RNASEH2C

Summary

Gene Symbol: RNASEH2C
Description: ribonuclease H2, subunit C
Alias: AGS3, AYP1, RNase H1 small subunit, RNase H2 subunit C, aicardi-Goutieres syndrome 3 protein, ribonuclease H2 subunit C, ribonuclease HI subunit C
Species: human

Top Publications

  1. pmc Activator of G protein signaling 3 is a guanine dissociation inhibitor for Galpha i subunits
    L de Vries
    Departments of Cellular and Molecular Medicine and Pathology, University of California at San Diego, La Jolla, CA 92093 0651, USA
    Proc Natl Acad Sci U S A 97:14364-9. 2000
  2. pmc Distribution of activator of G-protein signaling 3 within the aggresomal pathway: role of specific residues in the tetratricopeptide repeat domain and differential regulation by the AGS3 binding partners Gi(alpha) and mammalian inscuteable
    Ali Vural
    Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Mol Cell Biol 30:1528-40. 2010
  3. pmc Clinical and molecular phenotype of Aicardi-Goutieres syndrome
    Gillian Rice
    Leeds Institute of Molecular Medicine, St James s University Hospital, Leeds, LS9 7TF, UK
    Am J Hum Genet 81:713-25. 2007
  4. ncbi Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutières syndrome and mimic congenital viral brain infection
    Yanick J Crow
    Leeds Institute of Molecular Medicine, University of Leeds, St James s University Hospital, Leeds, LS9 7TF, UK
    Nat Genet 38:910-6. 2006
  5. ncbi The presence of a Leu-Gly-Asn repeat-enriched protein (LGN), a putative binding partner of transducin, in ROD photoreceptors
    K Saidas Nair
    Department of Molecular and Cellular Pharmacology and Neuroscience Program, University of Miami School of Medicine, Miami, Florida 33136, USA
    Invest Ophthalmol Vis Sci 46:383-9. 2005
  6. ncbi AGS3: a G-Protein regulator of addiction-associated behaviors
    M S Bowers
    Department of Physiology and Neuroscience, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Ann N Y Acad Sci 1003:356-7. 2003
  7. pmc Deletion of the α-(1,3)-glucan synthase genes induces a restructuring of the conidial cell wall responsible for the avirulence of Aspergillus fumigatus
    Anne Beauvais
    Unite des Aspergillus, Institut Pasteur, Paris, France
    PLoS Pathog 9:e1003716. 2013
  8. ncbi Genomics, morphogenesis and biophysics: triangulation of Purkinje cell development
    Malcolm J Simons
    Haplomic Technologies Pty Ltd, Victoria, Australia
    Cerebellum 5:27-35. 2006
  9. ncbi Analyses of Galpha-interacting protein and activator of G-protein-signaling-3 functions in macroautophagy
    Sophie Pattingre
    Division of Infectious Diseases, Southwestern Medical School, Dallas, Texas 75390, USA
    Methods Enzymol 390:17-31. 2004
  10. ncbi Purification and in vitro functional analyses of RGS12 and RGS14 GoLoco motif peptides
    Randall J Kimple
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, The University of North Carolina, Chapel Hill 27599, USA
    Methods Enzymol 390:416-36. 2004

Scientific Experts

  • M S Bowers
  • Julie Vogt
  • Peter Kalivas
  • Anne Beauvais
  • Stefano D'Arrigo
  • M Schwendt
  • Yanick J Crow
  • Lina Yao
  • Jean Marc Taymans
  • S Ferre
  • Monica Gotta
  • Francis S Willard
  • Peter Bryant
  • Stephen M Lanier
  • Mikel Garcia-Marcos
  • Graham A Bowmaker
  • Ivan Diamond
  • Joe B Blumer
  • Quansheng Du
  • Lori Knackstedt
  • JOE BLUMER
  • MICHELER RICHARDSON
  • Micheler Ricardo Richardson
  • Sachiko Kamakura
  • Ali Vural
  • Ningfei An
  • Dzwokai Ma
  • Benjamin Groves
  • Hideki Sumimoto
  • Sophie Pattingre
  • Gillian I Rice
  • Jason M Conley
  • Celine Yeh
  • Wei Wang
  • Sukru S Oner
  • Bonnie O Leung
  • Michael L Bernard
  • Junya Hayase
  • Yuko Iwakiri
  • Anirban Adhikari
  • Christine Henry
  • Adam D Werts
  • Kevin R Regner
  • Satoru Yuzawa
  • Scott E Williams
  • Martin A M Reijns
  • M L Bernard
  • William E Sweeney
  • Y K Peterson
  • Frank Park
  • Simona Orcesi
  • Ronen Spiegel
  • Pierre Lebon
  • Elisa Fazzi
  • Blanca Gener
  • John H Livingston
  • Zhuojin Xu
  • Stavit A Shalev
  • Agathe Roubertie
  • Sukru Sadik Oner
  • Kate E Chandler
  • Alec Aeby
  • Rama Nadella
  • Corinne De Laet
  • Jeffrey Bailey
  • Doriette Soler
  • Magnhild Rasmussen
  • Adeline Vanderver
  • Ying Zhao
  • Yuri K Peterson
  • Hyongi Chon
  • R Sitarz
  • Peidong Fan
  • Christopher A Johnston
  • Andrew P Jackson
  • Daniele E Ejzykowicz
  • Celestine J Thomas
  • Anuradha Dhingra
  • B Groves
  • Gillian Rice
  • D Maubon
  • Malcolm J Simons
  • Thomas W Gettys
  • Tomoko Izaki
  • Stephen R Sprang
  • Kamon Sanada
  • Vasiliy S Kerov
  • K Saidas Nair
  • A Takesono
  • Tannin J Fuja

Detail Information

Publications76

  1. pmc Activator of G protein signaling 3 is a guanine dissociation inhibitor for Galpha i subunits
    L de Vries
    Departments of Cellular and Molecular Medicine and Pathology, University of California at San Diego, La Jolla, CA 92093 0651, USA
    Proc Natl Acad Sci U S A 97:14364-9. 2000
    Activator of G protein signaling 3 (AGS3) is a newly identified protein shown to act at the level of the G protein itself. AGS3 belongs to the GoLoco family of proteins, sharing the 19-aa GoLoco motif that is a Galpha(i/o) binding motif...
