RNF4

Summary

Gene Symbol: RNF4
Description: ring finger protein 4
Alias: RES4-26, SLX5, SNURF, E3 ubiquitin-protein ligase RNF4, E3 ubiquitin ligase RNF4, RING-type E3 ubiquitin transferase RNF4, small nuclear RING finger protein
Species: human
Products:     RNF4

Top Publications

  1. Galili N, Nayak S, Epstein J, Buck C. Rnf4, a RING protein expressed in the developing nervous and reproductive systems, interacts with Gscl, a gene within the DiGeorge critical region. Dev Dyn. 2000;218:102-11 pubmed
    ..The screen resulted in the identification of a RING protein known as rnf4 or snrf. Gscl/rnf4 interactions were confirmed by affinity chromatography and by immunoprecipitation...
  2. Tatham M, Geoffroy M, Shen L, Plechanovova A, Hattersley N, Jaffray E, et al. RNF4 is a poly-SUMO-specific E3 ubiquitin ligase required for arsenic-induced PML degradation. Nat Cell Biol. 2008;10:538-46 pubmed publisher
    ..Here we demonstrate that the RING-domain-containing ubiquitin E3 ligase, RNF4 (also known as SNURF), targets poly-SUMO-modified proteins for degradation mediated by ubiquitin...
  3. Prudden J, Pebernard S, Raffa G, Slavin D, Perry J, Tainer J, et al. SUMO-targeted ubiquitin ligases in genome stability. EMBO J. 2007;26:4089-101 pubmed
    ..pombe Slx8-Rfp (founding member), Homo sapiens RNF4, Dictyostelium discoideum MIP1 and Saccharomyces cerevisiae Slx5-Slx8...
  4. Tatham M, Plechanovová A, Jaffray E, Salmen H, Hay R. Ube2W conjugates ubiquitin to α-amino groups of protein N-termini. Biochem J. 2013;453:137-45 pubmed publisher
    ..modifier SUMO (small ubiquitin-related modifier) in a manner dependent on the SUMO-targeted ubiquitin ligase RNF4 (RING finger protein 4)...
  5. Keusekotten K, Bade V, Meyer Teschendorf K, Sriramachandran A, Fischer Schrader K, Krause A, et al. Multivalent interactions of the SUMO-interaction motifs in RING finger protein 4 determine the specificity for chains of the SUMO. Biochem J. 2014;457:207-14 pubmed publisher
    b>RNF4 (RING finger protein 4) is a STUbL [SUMO (small ubiquitin-related modifier)-targeted ubiquitin ligase] controlling PML (promyelocytic leukaemia) nuclear bodies, DNA double strand break repair and other nuclear functions...
  6. Geoffroy M, Jaffray E, Walker K, Hay R. Arsenic-induced SUMO-dependent recruitment of RNF4 into PML nuclear bodies. Mol Biol Cell. 2010;21:4227-39 pubmed publisher
    ..Here we analyze the nuclear trafficking dynamics of PML and its SUMO-dependent ubiquitin E3 ligase, RNF4 in response to arsenic...
  7. Guzzo C, Berndsen C, Zhu J, Gupta V, Datta A, Greenberg R, et al. RNF4-dependent hybrid SUMO-ubiquitin chains are signals for RAP80 and thereby mediate the recruitment of BRCA1 to sites of DNA damage. Sci Signal. 2012;5:ra88 pubmed publisher
    ..Furthermore, RNF4, a SUMO-targeted ubiquitin E3 ligase that synthesizes hybrid SUMO-ubiquitin chains, localized to DSBs and was ..
  8. Chiariotti L, Benvenuto G, Fedele M, Santoro M, Simeone A, Fusco A, et al. Identification and characterization of a novel RING-finger gene (RNF4) mapping at 4p16.3. Genomics. 1998;47:258-65 pubmed
    We have isolated a new human RING-finger gene (RNF4) that encodes a 190-amino-acid protein...
  9. Hakli M, Karvonen U, Janne O, Palvimo J. SUMO-1 promotes association of SNURF (RNF4) with PML nuclear bodies. Exp Cell Res. 2005;304:224-33 pubmed
    b>Small nuclear RING finger protein SNURF (RNF4) is involved in transcriptional and cell growth regulation...

More Information

Publications107 found, 100 shown here

  1. Mukhopadhyay D, Arnaoutov A, Dasso M. The SUMO protease SENP6 is essential for inner kinetochore assembly. J Cell Biol. 2010;188:681-92 pubmed publisher
    ..We further found that CENP-I is degraded through the action of RNF4, a ubiquitin ligase which targets polysumoylated proteins for proteasomal degradation, and that SENP6 stabilizes ..
  2. Galanty Y, Belotserkovskaya R, Coates J, Jackson S. RNF4, a SUMO-targeted ubiquitin E3 ligase, promotes DNA double-strand break repair. Genes Dev. 2012;26:1179-95 pubmed publisher
    ..Here, we show that human RNF4, a small ubiquitin-like modifier (SUMO)-targeted ubiquitin E3 ligase, is recruited to DSBs in a manner requiring ..
