Gene Symbol: RNPS1
Description: RNA binding protein with serine rich domain 1
Alias: E5.1, RNA-binding protein with serine-rich domain 1, RNA binding protein with serine-rich domain, RNA-binding protein S1, serine-rich domain, SR protein, SR-related protein LDC2
Species: human
Products:     RNPS1

Top Publications

  1. Le Hir H, Izaurralde E, Maquat L, Moore M. The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions. EMBO J. 2000;19:6860-9 pubmed
    ..revealed that five components of the complex are the splicing-associated factors SRm160, DEK and RNPS1, the mRNA-associated shuttling protein Y14 and the mRNA export factor REF...
  2. Sakashita E, Tatsumi S, Werner D, Endo H, Mayeda A. Human RNPS1 and its associated factors: a versatile alternative pre-mRNA splicing regulator in vivo. Mol Cell Biol. 2004;24:1174-87 pubmed
    ..Four factors were identified: p54 (also called SRp54; a member of the SR protein family), human transformer 2 beta (hTra2 beta; an exonic splicing enhancer-binding protein), hLucA (a potential ..
  3. McCracken S, Longman D, Johnstone I, Caceres J, Blencowe B. An evolutionarily conserved role for SRm160 in 3'-end processing that functions independently of exon junction complex formation. J Biol Chem. 2003;278:44153-60 pubmed
    ..The EJC components RNPS1, REF, UAP56, and Y14 interact with SRm160...
  4. Li C, Lin R, Lai M, Ouyang P, Tarn W. Nuclear Pnn/DRS protein binds to spliced mRNPs and participates in mRNA processing and export via interaction with RNPS1. Mol Cell Biol. 2003;23:7363-76 pubmed
    ..The potential interaction of Pnn with RNPS1, a pre-mRNA splicing factor and a component of the exon-exon junction complex, prompted us to examine whether Pnn ..
  5. Gonatopoulos Pournatzis T, Wu M, Braunschweig U, Roth J, Han H, Best A, et al. Genome-wide CRISPR-Cas9 Interrogation of Splicing Networks Reveals a Mechanism for Recognition of Autism-Misregulated Neuronal Microexons. Mol Cell. 2018;72:510-524.e12 pubmed publisher
    ..Remarkably, the widely expressed RNA binding proteins Srsf11 and Rnps1 directly, preferentially, and frequently co-activate these microexons...
  6. Boehm V, Britto Borges T, Steckelberg A, Singh K, Gerbracht J, Gueney E, et al. Exon Junction Complexes Suppress Spurious Splice Sites to Safeguard Transcriptome Integrity. Mol Cell. 2018;72:482-495.e7 pubmed publisher
    ..Mechanistically, the EJC-mediated recruitment of the splicing regulator RNPS1 inhibits cryptic 5'SS usage, while the deposition of the EJC core directly masks reconstituted 3'SS, thereby ..
  7. Mabin J, Woodward L, Patton R, Yi Z, Jia M, Wysocki V, et al. The Exon Junction Complex Undergoes a Compositional Switch that Alters mRNP Structure and Nonsense-Mediated mRNA Decay Activity. Cell Rep. 2018;25:2431-2446.e7 pubmed publisher
    ..To illuminate consequences of EJC composition change, we purified EJCs from human cells via peripheral proteins RNPS1 and CASC3...
  8. Blázquez L, Emmett W, Faraway R, Pineda J, Bajew S, Gohr A, et al. Exon Junction Complex Shapes the Transcriptome by Repressing Recursive Splicing. Mol Cell. 2018;72:496-509.e9 pubmed publisher
    ..The core EJC factors, and the peripheral factors PNN and RNPS1, maintain RS-exon inclusion by repressing spliceosomal assembly on RS-5ss...
  9. Wang F, Soprano K, Soprano D. Role of Acinus in regulating retinoic acid-responsive gene pre-mRNA splicing. J Cell Physiol. 2015;230:791-801 pubmed publisher
    ..the RA-dependent splicing activity of Acinus and the RA-independent splicing activity of Acinus is repressed by RNPS1. Importantly, measurement of the splicing of endogenous human RARβ and Bcl-x in vivo demonstrates that Acinus ..

