Gene Symbol: SC35
Description: serine and arginine rich splicing factor 2
Alias: PR264, SC-35, SC35, SFRS2, SFRS2A, SRp30b, serine/arginine-rich splicing factor 2, SR splicing factor 2, splicing component, 35 kDa, splicing factor SC35, splicing factor, arginine/serine-rich 2
Species: human
Products:     SC35

Top Publications

  1. Zahler A, Lane W, Stolk J, Roth M. SR proteins: a conserved family of pre-mRNA splicing factors. Genes Dev. 1992;6:837-47 pubmed
    ..In four of the family members a repeated protein sequence that encompasses an RNA recognition motif was observed. We discuss the potential role of this highly conserved, functionally related set of proteins in pre-mRNA splicing. ..
  2. McGarvey T, Rosonina E, McCracken S, Li Q, Arnaout R, Mientjes E, et al. The acute myeloid leukemia-associated protein, DEK, forms a splicing-dependent interaction with exon-product complexes. J Cell Biol. 2000;150:309-20 pubmed
    ..Thus, DEK is a candidate factor for controlling postsplicing steps in gene expression that are influenced by the prior removal of an intron from pre-mRNA. ..
  3. Wang H, Xu X, Ding J, Bermingham J, Fu X. SC35 plays a role in T cell development and alternative splicing of CD45. Mol Cell. 2001;7:331-42 pubmed
    ..Here we report that Cre-mediated conditional deletion of the prototypical SR protein SC35 in the thymus causes a defect in T cell maturation...
  4. Merdzhanova G, Edmond V, De Seranno S, van den Broeck A, Corcos L, Brambilla C, et al. E2F1 controls alternative splicing pattern of genes involved in apoptosis through upregulation of the splicing factor SC35. Cell Death Differ. 2008;15:1815-23 pubmed publisher
    ..In this study, we identify the splicing factor SC35, a member of the Ser-Rich Arg (SR) proteins family, as a new transcriptional target of E2F1...
  5. Fu X, Maniatis T. Isolation of a complementary DNA that encodes the mammalian splicing factor SC35. Science. 1992;256:535-8 pubmed
    The mammalian splicing factor SC35 is required for the first step in the splicing reaction and for spliceosome assembly...
  6. Tacke R, Manley J. The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 1995;14:3540-51 pubmed
    ASF/SF2 and SC35 belong to a highly conserved family of nuclear proteins that are both essential for splicing of pre-mRNA in vitro and are able to influence selection of alternative splice sites...
  7. Kohtz J, Jamison S, Will C, Zuo P, Luhrmann R, Garcia Blanco M, et al. Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors. Nature. 1994;368:119-24 pubmed
    ..This cooperation is probably achieved by specific interactions between the arginine/serine-rich domain of the splicing factor and a similar region in a U1 snRNP-specific protein. ..
  8. Tronchere H, Wang J, Fu X. A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA. Nature. 1997;388:397-400 pubmed
    ..Therefore, Urp and U2AF35 may independently position RS-domain-containing factors within spliceosomes. ..
  9. Yang L, Embree L, Tsai S, Hickstein D. Oncoprotein TLS interacts with serine-arginine proteins involved in RNA splicing. J Biol Chem. 1998;273:27761-4 pubmed
    ..The first SR protein is the mouse homolog of human splicing factor SC35, and the second SR member is a novel 183-amino acid protein that we term TASR (TLS-associated serine-..

More Information

Publications125 found, 100 shown here

  1. Zahler A, Damgaard C, Kjems J, Caputi M. SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing. J Biol Chem. 2004;279:10077-84 pubmed
    ..nuclear ribonucleoprotein (hnRNP) A/B proteins bind the ESS to repress splicing, whereas the SR protein SC35 binds the ESE to activate it...
  2. Ropers D, Ayadi L, Gattoni R, Jacquenet S, Damier L, Branlant C, et al. Differential effects of the SR proteins 9G8, SC35, ASF/SF2, and SRp40 on the utilization of the A1 to A5 splicing sites of HIV-1 RNA. J Biol Chem. 2004;279:29963-73 pubmed
    ..As a result, upon ASF/SF2 overexpression, the vpr mRNA steady state level is specifically increased. SC35 and SRp40, but not 9G8, strongly activate site A3, and their overexpression ex vivo induces a dramatic accumulation ..
  3. Edmond V, Moysan E, Khochbin S, Matthias P, Brambilla C, Brambilla E, et al. Acetylation and phosphorylation of SRSF2 control cell fate decision in response to cisplatin. EMBO J. 2011;30:510-23 pubmed publisher
    ..Taken together, these results unravel lysine acetylation as a crucial post-translational modification regulating SRSF2 protein level and activity in response to genotoxic stress. ..