  3. Moilanen A, Poukka H, Karvonen U, Hakli M, Janne O, Palvimo J. Identification of a novel RING finger protein as a coregulator in steroid receptor-mediated gene transcription. Mol Cell Biol. 1998;18:5128-39 pubmed
    ..domain of androgen receptor (AR) as a bait in a yeast two-hybrid screening, we have identified a small nuclear RING finger protein, termed SNURF, that interacts with AR in a hormone-dependent fashion in both yeast and mammalian ..
  4. Poukka H, Aarnisalo P, Santti H, Janne O, Palvimo J. Coregulator small nuclear RING finger protein (SNURF) enhances Sp1- and steroid receptor-mediated transcription by different mechanisms. J Biol Chem. 2000;275:571-9 pubmed
    The small nuclear RING finger protein SNURF is not only a coactivator in steroid receptor-dependent transcription but also activates transcription from steroid-independent promoters...
  5. Hakli M, Lorick K, Weissman A, Janne O, Palvimo J. Transcriptional coregulator SNURF (RNF4) possesses ubiquitin E3 ligase activity. FEBS Lett. 2004;560:56-62 pubmed
    b>SNURF/RNF4 has been implicated in transcriptional regulation and growth inhibition in a RING finger-dependent fashion...
  6. Plechanovova A, Jaffray E, McMahon S, Johnson K, Navratilova I, Naismith J, et al. Mechanism of ubiquitylation by dimeric RING ligase RNF4. Nat Struct Mol Biol. 2011;18:1052-9 pubmed publisher
    Mammalian RNF4 is a dimeric RING ubiquitin E3 ligase that ubiquitylates poly-SUMOylated proteins. We found that RNF4 bound ubiquitin-charged UbcH5a tightly but free UbcH5a weakly...
  7. Weisshaar S, Keusekotten K, Krause A, Horst C, Springer H, Göttsche K, et al. Arsenic trioxide stimulates SUMO-2/3 modification leading to RNF4-dependent proteolytic targeting of PML. FEBS Lett. 2008;582:3174-8 pubmed publisher
    ..The SUMO-binding ubiquitin ligase RNF4 mediates this modification and causes disruption of PML nuclear bodies upon treatment with ATO...
  8. Bruderer R, Tatham M, Plechanovova A, Matic I, Garg A, Hay R. Purification and identification of endogenous polySUMO conjugates. EMBO Rep. 2011;12:142-8 pubmed publisher
    ..We use this approach to identify more than 300 putative polySUMO conjugates from cultured eukaryotic cells. ..
  9. Yin Y, Seifert A, Chua J, Maure J, Golebiowski F, Hay R. SUMO-targeted ubiquitin E3 ligase RNF4 is required for the response of human cells to DNA damage. Genes Dev. 2012;26:1196-208 pubmed publisher
    Here we demonstrate that RNF4, a highly conserved small ubiquitin-like modifier (SUMO)-targeted ubiquitin E3 ligase, plays a critical role in the response of mammalian cells to DNA damage...
  10. Sun H, Hunter T. Poly-small ubiquitin-like modifier (PolySUMO)-binding proteins identified through a string search. J Biol Chem. 2012;287:42071-83 pubmed publisher
    ..Like the small ubiquitin-like modifier (SUMO)-targeted ubiquitin ligase RNF4, proteins with clustered SUMO-interacting motifs (SIMs) can be important signal transducers downstream of ..
  11. Rojas Fernandez A, Plechanovová A, Hattersley N, Jaffray E, Tatham M, Hay R. SUMO chain-induced dimerization activates RNF4. Mol Cell. 2014;53:880-92 pubmed publisher
    ..b>RNF4 is an E3 ligase containing an N-terminal domain that binds its polySUMO substrates and a C-terminal RING domain ..
  12. Kaiser F, Moroy T, Chang G, Horsthemke B, Lüdecke H. The RING finger protein RNF4, a co-regulator of transcription, interacts with the TRPS1 transcription factor. J Biol Chem. 2003;278:38780-5 pubmed
    ..we obtained three yeast clones encoding two overlapping fragments of the 194 amino acids RING finger protein 4 (Rnf4). The overlap narrows down the Trps1-binding region within Rnf4 to amino acids 6-65...
  13. Percherancier Y, Germain Desprez D, Galisson F, Mascle X, Dianoux L, Estephan P, et al. Role of SUMO in RNF4-mediated promyelocytic leukemia protein (PML) degradation: sumoylation of PML and phospho-switch control of its SUMO binding domain dissected in living cells. J Biol Chem. 2009;284:16595-608 pubmed publisher
    ..PML sumoylation and proteasome-dependent degradation induced by the E3 ubiquitin ligase, RNF4, are enhanced by the acute promyelocytic leukemia therapeutic agent, arsenic trioxide (As2O3)...
