RILP

Summary

Gene Symbol: RILP
Description: Rab interacting lysosomal protein
Alias: PP10141, rab-interacting lysosomal protein
Species: human
Products:     RILP

Top Publications

  1. Wang T, Wong K, Hong W. A unique region of RILP distinguishes it from its related proteins in its regulation of lysosomal morphology and interaction with Rab7 and Rab34. Mol Biol Cell. 2004;15:815-26 pubmed
    ..and Rab34 are implicated in regulation of lysosomal morphology and they share a common effector referred to as the RILP (Rab-interacting lysosomal protein)...
  2. Wang T, Hong W. RILP interacts with VPS22 and VPS36 of ESCRT-II and regulates their membrane recruitment. Biochem Biophys Res Commun. 2006;350:413-23 pubmed
    b>RILP is emerging as a key regulator of late endocytic pathway by functioning as a downstream effector of activated Rab7 and Rab34, while ESCRT-I-->ESCRT-II-->ESCRT-III machinery acts in sorting proteins to the multivesicular body (..
  3. Caplan S, Hartnell L, Aguilar R, Naslavsky N, Bonifacino J. Human Vam6p promotes lysosome clustering and fusion in vivo. J Cell Biol. 2001;154:109-22 pubmed
    ..This study implicates hVam6p as a mammalian tethering/docking factor characterized with intrinsic ability to promote lysosome fusion in vivo. ..
  4. Cantalupo G, Alifano P, Roberti V, Bruni C, Bucci C. Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes. EMBO J. 2001;20:683-93 pubmed
    ..We named it RILP (Rab-interacting lysosomal protein) since it can be recruited efficiently on late endosomal and lysosomal membranes ..
  5. Jordens I, Fernandez Borja M, Marsman M, Dusseljee S, Janssen L, Calafat J, et al. The Rab7 effector protein RILP controls lysosomal transport by inducing the recruitment of dynein-dynactin motors. Curr Biol. 2001;11:1680-5 pubmed
    ..We and others [7] have identified a protein, called RILP (for Rab7-interacting lysosomal protein), that interacts with active Rab7 on late endosomes and lysosomes...
  6. Wang T, Hong W. Interorganellar regulation of lysosome positioning by the Golgi apparatus through Rab34 interaction with Rab-interacting lysosomal protein. Mol Biol Cell. 2002;13:4317-32 pubmed
    ..with the membrane mediated by prenylation and its direct interaction with Rab-interacting lysosomal protein (RILP). This biological activity, mediated by Rab34-RILP interaction, is dependent on Lys82 in the switch I region...
  7. Progida C, Malerød L, Stuffers S, Brech A, Bucci C, Stenmark H. RILP is required for the proper morphology and function of late endosomes. J Cell Sci. 2007;120:3729-37 pubmed
    ..The Rab7-interacting lysosomal protein RILP is interesting in this context as it interacts with Vps22 (also known as EAP30) and Vps36 (also known as EAP45), ..
  8. Wu M, Wang T, Loh E, Hong W, Song H. Structural basis for recruitment of RILP by small GTPase Rab7. EMBO J. 2005;24:1491-501 pubmed
    ..The crystal structure of Rab7-GTP in complex with the Rab7 binding domain of RILP reveals that Rab7 interacts with RILP specifically via two distinct areas, with the first one involving the switch ..
  9. Sapmaz A, Berlin I, Bos E, Wijdeven R, Janssen H, Konietzny R, et al. USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7. Nat Commun. 2019;10:1454 pubmed publisher
    ..Mechanistic studies reveal that LE transport effector RILP prefers ubiquitylation-deficient Rab7, while retromer-mediated LE recycling benefits from an intact cycle of Rab7 ..

More Information

Publications55

  1. Baba T, Tóth D, Sengupta N, Kim Y, Balla T. Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKMH1 membrane cycling during autophagosome-lysosome fusion. EMBO J. 2019;38: pubmed publisher
    ..Rab7 effectors Vps35 and RILP are not affected by acute PI(4,5)P2 production...
  2. Ma X, Liu K, Li J, Li H, Li J, Liu Y, et al. A non-canonical GTPase interaction enables ORP1L-Rab7-RILP complex formation and late endosome positioning. J Biol Chem. 2018;293:14155-14164 pubmed publisher
    ..several large protein complexes, one of which contains the small GTPase Rab7, Rab-interacting lysosomal protein (RILP), and oxysterol-binding protein-related protein 1 (ORP1L)...