  4. Makishima H, Visconte V, Sakaguchi H, Jankowska A, Abu Kar S, Jerez A, et al. Mutations in the spliceosome machinery, a novel and ubiquitous pathway in leukemogenesis. Blood. 2012;119:3203-10 pubmed publisher
    ..Spliceosomal genes are probably tumor suppressors, and their mutations may constitute diagnostic biomarkers that could potentially serve as therapeutic targets...
  5. Thol F, Kade S, Schlarmann C, Löffeld P, Morgan M, Krauter J, et al. Frequency and prognostic impact of mutations in SRSF2, U2AF1, and ZRSR2 in patients with myelodysplastic syndromes. Blood. 2012;119:3578-84 pubmed publisher
    ..83; 95% confidence interval, 1.31-6.12; P = .008). These results show a negative prognostic impact of SRSF2 mutations in MDS. SRSF2 mutations may become useful for clinical risk stratification and treatment decisions in the future. ..
  6. Wu S, Kuo Y, Hou H, Li L, Tseng M, Huang C, et al. The clinical implication of SRSF2 mutation in patients with myelodysplastic syndrome and its stability during disease evolution. Blood. 2012;120:3106-11 pubmed publisher
    ..In conclusion, SRSF2 mutation is associated with distinct clinical and biologic features in MDS patients. It is stable during the clinical course and may play little role in disease progression. ..
  7. Lasho T, Jimma T, Finke C, Patnaik M, Hanson C, Ketterling R, et al. SRSF2 mutations in primary myelofibrosis: significant clustering with IDH mutations and independent association with inferior overall and leukemia-free survival. Blood. 2012;120:4168-71 pubmed publisher
    ..01; HR = 1.9; 95% CI, 1.1-3.0) and IDH mutations (P < .01; HR = 2.3; 95% CI, 1.4-3.8). In conclusion, SRSF2 mutations are relatively common in PMF, cluster with IDH mutations, and are independently predictive of poor outcome. ..
  8. Je E, Yoo N, Kim Y, Kim M, Lee S. Mutational analysis of splicing machinery genes SF3B1, U2AF1 and SRSF2 in myelodysplasia and other common tumors. Int J Cancer. 2013;133:260-5 pubmed publisher
    ..The data suggest that the splicing gene mutations play important roles in the pathogenesis of hematologic tumors, but rarely in solid tumors. ..
  9. Wu J, Maniatis T. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing. Cell. 1993;75:1061-70 pubmed
    ..We report that the splicing factors SC35 and SF2/ASF specifically interact with both the integral U1 small nuclear ribonucleoprotein (snRNP U1-70K) and with ..
  10. Fu X. Specific commitment of different pre-mRNAs to splicing by single SR proteins. Nature. 1993;365:82-5 pubmed
    ..These SR proteins, which include SC35 and SF2/ASF, are conserved from Drosophila to man, are required for early steps of spliceosome assembly and can ..
  11. Barnard D, Patton J. Identification and characterization of a novel serine-arginine-rich splicing regulatory protein. Mol Cell Biol. 2000;20:3049-57 pubmed
    ..inhibit the in vitro and in vivo activation of specific splice sites by a subset of SR proteins, including ASF/SF2, SC35, and SRp55. In contrast, p86 activates splicing in the presence of SRp20...
  12. von Mikecz A, Zhang S, Montminy M, Tan E, Hemmerich P. CREB-binding protein (CBP)/p300 and RNA polymerase II colocalize in transcriptionally active domains in the nucleus. J Cell Biol. 2000;150:265-73 pubmed
    ..The identification of pol II in CBP/PML-containing nuclear bodies supports the idea that transcription takes place at PML bodies. ..
  13. Solis A, Peng R, Crawford J, Phillips J, Patton J. Growth hormone deficiency and splicing fidelity: two serine/arginine-rich proteins, ASF/SF2 and SC35, act antagonistically. J Biol Chem. 2008;283:23619-26 pubmed publisher
    ..Here, we show that two members of the serine/arginine-rich (SR) protein superfamily (ASF/SF2 and SC35) act antagonistically to regulate exon 3 splicing...
  14. Chandradas S, Deikus G, Tardos J, Bogdanov V. Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells. J Leukoc Biol. 2010;87:147-52 pubmed publisher
    ..In this report, we show for the first time that two other SR proteins expressed in human monocytes, SRp40 and SC35, antagonize ASF/SF2 and SRp55 by competing for binding to certain sites in exon 5, thereby promoting TF exon 5 ..
  15. Meggendorfer M, Roller A, Haferlach T, Eder C, Dicker F, Grossmann V, et al. SRSF2 mutations in 275 cases with chronic myelomonocytic leukemia (CMML). Blood. 2012;120:3080-8 pubmed publisher
    ..In conclusion, these data show the importance of SRSF2mut as new diagnostic marker in CMML. ..
  16. Patnaik M, Lasho T, Finke C, Hanson C, Hodnefield J, Knudson R, et al. Spliceosome mutations involving SRSF2, SF3B1, and U2AF35 in chronic myelomonocytic leukemia: prevalence, clinical correlates, and prognostic relevance. Am J Hematol. 2013;88:201-6 pubmed publisher
    ..We conclude that SRSF2 is the most frequently mutated spliceosome gene in CMML but neither it nor SF3B1 or U2AF35 mutations are prognostically relevant. ..