  14. Garvin A, Walker A, Densham R, Chauhan A, Stone H, Mackay H, et al. The deSUMOylase SENP2 coordinates homologous recombination and nonhomologous end joining by independent mechanisms. Genes Dev. 2019;33:333-347 pubmed publisher
    ..We found that regulated deSUMOylation of MDC1 prevents excessive SUMOylation and its RNF4-VCP mediated clearance from DSBs, thereby promoting NHEJ...
  15. Hirota K, Tsuda M, Murai J, Takagi T, Keka I, Narita T, et al. SUMO-targeted ubiquitin ligase RNF4 plays a critical role in preventing chromosome loss. Genes Cells. 2014;19:743-54 pubmed publisher
    RING finger protein 4 (RNF4) represents a subclass of ubiquitin ligases that target proteins modified by the small ubiquitin-like modifier (SUMO) for ubiquitin-mediated degradation...
  16. Korbolina E, Ershov N, Bryzgalov L, Kolosova N. Application of quantitative trait locus mapping and transcriptomics to studies of the senescence-accelerated phenotype in rats. BMC Genomics. 2014;15 Suppl 12:S3 pubmed publisher
    ..Several candidate genes (including Arhgap33, Cebpg, Gtf3c1, Snurf, Tnfaip3, Yme1l1, Cbs, Car9 and Fn1) were found to be either polymorphic in the congenic loci or differentially ..
  17. Marinello M, Werner A, Giannone M, Tahiri K, Alves S, Tesson C, et al. SUMOylation by SUMO2 is implicated in the degradation of misfolded ataxin-7 via RNF4 in SCA7 models. Dis Model Mech. 2019;12: pubmed publisher
    ..Moreover, upon inhibition of the proteasome, both endogenous SUMO2/3 and the RNF4 ubiquitin ligase surround large polyQ-ATXN7 intranuclear inclusions...
  18. Dassouki Z, Sahin U, El Hajj H, Jollivet F, Kfoury Y, Lallemand Breitenbach V, et al. ATL response to arsenic/interferon therapy is triggered by SUMO/PML/RNF4-dependent Tax degradation. Blood. 2015;125:474-82 pubmed publisher
    ..PML-dependent hyper-sumoylation by small ubiquitin-like modifier (SUMO)2/3 but not SUMO1, ubiquitination by RNF4, and proteasome-dependent degradation...
  19. Kumar R, González Prieto R, Xiao Z, Verlaan de Vries M, Vertegaal A. The STUbL RNF4 regulates protein group SUMOylation by targeting the SUMO conjugation machinery. Nat Commun. 2017;8:1809 pubmed publisher
    ..In search of direct targets for the STUbL RNF4, we have developed TULIP (targets for ubiquitin ligases identified by proteomics) to covalently trap targets for ..
  20. Nakagaki A, Osanai H, Kishino T. Imprinting analysis of the mouse chromosome 7C region in DNMT1-null embryos. Gene. 2014;553:63-8 pubmed publisher
    ..The imprinted domain contains paternally expressed genes, Snurf/Snrpn, Ndn, Magel2, Mkrn3, and Frat3, C/D-box small nucleolar RNAs (snoRNAs), and the maternally expressed gene, ..
  21. Yang H, Chuang J, Jeng W, Liu C, Wang A, Lu P, et al. Pin1-mediated Sp1 phosphorylation by CDK1 increases Sp1 stability and decreases its DNA-binding activity during mitosis. Nucleic Acids Res. 2014;42:13573-87 pubmed publisher
    ..by CDK1 during mitosis not only stabilized Sp1 levels by decreasing interaction with ubiquitin E3-ligase RNF4 but also caused Sp1 to move out of the chromosomes completely by decreasing its DNA-binding activity, thereby ..
  22. Lin H, Li Y, Xing W, Qiu Q, Wang W, Zhang Q. Genome-wide screening differential long non-coding RNAs expression profiles discloses its roles involved in OHSS development. J Assist Reprod Genet. 2018;35:1473-1482 pubmed publisher
    ..05, FDR value < 0.001), of which 205 were upregulated and 277 were downregulated. Lnc-SEC16B.1-6, lnc-SNURF-13, lnc-LGR6-6, and lnc-H2AFY2-2 were up-regulated, while lnc-BRD2-2, lnc-HSPA6-2, and lnc-CLIC6-5 were ..
  23. Lyngsø C, Bouteiller G, Damgaard C, Ryom D, Sanchez Muñoz S, Nørby P, et al. Interaction between the transcription factor SPBP and the positive cofactor RNF4. An interplay between protein binding zinc fingers. J Biol Chem. 2000;275:26144-9 pubmed
    The activator of stromelysin 1 gene transcription, SPBP, interacts with the RING finger protein RNF4. Both proteins are ubiquitously expressed and localized in the nucleus...
