SF3A2

Summary

Gene Symbol: SF3A2
Description: splicing factor 3a subunit 2
Alias: PRP11, PRPF11, SAP62, SF3a66, splicing factor 3A subunit 2, SAP 62, pre-mRNA splicing factor SF3A, subunit 2, spliceosome associated protein 62, splicing factor 3a, subunit 2, 66kD, splicing factor 3a, subunit 2, 66kDa
Species: human
Products:     SF3A2

Top Publications

  1. Yan D, Perriman R, Igel H, Howe K, Neville M, Ares M. CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif. Mol Cell Biol. 1998;18:5000-9 pubmed
    ..Anti-Tat-SF1 antibodies coimmunoprecipitate SF3a66 (SAP62), the human homolog of PRP11, suggesting that Tat-SF1 has a parallel function in splicing in human cells.
  2. Khalid M, Idichi T, Seki N, Wada M, Yamada Y, Fukuhisa H, et al. Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation. Cancers (Basel). 2019;11: pubmed publisher
    ..Among these target genes, high expression levels of RACGAP1, DHRS9, AP1S3, FOXC1, PRP11, RHBDL2 and MUC4 were significant predictors of a poor prognosis of patients with PDAC...
  3. Lin P, Xu R. Structure and assembly of the SF3a splicing factor complex of U2 snRNP. EMBO J. 2012;31:1579-90 pubmed publisher
    ..The structure shows a bifurcated assembly of three subunits, Prp9, Prp11 and Prp21, with Prp9 interacting with Prp21 via a bidentate-binding mode, and Prp21 wrapping around Prp11...
  4. Yan C, Wan R, Bai R, Huang G, Shi Y. Structure of a yeast activated spliceosome at 3.5 Å resolution. Science. 2016;353:904-11 pubmed publisher
    ..catalytic latency is maintained by the SF3b complex, which encircles the BPS, and the splicing factors Cwc24 and Prp11, which shield the 5' exon-5'SS junction...
  5. Schneider C, Agafonov D, Schmitzová J, Hartmuth K, Fabrizio P, Luhrmann R. Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes. PLoS Genet. 2015;11:e1005539 pubmed publisher
    ..Contacts between nucleotides upstream and downstream of the branch-site and the U2 SF3a/b proteins Prp9, Prp11, Hsh49, Cus1 and Hsh155 were detected, demonstrating that these interactions are evolutionarily conserved...
  6. Rigo N, Sun C, Fabrizio P, Kastner B, Lührmann R. Protein localisation by electron microscopy reveals the architecture of the yeast spliceosomal B complex. EMBO J. 2015;34:3059-73 pubmed publisher
    ..We found several U2 SF3a (Prp9 and Prp11) and SF3b (Hsh155 and Cus1) proteins in the head domain and two U4/U6 snRNP proteins (Prp3 and Lsm4) in the neck ..
  7. Rain J, Tartakoff A, Kramer A, Legrain P. Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution. RNA. 1996;2:535-50 pubmed
    ..They do not include the most conserved surp1 module, suggesting that the conservation of this motif in two families of proteins may reflect a still unknown function dispensable in yeast under standard conditions. ..
  8. Takenaka K, Nakagawa H, Miyamoto S, Miki H. The pre-mRNA-splicing factor SF3a66 functions as a microtubule-binding and -bundling protein. Biochem J. 2004;382:223-30 pubmed
    ..This complex consists of three subunits: SF3a60, SF3a66 and SF3a120. Here, we report a possible non-canonical function of a well-characterized RNA-splicing factor, SF3a66...
  9. Yoshimoto R, Okawa K, Yoshida M, Ohno M, Kataoka N. Identification of a novel component C2ORF3 in the lariat-intron complex: lack of C2ORF3 interferes with pre-mRNA splicing via intron turnover pathway. Genes Cells. 2014;19:78-87 pubmed publisher
    ..Interestingly, C2ORF3 protein is localized in both the nucleoplasm and nucleoli, which suggests a potential function in rRNA processing. ..

