Gene Symbol: RPA2
Description: replication protein A2
Alias: REPA2, RP-A p32, RP-A p34, RPA32, replication protein A 32 kDa subunit, RF-A protein 2, replication factor A protein 2, replication protein A 34 kDa subunit
Species: human
Products:     RPA2

Top Publications

  1. Zimmerman E, Sherman M, Blackett J, Neidleman J, Kreis C, Mundt P, et al. Human immunodeficiency virus type 1 Vpr induces DNA replication stress in vitro and in vivo. J Virol. 2006;80:10407-18 pubmed
    ..We report that infected cells in vivo display an aberrant cell cycle profile whereby a majority of cells have a 4N DNA content, consistent with the onset of G2 arrest. ..
  2. Guan J, Wang H, Perrault A, Wang Y, Iliakis G. Replication protein A2 phosphorylation after DNA damage by the coordinated action of ataxia telangiectasia-mutated and DNA-dependent protein kinase. Cancer Res. 2001;61:8554-63 pubmed
    ..Phosphorylation of the RPA2 subunit is observed after exposure of cells to ionizing radiation (IR) and other DNA-damaging agents, which ..
  3. Dutta A, Stillman B. cdc2 family kinases phosphorylate a human cell DNA replication factor, RPA, and activate DNA replication. EMBO J. 1992;11:2189-99 pubmed
    ..Thus, one or more members of the cyclin-cdc2 kinase family may be required for the initiation and maintenance of S phase, in part due to their ability to phosphorylate and activate a cellular DNA replication factor, RPA. ..
  4. Bastin Shanower S, Brill S. Functional analysis of the four DNA binding domains of replication protein A. The role of RPA2 in ssDNA binding. J Biol Chem. 2001;276:36446-53 pubmed
    ..protein of eukaryotes, contains four ssDNA binding domains (DBDs) within its two largest subunits, RPA1 and RPA2. We analyzed the contribution of the four DBDs to ssDNA binding affinity by assaying recombinant yeast RPA in which ..
  5. Arunkumar A, Klimovich V, Jiang X, Ott R, Mizoue L, Fanning E, et al. Insights into hRPA32 C-terminal domain--mediated assembly of the simian virus 40 replisome. Nat Struct Mol Biol. 2005;12:332-9 pubmed
    ..Corresponding mutations introduced into intact hRPA impaired initiation of replication and primosome activity, implying that this interaction has a critical role in assembly and progression of the SV40 replisome. ..
  6. Niu H, Erdjument Bromage H, Pan Z, Lee S, Tempst P, Hurwitz J. Mapping of amino acid residues in the p34 subunit of human single-stranded DNA-binding protein phosphorylated by DNA-dependent protein kinase and Cdc2 kinase in vitro. J Biol Chem. 1997;272:12634-41 pubmed
    ..We also identified Ser-29 as the major Cdc2 kinase phosphorylation site in the p34 subunit. ..
  7. Kenny M, Schlegel U, Furneaux H, Hurwitz J. The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication. J Biol Chem. 1990;265:7693-700 pubmed
    ..The human SSB 70-kDa subunit appears to be required for DNA unwinding and pol delta stimulation, whereas both the 70- and 34-kDa subunits may be involved in the stimulation of pol alpha. ..
  8. Zernik Kobak M, Vasunia K, Connelly M, Anderson C, Dixon K. Sites of UV-induced phosphorylation of the p34 subunit of replication protein A from HeLa cells. J Biol Chem. 1997;272:23896-904 pubmed
    ..The capacity of purified DNA-PK to phosphorylate many of these same sites on RPA-p34 in vitro implicates DNA-PK or a kinase with similar specificity in the UV-induced hyperphosphorylation of RPA in vivo. ..
  9. Stephan H, Concannon C, Kremmer E, Carty M, Nasheuer H. Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis. Nucleic Acids Res. 2009;37:6028-41 pubmed publisher
    ..protein, replication protein A (RPA), is regulated by the N-terminal phosphorylation of its 32-kDa subunit, RPA2. RPA2 is hyperphosphorylated in response to various DNA-damaging agents and also phosphorylated in a cell-cycle-..

More Information

Publications115 found, 100 shown here

  1. Yusufzai T, Kong X, Yokomori K, Kadonaga J. The annealing helicase HARP is recruited to DNA repair sites via an interaction with RPA. Genes Dev. 2009;23:2400-4 pubmed publisher
    ..These findings suggest that the interaction of HARP with RPA increases the concentration of annealing helicase activity in the vicinity of ssDNA regions to facilitate processes such as DNA repair. ..
  2. Bochkareva E, Korolev S, Lees Miller S, Bochkarev A. Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA. EMBO J. 2002;21:1855-63 pubmed
    ..comprising the C-terminal DNA-binding domain of subunit RPA70 (DBD-C), the central DNA-binding domain of subunit RPA32 (DBD-D) and the entire RPA14 subunit...