  24. Gibbs Seymour I, Oka Y, Rajendra E, Weinert B, Passmore L, Patel K, et al. Ubiquitin-SUMO circuitry controls activated fanconi anemia ID complex dosage in response to DNA damage. Mol Cell. 2015;57:150-64 pubmed publisher
    ..ID complex drives substrate selectivity by triggering its polyubiquitylation by the SUMO-targeted ubiquitin ligase RNF4 to promote its removal from sites of DNA damage via the DVC1-p97 ubiquitin segregase complex...
  25. Bruno C, Blagoskonov O, Barberet J, Guilleman M, Daniel S, Tournier B, et al. Sperm imprinting integrity in seminoma patients?. Clin Epigenetics. 2018;10:125 pubmed publisher
    ..of imprinted genes [H19/IGF2: IG-DMR (CTCF3 and CTCF6 of H19 gene); IGF2-DMRs (DMR0 and DMR2); MEG3/DLK1:IG-DMR; SNURF:TSS-DMR; KCNQ1OT1:TSS-DMR] were assessed by pyrosequencing. All comparative analyses were adjusted for age...
  26. Hay R. Decoding the SUMO signal. Biochem Soc Trans. 2013;41:463-73 pubmed publisher
    ..This ubiquitin ligase known as RNF4 (RING finger protein 4) has the unique property that it can recognize SUMO-modified proteins and target them for ..
  27. Barrow T, Barault L, Ellsworth R, Harris H, Binder A, Valente A, et al. Aberrant methylation of imprinted genes is associated with negative hormone receptor status in invasive breast cancer. Int J Cancer. 2015;137:537-47 pubmed publisher
    ..38%, DIRAS3 DMR in 36%, GRB10 ICR in 23%, PEG3 DMR in 21%, MEST ICR in 19%, H19 ICR in 18%, KvDMR in 8% and SNRPN/SNURF ICR in 4%...
  28. Luo K, Deng M, Li Y, Wu C, Xu Z, Yuan J, et al. CDK-mediated RNF4 phosphorylation regulates homologous recombination in S-phase. Nucleic Acids Res. 2015;43:5465-75 pubmed publisher
    ..Here we reported that CDK2 could phosphorylate RNF4 on T26 and T112 and enhance RNF4 E3 ligase activity, which is important for MDC1 degradation and proper HR repair ..
  29. Xu W, Liu S, Wu D, Qiao G, Yan J. Sumoylation of the Tumor Suppressor Promyelocytic Leukemia Protein Regulates Arsenic Trioxide-Induced Collagen Synthesis in Osteoblasts. Cell Physiol Biochem. 2015;37:1581-91 pubmed publisher
    ..hFOB1.19 cells were transfected with siRNA against UBC9 or RNF4, and then treated with ATO or FBS...
  30. Di Costanzo A, Del Gaudio N, Conte L, Dell aversana C, Vermeulen M, De The H, et al. The HDAC inhibitor SAHA regulates CBX2 stability via a SUMO-triggered ubiquitin-mediated pathway in leukemia. Oncogene. 2018;37:2559-2572 pubmed publisher
    ..We identify CBX4 and RNF4 as the E3 SUMO and E3 ubiquitin ligase, respectively, and describe the specific molecular mechanism regulating CBX2 ..
  31. Liu Y, Li D, Nie D, Liu S, Qiu F, Liu M, et al. Arsenic trioxide and angiotensin II have inhibitory effects on HERG protein expression: Evidence for the role of PML SUMOylation. Oncotarget. 2017;8:45447-45458 pubmed publisher
    ..Conversely, knockdown of RNF4 led to a remarkable increase in PML SUMOylation and the function of PML-NBs, further promoting ATO- or Ang II-..
  32. Hadano S, Ishida Y, Ikeda J. The primary structure and genomic organization of five novel transcripts located close to the Huntington's disease gene on human chromosome 4p16.3. DNA Res. 1998;5:177-86 pubmed
    ..The transcript map based upon our current level of analysis combined with data from previous studies reveals the gene-rich nature and the intricate organization of the genes in the HD locus. ..
  33. Shao X, Zhang R, Hu C, Wang C, Lu J, Qin W, et al. Precise microdeletion detection of Prader-Willi Syndrome with array comparative genome hybridization. Biomed Environ Sci. 2010;23:194-8 pubmed publisher
    ..22 Mb type II microdeletion was detected, covering a region from MKRN3, MAGEL2, NDN, PWRN2, PWRN1, C12orf2, SNURF-SNRPN, C/D snoRNAs, to distal of UBE3A...
  34. Müncheberg S, Hay R, Ip W, Meyer T, Weis C, Brenke J, et al. E1B-55K-Mediated Regulation of RNF4 SUMO-Targeted Ubiquitin Ligase Promotes Human Adenovirus Gene Expression. J Virol. 2018;92: pubmed publisher
    ..b>RNF4, a cellular SUMO-targeted ubiquitin ligase (STUbL), induces ubiquitinylation of specific SUMOylated proteins and ..
