SBDS

Summary

Gene Symbol: SBDS
Description: SBDS, ribosome maturation factor
Alias: CGI-97, SDS, SWDS, ribosome maturation protein SBDS, SBDS, ribosome assembly guanine nucleotide exchange factor
Species: human
Products:     SBDS

Top Publications

  1. Woloszynek J, Rothbaum R, Rawls A, Minx P, Wilson R, Mason P, et al. Mutations of the SBDS gene are present in most patients with Shwachman-Diamond syndrome. Blood. 2004;104:3588-90 pubmed
    ..Recent studies show that mutations of SBDS, a gene of unknown function, are present in the majority of patients with SDS...
  2. Ganapathi K, Austin K, Lee C, Dias A, Malsch M, Reed R, et al. The human Shwachman-Diamond syndrome protein, SBDS, associates with ribosomal RNA. Blood. 2007;110:1458-65 pubmed
    ..Mutations in the SBDS gene are identified in most patients with SDS. SBDS encodes a highly conserved protein of unknown function...
  3. Austin K, Gupta M, Coats S, Tulpule A, Mostoslavsky G, Balazs A, et al. Mitotic spindle destabilization and genomic instability in Shwachman-Diamond syndrome. J Clin Invest. 2008;118:1511-8 pubmed publisher
    Deficiencies in the SBDS gene result in Shwachman-Diamond syndrome (SDS), an inherited bone marrow failure syndrome associated with leukemia predisposition...
  4. Ball H, Zhang B, Riches J, Gandhi R, Li J, Rommens J, et al. Shwachman-Bodian Diamond syndrome is a multi-functional protein implicated in cellular stress responses. Hum Mol Genet. 2009;18:3684-95 pubmed publisher
    ..syndrome (SDS; OMIM 260400) results from loss-of-function mutations in the Shwachman-Bodian Diamond syndrome (SBDS) gene...
  5. Orelio C, Verkuijlen P, Geissler J, van den Berg T, Kuijpers T. SBDS expression and localization at the mitotic spindle in human myeloid progenitors. PLoS ONE. 2009;4:e7084 pubmed publisher
    Shwachman-Diamond Syndrome (SDS) is a hereditary disease caused by mutations in the SBDS gene. SDS is clinically characterized by pancreatic insufficiency, skeletal abnormalities and bone marrow dysfunction...
  6. Kuijpers T, Alders M, Tool A, Mellink C, Roos D, Hennekam R. Hematologic abnormalities in Shwachman Diamond syndrome: lack of genotype-phenotype relationship. Blood. 2005;106:356-61 pubmed
    ..The causative SBDS gene was sequenced in 20 of 23 unrelated patients with clinical SDS...
  7. Kawakami T, Mitsui T, Kanai M, Shirahata E, Sendo D, Kanno M, et al. Genetic analysis of Shwachman-Diamond syndrome: phenotypic heterogeneity in patients carrying identical SBDS mutations. Tohoku J Exp Med. 2005;206:253-9 pubmed
    ..Recently, the cause of SDS was identified as mutations of Shwachman-Bodian-Diamond syndrome gene (SBDS) and most mutations are caused by gene conversion between SBDS and its highly homologous pseudogene...
  8. Hesling C, Oliveira C, Castilho B, Zanchin N. The Shwachman-Bodian-Diamond syndrome associated protein interacts with HsNip7 and its down-regulation affects gene expression at the transcriptional and translational levels. Exp Cell Res. 2007;313:4180-95 pubmed
    ..SDS has been associated to mutations in the SBDS gene, encoding a highly conserved protein that was shown to function in ribosome biogenesis in yeast...
  9. Boocock G, Morrison J, Popovic M, Richards N, Ellis L, Durie P, et al. Mutations in SBDS are associated with Shwachman-Diamond syndrome. Nat Genet. 2003;33:97-101 pubmed publisher
    ..Here, we report identification of disease-associated mutations in an uncharacterized gene, SBDS, in the interval of 1.9 cM at 7q11 previously shown to be associated with the disease. We report that SBDS has a 1...

More Information

Publications134 found, 100 shown here

  1. Stepanovic V, Wessels D, Goldman F, Geiger J, Soll D. The chemotaxis defect of Shwachman-Diamond Syndrome leukocytes. Cell Motil Cytoskeleton. 2004;57:158-74 pubmed
    ..While mutations at a single gene locus (SBDS) appear to be responsible for SDS in a majority of patients, the function of that gene and a specific defect in SDS ..
  2. Austin K, Leary R, Shimamura A. The Shwachman-Diamond SBDS protein localizes to the nucleolus. Blood. 2005;106:1253-8 pubmed
    ..The gene for this syndrome, SBDS, encodes a highly conserved novel protein...
  3. de Oliveira J, Sforça M, Blumenschein T, Goldfeder M, Guimarães B, Oliveira C, et al. Structure, dynamics, and RNA interaction analysis of the human SBDS protein. J Mol Biol. 2010;396:1053-69 pubmed publisher
    ..This syndrome has been associated with mutations in the SBDS gene, which encodes a conserved protein showing orthologs in Archaea and eukaryotes...
  4. Loshaj Shala A, Colzani M, Brezovska K, Poceva Panovska A, Suturkova L, Beretta G. Immunoproteomic identification of antigenic candidate Campylobacter jejuni and human peripheral nerve proteins involved in Guillain-Barré syndrome. J Neuroimmunol. 2018;317:77-83 pubmed publisher
    ..Proteins isolated from C. jejuni and human peripheral nerve tissue (HPN) were separated with 2D SDS-PAGE and subjected to western blotting using serum samples from GBS patients...
