SHMT2

Summary

Gene Symbol: SHMT2
Description: serine hydroxymethyltransferase 2
Alias: GLYA, HEL-S-51e, SHMT, serine hydroxymethyltransferase, mitochondrial, GLY A+, epididymis secretory sperm binding protein Li 51e, glycine auxotroph A, human complement for hamster, glycine hydroxymethyltransferase, serine aldolase, serine hydroxymethylase, serine hydroxymethyltransferase 2 (mitochondrial), serine methylase, threonine aldolase
Species: human
Products:     SHMT2

Top Publications

  1. Garrow T, Brenner A, Whitehead V, Chen X, Duncan R, Korenberg J, et al. Cloning of human cDNAs encoding mitochondrial and cytosolic serine hydroxymethyltransferases and chromosomal localization. J Biol Chem. 1993;268:11910-6 pubmed
    Human cDNAs for cytosolic and mitochondrial serine hydroxymethyltransferase (SHMT) were cloned by functional complementation of an Escherichia coli glyA mutant with a human cDNA library...
  2. Stover P, Chen L, Suh J, Stover D, Keyomarsi K, Shane B. Molecular cloning, characterization, and regulation of the human mitochondrial serine hydroxymethyltransferase gene. J Biol Chem. 1997;272:1842-8 pubmed
    ..Mitochondrial SHMT mRNA levels in MCF-7 cells did not vary during the cell cycle and were not affected by the absence of glycine, ..
  3. Girgis S, Nasrallah I, Suh J, Oppenheim E, Zanetti K, Mastri M, et al. Molecular cloning, characterization and alternative splicing of the human cytoplasmic serine hydroxymethyltransferase gene. Gene. 1998;210:315-24 pubmed
    ..reading frame is interrupted by 10 introns, two of which are positionally conserved within the human mitochondrial SHMT gene. The gene is expressed with 330 nucleotides of 5' untranslated message within three exons...
  4. Perry C, Yu S, Chen J, Matharu K, Stover P. Effect of vitamin B6 availability on serine hydroxymethyltransferase in MCF-7 cells. Arch Biochem Biophys. 2007;462:21-7 pubmed
    ..serine through the activity of the pyridoxal-phosphate (PLP)-dependent isozymes of serine hydroxymethyltransferase (SHMT)...
  5. Anderson D, Stover P. SHMT1 and SHMT2 are functionally redundant in nuclear de novo thymidylate biosynthesis. PLoS ONE. 2009;4:e5839 pubmed publisher
    ..This residual activity was due to the presence of a cytoplasmic/nuclear isozyme of SHMT encoded by Shmt2. Shmt2 is shown to encode two transcripts, one which encodes a protein that localizes exclusively to the ..
  6. Galland C, Dupuy C, Loizeau V, Danion M, Auffret M, Quiniou L, et al. Proteomic analysis of the European flounder Platichthys flesus response to experimental PAH-PCB contamination. Mar Pollut Bull. 2015;95:646-57 pubmed publisher
    ..GPx) proteins as well as of several proteins belonging to the betaine demethylation pathway and the methionine cycle (BHMT, SHMT, SAHH), suggesting a role for these different pathways in the P. flesus response to chemical contamination.
  7. Milano T, di Salvo M, Angelaccio S, Pascarella S. Conserved water molecules in bacterial serine hydroxymethyltransferases. Protein Eng Des Sel. 2015;28:415-26 pubmed publisher
    ..This work provides a map of the conserved water molecules helpful for deciphering SHMT mechanism and for rational design of molecular engineering experiments.
  8. Misselbeck K, Marchetti L, Priami C, Stover P, Field M. The 5-formyltetrahydrofolate futile cycle reduces pathway stochasticity in an extended hybrid-stochastic model of folate-mediated one-carbon metabolism. Sci Rep. 2019;9:4322 pubmed publisher
    ..enzymes phosphoribosylaminoimidazolecarboxamide formyltransferase (AICARFT) and serine hydroxymethyltransferase (SHMT)...
  9. de Boer P, Rahaoui H, Leer R, Montijn R, van der Vossen J. Real-time PCR detection of Campylobacter spp.: A comparison to classic culturing and enrichment. Food Microbiol. 2015;51:96-100 pubmed publisher
    ..PCR detection methods: one targeting the Campylobacter jejuni hipO gene, one targeting the Campylobacter coli glyA gene, and one generically targeting Campylobacter spp. 16S rDNA sequence...

More Information

Publications119 found, 100 shown here

  1. Cao J, Sun L, Aramsangtienchai P, Spiegelman N, Zhang X, Huang W, et al. HDAC11 regulates type I interferon signaling through defatty-acylation of SHMT2. Proc Natl Acad Sci U S A. 2019;116:5487-5492 pubmed publisher
    ..Through proteomics studies, we hypothesized and later biochemically validated SHMT2 as a defatty-acylation substrate of HDAC11...
  2. Tokes Fuzesi M, Ruzsics I, Rideg O, Kustán P, Kovacs G, Molnar T. Role of microparticles derived from monocytes, endothelial cells and platelets in the exacerbation of COPD. Int J Chron Obstruct Pulmon Dis. 2018;13:3749-3757 pubmed publisher
    ..EMPs (CD31+, CD62E+) and platelet-derived (CD61+, CD41+, CD42a+, PAC1+), red blood cell-derived (GlyA+) and leukocyte-derived (CD45+, CD13+, CD14+, CD56+) MPs were measured...
  3. Nonaka H, Nakanishi Y, Kuno S, Ota T, Mochidome K, Saito Y, et al. Design strategy for serine hydroxymethyltransferase probes based on retro-aldol-type reaction. Nat Commun. 2019;10:876 pubmed publisher
    Serine hydroxymethyltransferase (SHMT) is an enzyme that catalyzes the reaction that converts serine to glycine. It plays an important role in one-carbon metabolism. Recently, SHMT has been shown to be associated with various diseases...