  17. Hanssens K, Brenet F, Agopian J, Georgin Lavialle S, Damaj G, Cabaret L, et al. SRSF2-p95 hotspot mutation is highly associated with advanced forms of mastocytosis and mutations in epigenetic regulator genes. Haematologica. 2014;99:830-5 pubmed publisher
    ..This highlights the prognostic potential of such approaches, if applied systematically, for delineating the roles of specific mutations in predisposing and/or driving distinct disease phenotypes. ..
  18. Cruz T, Carvalho R, Richardson D, Duque P. Abscisic acid (ABA) regulation of Arabidopsis SR protein gene expression. Int J Mol Sci. 2014;15:17541-64 pubmed publisher
    ..We found that members of the plant-specific SC35-Like (SCL) Arabidopsis SR protein subfamily are distinctively responsive to exogenous ABA, while the expression of ..
  19. Hallay H, Locker N, Ayadi L, Ropers D, Guittet E, Branlant C. Biochemical and NMR study on the competition between proteins SC35, SRp40, and heterogeneous nuclear ribonucleoprotein A1 at the HIV-1 Tat exon 2 splicing site. J Biol Chem. 2006;281:37159-74 pubmed
    ..This interaction has limited effect on the RNA two-dimensional structure. The SR proteins SC35 and SRp40 were found previously to be strong activators of site A3 utilization...
  20. Cmarko D, Bøe S, Scassellati C, Szilvay A, Davanger S, Fu X, et al. Rev inhibition strongly affects intracellular distribution of human immunodeficiency virus type 1 RNAs. J Virol. 2002;76:10473-84 pubmed
    ..The simultaneous demonstration of Rev, Tat, Br-labeled RNA, and cellular proteins SC35 and CRM1 in such fibrils reveals the potential of Rev to associate with nascent HIV pre-mRNA and its splicing ..
  21. Lu Y, Loh Y, Li H, Cesana M, Ficarro S, Parikh J, et al. Alternative splicing of MBD2 supports self-renewal in human pluripotent stem cells. Cell Stem Cell. 2014;15:92-101 pubmed publisher
    ..We demonstrate that the splicing factor SFRS2 is an OCT4 target gene required for pluripotency...
  22. Yang J, Yao D, Ma J, Yang L, Guo H, Wen X, et al. The prognostic implication of SRSF2 mutations in Chinese patients with acute myeloid leukemia. Tumour Biol. 2016;37:10107-14 pubmed publisher
    ..These results suggest that SRSF2 mutation occurs at a low frequency in aged AML patients and might not be associated with adverse prognosis in Chinese AML patients. ..
  23. An Y, Zou Y, Cao Y, Yao M, Ma N, Wu Y, et al. The nuclear GSK-3β regulated post-transcriptional processing of mRNA through phosphorylation of SC35. Mol Cell Biochem. 2019;451:55-67 pubmed publisher
    ..In this study, we further proved that GSK-3β could specifically co-localize with SC35 in nuclear speckles depending on its kinase activity...
  24. Sureau A, Perbal B. Several mRNAs with variable 3' untranslated regions and different stability encode the human PR264/SC35 splicing factor. Proc Natl Acad Sci U S A. 1994;91:932-6 pubmed
    We have recently established that several mRNAs encode the PR264/SC35 splicing factor and that their expression is related to that of c-Myb in human hematopoietic cells...
  25. Mo S, Ji X, Fu X. Unique role of SRSF2 in transcription activation and diverse functions of the SR and hnRNP proteins in gene expression regulation. Transcription. 2013;4:251-9 pubmed
    ..These findings suggest broad participation of RNA binding proteins in diverse aspects of regulated gene expression at both the transcriptional and posttranscriptional levels in mammalian cells. ..
  26. Erkelenz S, Hillebrand F, Widera M, Theiss S, Fayyaz A, Degrandi D, et al. Balanced splicing at the Tat-specific HIV-1 3'ss A3 is critical for HIV-1 replication. Retrovirology. 2015;12:29 pubmed publisher
  27. Hou W, Cruz Cosme R, Armstrong N, Obwolo L, Wen F, Hu W, et al. Molecular cloning and characterization of the genes encoding the proteins of Zika virus. Gene. 2017;628:117-128 pubmed publisher
    ..early endosome; 6) NS5 forms punctate structures in the nucleus that associate with splicing compartments shown by SC35, leading to reduction of SC35 protein level and trafficking of SC35 from the nucleus to the cytoplasm...
  28. Manley J, Krainer A. A rational nomenclature for serine/arginine-rich protein splicing factors (SR proteins). Genes Dev. 2010;24:1073-4 pubmed publisher
  29. Lindsley R, Mar B, Mazzola E, Grauman P, Shareef S, Allen S, et al. Acute myeloid leukemia ontogeny is defined by distinct somatic mutations. Blood. 2015;125:1367-76 pubmed publisher
    ..This trial was registered at as #NCT00715637. ..
