RUNX2

Summary

Gene Symbol: RUNX2
Description: runt related transcription factor 2
Alias: AML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA, runt-related transcription factor 2, PEA2-alpha A, PEBP2-alpha A, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, acute myeloid leukemia 3 protein, core-binding factor, runt domain, alpha subunit 1, oncogene AML-3, osteoblast-specific transcription factor 2, polyomavirus enhancer-binding protein 2 alpha A subunit
Species: human
Products:     RUNX2

Top Publications

  1. Inman C, Li N, Shore P. Oct-1 counteracts autoinhibition of Runx2 DNA binding to form a novel Runx2/Oct-1 complex on the promoter of the mammary gland-specific gene beta-casein. Mol Cell Biol. 2005;25:3182-93 pubmed
    The transcription factor Runx2 is essential for the expression of a number of bone-specific genes and is primarily considered a master regulator of bone development...
  2. Barnes G, Hebert K, Kamal M, Javed A, Einhorn T, Lian J, et al. Fidelity of Runx2 activity in breast cancer cells is required for the generation of metastases-associated osteolytic disease. Cancer Res. 2004;64:4506-13 pubmed
    ..We demonstrated recently that breast cancer cells express the Runx2 transcription factor, essential for bone formation and a regulator of skeletal homeostasis...
  3. Zhang Y, Yasui N, Ito K, Huang G, Fujii M, Hanai J, et al. A RUNX2/PEBP2alpha A/CBFA1 mutation displaying impaired transactivation and Smad interaction in cleidocranial dysplasia. Proc Natl Acad Sci U S A. 2000;97:10549-54 pubmed
    ..autosomal-dominant human bone disease, is thought to be caused by heterozygous mutations in runt-related gene 2 (RUNX2)/polyomavirus enhancer binding protein 2alphaA (PEBP2alphaA)/core-binding factor A1 (CBFA1)...
  4. Ermakov S, Malkin I, Keter M, Kobyliansky E, Livshits G. Family-based association study of polymorphisms in the RUNX2 locus with hand bone length and hand BMD. Ann Hum Genet. 2008;72:510-8 pubmed publisher
    ..to test the hypothesis of association of radiographic hand bone length (BL) and BMD with polymorphisms in the RUNX2 locus. Four SNPs linked to the two RUNX2 promoters were genotyped in 212 nuclear Caucasian families...
  5. Sevetson B, Taylor S, Pan Y. Cbfa1/RUNX2 directs specific expression of the sclerosteosis gene (SOST). J Biol Chem. 2004;279:13849-58 pubmed
    ..b>Cbfa1/RUNX2 is a key transcriptional regulator of osteoblast function...
  6. Sierra J, Villagra A, Paredes R, Cruzat F, Gutierrez S, Javed A, et al. Regulation of the bone-specific osteocalcin gene by p300 requires Runx2/Cbfa1 and the vitamin D3 receptor but not p300 intrinsic histone acetyltransferase activity. Mol Cell Biol. 2003;23:3339-51 pubmed
    ..In osteoblastic cells, transcription of the bone-specific osteocalcin (OC) gene is principally regulated by the Runx2/Cbfa1 transcription factor and is stimulated in response to vitamin D(3) via the vitamin D(3) receptor complex...
  7. Jun J, Yoon W, Seo S, Woo K, Kim G, Ryoo H, et al. BMP2-activated Erk/MAP kinase stabilizes Runx2 by increasing p300 levels and histone acetyltransferase activity. J Biol Chem. 2010;285:36410-9 pubmed publisher
    b>Runx2 is a critical transcription factor for osteoblast differentiation. Regulation of Runx2 expression levels and transcriptional activity is important for bone morphogenetic protein (BMP)-induced osteoblast differentiation...
  8. Barnes G, Javed A, Waller S, Kamal M, Hebert K, Hassan M, et al. Osteoblast-related transcription factors Runx2 (Cbfa1/AML3) and MSX2 mediate the expression of bone sialoprotein in human metastatic breast cancer cells. Cancer Res. 2003;63:2631-7 pubmed
    ..Our results demonstrate that Runx2 is ectopically expressed in breast cancer cells and that one isoform of Runx2 can activate bsp expression in these ..
  9. Yoshida T, Kanegane H, Osato M, Yanagida M, Miyawaki T, Ito Y, et al. Functional analysis of RUNX2 mutations in Japanese patients with cleidocranial dysplasia demonstrates novel genotype-phenotype correlations. Am J Hum Genet. 2002;71:724-38 pubmed
    ..heritable skeletal disease caused by heterozygous mutations in the osteoblast-specific transcription factor RUNX2. We have performed mutational analysis of RUNX2 on 24 unrelated patients with CCD...

More Information

Publications108 found, 100 shown here

  1. Pratap J, Javed A, Languino L, Van Wijnen A, Stein J, Stein G, et al. The Runx2 osteogenic transcription factor regulates matrix metalloproteinase 9 in bone metastatic cancer cells and controls cell invasion. Mol Cell Biol. 2005;25:8581-91 pubmed
    The Runx2 (Cbfa1/AML3) transcription factor and matrix metalloproteinase 9 (MMP9) are key regulators of growth plate maturation and bone formation...