  3. DeMott M, Zigman S, Bambara R. Replication protein A stimulates long patch DNA base excision repair. J Biol Chem. 1998;273:27492-8 pubmed
    ..It also has been shown to interact with an early component of base excision repair: uracil glycosylase. Here we show that human replication protein A greatly stimulates long patch base excision repair. ..
  4. Ciccia A, Bredemeyer A, Sowa M, Terret M, Jallepalli P, Harper J, et al. The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart. Genes Dev. 2009;23:2415-25 pubmed publisher
    ..Thus, the symptoms of SIOD may be caused, at least in part, by defects in the cellular response to DNA replication stress...
  5. Lao Y, Gomes X, Ren Y, Taylor J, Wold M. Replication protein A interactions with DNA. III. Molecular basis of recognition of damaged DNA. Biochemistry. 2000;39:850-9 pubmed
    ..These findings suggest that RPA probably binds to both damaged and undamaged strands in the NER excision complex. RPA binding may be important for efficient excision of damaged DNA in NER. ..
  6. Olson E, Nievera C, Klimovich V, Fanning E, Wu X. RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint. J Biol Chem. 2006;281:39517-33 pubmed
    ..We observed that RPA2 is phosphorylated on chromatin in an ATR-dependent manner when replication forks are stalled...
  7. Cai L, Roginskaya M, Qu Y, Yang Z, Xu Y, Zou Y. Structural characterization of human RPA sequential binding to single-stranded DNA using ssDNA as a molecular ruler. Biochemistry. 2007;46:8226-33 pubmed
    Human replication protein A (RPA), a heterotrimer composed of RPA70, RPA32, and RPA14 subunits, contains four single-stranded DNA (ssDNA) binding domains (DBD): DBD-A, DBD-B, and DBD-C in RPA70 and DBD-D in RPA32...
  8. He Z, Henricksen L, Wold M, Ingles C. RPA involvement in the damage-recognition and incision steps of nucleotide excision repair. Nature. 1995;374:566-9 pubmed
    ..In addition, by binding XPG, RPA may target this endonuclease to damaged DNA. ..
  9. Grudic A, Jul Larsen A, Haring S, Wold M, Lønning P, Bjerkvig R, et al. Replication protein A prevents accumulation of single-stranded telomeric DNA in cells that use alternative lengthening of telomeres. Nucleic Acids Res. 2007;35:7267-78 pubmed
    ..This study demonstrates differences between ALT cells and telomerase-positive cells in the requirement for RPA in telomere processing and implicates the ALT mechanism in tumor cells as a possible therapeutic target. ..
  10. Lee D, Pan Y, Kanner S, Sung P, Borowiec J, Chowdhury D. A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination. Nat Struct Mol Biol. 2010;17:365-72 pubmed publisher
    ..Here we show that human protein phosphatase 4 (PP4) dephosphorylates replication protein A (RPA) subunit RPA2, regulating its role in the DSB response...
  11. Shao R, Cao C, Zhang H, Kohn K, Wold M, Pommier Y. Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes. EMBO J. 1999;18:1397-406 pubmed
    ..In human cells treated with the topoisomerase inhibitors camptothecin or etoposide (VP-16), we find that RPA2, the middle-sized subunit of RPA, becomes rapidly phosphorylated...
  12. Weisshart K, Pestryakov P, Smith R, Hartmann H, Kremmer E, Lavrik O, et al. Coordinated regulation of replication protein A activities by its subunits p14 and p32. J Biol Chem. 2004;279:35368-76 pubmed
    ..These data reveal that efficient binding and correct positioning of the RPA complex on single-stranded DNA requires all three subunits to support DNA replication. ..
  13. Din S, Brill S, Fairman M, Stillman B. Cell-cycle-regulated phosphorylation of DNA replication factor A from human and yeast cells. Genes Dev. 1990;4:968-77 pubmed
    ..This observation provides a direct link between a protein required for DNA replication and cell-cycle-regulated protein phosphorylation. ..
  14. Bochkarev A, Bochkareva E, Frappier L, Edwards A. The crystal structure of the complex of replication protein A subunits RPA32 and RPA14 reveals a mechanism for single-stranded DNA binding. EMBO J. 1999;18:4498-504 pubmed
    ..The complex of the two smaller subunits, RPA32 and RPA14, has weak DNA-binding activity but the mechanism of DNA binding is unknown...
  15. Anantha R, Vassin V, Borowiec J. Sequential and synergistic modification of human RPA stimulates chromosomal DNA repair. J Biol Chem. 2007;282:35910-23 pubmed
    ..of human replication protein A (RPA) in DNA replication and repair is regulated by phosphorylation of the middle RPA2 subunit...
  16. Amacker M, Hottiger M, Mossi R, Hubscher U. HIV-1 nucleocapsid protein and replication protein A influence the strand displacement DNA synthesis of lentiviral reverse transcriptase. AIDS. 1997;11:534-6 pubmed
  17. Mer G, Bochkarev A, Gupta R, Bochkareva E, Frappier L, Ingles C, et al. Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA. Cell. 2000;103:449-56 pubmed
    ..We have shown that a globular domain at the C terminus of subunit RPA32 contains a specific surface that interacts in a similar manner with the DNA repair enzyme UNG2 and repair factors ..
