Gene Symbol: SF3A1
Description: splicing factor 3a subunit 1
Alias: PRP21, PRPF21, SAP114, SF3A120, splicing factor 3A subunit 1, SAP 114, pre-mRNA processing 21, pre-mRNA splicing factor SF3a (120 kDa subunit), spliceosome-associated protein 114, splicing factor 3 subunit 1, splicing factor 3a, subunit 1, 120kD, splicing factor 3a, subunit 1, 120kDa
Species: human
Products:     SF3A1

Top Publications

  1. Huang C, Ferfoglia F, Raleff F, Kramer A. Interaction domains and nuclear targeting signals in subunits of the U2 small nuclear ribonucleoprotein particle-associated splicing factor SF3a. J Biol Chem. 2011;286:13106-14 pubmed publisher
    ..Our results indicate that the SF3a66-SF3a120 interaction is mediated by a 27-amino acid region in SF3a120 C-terminal to the second suppressor-of-white-apricot ..
  2. Kramer A, Mulhauser F, Wersig C, Gröning K, Bilbe G. Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae. RNA. 1995;1:260-72 pubmed
    ..A cDNA encoding the 120-kDa subunit of SF3a has been cloned. The SF3a120 gene was localized to human chromosome 22, and three mRNAs of 3.2, 3.8, and 5.7 kb are ubiquitously expressed...
  3. Nesic D, Kramer A. Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation. Mol Cell Biol. 2001;21:6406-17 pubmed
    ..We have analyzed the function of individual subunits of human SF3a (SF3a60, SF3a66, and SF3a120) by testing recombinant proteins, expressed in insect cells, in various in vitro assays...
  4. Nesic D, Tanackovic G, Kramer A. A role for Cajal bodies in the final steps of U2 snRNP biogenesis. J Cell Sci. 2004;117:4423-33 pubmed
    ..Regions in SF3a60 and SF3a66 that mediate the binding to SF3a120 are necessary for nuclear import of the proteins, suggesting that the SF3a heterotrimer forms in the cytoplasm...
  5. Tanackovic G, Kramer A. Human splicing factor SF3a, but not SF1, is essential for pre-mRNA splicing in vivo. Mol Biol Cell. 2005;16:1366-77 pubmed
    ..Together with the observation that the 17S U2 snRNP cannot be detected in extracts from SF3a-depleted cells, our results provide further evidence for a function of Cajal bodies in U2 snRNP biogenesis. ..
  6. Kramer A, Grüter P, Gröning K, Kastner B. Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP. J Cell Biol. 1999;145:1355-68 pubmed
    ..Comparison to the two-domain structure of the 17S U2 snRNP corroborates the biochemical results in that binding of SF3a contributes to an increase in size of the 12S U2 domain and possibly induces a structural change in the SF3b domain. ..
  7. Aggerholm Pedersen N, Safwat A, Bærentzen S, Nordsmark M, Nielsen O, Alsner J, et al. The importance of reference gene analysis of formalin-fixed, paraffin-embedded samples from sarcoma patients - an often underestimated problem. Transl Oncol. 2014;7:687-93 pubmed publisher
    ..Recommended reference genes differed with histologic types. However, PPIA, SF3A1, and MRPL19 were stably expressed regardless of the histologic type included...
  8. Félix A, Ciudad C, Noe V. Functional pharmacogenomics and toxicity of PolyPurine Reverse Hoogsteen hairpins directed against survivin in human cells. Biochem Pharmacol. 2018;155:8-20 pubmed publisher
    ..within the response of PC3 cells to treatment with the PPRH against survivin, mainly POLR2G, PAK1IP1, SMC3, SF3A1, PPARGC1A, NCOA6, UGT2B7, ALG5, VAMP7 and HIST1H2BE, the former six present in the Gene Sets detected in the GSEA...
  9. O Connor B, Danhorn T, De Arras L, Flatley B, Marcus R, Farias Hesson E, et al. Regulation of toll-like receptor signaling by the SF3a mRNA splicing complex. PLoS Genet. 2015;11:e1004932 pubmed publisher
    ..Here we inhibit SF3a1 using RNAi and subsequently perform an RNAseq study to identify the full complement of genes and splicing events ..