  2. Chua C, Chiu Y, Yuen H, Chan K, Man K, Wang X, et al. Suppression of androgen-independent prostate cancer cell aggressiveness by FTY720: validating Runx2 as a potential antimetastatic drug screening platform. Clin Cancer Res. 2009;15:4322-35 pubmed publisher
    ..In the present study, we aimed to first verify the role of Runx2 in prostate cancer progression and metastasis, and, subsequently, assessed if FTY720 could modulate Runx2 ..
  3. Jones D, Wein M, Oukka M, Hofstaetter J, Glimcher M, Glimcher L. Regulation of adult bone mass by the zinc finger adapter protein Schnurri-3. Science. 2006;312:1223-7 pubmed
    ..Shn3 was found to control protein levels of Runx2, the principal transcriptional regulator of osteoblast differentiation, by promoting its degradation through ..
  4. Pratap J, Imbalzano K, Underwood J, Cohet N, Gokul K, Akech J, et al. Ectopic runx2 expression in mammary epithelial cells disrupts formation of normal acini structure: implications for breast cancer progression. Cancer Res. 2009;69:6807-14 pubmed publisher
    The transcription factor Runx2 is highly expressed in breast cancer cells compared with mammary epithelial cells and contributes to metastasis...
  5. van der Deen M, Akech J, Lapointe D, Gupta S, Young D, Montecino M, et al. Genomic promoter occupancy of runt-related transcription factor RUNX2 in Osteosarcoma cells identifies genes involved in cell adhesion and motility. J Biol Chem. 2012;287:4503-17 pubmed publisher
    Runt-related transcription factors (RUNX1, RUNX2, and RUNX3) are key lineage-specific regulators of progenitor cell growth and differentiation but also function pathologically as cancer genes that contribute to tumorigenesis...
  6. Drissi H, Luc Q, Shakoori R, Chuva De Sousa Lopes S, Choi J, Terry A, et al. Transcriptional autoregulation of the bone related CBFA1/RUNX2 gene. J Cell Physiol. 2000;184:341-50 pubmed
    The runt related transcription factor CBFA1 (AML3/PEBP2alphaA/RUNX2) regulates expression of several bone- and cartilage-related genes and is required for bone formation in vivo...
  7. Sase T, Suzuki T, Miura K, Shiiba K, Sato I, Nakamura Y, et al. Runt-related transcription factor 2 in human colon carcinoma: a potent prognostic factor associated with estrogen receptor. Int J Cancer. 2012;131:2284-93 pubmed publisher
    Runt-related transcription factor 2 (RUNX2) belongs to the RUNX family of heterodimeric transcription factors, and is mainly associated with osteogenesis...
  8. Jimenez M, Balbin M, Lopez J, Alvarez J, Komori T, Lopez Otin C. Collagenase 3 is a target of Cbfa1, a transcription factor of the runt gene family involved in bone formation. Mol Cell Biol. 1999;19:4431-42 pubmed
    ..In this work, we have evaluated the possibility that Cbfa1 (core binding factor 1), a transcription factor playing a major role in the expression of osteoblastic specific ..
  9. Shen R, Chen M, Wang Y, Kaneki H, Xing L, O Keefe R, et al. Smad6 interacts with Runx2 and mediates Smad ubiquitin regulatory factor 1-induced Runx2 degradation. J Biol Chem. 2006;281:3569-76 pubmed
    b>Runx2 is a bone-specific transcription factor that plays a critical role in bone development, postnatal bone formation, and chondrocyte maturation. The protein levels of Runx2 are regulated by the ubiquitin-proteasome pathway...
  10. Kim B, Kim H, Park H, Kim Y, Yoon W, Lee S, et al. Runx2 phosphorylation induced by fibroblast growth factor-2/protein kinase C pathways. Proteomics. 2006;6:1166-74 pubmed
    b>Runx2 is a key transcription factor in osteoblast differentiation, and its activity is regulated by fibroblast growth factors (FGFs)...
  11. Lee H, Koh J, Hwang J, Choi K, Lee S, Park E, et al. Association of a RUNX2 promoter polymorphism with bone mineral density in postmenopausal Korean women. Calcif Tissue Int. 2009;84:439-45 pubmed publisher
    ..Bone mineral density (BMD) is a major determinant of bone strength. RUNX2 is an osteoblast-specific transcription factor involved in osteoblast differentiation and ossification...
  12. Ning Y, Robins D. AML3/CBFalpha1 is required for androgen-specific activation of the enhancer of the mouse sex-limited protein (Slp) gene. J Biol Chem. 1999;274:30624-30 pubmed
    ..by a high affinity binding site specific for AML/CBFalpha and was specifically supershifted by an antibody to AML3/CBFalpha1, placing factor B within the AML3/CBFalpha1 subclass...
  13. Mundlos S, Otto F, Mundlos C, Mulliken J, Aylsworth A, Albright S, et al. Mutations involving the transcription factor CBFA1 cause cleidocranial dysplasia. Cell. 1997;89:773-9 pubmed
    ..In some families, the phenotype segregates with deletions resulting in heterozygous loss of CBFA1, a member of the runt family of transcription factors...
  14. Pockwinse S, Rajgopal A, Young D, Mujeeb K, Nickerson J, Javed A, et al. Microtubule-dependent nuclear-cytoplasmic shuttling of Runx2. J Cell Physiol. 2006;206:354-62 pubmed
    ..lineages and have been linked to human cancers including acute myelogenous leukemia (RUNX1), as well as breast (RUNX2) and gastric cancers (RUNX3)...