  4. Lin C, Huang M, Lin C, Huang C, Lane H. Altered mRNA expressions for N-methyl-D-aspartate receptor-related genes in WBC of patients with major depressive disorder. J Affect Disord. 2019;245:1119-1125 pubmed publisher
    ..Expressions of NMDAR-related genes including SRR, SHMT2, PSAT1, GCAT, GAD1, SLC1A4, NRG1 and COMT in peripheral WBCs of 110 patients with MDD (25 drug-naïve, 21 drug-..
  5. Lakhssassi N, Patil G, Piya S, Zhou Z, Baharlouei A, Kassem M, et al. Genome reorganization of the GmSHMT gene family in soybean showed a lack of functional redundancy in resistance to soybean cyst nematode. Sci Rep. 2019;9:1506 pubmed publisher
    ..This work presents a comprehensive study of the SHMT gene family members, including synteny, phylogeny, subcellular localizations, haplotypes, protein homology modeling,..
  6. Lin C, Chen Y, Bai Y, Zhang Y. Long noncoding RNA LINC01234 promotes serine hydroxymethyltransferase 2 expression and proliferation by competitively binding miR-642a-5p in colon cancer. Cell Death Dis. 2019;10:137 pubmed publisher
    ..of LINC01234 on colon cancer cell proliferation and its regulatory mechanism of serine hydroxymethyltransferase 2 (SHMT2) by acting as a competing endogenous RNA (ceRNA)...
  7. Kim H, Jung W, Koo J. Site-specific metabolic phenotypes in metastatic breast cancer. J Transl Med. 2014;12:354 pubmed publisher
    ..PSAT), phosphoserine phosphatase (PSPH), glycine decarboxylase (GLDC), and serine hydroxymethyltransferase (SHMT)...
  8. Tabib A, Hindi I, Karbian N, Zelig O, Falach B, Mevorach D. Prothrombotic mechanisms in patients with congenital p.Cys89Tyr mutation in CD59. Thromb Res. 2018;168:67-77 pubmed publisher
    ..5-0.9 μm, were characterized (Annexin V, anti-human GlyA, anti-CD15, anti-CD14, anti-CD61). Platelet-monocyte aggregation was assessed with FlowSight...
  9. Tramonti A, Paiardini A, Paone A, Bouzidi A, Giardina G, Guiducci G, et al. Differential inhibitory effect of a pyrazolopyran compound on human serine hydroxymethyltransferase-amino acid complexes. Arch Biochem Biophys. 2018;653:71-79 pubmed publisher
    ..It exists in cytosolic (SHMT1) and mitochondrial (SHMT2) isoforms...
  10. Wei Z, Song J, Wang G, Cui X, Zheng J, Tang Y, et al. Deacetylation of serine hydroxymethyl-transferase 2 by SIRT3 promotes colorectal carcinogenesis. Nat Commun. 2018;9:4468 pubmed publisher
    The conversion of serine and glycine that is accomplished by serine hydroxymethyltransferase 2 (SHMT2) in mitochondria is significantly upregulated in various cancers to support cancer cell proliferation...
  11. He Y, Wang L, Ma W, Lu X, Li Y, Liu J. Secretory expression, immunoaffinity purification and metal-binding ability of recombinant metallothionein (ShMT) from freshwater crab Sinopotamon henanense. Ecotoxicol Environ Saf. 2019;169:457-463 pubmed publisher
    ..Sinopotamon henanense is a decapod crustacean widely distributed in northern China, in which only one MT isoform (ShMT) has been reported so far...
  12. Zhang H, Liu C, Han Y, Ma Z, Zhang H, Ma Y, et al. Genetic variations in the one-carbon metabolism pathway genes and susceptibility to hepatocellular carcinoma risk: a case-control study. Tumour Biol. 2015;36:997-1002 pubmed publisher
    ..Then eight SNPs from seven OCM genes (MTHFR, MTR, MTRR, FTHFD, GART, SHMT, and CBS) were evaluated...
  13. Dong Y, Huo X, Sun R, Liu Z, Huang M, Yang S. lncRNA Gm15290 promotes cell proliferation and invasion in lung cancer through directly interacting with and suppressing the tumor suppressor miR-615-5p. Biosci Rep. 2018;38: pubmed publisher
    ..and increased the protein levels of miR-615-5p target genes, including IGF2, AKT2, and SHMT2 Moreover, miR-615-5p mimic could antagonize the promoting effect of Gm15290 on cell proliferation and ..
  14. Liu Y, Sowell S, Luo Y, Chaubey A, Cameron R, Kim H, et al. Autism and Intellectual Disability-Associated KIRREL3 Interacts with Neuronal Proteins MAP1B and MYO16 with Potential Roles in Neurodevelopment. PLoS ONE. 2015;10:e0123106 pubmed publisher
    ..expressed proteins MAP1B and MYO16 and its intracellular domain can potentially interact with ATP1B1, UFC1, and SHMT2. The interactions were confirmed by co-immunoprecipitation and colocalization analyses of proteins expressed in ..
  15. Angelucci F, Morea V, Angelaccio S, Saccoccia F, Contestabile R, Ilari A. The crystal structure of archaeal serine hydroxymethyltransferase reveals idiosyncratic features likely required to withstand high temperatures. Proteins. 2014;82:3437-49 pubmed publisher
    ..We determined the crystal structure of free (apo) and pyridoxal-5'-phosphate-bound (holo) SHMT from Methanocaldococcus jannaschii, the first from a hyperthermophile, from the archaea domain of life and that ..