  18. Shi B, Xue J, Yin H, Guo R, Luo M, Ye L, et al. Dual functions for the ssDNA-binding protein RPA in meiotic recombination. PLoS Genet. 2019;15:e1007952 pubmed publisher
    ..RPA is a heterotrimer of RPA1, RPA2, and RPA3...
  19. He Y, Yuan J, Qiao Y, Wang D, Chen W, Liu X, et al. The role of carrier ligands of platinum(II) anticancer complexes in the protein recognition of Pt-DNA adducts. Chem Commun (Camb). 2015;51:14064-7 pubmed publisher
    ..Interestingly, an important component of the replication protein A complex, RPA2, was found to bind to kiteplatin much more tightly than other proteins...
  20. Gottschalk A, Timinszky G, Kong S, Jin J, Cai Y, Swanson S, et al. Poly(ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler. Proc Natl Acad Sci U S A. 2009;106:13770-4 pubmed publisher
    ..We propose that poly(ADP-ribosyl)ation of chromatin-associated Parp1 serves as a mechanism for targeting a SNF2 family remodeler to chromatin. ..
  21. Xu Q, Wang F, Xiang Y, Zhang X, Zhao Z, Gao Z, et al. Maternal BCAS2 protects genomic integrity in mouse early embryonic development. Development. 2015;142:3943-53 pubmed publisher
    ..In addition, phosphorylated RPA2 cannot localise to the DNA damage sites in mouse zygotes with disrupted maternal BCAS2...
  22. Byrne B, Oakley G. Replication protein A, the laxative that keeps DNA regular: The importance of RPA phosphorylation in maintaining genome stability. Semin Cell Dev Biol. 2019;86:112-120 pubmed publisher
    ..Phosphorylation of RPA2 is an important post-translational modification of the RPA complex, and is essential for directing context-specific ..
  23. Recolin B, Maiorano D. Implication of RPA32 phosphorylation in S-phase checkpoint signalling at replication forks stalled with aphidicolin in Xenopus egg extracts. Biochem Biophys Res Commun. 2012;427:785-9 pubmed publisher
    ..Here we have investigated the requirement for RPA32 phosphorylation in checkpoint activation in Xenopus egg extracts...
  24. Dong Y, Wang X, Zhang J, Guan Z, Xu L, Wang J, et al. Raltitrexed's effect on the development of neural tube defects in mice is associated with DNA damage, apoptosis, and proliferation. Mol Cell Biochem. 2015;398:223-31 pubmed publisher
    ..DNA damage was determined by detection of phosphorylated replication protein A2 (RPA2) and γ-H2AX in embryos with NTDs induced by RTX...
  25. Zhu J, Su F, Mukherjee S, Mori E, Hu B, Asaithamby A. FANCD2 influences replication fork processes and genome stability in response to clustered DSBs. Cell Cycle. 2015;14:1809-22 pubmed publisher
    ..Mechanistically, FANCD2 is required for correct recruitment of RPA2 and Rad51 to the sites of clustered DSBs and that is critical for proper processing of clustered DSBs...
  26. Mahajan K. hPso4/hPrp19: a critical component of DNA repair and DNA damage checkpoint complexes. Oncogene. 2016;35:2279-86 pubmed publisher
    ..manner, facilitates processing of damaged DNA, promotes DNA end joining, regulates replication protein A (RPA2) phosphorylation and ubiquitination at damaged DNA, but also regulates RNA splicing and mitotic spindle formation ..
  27. Oakley G, Tillison K, Opiyo S, Glanzer J, Horn J, Patrick S. Physical interaction between replication protein A (RPA) and MRN: involvement of RPA2 phosphorylation and the N-terminus of RPA1. Biochemistry. 2009;48:7473-81 pubmed publisher
    Replication protein A (RPA) is a heterotrimeric protein consisting of RPA1, RPA2, and RPA3 subunits that binds to single-stranded DNA (ssDNA) with high affinity...
  28. Ashley A, Shrivastav M, Nie J, Amerin C, Troksa K, Glanzer J, et al. DNA-PK phosphorylation of RPA32 Ser4/Ser8 regulates replication stress checkpoint activation, fork restart, homologous recombination and mitotic catastrophe. DNA Repair (Amst). 2014;21:131-9 pubmed publisher
    ..Activated ATR phosphorylates many targets, including the RPA32 subunit of RPA, leading to Chk1 activation and replication arrest...
  29. Manic G, Signore M, Sistigu A, Russo G, Corradi F, Siteni S, et al. CHK1-targeted therapy to deplete DNA replication-stressed, p53-deficient, hyperdiploid colorectal cancer stem cells. Gut. 2018;67:903-917 pubmed publisher
    ..that CRC-SCs sensitive to LY2606368 displayed signs of ongoing RS response, including the phosphorylation of RPA32 and ataxia telangiectasia mutated serine/threonine kinase (ATM)...