  15. Quack I, Vonderstrass B, Stock M, Aylsworth A, Becker A, Brueton L, et al. Mutation analysis of core binding factor A1 in patients with cleidocranial dysplasia. Am J Hum Genet. 1999;65:1268-78 pubmed
    ..We recently demonstrated that mutations in the transcription factor CBFA1, on chromosome 6p21, are associated with CCD...
  16. Otto F, Kanegane H, Mundlos S. Mutations in the RUNX2 gene in patients with cleidocranial dysplasia. Hum Mutat. 2002;19:209-16 pubmed
    ..The locus for this disease was mapped to chromosome 6p21. RUNX2 is a member of the runt family of transcription factors and its expression is restricted to developing osteoblasts ..
  17. Bustamante M, Nogues X, Agueda L, Jurado S, Wesselius A, Caceres E, et al. Promoter 2 -1025 T/C polymorphism in the RUNX2 gene is associated with femoral neck bmd in Spanish postmenopausal women. Calcif Tissue Int. 2007;81:327-32 pubmed
    Stimulation of bone formation is a key therapeutic target in osteoporosis. Runx2 is a runt domain transcription factor essential to osteoblast differentiation, bone remodeling, and fracture healing...
  18. Zhang H, Pan Y, Zheng L, Choe C, Lindgren B, Jensen E, et al. FOXO1 inhibits Runx2 transcriptional activity and prostate cancer cell migration and invasion. Cancer Res. 2011;71:3257-67 pubmed publisher
    ..The transcription factor Runx2 plays an important role in bone formation and prostate cancer cell migration, invasion, and metastasis...
  19. Pande S, Browne G, Padmanabhan S, Zaidi S, Lian J, Van Wijnen A, et al. Oncogenic cooperation between PI3K/Akt signaling and transcription factor Runx2 promotes the invasive properties of metastatic breast cancer cells. J Cell Physiol. 2013;228:1784-92 pubmed publisher
    ..Our study shows that Akt phosphorylates the cancer-related transcription factor Runx2 resulting in stimulated DNA binding of the purified recombinant protein in vitro...
  20. Thomas D, Johnson S, Sims N, Trivett M, Slavin J, Rubin B, et al. Terminal osteoblast differentiation, mediated by runx2 and p27KIP1, is disrupted in osteosarcoma. J Cell Biol. 2004;167:925-34 pubmed
    ..The function of runx2, a master regulator of osteoblast differentiation belonging to the runt family of tumor suppressor genes, is ..
  21. Zhang H, Jin L, Stilling G, Ruebel K, Coonse K, Tanizaki Y, et al. RUNX1 and RUNX2 upregulate Galectin-3 expression in human pituitary tumors. Endocrine. 2009;35:101-11 pubmed publisher
    ..Two binding sites for RUNX1 and one binding site for RUNX2 were identified in the LGALS3 promoter region...
  22. Tandon M, Gokul K, Ali S, Chen Z, Lian J, Stein G, et al. Runx2 mediates epigenetic silencing of the bone morphogenetic protein-3B (BMP-3B/GDF10) in lung cancer cells. Mol Cancer. 2012;11:27 pubmed publisher
    The Runt-related transcription factor Runx2 is essential for bone development but is also implicated in progression of several cancers of breast, prostate and bone, where it activates cancer-related genes and promotes invasive properties...
  23. Rajgopal A, Young D, Mujeeb K, Stein J, Lian J, Van Wijnen A, et al. Mitotic control of RUNX2 phosphorylation by both CDK1/cyclin B kinase and PP1/PP2A phosphatase in osteoblastic cells. J Cell Biochem. 2007;100:1509-17 pubmed
    Skeletal development and osteoblast maturation require the phenotype promoting activity of the transcription factor RUNX2, which controls both cell growth and differentiation in osteoblasts...
  24. Bergwitz C, Prochnau A, Mayr B, Kramer F, Rittierodt M, Berten H, et al. Identification of novel CBFA1/RUNX2 mutations causing cleidocranial dysplasia. J Inherit Metab Dis. 2001;24:648-56 pubmed
    Core binding factor A1 (CBFA1/RUNX2) is a runt-like transcription factor essential for osteoblast differentiation...
  25. Westendorf J, Zaidi S, Cascino J, Kahler R, Van Wijnen A, Lian J, et al. Runx2 (Cbfa1, AML-3) interacts with histone deacetylase 6 and represses the p21(CIP1/WAF1) promoter. Mol Cell Biol. 2002;22:7982-92 pubmed
    b>Runx2 (Cbfa1, AML-3) is multifunctional transcription factor that is essential for osteoblast development...
  26. Wang G, Sun R, Song F. A novel RUNX2 mutation (T420I) in Chinese patients with cleidocranial dysplasia. Genet Mol Res. 2010;9:41-7 pubmed publisher
    ..dysplasia (CCD) is an autosomal-dominant heritable skeletal disease caused by heterozygous mutations in the RUNX2 gene...
  27. Baniwal S, Khalid O, Gabet Y, Shah R, Purcell D, Mav D, et al. Runx2 transcriptome of prostate cancer cells: insights into invasiveness and bone metastasis. Mol Cancer. 2010;9:258 pubmed publisher
    ..b>Runx2, an osteoblast master transcription factor, is aberrantly expressed in PCa cells, and promotes their metastatic ..
  28. Cunningham M, Seto M, Hing A, Bull M, Hopkin R, Leppig K. Cleidocranial dysplasia with severe parietal bone dysplasia: C-terminal RUNX2 mutations. Birth Defects Res A Clin Mol Teratol. 2006;76:78-85 pubmed
    ..CCD is caused by mutations in the transcription factor RUNX2, which is known to function as a major regulator of bone differentiation...
