Gene Symbol: RIF1
Description: replication timing regulatory factor 1
Alias: telomere-associated protein RIF1, RAP1 interacting factor homolog, rap1-interacting factor 1 homolog
Species: human
Products:     RIF1

Top Publications

  1. Silverman J, Takai H, Buonomo S, Eisenhaber F, de Lange T. Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. Genes Dev. 2004;18:2108-19 pubmed
    We report on the function of the human ortholog of Saccharomyces cerevisiae Rif1 (Rap1-interacting factor 1). Yeast Rif1 associates with telomeres and regulates their length...
  2. Yamazaki S, Ishii A, Kanoh Y, Oda M, Nishito Y, Masai H. Rif1 regulates the replication timing domains on the human genome. EMBO J. 2012;31:3667-77 pubmed publisher
    ..However, how the genome-wide replication timing domains are established is unknown. Here, we show that Rif1 (Rap1-interacting-factor-1), originally identified as a telomere-binding factor in yeast, is a critical determinant ..
  3. Barazas M, Gasparini A, Huang Y, Küçükosmanoglu A, Annunziato S, Bouwman P, et al. Radiosensitivity Is an Acquired Vulnerability of PARPi-Resistant BRCA1-Deficient Tumors. Cancer Res. 2019;79:452-460 pubmed publisher
    ..acquire drug resistance through restoration of HR activity by the loss of end-resection antagonists of the 53BP1/RIF1/REV7/Shieldin/CST pathway...
  4. Lustig A. Towards the Mechanism of Yeast Telomere Dynamics. Trends Cell Biol. 2019;29:361-370 pubmed publisher
    ..Recent molecular and genetic studies of the yeast telosomal components Rap1, Rif1, and Rif2, the Mre11 complex, and Tel1ATM promise to increase our insight into the coordination between ..
  5. Díaz Gay M, Franch Expósito S, Arnau Collell C, Park S, Supek F, Muñoz J, et al. Integrated Analysis of Germline and Tumor DNA Identifies New Candidate Genes Involved in Familial Colorectal Cancer. Cancers (Basel). 2019;11: pubmed publisher
    ..i>BRCA2, BLM, ERCC2, RECQL, REV3L and RIF1 were among the most promising candidate genes for germline CRC predisposition...
  6. Bhowmick R, Thakur R, Venegas A, Liu Y, Nilsson J, Barisic M, et al. The RIF1-PP1 Axis Controls Abscission Timing in Human Cells. Curr Biol. 2019;29:1232-1242.e5 pubmed publisher
    ..Here, we identify RIF1 and its binding partner, protein phosphatase 1 (PP1), as being critical for regulation of abscission timing in ..
  7. Karachaliou N, Bracht J, Fernandez Bruno M, Drozdowskyj A, Gimenez Capitan A, Moran T, et al. Association of PALB2 Messenger RNA Expression with Platinum-Docetaxel Efficacy in Advanced Non-Small Cell Lung Cancer. J Thorac Oncol. 2019;14:304-310 pubmed publisher
    ..localizer of BRCA2 gene (PALB2), ring finger protein 8 gene (RNF8), replication timing regulatory factor 1 gene (RIF1), ATM serine/threonine kinase gene (ATM), and tumor protein p53 binding protein 1 gene (53BP1) as predictive ..
  8. Breton T, Kenter L, Greenlaw K, Montgomery J, Goetz G, Berlinsky D, et al. Initiation of sex change and gonadal gene expression in black sea bass (Centropristis striata) exposed to exemestane, an aromatase inhibitor. Comp Biochem Physiol A Mol Integr Physiol. 2019;228:51-61 pubmed publisher
    ..cyp19a1a, cyp11b), estrogen signaling (esr1, esr2b), and apoptosis or atresia (aen, casp9, fabp11, parg, pdcd4, rif1)...