  30. Erdile L, Wold M, Kelly T. The primary structure of the 32-kDa subunit of human replication protein A. J Biol Chem. 1990;265:3177-82 pubmed
    ..The protein made in bacteria is recognized by a monoclonal antibody that is specific for the 32-kDa subunit of RP-A. This monoclonal antibody against the 32-kDa subunit inhibits DNA replication in vitro. ..
  31. Bae S, Bae K, Kim J, Seo Y. RPA governs endonuclease switching during processing of Okazaki fragments in eukaryotes. Nature. 2001;412:456-61 pubmed
    ..We propose a novel biochemical mechanism for the maturation of eukaryotic Okazaki fragments. ..
  32. Xu Y, Wu X, Her C. hMSH5 Facilitates the Repair of Camptothecin-induced Double-strand Breaks through an Interaction with FANCJ. J Biol Chem. 2015;290:18545-58 pubmed publisher
    ..Cells deficient in hMSH5 show elevated CPT-induced γ-H2AX and RPA2 foci with concomitant reduction of Rad51 foci, indicative of impaired homologous recombination...
  33. Hasegawa D, Okabe S, Okamoto K, Nakano I, Shin ya K, Seimiya H. G-quadruplex ligand-induced DNA damage response coupled with telomere dysfunction and replication stress in glioma stem cells. Biochem Biophys Res Commun. 2016;471:75-81 pubmed publisher
    ..and NSGCs had comparable levels of G4s in their nuclei, and both responded to telomestatin with phosphorylation of RPA2 at Ser33-a hallmark of replication stress...
  34. Otterlei M, Warbrick E, Nagelhus T, Haug T, Slupphaug G, Akbari M, et al. Post-replicative base excision repair in replication foci. EMBO J. 1999;18:3834-44 pubmed
    ..These results demonstrate rapid post-replicative removal of incorporated uracil by UNG2 and indicate the formation of a BER complex that contains UNG2, RPA and PCNA close to the replication fork. ..
  35. Yoo E, Kim B, Lee S, Cho C, Chung J, Lee C. 53BP1 is associated with replication protein A and is required for RPA2 hyperphosphorylation following DNA damage. Oncogene. 2005;24:5423-30 pubmed
    ..The 53BP1-associated proteins included RPA1 and RPA2, two components of the replication protein A (RPA) complex...
  36. Sleeth K, Sørensen C, Issaeva N, Dziegielewski J, Bartek J, Helleday T. RPA mediates recombination repair during replication stress and is displaced from DNA by checkpoint signalling in human cells. J Mol Biol. 2007;373:38-47 pubmed
    ..Furthermore, our data suggest that replacement of RPA with the RAD51 and RAD52 proteins is affected by checkpoint signalling. ..
  37. Yan Z, Delannoy M, Ling C, Daee D, Osman F, Muniandy P, et al. A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability. Mol Cell. 2010;37:865-78 pubmed publisher
    ..Thus, FANCM-MHF is an essential DNA-remodeling complex that protects replication forks from yeast to human. ..
  38. Jeong Y, Rossi M, Cermak L, Saraf A, Florens L, Washburn M, et al. FBH1 promotes DNA double-strand breakage and apoptosis in response to DNA replication stress. J Cell Biol. 2013;200:141-9 pubmed publisher
    ..fewer double-strand breaks (DSBs), a dramatic reduction in the activation of ATM and DNA-PK and phosphorylation of RPA2 and p53, and a significantly increased rate of survival. A minor decrease in ssDNA levels was also observed...
  39. Liu T, Huang J. Replication protein A and more: single-stranded DNA-binding proteins in eukaryotic cells. Acta Biochim Biophys Sin (Shanghai). 2016;48:665-70 pubmed publisher
    ..In human, the major SSB, replication protein A (RPA), is a stable heterotrimer composed of subunits of RPA1, RPA2, and RPA3, each of which is conserved not only in mammals but also in all other eukaryotic species...
  40. Zou W, Wang Z, Xiong M, Chen A, Xu P, Ganaie S, et al. Human Parvovirus B19 Utilizes Cellular DNA Replication Machinery for Viral DNA Replication. J Virol. 2018;92: pubmed publisher
    ..We further showed that although RPA32 is essential for B19V DNA replication and the phosphorylated forms of RPA32 colocalized with the replicating viral ..
  41. Wan L, Lou J, Xia Y, Su B, Liu T, Cui J, et al. hPrimpol1/CCDC111 is a human DNA primase-polymerase required for the maintenance of genome integrity. EMBO Rep. 2013;14:1104-12 pubmed publisher
    ..Both RPA1 binding and the primase activity of hPrimpol1 are required for its cellular function during DNA replication. Our results indicate that hPrimpol1 is a novel factor involved in the response to DNA replication stress. ..
  42. Baude A, Aaes T, Zhai B, Al Nakouzi N, Oo H, Daugaard M, et al. Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination. Nucleic Acids Res. 2016;44:2214-26 pubmed publisher
    ..and HeLa cervix carcinoma cells and impairs the DNA damage-induced phosphorylation of replication protein A2 (RPA2) and the recruitment of DNA endonuclease RBBP8/CtIP to DNA double strand breaks...