  5. Seo Y, Jung Y, Kim S. Decellularized heart ECM hydrogel using supercritical carbon dioxide for improved angiogenesis. Acta Biomater. 2018;67:270-281 pubmed publisher
    ..Decellularization by various detergents such as sodium dodecyl sulfate (SDS) and triton X-100 can remove the cell nuclei in tissue organs...
  6. Huang H, Zeng Z, Wang K, Zhang H, Wang S, Zhou W, et al. Heme oxygenase-1 protects rat liver against warm ischemia/reperfusion injury via TLR2/TLR4-triggered signaling pathways. World J Gastroenterol. 2015;21:2937-48 pubmed publisher
    ..TRIF) and anti-myeloid differentiation factor 88 (MyD88), and then the immunoprecipitates were analyzed by SDS-PAGE and immunoblotted with the indicated antibodies...
  7. Wang Y, Li Y, Zhang B, Zhang F. The preclinical evaluation of immunocontraceptive vaccines based on canine zona pellucida 3 (cZP3) in a mouse model. Reprod Biol Endocrinol. 2018;16:47 pubmed publisher
    ..The fusion proteins OGZ and OZ were identified by SDS-PAGE and Western blot. Mice were immunized with OGZ, OZ and cZP3, and the infertility rates were monitored...
  8. Ng C, Waterman D, Koonin E, Walters A, Chong J, Isupov M, et al. Conformational flexibility and molecular interactions of an archaeal homologue of the Shwachman-Bodian-Diamond syndrome protein. BMC Struct Biol. 2009;9:32 pubmed publisher
    Defects in the human Shwachman-Bodian-Diamond syndrome (SBDS) protein-coding gene lead to the autosomal recessive disorder characterised by bone marrow dysfunction, exocrine pancreatic insufficiency and skeletal abnormalities...
  9. Stepensky P, Chacón Flores M, Kim K, Abuzaitoun O, Bautista Santos A, Simanovsky N, et al. Mutations in EFL1, an SBDS partner, are associated with infantile pancytopenia, exocrine pancreatic insufficiency and skeletal anomalies in aShwachman-Diamond like syndrome. J Med Genet. 2017;54:558-566 pubmed publisher
    ..Its release in the cytoplasm requires the interaction of EFL1 and SDBS proteins. In Shwachman-Diamond syndrome (SDS), a defective SDBS protein prevents eIF6 eviction, inhibiting its recycle to the nucleus and subsequent formation ..
  10. Bezerra M, Arruda Alencar J, Martins J, Viana A, Viana Neto A, Rego J, et al. Major seminal plasma proteome of rabbits and associations with sperm quality. Theriogenology. 2019;128:156-166 pubmed publisher
    ..Semen samples were collected from 18 New Zealand adult rabbits, and seminal plasma proteins were analyzed by 2-D SDS-PAGE and tandem mass spectrometry...
  11. Hu X, Wei B, Song Y, Ji Q, Li Q, Luo Y, et al. Plasma metabolic profiling on postoperative colorectal cancer patients with different traditional Chinese medicine syndromes. Complement Ther Med. 2018;36:14-19 pubmed publisher
    ..controls (HC) and 70 PCRC patients, including 10 Dampness and heat syndrome (DHS), 33 Spleen deficiency syndrome (SDS), 19 Liver and kidney Yin deficiency syndrome (LKYDS) and 8 with non-TCM syndrome (NS) were enrolled...
  12. Pereira B, Brazón J. Aqueous extract from Brownea grandiceps flowers with effect on coagulation and fibrinolytic system. J Ethnopharmacol. 2015;160:6-13 pubmed publisher
    ..In addition to that, the fibrinogenolytic and fibronectinase activity was evaluated by SDS-PAGE using Tris-Tricine system and analyzed by densitometric study utilizing ImageJ program...
  13. Choi J, Jang Y, Oh J, Kim C, Hyun I. Bee Pollen-Induced Anaphylaxis: A Case Report and Literature Review. Allergy Asthma Immunol Res. 2015;7:513-7 pubmed publisher
    ..Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and IgE-immunoblot analysis revealed 9 protein bands (11, 14, 17, 28, 34, 45, 52, 72, and 90 kDa) and strong ..
  14. Islam K, Bond J, Fakhoury A. FvSNF1, the sucrose non-fermenting protein kinase gene of Fusarium virguliforme, is required for cell-wall-degrading enzymes expression and sudden death syndrome development in soybean. Curr Genet. 2017;63:723-738 pubmed publisher
    Fusarium virguliforme is a soil-borne pathogenic fungus that causes sudden death syndrome (SDS) in soybean. Its pathogenicity is believed to require the activity of cell-wall-degrading enzymes (CWDEs)...
  15. Pepper S, Britz M. An Acid Up-Regulated Surface Protein of Lactobacillus paracasei Strain GCRL 46 is Phylogenetically Related to the Secreted Glucan- (GpbB) and Immunoglobulin-Binding (SibA) Protein of Pathogenic Streptococci. Int J Mol Sci. 2019;20: pubmed publisher
    ..0 versus optimum growth pH of 6.3. Up-regulation of proteins in this size range was not detected in SDS-PAGE gels of the cytosolic fraction, but was routinely detected following growth at low pH in whole cells and cell ..
  16. Kong X, Li W, Guo L, Zhang J, Chen X, Liu W, et al. Sesamin enhances nitric oxide bioactivity in aortas of spontaneously hypertensive rats. Ther Adv Cardiovasc Dis. 2015;9:314-24 pubmed publisher
    ..form of eNOS in aortas was determined by low-temperature sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)...
  17. André V, Longoni D, Bresolin S, Cappuzzello C, Dander E, Galbiati M, et al. Mesenchymal stem cells from Shwachman-Diamond syndrome patients display normal functions and do not contribute to hematological defects. Blood Cancer J. 2012;2:e94 pubmed publisher
    Shwachman-Diamond syndrome (SDS) is a rare inherited disorder characterized by bone marrow (BM) dysfunction and exocrine pancreatic insufficiency...