  16. Gandhi S, Gaur N, Krishna S, Siddiqi M, Saxena J. Arg-265: a critical residue of L.donovani cytosolic SHMT in maintaining the binding of THF and catalysis. Exp Parasitol. 2015;149:16-23 pubmed publisher
    ..These results suggested that Arg265 residue is required for the binding of Tetrahydrofolate and may be the base that abstracts α-proton from glycine, leading to formation of quinonoid intermediate in cytosolic SHMT of L. donovani.
  17. Alonso R, Girbau C, Martinez Malaxetxebarria I, Fernández Astorga A. Multilocus sequence typing reveals genetic diversity of foodborne Arcobacter butzleri isolates in the North of Spain. Int J Food Microbiol. 2014;191:125-8 pubmed publisher
    ..A total of 157 alleles were identified across all seven loci, ranging from 16 alleles at glnA to 31 at glyA. MLST differentiated the isolates into 34 sequence types (STs), with the majority of isolates containing a unique ..
  18. Machover D, Goldschmidt E, Mollicone R, Haghighi Rad F, Desterke C, Gaston Mathe Y, et al. Enhancement of 5-Fluorouracil Cytotoxicity by Pyridoxal 5'-Phosphate and Folinic Acid in Tandem. J Pharmacol Exp Ther. 2018;366:238-243 pubmed publisher
    ..The hypothesis relates to the low affinity for cofactor of the PLP-dependent serine hydroxymethyl transferase (SHMT), the enzyme that catalyzes formation of CH2-H4PteGlu by transfer of the C? of serine ..
  19. Nambiar D, Berhane T, Shew R, Schwarz B, Duff M, Howell E. In Vivo Titration of Folate Pathway Enzymes. Appl Environ Microbiol. 2018;84: pubmed publisher
    ..reductase clone rescuing a ΔmetF strain, and for a serine hydroxymethyltransferase clone rescuing a ΔglyA strain. Additionally, an R67 dihydrofolate reductase clone allowed E...
  20. Waditee Sirisattha R, Kageyama H, Tanaka Y, Fukaya M, Takabe T. Overexpression of halophilic serine hydroxymethyltransferase in fresh water cyanobacterium Synechococcus elongatus PCC7942 results in increased enzyme activities of serine biosynthetic pathways and enhanced salinity tolerance. Arch Microbiol. 2017;199:29-35 pubmed publisher
    Serine hydroxymethyltransferase (SHMT) catalyzes the conversion of serine to glycine and provides activated one-carbon units required for synthesis of nucleic acids, proteins and numerous biological compounds...
  21. Xu M, Moresco J, Chang M, Mukim A, Smith D, Diedrich J, et al. SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy. PLoS Pathog. 2018;14:e1007071 pubmed publisher
    ..of whole-cell extracts from 2D10 Jurkat T cells revealed that JIB-04 targets Serine Hydroxymethyltransferase 2 (SHMT2), a regulator of glycine biosynthesis and an adaptor for the BRCC36 K63Ub-specific deubiquitinase in the BRISC ..
  22. Núñez Díaz J, Fumanal M, do Vale A, Fernández Díaz C, Moriñigo M, Balebona M. Transcription of IVIAT and Virulence Genes in Photobacterium damselae Subsp. piscicida Infecting Solea senegalensis. Microorganisms. 2018;6: pubmed publisher
    ..HutB and HutD), oxidative stress defence (AhpC and Sod), adhesion (PDP_0080), toxins (AIP56) and metabolism (Impdh, Shmt and AlaRS) were detected in Phdp infecting S. senegalensis head kidney or liver...
  23. Kumar A, Wu G, Liu Z. Synthesis and characterization of cross linked enzyme aggregates of serine hydroxyl methyltransferase from Idiomerina leihiensis. Int J Biol Macromol. 2018;117:683-690 pubmed publisher
    A thermo-stable purified serine hydroxymethyltransferase (SHMT; 418 AA) was used for the carrier free immobilization using pectin as a coach molecule and formaldehyde as a cross-linker...
  24. Chitnumsub P, Jaruwat A, Riangrungroj P, Ittarat W, Noytanom K, Oonanant W, et al. Structures of Plasmodium vivax serine hydroxymethyltransferase: implications for ligand-binding specificity and functional control. Acta Crystallogr D Biol Crystallogr. 2014;70:3177-86 pubmed publisher
    ..Serine hydroxymethyltransferase (SHMT) from Plasmodium spp...
  25. Winkler F, Kriebel M, Clever M, Gröning S, Großhans J. Essential Function of the Serine Hydroxymethyl Transferase (SHMT) Gene During Rapid Syncytial Cell Cycles in Drosophila. G3 (Bethesda). 2017;7:2305-2314 pubmed publisher
    ..The serine hydroxymethyl transferase (SHMT) catalyzing the conversion of serine and glycine and vice versa feeds into tetrahydrofolate (THF)-mediated C1 ..
  26. Paone A, Marani M, Fiascarelli A, Rinaldo S, Giardina G, Contestabile R, et al. SHMT1 knockdown induces apoptosis in lung cancer cells by causing uracil misincorporation. Cell Death Dis. 2014;5:e1525 pubmed publisher
    ..While the mitochondrial isoform of SHMT (SHMT2) has recently been identified as an important player in the control of cell proliferation in several cancer types ..
  27. Maenpuen S, Amornwatcharapong W, Krasatong P, Sucharitakul J, Palfey B, Yuthavong Y, et al. Kinetic mechanism and the rate-limiting step of Plasmodium vivax serine hydroxymethyltransferase. J Biol Chem. 2015;290:8656-65 pubmed publisher
    Serine hydroxymethyltransferase (SHMT) is a pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes a hydroxymethyl group transfer from L-serine to tetrahydrofolate (H4folate) to yield glycine and 5,10-methylenetetrahydrofolate (CH2-..