  9. Hafner L, Shore D, Mattarocci S. ChECing out Rif1 action in freely cycling cells. Curr Genet. 2019;65:429-434 pubmed publisher
    ..Although many factors are involved in replication initiation, a new player, Rif1, has recently entered the scene, with a spate of papers revealing a global role for the protein in the control of ..

More Information

Publications106 found, 100 shown here

  1. Kobayashi S, Fukatsu R, Kanoh Y, Kakusho N, Matsumoto S, Chaen S, et al. Both a Unique Motif at the C Terminus and an N-Terminal HEAT Repeat Contribute to G-Quadruplex Binding and Origin Regulation by the Rif1 Protein. Mol Cell Biol. 2019;39: pubmed publisher
    b>Rif1 is a key factor for spatiotemporal regulation of DNA replication. Rif1 suppresses origin firing in the mid-late replication domains by generating replication-suppressive chromatin architecture and by recruiting a protein phosphatase...
  2. Masai H, Kakusho N, Fukatsu R, Ma Y, Iida K, Kanoh Y, et al. Molecular architecture of G-quadruplex structures generated on duplex Rif1-binding sequences. J Biol Chem. 2018;293:17033-17049 pubmed publisher
    ..A conserved protein, Rap1-interacting factor 1 (Rif1) from fission yeast (Schizosaccharomyces pombe), binds to Rif1-binding sequence (Rif1BS) and regulates DNA ..
  3. Feeser E, Wolberger C. Structural and functional studies of the Rap1 C-terminus reveal novel separation-of-function mutants. J Mol Biol. 2008;380:520-31 pubmed publisher
    ..and Sir4 proteins to chromatin via a Rap1 C-terminal domain, which also recruits the telomere length regulators, Rif1 and Rif2. We report the 1...
  4. Mei Y, Liu Y, Cao S, Tian Z, Zhou H. RIF1 promotes tumor growth and cancer stem cell-like traits in NSCLC by protein phosphatase 1-mediated activation of Wnt/?-catenin signaling. Cell Death Dis. 2018;9:942 pubmed publisher
    ..b>RIF1 has been shown upregulated in breast and cervical cancer, this study intends to find out the potential effects of ..
  5. Gao S, Feng S, Ning S, Liu J, Zhao H, Xu Y, et al. An OB-fold complex controls the repair pathways for DNA double-strand breaks. Nat Commun. 2018;9:3925 pubmed publisher
    53BP1 with its downstream proteins, RIF1, PTIP and REV7, antagonizes BRCA1-dependent homologous recombination (HR) and promotes non-homologous end joining (NHEJ) in an unclear manner...
  6. Hu Y, Wang C, Huang K, Xia F, Parvin J, Mondal N. Regulation of 53BP1 protein stability by RNF8 and RNF168 is important for efficient DNA double-strand break repair. PLoS ONE. 2014;9:e110522 pubmed publisher
    ..DNA damage sites but bulk, unbound 53BP1 remained in the nucleoplasm, and localization of its downstream effector RIF1 at DSBs was abolished...
  7. Dev H, Chiang T, Lescale C, de Krijger I, Martin A, Pilger D, et al. Shieldin complex promotes DNA end-joining and counters homologous recombination in BRCA1-null cells. Nat Cell Biol. 2018;20:954-965 pubmed publisher
    ..We establish that Shieldin acts as the downstream effector of 53BP1/RIF1/MAD2L2 to promote DNA double-strand break (DSB) end-joining by restricting DSB resection and to counteract ..
  8. Nasa I, Rusin S, Kettenbach A, Moorhead G. Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins. Sci Signal. 2018;11: pubmed publisher
    ..form of the RV[S/T]F motif (RVp[S/T]F) and used it to identify known PP1 regulatory proteins (KNL1, CDCA2, and RIF1) and multiple proteins that could potentially act as PP1 binding partners (UBR5, ASPM, SEH1, and ELYS) governed by ..