  18. Yetim A, Yetim Ç, Bas F, Erol O, Çığ G, Ucar A, et al. Anti-Müllerian Hormone and Inhibin-A, but not Inhibin-B or Insulin-Like Peptide-3, may be Used as Surrogates in the Diagnosis of Polycystic Ovary Syndrome in Adolescents: Preliminary Results. J Clin Res Pediatr Endocrinol. 2016;8:288-97 pubmed publisher
    ..001, p<0.001, respectively). In multiple linear regression analysis, WC SDS (p=0.028), logD4-A (p=0.033), logSHBG (p=0.031), and total ovarian volume (p=0...
  19. Zhang Y, Guo K, Ji S, Liu X, Wang P, Wu J, et al. Development and characterization of a novel long-acting recombinant follicle stimulating hormone agonist by fusing Fc to an FSH-β subunit. Hum Reprod. 2016;31:169-82 pubmed publisher
    ..The purity and molecular weight of KN015 were determined by reducing and non-reducing SDS-PAGE...
  20. Yang E, Yi S, Bai F, Niu D, Zhong J, Wu Q, et al. Cloning, Characterization and Expression Pattern Analysis of a Cytosolic Copper/Zinc Superoxide Dismutase (SaCSD1) in a Highly Salt Tolerant Mangrove (Sonneratia alba). Int J Mol Sci. 2015;17: pubmed publisher
    ..glycerol, and chloroform, and was reduced to a great extent in β-mercaptoethanol, sodium dodecyl sulfate (SDS), H₂O2, and phenol. Moreover, the SaCSD1 protein was very susceptive to pepsin digestion...
  21. Snelling T, Wallace R. The rumen microbial metaproteome as revealed by SDS-PAGE. BMC Microbiol. 2017;17:9 pubmed publisher
    ..The aim of this study was to explore the metaproteome of bovine and ovine ruminal digesta using 2D SDS-PAGE...
  22. Garanty Bogacka B, Syrenicz M, Rac M, Krupa B, Czaja Bulsa G, Walczak M, et al. Association between serum osteocalcin, adiposity and metabolic risk in obese children and adolescents. Endokrynol Pol. 2013;64:346-52 pubmed publisher
    ..Age, gender, pubertal stage, adiposity markers (standard deviation score of body mass index: BMI-SDS, percentage of body fat, waist circumference), blood pressure, serum osteocalcin (OC), fasting plasma glucose and ..
  23. Ahmad A, Hamid R, Dada A, Usup G. Pseudomonas putida Strain FStm2 Isolated from Shark Skin: A Potential Source of Bacteriocin. Probiotics Antimicrob Proteins. 2013;5:165-75 pubmed publisher
    ..Instead, a positive effect on the relative activity of produced bacteriocin was observed as sodium dodecyl sulfate (SDS), EDTA, and Tween 20 at 1 % concentration all improved bacteriocin activity when the cell-free supernatant was ..
  24. Helling A, König H, Seiler F, Berkholz R, Thom V, Polakovic M. Retention of Acholeplasma laidlawii by Sterile Filtration Membranes: Effect of Cultivation Medium and Filtration Temperature. PDA J Pharm Sci Technol. 2018;72:264-277 pubmed publisher
    ..PFU/mL: Plaque-forming units per milliliter; PSD: Particle size distribution; PVP: Polyvinylpyrrolidone; SDS: Sodium dodecyl sulfate; SEM: Scanning electron microscopy; SLB: Saline lactose broth; TMP: Transmembrane pressure; ..
  25. Wojcieszek J, Ruzik L. Operationally defined species characterization and bioaccessibility evaluation of cobalt, copper and selenium in Cape gooseberry (Physalis Peruviana L.) by SEC-ICP MS. J Trace Elem Med Biol. 2016;34:15-21 pubmed publisher
    ..4), sodium dodecyl sulfate (SDS) (pH 7.4) and ammonium acetate (pH 5.5)...
  26. Gao C, Wang Y, Zhang Y, Lv M, Dou P, Xu P, et al. NAD-Independent L-Lactate Dehydrogenase Required for L-Lactate Utilization in Pseudomonas stutzeri A1501. J Bacteriol. 2015;197:2239-2247 pubmed publisher
    ..b>SDS-PAGE and successive matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) ..
  27. de Moura V, da Costa Guimarães N, Batista L, Freitas de Sousa L, de Sousa Martins J, de Souza M, et al. Assessment of the anti-snakebite properties of extracts of Aniba fragrans Ducke (Lauraceae) used in folk medicine as complementary treatment in cases of envenomation by Bothrops atrox. J Ethnopharmacol. 2018;213:350-358 pubmed publisher
    ..Interaction between the extracts and the proteins in BaV was assessed by electrophoresis (SDS-PAGE) and Western blot (WB). The cytotoxicity of the extracts was assessed in a strain of MRC-5 human fibroblasts...
  28. Yang X, Yan W, Tian Y, Ma P, Opperman L, Wang X. Family with sequence similarity member 20C is the primary but not the only kinase for the small-integrin-binding ligand N-linked glycoproteins in bone. FASEB J. 2016;30:121-8 pubmed publisher
    ..bone sialoprotein-, and dentin matrix protein-1-enriched fractions by anion-exchange chromatography and analyzed by SDS-PAGE, native PAGE, and Western immunoblot analysis...
  29. Sun Q, Zhang B, Yan Q, Jiang Z. Comparative analysis on the distribution of protease activities among fruits and vegetable resources. Food Chem. 2016;213:708-713 pubmed publisher
    ..8U/g), broccoli (16.9U/g), ginger (16.6U/g), leek (32.7U/g) and red pepper (15.8U/g) at different pH values. SDS-PAGE and zymograms confirmed that various types of proteases existed in the five plant extracts and might be ..