  3. Daniele T, Hackmann Y, Ritter A, Wenham M, Booth S, Bossi G, et al. A role for Rab7 in the movement of secretory granules in cytotoxic T lymphocytes. Traffic. 2011;12:902-11 pubmed publisher
    ..GTP-bound Rab7 and Rab interacting lysosomal protein, RILP, interact and both localize to secretory lysosomes in CTL...
  4. van Luijn M, Kreft K, Jongsma M, Mes S, Wierenga Wolf A, van Meurs M, et al. Multiple sclerosis-associated CLEC16A controls HLA class II expression via late endosome biogenesis. Brain. 2015;138:1531-47 pubmed publisher
    ..performing co-immunoprecipitations, we found that CLEC16A directly binds to two critical members of this complex, RILP and the HOPS complex...
  5. Rosa Ferreira C, Sweeney S, Munro S. The small G protein Arl8 contributes to lysosomal function and long-range axonal transport in Drosophila. Biol Open. 2018;7: pubmed publisher
    ..revealed binding of Drosophila Arl8 to the HOPS complex, and to the Drosophila ortholog of RILP, a protein that, in mammals, recruits dynein to late endosomes, with dynein being known to be required for neuronal ..
  6. Adams A, Weinman S, Wozniak A. Caspase-1 regulates cellular trafficking via cleavage of the Rab7 adaptor protein RILP. Biochem Biophys Res Commun. 2018;503:2619-2624 pubmed publisher
    ..Here we describe a mechanism by which cleavage of the Rab7 adaptor protein, RILP (Rab interacting lysosomal protein) is induced by viral infection...
  7. Johnson D, Ostrowski P, Jaumouillé V, Grinstein S. The position of lysosomes within the cell determines their luminal pH. J Cell Biol. 2016;212:677-92 pubmed publisher
    ..This in turn results in decreased recruitment of Rab-interacting lysosomal protein (RILP), an effector that regulates the recruitment and stability of the V1G1 component of the lysosomal V-ATPase...
  8. van der Kant R, Jonker C, Wijdeven R, Bakker J, Janssen L, Klumperman J, et al. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity. J Biol Chem. 2015;290:30280-90 pubmed publisher
    ..in HOPS has significantly changed to accommodate binding to mammalian-specific RAB7 interacting lysosomal protein (RILP)...
  9. D Costa V, Braun V, Landekic M, Shi R, Proteau A, McDonald L, et al. Salmonella Disrupts Host Endocytic Trafficking by SopD2-Mediated Inhibition of Rab7. Cell Rep. 2015;12:1508-18 pubmed publisher
    ..Consequently, this limits Rab7 interaction with its dynein- and kinesin-binding effectors RILP and FYCO1 and thereby disrupts host-driven regulation of microtubule motors...
  10. Margiotta A, Progida C, Bakke O, Bucci C. Rab7a regulates cell migration through Rac1 and vimentin. Biochim Biophys Acta Mol Cell Res. 2017;1864:367-381 pubmed publisher
    ..Indeed, Rab7a recruits on vesicles RILP and FYCO that are responsible for the movement of Rab7a-positive vesicles and/or organelles on microtubule tracks, ..
  11. Levin R, Hammond G, Balla T, De Camilli P, Fairn G, Grinstein S. Multiphasic dynamics of phosphatidylinositol 4-phosphate during phagocytosis. Mol Biol Cell. 2017;28:128-140 pubmed publisher
    ..Defective acidification may be caused by impaired recruitment of Rab7 effectors, including RILP, which were shown earlier to displace phagosomes toward perinuclear lysosomes...
  12. Modica G, Skorobogata O, Sauvageau E, Vissa A, Yip C, Kim P, et al. Rab7 palmitoylation is required for efficient endosome-to-TGN trafficking. J Cell Sci. 2017;130:2579-2590 pubmed publisher
    ..required for the degradation of EGFR or for its interaction with its effector, Rab-interacting lysosomal protein (RILP)...
  13. Lin X, Zhang J, Chen L, Chen Y, Xu X, Hong W, et al. Tyrosine phosphorylation of Rab7 by Src kinase. Cell Signal. 2017;35:84-94 pubmed publisher
    ..The tyrosine phosphorylation of Rab7 is physiologically induced by EGF, and impairs the interaction of Rab7 with RILP, consequently inhibiting EGFR degradation and sustaining Akt signaling...