  30. Zhang H, Mahadevan K, Palazzo A. Sumoylation is Required for the Cytoplasmic Accumulation of a Subset of mRNAs. Genes (Basel). 2014;5:982-1000 pubmed publisher
    ..coupled with the overexpression of the intronless mRNAs, caused the redistribution of the nuclear speckle protein SC35 to cytoplasmic foci...
  31. Jawhar M, Schwaab J, Hausmann D, Clemens J, Naumann N, Henzler T, et al. Splenomegaly, elevated alkaline phosphatase and mutations in the SRSF2/ASXL1/RUNX1 gene panel are strong adverse prognostic markers in patients with systemic mastocytosis. Leukemia. 2016;30:2342-2350 pubmed publisher
    ..We conclude that splenomegaly, elevated AP and mutations in the S/A/R gene panel are independent of the World Health Organization classification and provide the most relevant prognostic information in SM patients. ..
  32. Akbarzadeh A, Günther O, Houde A, Li S, Ming T, Jeffries K, et al. Developing specific molecular biomarkers for thermal stress in salmonids. BMC Genomics. 2018;19:749 pubmed publisher
    ..Eight genes, including two transcripts of each SERPINH1 and HSP90AA1, FKBP10, MAP3K14, SFRS2, and EEF2 showed strong and robust chronic temperature stress response consistently in the discovery analysis and ..
  33. Fei J, Jadaliha M, Harmon T, Li I, Hua B, Hao Q, et al. Quantitative analysis of multilayer organization of proteins and RNA in nuclear speckles at super resolution. J Cell Sci. 2017;130:4180-4192 pubmed publisher
    ..We found that SON and SC35 (also known as SRSF2) localize to the central region of the speckle, whereas MALAT1 and small nuclear (sn)..
  34. Zhang W, Wu J. Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing. Mol Cell Biol. 1998;18:676-84 pubmed
    ..We report here identification and functional characterization of a new splicing factor, Sip1 (SC35-interacting protein 1)...
  35. Loh T, Moon H, Cho S, Jung D, Hong S, Kim D, et al. SC35 promotes splicing of the C5-V6-C6 isoform of CD44 pre-mRNA. Oncol Rep. 2014;31:273-9 pubmed publisher
    ..However, the splicing regulation of the V6 exon is not completely understood. SC35 is an arginine-serine rich protein that regulates alternative splicing of various pre-mRNAs...
  36. Bermingham J, Arden K, Naumova A, Sapienza C, Viars C, Fu X, et al. Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2). Genomics. 1995;29:70-9 pubmed
    ..3-q22. Recombinant inbred mapping of the mouse ASF/SF2 gene (Sfrs1 locus) and the mouse SC-35 gene (Sfrs2 locus) demonstrates that both genes are located in a part of mouse chromosome 11 that is homologous to human ..
  37. Yan Q, Xia X, Sun Z, Fang Y. Depletion of Arabidopsis SC35 and SC35-like serine/arginine-rich proteins affects the transcription and splicing of a subset of genes. PLoS Genet. 2017;13:e1006663 pubmed publisher
    ..We found that loss of the functions of SC35 and SC35-like (SCL) proteins cause pleiotropic changes in plant morphology and development, including serrated ..
  38. Bøe S, Bjørndal B, Røsok B, Szilvay A, Kalland K. Subcellular localization of human immunodeficiency virus type 1 RNAs, Rev, and the splicing factor SC-35. Virology. 1998;244:473-82 pubmed
    ..The findings suggest that Rev and HIV-1 RNAs interact at putative sites of mRNA transcription and splicing. ..
  39. Lai M, Tarn W. Hypophosphorylated ASF/SF2 binds TAP and is present in messenger ribonucleoproteins. J Biol Chem. 2004;279:31745-9 pubmed
    ..Thus, the phosphorylation state of RS domains may modulate the function of mammalian shuttling SR proteins during mRNA maturation or export. ..
  40. Gelsi Boyer V, Cervera N, Bertucci F, Brecqueville M, Finetti P, Murati A, et al. Molecular similarity between myelodysplastic form of chronic myelomonocytic leukemia and refractory anemia with ring sideroblasts. Haematologica. 2013;98:576-83 pubmed publisher
  41. Tripathi V, Sixt K, Gao S, Xu X, Huang J, Weigert R, et al. Direct Regulation of Alternative Splicing by SMAD3 through PCBP1 Is Essential to the Tumor-Promoting Role of TGF-?. Mol Cell. 2016;64:549-564 pubmed publisher
    ..We show that TGF-? and EGF respectively induce SMAD3 and PCBP1 to colocalize in SC35-positive nuclear speckles, and the two proteins interact in the variable exon region of CD44 pre-mRNA to inhibit ..