  29. Higashikawa A, Saito T, Ikeda T, Kamekura S, Kawamura N, Kan A, et al. Identification of the core element responsive to runt-related transcription factor 2 in the promoter of human type X collagen gene. Arthritis Rheum. 2009;60:166-78 pubmed publisher
    ..Studies on molecular networks related to RUNX-2 and the HY box will lead to treatments of skeletal growth retardation, bone fracture, and OA. ..
  30. Chimge N, Baniwal S, Luo J, Coetzee S, Khalid O, Berman B, et al. Opposing effects of Runx2 and estradiol on breast cancer cell proliferation: in vitro identification of reciprocally regulated gene signature related to clinical letrozole responsiveness. Clin Cancer Res. 2012;18:901-11 pubmed publisher
    To assess the clinical significance of the interaction between estrogen and Runx2 signaling, previously shown in vitro...
  31. Vaughan T, Pasco J, Kotowicz M, Nicholson G, Morrison N. Alleles of RUNX2/CBFA1 gene are associated with differences in bone mineral density and risk of fracture. J Bone Miner Res. 2002;17:1527-34 pubmed
    The aim of this study was to determine if DNA polymorphism within runt-related gene 2 (RUNX2)/core binding factor A1 (CBFA1) is related to bone mineral density (BMD)...
  32. Lim M, Zhong C, Yang S, Bell A, Cohen M, Roy Burman P. Runx2 regulates survivin expression in prostate cancer cells. Lab Invest. 2010;90:222-33 pubmed publisher
    ..Here, for the first time, we identify Runx2 as a key regulator of survivin transcription...
  33. Qiao M, Shapiro P, Fosbrink M, Rus H, Kumar R, Passaniti A. Cell cycle-dependent phosphorylation of the RUNX2 transcription factor by cdc2 regulates endothelial cell proliferation. J Biol Chem. 2006;281:7118-28 pubmed
    b>RUNX2 is a member of the runt family of DNA-binding transcription factors. RUNX2 mediates endothelial cell migration and invasion during tumor angiogenesis and is expressed in metastatic breast and prostate tumors...
  34. Nathan S, Pereira B, Zhou Y, Gupta A, Dombrowski C, Soong R, et al. Elevated expression of Runx2 as a key parameter in the etiology of osteosarcoma. Mol Biol Rep. 2009;36:153-8 pubmed publisher
    ..Molecular analysis reveals OS1 cells express the pocket protein pRB and the runt-related transcription factor Runx2. Strikingly, Runx2 is expressed at higher levels in OS1 cells than in human fetal osteoblasts...
  35. Hsu Y, Huang M, Yang C, Hung J, Wu L, Kuo P. Lung tumor-associated osteoblast-derived bone morphogenetic protein-2 increased epithelial-to-mesenchymal transition of cancer by Runx2/Snail signaling pathway. J Biol Chem. 2011;286:37335-46 pubmed publisher
    ..of lung cancer migration by BMP-2 is associated with increased ERK and p38 activation and the up-regulation of Runx2 and Snail...
  36. Lee B, Thirunavukkarasu K, Zhou L, Pastore L, Baldini A, Hecht J, et al. Missense mutations abolishing DNA binding of the osteoblast-specific transcription factor OSF2/CBFA1 in cleidocranial dysplasia. Nat Genet. 1997;16:307-10 pubmed
    ..CCD has been mapped to chromosome 6p21, where CBFA1, a gene encoding OSF2/CBFA1, a transcriptional activator of osteoblast differentiation, has been localized...
  37. Park O, Kim H, Woo K, Baek J, Ryoo H. FGF2-activated ERK mitogen-activated protein kinase enhances Runx2 acetylation and stabilization. J Biol Chem. 2010;285:3568-74 pubmed publisher
    b>Runx2 is a key transcription factor regulating osteoblast differentiation and skeletal morphogenesis, and FGF2 is one of the most important regulators of skeletal development...
  38. Ogawa E, Maruyama M, Kagoshima H, Inuzuka M, Lu J, Satake M, et al. PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene. Proc Natl Acad Sci U S A. 1993;90:6859-63 pubmed
    ..The major species of PEBP2 alpha mRNA was expressed in T-cell lines but not in B-cell lines tested. Evidence indicated that PEBP2 functions as a transcriptional activator and is involved in regulation of T-cell-specific gene expression. ..
  39. Ducy P, Zhang R, Geoffroy V, Ridall A, Karsenty G. Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell. 1997;89:747-54 pubmed
    ..We have now cloned the cDNA encoding Osf2/Cbfa1, the protein that binds to OSE2...
  40. Tessa A, Salvi S, Casali C, Garavelli L, Digilio M, Dotti M, et al. Six novel mutations of the RUNX2 gene in Italian patients with cleidocranial dysplasia. Hum Mutat. 2003;22:104 pubmed
    ..We identified ten heterozygous base changes in the RUNX2 gene, including six novel mutations [c.522insA, c.389G>A (W130X), c.662T>G (V221G), IVS2+T>A, c...
  41. Ohyama Y, Tanaka T, Shimizu T, Matsui H, Sato H, Koitabashi N, et al. Runx2/Smad3 complex negatively regulates TGF-?-induced connective tissue growth factor gene expression in vascular smooth muscle cells. J Atheroscler Thromb. 2012;19:23-35 pubmed
    ..We previously showed that Runx2, a key transcription factor in osteoblast differentiation, regulates osteogenic conversion and dedifferentiation ..