  14. Amaya C, Militello R, Calligaris S, Colombo M. Rab24 interacts with the Rab7/Rab interacting lysosomal protein complex to regulate endosomal degradation. Traffic. 2016;17:1181-1196 pubmed publisher
    ..The Rab interacting lysosomal protein (RILP) is a downstream effector of Rab7 that recruits the functional dynein/dynactin motor complex ..
  15. Zhang J, Johnson J, He J, Napolitano G, Ramadass M, Rocca C, et al. Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A. J Biol Chem. 2017;292:10328-10346 pubmed publisher
    ..in cystinosis was found to associate with decreased expression of the small GTPase Rab11 and the Rab7 effector RILP. Defective Rab11 trafficking in cystinosis was rescued by treatment with small-molecule CMA activators...
  16. Li X, Rydzewski N, Hider A, Zhang X, Yang J, Wang W, et al. A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation. Nat Cell Biol. 2016;18:404-17 pubmed publisher
    ..LSDs, which is instead likely to be caused by secondary cholesterol accumulation that constitutively activates Rab7-RILP-dependent retrograde transport...
  17. Efergan A, Azouz N, Klein O, Noguchi K, Rothenberg M, Fukuda M, et al. Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex. J Immunol. 2016;196:1091-101 pubmed publisher
    ..We also show that this minus end transport of the SGs is mediated by the RILP-dynein complex and identify RILP as a novel effector of Rab12...
  18. Saridaki T, Nippold M, Dinter E, Roos A, Diederichs L, Fensky L, et al. FYCO1 mediates clearance of ?-synuclein aggregates through a Rab7-dependent mechanism. J Neurochem. 2018;146:474-492 pubmed publisher
    ..the Rab7 effectors FYVE and coiled-coil domain-containing protein 1 (FYCO1) and Rab-interacting lysosomal protein (RILP). FYCO1-EGFP-decorated vesicles containing ?-synuclein...
  19. Schaub J, Stearns T. The Rilp-like proteins Rilpl1 and Rilpl2 regulate ciliary membrane content. Mol Biol Cell. 2013;24:453-64 pubmed publisher
    ..These data suggest that Rilp-like proteins function in regulation of ciliary membrane protein concentration by promoting protein removal from ..
  20. Starling G, Yip Y, Sanger A, Morton P, Eden E, Dodding M. Folliculin directs the formation of a Rab34-RILP complex to control the nutrient-dependent dynamic distribution of lysosomes. EMBO Rep. 2016;17:823-41 pubmed publisher
    ..FLCN interacts directly via its C-terminal DENN domain with the Rab34 effector RILP Using purified recombinant proteins, we show that the FLCN-DENN domain does not act as a GEF for Rab34, but rather, ..
  21. Wijdeven R, Janssen H, Nahidiazar L, Janssen L, Jalink K, Berlin I, et al. Cholesterol and ORP1L-mediated ER contact sites control autophagosome transport and fusion with the endocytic pathway. Nat Commun. 2016;7:11808 pubmed publisher
    ..the ER protein VAP-A, forming ER-autophagosome contact sites, which prevent minus-end transport by the Rab7-RILP-dynein complex. ORP1L-mediated contact sites also inhibit localization of PLEKHM1 to Rab7...
  22. De Luca M, Cogli L, Progida C, Nisi V, Pascolutti R, Sigismund S, et al. RILP regulates vacuolar ATPase through interaction with the V1G1 subunit. J Cell Sci. 2015;128:2565 pubmed publisher
  23. De Luca M, Cogli L, Progida C, Nisi V, Pascolutti R, Sigismund S, et al. RILP regulates vacuolar ATPase through interaction with the V1G1 subunit. J Cell Sci. 2014;127:2697-708 pubmed publisher
    Rab-interacting lysosomal protein (RILP) is a downstream effector of the Rab7 GTPase...
  24. van der Kant R, Fish A, Janssen L, Janssen H, Krom S, Ho N, et al. Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L. J Cell Sci. 2013;126:3462-74 pubmed publisher
    ..that lysosomal tethering and transport are combined processes co-regulated by one multi-protein complex: RAB7-RILP-ORP1L...