  43. Nagelhus T, Haug T, Singh K, Keshav K, Skorpen F, Otterlei M, et al. A sequence in the N-terminal region of human uracil-DNA glycosylase with homology to XPA interacts with the C-terminal part of the 34-kDa subunit of replication protein A. J Biol Chem. 1997;272:6561-6 pubmed
    ..the UNG gene (UNG) was found to interact with the C-terminal part of the 34-kDa subunit of replication protein A (RPA2). No interaction with RPA4 (a homolog of RPA2), RPA1, or RPA3 was observed...
  44. Nomaguchi M, Adachi A. HIV-1 Vpr and G2 cell cycle arrest. Future Microbiol. 2011;6:375-8 pubmed publisher
    ..They demonstrate, mainly by confocal immunofluorescence analysis, that highly mobile chromatin-associated Vpr nuclear foci are critical for induction of the G2 cell cycle arrest. ..
  45. Brocardo M, Borowiec J, Henderson B. Adenomatous polyposis coli protein regulates the cellular response to DNA replication stress. Int J Biochem Cell Biol. 2011;43:1354-64 pubmed publisher
    ..Immunoprecipitation assays revealed a selective binding of APC to replication protein A 32kDa subunit (RPA32), and the APC-RPA32 complex increased at chromatin after hydroxyurea treatment...
  46. Wang J, Yang T, Chen H, Li H, Zheng S. Oncogene RPA1 promotes proliferation of hepatocellular carcinoma via CDK4/Cyclin-D pathway. Biochem Biophys Res Commun. 2018;498:424-430 pubmed publisher
    ..plays a central role in eukaryotic DNA replication, homologous recombination, and excision repair, including RPA1, RPA2 and RPA3...
  47. Habel J, Ohren J, Borgstahl G. Dynamic light-scattering analysis of full-length human RPA14/32 dimer: purification, crystallization and self-association. Acta Crystallogr D Biol Crystallogr. 2001;57:254-9 pubmed
    ..0, c = 272.6 A) and to 2.2 and 1.9 A resolution from two orthorhombic crystal forms (both P2(1)2(1)2(1); form I, a = 61.4, b = 75.2, c = 131.6 A; form II, a = 81.8, b = 140.4, c = 173.1 A). ..
  48. Ahn J, Kim S, Na W, Baek S, Kim J, Min K, et al. SERBP1 affects homologous recombination-mediated DNA repair by regulation of CtIP translation during S phase. Nucleic Acids Res. 2015;43:6321-33 pubmed publisher
    ..In response to camptothecin-mediated DNA DSBs, CHK1 and RPA2 phosphorylation, which are hallmarks of HR activation, was abrogated in SERBP1-depleted cells...
  49. Deng X, Xu P, Zou W, Shen W, Peng J, Liu K, et al. DNA Damage Signaling Is Required for Replication of Human Bocavirus 1 DNA in Dividing HEK293 Cells. J Virol. 2017;91: pubmed publisher
    ..HEK293 cells with the duplex DNA genome of HBoV1 induces hallmarks of DDR, including phosphorylation of H2AX and RPA32, as well as activation of all three PI3KKs...
  50. Dubois J, Yates M, Gaudreau Lapierre A, Clement G, Cappadocia L, Gaudreau L, et al. A phosphorylation-and-ubiquitylation circuitry driving ATR activation and homologous recombination. Nucleic Acids Res. 2017;45:8859-8872 pubmed publisher
    ..An RPA32 mutant lacking phosphorylation sites fails to recruit PRP19 and support RPA ubiquitylation...
  51. Tian Y, Liu G, Wang H, Tian Z, Cai Z, Zhang F, et al. Valproic acid sensitizes breast cancer cells to hydroxyurea through inhibiting RPA2 hyperphosphorylation-mediated DNA repair pathway. DNA Repair (Amst). 2017;58:1-12 pubmed publisher
    ..through inhibiting the activity of two key HR repair proteins by hyperphosphorylation of replication protein A2 (RPA2-p) and recombinase Rad51. However, apoptosis was not detected under this condition...
  52. Liu S, Chu J, Yucer N, Leng M, Wang S, Chen B, et al. RING finger and WD repeat domain 3 (RFWD3) associates with replication protein A (RPA) and facilitates RPA-mediated DNA damage response. J Biol Chem. 2011;286:22314-22 pubmed publisher
    ..These findings suggest that RFWD3 is recruited to sites of DNA damage and facilitates RPA-mediated DNA damage signaling and repair. ..
  53. Hu J, Hu Z, Zhang Y, Gou X, Mu Y, Wang L, et al. Metal binding mediated conformational change of XPA protein:a potential cytotoxic mechanism of nickel in the nucleotide excision repair. J Mol Model. 2016;22:156 pubmed publisher
    ..e., RPA70N, RPA70A, RPA70B, RPA70C, RPA32, and RPA14) based on previously reported structural biology information...