  28. Banting G, Braithwaite S, Scott C, Kim J, Jeon B, Ashbolt N, et al. Evaluation of Various Campylobacter-Specific Quantitative PCR (qPCR) Assays for Detection and Enumeration of Campylobacteraceae in Irrigation Water and Wastewater via a Miniaturized Most-Probable-Number-qPCR Assay. Appl Environ Microbiol. 2016;82:4743-4756 pubmed publisher
    ..5% by the Van Dyke 16S and Jensen glyA qPCR assays, and 75% by the Linton 16S endpoint PCR when cultured at 37°C...
  29. Ramírez Escudero M, Del Pozo M, Marin Navarro J, Gonzalez B, Golyshin P, Polaina J, et al. Structural and Functional Characterization of a Ruminal ?-Glycosidase Defines a Novel Subfamily of Glycoside Hydrolase Family 3 with Permuted Domain Topology. J Biol Chem. 2016;291:24200-24214 pubmed
    ..Herein, we describe a GH3 enzyme (GlyA1) from resident microbial communities in strained ruminal fluid...
  30. Ye J, Fan J, Venneti S, Wan Y, Pawel B, Zhang J, et al. Serine catabolism regulates mitochondrial redox control during hypoxia. Cancer Discov. 2014;4:1406-17 pubmed publisher
    ..In this study, we demonstrate that the serine catabolic enzyme, mitochondrial serine hydroxymethyltransferase (SHMT2), is induced when MYC-transformed cells are subjected to hypoxia...
  31. Zeqiraj E, Tian L, Piggott C, Pillon M, Duffy N, Ceccarelli D, et al. Higher-Order Assembly of BRCC36-KIAA0157 Is Required for DUB Activity and Biological Function. Mol Cell. 2015;59:970-83 pubmed publisher
    ..into a dimer of heterodimers (super dimers) was required for DUB activity and interaction with targeting proteins SHMT2 and RAP80...
  32. Pasini M, Fernández Castané A, Jaramillo A, de Mas C, Caminal G, Ferrer P. Using promoter libraries to reduce metabolic burden due to plasmid-encoded proteins in recombinant Escherichia coli. N Biotechnol. 2016;33:78-90 pubmed publisher
    ..coli expression with an antibiotic-free plasmid maintenance mechanism based on a glycine auxotrophic marker (glyA)...
  33. Wu X, Zhou G, Chen Y, Wu P, Liu L, Ma F, et al. Soybean Cyst Nematode Resistance Emerged via Artificial Selection of Duplicated Serine Hydroxymethyltransferase Genes. Front Plant Sci. 2016;7:998 pubmed publisher
    ..which controls resistance to soybean cyst nematodes (SCN), encodes the enzyme serine hydroxylmethyltransferase (SHMT)...
  34. Kumar A, Wu G, Wu Z, Kumar N, Liu Z. Improved catalytic properties of a serine hydroxymethyl transferase from Idiomarina loihiensis by site directed mutagenesis. Int J Biol Macromol. 2018;117:1216-1223 pubmed publisher
    A novel glyA gene was screened from a marine bacterium, Idiomarina loihiensis encoding a thermo-stable serine hydroxymethyl transferase (SHMT; 418 AA; 45.4 kDa)...
  35. Komatsu H, Furuya Y, Sawada K, Asada T. Involvement of the strychnine-sensitive glycine receptor in the anxiolytic effects of GlyT1 inhibitors on maternal separation-induced ultrasonic vocalization in rat pups. Eur J Pharmacol. 2015;746:252-7 pubmed publisher
    ..There are two types of glycine receptor: the strychnine-sensitive glycine receptor (GlyA) and the strychnine-insensitive glycine receptor (GlyB); however, which receptor is the main contributor to the ..
  36. Wan Y. New Mechanism of Bone Cancer Pain: Tumor Tissue-Derived Endogenous Formaldehyde Induced Bone Cancer Pain via TRPV1 Activation. Adv Exp Med Biol. 2016;904:41-58 pubmed publisher
    ..cancer cells produced formaldehyde through demethylation process by serine hydroxymethyltransferase (SHMT1 and SHMT2) and lysine-specific histone demethylase 1 (LSD1)...
  37. Mokkala K, Pellonperä O, Röytiö H, Pussinen P, Rönnemaa T, Laitinen K. Increased intestinal permeability, measured by serum zonulin, is associated with metabolic risk markers in overweight pregnant women. Metabolism. 2017;69:43-50 pubmed publisher
    ..for metabolic endotoxemia (LPS), inflammation (high-sensitive C-reactive protein and glycoprotein acetylation GlyA), glucose metabolism (fasting glucose and insulin), and lipid metabolism were measured...
  38. Jiang W, Chen L, Hu N, Yuan S, Li B, Liu Z. A novel serine hydroxymethyltransferase from Arthrobacter nicotianae: characterization and improving catalytic efficiency by rational design. BMC Biotechnol. 2014;14:93 pubmed publisher
    ..Here, a novel SHMT gene, glyA, was obtained through degenerate oligonucleotide-primed PCR and encoded a novel SHMT with 54...
  39. Liu S, Lucas K, Roy S, Ha J, Raikhel A. Mosquito-specific microRNA-1174 targets serine hydroxymethyltransferase to control key functions in the gut. Proc Natl Acad Sci U S A. 2014;111:14460-5 pubmed publisher
    ..We identified serine hydroxymethyltransferase (SHMT) is a direct miR-1174 target...
  40. Naushad S, Vijayalakshmi S, Rupasree Y, Kumudini N, Sowganthika S, Naidu J, et al. Multifactor dimensionality reduction analysis to elucidate the cross-talk between one-carbon and xenobiotic metabolic pathways in multi-disease models. Mol Biol Rep. 2015;42:1211-24 pubmed publisher
    ..Gene-gene interactions within one-carbon metabolic pathway were observed in CAD (GCPII 1561 C>T, SHMT 1420 C>T and MTHFR 677 C>T) and PD (cSHMT 1420 C>T, MTRR 66 A>G and RFC1 80 G>A)...