  42. Otto F, Thornell A, Crompton T, Denzel A, Gilmour K, Rosewell I, et al. Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development. Cell. 1997;89:765-71 pubmed
    We have generated Cbfa1-deficient mice. Homozygous mutants die of respiratory failure shortly after birth. Analysis of their skeletons revealed an absence of osteoblasts and bone...
  43. Yagi R, Chen L, Shigesada K, Murakami Y, Ito Y. A WW domain-containing yes-associated protein (YAP) is a novel transcriptional co-activator. EMBO J. 1999;18:2551-62 pubmed
    ..These results indicate that the PY motif is a novel transcription activation domain that functions by recruiting YAP as a strong transcription activator to target genes. ..
  44. Zhou G, Chen Y, Zhou L, Thirunavukkarasu K, Hecht J, Chitayat D, et al. CBFA1 mutation analysis and functional correlation with phenotypic variability in cleidocranial dysplasia. Hum Mol Genet. 1999;8:2311-6 pubmed
    ..is a dominantly inherited skeletal dysplasia caused by mutations in the osteoblast-specific transcription factor CBFA1. To correlate CBFA1 mutations in different functional domains with the CCD clinical spectrum, we studied 26 ..
  45. Zhang Y, Yasui N, Kakazu N, Abe T, Takada K, Imai S, et al. PEBP2alphaA/CBFA1 mutations in Japanese cleidocranial dysplasia patients. Gene. 2000;244:21-8 pubmed
    ..dominant human bone disease whose genetic locus has been located on chromosome 6p21, where the PEBP2alphaA/CBFA1 gene essential for osteogenesis also maps...
  46. Zaidi S, Sullivan A, Van Wijnen A, Stein J, Stein G, Lian J. Integration of Runx and Smad regulatory signals at transcriptionally active subnuclear sites. Proc Natl Acad Sci U S A. 2002;99:8048-53 pubmed
    ..Thus, Runx-mediated intranuclear targeting of Smads is critical for the integration of two distinct pathways essential for fetal development. ..
  47. Vega R, Matsuda K, Oh J, Barbosa A, Yang X, Meadows E, et al. Histone deacetylase 4 controls chondrocyte hypertrophy during skeletogenesis. Cell. 2004;119:555-66 pubmed
    ..chondrocyte hypertrophy and endochondral bone formation by interacting with and inhibiting the activity of Runx2, a transcription factor necessary for chondrocyte hypertrophy...
  48. Phimphilai M, Zhao Z, Boules H, Roca H, Franceschi R. BMP signaling is required for RUNX2-dependent induction of the osteoblast phenotype. J Bone Miner Res. 2006;21:637-46 pubmed
    b>RUNX2 expression in mesenchymal cells induces osteoblast differentiation and bone formation...
  49. Jeon E, Lee K, Choi N, Lee M, Kim H, Jin Y, et al. Bone morphogenetic protein-2 stimulates Runx2 acetylation. J Biol Chem. 2006;281:16502-11 pubmed
    Runx2/Cbfa1/Pebp2aA is a global regulator of osteogenesis and is crucial for regulating the expression of bone-specific genes. Runx2 is a major target of the bone morphogenetic protein (BMP) pathway...
  50. Young D, Hassan M, Yang X, Galindo M, Javed A, Zaidi S, et al. Mitotic retention of gene expression patterns by the cell fate-determining transcription factor Runx2. Proc Natl Acad Sci U S A. 2007;104:3189-94 pubmed
    ..Here we show that Runx2 protein is stable during cell division and remains associated with chromosomes during mitosis through sequence-..
  51. D Souza D, Salib M, Bennett J, Mochin Peters M, Asrani K, Goldblum S, et al. Hyperglycemia regulates RUNX2 activation and cellular wound healing through the aldose reductase polyol pathway. J Biol Chem. 2009;284:17947-55 pubmed publisher
    ..The RUNX2 transcription factor, which is repressed in diabetic animals, promotes vascular endothelial cell (EC) migration, ..
  52. Sun X, Wei L, Chen Q, Terek R. HDAC4 represses vascular endothelial growth factor expression in chondrosarcoma by modulating RUNX2 activity. J Biol Chem. 2009;284:21881-90 pubmed publisher
    ..selective growth advantage are often recapitulated in tumors, we investigated the regulation of VEGF by HDAC4 and Runx2 in chondrosarcoma...
  53. Sadikovic B, Thorner P, Chilton MacNeill S, Martin J, Cervigne N, Squire J, et al. Expression analysis of genes associated with human osteosarcoma tumors shows correlation of RUNX2 overexpression with poor response to chemotherapy. BMC Cancer. 2010;10:202 pubmed publisher
    ..Recent discoveries have highlighted a potential role of a number of genes including: RECQL4, DOCK5, SPP1, RUNX2, RB1, CDKN1A, P53, IBSP, LSAMP, MYC, TNFRSF1B, BMP2, HISTH2BE, FOS, CCNB1, and CDC5L...
  54. Li H, Zhou R, Zhang G, Xu J. Clinical significance of RUNX2 expression in patients with nonsmall cell lung cancer: a 5-year follow-up study. Tumour Biol. 2013;34:1807-12 pubmed publisher
    ..b>RUNX2 expression was examined at mRNA and protein levels by real-time quantitative reverse transcription polymerase ..