  2. pmc Distribution of activator of G-protein signaling 3 within the aggresomal pathway: role of specific residues in the tetratricopeptide repeat domain and differential regulation by the AGS3 binding partners Gi(alpha) and mammalian inscuteable
    Ali Vural
    Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Mol Cell Biol 30:1528-40. 2010
    b>AGS3, a receptor-independent activator of G-protein signaling, is involved in unexpected functional diversity for G-protein signaling systems...
  3. pmc Clinical and molecular phenotype of Aicardi-Goutieres syndrome
    Gillian Rice
    Leeds Institute of Molecular Medicine, St James s University Hospital, Leeds, LS9 7TF, UK
    Am J Hum Genet 81:713-25. 2007
    ..Biallelic mutations in TREX1, RNASEH2A, RNASEH2B, and RNASEH2C were observed in 31, 3, 47, and 18 families, respectively...
  4. ncbi Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutières syndrome and mimic congenital viral brain infection
    Yanick J Crow
    Leeds Institute of Molecular Medicine, University of Leeds, St James s University Hospital, Leeds, LS9 7TF, UK
    Nat Genet 38:910-6. 2006
    ..Our findings demonstrate a role for ribonuclease H in human neurological disease and suggest an unanticipated relationship between ribonuclease H2 and the antiviral immune response that warrants further investigation...
  5. ncbi The presence of a Leu-Gly-Asn repeat-enriched protein (LGN), a putative binding partner of transducin, in ROD photoreceptors
    K Saidas Nair
    Department of Molecular and Cellular Pharmacology and Neuroscience Program, University of Miami School of Medicine, Miami, Florida 33136, USA
    Invest Ophthalmol Vis Sci 46:383-9. 2005
    ..b>AGS3 and LGN contain four characteristic G protein regulator (GPR) domains that are responsible for its GDI function...
  6. ncbi AGS3: a G-Protein regulator of addiction-associated behaviors
    M S Bowers
    Department of Physiology and Neuroscience, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Ann N Y Acad Sci 1003:356-7. 2003
  7. pmc Deletion of the α-(1,3)-glucan synthase genes induces a restructuring of the conidial cell wall responsible for the avirulence of Aspergillus fumigatus
    Anne Beauvais
    Unite des Aspergillus, Institut Pasteur, Paris, France
    PLoS Pathog 9:e1003716. 2013
    ..There are three genes (AGS1, AGS2 and AGS3) controlling the biosynthesis of α-(1,3)-glucan in this fungal species...
  8. ncbi Genomics, morphogenesis and biophysics: triangulation of Purkinje cell development
    Malcolm J Simons
    Haplomic Technologies Pty Ltd, Victoria, Australia
    Cerebellum 5:27-35. 2006
    ..than 600 residues and including 2-3 GoLoco domains, designated as having G protein signaling modulator function (AGS3-like proteins)...
  9. ncbi Analyses of Galpha-interacting protein and activator of G-protein-signaling-3 functions in macroautophagy
    Sophie Pattingre
    Division of Infectious Diseases, Southwestern Medical School, Dallas, Texas 75390, USA
    Methods Enzymol 390:17-31. 2004
    ..the rate of autophagy, the role of two regulators of Gi3 protein activity, activator of G-protein-signaling-3 (AGS3) and Galpha-interacting protein (GAIP), was studied in the control of autophagy in human colon cancer HT-29 cells...
  10. ncbi Purification and in vitro functional analyses of RGS12 and RGS14 GoLoco motif peptides
    Randall J Kimple
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, The University of North Carolina, Chapel Hill 27599, USA
    Methods Enzymol 390:416-36. 2004
    ..proteins type 12 (RGS12) and 14 (RGS14) and a consensus GoLoco sequence from the multiple GoLoco motif protein AGS3. Three different fluorescence-based assays are described for evaluating the in vitro function of these GoLoco ..
  11. ncbi GoLoco motif peptides as probes of Galpha subunit specificity in coupling of G-protein-coupled receptors to ion channels
    Gerry S Oxford
    Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis 46202, USA
    Methods Enzymol 390:437-50. 2004
    ..Peptides encoding the GoLoco motifs of RGS12 and AGS3 were perfused into single cells during electrical recording of agonist-induced current responses by whole cell ..
  12. pmc Silver(I) complex formation with cysteine, penicillamine, and glutathione
    Bonnie O Leung
    Department of Chemistry, University of Calgary, Calgary, Alberta T2N 1N4, Canada
    Inorg Chem 52:4593-602. 2013
    ..47 ± 0.02 Å and δ((109)Ag) 1103 ppm, consistent with prevailing, partially oligomeric AgS3 coordinated species, while for penicillamine (C(Ag(I)) = 0.5 mol dm(-3), L/Ag = 2...