  41. Castiglioni A, Hettmer S, Lynes M, Rao T, Tchessalova D, Sinha I, et al. Isolation of Progenitors that Exhibit Myogenic/Osteogenic Bipotency In Vitro by Fluorescence-Activated Cell Sorting from Human Fetal Muscle. Stem Cell Reports. 2014;2:92-106 pubmed publisher
    ..expressing the satellite cell marker PAX7 are highly enriched within the subset of CD45-CD11b-GlyA-CD31-CD34-CD56intITGA7hi hMFA cells...
  42. Zou Y, Chen T, Feng L, Zhang S, Xing D, Wang Z. Enhancement of 5-aminolevulinic acid production by metabolic engineering of the glycine biosynthesis pathway in Corynebacterium glutamicum. Biotechnol Lett. 2017;39:1369-1374 pubmed publisher
    ..glutamicumAP2, leading to an increase of 70% in 5-ALA production. Further overexpression of the glyA gene increased production of 5-ALA by 150% over the control...
  43. Palmer A, Kamynina E, Field M, Stover P. Folate rescues vitamin B12 depletion-induced inhibition of nuclear thymidylate biosynthesis and genome instability. Proc Natl Acad Sci U S A. 2017;114:E4095-E4102 pubmed publisher
    ..These studies also revealed that 5-formylTHF, a slow, tight-binding inhibitor of serine hydroxymethyltransferase (SHMT), was enriched in nuclei, accounting for 35% of folate cofactors, explaining previous observations that nuclear ..
  44. DeNicola G, Chen P, Mullarky E, Sudderth J, Hu Z, Wu D, et al. NRF2 regulates serine biosynthesis in non-small cell lung cancer. Nat Genet. 2015;47:1475-81 pubmed publisher
    ..We found that NRF2 controls the expression of the key serine/glycine biosynthesis enzyme genes PHGDH, PSAT1 and SHMT2 via ATF4 to support glutathione and nucleotide production...
  45. Najer A, Wu D, Nussbaumer M, Schwertz G, Schwab A, Witschel M, et al. An amphiphilic graft copolymer-based nanoparticle platform for reduction-responsive anticancer and antimalarial drug delivery. Nanoscale. 2016;8:14858-69 pubmed publisher
    ..HeLa cells) and an experimental, metabolically unstable antimalarial drug (the serine hydroxymethyltransferase (SHMT) inhibitor (±)-1) to Plasmodium falciparum-infected red blood cells (iRBCs), with higher efficacy compared to ..
  46. Kamynina E, Lachenauer E, DiRisio A, Liebenthal R, Field M, Stover P. Arsenic trioxide targets MTHFD1 and SUMO-dependent nuclear de novo thymidylate biosynthesis. Proc Natl Acad Sci U S A. 2017;114:E2319-E2326 pubmed publisher
    ..Methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) and serine hydroxymethyltransferase (SHMT) generate 5,10-methylenetetrahydrofolate for de novo dTMP biosynthesis and translocate to the nucleus during S-..
  47. Zwart S, Gregory J, Zeisel S, Gibson C, Mader T, Kinchen J, et al. Genotype, B-vitamin status, and androgens affect spaceflight-induced ophthalmic changes. FASEB J. 2016;30:141-8 pubmed publisher
    ..methionine synthase reductase (MTRR), methylenetetrahydrofolate reductase (MTHFR), serine hydroxymethyltransferase (SHMT), and cystathionine β-synthase (CBS) genes and their association with ophthalmic changes after flight in 49 ..
  48. Mundhada H, Schneider K, Christensen H, Nielsen A. Engineering of high yield production of L-serine in Escherichia coli. Biotechnol Bioeng. 2016;113:807-16 pubmed publisher
    ..of three L-serine deaminases sdaA, sdaB, and tdcG, as well as serine hydroxyl methyl transferase (SHMT) encoded by glyA. Upon overexpression of the serine production pathway, consisting of a feedback resistant version of serA along ..
  49. Ernst D, Downs D. 2-Aminoacrylate Stress Induces a Context-Dependent Glycine Requirement in ridA Strains of Salmonella enterica. J Bacteriol. 2016;198:536-43 pubmed publisher
    ..Salmonella enterica led to the inactivation of multiple target enzymes, including serine hydroxymethyltransferase (GlyA)...
  50. Lyu P, Zhang S, Yuen H, McCrudden C, Wen Q, Chan K, et al. Identification of TWIST-interacting genes in prostate cancer. Sci China Life Sci. 2017;60:386-396 pubmed publisher
    ..in the prostate cancer cohort revealed that gene expression of SERPINA, STX7, PDIA2, FMP5, GP1BB, VGLL4, KCNMA1, SHMT2, SAA4 and DIDO1 influence the prognostic significance of TWIST and vice versa...
  51. Munoz M, Ríos Chaparro D, Patarroyo M, Ramírez J. Determining Clostridium difficile intra-taxa diversity by mining multilocus sequence typing databases. BMC Microbiol. 2017;17:62 pubmed publisher
    ..conserved genes allowing a limited amount of clusters to be discriminated; however, different genes such as drx, glyA and particularly sodA showed high diversity indexes and grouped CD populations in many clusters, suggesting that ..
  52. Koseki J, Konno M, Asai A, Colvin H, Kawamoto K, Nishida N, et al. Enzymes of the one-carbon folate metabolism as anticancer targets predicted by survival rate analysis. Sci Rep. 2018;8:303 pubmed publisher
    ..Patients with high expression of genes that control the metabolic cycle of tetrahydrofolate (THF) in mitochondria, SHMT2, MTHFD2, and ALDH1L2, have a shorter overall survival rate compared with patients with low expression of these ..
