC25A15 gene in a Japanese patient with the HHH syndromeHiroyuki Torisu
Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
Brain Dev 28:332-5. 2006..Mutations in the SLC25A15 gene have been coupled to the HHH syndrome...
- ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNAChristian Speck
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nat Struct Mol Biol 12:965-71. 2005..to ORC on DNA in an ATP-dependent manner, which induces a change in the pattern of origin binding that requires the Orc1 ATPase...
- Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteinsK C Thome
Division of Molecular Oncology, Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 275:35233-41. 2000..Finally, all ORC subunits remain consistently nuclear, and Orc2 is consistently phosphorylated through all stages of the cell cycle, whereas Orc1 is selectively phosphorylated in mitosis.
- Replication factors MCM2 and ORC1 interact with the histone acetyltransferase HBO1T W Burke
Department of Genetics, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 276:15397-408. 2001..One of the proteins we identified is identical to the ORC1-interacting protein termed HBO1...
- Arginine vasopressin as a supplementary vasopressor in refractory hypertensive, hypervolemic, hemodilutional therapy in subarachnoid hemorrhageSusanne Muehlschlegel
Division of Vascular Neurology and Neurocritical Care, Department of Neurology, Massachusetts General Hospital Brigham Women s Hospital, Harvard Medical School, 55 Fruit Street, Boston, MA 02114, USA
Neurocrit Care 6:3-10. 2007Hypertensive, hypervolemic, and hemodilutional (HHH) therapy for vasospasm in subarachnoid hemorrhage (SAH) refractory to phenylephrine requires high doses of catecholamines, leading to adverse adrenergic effects...
- Genome-wide localization of pre-RC sites and identification of replication origins in fission yeastMakoto Hayashi
Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan
EMBO J 26:1327-39. 2007..Here, we determined locations of Orc1 and Mcm6, components of pre-replicative complex (pre-RC), on the whole genome of Schizosaccharomyces pombe using a ..
- An essential role for Orc6 in DNA replication through maintenance of pre-replicative complexesJeffrey W Semple
Department of Biology, University of Waterloo, Waterloo, Ontario, Canada
EMBO J 25:5150-8. 2006..Only the Orc1-5 subunits appear to be required for origin binding in budding yeast, yet Orc6 is an essential protein for cell ..
- Clinical trials update from the joint European Society and World Congress of Cardiology meeting: PEP-CHF, ACCLAIM and the HHH studyJohn G F Cleland
Department of Cardiology, University of Hull, Castle Hill Hospital, Cottingham, Kingston upon Hull, HU15 5JQ, UK
Eur J Heart Fail 8:658-61. 2006..The HHH study failed to show a beneficial effect of telemonitoring over usual care in patients with heart failure but ..
- The structure of the human ERCC1/XPF interaction domains reveals a complementary role for the two proteins in nucleotide excision repairKonstantinos Tripsianes
Department of NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
Structure 13:1849-58. 2005..Both domains exhibit the double helix-hairpin-helix motif (HhH)2, and they are related by a pseudo-2-fold symmetry axis...
- Biochemical analysis of a DNA replication origin in the archaeon Aeropyrum pernixIan Grainge
Cancer Research UK, Clare Hall Laboratories, The London Research Institute, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3LD, UK
J Mol Biol 363:355-69. 2006We have characterised the interaction of the Aeropyrum pernix origin recognition complex proteins (ORC1 and ORC2) with DNA using DNase I footprinting. Each protein binds upstream of its respective gene...
- Differential binding of replication proteins across the human c-myc replicatorMaloy Ghosh
Department of Biochemistry and Molecular Biology, Wright State University, 3640 Colonel Glenn Highway, Dayton, Ohio 45435, USA
Mol Cell Biol 26:5270-83. 2006The binding of the prereplication complex proteins Orc1, Orc2, Mcm3, Mcm7, and Cdc6 and the novel DNA unwinding element (DUE) binding protein DUE-B to the endogenous human c-myc replicator was studied by chromatin immunoprecipitation...
- Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complexIlian Radichev
NICHD, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
J Biol Chem 281:23264-73. 2006..Moreover, only ORC-(1-5) was bound to the chromatin fraction, suggesting that Orc1 is required in vivo to load ORC-(2-5) onto chromatin...