  13. pmc Differential effects of AGS3 expression on D(2L) dopamine receptor-mediated adenylyl cyclase signaling
    Jason M Conley
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 575 Stadium Mall Drive, West Lafayette, IN 47907, USA
    Cell Mol Neurobiol 33:551-8. 2013
    Activator of G protein signaling 3 (AGS3) binds Gα(i) subunits in the GDP-bound state, implicating AGS3 as an important regulator of Gα(i)-linked receptor (e.g., D2 dopamine and μ-opioid) signaling...
  14. doi Assessment of interferon-related biomarkers in Aicardi-Goutières syndrome associated with mutations in TREX1, RNASEH2A, RNASEH2B, RNASEH2C, SAMHD1, and ADAR: a case-control study
    Gillian I Rice
    Manchester Centre for Genomic Medicine, Institute of Human Development, Faculty of Medical and Human Sciences, University of Manchester, Manchester Academic Health Sciences Centre MAHSC, Manchester, UK
    Lancet Neurol 12:1159-69. 2013
    ..syndrome (AGS) is an inflammatory disorder caused by mutations in any of six genes (TREX1, RNASEH2A, RNASEH2B, RNASEH2C, SAMHD1, and ADAR). The disease is severe and effective treatments are urgently needed...
  15. pmc IGF-1 activates a cilium-localized noncanonical Gβγ signaling pathway that regulates cell-cycle progression
    Celine Yeh
    Margaret M Dyson Vision Research Institute, Department of Ophthalmology, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10065, USA
    Dev Cell 26:358-68. 2013
    ..At the base of the cilium, phosphorylated IGF-1R activates an AGS3-regulated Gβγ signaling pathway that subsequently recruits phospho(T94)Tctex-1 to the transition zone...
  16. doi The cell polarity protein mInsc regulates neutrophil chemotaxis via a noncanonical G protein signaling pathway
    Sachiko Kamakura
    Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
    Dev Cell 26:292-302. 2013
    ..neutrophils to recruit mInsc-complexed with the Par3-aPKC evolutionarily conserved polarity complex-via LGN/AGS3 that simultaneously binds to Gαi-GDP and mInsc...
  17. doi Striking intrafamilial phenotypic variability in Aicardi-Goutières syndrome associated with the recurrent Asian founder mutation in RNASEH2C
    Julie Vogt
    Centre for Rare Diseases and Personalised Medicine, Department of Medical and Molecular Genetics, School of Clinical and Experimental Medicine, University of Birmingham, Birmingham, UK
    Am J Med Genet A 161:338-42. 2013
    ..In contrast, the recurrent c.205C > T (p.R69W) RNASEH2C Asian founder mutation has previously only been identified in children with a severe AGS phenotype...
  18. doi Increased expression of AGS3 in rat brain cortex after traumatic brain injury
    Wei Wang
    Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, People s Republic of China
    J Neurosci Res 91:726-36. 2013
    ..Previous studies have demonstrated that AGS3, which belongs to the AGS family, is involved in a number of different cellular activities...
  19. pmc Translocation of activator of G-protein signaling 3 to the Golgi apparatus in response to receptor activation and its effect on the trans-Golgi network
    Sukru S Oner
    Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 288:24091-103. 2013
    ..We recently reported the regulation of the AGS3-Gαi signaling module by a cell surface, seven-transmembrane receptor...
  20. doi Cyclooxygenase-2 mediated regulation of E-cadherin occurs in conventional but not early-onset gastric cancer cell lines
    R Sitarz
    Department of Pathology, University Medical Centre, Utrecht, The Netherlands
    Cell Oncol 31:475-85. 2009
    ..A relationship between them is documented in non-small cell lung and prostate cancer. We present novel evidence of a relationship between COX-2 and E-cadherin expression in gastric cancer...
  21. pmc Nucleus accumbens AGS3 expression drives ethanol seeking through G betagamma
    M Scott Bowers
    Ernest Gallo Clinic and Research Center, Department of Neurology, University of California at San Francisco, Emeryville, CA 94608, USA
    Proc Natl Acad Sci U S A 105:12533-8. 2008
    ..A novel G protein modulator (Gpsm1/AGS3) was up-regulated in the rat nucleus accumbens core (NAcore) but not in other limbic nuclei during abstinence from ..
  22. pmc The Aspergillus fumigatus transcription factor Ace2 governs pigment production, conidiation and virulence
    Daniele E Ejzykowicz
    Los Angeles Biomedical Research Institute at Harbor UCLA Medical Center, Torrance, CA, USA
    Mol Microbiol 72:155-69. 2009
    ..The Deltaace2 mutant had reduced ppoC, ecm33 and ags3 mRNA expression. It is known that A...
  23. pmc Identification of an Aurora-A/PinsLINKER/Dlg spindle orientation pathway using induced cell polarity in S2 cells
    Christopher A Johnston
    Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA
    Cell 138:1150-63. 2009
    ..polarity in a normally unpolarized cell line and use this method to characterize Partner of Inscuteable (Pins; LGN/AGS3 in mammals) -dependent spindle orientation...
  24. doi Aicardi-Goutières syndrome: description of a late onset case
    Stefano D'Arrigo
    Department of Developmental Neurology, Fondazione IRCCS Istituto Neurologico C Besta, Milan, Italy
    Dev Med Child Neurol 50:631-4. 2008
    ..The syndrome can be caused by mutations in the AGS1 gene encoding the exonuclease TREX1, or in any of the AGS2, AGS3, or AGS4 genes that encode the three subunits of the human ribonuclease H2 (RNaseH2) complex...
  25. pmc Contributions of the two accessory subunits, RNASEH2B and RNASEH2C, to the activity and properties of the human RNase H2 complex
    Hyongi Chon
    Program in Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda MD 20892, USA
    Nucleic Acids Res 37:96-110. 2009
    ..In contrast, the RNASEH2B and RNASEH2C subunits from human and Saccharomyces cerevisiae share very little homology, although they both form soluble B/C ..