  42. Ulfig N, Briese V. Immunolabelling of spliceosomes in sections and cultured astrocytes of human fetal brain tissue. Brain Dev. 1999;21:51-8 pubmed
    ..These spliceosomes consist among others of protein factors, such as the splicing factor SC35 being abundant in speckled regions of the cell nucleus...
  43. Tripathi K, Parnaik V. Differential dynamics of splicing factor SC35 during the cell cycle. J Biosci. 2008;33:345-54 pubmed
    ..We analysed the dynamics of the splicing factor SC35 in interphase and mitotic cells...
  44. Goldie B, Fitzsimmons C, Weidenhofer J, Atkins J, Wang D, Cairns M. miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif. Front Mol Neurosci. 2017;10:259 pubmed publisher
    ..and neural function, whereas Ago1-transcripts were associated with general metabolic processes and localized with SC35 spliceosomes...
  45. Mole S, McFarlane M, Chuen Im T, Milligan S, Millan D, Graham S. RNA splicing factors regulated by HPV16 during cervical tumour progression. J Pathol. 2009;219:383-91 pubmed publisher
    ..up-regulation of a specific subset of SR proteins and present evidence that HPV16 E2 controls expression of SRp20, SC35 and SRp75...
  46. Pawlicki J, Steitz J. Subnuclear compartmentalization of transiently expressed polyadenylated pri-microRNAs: processing at transcription sites or accumulation in SC35 foci. Cell Cycle. 2009;8:345-56 pubmed
    ..We also observed that transiently expressed pri-miRNAs accumulate in nuclear foci with splicing factor SC35 and Microprocessor components, Drosha and DGCR8...
  47. Qian W, Iqbal K, Grundke Iqbal I, Gong C, Liu F. Splicing factor SC35 promotes tau expression through stabilization of its mRNA. FEBS Lett. 2011;585:875-80 pubmed publisher
    ..We found that in addition to promoting tau exon 10 inclusion, splicing factor SC35 also promoted tau expression in HEK-293T cells...
  48. Chen C, Jin N, Qian W, Liu W, Tan X, Ding F, et al. Cyclic AMP-dependent protein kinase enhances SC35-promoted Tau exon 10 inclusion. Mol Neurobiol. 2014;49:615-24 pubmed publisher
    ..Disturbances in the ratio result in neurodegenerative tauopathies. Splicing factor SC35 acts on a SC35-like element located at the 5' end of tau exon 10 and promotes tau exon 10 inclusion...
  49. Lee Y, Chen H, Peres J, Gomez Deza J, Attig J, Stalekar M, et al. Hexanucleotide repeats in ALS/FTD form length-dependent RNA foci, sequester RNA binding proteins, and are neurotoxic. Cell Rep. 2013;5:1178-86 pubmed publisher
    ..The foci colocalize with a subset of RNA binding proteins, including SF2, SC35, and hnRNP-H in transfected cells...
  50. Park R, Miller G. Epstein-Barr Virus-Induced Nodules on Viral Replication Compartments Contain RNA Processing Proteins and a Viral Long Noncoding RNA. J Virol. 2018;92: pubmed publisher
    ..during lytic infection, before formation of viral replication compartments, two cellular pre-mRNA splicing factors, SC35 and SON, were dispersed from interchromatin granule clusters, and three mRNA export factors, Y14, ALY, and NXF1, ..
  51. Matsuyama H, Minami H, Sakaki T, Kasahara H, Watanabe A, Onoda T, et al. Psychrobacter oceani sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol. 2015;65:1450-5 pubmed publisher
    ..piscatorii T-3-2(T) (97.7%), P. nivimaris 88/2-7(T) (97.7%), P. celer SW-238(T) (97.7%), P. aestuarii SC35(T) (97.6%) and P. vallis CMS39(T) (97.6%). DNA-DNA hybridization between strain 4k5(T) and P...
  52. Pascarella A, Ferrandino G, Credendino S, Moccia C, D Angelo F, Miranda B, et al. DNAJC17 is localized in nuclear speckles and interacts with splicing machinery components. Sci Rep. 2018;8:7794 pubmed publisher
    ..Accordingly, co-localization of DNAJC17 with SC35, a marker of nuclear speckles, further supported its interaction with spliceosomal components...
  53. Wang H, Lin W, Dyck J, Yeakley J, Songyang Z, Cantley L, et al. SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. J Cell Biol. 1998;140:737-50 pubmed
    ..Together, our studies suggest that different SRPK family members may be uniquely regulated and targeted, thereby contributing to splicing regulation in different tissues, during development, or in response to signaling. ..
  54. Hall L, Smith K, Byron M, Lawrence J. Molecular anatomy of a speckle. Anat Rec A Discov Mol Cell Evol Biol. 2006;288:664-75 pubmed
    ..Nuclear speckles (aka SC35 domains) are essentially ubiquitous structures enriched for most pre-mRNA metabolic factors, yet their relationship ..