  25. Liang C, Lee J, Inn K, Gack M, Li Q, Roberts E, et al. Beclin1-binding UVRAG targets the class C Vps complex to coordinate autophagosome maturation and endocytic trafficking. Nat Cell Biol. 2008;10:776-87 pubmed publisher
  26. Progida C, Spinosa M, De Luca A, Bucci C. RILP interacts with the VPS22 component of the ESCRT-II complex. Biochem Biophys Res Commun. 2006;347:1074-9 pubmed
    The Rab-interacting lysosomal protein (RILP) has been identified as an effector for the small GTPases Rab7 and Rab34. It has been demonstrated that Rab7 and RILP are key proteins for the biogenesis of lysosomes and phagolysosomes...
  27. Schroeder C, Ostrem J, Hertz N, Vale R. A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region. elife. 2014;3:e03351 pubmed publisher
    ..These studies provide the first structural information and insight into the evolutionary origin of the LIC as well as revealing how this critical subunit connects the dynein motor to cargo. ..
  28. Shimojo M. Huntingtin regulates RE1-silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF) nuclear trafficking indirectly through a complex with REST/NRSF-interacting LIM domain protein (RILP) and dynactin p150 Glued. J Biol Chem. 2008;283:34880-6 pubmed publisher
    ..The REST/NRSF-interacting LIM domain protein (RILP) has also been shown to regulate REST/NRSF nuclear translocation...
  29. Song P, Trajkovic K, Tsunemi T, Krainc D. Parkin Modulates Endosomal Organization and Function of the Endo-Lysosomal Pathway. J Neurosci. 2016;36:2425-37 pubmed publisher
  30. Colucci A, Campana M, Bellopede M, Bucci C. The Rab-interacting lysosomal protein, a Rab7 and Rab34 effector, is capable of self-interaction. Biochem Biophys Res Commun. 2005;334:128-33 pubmed
    Rab-interacting lysosomal protein (RILP) has been identified as an interacting partner of the small GTPases Rab7 and Rab34...
  31. Bucci C, De Gregorio L, Bruni C. Expression analysis and chromosomal assignment of PRA1 and RILP genes. Biochem Biophys Res Commun. 2001;286:815-9 pubmed
    PRA1 (prenylated Rab acceptor) is a general regulator of Rab proteins, while RILP (Rab interacting lysosomal protein) is a specific effector for Rab7. It has been shown that PRA1 interacts with Rab proteins and with VAMP2...
  32. López de Armentia M, Gauron M, Colombo M. Staphylococcus aureus Alpha-Toxin Induces the Formation of Dynamic Tubules Labeled with LC3 within Host Cells in a Rab7 and Rab1b-Dependent Manner. Front Cell Infect Microbiol. 2017;7:431 pubmed publisher
    ..of microtubules and the activity of the motor protein Kinesin-1 (Kif5B) and the Rab-interacting lysosomal protein (RILP). Our group has previously reported that ?-hemolysin, a secreted toxin of S...
  33. Shimojo M, Hersh L. Characterization of the REST/NRSF-interacting LIM domain protein (RILP): localization and interaction with REST/NRSF. J Neurochem. 2006;96:1130-8 pubmed
    ..factor (REST)/neuron-restrictive silencer factor (NRSF)-interacting Lin-11, Isl-1 and Mec-3 (LIM) domain protein (RILP) that we proposed functions in the nuclear translocation of the transcriptional repressor REST/NRSF...
  34. Uusi Rauva K, Kyttälä A, van der Kant R, Vesa J, Tanhuanpää K, Neefjes J, et al. Neuronal ceroid lipofuscinosis protein CLN3 interacts with motor proteins and modifies location of late endosomal compartments. Cell Mol Life Sci. 2012;69:2075-89 pubmed publisher
    ..directly with active, guanosine-5'-triphosphate (GTP)-bound Rab7 and with the Rab7-interacting lysosomal protein (RILP) that anchors the dynein motor...
  35. Rocha N, Kuijl C, van der Kant R, Janssen L, Houben D, Janssen H, et al. Cholesterol sensor ORP1L contacts the ER protein VAP to control Rab7-RILP-p150 Glued and late endosome positioning. J Cell Biol. 2009;185:1209-25 pubmed publisher
    ..to the dynactin subunit p150(Glued) bind to LEs via the Rab7 effector Rab7-interacting lysosomal protein (RILP) in association with the oxysterol-binding protein ORP1L...