  26. pmc Up-regulation of AGS3 during morphine withdrawal promotes cAMP superactivation via adenylyl cyclase 5 and 7 in rat nucleus accumbens/striatal neurons
    Peidong Fan
    Gilead Sciences, Inc, 3172 Porter Drive, Palo Alto, California 94304, USA
    Mol Pharmacol 76:526-33. 2009
    ..Recent evidence implicates an activator of G-protein signaling 3 (AGS3) in opiate addiction...
  27. ncbi Structural and infrared spectroscopic studies of some novel mechanochemically accessed adducts of silver(I) oxoanion salts with thiourea
    Graham A Bowmaker
    Department of Chemistry, University of Auckland, Private Bag 92019, Auckland, New Zealand
    Inorg Chem 48:3185-97. 2009
    ..In the 1:3 nitrate complex, the one independent silver atom major component (0.78(1)) is closely trigonal planar AgS3 (Ag-S 2.518(2)-2.592(1) A, Sigma(S-Ag-S) 359.7(0) degrees); there are minor nearby components (0...
  28. pmc An inhibitory role of the G-protein regulator AGS3 in mTOR-dependent macroautophagy
    Benjamin Groves
    Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara, Santa Barbara, California, United States of America
    PLoS ONE 5:e8877. 2010
    ..Although a previous report that AGS3 over-expression promotes macroautophagy suggests a stimulatory role of AGS3 in this process, we have found that ..
  29. pmc A structural determinant that renders G alpha(i) sensitive to activation by GIV/girdin is required to promote cell migration
    Mikel Garcia-Marcos
    Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 285:12765-77. 2010
    ..e. G betagamma, AGS3 (a guanine nucleotide dissociation inhibitor), GAIP/RGS19 (a GTPase-activating protein), and LPAR1 (a G protein-..
  30. pmc Amphetamine up-regulates activator of G-protein signaling 1 mRNA and protein levels in rat frontal cortex: the role of dopamine and glucocorticoid receptors
    M Schwendt
    Department of Neurosciences, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Neuroscience 168:96-107. 2010
    ..A single exposure to AMPH (2.5 mg/kg i.p.) selectively induced gene expression of AGS1, but not Rhes or AGS3 proteins, in the rat prefrontal cortex (PFC) as measured 3 h later...
  31. pmc Structural basis for interaction between the conserved cell polarity proteins Inscuteable and Leu-Gly-Asn repeat-enriched protein (LGN)
    Satoru Yuzawa
    Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, Fukuoka 812 8582, Japan
    Proc Natl Acad Sci U S A 108:19210-5. 2011
    ..residues that are critical for mInsc-LGN association, and reveal that the activator of G-protein signaling 3 (AGS3)-binding protein Frmpd1 [4...
  32. pmc α1,3 glucans are dispensable in Aspergillus fumigatus
    Christine Henry
    Unite des Aspergillus, Institut Pasteur, Paris, France
    Eukaryot Cell 11:26-9. 2012
    ..of Aspergillus fumigatus obtained by successive deletions of the three α1,3 glucan synthase genes (AGS1, AGS2, and AGS3) has a cell wall devoid of α1,3 glucans...
  33. pmc Dynamic localization of C. elegans TPR-GoLoco proteins mediates mitotic spindle orientation by extrinsic signaling
    Adam D Werts
    Biology Department, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Development 138:4411-22. 2011
    ..Proteins containing TPR and GoLoco-domains (C. elegans GPR-1/2, Drosophila Pins, vertebrate LGN and AGS3) are candidates for mediating mitotic spindle orientation by extrinsic signals, but the mechanisms by which TPR-..
  34. pmc Loss of activator of G-protein signaling 3 impairs renal tubular regeneration following acute kidney injury in rodents
    Kevin R Regner
    Division of Nephrology, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    FASEB J 25:1844-55. 2011
    ..In this report, we demonstrate that activator of G-protein signaling 3 (AGS3), an unconventional receptor-independent regulator of heterotrimeric G-protein function, influences renal tubular ..
  35. pmc Asymmetric cell divisions promote Notch-dependent epidermal differentiation
    Scott E Williams
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, New York 10065, USA
    Nature 470:353-8. 2011
    ..asymmetrically distributed at the cell cortex during mitosis: Par3-Par6-aPKC, which confer polarity, and Gα(i)-LGN/AGS3-NuMA-dynein/dynactin, which govern spindle positioning...
  36. pmc A GDI (AGS3) and a GEF (GIV) regulate autophagy by balancing G protein activity and growth factor signals
    Mikel Garcia-Marcos
    Departments of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 22:673-86. 2011
    ..a key role in regulating autophagy and that dynamic interplay between Gα(i3), activator of G-protein signaling 3 (AGS3, its guanine nucleotide dissociation inhibitor), and GIV determines whether autophagy is promoted or inhibited...
  37. pmc The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease
    Martin A M Reijns
    Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh EH4 2XU, United Kingdom
    J Biol Chem 286:10530-9. 2011
    ..This C-terminal extension interacts with the RNASEH2C C terminus and both are necessary to form a stable, enzymatically active heterotrimer...
  38. pmc Activators of G-protein signaling 3: a drug addiction molecular gateway
    Michael Scott Bowers
    Department of Psychiatry, Virginia Commonwealth University School of Medicine, Richmond, VA 23298, USA
    Behav Pharmacol 21:500-13. 2010
    ..data showing that protein expression and signaling through the nonreceptor activator of G-protein signaling 3 (AGS3) are altered by commonly abused substances in rat and in in-vitro addiction models...