  54. Kang T, Lindsey Boltz L, Reardon J, Sancar A. Circadian control of XPA and excision repair of cisplatin-DNA damage by cryptochrome and HERC2 ubiquitin ligase. Proc Natl Acad Sci U S A. 2010;107:4890-5 pubmed publisher
    ..These findings may be used as a guide for timing of cisplatin chemotherapy. ..
  55. Belzile J, Abrahamyan L, Gerard F, Rougeau N, Cohen E. Formation of mobile chromatin-associated nuclear foci containing HIV-1 Vpr and VPRBP is critical for the induction of G2 cell cycle arrest. PLoS Pathog. 2010;6:e1001080 pubmed publisher
    ..nuclear foci contain VPRBP and partially overlap with DNA repair foci components such as gamma-H2AX, 53BP1 and RPA32. While treatment with the non-specific ATR inhibitor caffeine or depletion of VPRBP by siRNA did not inhibit ..
  56. Kubasova T, Matiasovicova J, Rychlik I, Juricova H. Complete sequence of multidrug resistance p9134 plasmid and its variants including natural recombinant with the virulence plasmid of Salmonella serovar Typhimurium. Plasmid. 2014;76:8-14 pubmed publisher
    ..During the second one, ydjA, pifA and repC genes from p9134 were replaced with repA2, PSLT025 and PSLT026 genes from pSLT...
  57. Zuazua Villar P, Ganesh A, Phear G, Gagou M, Meuth M. Extensive RPA2 hyperphosphorylation promotes apoptosis in response to DNA replication stress in CHK1 inhibited cells. Nucleic Acids Res. 2015;43:9776-87 pubmed publisher
    ..When CHK1 is inhibited during DNA replication stress, RPA2 is extensively hyperphosphorylated...
  58. Kudoh A, Iwahori S, Sato Y, Nakayama S, Isomura H, Murata T, et al. Homologous recombinational repair factors are recruited and loaded onto the viral DNA genome in Epstein-Barr virus replication compartments. J Virol. 2009;83:6641-51 pubmed publisher
    ..The hyperphosphorylation of RPA32 causes a change in RPA conformation, resulting in a switch from the catalysis of DNA replication to the ..
  59. Fan W, Luo J. SIRT1 regulates UV-induced DNA repair through deacetylating XPA. Mol Cell. 2010;39:247-58 pubmed publisher
    ..Furthermore, SIRT1-mediated XPA deacetylation enhances its interaction with RPA32. Our results demonstrate that SIRT1 regulates NER pathway through modulation of XPA acetylation status.
  60. Hérate C, Vigne C, Guenzel C, Lambelé M, Rouyez M, Benichou S. Uracil DNA glycosylase interacts with the p32 subunit of the replication protein A complex to modulate HIV-1 reverse transcription for optimal virus dissemination. Retrovirology. 2016;13:26 pubmed publisher
    ..We report here that Vpr can form a trimolecular complex with UNG2 and the p32 subunit (RPA32) of the replication protein A (RPA) complex and we explore how these cellular proteins can influence virus ..
  61. McIlwraith M, Van Dyck E, Masson J, Stasiak A, Stasiak A, West S. Reconstitution of the strand invasion step of double-strand break repair using human Rad51 Rad52 and RPA proteins. J Mol Biol. 2000;304:151-64 pubmed
  62. Chen C, Lee J, Chang M. 4E-BP3 regulates eIF4E-mediated nuclear mRNA export and interacts with replication protein A2. FEBS Lett. 2012;586:2260-6 pubmed publisher
    ..Furthermore, 4E-BP3 interacted with dephosphorylated RPA2 (replication protein A2)...
  63. Costello J, Frühwald M, Smiraglia D, Rush L, Robertson G, Gao X, et al. Aberrant CpG-island methylation has non-random and tumour-type-specific patterns. Nat Genet. 2000;24:132-8 pubmed
    ..The expression of many of these genes was reactivated by experimental demethylation in cultured tumour cells. Thus, the methylation of particular subsets of CpG islands may have consequences for specific tumour types. ..
  64. Chan D, Son S, Block W, Ye R, Khanna K, Wold M, et al. Purification and characterization of ATM from human placenta. A manganese-dependent, wortmannin-sensitive serine/threonine protein kinase. J Biol Chem. 2000;275:7803-10 pubmed
    ..The related protein, DNA-dependent protein kinase catalytic subunit, also phosphorylated PHAS-I, RPA, and Chk2 in the presence of manganese, suggesting that the requirement for manganese is a characteristic of this class of enzyme. ..
  65. Chen C, Juan C, Chen K, Chang Y, Lee J, Chang M. Upregulation of RPA2 promotes NF-κB activation in breast cancer by relieving the antagonistic function of menin on NF-κB-regulated transcription. Carcinogenesis. 2017;38:196-206 pubmed publisher
    b>RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. In this study, we showed a significant RPA2 upregulation in breast cancer tissues and cell lines...
  66. Wong J, Ionescu D, Ingles C. Interaction between BRCA2 and replication protein A is compromised by a cancer-predisposing mutation in BRCA2. Oncogene. 2003;22:28-33 pubmed
    ..Therefore, the transcriptional activation domains within BRCA2, and perhaps BRCA1, may provide links to RPA and DNA repair processes rather than transcription. ..