  35. Hendriks I, Treffers L, Verlaan de Vries M, Olsen J, Vertegaal A. SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage. Cell Rep. 2015;10:1778-1791 pubmed publisher
    ..Whereas SUMOylated JARID1B was ubiquitylated by the SUMO-targeted ubiquitin ligase RNF4 and degraded by the proteasome in response to DNA damage, JARID1C was SUMOylated and recruited to the chromatin to ..
  36. Cao Y, AlHumaidi S, Faqeih E, Pitel B, Lundquist P, Aypar U. A novel deletion of SNURF/SNRPN exon 1 in a patient with Prader-Willi-like phenotype. Eur J Med Genet. 2017;60:416-420 pubmed publisher
    Here we report the smallest deletion involving SNURF/SNRPN that causes major symptoms of Prader-Willi syndrome (PWS), including hypotonia, dysmorphic features, intellectual disability, and obesity...
  37. Sahin U, Ferhi O, Jeanne M, Benhenda S, Berthier C, Jollivet F, et al. Oxidative stress-induced assembly of PML nuclear bodies controls sumoylation of partner proteins. J Cell Biol. 2014;204:931-45 pubmed publisher
    ..Some NB-associated sumoylated partners also become polyubiquitinated by RNF4, precipitating their proteasomal degradation...
  38. Hassan M, Butler M. Prader-Willi syndrome and atypical submicroscopic 15q11-q13 deletions with or without imprinting defects. Eur J Med Genet. 2016;59:584-589 pubmed publisher
    ..MLPA analysis, we updated the genetic findings on our patient and found a 209,819bp deletion including the SNURF-SNRPN gene complex which includes the imprinting center and the SNORD116 region...
  39. Plechanovová A, Jaffray E, Tatham M, Naismith J, Hay R. Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis. Nature. 2012;489:115-20 pubmed publisher
    ..Here we report the crystal structure of the dimeric RING domain of rat RNF4 in complex with E2 (UbcH5A) linked by an isopeptide bond to ubiquitin...
  40. Wang Y, Yang W, Chang W, Hung J. Interplay of posttranslational modifications in Sp1 mediates Sp1 stability during cell cycle progression. J Mol Biol. 2011;414:1-14 pubmed publisher
    ..Here, we identified RNF4 as the ubiquitin E3 ligase of Sp1...
  41. Wang M, Sang J, Ren Y, Liu K, Liu X, Zhang J, et al. SENP3 regulates the global protein turnover and the Sp1 level via antagonizing SUMO2/3-targeted ubiquitination and degradation. Protein Cell. 2016;7:63-77 pubmed publisher
    ..b>RNF4 is the most studied human SUMO-targeted ubiquitin E3 ligase...
  42. Kuo C, Li X, Stark J, Shih H, Ann D. RNF4 regulates DNA double-strand break repair in a cell cycle-dependent manner. Cell Cycle. 2016;15:787-98 pubmed publisher
    Both RNF4 and KAP1 play critical roles in the response to DNA double-strand breaks (DSBs), but the functional interplay of RNF4 and KAP1 in regulating DNA damage response remains unclear...
  43. Yang Y, Yoshikai Y, Hsu S, Saitoh H, Chang L. Role of RNF4 in the ubiquitination of Rta of Epstein-Barr virus. J Biol Chem. 2013;288:12866-79 pubmed publisher
    ..GST pulldown and coimmunoprecipitation studies reveal that RNF4, a RING-domain-containing ubiquitin E3 ligase, interacts with Rta...
  44. Gong X, Ahner A, Roldan A, Lukacs G, Thibodeau P, Frizzell R. Non-native Conformers of Cystic Fibrosis Transmembrane Conductance Regulator NBD1 Are Recognized by Hsp27 and Conjugated to SUMO-2 for Degradation. J Biol Chem. 2016;291:2004-17 pubmed publisher
    ..E2 enzyme for protein SUMOylation, to selectively degrade F508del CFTR via the SUMO-targeted ubiquitin E3 ligase, RNF4 (RING finger protein 4) (1)...
  45. Fasci D, Anania V, Lill J, Salvesen G. Response to Comment on "SUMO deconjugation is required for arsenic-triggered ubiquitylation of PML". Sci Signal. 2016;9:tc2 pubmed publisher
    ..We proposed that the SUMO switch at Lys(65) of PML enhanced subsequent SUMO2 conjugation to Lys(160) and consequent RNF4-dependent ubiquitylation of PML...
  46. Sarkari F, Wang X, Nguyen T, Frappier L. The herpesvirus associated ubiquitin specific protease, USP7, is a negative regulator of PML proteins and PML nuclear bodies. PLoS ONE. 2011;6:e16598 pubmed publisher
    ..USP7 also disrupted NBs formed from some single PML isoforms, most notably isoforms I and IV. CK2α and RNF4, which are known regulators of PML, were dispensable for USP7-associated PML-NB disruption...