  53. Zhang M, Wu W, Chen Z. Structure and function of cytoplasmic serine hydroxymethyltransferase from Pichia pastoris. Biochem Biophys Res Commun. 2018;496:753-757 pubmed publisher
    Serine hydroxymethyltransferase (SHMT) catalyzes the interconversion of serine and glycine, which is crucial for one carbon metabolism...
  54. Roy G, Ouellette M. Inactivation of the cytosolic and mitochondrial serine hydroxymethyl transferase genes in Leishmania major. Mol Biochem Parasitol. 2015;204:106-110 pubmed publisher
    Leishmania has two serine hydroxylmethyl transferase (SHMT) genes, one coding for a cytosolic and the other for a mitochondrial enzyme. Trypanosoma cruzi has only the gene coding for the cytosolic enzyme and Trypanosoma brucei has no SHMT...
  55. Elbrissi A, Sabeil Y, Khalifa K, Enan K, Khair O, El Hussein A. Isolation, identification and differentiation of Campylobacter spp. using multiplex PCR assay from goats in Khartoum State, Sudan. Trop Anim Health Prod. 2017;49:575-581 pubmed publisher
    ..The genes selected were hipO (hippuricase) and 23S rRNA from glyA (serine hydroxymethyl transferase) from each of C. jejuni. C. coli, C. lari, and C...
  56. Celardo I, Lehmann S, Costa A, Loh S, Miguel Martins L. dATF4 regulation of mitochondrial folate-mediated one-carbon metabolism is neuroprotective. Cell Death Differ. 2017;24:638-648 pubmed publisher
    ..We show that ATF4 regulates the expression of one-carbon metabolism genes SHMT2 and NMDMC as a protective response to mitochondrial toxicity...
  57. Kałużna M, Kuras A, Puławska J. Validation of reference genes for the normalization of the RT-qPCR gene expression of virulence genes of Erwinia amylovora in apple shoots. Sci Rep. 2017;7:2034 pubmed publisher
    To study the expression of pathogenicity-related genes in Erwinia amylovora, seven candidate reference genes (ffh, glyA, gyrA, proC, pykA, recA, rpoB) were selected and validated with the following five different mathematic algorithms: ..
  58. Oda Y, Fujita Y, Oishi K, Nakata Y, Takase M, Niitsu T, et al. Alterations in glutamatergic signaling in the brain of dopamine supersensitivity psychosis and non-supersensitivity psychosis model rats. Psychopharmacology (Berl). 2017;234:3027-3036 pubmed publisher
    ..In addition, the ratio of L-serine to glycine was increased. The striatal expressions of GAD and SHMT2 in the DSP rats were significantly increased...
  59. Yin K. Positive correlation between expression level of mitochondrial serine hydroxymethyltransferase and breast cancer grade. Onco Targets Ther. 2015;8:1069-74 pubmed publisher
    ..Although the mitochondrial isoform of SHMT (SHMT2) has been proven to be a crucial factor in the serine/glycine metabolism in several cancer cell types, the ..
  60. Lee J, Chae J, Kim J, Kim H, Chung J, Kim Y. Inhibition of cisplatin-resistance by RNA interference targeting metallothionein using reducible oligo-peptoplex. J Control Release. 2015;215:82-90 pubmed publisher
    ..The short hairpin RNA targeting MT (shMT) was tested to down-regulate MT and recover cisplatin resistance...
  61. Lin Z, Zhang Y, Yuan Q, Liu Q, Li Y, Wang Z, et al. Metabolic engineering of Escherichia coli for poly(3-hydroxybutyrate) production via threonine bypass. Microb Cell Fact. 2015;14:185 pubmed publisher
    ..23-fold improvement of PHB titer. Then, we overexpressed glyA to enhance the conversion of glycine to serine and activated transhydrogenase to generate NADPH required in the ..
  62. Yuan C, Wang Y, Zhao H, Zhang L, Wang Y, Liu X, et al. Genetic diversity of rhg1 and Rhg4 loci in wild soybeans resistant to soybean cyst nematode race 3. Genet Mol Res. 2016;15: pubmed publisher
    ..soja accessions that are resistant to SCN race 3 for polymorphisms in the resistance genes, rhg1, Rhg4, and SHMT, and investigated their genetic relationship with eight Glycine max resistant cultivars...
  63. Wang B, Wang W, Zhu Z, Zhang X, Tang F, Wang D, et al. Mitochondrial serine hydroxymethyltransferase 2 is a potential diagnostic and prognostic biomarker for human glioma. Clin Neurol Neurosurg. 2017;154:28-33 pubmed publisher
    ..Recently, researchers have discovered that mitochondrial serine hydroxymethyltransferase 2 (SHMT2) is overexpressed in various types of cancer. However, the function of SHMT2 in glioma is not clear...
  64. Sun W, Kim H, Jung W, Koo J. Expression of serine/glycine metabolism-related proteins is different according to the thyroid cancer subtype. J Transl Med. 2016;14:168 pubmed publisher
    ..PHGDH), phosphoserine aminotransferase, (PSAT), phosphoserine phosphatase (PSPH), serine hydromethyl transferase (SHMT), and glycine decarboxylase (GLDC) was performed with the TMA blocks and the results were analyzed together with ..
  65. Wu X, Xu W, Zhou T, Cao N, Ni J, Zou T, et al. The Role of Genetic Polymorphisms as Related to One-Carbon Metabolism, Vitamin B6, and Gene-Nutrient Interactions in Maintaining Genomic Stability and Cell Viability in Chinese Breast Cancer Patients. Int J Mol Sci. 2016;17: pubmed publisher
    ..B6 (B6) is a cofactor, and genetic polymorphisms of related key enzymes, such as serine hydroxymethyltransferase (SHMT), methionine synthase reductase (MTRR), and methionine synthase (MS), in FMOCM may govern the bioavailability of ..