  36. Lehmann M, Milev M, Abrahamyan L, Yao X, Pante N, Mouland A. Intracellular transport of human immunodeficiency virus type 1 genomic RNA and viral production are dependent on dynein motor function and late endosome positioning. J Biol Chem. 2009;284:14572-85 pubmed publisher
    ..Our results demonstrate that dynein motor function is important for regulating Gag and vRNA egress on endosomal membranes in the cytoplasm to directly impact on viral production. ..
  37. Jackson L, Nawabi P, Hentea C, Roark E, Haldar K. The Salmonella virulence protein SifA is a G protein antagonist. Proc Natl Acad Sci U S A. 2008;105:14141-6 pubmed publisher
    ..Importantly, binding to SKIP requires SifA residues W197 and E201 of the conserved WxxxE signature sequence, leading to the speculation that bacterial G protein mimicry may result in G protein antagonism. ..
  38. Harrison R, Bucci C, Vieira O, Schroer T, Grinstein S. Phagosomes fuse with late endosomes and/or lysosomes by extension of membrane protrusions along microtubules: role of Rab7 and RILP. Mol Cell Biol. 2003;23:6494-506 pubmed
    ..Active Rab7 on the phagosomal membrane associates with the effector protein RILP (Rab7-interacting lysosomal protein), which in turn bridges phagosomes with dynein-dynactin, a microtubule-..
  39. Wozniak A, Long A, Jones Jamtgaard K, Weinman S. Hepatitis C virus promotes virion secretion through cleavage of the Rab7 adaptor protein RILP. Proc Natl Acad Sci U S A. 2016;113:12484-12489 pubmed
    ..In both cases, viral infection causes cleavage of the Rab7 adaptor protein RILP (Rab interacting lysosomal protein), which is responsible for linking Rab7 vesicles to dynein motor complexes...
  40. Wang Z, Zhou Y, Hu X, Chen W, Lin X, Sun L, et al. RILP suppresses invasion of breast cancer cells by modulating the activity of RalA through interaction with RalGDS. Cell Death Dis. 2015;6:e1923 pubmed publisher
    b>RILP (Rab7-interacting lysosomal protein) is a key regulator for late endosomal/lysosomal trafficking, and probably a tumor suppressor in prostate cancer...
  41. Lin X, Yang T, Wang S, Wang Z, Yun Y, Sun L, et al. RILP interacts with HOPS complex via VPS41 subunit to regulate endocytic trafficking. Sci Rep. 2014;4:7282 pubmed publisher
    ..In this study, we report that RILP, a downstream effector of Rab7, interacts with HOPS complex and recruits HOPS subunits to the late endosomal ..
  42. Gomez Suaga P, Rivero Ríos P, Fdez E, Blanca Ramírez M, Ferrer I, Aiastui A, et al. LRRK2 delays degradative receptor trafficking by impeding late endosomal budding through decreasing Rab7 activity. Hum Mol Genet. 2014;23:6779-96 pubmed publisher
    ..Together, these findings provide novel insights into a previously unknown regulation of Rab7 activity by mutant LRRK2 which impairs membrane trafficking at very late stages of the endocytic pathway. ..
  43. Margiotta A, Progida C, Bakke O, Bucci C. Characterization of the role of RILP in cell migration. Eur J Histochem. 2017;61:2783 pubmed publisher
    Rab-interacting lysosomal protein (RILP) is a regulator of late stages of endocytosis...
  44. Spinosa M, Progida C, De Luca A, Colucci A, Alifano P, Bucci C. Functional characterization of Rab7 mutant proteins associated with Charcot-Marie-Tooth type 2B disease. J Neurosci. 2008;28:1640-8 pubmed publisher
    ..The CMT2B-associated Rab7 proteins were also able to bind the Rab7 effector RILP (Rab-interacting lysosomal protein) and to rescue Rab7 function after silencing...
  45. Cardoso C, Leventer R, Ward H, Toyo oka K, Chung J, Gross A, et al. Refinement of a 400-kb critical region allows genotypic differentiation between isolated lissencephaly, Miller-Dieker syndrome, and other phenotypes secondary to deletions of 17p13.3. Am J Hum Genet. 2003;72:918-30 pubmed
  46. Marsman M, Jordens I, Rocha N, Kuijl C, Janssen L, Neefjes J. A splice variant of RILP induces lysosomal clustering independent of dynein recruitment. Biochem Biophys Res Commun. 2006;344:747-56 pubmed
    ..A critical mediator is the Rab7 effector RILP that recruits the minus-end dynein-dynactin motor complex to these compartments...