  55. Gutierrez S, Javed A, Tennant D, van Rees M, Montecino M, Stein G, et al. CCAAT/enhancer-binding proteins (C/EBP) beta and delta activate osteocalcin gene transcription and synergize with Runx2 at the C/EBP element to regulate bone-specific expression. J Biol Chem. 2002;277:1316-23 pubmed
    ..element in the proximal promoter of the rat osteocalcin gene, which resides in close proximity to a Runx2 (Cbfa1) element, essential for tissue-specific activation...
  56. Javed A, Barnes G, Pratap J, Antkowiak T, Gerstenfeld L, Van Wijnen A, et al. Impaired intranuclear trafficking of Runx2 (AML3/CBFA1) transcription factors in breast cancer cells inhibits osteolysis in vivo. Proc Natl Acad Sci U S A. 2005;102:1454-9 pubmed
    ..We have previously shown that aberrant expression of the Runx2 DNA binding domain in metastatic breast cancer cells can prevent production of osteolytic lesions in bone...
  57. Blyth K, Cameron E, Neil J. The RUNX genes: gain or loss of function in cancer. Nat Rev Cancer. 2005;5:376-87 pubmed
    ..have such an ambiguous role in cancer? How and where do these genes impinge on the pathways that regulate growth control and differentiation? And what is the evidence for a wider role for the RUNX genes in non-haematopoietic cancers? ..
  58. Ermakov S, Malkin I, Kobyliansky E, Livshits G. Variation in femoral length is associated with polymorphisms in RUNX2 gene. Bone. 2006;38:199-205 pubmed
    ..The major objective of the present study was to test hypothesis of possible association between three RUNX2 SNP polymorphisms (rs2819858, rs1406846, rs2819854) and anthropometrical femoral length (FEML)...
  59. Cotter E, Malizia A, Chew N, Powderly W, Doran P. HIV proteins regulate bone marker secretion and transcription factor activity in cultured human osteoblasts with consequent potential implications for osteoblast function and development. AIDS Res Hum Retroviruses. 2007;23:1521-30 pubmed
    ..HIV proteins can potentially modulate OB development and function in vitro via modulation of bone maker secretion and RUNX-2 and PPARgamma transcription factor activity. ..
  60. Makita N, Suzuki M, Asami S, Takahata R, Kohzaki D, Kobayashi S, et al. Two of four alternatively spliced isoforms of RUNX2 control osteocalcin gene expression in human osteoblast cells. Gene. 2008;413:8-17 pubmed publisher
    b>Runx2 is a Runt domain transcription factor that transcriptionally regulates osteoblast differentiation and bone formation...
  61. Endo T, Ohta K, Kobayashi T. Expression and function of Cbfa-1/Runx2 in thyroid papillary carcinoma cells. J Clin Endocrinol Metab. 2008;93:2409-12 pubmed publisher
    ..from BHP18-21 cells and FRTL-5 cells demonstrated that cells of thyroid epithelial origin expressed Cbfa-1/Runx2, the main transcription factor for the expression of osteocalcin. When we transfected pcDNA3...
  62. Pratap J, Wixted J, Gaur T, Zaidi S, Dobson J, Gokul K, et al. Runx2 transcriptional activation of Indian Hedgehog and a downstream bone metastatic pathway in breast cancer cells. Cancer Res. 2008;68:7795-802 pubmed publisher
    b>Runx2, required for bone formation, is ectopically expressed in breast cancer cells...
  63. Das K, Leong D, Gupta A, Shen L, Putti T, Stein G, et al. Positive association between nuclear Runx2 and oestrogen-progesterone receptor gene expression characterises a biological subtype of breast cancer. Eur J Cancer. 2009;45:2239-48 pubmed publisher
    The runt-related transcription factor, Runx2 may have an oncogenic role in mediating metastatic events in breast cancer, but whether Runx2 has a role in the early phases of breast cancer development is not clear...
  64. Slattery M, Lundgreen A, Herrick J, Caan B, Potter J, Wolff R. Associations between genetic variation in RUNX1, RUNX2, RUNX3, MAPK1 and eIF4E and riskof colon and rectal cancer: additional support for a TGF-?-signaling pathway. Carcinogenesis. 2011;32:318-26 pubmed publisher
    ..We evaluated genetic variation in RUNX1 (40 tagSNPs), RUNX2 (19 tagSNPs), RUNX3 (9 tagSNPs), MAPK1 (6 tagSNPs), eIF4E (3 tagSNPs), eIF4EBP2 (2 tagSNP) and eIF4EBP3 (2 tagSNPs)..
  65. Dalle Carbonare L, Innamorati G, Valenti M. Transcription factor Runx2 and its application to bone tissue engineering. Stem Cell Rev. 2012;8:891-7 pubmed publisher
    Cbfa1/Runx2 is a bone transcription factor homologous to the Drosophila protein, Runt. Runx2 is a master gene that encodes for a protein involved in the osteogenic differentiation process from mesenchymal precursors...
  66. Lee S, Che X, Jeong J, Choi J, Lee Y, Lee Y, et al. Runx2 protein stabilizes hypoxia-inducible factor-1? through competition with von Hippel-Lindau protein (pVHL) and stimulates angiogenesis in growth plate hypertrophic chondrocytes. J Biol Chem. 2012;287:14760-71 pubmed publisher
    ..Here, we investigated the cross-talk between HIF-1? and Runt-related transcription factor 2 (Runx2) in the growth plate...