More Information


  1. Fukumura K, Inoue K, Mayeda A. Splicing activator RNPS1 suppresses errors in pre-mRNA splicing: A key factor for mRNA quality control. Biochem Biophys Res Commun. 2018;496:921-926 pubmed publisher
    Human RNPS1 protein was first identified as a pre-mRNA splicing activator in vitro and RNPS1 regulates alternative splicing in cellulo. RNPS1 was also known as a peripheral factor of the exon junction complex (EJC)...
  2. Marayati B, Hoskins V, Boger R, Tucker J, Fishman E, Bray A, et al. The fission yeast MTREC and EJC orthologs ensure the maturation of meiotic transcripts during meiosis. RNA. 2016;22:1349-59 pubmed publisher
    ..initiation factor eIF4aIII in the fission yeast Schizosaccharomyces pombe In mammals, together with MAGO (Mnh1), Rnps1, and Y14, elF4AIII (pFal1) forms the core of the exon junction complex (EJC), which is essential for ..
  3. Hauer C, Sieber J, Schwarzl T, Hollerer I, Curk T, Alleaume A, et al. Exon Junction Complexes Show a Distributional Bias toward Alternatively Spliced mRNAs and against mRNAs Coding for Ribosomal Proteins. Cell Rep. 2016;16:1588-1603 pubmed publisher
    ..EJC binding sites across the transcriptome including all four RNA binding EJC components eIF4A3, BTZ, UPF3B, and RNPS1. Integration of these data sets permits definition of high-confidence EJC deposition sites as well as assessment ..
  4. Xing D, Wang Y, Hamilton M, Ben Hur A, Reddy A. Transcriptome-Wide Identification of RNA Targets of Arabidopsis SERINE/ARGININE-RICH45 Uncovers the Unexpected Roles of This RNA Binding Protein in RNA Processing. Plant Cell. 2015;27:3294-308 pubmed publisher
    Plant SR45 and its metazoan ortholog RNPS1 are serine/arginine-rich (SR)-like RNA binding proteins that function in splicing/postsplicing events and regulate diverse processes in eukaryotes...
  5. Kwon S, Kim E, Baek K. RNPS1 is modulated by ubiquitin-specific protease 4. FEBS Lett. 2017;591:369-381 pubmed publisher
    RNA-binding protein with serine-rich domain 1 (RNPS1) is a component of pre-splicing and post-splicing multiprotein complexes, which activates constitutive and alternative splicing...
  6. Zhang X, Shi Y, Powers J, Gowda N, Zhang C, Ibrahim H, et al. Transcriptome analyses reveal SR45 to be a neutral splicing regulator and a suppressor of innate immunity in Arabidopsis thaliana. BMC Genomics. 2017;18:772 pubmed publisher
    ..Although SR45 has orthologs in diverse eukaryotes, including human RNPS1, the sr45-1 null mutant is viable...
  7. Kataoka N, Diem M, Kim V, Yong J, Dreyfuss G. Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex. EMBO J. 2001;20:6424-33 pubmed
    ..We show that Y14 binds to two previously reported components, Aly/REF and RNPS1, and to the mRNA export factor TAP...
  8. Lykke Andersen J, Shu M, Steitz J. Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1. Science. 2001;293:1836-9 pubmed
    ..Here, we demonstrate that the protein RNPS1, a component of the postsplicing complex that is deposited 5' to exon-exon junctions, interacts with the ..
  9. An M, Kim C, Nam G, Kim D, Rhee S, Cho S, et al. Transcriptome analysis for UVB-induced phototoxicity in mouse retina. Environ Toxicol. 2018;33:52-62 pubmed publisher
    ..factors (eg, Asxl2, Atf7, Per1, Phox2a, Rxra), RNA processing, and neuronal genes (eg, B4gal2, Drd1, Grm5, Rnf40, Rnps1, Usp39, Wbp4). The differentially expressed genes from the RNA-seq analysis were validated by quantitative PCR...
  10. Polprasert C, Schulze I, Sekeres M, Makishima H, Przychodzen B, Hosono N, et al. Inherited and Somatic Defects in DDX41 in Myeloid Neoplasms. Cancer Cell. 2015;27:658-70 pubmed publisher
    ..DDX41 lesions caused altered pre-mRNA splicing and RNA processing. DDX41 is exemplary of other RNA helicase genes also affected by somatic mutations, suggesting that they constitute a family of tumor suppressor genes. ..