  9. Li P, Ma X, Adams I, Yuan P. A tight control of Rif1 by Oct4 and Smad3 is critical for mouse embryonic stem cell stability. Cell Death Dis. 2015;6:e1588 pubmed publisher
    ..We found that Rif1 (RAP1-associated protein 1), a factor important for genomic stability, is significantly upregulated in Smad3-/- ..
  10. Hsieh M, Tsai C, Lin C, Li T, Hung T, Chang L, et al. Topoisomerase II inhibition suppresses the proliferation of telomerase-negative cancers. Cell Mol Life Sci. 2015;72:1825-37 pubmed publisher
    ..Disturbance of telomeric structure by RIF1 or RIF2 deletion alleviates the requirement for TOP2 in telomere-telomere recombination...
  11. Lu J, Vallabhaneni H, Yin J, Liu Y. Deletion of the major peroxiredoxin Tsa1 alters telomere length homeostasis. Aging Cell. 2013;12:635-44 pubmed publisher
    ..telomerase-mediated telomere repeat addition is negatively modulated by the levels of telomere-bound Rap1-Rif1-Rif2 protein complex...
  12. Foti R, Gnan S, Cornacchia D, Dileep V, Bulut Karslioglu A, Diehl S, et al. Nuclear Architecture Organized by Rif1 Underpins the Replication-Timing Program. Mol Cell. 2016;61:260-73 pubmed publisher
    ..b>Rif1 is required for normal genome-wide regulation of replication timing, but its molecular function is poorly ..
  13. Findlay S, Heath J, Luo V, Malina A, Morin T, Coulombe Y, et al. SHLD2/FAM35A co-operates with REV7 to coordinate DNA double-strand break repair pathway choice. EMBO J. 2018;37: pubmed publisher
    ..DNA repair pathway choice is governed by the opposing activities of 53BP1, in complex with its effectors RIF1 and REV7, and BRCA1...
  14. Batenburg N, Thompson E, Hendrickson E, Zhu X. Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation. EMBO J. 2015;34:1399-416 pubmed publisher
    ..impairs the recruitment of BRCA1, RPA and Rad51 proteins to damaged chromatin but promotes the formation of 53BP1-Rif1 damage foci in S and G2 cells. Depletion of 53BP1 rescues the formation of BRCA1 damage foci in CSB-knockout cells...
  15. Noordermeer S, Adam S, Setiaputra D, Barazas M, Pettitt S, Ling A, et al. The shieldin complex mediates 53BP1-dependent DNA repair. Nature. 2018;560:117-121 pubmed publisher
    ..This function of 53BP1 requires interactions with PTIP3 and RIF14-9, the latter of which recruits REV7 (also known as MAD2L2) to break sites10,11...
  16. Dewar J, Lydall D. Similarities and differences between "uncapped" telomeres and DNA double-strand breaks. Chromosoma. 2012;121:117-30 pubmed publisher
    ..is bound by telomere "capping" proteins, which are the (Cdc13-Stn1-Ten1) CST complex, Ku (Yku70-Yku80), and Rap1-Rif1-Rif2 in budding yeast...
  17. Cooley C, Davé A, Garg M, Bianchi A. Tel1ATM dictates the replication timing of short yeast telomeres. EMBO Rep. 2014;15:1093-101 pubmed publisher
    ..of Tel1(ATM) on telomere replication timing extends to endogenous telomeres and is stronger than that elicited by Rif1 loss...
  18. Barazas M, Annunziato S, Pettitt S, de Krijger I, Ghezraoui H, Roobol S, et al. The CST Complex Mediates End Protection at Double-Strand Breaks and Promotes PARP Inhibitor Sensitivity in BRCA1-Deficient Cells. Cell Rep. 2018;23:2107-2118 pubmed publisher
    ..by the restoration of BRCA1-independent homologous recombination through loss of factors such as 53BP1, RIF1, and REV7/MAD2L2, which inhibit end resection of DNA double-strand breaks (DSBs)...