More Information

Publications23

  1. Dresser D, Jamin S, Atkins C, Guerrier D. An expressed GNRP-like gene shares a bi-directional promoter with SF3A2 (SAP62) immediately upstream of AMH. Gene. 2001;277:163-73 pubmed
    A region of homology, containing the contiguous SF3A2 (formerly called SAP62) and AMH genes, exists between human chromosome 19 (HSA19p) and mouse chromosome 10 (MMU10)...
  2. Will C, Schneider C, MacMillan A, Katopodis N, Neubauer G, Wilm M, et al. A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site. EMBO J. 2001;20:4536-46 pubmed
    ..Immuno precipitations indicated that p14 is present in U12-type spliceosomes, consistent with the idea that branch point selection is similar in the major and minor spliceosomes. ..
  3. Hong W, Bennett M, Xiao Y, Feld Kramer R, Wang C, Reed R. Association of U2 snRNP with the spliceosomal complex E. Nucleic Acids Res. 1997;25:354-61 pubmed
    ..In this study, we have generated and characterized a monoclonal antibody (mAb 4G8) directed against SAP 62, a component of U2 snRNP and a subunit of the essential mammalian splicing factor SF3a...
  4. Dresser D, Hacker A, Lovell Badge R, Guerrier D. The genes for a spliceosome protein (SAP62) and the anti-Müllerian hormone (AMH) are contiguous. Hum Mol Genet. 1995;4:1613-8 pubmed
    ..hormone (Amh) gene, we identified a region of homology with the known cDNA sequence of a human spliceosome gene (SAP62)...
  5. Nesic D, Kramer A. Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation. Mol Cell Biol. 2001;21:6406-17 pubmed
    ..We have analyzed the function of individual subunits of human SF3a (SF3a60, SF3a66, and SF3a120) by testing recombinant proteins, expressed in insect cells, in various in vitro assays...
  6. Nesic D, Tanackovic G, Kramer A. A role for Cajal bodies in the final steps of U2 snRNP biogenesis. J Cell Sci. 2004;117:4423-33 pubmed
    ..To analyze cellular aspects of this process, we studied domains in SF3a60 and SF3a66 that are required for their localization to nuclear speckles...
  7. Tanackovic G, Kramer A. Human splicing factor SF3a, but not SF1, is essential for pre-mRNA splicing in vivo. Mol Biol Cell. 2005;16:1366-77 pubmed
    ..Together with the observation that the 17S U2 snRNP cannot be detected in extracts from SF3a-depleted cells, our results provide further evidence for a function of Cajal bodies in U2 snRNP biogenesis. ..
  8. Llères D, Denegri M, Biggiogera M, Ajuh P, Lamond A. Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice. EMBO Rep. 2010;11:445-51 pubmed publisher
    ..hnRNP-M affects both 5' and 3' alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate. ..
  9. Hirokawa M, Morita H, Tajima T, Takahashi A, Ashikawa K, Miya F, et al. A genome-wide association study identifies PLCL2 and AP3D1-DOT1L-SF3A2 as new susceptibility loci for myocardial infarction in Japanese. Eur J Hum Genet. 2015;23:374-80 pubmed publisher
    ..3 (rs4618210:A>G, P = 2.60 × 10(-9), odds ratio (OR) = 0.91) and AP3D1-DOT1L-SF3A2 on chromosome 19p13.3 (rs3803915:A>C, P = 3.84 × 10(-9), OR = 0.89)...
  10. Crisci A, Raleff F, Bagdiul I, Raabe M, Urlaub H, Rain J, et al. Mammalian splicing factor SF1 interacts with SURP domains of U2 snRNP-associated proteins. Nucleic Acids Res. 2015;43:10456-73 pubmed publisher
    ..In addition, these findings may have implications for alternative splicing decisions. ..
  11. Moraitou M, Patrinou Georgoula M, Guialis A. Structural/functional properties of a mammalian multi-component structure containing all major spliceosomal small nuclear ribonucleoprotein particles. Biochem J. 1998;332 ( Pt 1):135-44 pubmed
    ..Moreover, these fractions were capable of restoring splicing activity when applied in reconstitution studies to supplement a micrococcal nuclease-treated splicing extract. ..
  12. Bennett M, Reed R. Correspondence between a mammalian spliceosome component and an essential yeast splicing factor. Science. 1993;262:105-8 pubmed
    ..strategy was used to isolate a complementary DNA encoding the mammalian spliceosome-associated protein (SAP) SAP 62. It is demonstrated that SAP 62 is the likely functional homolog of the yeast PRP11 protein...
  13. Das R, Zhou Z, Reed R. Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. Mol Cell. 2000;5:779-87 pubmed
    ..These data suggest a model for spliceosome assembly in which U1 and U2 snRNPs first associate with the spliceosome in the E complex and then an ATP-dependent step results in highly stable U2 snRNP binding to the BPS in the A complex. ..
  14. Kramer A, Grüter P, Gröning K, Kastner B. Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP. J Cell Biol. 1999;145:1355-68 pubmed
    ..Comparison to the two-domain structure of the 17S U2 snRNP corroborates the biochemical results in that binding of SF3a contributes to an increase in size of the 12S U2 domain and possibly induces a structural change in the SF3b domain. ..