  11. Zhang X, Mount S. Two alternatively spliced isoforms of the Arabidopsis SR45 protein have distinct roles during normal plant development. Plant Physiol. 2009;150:1450-8 pubmed publisher
    ..1-T218A + S219A-GFP) behaves like isoform 2, complementing the root but not the floral phenotype. In conclusion, our study provides evidence that the two alternatively spliced isoforms of SR45 have distinct biological functions...
  12. Li X, Niu T, Manley J. The RNA binding protein RNPS1 alleviates ASF/SF2 depletion-induced genomic instability. RNA. 2007;13:2108-15 pubmed
    ..We have recently shown that the SR protein ASF/SF2 prevents R-loop formation in vertebrates by cotranscriptionally binding to nascent mRNA precursors to ..
  13. Badolato J, Gardiner E, Morrison N, Eisman J. Identification and characterisation of a novel human RNA-binding protein. Gene. 1995;166:323-7 pubmed
    ..E5.1 may, therefore, represent a novel gene, encoding a protein involved in processing of precursor RNAs into mature mRNA. ..
  14. Harada K, Yamada A, Yang D, Itoh K, Shichijo S. Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation. Int J Cancer. 2001;93:623-8 pubmed
    ..factor (RNA-binding protein prevalent during the S phase or RNA-binding protein with a serine-rich domain [RNPS1]) that activated both constitutive and alternative splicing of pre-mRNA in vitro...
  15. Kimura T, Hashimoto I, Nishizawa M, Ito S, Yamada H. Novel cis-active structures in the coding region mediate CRM1-dependent nuclear export of IFN-? 1 mRNA. Med Mol Morphol. 2010;43:145-57 pubmed publisher
    ..An RNPS1 (RNA-binding protein S1, serine-rich domain) cDNA was isolated by yeast three-hybrid screening, using bait ..
  16. Trembley J, Tatsumi S, Sakashita E, Loyer P, Slaughter C, Suzuki H, et al. Activation of pre-mRNA splicing by human RNPS1 is regulated by CK2 phosphorylation. Mol Cell Biol. 2005;25:1446-57 pubmed
    Human RNPS1 was originally characterized as a pre-mRNA splicing activator in vitro and was shown to regulate alternative splicing in vivo...
  17. Murachelli A, Ebert J, Basquin C, Le Hir H, Conti E. The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex. Nat Struct Mol Biol. 2012;19:378-86 pubmed publisher
    ..The three ASAP subunits (Acinus, RNPS1 and SAP18) have been individually implicated in transcriptional regulation, pre-mRNA splicing and mRNA quality ..
  18. Wilson K, Fortes P, Singh U, Ohno M, Mattaj I, Cerione R. The nuclear cap-binding complex is a novel target of growth factor receptor-coupled signal transduction. J Biol Chem. 1999;274:4166-73 pubmed
    ..Taken together, these data identify the CBC as a nuclear target for growth factor-coupled signal transduction and suggest novel mechanisms by which growth factors can influence gene expression and cell growth. ..
  19. Kataoka N, Dreyfuss G. A simple whole cell lysate system for in vitro splicing reveals a stepwise assembly of the exon-exon junction complex. J Biol Chem. 2004;279:7009-13 pubmed
    ..Three of the EJC components, Aly/REF, RNPS1, and SRm160, are found on pre-mRNA by the time the spliceosome is formed, whereas Upf3b associates with splicing ..
  20. Lejeune F, Ishigaki Y, Li X, Maquat L. The exon junction complex is detected on CBP80-bound but not eIF4E-bound mRNA in mammalian cells: dynamics of mRNP remodeling. EMBO J. 2002;21:3536-45 pubmed
    ..Consistent with this evidence, we demonstrate that RNPS1, Y14, SRm160, REF/Aly, TAP, Upf3X and Upf2 are detected in the nuclear fraction on CBP80-bound but not eIF4E-bound ..
  21. Mabuchi F, Sakurada Y, Kashiwagi K, Yamagata Z, Iijima H, Tsukahara S. Association between SRBD1 and ELOVL5 gene polymorphisms and primary open-angle glaucoma. Invest Ophthalmol Vis Sci. 2011;52:4626-9 pubmed publisher
    ..SRBD1 gene polymorphism is associated with the development of HTG as well as NTG, including late-onset NTG. Typical POAG associated with ELOVL5 gene polymorphism may have a late rather than an early onset. ..