  67. Niu H, Chung W, Zhu Z, Kwon Y, Zhao W, Chi P, et al. Mechanism of the ATP-dependent DNA end-resection machinery from Saccharomyces cerevisiae. Nature. 2010;467:108-11 pubmed publisher
    ..Our reconstituted system should serve as a useful model for delineating the mechanistic intricacy of the DNA break resection process in eukaryotes. ..
  68. Harley M, Murina O, Leitch A, Higgs M, Bicknell L, Yigit G, et al. TRAIP promotes DNA damage response during genome replication and is mutated in primordial dwarfism. Nat Genet. 2016;48:36-43 pubmed publisher
    ..that TRAIP relocalizes to sites of DNA damage, where it is required for optimal phosphorylation of H2AX and RPA2 during S-phase in response to ultraviolet (UV) irradiation, as well as fork progression through UV-induced DNA ..
  69. Wood R, Shivji M. Which DNA polymerases are used for DNA-repair in eukaryotes?. Carcinogenesis. 1997;18:605-10 pubmed
    ..A more recently discovered enzyme, DNA polymerase zeta, appears to be involved in the bypass of damage, without excision, and occurs during DNA replication of a damaged template. ..
  70. Levidou G, Gakiopoulou H, Kavantzas N, Saetta A, Karlou M, Pavlopoulos P, et al. Prognostic significance of replication protein A (RPA) expression levels in bladder urothelial carcinoma. BJU Int. 2011;108:E59-65 pubmed publisher
    ..Paraffin-embedded tissue from 156 patients with bladder UC was immunostained for RPA1 and RPA2. RPA1 and RPA2 labelling indexes (LIs) decreased with increasing histological grade (both P < 0...
  71. Broderick R, Rainey M, Santocanale C, Nasheuer H. Cell cycle-dependent formation of Cdc45-Claspin complexes in human cells is compromized by UV-mediated DNA damage. FEBS J. 2013;280:4888-902 pubmed publisher
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    ..13 finally selected for the age tool based on MPS (DDO, ELOVL2, F5, GRM2, HOXC4, KLF14, LDB2, MEIS1-AS3, NKIRAS2, RPA2, SAMD10, TRIM59, ZYG11A)...
  73. Lin Y, Shivji M, Chen C, Kolodner R, Wood R, Dutta A. The evolutionarily conserved zinc finger motif in the largest subunit of human replication protein A is required for DNA replication and mismatch repair but not for nucleotide excision repair. J Biol Chem. 1998;273:1453-61 pubmed
    ..Therefore, the zinc finger of the largest subunit of RPA is required for a function that is essential for DNA replication and mismatch repair but not for nucleotide excision repair. ..
  74. Feldkamp M, Mason A, Eichman B, Chazin W. Structural analysis of replication protein A recruitment of the DNA damage response protein SMARCAL1. Biochemistry. 2014;53:3052-61 pubmed publisher
    ..The implications of our results are discussed with respect to the recruitment of SMARCAL1 and other DNA damage response and repair proteins to stalled replication forks. ..
  75. Treuner K, Findeisen M, Strausfeld U, Knippers R. Phosphorylation of replication protein A middle subunit (RPA32) leads to a disassembly of the RPA heterotrimer. J Biol Chem. 1999;274:15556-61 pubmed
    ..A (RPA), the major eukaryotic single-strand specific DNA binding protein, consists of three subunits, RPA70, RPA32, and RPA14. The middle subunit, RPA32, is phosphorylated in a cell cycle-dependent manner...
  76. Safa L, Delagoutte E, Petruseva I, Alberti P, Lavrik O, Riou J, et al. Binding polarity of RPA to telomeric sequences and influence of G-quadruplex stability. Biochimie. 2014;103:80-8 pubmed publisher
    ..Our results showed that RPA1 and RPA2 are arranged from 5' to 3' along the unfolded telomeric G4, as already described for unstructured single-stranded ..
  77. Araújo S, Nigg E, Wood R. Strong functional interactions of TFIIH with XPC and XPG in human DNA nucleotide excision repair, without a preassembled repairosome. Mol Cell Biol. 2001;21:2281-91 pubmed
    ..A panel of antibodies was used to estimate that there are on the order of 10(5) molecules of each core NER factor per HeLa cell. ..
  78. Abu Zhayia E, Awwad S, Ben Oz B, Khoury Haddad H, Ayoub N. CDYL1 fosters double-strand break-induced transcription silencing and promotes homology-directed repair. J Mol Cell Biol. 2017;: pubmed publisher
    ..DNA damage induction, CDYL1 depletion causes persistent G2/M arrest and alters H2AX and replication protein A (RPA2) phosphorylation...
  79. Golub E, Gupta R, Haaf T, Wold M, Radding C. Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA. Nucleic Acids Res. 1998;26:5388-93 pubmed
    ..The interactions of human RPA with Rad51, replication proteins and DNA are suited to the linking of recombination to replication. ..