  39. pmc Activator of G protein signaling 3 promotes epithelial cell proliferation in PKD
    Rama Nadella
    Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Am Soc Nephrol 21:1275-80. 2010
    ..In this study, we report abnormal overexpression of activator of G protein signaling 3 (AGS3), a receptor-independent regulator of heterotrimeric G proteins, in rodents and humans with both autosomal ..
  40. pmc Identification of a deubiquitinating enzyme as a novel AGS3-interacting protein
    Zhuojin Xu
    Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara, Santa Barbara, California, United States of America
    PLoS ONE 5:e9725. 2010
    Activator of G protein Signaling 3 (AGS3) is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/..
  41. pmc Regulation of the AGS3·G{alpha}i signaling complex by a seven-transmembrane span receptor
    Sukru Sadik Oner
    Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 285:33949-58. 2010
    ..b>AGS3 (GPSM1) contains four G-protein regulatory motifs (GPR) that directly bind Gα(i) free of Gβγ providing an ..
  42. ncbi [The effect of AGS3 on the I(A) of newborn rat prefrontal cortical neurons pretreated by chronic morphine]
    Ying Zhao
    Department of Physiology, Luzhou Medical College, Luzhou 646000, China
    Zhongguo Ying Yong Sheng Li Xue Za Zhi 26:191-4. 2010
    ..On this basis, we use AGS3 antibody to inhibit the function of AGS3, for observing the impact of AGS3 on the I(A), thus further explore the ..
  43. pmc Probing neurochemical structure and function of retinal ON bipolar cells with a transgenic mouse
    Anuradha Dhingra
    Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6058, USA
    J Comp Neurol 510:484-96. 2008
    ..The following transcripts were not found in our library: AGS3 (GPSM1), RGS10, RGS19 (GAIP), calbindin, GC1alpha2, GC1beta2, PDE5, PDE2A, amiloride-sensitive sodium channel ACCN4,..
  44. ncbi Thermodynamic characterization of the binding of activator of G protein signaling 3 (AGS3) and peptides derived from AGS3 with G alpha i1
    Anirban Adhikari
    Department of Biochemistry and Molecular Biophysics Graduate Program, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 278:51825-32. 2003
    Activator of G protein signaling 3 (AGS3) is a guanine nucleotide dissociation inhibitor (GDI) that contains four G protein regulatory (GPR) or GoLoco motifs in its C-terminal domain...
  45. pmc Subcellular localization of LGN during mitosis: evidence for its cortical localization in mitotic cell culture systems and its requirement for normal cell cycle progression
    Rachna Kaushik
    Institute of Molecular and Cell Biology, Singapore 117609
    Mol Biol Cell 14:3144-55. 2003
    Mammalian LGN/AGS3 proteins and their Drosophila Pins orthologue are cytoplasmic regulators of G-protein signaling...
  46. ncbi Receptor- and nucleotide exchange-independent mechanisms for promoting G protein subunit dissociation
    Mousumi Ghosh
    Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 278:34747-50. 2003
    ..b>AGS3, a member of the activators of G protein signaling family of proteins, also activates G protein signaling in a ..
  47. ncbi Influence of cytosolic AGS3 on receptor--G protein coupling
    Hongzheng Ma
    Department of Biochemistry and Molecular Pharmacology, West Virginia University School of Medicine, Morgantown, West Virginia 26506 9142, USA
    Biochemistry 42:8085-93. 2003
    Activator of G protein signaling 3 (AGS3) activates the Gbetagamma mating pathway in yeast in a manner that is independent of heptahelical receptors...
  48. ncbi Asymmetrically distributed C. elegans homologs of AGS3/PINS control spindle position in the early embryo
    Monica Gotta
    Wellcome Cancer Research United Kingdom Institute, Tennis Court Road, University of Cambridge, United Kingdom
    Curr Biol 13:1029-37. 2003
    ..The Galpha subunits act downstream of the PAR polarity proteins, which control the relative pulling forces acting on the poles. How these heterotrimeric G proteins are regulated and how they control spindle position is still unknown...
  49. ncbi Interaction of activator of G-protein signaling 3 (AGS3) with LKB1, a serine/threonine kinase involved in cell polarity and cell cycle progression: phosphorylation of the G-protein regulatory (GPR) motif as a regulatory mechanism for the interaction of GP
    Joe B Blumer
    Chugai Pharmaceutical Co, Ltd, Tsukuba Research Laboratory, 153 2 Nagai Niihari, Ibaraki 300 4101, Japan
    J Biol Chem 278:23217-20. 2003
    Activator of G-protein signaling 3 (AGS3) has a modular domain structure consisting of seven tetratricopeptide repeats (TPRs) and four G-protein regulatory (GPR) motifs...
  50. ncbi The G-protein regulator AGS3 controls an early event during macroautophagy in human intestinal HT-29 cells
    Sophie Pattingre
    INSERM U504, 16 Avenue Paul Vaillant Couturier, 94807 Villejuif Cedex, France
    J Biol Chem 278:20995-1002. 2003
    b>AGS3 contains GoLoco or G-protein regulatory motifs in its COOH-terminal half that stabilize the GDP-bound conformation of the alpha-subunit of the trimeric Gi3 protein...
  51. ncbi Expression analysis and subcellular distribution of the two G-protein regulators AGS3 and LGN indicate distinct functionality. Localization of LGN to the midbody during cytokinesis
    Joe B Blumer
    Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
    J Biol Chem 277:15897-903. 2002
    Activator of G-protein signaling 3 (AGS3) and LGN have a similar domain structure and contain four G-protein regulatory motifs that serve as anchors for the binding of the GDP-bound conformation of specific G-protein alpha subunits...