More Information


  1. Pozzi B, Bragado L, Will C, Mammi P, Risso G, Urlaub H, et al. SUMO conjugation to spliceosomal proteins is required for efficient pre-mRNA splicing. Nucleic Acids Res. 2017;45:6729-6745 pubmed publisher
    ..with U4/U6 snRNP components, is unable to co-precipitate U2 and U5 snRNA and the spliceosomal proteins U2-SF3a120 and U5-Snu114...
  2. Thutkawkorapin J, Picelli S, Kontham V, Liu T, Nilsson D, Lindblom A. Exome sequencing in one family with gastric- and rectal cancer. BMC Genet. 2016;17:41 pubmed publisher
    ..were found in 12 different genes; DZIP1L, PCOLCE2, IGSF10, SUCNR1, OR13C8, EPB41L4B, SEC16A, NOTCH1, TAS2R7, SF3A1, GAL3ST1, and TRIOBP. None of the mutations was suggested as a high penetrant mutation...
  3. Huang R, Gorman K, Vinci C, Dobrovetsky E, Gräslund S, Kay B. Streamlining the Pipeline for Generation of Recombinant Affinity Reagents by Integrating the Affinity Maturation Step. Int J Mol Sci. 2015;16:23587-603 pubmed publisher
    ..COP9 signalosome complex subunit 5 (COPS5), mitogen-activated protein kinase kinase 5 (MAP2K5), Splicing factor 3A subunit 1 (SF3A1) and ubiquitin carboxyl-terminal hydrolase 11 (USP11)...
  4. Song W, Zhu B, Tian Y, Zhong R, Tian J, Miao X, et al. [Research on the association between U2-dependent spliceosome gene and hepatocellular cancer]. Zhonghua Liu Xing Bing Xue Za Zhi. 2015;36:634-8 pubmed
    ..The rs5994293 in SF3A1 gene showed a significant association with HCC in both screened population and combined population...
  5. Chiara M, Champion Arnaud P, Buvoli M, Nadal Ginard B, Reed R. Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114. Proc Natl Acad Sci U S A. 1994;91:6403-7 pubmed
    ..essential heterotrimeric splicing factor SF3a, and products of yeast pre-mRNA processing genes PRP9, PRP11, and PRP21 are their likely yeast counterparts...
  6. Watrin E, Demidova M, Watrin T, Hu Z, Prigent C. Sororin pre-mRNA splicing is required for proper sister chromatid cohesion in human cells. EMBO Rep. 2014;15:948-55 pubmed publisher
    ..Inactivation of splicing factors SF3a120 and U2AF65 induces similar cohesion defects to Prp19 complex inactivation...
  7. De Arras L, Alper S. Limiting of the innate immune response by SF3A-dependent control of MyD88 alternative mRNA splicing. PLoS Genet. 2013;9:e1003855 pubmed publisher
    ..Thus, the SF3A mRNA splicing complex controls production of a negative regulator of TLR signaling that limits the extent of innate immune activation. ..
  8. Pabis M, Neufeld N, Steiner M, Bojic T, Shav Tal Y, Neugebauer K. The nuclear cap-binding complex interacts with the U4/U6·U5 tri-snRNP and promotes spliceosome assembly in mammalian cells. RNA. 2013;19:1054-63 pubmed publisher
    ..Taken together, the data support a model whereby CBC promotes pre-mRNA splicing through a network of interactions with and among spliceosomal snRNPs during cotranscriptional spliceosome assembly. ..
  9. Grimsby S, Jaensson H, Dubrovska A, Lomnytska M, Hellman U, Souchelnytskyi S. Proteomics-based identification of proteins interacting with Smad3: SREBP-2 forms a complex with Smad3 and inhibits its transcriptional activity. FEBS Lett. 2004;577:93-100 pubmed
    ..We found that SREBP-2 inhibited the transcriptional activity of Smad3 in luciferase reporter assays. ..