  47. Maroui M, Kheddache Atmane S, El Asmi F, Dianoux L, Aubry M, Chelbi Alix M. Requirement of PML SUMO interacting motif for RNF4- or arsenic trioxide-induced degradation of nuclear PML isoforms. PLoS ONE. 2012;7:e44949 pubmed publisher
    ..trioxide (As?O?) enhances PML SUMOylation leading to an increase in PML NB size and promotes its interaction with RNF4, a poly-SUMO-dependent ubiquitin E3 ligase responsible for proteasome-mediated PML degradation...
  48. Wu Y, Hwang Y, Perng W, Chian C, Ho C, Lee S, et al. CPEB4 and IRF4 expression in peripheral mononuclear cells are potential prognostic factors for advanced lung cancer. J Formos Med Assoc. 2017;116:114-122 pubmed publisher
    ..and real-time polymerase chain reaction (PCR) for the expression profiling of eight genes: DUSP6, MMD, CPEB4, RNF4, STAT2, NF1, IRF4, and ZNF264...
  49. Maure J, Moser S, Jaffray E, F Alpi A, Hay R. Loss of ubiquitin E2 Ube2w rescues hypersensitivity of Rnf4 mutant cells to DNA damage. Sci Rep. 2016;6:26178 pubmed publisher
    ..These pathways are linked by the SUMO Targeted ubiquitin Ligase Rnf4 that catalyses transfer of ubiquitin from a ubiquitin loaded E2 conjugating enzyme to a polySUMO modified substrate...
  50. Groocock L, Nie M, Prudden J, Moiani D, Wang T, Cheltsov A, et al. RNF4 interacts with both SUMO and nucleosomes to promote the DNA damage response. EMBO Rep. 2014;15:601-8 pubmed publisher
    ..The ubiquitin ligase RNF4 integrates signaling by SUMO and ubiquitin, through its selective recognition and ubiquitination of SUMO-modified ..
  51. Wang Y. RING finger protein 4 (RNF4) derepresses gene expression from DNA methylation. J Biol Chem. 2014;289:33808-13 pubmed publisher
    b>RNF4 is an E3 ubiquitin ligase originally identified as a transcription co-activator. The mechanism by which RNF4 promotes transcription remains unclear...
  52. Fasci D, Anania V, Lill J, Salvesen G. SUMO deconjugation is required for arsenic-triggered ubiquitylation of PML. Sci Signal. 2015;8:ra56 pubmed publisher
    ..of two SUMO paralogs (SUMO1 and SUMO2) to promote PML ubiquitylation mediated by the ubiquitin E3 ligase RNF4 and reorganization of PML nuclear bodies...
  53. Liang Y, Lee C, Yao Y, Lai C, Schmitz M, Yang W. SUMO5, a Novel Poly-SUMO Isoform, Regulates PML Nuclear Bodies. Sci Rep. 2016;6:26509 pubmed publisher
    ..SUMO5 also increases polySUMO2/3 conjugation of PML, resulting in RNF4-mediated disruption of PML-NBs...
  54. Koufaris C, Alexandrou A, Papaevripidou I, Alexandrou I, Christophidou Anastasiadou V, Sismani C. Deletion of SNURF/SNRPN U1B and U1B* upstream exons in a child with developmental delay and excessive weight. J Genet. 2016;95:621-4 pubmed
    ..that he was a carrier of a paternally inherited deletion that affected the U1B and U1B* upstream exons of the SNURF-SNRNP gene within the 15q11-q13 imprinted region...
  55. Chen P, Hsiao J, Sirois C, Chamberlain S. RBFOX1 and RBFOX2 are dispensable in iPSCs and iPSC-derived neurons and do not contribute to neural-specific paternal UBE3A silencing. Sci Rep. 2016;6:25368 pubmed publisher
    ..UBE3A-ATS transcript in neurons, but are not required for gene expression and/or neuron-specific processing in the SNURF/SNRPN-UBE3A region...
  56. Choi H, Kwon S, Kim D. A post-translational modification cascade employing HDAC9-PIASy-RNF4 axis regulates chondrocyte hypertrophy by modulating Nkx3.2 protein stability. Cell Signal. 2016;28:1336-48 pubmed publisher
    ..2 triggers PIASy-mediated sumoylation and subsequent RNF4-mediated SUMO-targeted ubiquitination. Furthermore, we demonstrate that Nkx3...
  57. Liu Y, Zhao D, Qiu F, Zhang L, Liu S, Li Y, et al. Manipulating PML SUMOylation via Silencing UBC9 and RNF4 Regulates Cardiac Fibrosis. Mol Ther. 2017;25:666-678 pubmed publisher
    ..In contrast, enhancing SUMOylated PML accumulation, by silencing RNF4, a poly-SUMO-specific E3 ubiquitin ligase, accelerated the induction of cardiac fibrosis and promoted cardiac ..