  30. Qiu W, Steinberg S. Phos-tag SDS-PAGE resolves agonist- and isoform-specific activation patterns for PKD2 and PKD3 in cardiomyocytes and cardiac fibroblasts. J Mol Cell Cardiol. 2016;99:14-22 pubmed publisher
    ..This study uses Phos-tag SDS-PAGE, a method that exaggerates phosphorylation-dependent mobility shifts, to overcome this technical limitation...
  31. Kahe H, Chamsaz M. A new supramolecular based liquid solid microextraction method for preconcentration and determination of trace bismuth in human blood serum and hair samples by electrothermal atomic absorption spectrometry. Environ Monit Assess. 2016;188:601 pubmed
    ..Catanionic microstructures of cetyltrimethylammonium bromide (CTAB) and sodium dodecyl sulfate (SDS) surfactants, dissolved in deionized water/propanol, are used as a green solvent to extract bismuth (III)-..
  32. Hemici A, Benerbaiha R, Bendjeddou D. Purification and biochemical characterization of a 22-kDa stable cysteine- like protease from the excretory-secretory product of the liver fluke Fasciola hepatica by using conventional techniques. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1068-1069:268-276 pubmed publisher
    ..1 and the overall activity yield reached a rate of 18.8%. The MW of the purified protease was estimated by reducing SDS-PAGE to be 22kDa while the proteolytic activity detection was carried out by zymography on non-denaturing SDS-PAGE ..
  33. Singhal S, Singhal J, Cheng J, Pikuła S, Sharma R, Zimniak P, et al. Purification and functional reconstitution of intact ral-binding Gtpase activating protein, RLIP76, in artificial liposomes. Acta Biochim Pol. 2001;48:551-62 pubmed
    ..The purity and identity of recombinant and human erythrocyte RLIP76 was established by SDS/PAGE and Western-blot analysis...
  34. Kumar S, Ali W, Bhattacharya S, Singh U, Kumar A, Verma A. The effect of elapsed time on cardiac troponin-T (cTnT) degradation and its relation to postmortem interval in cases of electrocution. J Forensic Leg Med. 2015;34:45-9 pubmed publisher
    ..at room temperature for different time periods (∼5, 26, 50, 84, 132, 157, 180, 205 and 230 Hrs), separation by SDS-PAGE and visualization by Western blot using cTnT specific monoclonal antibodies...
  35. Atkinson R, Fernandez Martos C, Atkin J, Vickers J, King A. Erratum: C9ORF72 expression and cellular localization over mouse development. Acta Neuropathol Commun. 2016;4:2 pubmed publisher
    ..the error is as follows: "Denatured protein samples (15 μg) from each time-point were electrophoresed into 10 % SDS-PAGE gels (BioRad), transferred to PVDF membranes (BioRad) and incubated in primary antibodies overnight (Table 1)"...
  36. Dörr H, Boguszewski M, Dahlgren J, Dunger D, Geffner M, Hokken Koelega A, et al. Short Children with CHARGE Syndrome: Do They Benefit from Growth Hormone Therapy?. Horm Res Paediatr. 2015;84:49-53 pubmed publisher
    ..3 μg/l and IGF-I level -2.01 standard deviation score (SDS). Sixteen subjects (9 boys) were followed longitudinally for 2 years. Birth length (median SDS, -0...
  37. Sarsaifi K, Haron A, Vejayan J, Yusoff R, Hani H, Omar M, et al. Two-dimensional polyacrylamide gel electrophoresis of Bali bull (Bos javanicus) seminal plasma proteins and their relationship with semen quality. Theriogenology. 2015;84:956-68 pubmed publisher
    ..Seminal plasma of both high-quality and low-quality Bali bulls was subjected to two-dimensional SDS-PAGE with isoelectric point ranged from 3 to 10 and molecular weight from 10 to 250 kDa...
  38. Huang D, Yao Y, Zhang H, Mei Z, Wang R, Feng L, et al. Directed optimization of a newly identified squalene synthase from Mortierella alpine based on sequence truncation and site-directed mutagenesis. J Ind Microbiol Biotechnol. 2015;42:1341-52 pubmed publisher
    ..Subsequently, the recombinant protein was expressed in Escherichia coli BL21(DE3) and detected by SDS-PAGE. To improve the expression and solubility of protein, 17 or 27 amino acids in the C-terminal were deleted...
  39. Bakhshi F, Pilehchian Langroudi R, Imani B. Enhanced expression of recombinant beta toxin of <i>Clostridium perfringens</i> type B using a commercially available <i>Escherichia coli</i> strain. Onderstepoort J Vet Res. 2016;83:e1-4 pubmed publisher
    ..5 mM, 1 mM, 1.5 mM IPTG. Expression was evaluated using sodium dodecyl sulfate Polyacrylamide gel electrophoresis (SDS-PAGE). The recombinant protein was purified via Ni-NTA and was analysed using western blot. We concluded that E...
  40. Lee K, Shim J, Yao Z, Kim J, Kim H, Kim J. Characterization of a Glutamate Decarboxylase (GAD) from Enterococcus avium M5 Isolated from Jeotgal, a Korean Fermented Seafood. J Microbiol Biotechnol. 2017;27:1216-1222 pubmed publisher
    ..Recombinant GAD was purified through a Ni-NTA column and the size was estimated to be 53 kDa by SDS-PAGE. Maximum GAD activity was observed at pH 4...
  41. Tanaka T, Nogariya O, Shionoiri N, Maeda Y, Arakaki A. Integrated molecular analysis of the inactivation of a non-enveloped virus, feline calicivirus, by UV-C radiation. J Biosci Bioeng. 2018;126:63-68 pubmed publisher
    ..Integrated molecular analyses using RT-qPCR, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and mass spectrometry were employed to evaluate the extent of ssRNA genome and protein degradation...