  66. Liu G, Zhang M, Mo T, He L, Zhang W, Yu Y, et al. Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus. Biochem Biophys Res Commun. 2015;467:1058-62 pubmed publisher
    ..paucihalophilus involves different pathways including SHMT, threonine aldolase (TA) and the reverse reaction of glycine cleavage system (GCS), demonstrating for the first time that other ..
  67. Mishra P, Mishra V, Takabe T, Rai V, Singh N. Elucidation of salt-tolerance metabolic pathways in contrasting rice genotypes and their segregating progenies. Plant Cell Rep. 2016;35:1273-86 pubmed publisher
    ..methionine synthase (SAMS), S-adenosyl methionine decarboxylase (SAMDC) and serine hydroxymethyl transferase (SHMT) genes in tolerant lines, suggesting potential role of the MBP in conferring salt tolerance in rice variety CSR27...
  68. Mirgal D, Ghosh K, Mahanta J, Dutta P, Shetty S. Possible selection of host folate pathway gene polymorphisms in patients with malaria from a malaria endemic region in North East India. Trans R Soc Trop Med Hyg. 2016;110:294-8 pubmed publisher
    ..cystathionine beta-synthase (CBS) 844ins68, reduced folate carrier-1 (RFC-1) G80A, serine hydroxymethyltransferase (SHMT) C1420T, methionine synthase (MTR) A2756G, MTHFR G1793A (D 919G), glycine N-methyltransferase (GNMT) 1289 by PCR-..
  69. Jiang Y, Jin H, Yang H, Zhao R, Wang S, Chen Y, et al. Defining the enzymatic pathway for polymorphic O-glycosylation of the pneumococcal serine-rich repeat protein PsrP. J Biol Chem. 2017;292:6213-6224 pubmed publisher
    ..the full-length GlyE and GlyG proteins and the GlyD DUF1792 domain participate in both steps, whereas full-length GlyA and the GlyD GT8 domain catalyze only the fourth step...
  70. Tani H, Ohnishi S, Shitara H, Mito T, Yamaguchi M, Yonekawa H, et al. Mice deficient in the Shmt2 gene have mitochondrial respiration defects and are embryonic lethal. Sci Rep. 2018;8:425 pubmed publisher
    ..g., glycine C-acetyltransferase (GCAT), serine hydroxymethyltransferase 2 (SHMT2)] is related to age-associated respiration defects...
  71. Brown R, Holden M, Spiller O, Chalker V. Development of a Multilocus Sequence Typing Scheme for Molecular Typing of Mycoplasma pneumoniae. J Clin Microbiol. 2015;53:3195-203 pubmed publisher
    ..pneumoniae was developed based on the sequences of eight housekeeping genes (ppa, pgm, gyrB, gmk, glyA, atpA, arcC, and adk) and applied to 55 M. pneumoniae clinical isolates and the two type strains M129 and FH...
  72. Kumudini N, Naushad S, Alex Stanley B, Niveditha M, Sharmila G, Kumaraswami K, et al. Comparative analysis of four disease prediction models of Parkinson's disease. Mol Cell Biochem. 2016;411:127-34 pubmed publisher
    ..The RP model showed SHMT C1420T as important determinant of PD risk...
  73. Ron Harel N, Santos D, Ghergurovich J, Sage P, Reddy A, Lovitch S, et al. Mitochondrial Biogenesis and Proteome Remodeling Promote One-Carbon Metabolism for T Cell Activation. Cell Metab. 2016;24:104-17 pubmed publisher
    ..Genetic inhibition of the mitochondrial serine catabolic enzyme SHMT2 impaired T cell survival in culture and antigen-specific T cell abundance in vivo...
  74. Misselbeck K, Marchetti L, Field M, Scotti M, Priami C, Stover P. A hybrid stochastic model of folate-mediated one-carbon metabolism: Effect of the common C677T MTHFR variant on de novo thymidylate biosynthesis. Sci Rep. 2017;7:797 pubmed publisher
    ..neural tube defects (NTDs) indicate that impaired de novo thymidylate (dTMP) synthesis through changes in SHMT expression is causative in folate-responsive NTDs...
  75. Lizcano A, Akula Suresh Babu R, Shenoy A, Saville A, Kumar N, D Mello A, et al. Transcriptional organization of pneumococcal psrP-secY2A2 and impact of GtfA and GtfB deletion on PsrP-associated virulence properties. Microbes Infect. 2017;19:323-333 pubmed publisher
    ..PCR amplification of cDNA and RNA-seq analysis found psrP-secY2A2 to be minimally composed of three operons: psrP-glyA, glyB, and glyC-asp5...
  76. Massari F, Ciccarese C, Santoni M, Iacovelli R, Mazzucchelli R, Piva F, et al. Metabolic phenotype of bladder cancer. Cancer Treat Rev. 2016;45:46-57 pubmed publisher
    ..Moreover, AGL loss determines augmented levels of the serine-to-glycine enzyme serine hydroxymethyltransferase-2 (SHMT2), resulting in an increased glycine and purine ring of nucleotides synthesis, thus supporting cells proliferation...
  77. Calise S, Purich D, Nguyen T, Saleem D, Krueger C, Yin J, et al. 'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway. J Cell Sci. 2016;129:3042-52 pubmed publisher
    ..and we have traced these effects to dihydrofolate reductase (DHFR) and serine hydroxymethyltransferase-2 (SHMT2), pivotal enzymes in one-carbon metabolism and nucleotide biosynthesis...
  78. Zhang L, Chen Z, Xue D, Zhang Q, Liu X, Luh F, et al. Prognostic and therapeutic value of mitochondrial serine hydroxyl-methyltransferase 2 as a breast cancer biomarker. Oncol Rep. 2016;36:2489-2500 pubmed publisher
    Mitochondrial serine hydroxylmethyltransferase 2 (SHMT2) is a key enzyme in the serine/glycine synthesis pathway. SHMT2 has been implicated as a critical component for tumor cell survival...