  19. Mirman Z, Lottersberger F, Takai H, Kibe T, Gong Y, Takai K, et al. 53BP1-RIF1-shieldin counteracts DSB resection through CST- and Polα-dependent fill-in. Nature. 2018;560:112-116 pubmed publisher
    ..When BRCA1 is absent, the resection of double-strand breaks is thought to be prevented by 53BP1, RIF1 and the REV7-SHLD1-SHLD2-SHLD3 (shieldin) complex, and loss of these factors diminishes sensitivity to PARP1 ..
  20. Moriyama K, Yoshizawa Sugata N, Masai H. Oligomer formation and G-quadruplex binding by purified murine Rif1 protein, a key organizer of higher-order chromatin architecture. J Biol Chem. 2018;293:3607-3624 pubmed publisher
    Rap1-interacting protein 1 (Rif1) regulates telomere length in budding yeast. We previously reported that, in metazoans and fission yeast, Rif1 also plays pivotal roles in controlling genome-wide DNA replication timing...
  21. Zhou Z, Wang L, Ge F, Gong P, Wang H, Wang F, et al. Pold3 is required for genomic stability and telomere integrity in embryonic stem cells and meiosis. Nucleic Acids Res. 2018;46:3468-3486 pubmed publisher
    ..Moreover, Pold3 mediates DNA replication and repair by regulating 53BP1, RIF1, ATR and ATM pathways...
  22. Tomida J, Takata K, Bhetawal S, Person M, Chao H, Tang D, et al. FAM35A associates with REV7 and modulates DNA damage responses of normal and BRCA1-defective cells. EMBO J. 2018;37: pubmed publisher
    ..unsolved problem is the mechanism of regulation of DNA double-strand break repair by REV7 in complex with 53BP1 and RIF1, and its influence on repair pathway choice between homologous recombination and non-homologous end-joining...
  23. Liu Y, Mei Y, Long J, Zhang Y, Hu D, Zhou H. RIF1 promotes human epithelial ovarian cancer growth and progression via activating human telomerase reverse transcriptase expression. J Exp Clin Cancer Res. 2018;37:182 pubmed publisher
    ..The aim of this study is to identify RIF1 as a novel molecular target that modulate hTERT signaling and EOC growth...
  24. Wu W, Nishikawa H, Fukuda T, Vittal V, Asano M, Miyoshi Y, et al. Interaction of BARD1 and HP1 Is Required for BRCA1 Retention at Sites of DNA Damage. Cancer Res. 2015;75:1311-21 pubmed publisher
    ..three HP1 isoforms) disrupted retention of BARD1, BRCA1, and CtIP at DSB sites and allowed ectopic accumulation of RIF1, an effector of nonhomologous end-joining, at damaged loci in S-phase...
  25. Hafner L, Lezaja A, Zhang X, Lemmens L, Shyian M, Albert B, et al. Rif1 Binding and Control of Chromosome-Internal DNA Replication Origins Is Limited by Telomere Sequestration. Cell Rep. 2018;23:983-992 pubmed publisher
    The Saccharomyces cerevisiae telomere-binding protein Rif1 plays an evolutionarily conserved role in control of DNA replication timing by promoting PP1-dependent dephosphorylation of replication initiation factors...
  26. Hiraga S, Monerawela C, Katou Y, Shaw S, Clark K, Shirahige K, et al. Budding yeast Rif1 binds to replication origins and protects DNA at blocked replication forks. EMBO Rep. 2018;19: pubmed publisher
    ..conserved function in controlling DNA replication, the chromosomal binding sites of the budding yeast Rif1 protein are not well understood...