  42. Torbey E, Pachana N, Dissanayaka N. Depression rating scales in Parkinson's disease: A critical review updating recent literature. J Affect Disord. 2015;184:216-24 pubmed publisher
    ..The PHQ-2, PHQ-10, SDS, CES-D, UPDRS-Depression item, IDS-SR, and IDS-C each showed some evidence of validity or reliability, however ..
  43. Kajikawa A, Midorikawa E, Masuda K, Kondo K, Irisawa T, Igimi S, et al. Characterization of flagellins isolated from a highly motile strain of Lactobacillus agilis. BMC Microbiol. 2016;16:49 pubmed publisher
    ..Glycosylation of the flagellar filaments and their resistance to heat, acid and SDS were also observed...
  44. Malairaj S, Muthu S, Gopal V, Perumal P, Ramasamy R. Qualitative and quantitative determination of R-phycoerythrin from Halymenia floresia (Clemente) C. Agardh by polyacrylamide gel using electrophoretic elution technique. J Chromatogr A. 2016;1454:120-6 pubmed publisher
    ..The purified R-Phycoerythrin showed a single band on the examination by native PAGE electrophrosis. SDS-PAGE analysis showed five bands at 16kDa, 21kDa, 30kDa, 39kDa and 47kDa which corresponds to the ?, ? and ?', ? and ..
  45. Karamać M, Kosińska Cagnazzo A, Kulczyk A. Use of Different Proteases to Obtain Flaxseed Protein Hydrolysates with Antioxidant Activity. Int J Mol Sci. 2016;17: pubmed publisher
    ..hydrolysis products was profiled using Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Tricine-SDS-PAGE) and size exclusion-high performance liquid chromatography (SE-HPLC) separations...
  46. Oyama E, Takahashi H. Purification and characterization of two high molecular mass snake venom metalloproteinases (P-III SVMPs), named SV-PAD-2 and HR-Ele-1, from the venom of Protobothrops elegans (Sakishima-habu). Toxicon. 2015;103:30-8 pubmed publisher
    ..HR-Ele-1 appeared as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) regard under reducing and non-reducing conditions, and the molecular mass of this protease was approximately ..
  47. Tong Y, Feng S, Xin Y, Yang H, Zhang L, Wang W, et al. Enhancement of soluble expression of codon-optimized Thermomicrobium roseum sarcosine oxidase in Escherichia coli via chaperone co-expression. J Biotechnol. 2016;218:75-84 pubmed publisher
    ..coli. The TrSOX gene was successfully constructed in the pET28a plasmid. After induction with IPTG for 8h, SDS-PAGE analysis of crude enzyme solutions showed a significant 43 kDa protein band, indicating SOX was successfully ..
  48. Jee Y, Sowada N, Markello T, Rezvani I, Borck G, Baron J. BRF1 mutations in a family with growth failure, markedly delayed bone age, and central nervous system anomalies. Clin Genet. 2017;91:739-747 pubmed publisher
    ..A 10-year old boy was evaluated for impaired postnatal linear growth (height 113.3 cm, -4.6 SDS), a bone age that was delayed by 5 years, dysmorphic facies, cognitive impairment, and central nervous system ..
  49. Santos Filho N, Sousa T, Boldrini França J, Santos Silva L, Menaldo D, Henrique Silva F, et al. rBaltMIP, a recombinant alpha-type myotoxin inhibitor from Bothrops alternatus (Rhinocerophis alternatus) snake, as a potential candidate to complement the antivenom therapy. Toxicon. 2016;124:53-62 pubmed publisher
    ..Biochemical experiments showed that rBaltMIP presented pI 5.8 and molecular masses of ?21 kDa by SDS-PAGE and 19.57 kDa by MALDI/TOF MS...
  50. Lyke D, Manogaran A. Spatial sequestration and oligomer remodeling during de novo [PSI+] formation. Prion. 2017;11:332-337 pubmed publisher
    ..We also observed SDS-resistant oligomers during formation, and lysates containing newly formed oligomers can convert [psi-] ..
  51. Wuethrich A, Quirino J. Unusual stacking with electrokinetic injection of cationic analytes from micellar solutions in capillary zone electrophoresis. Anal Bioanal Chem. 2016;408:8663-8668 pubmed
    ..In this work, we describe the unusual stacking of cationic analytes via EKI of sodium dodecyl sulfate (SDS) micelles into a fused silica capillary filled with acidic background solution (BGS) with 40-50 % acetonitrile...
  52. South K, Freitas M, Lane D. Conformational quiescence of ADAMTS-13 prevents proteolytic promiscuity. J Thromb Haemost. 2016;14:2011-2022 pubmed publisher
    ..Methods Fibrinogen proteolysis was characterized using SDS PAGE and liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
  53. Ogada P, Kiirika L, Lorenz C, Senkler J, Braun H, Poehling H. Differential proteomics analysis of Frankliniella occidentalis immune response after infection with Tomato spotted wilt virus (Tospovirus). Dev Comp Immunol. 2017;67:1-7 pubmed publisher
    ..Two-dimensional isoelectric focusing/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D-IEF/SDS/PAGE) combined with mass spectrometry (MS), were used to identify proteins that were differentially expressed upon ..
  54. Hardy L, Mitchell C, Seabrooke T, Hogarth L. Drug cue reactivity involves hierarchical instrumental learning: evidence from a biconditional Pavlovian to instrumental transfer task. Psychopharmacology (Berl). 2017;234:1977-1984 pubmed publisher
    ..outcomes, respectively, and these response-outcome contingencies were reversed across two discriminative stimuli (SDs)...