  67. Morrison N, Stephens A, Osato M, Polly P, Tan T, Yamashita N, et al. Glutamine repeat variants in human RUNX2 associated with decreased femoral neck BMD, broadband ultrasound attenuation and target gene transactivation. PLoS ONE. 2012;7:e42617 pubmed publisher
    b>RUNX2 is an essential transcription factor required for skeletal development and cartilage formation...
  68. Li W, Xu S, Lin S, Zhao W. Overexpression of runt-related transcription factor-2 is associated with advanced tumor progression and poor prognosis in epithelial ovarian cancer. J Biomed Biotechnol. 2012;2012:456534 pubmed publisher
    ..b>RUNX2 protein expression and its subcellular localization were detected by immunohistochemistry in 116 patients with EOC...
  69. Levanon D, Negreanu V, Bernstein Y, Bar Am I, Avivi L, Groner Y. AML1, AML2, and AML3, the human members of the runt domain gene-family: cDNA structure, expression, and chromosomal localization. Genomics. 1994;23:425-32 pubmed
    cDNAs corresponding to three human runt domain containing genes, AML1, AML2, and AML3, were isolated and characterized...
  70. Schroeder T, Kahler R, Li X, Westendorf J. Histone deacetylase 3 interacts with runx2 to repress the osteocalcin promoter and regulate osteoblast differentiation. J Biol Chem. 2004;279:41998-2007 pubmed
    The Runt domain transcription factor Runx2 (AML-3, and Cbfa1) is essential for osteoblast development, differentiation, and bone formation...
  71. Young D, Hassan M, Pratap J, Galindo M, Zaidi S, Lee S, et al. Mitotic occupancy and lineage-specific transcriptional control of rRNA genes by Runx2. Nature. 2007;445:442-6 pubmed
    ..Runt-related transcription factors Runx1, Runx2 and Runx3 establish and maintain cell identity, and convey phenotypic information through successive cell ..
  72. Jonason J, Xiao G, Zhang M, Xing L, Chen D. Post-translational Regulation of Runx2 in Bone and Cartilage. J Dent Res. 2009;88:693-703 pubmed publisher
    The Runx2 gene product is essential for mammalian bone development. In humans, Runx2 haploinsufficiency results in cleidocranial dysplasia, a skeletal disorder characterized by bone and dental abnormalities...
  73. Onodera Y, Miki Y, Suzuki T, Takagi K, Akahira J, Sakyu T, et al. Runx2 in human breast carcinoma: its potential roles in cancer progression. Cancer Sci. 2010;101:2670-5 pubmed publisher
    b>Runx2 has been proposed as one of the pivotal factors in the process of osteogenesis and metastasis in human malignancies including breast cancer, but its details have not been evaluated...
  74. Niu D, Kondo T, Nakazawa T, Oishi N, Kawasaki T, Mochizuki K, et al. Transcription factor Runx2 is a regulator of epithelial-mesenchymal transition and invasion in thyroid carcinomas. Lab Invest. 2012;92:1181-90 pubmed publisher
    b>Runx2/Cbfa1 is a member of the Runt-related transcription factor family and is an essential regulator of osteoblast/chondrocyte differentiation...
  75. Sancisi V, Borettini G, Maramotti S, Ragazzi M, Tamagnini I, Nicoli D, et al. Runx2 isoform I controls a panel of proinvasive genes driving aggressiveness of papillary thyroid carcinomas. J Clin Endocrinol Metab. 2012;97:E2006-15 pubmed publisher
    ..In a recent work, we reported that the transcriptional regulator Id1 contributes to thyroid cancer progression by powering the invasion capacity of tumor cells...
  76. Brubaker K, Vessella R, Brown L, Corey E. Prostate cancer expression of runt-domain transcription factor Runx2, a key regulator of osteoblast differentiation and function. Prostate. 2003;56:13-22 pubmed
    ..Specific transcription factors, including Runx2, regulate the expression of many bone-related factors in osteoblasts...
  77. Vaughan T, Reid D, Morrison N, Ralston S. RUNX2 alleles associated with BMD in Scottish women; interaction of RUNX2 alleles with menopausal status and body mass index. Bone. 2004;34:1029-36 pubmed
    ..We previously reported the association of the RUNX2 A allele with increased bone mineral density (BMD) and protection against a common form of osteoporotic fracture ..
  78. Doecke J, Day C, Stephens A, Carter S, van Daal A, Kotowicz M, et al. Association of functionally different RUNX2 P2 promoter alleles with BMD. J Bone Miner Res. 2006;21:265-73 pubmed
    b>RUNX2 gene SNPs were genotyped in subjects from the upper and lower deciles of age- and weight-adjusted femoral neck BMD...
  79. Baniwal S, Khalid O, Sir D, Buchanan G, Coetzee G, Frenkel B. Repression of Runx2 by androgen receptor (AR) in osteoblasts and prostate cancer cells: AR binds Runx2 and abrogates its recruitment to DNA. Mol Endocrinol. 2009;23:1203-14 pubmed publisher
    b>Runx2 and androgen receptor (AR) are master transcription factors with pivotal roles in bone metabolism and prostate cancer (PCa)...
  80. Akech J, Wixted J, Bedard K, van der Deen M, Hussain S, Guise T, et al. Runx2 association with progression of prostate cancer in patients: mechanisms mediating bone osteolysis and osteoblastic metastatic lesions. Oncogene. 2010;29:811-21 pubmed publisher
    b>Runx2, a bone-specific transcriptional regulator, is abnormally expressed in highly metastatic prostate cancer cells. Here, we identified the functional activities of Runx2 in facilitating tumor growth and osteolysis...