  79. Kandoth P, Liu S, Prenger E, Ludwig A, Lakhssassi N, Heinz R, et al. Systematic Mutagenesis of Serine Hydroxymethyltransferase Reveals an Essential Role in Nematode Resistance. Plant Physiol. 2017;175:1370-1380 pubmed publisher
    ..Mapping of the identified mutations onto the SHMT structural model uncovered key residues for structural stability, ligand binding, enzyme activity, and protein ..
  80. Bernhardt S, Bayerlová M, Vetter M, Wachter A, Mitra D, Hanf V, et al. Proteomic profiling of breast cancer metabolism identifies SHMT2 and ASCT2 as prognostic factors. Breast Cancer Res. 2017;19:112 pubmed publisher
    ..of individual protein expression profiles demonstrated the central role of serine hydroxymethyltransferase 2 (SHMT2) and amino acid transporter ASCT2 (SLC1A5) as independent prognostic factors in breast cancer patients...
  81. Minton D, Nam M, McLaughlin D, Shin J, Bayraktar E, Alvarez S, et al. Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs. Mol Cell. 2018;69:610-621.e5 pubmed publisher
    ..Using genetic screens, we find that the mitochondrial serine hydroxymethyltransferase (SHMT2) is required for robust mitochondrial oxygen consumption and low glucose proliferation...
  82. Trakatellis A, Dimitriadou A, Trakatelli M. Pyridoxine deficiency: new approaches in immunosuppression and chemotherapy. Postgrad Med J. 1997;73:617-22 pubmed
  83. Heil S, van der Put N, Waas E, Den Heijer M, Trijbels F, Blom H. Is mutated serine hydroxymethyltransferase (SHMT) involved in the etiology of neural tube defects?. Mol Genet Metab. 2001;73:164-72 pubmed
    ..Serine hydroxymethyltransferase (SHMT) catalyzes the reversible reaction of serine and tetrahydrofolate (THF) to glycine and 5,10-methylene THF...
  84. Meyer K, Fredriksen A, Ueland P. MALDI-TOF MS genotyping of polymorphisms related to 1-carbon metabolism using common and mass-modified terminators. Clin Chem. 2009;55:139-49 pubmed publisher
    ..This was demonstrated with 20 polymorphisms involved in 1-carbon metabolism. ..
  85. Hebbring S, Chai Y, Ji Y, Abo R, Jenkins G, Fridley B, et al. Serine hydroxymethyltransferase 1 and 2: gene sequence variation and functional genomic characterization. J Neurochem. 2012;120:881-90 pubmed publisher
    ..We set out to resequence SHMT1 and SHMT2, followed by functional genomic studies. We identified 87 and 60 polymorphisms in SHMT1 and SHMT2, respectively...
  86. Singh R, Avliyakulov N, Braga M, Haykinson M, Martinez L, Singh V, et al. Proteomic identification of mitochondrial targets of arginase in human breast cancer. PLoS ONE. 2013;8:e79242 pubmed publisher
    ..As mSHMT is a key player in folate metabolism, our data provides a novel link between arginine and folate metabolism in human breast cancer, both of which are critical for tumor cell proliferation. ..
  87. Giardina G, Brunotti P, Fiascarelli A, Cicalini A, Costa M, Buckle A, et al. How pyridoxal 5'-phosphate differentially regulates human cytosolic and mitochondrial serine hydroxymethyltransferase oligomeric state. FEBS J. 2015;282:1225-41 pubmed publisher
    ..In humans, the cytosolic isoform (SHMT1) and the mitochondrial isoform (SHMT2) have distinct cellular roles, but high sequence identity and comparable catalytic properties, which may ..
  88. Wu X, Deng L, Tang D, Ying G, Yao X, Liu F, et al. miR-615-5p prevents proliferation and migration through negatively regulating serine hydromethyltransferase 2 (SHMT2) in hepatocellular carcinoma. Tumour Biol. 2016;37:6813-21 pubmed publisher
    ..were employed to ascertain the downstream target of miR-615-5p finding that the serine hydromethyltransferase 2 (SHMT2) was the direct downstream target...
  89. Woo C, Chen W, Teo X, Radda G, Lee P. Downregulating serine hydroxymethyltransferase 2 (SHMT2) suppresses tumorigenesis in human hepatocellular carcinoma. Oncotarget. 2016;7:53005-53017 pubmed publisher
    ..Increased de novo biosynthesis of glycine by serine hydroxymethyltransferase 2 (SHMT2) is the central mechanism to fuel one-carbon pools supporting tumorigenesis...
  90. Aljaafri W, McNeece B, Lawaju B, Sharma K, Niruala P, Pant S, et al. A harpin elicitor induces the expression of a coiled-coil nucleotide binding leucine rich repeat (CC-NB-LRR) defense signaling gene and others functioning during defense to parasitic nematodes. Plant Physiol Biochem. 2017;121:161-175 pubmed publisher
    ..alpha soluble N-ethylmaleimide-sensitive fusion protein (?-SNAP) and serine hydroxymethyltransferase (SHMT). In contrast, other defense genes are not directly transcriptionally activated by harpin...
  91. Yang X, Wang Z, Li X, Liu B, Liu M, Liu L, et al. SHMT2 Desuccinylation by SIRT5 Drives Cancer Cell Proliferation. Cancer Res. 2018;78:372-386 pubmed publisher
    The mitochondrial serine hydroxymethyltransferase SHMT2, which catalyzes the rate-limiting step in serine catabolism, drives cancer cell proliferation, but how this role is regulated is undefined...
  92. Bennett F, Jackson A. Glycine is not formed through the amino transferase reaction in human or rat placenta. Placenta. 1998;19:329-31 pubmed