  80. Ahel D, Horejsí Z, Wiechens N, Polo S, Garcia Wilson E, Ahel I, et al. Poly(ADP-ribose)-dependent regulation of DNA repair by the chromatin remodeling enzyme ALC1. Science. 2009;325:1240-3 pubmed publisher
    ..Furthermore, we show that depletion or overexpression of ALC1 results in sensitivity to DNA-damaging agents. Collectively, these results provide new insights into the mechanisms by which poly(ADP-ribose) regulates DNA repair. ..
  81. Shi W, Feng Z, Zhang J, Gonzalez Suarez I, VanderWaal R, Wu X, et al. The role of RPA2 phosphorylation in homologous recombination in response to replication arrest. Carcinogenesis. 2010;31:994-1002 pubmed publisher
    ..However, the role of RPA2 hyperphosphorylation, which occurs in response to DNA damage, had been unclear...
  82. Liaw H, Lee D, Myung K. DNA-PK-dependent RPA2 hyperphosphorylation facilitates DNA repair and suppresses sister chromatid exchange. PLoS ONE. 2011;6:e21424 pubmed publisher
    Hyperphosphorylation of RPA2 at serine 4 and serine 8 (S4, S8) has been used as a marker for activation of the DNA damage response...
  83. Biard D, Miccoli L, Despras E, Harper F, Pichard E, Creminon C, et al. Participation of kin17 protein in replication factories and in other DNA transactions mediated by high molecular weight nuclear complexes. Mol Cancer Res. 2003;1:519-31 pubmed
    ..analyses of cellular extracts showed that endogenous (HSA)kin17 protein co-eluted with both replication proteins RPA32 and RPA70 in a fraction containing complexes of M(r) 600,000...
  84. Bouley J, Pionneau C, Varinot J, Biard D, Genestie C, Antoine M, et al. Proteomic analysis of BRCA1-depleted cell line reveals a putative role for replication protein A2 up-regulation in BRCA1 breast tumor development. Proteomics Clin Appl. 2010;4:489-98 pubmed publisher
    ..We detected increased levels of Replication protein A2 (RPA2) in BRCA1(KD) cells as compared to control cells...
  85. Guenzel C, Hérate C, Le Rouzic E, Maidou Peindara P, Sadler H, Rouyez M, et al. Recruitment of the nuclear form of uracil DNA glycosylase into virus particles participates in the full infectivity of HIV-1. J Virol. 2012;86:2533-44 pubmed publisher
  86. Salas T, Petruseva I, Lavrik O, Saintome C. Evidence for direct contact between the RPA3 subunit of the human replication protein A and single-stranded DNA. Nucleic Acids Res. 2009;37:38-46 pubmed publisher
    ..It is a heterotrimeric complex consisting of three subunits: RPA1, RPA2 and RPA3...
  87. Friboulet L, Postel Vinay S, Sourisseau T, Adam J, Stoclin A, Ponsonnailles F, et al. ERCC1 function in nuclear excision and interstrand crosslink repair pathways is mediated exclusively by the ERCC1-202 isoform. Cell Cycle. 2013;12:3298-306 pubmed publisher
    ..These data suggest that the selective assessment of the ERCC1 isoform 202 in clinical samples should accurately reflect the DDR-related activity of the gene and hence constitute a useful biomarker for customizing anticancer therapies. ..
  88. Brush G, Kelly T. Phosphorylation of the replication protein A large subunit in the Saccharomyces cerevisiae checkpoint response. Nucleic Acids Res. 2000;28:3725-32 pubmed
  89. Liu Y, Vaithiyalingam S, Shi Q, Chazin W, Zinkel S. BID binds to replication protein A and stimulates ATR function following replicative stress. Mol Cell Biol. 2011;31:4298-309 pubmed publisher
    ..We propose a model in which BID associates with RPA and stimulates the recruitment and/or stabilization of ATR-ATRIP to the DNA damage sensor complex. ..
  90. Vaithiyalingam S, Warren E, Eichman B, Chazin W. Insights into eukaryotic DNA priming from the structure and functional interactions of the 4Fe-4S cluster domain of human DNA primase. Proc Natl Acad Sci U S A. 2010;107:13684-9 pubmed publisher
    ..Together, our results explain functional defects in human DNA primase mutants and provide insights into primosome loading on RPA-coated ssDNA and regulation of primase activity. ..
  91. Xie S, Lu Y, Jakoncic J, Sun H, Xia J, Qian C. Structure of RPA32 bound to the N-terminus of SMARCAL1 redefines the binding interface between RPA32 and its interacting proteins. FEBS J. 2014;281:3382-96 pubmed publisher
    Replication protein A subunit RPA32 contains a C-terminal domain that interacts with a variety of DNA damage response proteins including SMARCAL1, Tipin, UNG2 and XPA...
  92. Zhang H, Gan H, Wang Z, Lee J, Zhou H, ORDOG T, et al. RPA Interacts with HIRA and Regulates H3.3 Deposition at Gene Regulatory Elements in Mammalian Cells. Mol Cell. 2017;65:272-284 pubmed publisher
    ..3 to these regulatory elements for gene regulation. ..