- Molecular Basis of OTC Deficiency and Related DisordersMendel Tuchman; Fiscal Year: 2005..properties of human native and recombinant wild type mitochondrial ornithine carrier (ORNT) and mutants causing HHH syndrome (a) Study the oligomeric structure of ORNT by using hydrodynamic analysis, gel filtration, charge shift ..
- Structure/Function Studies of DNA Replication InitiationJames Berger; Fiscal Year: 2007..Specifically, we aim to: 1) biochemically and structurally determine how the archaeal Cdc6/Orc1 protein interacts with specific replication origin sites, 2) reconstitute and biophysically characterize dimeric ..
- New Perspectives in Transporter Biology (FASEB Conference)Michael Robinson; Fiscal Year: 2005..attention deficit hyperactivity disorder (ADHD), diabetes, orthostatic hypotension, cystinuria, cystic fibrosis, HHH syndrome, etc; there is a growing list of 'transporteropathies'...
- Optimization of hybrid enzymes for DNA technologiesAlexei Slesarev; Fiscal Year: 2006..Fidelity Systems has successfully pursued this strategy by recognizing the role of helixhairpin- helix (HhH) protein domains in sequence non-specific interactions with DNA...
- CELL CYCLE OF PLASMODIUM FALCIPARUMDebopam Chakrabarti; Fiscal Year: 2003..falciparum homologues of MCM4, ORC1 and ORC5. (b) Identify proteins interacting with P...
- DNA POLYMERASE BETA AND MUTAGENESISJoann B Sweasy; Fiscal Year: 2010..synthesis are located within regions of Pol ss other than the hydrophobic hinge, loop II, and the region between HhH motifs and to study mechanisms used by specific regions of Pol ss that are critical for accurate DNA synthesis...
- SLEEP HEART HEALTH STUDYHelaine Resnick; Fiscal Year: 2007..Accomplishing these goals will provide important information concerning the public health impact of SDB. ..
- INTERNATIONAL COREIgor Grant; Fiscal Year: 2007..abstract_text> ..
- Role of Arginase in Inflammation and TraumaSIDNEY MACHEN MORRIS; Fiscal Year: 2010..Results of these studies will provide a basis for rational development of new and more effective therapeutic strategies and for monitoring efficacy of treatment in inflammatory diseases. ..
- Alzheimer Caregiver Coping: Mental and Physical HealthIgor Grant; Fiscal Year: 2010..This study will provide a basis for a mechanistic understanding of the path from CG stress to CV morbidity in elderly CG, and opens the possibility of targeted interventions to reduce this risk. ..
- Alzheimer Caregiver Coping: Mental and Physical HealthIgor Grant; Fiscal Year: 2007..This study will provide a basis for a mechanistic understanding of the path from CG stress to CV morbidity in elderly CG, and opens the possibility of targeted interventions to reduce this risk. ..
- NEW INHIBITORS FOR THE PENICILLIN BINDING PROTEINSWILLIAM GUTHEIL; Fiscal Year: 2002..These studies will established improved methods for the study of the PBPs, determine essential features of PBP substrate specificity, and use this information to design new PBP inhibitors. ..
- ARGINASE AND INFLAMMATION AND TRAUMASidney Morris; Fiscal Year: 2001..The third aim is to elucidate the physiologic roles of Arg II in vivo. The investigator will apply tools of modern molecular biology and genetics to address these questions. ..
- Cell cycle control by ORC1 and other E2F targetsMaki Asano; Fiscal Year: 2005..Three specific aims will be pursued. (1) Identification of the mechanisms underlying ORC1 degradation...
- California NeuroAIDS Tissue NetworkIgor Grant; Fiscal Year: 2007..The CNTN will coordinate its work closely with that of the other NNTC sites to assure that the entire NNTC develops as a national and international resource for research on neuroAIDS. ..
- DNA replication checkpoints in fission yeastGENNARO D URSO; Fiscal Year: 2007..Moreover, our results will likely identify new drug targets for cancer therapy, and reveal potentially less mutagenic strategies to block cell cycle progression in cancer cells. ..
- Role of Arginase in Inflammation and TraumaSIDNEY MACHEN MORRIS; Fiscal Year: 2011..Results of these studies will provide a basis for rational development of new and more effective therapeutic strategies and for monitoring efficacy of treatment in inflammatory diseases. ..