  81. Pineda B, Hermenegildo C, Laporta P, Tarín J, Cano A, García Pérez M. Common polymorphisms rather than rare genetic variants of the Runx2 gene are associated with femoral neck BMD in Spanish women. J Bone Miner Metab. 2010;28:696-705 pubmed publisher
    b>RUNX2 is a transcription factor essential for osteoblast differentiation and skeletal morphogenesis...
  82. Pierce A, Anglin I, Vitolo M, Mochin M, Underwood K, Goldblum S, et al. Glucose-activated RUNX2 phosphorylation promotes endothelial cell proliferation and an angiogenic phenotype. J Cell Biochem. 2012;113:282-92 pubmed publisher
    The runt-related protein-2 (RUNX2) is a DNA-binding transcription factor that regulates bone formation, tumor cell metastasis, endothelial cell (EC) proliferation, and angiogenesis. RUNX2 DNA binding is glucose and cell cycle regulated...
  83. Underwood K, D Souza D, Mochin Peters M, Pierce A, Kommineni S, Choe M, et al. Regulation of RUNX2 transcription factor-DNA interactions and cell proliferation by vitamin D3 (cholecalciferol) prohormone activity. J Bone Miner Res. 2012;27:913-25 pubmed publisher
    ..b>RUNX2 is a DNA-binding transcription factor that regulates target genes important in bone formation, angiogenesis, and ..
  84. Purcell D, Khalid O, Ou C, Little G, Frenkel B, Baniwal S, et al. Recruitment of coregulator G9a by Runx2 for selective enhancement or suppression of transcription. J Cell Biochem. 2012;113:2406-14 pubmed publisher
    b>Runx2, best known for its role in regulating osteoblast-specific gene expression, also plays an increasingly recognized role in prostate and breast cancer metastasis...
  85. Lee K, Jang W, Kim E, Oh S, Son H, Kim S, et al. Orphan nuclear receptor chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) protein negatively regulates bone morphogenetic protein 2-induced osteoblast differentiation through suppressing runt-related gene 2 (Runx2) activity. J Biol Chem. 2012;287:18888-99 pubmed publisher
    ..Furthermore, the immunoprecipitation assay showed that COUP-TFII and Runx2 physically interacted and COUP-TFII significantly impaired the Runx2-dependent activation of the osteocalcin ..
  86. Dalle Carbonare L, Frigo A, Francia G, Davì M, Donatelli L, Stranieri C, et al. Runx2 mRNA expression in the tissue, serum, and circulating non-hematopoietic cells of patients with thyroid cancer. J Clin Endocrinol Metab. 2012;97:E1249-56 pubmed publisher
    b>Runx2, a master gene of osteogenic differentiation, is also expressed in nonosseous cancer cells. Microcalcifications are characteristic of papillary thyroid carcinoma and represent a useful find for diagnosis...
  87. Ozaki T, Wu D, Sugimoto H, Nagase H, Nakagawara A. Runt-related transcription factor 2 (RUNX2) inhibits p53-dependent apoptosis through the collaboration with HDAC6 in response to DNA damage. Cell Death Dis. 2013;4:e610 pubmed publisher
    Runt-related transcription factor 2 (RUNX2) is the best known as an essential protein for osteoblast differentiation...
  88. Wee H, Huang G, Shigesada K, Ito Y. Serine phosphorylation of RUNX2 with novel potential functions as negative regulatory mechanisms. EMBO Rep. 2002;3:967-74 pubmed
    ..Here, we report a novel observation that suggests that the osteogenesis-associated homologue RUNX2 is negatively regulated by the phosphorylation of two conserved serines (S104 and S451) in two distinct functional ..
  89. Shen R, Wang X, Drissi H, Liu F, O Keefe R, Chen D. Cyclin D1-cdk4 induce runx2 ubiquitination and degradation. J Biol Chem. 2006;281:16347-53 pubmed
    b>Runx2 is a Runt domain transcription factor involved in the activation of genes encoding osteoblast and chondrocyte-specific proteins. Runx2 activity is regulated by transcriptional and post-transcriptional mechanisms...
  90. Lamour V, Detry C, Sanchez C, Henrotin Y, Castronovo V, Bellahcene A. Runx2- and histone deacetylase 3-mediated repression is relieved in differentiating human osteoblast cells to allow high bone sialoprotein expression. J Biol Chem. 2007;282:36240-9 pubmed
    ..human osteosarcoma cells and in differentiating normal human osteoblasts, concomitantly with the decrease of Runx2, a key transcription factor controlling bone formation...
  91. Vladimirova V, Waha A, Lückerath K, Pesheva P, Probstmeier R. Runx2 is expressed in human glioma cells and mediates the expression of galectin-3. J Neurosci Res. 2008;86:2450-61 pubmed publisher
    b>Runx2 is a member of the Runx family of transcription factors (Runx1-3) with a restricted expression pattern...
  92. Komori T. Regulation of bone development and extracellular matrix protein genes by RUNX2. Cell Tissue Res. 2010;339:189-95 pubmed publisher
    b>RUNX2 is a multifunctional transcription factor that controls skeletal development by regulating the differentiation of chondrocytes and osteoblasts and the expression of many extracellular matrix protein genes during chondrocyte and ..