  55. Panpetch P, Field R, Limpaseni T. Heterologous co-expression in E. coli of isoamylase genes from cassava Manihot esculenta Crantz 'KU50' achieves enzyme-active heteromeric complex formation. Plant Mol Biol. 2018;96:417-427 pubmed publisher
    ..rMeISA1 and rMeISA2 showed approximate molecular weights of 87 and 99 kDa, respectively, by SDS-PAGE...
  56. Wang J, Gao M, Yan G, Zhang X. An effective and in-situ method based tresyl-functionalized porous polymer material for enrichment and digestion of membrane proteins and its application in extraction tips. Anal Chim Acta. 2015;880:77-83 pubmed publisher
    ..protein groups) and 359 transmembrane proteins were identified by nano-LC-ESI-MS/MS in 4% sodium dodecyl sulfate (SDS), and similar results were achieved in the 60% methanol solution...
  57. Li C, Zhao B, Jiang Y, Diao J, Li N, Lu W. CONSTRUCTION AND EXPRESSION OF DERMATOPHAGOIDES PTERONYSSINUS GROUP 1 MAJOR ALLERGEN T CELL FUSION EPITOPE PEPTIDE VACCINE VECTOR BASED ON THE MHC II PATHWAY. Nutr Hosp. 2015;32:2274-9 pubmed publisher
    ..coli strain BL21 (DE3) and induced with IPTG, and the induced protein TAT-IhC-Der p1-3T was detected by SDS-PAGE...
  58. Li L, Lai C, Xuan X, Gao C, Li N. Simultaneous Determination of Hydrochlorothiazide and Losartan Potassium in Osmotic Pump Tablets by Microemulsion Liquid Chromatography. J Chromatogr Sci. 2016;54:1415-20 pubmed publisher
    ..The most effective MELC system had a mobile phase consisting of 95% (v/v) of 3.0% (w/w) SDS, 6.0% (w/w) n-butanol, 0.8% (w/w) n-octane, 90.2% (w/w) water and 5% (v/v) acetonitrile (pH 5). The flow rate was 1...
  59. Elbaz N, Khalil I, Abd Rabou A, El Sherbiny I. Chitosan-based nano-in-microparticle carriers for enhanced oral delivery and anticancer activity of propolis. Int J Biol Macromol. 2016;92:254-269 pubmed publisher
    ..The prepared SDs, NPs and NIMs were characterized using SEM, TEM, DLS, FTIR, DSC and UV-vis spectrophotometry...
  60. He Z, Tong C, Sheng L, Ma M, Cai Z. Monitoring glycation-induced structural and biofunctional changes in chicken immunoglobulin Y by different monosaccharides. Poult Sci. 2016;95:2715-2723 pubmed
    ..ultraviolet spectra, fluorescence spectra, circular dichroism spectra, Fourier transform infrared spectra, and SDS-PAGE and differential scanning calorimetry analysis...
  61. Berglund J, Szczepanski N, Penumarti A, Beavers A, Kesselring J, Orgel K, et al. Preparation and Analysis of Peanut Flour Used in Oral Immunotherapy Clinical Trials. J Allergy Clin Immunol Pract. 2017;5:1098-1104 pubmed publisher
    ..Densitometric scanning of Ara h 1 and Ara h 2 resolved on SDS-PAGE gels was used to assess allergen content in peanut flour extracts...
  62. Głab E, Wikiera B, Bieniasz J, Barg E. The Influence of GnRH Analog Therapy on Growth in Central Precocious Puberty. Adv Clin Exp Med. 2016;25:27-32 pubmed publisher
    ..There was a statistically significant difference in the PAH SDS before treatment between these two subgroups: Group I (-) 1.3 ± 1.8 vs. Group II (-) 0.14 ± 1...
  63. McLean H, Carriker C, Bordley W. Good to Great: Quality-Improvement Initiative Increases and Sustains Pediatric Health Care Worker Hand Hygiene Compliance. Hosp Pediatr. 2017;7:189-196 pubmed publisher
    ..Control charts of percentages of HCW HH compliance were constructed with 3-? (data within 3 SDs from a mean) control limits...
  64. Lobanova J, Gak E, Andreeva I, Rybak K, Krylov A, Mashko S. Complete nucleotide sequence and annotation of the temperate corynephage ?16 genome. Arch Virol. 2017;162:2489-2492 pubmed publisher
    ..2%) with protruding cohesive 3'-ends of 14 nt. Four major structural proteins were separated by SDS-PAGE and identified by peptide mass fingerprinting technique...
  65. Nasrollahzadeh F, Varidi M, Koocheki A, Hadizadeh F. Effect of microwave and conventional heating on structural, functional and antioxidant properties of bovine serum albumin-maltodextrin conjugates through Maillard reaction. Food Res Int. 2017;100:289-297 pubmed publisher
    ..Subsequently, SDS-PAGE, structural (surface hydrophobicity and circular dichroism), functional and antioxidant (DPPH radical ..
  66. Chappellaz M, Segboer H, Ulke Lemee A, Sutherland C, Chen H, MacDonald J. Quantitation of myosin regulatory light chain phosphorylation in biological samples with multiple reaction monitoring mass spectrometry. Biochim Biophys Acta Proteins Proteom. 2018;1866:608-616 pubmed publisher
    ..of the results for two quantification techniques indicate that the MRM-MS assay performs equally to Phos-tag SDS-PAGE for the determination of LC20 phosphorylation stoichiometry in arterial tissue samples...
  67. Chen M, Abele R, Tampe R. Peptides induce ATP hydrolysis at both subunits of the transporter associated with antigen processing. J Biol Chem. 2003;278:29686-92 pubmed
    ..of phosphate transition state analogs followed by photocross-linking, immunoprecipitation, and high resolution SDS-PAGE. Strikingly, trapping of both TAP subunits by beryllium fluoride is peptide-specific...