  10. Will C, Schneider C, MacMillan A, Katopodis N, Neubauer G, Wilm M, et al. A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site. EMBO J. 2001;20:4536-46 pubmed
    ..Immuno precipitations indicated that p14 is present in U12-type spliceosomes, consistent with the idea that branch point selection is similar in the major and minor spliceosomes. ..
  11. Das R, Zhou Z, Reed R. Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. Mol Cell. 2000;5:779-87 pubmed
    ..These data suggest a model for spliceosome assembly in which U1 and U2 snRNPs first associate with the spliceosome in the E complex and then an ATP-dependent step results in highly stable U2 snRNP binding to the BPS in the A complex. ..
  12. Lin P, Xu R. Structure and assembly of the SF3a splicing factor complex of U2 snRNP. EMBO J. 2012;31:1579-90 pubmed publisher
    ..The structure shows a bifurcated assembly of three subunits, Prp9, Prp11 and Prp21, with Prp9 interacting with Prp21 via a bidentate-binding mode, and Prp21 wrapping around Prp11...
  13. Tian J, Liu Y, Zhu B, Tian Y, Zhong R, Chen W, et al. SF3A1 and pancreatic cancer: new evidence for the association of the spliceosome and cancer. Oncotarget. 2015;6:37750-7 pubmed publisher
    ..study was conducted to examine the association between six candidate U2-depedent spliceosome genes (SRSF1, SRSF2, SF3A1, SF3B1, SF1 and PRPF40B) and pancreatic cancer (PC)...
  14. Underhill C, Qutob M, Yee S, Torchia J. A novel nuclear receptor corepressor complex, N-CoR, contains components of the mammalian SWI/SNF complex and the corepressor KAP-1. J Biol Chem. 2000;275:40463-70 pubmed
    ..These results suggest that N-CoR is found in distinct multiprotein complexes, which are involved in multiple pathways of transcriptional repression. ..
  15. Sharma S, Wongpalee S, Vashisht A, Wohlschlegel J, Black D. Stem-loop 4 of U1 snRNA is essential for splicing and interacts with the U2 snRNP-specific SF3A1 protein during spliceosome assembly. Genes Dev. 2014;28:2518-31 pubmed publisher
    ..We show that U1-SL4 interacts with the SF3A1 protein of the U2 snRNP...
  16. Crisci A, Raleff F, Bagdiul I, Raabe M, Urlaub H, Rain J, et al. Mammalian splicing factor SF1 interacts with SURP domains of U2 snRNP-associated proteins. Nucleic Acids Res. 2015;43:10456-73 pubmed publisher
    ..In addition, these findings may have implications for alternative splicing decisions. ..
  17. Gunther M, Laithier M, Brison O. A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening. Mol Cell Biochem. 2000;210:131-42 pubmed
    ..factor were independently selected and two overlapping cDNA clones corresponded to the splicing factor SF3A120. Two different cDNA clones encoded the N- and C-terminal region of the Oct-1 transcription factor...
  18. Rain J, Tartakoff A, Kramer A, Legrain P. Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution. RNA. 1996;2:535-50 pubmed
    ..of Prp21p and to identify potential additional functions of this protein, we isolated a series of heat-sensitive prp21 mutants...
  19. Kuwasako K, He F, Inoue M, Tanaka A, Sugano S, Güntert P, et al. Solution structures of the SURP domains and the subunit-assembly mechanism within the splicing factor SF3a complex in 17S U2 snRNP. Structure. 2006;14:1677-89 pubmed
    The SF3a complex, consisting of SF3a60, SF3a66, and SF3a120, in 17S U2 snRNP is crucial to spliceosomal assembly. SF3a120 contains two tandem SURP domains (SURP1 and SURP2), and SURP2 is responsible for binding to SF3a60...
  20. Chen X, Du H, Liu B, Zou L, Chen W, Yang Y, et al. The Associations between RNA Splicing Complex Gene SF3A1 Polymorphisms and Colorectal Cancer Risk in a Chinese Population. PLoS ONE. 2015;10:e0130377 pubmed publisher
    ..However, the role of SF3A1, one key component of the mRNA splicing machinery, on colorectal cancer (CRC) risk was still not elucidated...