  52. ncbi Identification of a truncated form of the G-protein regulator AGS3 in heart that lacks the tetratricopeptide repeat domains
    N Pizzinat
    Department of Pharmacology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 276:16601-10. 2001
    b>AGS3, a 650-amino acid protein encoded by an approximately 4-kilobase (kb) mRNA enriched in rat brain, is a Galpha(i)/Galpha(t)-binding protein that competes with Gbetagamma for interaction with Galpha(GDP) and acts as a guanine ..
  53. ncbi Selective interaction of AGS3 with G-proteins and the influence of AGS3 on the activation state of G-proteins
    M L Bernard
    Department of Pharmacology, Medical University of South Carolina, Charleston, South Carolina 29403, USA
    J Biol Chem 276:1585-93. 2001
    b>AGS3 (activator of G-protein signaling 3) was isolated in a yeast-based functional screen for receptor-independent activators of heterotrimeric G-proteins...
  54. ncbi AGS3 inhibits GDP dissociation from galpha subunits of the Gi family and rhodopsin-dependent activation of transducin
    M Natochin
    Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 275:40981-5. 2000
    ..Activator of G protein signaling 3 (AGS3), identified as an activator of the yeast pheromone pathway in the absence of the pheromone receptor, has a domain ..
  55. ncbi Stabilization of the GDP-bound conformation of Gialpha by a peptide derived from the G-protein regulatory motif of AGS3
    Y K Peterson
    Departments of Pharmacology and Library and Informatics, Medical University of South Carolina, Charleston, South Carolina 29403, USA
    J Biol Chem 275:33193-6. 2000
    The G-protein regulatory (GPR) motif in AGS3 was recently identified as a region for protein binding to heterotrimeric G-protein alpha subunits...
  56. ncbi Receptor-independent activators of heterotrimeric G-protein signaling pathways
    A Takesono
    Department of Pharmacology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 274:33202-5. 1999
    ..We identified two mammalian proteins, AGS2 and AGS3 (activators of G-protein signaling), that activated the pheromone response pathway at the level of heterotrimeric G-..
  57. pmc A small protein (Ags1p) and the Pho80p-Pho85p kinase complex contribute to aminoglycoside antibiotic resistance of the yeast Saccharomyces cerevisiae
    S Wickert
    Institut fur Mikrobiologie, Heinrich Heine Universitat, Dusseldorf, Germany
    J Bacteriol 180:1887-94. 1998
    We identified the AGS1 and AGS3 genes by their ability to partially complement an ags mutant (RC1707) which is supersensitive to various aminoglycoside antibiotics (J. F. Ernst and R. K. Chan, J. Bacteriol. 163:8-14, 1985)...
  58. ncbi Glutamate transmission and addiction to cocaine
    Peter W Kalivas
    Department of Physiology and Neuroscience, Medical University of South Carolina, Charleston, South Carolina 29464, USA
    Ann N Y Acad Sci 1003:169-75. 2003
    ..1) Neurons in the prefrontal cortex have increased levels of activator of G protein signaling 3 (AGS3) that causes reduced signaling through Gi coupled receptors, and normalization of AGS3 blocked cocaine-primed ..
  59. ncbi AGS3 and signal integration by Galpha(s)- and Galpha(i)-coupled receptors: AGS3 blocks the sensitization of adenylyl cyclase following prolonged stimulation of a Galpha(i)-coupled receptor by influencing processing of Galpha(i)
    Motohiko Sato
    Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
    J Biol Chem 279:13375-82. 2004
    b>AGS3-LONG and AGS3-SHORT contain G-protein regulatory motifs that interact with and stabilize the GDP-bound conformation of Galpha(i) > Galpha(o)...
  60. pmc The PDZ and band 4.1 containing protein Frmpd1 regulates the subcellular location of activator of G-protein signaling 3 and its interaction with G-proteins
    Ningfei An
    Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
    J Biol Chem 283:24718-28. 2008
    Activator of G-protein signaling 3 (AGS3) is one of nine mammalian proteins containing one or more G-protein regulatory (GPR) motifs that stabilize the GDP-bound conformation of Galphai...
  61. pmc Ric-8A catalyzes guanine nucleotide exchange on G alphai1 bound to the GPR/GoLoco exchange inhibitor AGS3
    Celestine J Thomas
    Division of Biological Science, University of Montana, Missoula, Montana 59812, USA
    J Biol Chem 283:23150-60. 2008
    ..A host of proteins, including Galpha subunits, Ric-8, AGS3, regulators of G-protein signalings, and scaffolding proteins, coordinate this vital cellular process...
  62. pmc An update on adenosine A2A-dopamine D2 receptor interactions: implications for the function of G protein-coupled receptors
    S Ferre
    National Institute on Drug Abuse, IRP, NIH, DHHS, 5500 Nathan Shock Dr, Baltimore, MD 21224, USA
    Curr Pharm Des 14:1468-74. 2008
    ..Finally, under conditions of upregulation of an activator of G protein signaling (AGS3), such as during chronic treatment with addictive drugs, a synergistic A(2A)-D(2) receptor interaction can also be ..
  63. pmc Activator of G protein signaling 3 null mice: I. Unexpected alterations in metabolic and cardiovascular function
    Joe B Blumer
    Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Endocrinology 149:3842-9. 2008
    ..As part of a broad effort to define the extent of functional diversity of AGS3-regulated-events in vivo, we generated AGS3 null mice...
  64. pmc A specific role of AGS3 in the surface expression of plasma membrane proteins
    B Groves
    Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106
    Proc Natl Acad Sci U S A 104:18103-8. 2007
    Activator of G protein signaling 3 (AGS3), originally identified in a functional screen for mammalian proteins that activate heterotrimeric G protein signaling, is known to be involved in drug-seeking behavior and is up-regulated during ..