  55. Edmond V, Merdzhanova G, Gout S, Brambilla E, Gazzeri S, Eymin B. A new function of the splicing factor SRSF2 in the control of E2F1-mediated cell cycle progression in neuroendocrine lung tumors. Cell Cycle. 2013;12:1267-78 pubmed publisher
    ..Taken together, these results identify a new role of SRSF2 in the control of cell cycle progression and reinforce the functional link between SRSF2 and E2F1 proteins. ..
  56. Lin J, Yang J, Wen X, Yang L, Deng Z, Qian Z, et al. Detection of SRSF2-P95 mutation by high-resolution melting curve analysis and its effect on prognosis in myelodysplastic syndrome. PLoS ONE. 2014;9:e115693 pubmed publisher
    ..SRSF2 mutations were significantly associated with mortality rate in the MDS affected Chinese. ..
  57. Chen I, Sciabica K, Sandri Goldin R. ICP27 interacts with the RNA export factor Aly/REF to direct herpes simplex virus type 1 intronless mRNAs to the TAP export pathway. J Virol. 2002;76:12877-89 pubmed
    ..During infection, Aly/REF was no longer associated with splicing factor SC35 but moved into structures that colocalized with ICP27, suggesting that ICP27 recruits Aly/REF from ..
  58. Jacquenet S, Decimo D, Muriaux D, Darlix J. Dual effect of the SR proteins ASF/SF2, SC35 and 9G8 on HIV-1 RNA splicing and virion production. Retrovirology. 2005;2:33 pubmed
    ..production, we used a human cell line expressing high levels of complete HIV-1 and either one of the ASF/SF2, SC35, and 9G8 SR proteins...
  59. Lin C, Hou H, Chou W, Kuo Y, Liu C, Chen C, et al. IDH mutations are closely associated with mutations of DNMT3A, ASXL1 and SRSF2 in patients with myelodysplastic syndromes and are stable during disease evolution. Am J Hematol. 2014;89:137-44 pubmed publisher
    ..IDH mutations are stable during the clinical course. The mutation, in association with other genetic alterations, may play a role in the development, but not progression of MDS. ..
  60. Visconte V, Tabarroki A, Zhang L, Hasrouni E, Gerace C, Frum R, et al. Clinicopathologic and molecular characterization of myeloid neoplasms harboring isochromosome 17(q10). Am J Hematol. 2014;89:862 pubmed publisher
  61. JakubauskienÄ— E, Peciuliene I, Vilys L, Mocevicius P, Vilkaitis G, Kanopka A. Gastrointestinal tract tumors and cell lines possess differential splicing factor expression and tumor associated mRNA isoform formation profiles. Cancer Biomark. 2015;15:575-81 pubmed publisher
    ..Not all experimental results obtained in cell lines reflect changes that occur in real tumors. ..
  62. Pellagatti A, Boultwood J. Splicing factor gene mutations in the myelodysplastic syndromes: impact on disease phenotype and therapeutic applications. Adv Biol Regul. 2017;63:59-70 pubmed publisher
    ..Importantly, recent evidence suggests that spliceosome inhibitors and splicing modulators may have therapeutic value in the treatment of splicing factor mutant myeloid malignancies. ..
  63. Robertson A, Shih J, Yau C, Gibb E, Oba J, Mungall K, et al. Integrative Analysis Identifies Four Molecular and Clinical Subsets in Uveal Melanoma. Cancer Cell. 2017;32:204-220.e15 pubmed publisher
    ..Within D3-UM, EIF1AX- and SRSF2/SF3B1-mutant tumors have distinct somatic copy number alterations and DNA methylation profiles, providing insight into the biology of these low- versus intermediate-risk clinical mutation subtypes. ..
  64. Sokół E, Kedzierska H, Czubaty A, Rybicka B, Rodzik K, Tanski Z, et al. microRNA-mediated regulation of splicing factors SRSF1, SRSF2 and hnRNP A1 in context of their alternatively spliced 3'UTRs. Exp Cell Res. 2018;363:208-217 pubmed publisher
    ..The microRNAs targeting SRSF1 and SRSF2 are involved in a regulatory feedback loop. microRNAs miR-183-5p and miR-200c-3p that target SRSF2, affect the expression of genes involved in apoptotic regulation. ..
  65. Shakyawar D, Muralikrishna B, Radha V. C3G dynamically associates with nuclear speckles and regulates mRNA splicing. Mol Biol Cell. 2018;: pubmed publisher
    ..Here we show that C3G localizes to SC35 positive nuclear speckles, and regulates splicing activity...
  66. Imai Y, Matsuo N, Ogawa S, Tohyama M, Takagi T. Cloning of a gene, YT521, for a novel RNA splicing-related protein induced by hypoxia/reoxygenation. Brain Res Mol Brain Res. 1998;53:33-40 pubmed
    ..Far Western blot analysis showed that YT521 protein was not only interacting with RA301 protein, but also with SC35 and SF2, both of which are splicing factors...