  68. Wang C, Zheng J, Sun J, Wang Y, Xia R, Yin Q, et al. Endogenous contrast T1rho cardiac magnetic resonance for myocardial fibrosis in hypertrophic cardiomyopathy patients. J Cardiol. 2015;66:520-6 pubmed publisher
    ..001), and T1rho-4-SD obtained the minimal mean difference with LGE (-3.1%; -15.2 to 9.1%), compared with other SDs. All the fibrotic sizes assessed by both methods correlated directly with LV maximal end-diastolic thickness (all p&..
  69. Gandreddi V, Kappala V, Zaveri K, Patnala K. Evaluating the role of a trypsin inhibitor from soap nut (Sapindus trifoliatus L. Var. Emarginatus) seeds against larval gut proteases, its purification and characterization. BMC Biochem. 2015;16:23 pubmed publisher
    ..SNTI exhibited a molecular weight of 29 kDa on SDS-PAGE, gel filtration and was negative to Periodic Acid Schiff's stain...
  70. Strasburger C, Vanuga P, Payer J, Pfeifer M, Popovic V, Bajnok L, et al. MOD-4023, a long-acting carboxy-terminal peptide-modified human growth hormone: results of a Phase 2 study in growth hormone-deficient adults. Eur J Endocrinol. 2017;176:283-294 pubmed publisher
    ..Insulin-like growth factor I (IGF-I) SDS levels were maintained within normal range. The 18.5% cohort was discontinued due to low efficacy...
  71. Zhang L, Chen H, Luo M, Zhang X, Deng M, Ma J, et al. Transposon insertion resulted in the silencing of Wx-B1n in Chinese wheat landraces. Theor Appl Genet. 2017;130:1321-1330 pubmed publisher
    ..of 53 Chinese wheat landraces was analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis; of these, 10 did not show the expression of Wx-A1 (four accession) ..
  72. Zhang M, Zhang Q, Zheng H, Wang X, Feng S, Tian W, et al. Clinical, demographic and psychological characteristics of infertile male smokers in Northeast China. J Int Med Res. 2016;44:75-80 pubmed publisher
    ..anxiety and depression were evaluated by the self-rating anxiety scale (SAS) and self-rating depression scale (SDS), respectively...
  73. Ferreira F, da Silva P, Soares T, Gonçalves Machado L, de Araújo L, da Silva T, et al. Evaluation of antimicrobial, cytotoxic, and hemolytic activities from venom of the spider Lasiodora sp. Toxicon. 2016;122:119-126 pubmed publisher
    ..Protein concentration of the Lasiodora sp. venom was 4.53 ± 0.38 mg/mL. SDS-PAGE showed proteins with molecular masses up to 75 kDa, some of which contained disulfide bridges...
  74. de Moura V, de Souza L, da Costa Guimarães N, Dos Santos I, de Almeida P, de Oliveira R, et al. The potential of aqueous extracts of Bellucia dichotoma Cogn. (Melastomataceae) to inhibit the biological activities of Bothrops atrox venom: A comparison of specimens collected in the states of Pará and Amazonas, Brazil. J Ethnopharmacol. 2017;196:168-177 pubmed publisher
    ..Interaction between BaV and the extracts was investigated using SDS-Page electrophoresis and Western blotting...
  75. Treumann A, Palmowski P, Tong W, Taggart J, Morrice N, Nicholas Europe Finner G, et al. Depletion of Myofibril-Associated Proteins Using Selective Protein Extraction as a Tool in Cardiac Proteomics. Methods Mol Biol. 2018;1788:1-9 pubmed publisher
    ..all theoretical mass spectra (SWATH), of guinea pig cardiac tissue prepared in two homogenization buffers: (1) An SDS-based buffer designed to extract "all" tissue proteins and (2) a long-established EDTA-containing buffer thought to ..
  76. Minelli A, Maserati E, Nicolis E, Zecca M, Sainati L, Longoni D, et al. The isochromosome i(7)(q10) carrying c.258+2t>c mutation of the SBDS gene does not promote development of myeloid malignancies in patients with Shwachman syndrome. Leukemia. 2009;23:708-11 pubmed publisher
    ..183_184TA>CT and c.258+2T>C) in exon 2 of the SBDS gene, localized on chromosome 7...
  77. Mamunur Rahman M, Azizur Rahman M, Maki T, Nishiuchi T, Asano T, Hasegawa H. A marine phytoplankton (Prymnesium parvum) up-regulates ABC transporters and several other proteins to acclimatize with Fe-limitation. Chemosphere. 2014;95:213-9 pubmed publisher
    ..In sodium dodecyl sulfate (SDS) gel electrophoresis, 7 proteins (16, 18, 32, 34, 75, 82, and 116 kDa) were highly expressed under Fe-deplete ..
  78. Vidanapathirana P, Hasan F, Mussio K, Pande A, Brands M, Siraj N, et al. Cationic ionic liquid surfactant-polyacrylamide gel electrophoresis for enhanced separation of acidic and basic proteins with single-step ribonuclease b glycoforms separation. J Chromatogr A. 2017;1515:245-251 pubmed publisher
    ..glycoform separation by use of ILS were compared with conventional non-ILS surfactants-PAGE: sodium dodecylsulfate (SDS)-PAGE, cetyltrimethylammonium bromide (CTAB)-PAGE, and benzyldimethyl-n-hexadecylammonium chloride (16-BAC)-PAGE...
  79. Boukid F, Prandi B, Buhler S, Sforza S. Effectiveness of Germination on Protein Hydrolysis as a Way To Reduce Adverse Reactions to Wheat. J Agric Food Chem. 2017;65:9854-9860 pubmed publisher
    ..General protein profile, as indicated by SDS-PAGE analysis, revealed that germination induced a relevant degradation in protein fraction...