  65. ncbi AGS3, an alpha(1-3)glucan synthase gene family member of Aspergillus fumigatus, modulates mycelium growth in the lung of experimentally infected mice
    D Maubon
    Aspergillus Unit, Institut Pasteur, Paris, France
    Fungal Genet Biol 43:366-75. 2006
    ..In A. fumigatus, three putative alpha(1-3)glucan synthase genes AGS1, AGS2 and AGS3 have been identified...
  66. ncbi Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3
    Tomoko Izaki
    Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 341:1001-6. 2006
    ..Nevertheless, human Insc proteins bind to the human Pins homologues LGN and AGS3, and also to human Par3 and its related protein Par3beta...
  67. ncbi Activator of G protein signaling type 3 mRNA is widely distributed in the rat brain and is particularly abundant in the subventricular zone-olfactory bulb system of neural precursor cell proliferation, migration and differentiation
    Jean Marc Taymans
    Central Nervous System Discovery Research, Johnson and Johnson Pharmaceutical Research and Development, 2340 Beerse, Belgium
    Neurosci Lett 391:116-21. 2006
    Activator of G protein signaling 3 (AGS3) is a guanine nucleotide dissociation inhibitor (GDI) to heterotrimeric G proteins of the Galphai/o class...
  68. ncbi G protein betagamma subunits and AGS3 control spindle orientation and asymmetric cell fate of cerebral cortical progenitors
    Kamon Sanada
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Cell 122:119-31. 2005
    ..Silencing AGS3, a nonreceptor activator of Gbetagamma, results in defects similar to the impairment of Gbetagamma, providing ..
  69. pmc Activator of G protein signaling 3 regulates opiate activation of protein kinase A signaling and relapse of heroin-seeking behavior
    Lina Yao
    Ernest Gallo Clinic and Research Center, 5858 Horton Street, Suite 200, Emeryville, CA 94608, USA
    Proc Natl Acad Sci U S A 102:8746-51. 2005
    ..Activation requires Galpha(i3) and an activator of G protein signaling 3 (AGS3). AGS3 competes with betagamma for binding to Galpha(i3)-GDP and enhances the action of unbound betagamma...
  70. ncbi Interaction of transducin-alpha with LGN, a G-protein modulator expressed in photoreceptor cells
    Vasiliy S Kerov
    Department of Physiology and Biophysics, University of Iowa College of Medicine, BSB 5 532, Iowa City, IA 52242 1109, USA
    Mol Cell Neurosci 28:485-95. 2005
    LGN and activator of G-protein signaling 3 (AGS3) belong to the class of G-protein modulators containing G-protein regulatory motifs (GPR proteins). Evidence for the functions of these molecules has only started to emerge...
  71. ncbi AGS proteins, GPR motifs and the signals processed by heterotrimeric G proteins
    Stephen M Lanier
    Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, 1901 Perdido St New Orleans, LA 70112, USA
    Biol Cell 96:369-72. 2004
    ..We identified three proteins, AGS1, AGS2 and AGS3 (for Activators of G-protein Signaling), that activated heterotrimeric G-protein signalling pathways in the absence ..
  72. ncbi Return of the GDI: the GoLoco motif in cell division
    Francis S Willard
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, and UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599 7365, USA
    Annu Rev Biochem 73:925-51. 2004
    ..signaling regulators, such as Loco, RGS12, RGS14, and Rap1GAP, as well as in tandem arrays in proteins, such as AGS3, G18, LGN, Pcp-2/L7, and Partner of Inscuteable (Pins/Rapsynoid)...
  73. pmc Activator of G protein signaling 3: a gatekeeper of cocaine sensitization and drug seeking
    M Scott Bowers
    Department of Physiology and Neuroscience, Medical University of South Carolina, Charleston, SC 29425, USA
    Neuron 42:269-81. 2004
    Chronic cocaine administration reduces G protein signaling efficacy. Here, we report that the expression of AGS3, which binds to GialphaGDP and inhibits GDP dissociation, was upregulated in the prefrontal cortex (PFC) during late ..
  74. ncbi Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells
    Tannin J Fuja
    Developmental Biology Center, University of California, Irvine, California 92697, USA
    J Neurosci Res 75:782-93. 2004
    ..We show by indirect immunofluorescence, confocal microscopy, and time-lapse video-microscopy that LGN and AGS3, two human homologs of the Drosophila ACD determinant Pins, have distinct patterns of localization in hNPCs...
  75. ncbi Evidence that the SRY protein is encoded by a single exon on the human Y chromosome
    M A Behlke
    Howard Hughes Research Laboratories, Whitehead Institute, Cambridge, Massachusetts
    Genomics 17:736-9. 1993
    ..Within this CpG island lies the sequence CGCCCCGC, a potential binding site for the EGR-1/WT1 family of transcription factors, some of which appear to function in gonadal development...

Research Grants56

  1. Mechanisms of Mitotic Spindle Orientation in Mammalian Cells
    Quansheng Du; Fiscal Year: 2009
    ..Recent studies have revealed the central role played by Pins (LGN and AGS3 in mammals) and the heterotrimeric G protein alpha subunit (Ga) in regulating spindle orientation during asymmetric ..
  2. Regulation of Chemokine Mediated Leukocyte Functions
    Micheler Ricardo Richardson; Fiscal Year: 2010
    ..studies have identified the G protein-coupled receptor kinase 6 (GRK6), and the activator of G protein signaling 3 (AGS3) as important regulators of CXCR2-mediated leukocyte functions...