  58. Iyer R, Chatham L, Sleigh R, Meek D. A functional SUMO-motif in the active site of PIM1 promotes its degradation via RNF4, and stimulates protein kinase activity. Sci Rep. 2017;7:3598 pubmed publisher
    ..SUMOylation promoted ubiquitin-mediated degradation of PIM1 via recruitment of the SUMO-targeted ubiquitin ligase, RNF4. Additionally, SUMOylated PIM1 showed enhanced protein kinase activity in vitro...
  59. Guo L, Giasson B, Glavis Bloom A, Brewer M, Shorter J, Gitler A, et al. A cellular system that degrades misfolded proteins and protects against neurodegeneration. Mol Cell. 2014;55:15-30 pubmed publisher
    ..Here we show that PML/TRIM19 and the SUMO-dependent ubiquitin ligase RNF4 act together to promote the degradation of misfolded proteins in the mammalian cell nucleus...
  60. Hu X, Liu Z, Zhang K, Feng J, Liu X, Wang L, et al. SUMO-specific protease 2-mediated deSUMOylation is required for NDRG2 stabilization in gastric cancer cells. Cancer Biomark. 2017;: pubmed publisher
    ..residue, which promoted its ubiquitination and sequentially degradation by the SUMO-targeted ubiquitin E3 ligase RNF4. However, how to regulated NDRG2 deSUMOylation process remains largely unknown...
  61. Fedele M, Benvenuto G, Pero R, Majello B, Battista S, Lembo F, et al. A novel member of the BTB/POZ family, PATZ, associates with the RNF4 RING finger protein and acts as a transcriptional repressor. J Biol Chem. 2000;275:7894-901 pubmed
    We have identified a novel human gene encoding a 59-kDa POZ-AT hook-zinc finger protein (PATZ) that interacts with RNF4, a mediator of androgen receptor activity, and acts as a transcriptional repressor...
  62. Hakli M, Karvonen U, Janne O, Palvimo J. The RING finger protein SNURF is a bifunctional protein possessing DNA binding activity. J Biol Chem. 2001;276:23653-60 pubmed
    The small nuclear C(3)HC(4) finger protein (SNURF), RNF4, acts as transcriptional coactivator for both steroid-dependent and -independent promoters such as those driven by androgen response elements and GC boxes, respectively...
  63. Banka P, Behera A, Sarkar S, Datta A. RING E3-Catalyzed E2 Self-Ubiquitination Attenuates the Activity of Ube2E Ubiquitin-Conjugating Enzymes. J Mol Biol. 2015;427:2290-304 pubmed publisher
    ..Based on these results, we propose that E3-catalyzed self-ubiquitination acts as a key regulatory mechanism that controls the activity of Ube2E class of ubiquitin E2s. ..
  64. LI Y, Perkins A, Su Y, Ma Y, Colson L, Horne D, et al. Gold nanoparticles as a platform for creating a multivalent poly-SUMO chain inhibitor that also augments ionizing radiation. Proc Natl Acad Sci U S A. 2012;109:4092-7 pubmed publisher
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    ..Interestingly, Arkadia homodimerizes but does not heterodimerize with RNF4, the other STUBL involved in PML degradation, suggesting that these two E3 ligases do not act synergistically but ..
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    ..residue K921, resulting in E2 SUMO-conjugating enzyme UBC9-mediated SUMOylation, RING finger protein 4-mediated (RNF4-mediated) polyubiquitination, and proteasome-mediated degradation of FANCA...
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    ..Knocking down the SUMO-targeted ubiquitin ligase RNF4 enhanced the levels of SUMOylated c-Myc, indicating that RNF4 could recognize a multi-SUMOylated protein as a ..
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    ..Here, we report that ubiquitylation by RNF4 stabilizes otherwise short-lived oncogenic transcription factors, including β-catenin, Myc, c-Jun, and the Notch ..
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    The RING-finger protein SNURF/RNF4, a modulator of both steroid receptor dependent and basal transcription, is expressed at very high levels in testis and at much lower levels in several other tissues...
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    ..Oestrogen receptors (ERalpha and ERbeta) and their co-regulators, including small nuclear ring finger protein 4 (SNURF/RNF4) mediate oestrogen actions...
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    ..of the SUMO-conjugation machinery and post-transcriptional downregulation of the SUMO-targeted ubiquitin ligase RNF4. The latter effect was mediated by selective inhibition of RNF4 protein expression by the viral miR-BHRF1-1...
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    A small nuclear RING finger protein, termed SNURF (or RNF4), is a coregulator of androgen receptor-dependent transcription...
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    ..Thus, the interactions of SLX4 with SUMO and ubiquitin increase its affinity for factors recognizing different DNA lesions or telomeres, helping to direct the SLX4 complex in distinct functional contexts. ..
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    Ring finger protein 4 (RNF4) is a SUMO-targeted ubiquitin E3 ligase with a pivotal function in the DNA damage response (DDR)...