  67. Pongoski J, Asai K, Cochrane A. Positive and negative modulation of human immunodeficiency virus type 1 Rev function by cis and trans regulators of viral RNA splicing. J Virol. 2002;76:5108-20 pubmed
  68. Expert Bezançon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer J, et al. hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 2004;279:38249-59 pubmed
    ..chromatography, we identified heterogeneous nuclear ribonucleoprotein (hnRNP) A1, the SR proteins ASF/SF2 and SC35, and hnRNP F/H as specific components that are assembled onto the G-rich element...
  69. Rice B, KADDIS R, Stake M, Lochmann T, Parent L. Interplay between the alpharetroviral Gag protein and SR proteins SF2 and SC35 in the nucleus. Front Microbiol. 2015;6:925 pubmed publisher
    ..L219A foci appeared to be tethered in the nucleus, partially co-localizing with the splicing speckle components SC35 and SF2. Overexpression of SC35 increased the number of Gag...
  70. Yamamoto K, Furukawa M, Fukumura K, Kawamura A, Yamada T, Suzuki H, et al. Control of the heat stress-induced alternative splicing of a subset of genes by hnRNP K. Genes Cells. 2016;21:1006-14 pubmed publisher
    ..Among the latter group, we found that SRp38/SRSF10 and SC35/SRSF2 are necessary for the inclusion of exon 13 of TNRC6A during heat stress...
  71. Bai J, Qu Y, Cao Y, Yang L, Ge L, Jin Y, et al. The SMN1 common variant c.22 dupA in Chinese patients causes spinal muscular atrophy by nonsense-mediated mRNA decay in humans. Gene. 2018;644:49-55 pubmed publisher
    ..b>SC35 1.7-kb transcripts, a physiological NMD substrate was determined to be a NMD positive gene in our experiments...
  72. Yin X, Jiang X, Wang J, Qian S, Liu F, Qian W. SIRT1 Deacetylates SC35 and Suppresses Its Function in Tau Exon 10 Inclusion. J Alzheimers Dis. 2017;: pubmed publisher
    ..b>Splicing factor SC35 (also name as SRSF2) plays an important role in promoting tau exon 10 inclusion...
  73. Vellard M, Sureau A, Soret J, Martinerie C, Perbal B. A potential splicing factor is encoded by the opposite strand of the trans-spliced c-myb exon. Proc Natl Acad Sci U S A. 1992;89:2511-5 pubmed
    ..We now report that a putative splicing factor (PR264), extremely well conserved in chicken and human, is encoded by the opposite strand of the c-myb trans-spliced exon...
  74. Sureau A, Soret J, Guyon C, Gaillard C, Dumon S, Keller M, et al. Characterization of multiple alternative RNAs resulting from antisense transcription of the PR264/SC35 splicing factor gene. Nucleic Acids Res. 1997;25:4513-22 pubmed
    The PR264/SC35 splicing factor belongs to the family of SR proteins which function as essential and alternative splicing factors. Here, we report that the human PR264/SC35 locus is bidirectionally transcribed...
  75. Kavanaugh G, Wise Draper T, Morreale R, Morrison M, Gole B, Schwemberger S, et al. The human DEK oncogene regulates DNA damage response signaling and repair. Nucleic Acids Res. 2011;39:7465-76 pubmed publisher
    ..Taken together, the data demonstrate new molecular links between DEK and DNA damage response signaling pathways, and suggest that DEK contributes to DNA repair. ..
  76. McCuaig R, Dunn J, Li J, Masch A, Knaute T, Schutkowski M, et al. PKC-Theta is a Novel SC35 Splicing Factor Regulator in Response to T Cell Activation. Front Immunol. 2015;6:562 pubmed publisher
    ..Collectively, our findings suggest that nuclear PKC-θ is a novel regulator of the key splicing factor SC35 in T cells.
  77. Skrdlant L, Stark J, Lin R. Myelodysplasia-associated mutations in serine/arginine-rich splicing factor SRSF2 lead to alternative splicing of CDC25C. BMC Mol Biol. 2016;17:18 pubmed publisher
    ..SRSF2 P95 mutants lead to alternative splicing of CDC25C in a manner that is not dependent on the DNA damage response. ..
  78. Hsu P, Hsi E, Wang T, Lin R, Liao Y, Juo S. MicroRNA let-7g possesses a therapeutic potential for peripheral artery disease. J Cell Mol Med. 2017;21:519-529 pubmed publisher
    ..In addition, let-7g affected the splicing factor SC35 which subsequently enhanced the alternative splicing of VEGF-A from the anti-angiogenic isoform VEGF-A..
  79. Bandara Y, Weerasooriya D, Liu S, Little C. The necrotrophic fungus Macrophomina phaseolina promotes charcoal rot susceptibility in grain sorghum through induced host cell wall-degrading enzymes. Phytopathology. 2018;: pubmed publisher
    ..validation, crude enzymes mixtures (CEM) were extracted from MP- and mock-inoculated charcoal rot resistant (SC599, SC35) and susceptible (Tx7000, BTx3042) sorghum genotype stalks...