  3. Ethanol regulation of signaling: from cells to behavior
    Ivan Diamond; Fiscal Year: 2009
    ..ethanol and NPA for activation of PKA signaling; Il: Test whether a specific activator of G protein signaling, AGS3, selectively regulates synergy between D2 and ethanol; III: Test which specific beta/gamma dimers released upon D2 ..
  4. Mechanisms of Mitotic Spindle Orientation in Mammalian Cells
    Quansheng Du; Fiscal Year: 2007
    ..Recent studies have revealed the central role played by Pins (LGN and AGS3 in mammals) and the heterotrimeric G protein alpha subunit (Ga) in regulating spindle orientation during asymmetric ..
  5. Mechanisms of Mitotic Spindle Orientation in Mammalian Cells
    Quansheng Du; Fiscal Year: 2010
    ..Recent studies have revealed the central role played by Pins (LGN and AGS3 in mammals) and the heterotrimeric G protein alpha subunit (Ga) in regulating spindle orientation during asymmetric ..
  6. Defining the role of AGS3 in G protein signal processing
    JOE BLUMER; Fiscal Year: 2004
    ..This application focuses on one such accessory protein, Activator of G protein Signaling 3 (AGS3), which was identified in a functional screen for receptor-independent activation of G proteins...
  7. Accessory proteins and G-protein signaling mechanisms
    STEPHEN LANIER; Fiscal Year: 2006
    ..This proposal focuses on AGS3 as a template for understanding the role of such proteins in signal processing...
  8. Accessory proteins and G-protein signaling mechanisms
    STEPHEN LANIER; Fiscal Year: 2007
    ..This proposal focuses on AGS3 as a template for understanding the role of such proteins in signal processing...
  9. Accessory proteins and G-protein signaling mechanisms
    STEPHEN LANIER; Fiscal Year: 2003
    ..This proposal focuses on AGS3 as a template for understanding the role of such proteins in signal processing...
  10. G-Protein Regulation of Ethanol Action
    Michael Bowers; Fiscal Year: 2007
    ..This proposal focuses on the Activator of G-protein Signaling (AGS3). AGS3 expression increases during withdrawal from repeated cocaine administration...
  11. Accessory proteins and G-protein signaling mechanisms
    Stephen M Lanier; Fiscal Year: 2010
    ..This proposal focuses on AGS3 and related Group II AGS proteins...
  12. AGS4 in G-protein Signal Processing
    Joe B Blumer; Fiscal Year: 2010
    ..Group II AGS proteins include AGS3 and AGS5/LGN, which play important regulatory roles in cell division, neuronal development, synaptic plasticity, ..
  13. Evaluating models of cocaine self-administration
    Lori Knackstedt; Fiscal Year: 2007
    ..changes that accompany cocaine self-administration, namely in glutamate transmission-related proteins such as AGS3, PSD-95, mGluR1/5, xCT, GluR1, GluR2/3, and the Homer proteins...
  14. Neurobiology of Addiction Research Center (NARC)
    Peter Kalivas; Fiscal Year: 2007
    ..The NARC also takes advantage of the rapid growth in basic neuroscience at MUSC, as well as the existing clinical strengths in addiction research to provide a means for actively mentoring faculty and trainees. ..
  15. COCAINE, OPIOIDS AND DRUG ABUSE
    Peter Kalivas; Fiscal Year: 2007
    ..abstract_text> ..
  16. GLUTAMATE AND CRAVING FOR COCAINE
    Peter Kalivas; Fiscal Year: 2009
    ..The overarching goal of the proposal is to identify novel molecular targets for pharmacotherapeutic intervention in cocaine craving and relapse. ..
  17. GLUTAMATE AND CRAVING FOR COCAINE
    Peter W Kalivas; Fiscal Year: 2010
    ....
  18. Cocaine, Opoids and Drug Abuse
    Peter W Kalivas; Fiscal Year: 2010
    ..Accordingly, we propose to characterize the ability of daily N-acetylcysteine treatment to produce enduring protection from the reinstatement of cocaine seeking, and associate this protection with restoring neuroplasticity. ..
  19. Stress-induced reinstatement of cocaine-seeking behavior
    Peter Kalivas; Fiscal Year: 2007
    ..abstract_text> ..
  20. SPECTRAL CONFOCAL MICROSCOPE
    Peter Bryant; Fiscal Year: 2002
    ..abstract_text> ..
  21. REGULATION OF MESOLIMBIC DOPAMINE BY ENDOGENOUS COMPOUND
    Peter Kalivas; Fiscal Year: 1999
    ....
  22. Neuronal Transplants as an Organotypic Brain Tumor Model
    Peter Bryant; Fiscal Year: 2002
    ..Eventually it may be possible to use it to explore the effectiveness of therapeutic strategies including gene therapy and drug treatments. ..
  23. Molecular Genetics of Drosophila MAGUK's
    Peter Bryant; Fiscal Year: 2005
    ..The work will provide a genetic approach to understanding the functions of Dlg and its mammalian homologs, some of which are strongly implicated in a variety of human cancers. ..
  24. GLUTAMATE AND CRAVING FOR COCAINE
    Peter Kalivas; Fiscal Year: 2004
    ..In addition, microdialysis will be employed to estimate changes in glutamate transmission in the nucleus accumbens following cocaine or cue-induced reinstatement. ..
  25. REGULATION OF MESOLIMBIC DOPAMINE BY ENDOGENOUS COMPOUND
    Peter Kalivas; Fiscal Year: 2003
    ..Furthermore, the anatomical and functional mapping of the motive circuit will provide a basis for interpreting future imaging studies in humans where attempts are made to correlate changes in spatially discrete nuclei. ..