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    ..With this library, we identified several candidate regulators of canonical NF-κB pathway, including RNF4. Overexpression of RNF4 impaired NF-κB activation in a dose-dependent manner, whereas RNF4 knockdown potentiated ..
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    ..We found that RSV-induced NRF2 degradation occurs in an SUMO-specific E3 ubiquitin ligase - RING finger protein 4 (RNF4)-dependent manner...
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    b>SNURF is a small RING finger protein that binds the zinc finger region of steroid hormone receptors and enhances Sp1- and androgen receptor-mediated transcription in COS and CV-1 cells...
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    PATZ is a transcriptional repressor affecting the basal activity of different promoters, whereas RNF4 is a transcriptional activator. The association of PATZ with RNF4 switches the activation to repression of selected basal promoters...
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    ..The ubiquitin ligase RNF4 integrates signaling by SUMO and ubiquitin through its selective recognition and ubiquitination of SUMO-modified ..
  82. Koidl S, Eisenhardt N, Fatouros C, Droescher M, Chaugule V, Pichler A. The SUMO2/3 specific E3 ligase ZNF451-1 regulates PML stability. Int J Biochem Cell Biol. 2016;79:478-487 pubmed publisher
    ..Our data suggest a regulatory role of ZNF451-1 in fine-tuning physiological PML levels in a RNF4 cooperative manner in the mouse neuroblastoma N2a cell-line.
  83. Sharma P, Mullen J, Li M, Zaratiegui M, Bunting S, Brill S. A Lysine Desert Protects a Novel Domain in the Slx5-Slx8 SUMO Targeted Ub Ligase To Maintain Sumoylation Levels in Saccharomyces cerevisiae. Genetics. 2017;206:1807-1821 pubmed publisher
    ..in genes known to control global sumoylation levels including ULP1, ULP2, SIZ2, and SLX5 The results suggest that alternative pathways of repair become available when sumoylation levels are altered...
  84. Kuo C, Li X, Kong X, Luo C, Chang C, Chung Y, et al. An arginine-rich motif of ring finger protein 4 (RNF4) oversees the recruitment and degradation of the phosphorylated and SUMOylated Krüppel-associated box domain-associated protein 1 (KAP1)/TRIM28 protein during genotoxic stress. J Biol Chem. 2014;289:20757-72 pubmed publisher
    ..the recruitment of small ubiquitin-like modifier (SUMO)-targeted ubiquitin E3 ligase, ring finger protein 4 (RNF4), and subsequent RNF4-mediated, SUMO-dependent degradation...
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  86. Bilodeau S, Caron V, Gagnon J, Kuftedjian A, Tremblay A. A CK2-RNF4 interplay coordinates non-canonical SUMOylation and degradation of nuclear receptor FXR. J Mol Cell Biol. 2017;9:195-208 pubmed publisher
    ..We also determine that such SUMOylation-dependent ubiquitination of FXR is mediated by the E3 ubiquitin ligase RNF4, which is required to achieve maximal induction of FXR and optimal up- or downregulation of responsive ..
  87. Kost L, Mootz H. A FRET sensor to monitor bivalent SUMO-SIM interactions in SUMO chain binding. Chembiochem. 2017;: pubmed publisher
    ..Alteration of the short linker sequence between the SIMs 2 and 3 of RNF4 showed a great tolerance, and hence structural flexibility, of the SUMO dimer for bivalent binding of adjacent SIMs...
  88. Hu X, Rodrigues T, Tao H, Baker R, Miraglia L, Orth A, et al. Identification of RING finger protein 4 (RNF4) as a modulator of DNA demethylation through a functional genomics screen. Proc Natl Acad Sci U S A. 2010;107:15087-92 pubmed publisher
    ..Here we show that RNF4 (RING finger protein 4), a SUMO-dependent ubiquitin E3-ligase previously implicated in maintaining genome stability,..
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    The E3 ubiquitin ligase RNF4 (RING finger protein 4) contains four tandem SIM [SUMO (small ubiquitin-like modifier)-interaction motif] repeats for selective interaction with poly-SUMO-modified proteins, which it targets for degradation...
  90. Malloy M, McIntosh D, Walters T, Flores A, Goodwin J, Arinze I. Trafficking of the transcription factor Nrf2 to promyelocytic leukemia-nuclear bodies: implications for degradation of NRF2 in the nucleus. J Biol Chem. 2013;288:14569-83 pubmed publisher
    ..of PML-NBs did not contain biologically active Keap1 but contained modified Nrf2 as well as RING finger protein 4 (RNF4), a poly-SUMO-specific E3 ubiquitin ligase...
  91. Lecona E, Rodriguez Acebes S, Specks J, López Contreras A, Ruppen I, Murga M, et al. USP7 is a SUMO deubiquitinase essential for DNA replication. Nat Struct Mol Biol. 2016;23:270-7 pubmed publisher
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    ..to be co-modified by SUMO and ubiquitin on inhibition of the proteasome and subsequently identified as novel RNF4 targets...