  80. Bikos D, Mason T. Influence of Ionic Constituents and Electrical Conductivity on the Propagation of Charged Nanoscale Objects in Passivated Gel Electrophoresis. Electrophoresis. 2017;: pubmed publisher
    ..two different types of passivation agents: nonionic polyethylene glycol (PEG) and anionic sodium dodecyl sulfate (SDS)...
  81. Lu S, Edwards M. Molecular Characterization and Functional Analysis of PR-1-Like Proteins Identified from the Wheat Head Blight Fungus Fusarium graminearum. Phytopathology. 2018;108:510-520 pubmed publisher
    ..This study provides the first example that the F. graminearum-wheat interaction involves a pathogen-derived PR-1L protein that affects fungal virulence on the host. ..
  82. Wang Y, Yagasaki H, Hama A, Nishio N, Takahashi Y, Kojima S. Mutation of SBDS and SH2D1A is not associated with aplastic anemia in Japanese children. Haematologica. 2007;92:1573 pubmed
    ..Recently, genetic factors predisposing to aplastic anemia (AA) have been found in a few patients with apparently acquired AA. Here we present the genetic analysis of SBDS and SH2D1A in Japanese children with AA.
  83. Fujii K, Suzuki T, Mimura M, Uchida H. Psychological dependence on antidepressants in patients with panic disorder: a cross-sectional study. Int Clin Psychopharmacol. 2017;32:36-40 pubmed
    ..Assessments included the Japanese Versions of the Severity of Dependence Scale (SDS), the Self-Report Version of Panic Disorder Severity Scale (PDSS-SR), and the Quick Inventory of Depressive ..
  84. Matsumoto M, Awano H, Lee T, Takeshima Y, Matsuo M, Iijima K. Patients with Duchenne muscular dystrophy are significantly shorter than those with Becker muscular dystrophy, with the higher incidence of short stature in Dp71 mutated subgroup. Neuromuscul Disord. 2017;27:1023-1028 pubmed publisher
    ..42 BMD patients between 4 and 10 years of age was retrospectively examined using height standard deviation score (SDS). The mean height SDS of DMD was -1.08 SD that was significantly smaller than normal (p?<?0...
  85. Marinesco S, Ungvari Z, Galvan V. Age-related impairment of metabo-vascular coupling during cortical spreading depolarization. Am J Physiol Heart Circ Physiol. 2017;:ajpheart.00514.2017 pubmed publisher
    Cortical spreading depolarizations (SDs) may lead to long-lasting alterations in cerebral blood flow (CBF), which contribute to neuronal dysfunction and hypoxic tissue injury...
  86. Kampf J, Gundlach J, Herzberg C, Treffon K, Stülke J. Identification of c-di-AMP-Binding Proteins Using Magnetic Beads. Methods Mol Biol. 2017;1657:347-359 pubmed publisher
    ..In this approach, biotinylated c-di-AMP is coupled to Streptactin-covered beads. After protein separation using standard SDS-PAGE, the protein(s) of interest are identified by mass spectrometric analyses.
  87. Priji P, Sajith S, Faisal P, Benjamin S. Pseudomonas sp. BUP6 produces a thermotolerant alkaline lipase with trans-esterification efficiency in producing biodiesel. 3 Biotech. 2017;7:369 pubmed publisher
    ..The apparent molecular weight of this lipase on SDS-PAGE was 35 kDa, the identity of which was further confirmed by MALDI-TOF/MS...
  88. Tanaka H, Sasaki H. Cognitive impairment with interferon treatment in patients with chronic hepatitis C. Biomed Res. 2017;38:371-374 pubmed publisher
    ..In addition, 10 patients of them completed the self-rating depression scale (SDS). P300 latency was significantly prolonged at all points of measurement during IFN treatment...
  89. Rujkijyanont P, Watanabe K, Ambekar C, Wang H, Schimmer A, Beyene J, et al. SBDS-deficient cells undergo accelerated apoptosis through the Fas-pathway. Haematologica. 2008;93:363-71 pubmed publisher
    ..Ninety percent of the patients have mutations in the Shwachman-Bodian-Diamond syndrome gene (SBDS). The relationship between SBDS and cell survival is unknown...
  90. In K, Zaini M, Müller C, Warren A, von Lindern M, Calkhoven C. Shwachman-Bodian-Diamond syndrome (SBDS) protein deficiency impairs translation re-initiation from C/EBPα and C/EBPβ mRNAs. Nucleic Acids Res. 2016;44:4134-46 pubmed publisher
    Mutations in the Shwachman-Bodian-Diamond Syndrome (SBDS) gene cause Shwachman-Diamond Syndrome (SDS), a rare congenital disease characterized by bone marrow failure with neutropenia, exocrine pancreatic dysfunction and skeletal ..
  91. Croonen E, Essink M, van der Burgt I, Draaisma J, Noordam C, Nijhuis van der Sanden M. Motor performance in children with Noonan syndrome. Am J Med Genet A. 2017;173:2335-2345 pubmed publisher
    ..with Noonan syndrome (12 females, mean age 9 years 4 months, range 6 years 1 month to 11 years and 11 months, SDS 1 year and 11 months)...
  92. Elmas B, Karacan M, Dervişoğlu P, Kosecik M, İşgüven Ş, Bal C. Dynamic thiol/disulphide homeostasis as a novel indicator of oxidative stress in obese children and its relationship with inflammatory-cardiovascular markers. Anatol J Cardiol. 2017;18:361-369 pubmed publisher
    ..Correlation analyses of thiol/disulphide homeostasis parameters with body mass index standard deviation scores (BMI SDS) and inflammatory and cardiovascular markers were performed...