  22. Burn T, Connors T, Van Raay T, Dackowski W, Millholland J, Klinger K, et al. Generation of a transcriptional map for a 700-kb region surrounding the polycystic kidney disease type 1 (PKD1) and tuberous sclerosis type 2 (TSC2) disease genes on human chromosome 16p3.3. Genome Res. 1996;6:525-37 pubmed
    ..In this report we present a transcriptional map for the 700 kb of DNA surrounding the PKD1 and TSC2 genes. ..
  23. Joselin A, Schulze Osthoff K, Schwerk C. Loss of Acinus inhibits oligonucleosomal DNA fragmentation but not chromatin condensation during apoptosis. J Biol Chem. 2006;281:12475-84 pubmed
    ..We show that Acinus is not required for nuclear localization and interaction of the other ASAP subunits SAP18 and RNPS1; however, knockdown of Acinus leads to a reduction in cell growth...
  24. Lee K, Hsu I, Tarn W. TRAP150 activates pre-mRNA splicing and promotes nuclear mRNA degradation. Nucleic Acids Res. 2010;38:3340-50 pubmed publisher
    ..Moreover, TRAP150 activates pre-mRNA splicing and induces mRNA degradation by its separable functional domains. Therefore, TRAP150 represents a multi-functional protein involved in nuclear mRNA metabolism. ..
  25. Loyer P, Trembley J, Lahti J, Kidd V. The RNP protein, RNPS1, associates with specific isoforms of the p34cdc2-related PITSLRE protein kinase in vivo. J Cell Sci. 1998;111 ( Pt 11):1495-506 pubmed
    ..nuclear speckles, and that these isoforms specifically interact in vitro and in vivo with the RNA-binding protein RNPS1. RNPS1 is also localized to nuclear speckles, and its over expression disrupts normal nuclear speckle organization ..
  26. Schwerk C, Prasad J, Degenhardt K, Erdjument Bromage H, White E, Tempst P, et al. ASAP, a novel protein complex involved in RNA processing and apoptosis. Mol Cell Biol. 2003;23:2981-90 pubmed
    ..ASAP complexes are composed of the polypeptides SAP18 and RNPS1 and different isoforms of the Acinus protein...
  27. Mayeda A, Badolato J, Kobayashi R, Zhang M, Gardiner E, Krainer A. Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing. EMBO J. 1999;18:4560-70 pubmed
    ..distal alternative 3' splice sites in a concentration-dependent manner resulted in the identification of RNPS1, a novel general activator of pre-mRNA splicing...
  28. Michelle L, Cloutier A, Toutant J, Shkreta L, Thibault P, Durand M, et al. Proteins associated with the exon junction complex also control the alternative splicing of apoptotic regulators. Mol Cell Biol. 2012;32:954-67 pubmed publisher
    ..Depleting proteins known as core (Y14 and eIF4A3) or auxiliary (RNPS1, Acinus, and SAP18) components of the exon junction complex (EJC) improved the production of the proapoptotic Bcl-..
  29. Kim V, Kataoka N, Dreyfuss G. Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex. Science. 2001;293:1832-6 pubmed
    ..The splicing-dependent binding of hUpf3 to mRNAs before export, as part of the complex that assembles near exon-exon junctions, allows it to serve as a link between splicing and NMD in the cytoplasm. ..
  30. Nott A, Le Hir H, Moore M. Splicing enhances translation in mammalian cells: an additional function of the exon junction complex. Genes Dev. 2004;18:210-22 pubmed
    ..Translational stimulation can be replicated by tethering the EJC proteins Y14, Magoh, and RNPS1 or the nonsense-mediated decay (NMD) factors Upf1, Upf2, and Upf3b to an intronless reporter mRNA...
  31. Gehring N, Kunz J, Neu Yilik G, Breit S, Viegas M, Hentze M, et al. Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements. Mol Cell. 2005;20:65-75 pubmed
    ..Specifically, Y14, MAGOH, and eIF4A3 can activate NMD in an UPF2-independent manner, whereas RNPS1-induced NMD requires UPF2...