  80. Parfenov V, Davis D, Pochukalina G, Kostyuchek D, Murti K. Dynamics of distribution of splicing components relative to the transcriptional state of human oocytes from antral follicles. J Cell Biochem. 1998;69:72-80 pubmed
    ..These data are consistent with the notion that splicing occurs in the karyoplasm and that the splicing components are mobilized from a storage site (IGCs) to the site of action. ..
  81. van den IJssel P, Wheelock R, Prescott A, Russell P, Quinlan R. Nuclear speckle localisation of the small heat shock protein alpha B-crystallin and its inhibition by the R120G cardiomyopathy-linked mutation. Exp Cell Res. 2003;287:249-61 pubmed
    ..While alpha B-crystallin was preferentially localised to speckles as shown by colocalisation with non-snRNP, SC35, as well as the snRNP components Sm and U1A, HSP27 was also seen associated with the nucleolar compartment, ..
  82. Yang L, Li N, Wang C, Yu Y, Yuan L, Zhang M, et al. Cyclin L2, a novel RNA polymerase II-associated cyclin, is involved in pre-mRNA splicing and induces apoptosis of human hepatocellular carcinoma cells. J Biol Chem. 2004;279:11639-48 pubmed
    ..The data suggest that cyclin L2 represents a new member of the cyclin family, which might regulate the transcription and RNA processing of certain apoptosis-related factors, resulting in tumor cell growth inhibition and apoptosis. ..
  83. Fan C, Chen Q, Wang Q. Functional role of transcriptional factor TBX5 in pre-mRNA splicing and Holt-Oram syndrome via association with SC35. J Biol Chem. 2009;284:25653-63 pubmed publisher
    ..Proteomics in combination with affinity purification identifies splicing factor SC35 as a candidate TBX5-associating protein...
  84. Shepard P, Hertel K. The SR protein family. Genome Biol. 2009;10:242 pubmed publisher
    ..These activities point up the importance of SR proteins during the regulation of mRNA metabolism. ..
  85. Yi L, Gao B, Gao F, Fu F, Zhang X, Sun J. [Splicing factor SC35 regulated the expression of B7-H3 in vitro]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012;28:815-7 pubmed
    To explore the mechanisms by which splicing factor SC35 regulates the costimulatory molecule B7-H3 expression in vitro through bioinformatic and molecular biological methods...
  86. Melko M, Nguyen L, Shaw M, Jolly L, Bardoni B, Gecz J. Loss of FMR2 further emphasizes the link between deregulation of immediate early response genes FOS and JUN and intellectual disability. Hum Mol Genet. 2013;22:2984-91 pubmed publisher
    ..These results suggest that FMR2 is an upstream regulator of FOS and JUN, and further link deregulation of the immediate early response genes to the pathology of ID- and FRAXE-associated ID in particular. ..
  87. Lehmann U, Bartels S, Hasemeier B, Geffers R, Schlue J, Büsche G, et al. SRSF2 mutation is present in the hypercellular and prefibrotic stage of primary myelofibrosis. Blood. 2013;121:4011-2 pubmed publisher
  88. Garza E, Fabiani E, Noguera N, Panetta P, Piredda M, Borgia L, et al. Development of a high-resolution melting curve analysis screening test for SRSF2 splicing factor gene mutations in myelodysplastic syndromes. J Mol Diagn. 2015;17:85-9 pubmed publisher
    ..This rapid screening method may provide useful information for clinical decision making and be helpful to optimize laboratory resources and reduce turnaround time. ..
  89. Workenhe S, Ketela T, Moffat J, Cuddington B, Mossman K. Genome-wide lentiviral shRNA screen identifies serine/arginine-rich splicing factor 2 as a determinant of oncolytic virus activity in breast cancer cells. Oncogene. 2016;35:2465-74 pubmed publisher
  90. Zhang J, Lieu Y, Ali A, Penson A, Reggio K, Rabadan R, et al. Disease-associated mutation in SRSF2 misregulates splicing by altering RNA-binding affinities. Proc Natl Acad Sci U S A. 2015;112:E4726-34 pubmed publisher
    ..Our findings shed light on the mechanism of the disease-associated SRSF2 mutation in splicing regulation and also reveal a group of misspliced mRNA isoforms for potential therapeutic targeting. ..
  91. Carlson S, Soulette C, Yang Z, Elias J, Brooks A, Gozani O. RBM25 is a global splicing factor promoting inclusion of alternatively spliced exons and is itself regulated by lysine mono-methylation. J Biol Chem. 2017;292:13381-13390 pubmed publisher
    ..Together, our findings uncover a pivotal role for RBM25 as an essential regulator of alternative splicing and reveal a new potential mechanism for regulation of pre-mRNA splicing by lysine methylation of a splicing factor. ..