  27. Sridhar A, Kedziora S, Donaldson A. At short telomeres Tel1 directs early replication and phosphorylates Rif1. PLoS Genet. 2014;10:e1004691 pubmed publisher
    ..The telomeric chromatin component Rif1 mediates late replication of normal telomeres and is a potential substrate of Tel1 phosphorylation, so we tested ..
  28. Piazzi M, Williamson A, Lee C, Pearson S, Lacaud G, Kouskoff V, et al. Quantitative phosphoproteome analysis of embryonic stem cell differentiation toward blood. Oncotarget. 2015;6:10924-39 pubmed
    ..factor Utf1; as well as protein specifically related to stem cell differentiation, as Eomes, Hmga2, Ints1 and Rif1. As most key factors regulating early hematopoietic development have also been implicated in various types of ..
  29. Qi Y, Zhao J, An R, Zhang J, Liang S, Shao J, et al. Mutations in circularly permuted GTPase family genes AtNOA1/RIF1/SVR10 and BPG2 suppress var2-mediated leaf variegation in Arabidopsis thaliana. Photosynth Res. 2016;127:355-67 pubmed publisher
    ..reported as Arabidopsis thaliana NITRIC OXIDE ASSOCIATED 1 (AtNOA1) and RESISTANT TO INHIBITION BY FOSMIDOMYCIN 1 (RIF1). Biochemical evidence showed that SVR10/AtNOA1/RIF1 likely localizes to the chloroplast stroma...
  30. Li B, de Lange T. Rap1 affects the length and heterogeneity of human telomeres. Mol Biol Cell. 2003;14:5060-8 pubmed
    ..scRap1 binds to telomeres with its Myb domains and uses its C terminus to recruit the telomere length regulators Rif1 and Rif2...
  31. Wang J, Aroumougame A, Lobrich M, Li Y, Chen D, Chen J, et al. PTIP associates with Artemis to dictate DNA repair pathway choice. Genes Dev. 2014;28:2693-8 pubmed publisher
    ..Since 53BP1 or its known downstream effectors, PTIP and RIF1 (RAP1-interacting factor 1 homolog), lack enzymatic activities directly implicated in DNA repair, we decided to ..
  32. Boersma V, Moatti N, Segura Bayona S, Peuscher M, van der Torre J, Wevers B, et al. MAD2L2 controls DNA repair at telomeres and DNA breaks by inhibiting 5' end resection. Nature. 2015;521:537-540 pubmed publisher
    ..End resection blocks NHEJ while committing to homology-directed repair, and is under the control of 53BP1, RIF1 and PTIP...
  33. Zhang Y, Huang H, Xu S, Wang B, Ju J, Tan H, et al. Activation and enhancement of Fredericamycin A production in deepsea-derived Streptomyces somaliensis SCSIO ZH66 by using ribosome engineering and response surface methodology. Microb Cell Fact. 2015;14:64 pubmed publisher
    ..A resistant strain ZH66-RIF1 was thereby obtained with 300 μg/mL rifampicin, which accumulated a brown pigment with cytotoxicity on MS plate ..
  34. Fukuda T, Wu W, Okada M, Maeda I, Kojima Y, Hayami R, et al. Class I histone deacetylase inhibitors inhibit the retention of BRCA1 and 53BP1 at the site of DNA damage. Cancer Sci. 2015;106:1050-6 pubmed publisher
    ..HDAC inhibitors inhibit ionizing radiation-induced foci (IRIF) formation of BRCA1 and BARD1 as well as 53BP1 and RIF1, whereas UNC0638 suppresses IRIF formation of BRCA1 and BARD1 but not 53BP1 and RIF1...
  35. Kibe T, Zimmermann M, de Lange T. TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres. Mol Cell. 2016;61:236-46 pubmed publisher
    ..DSB resection is positively and negatively regulated by ATM signaling through CtIP/MRN and 53BP1-bound Rif1, respectively...
  36. Gupta R, Somyajit K, Narita T, Maskey E, Stanlie A, Kremer M, et al. DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity. Cell. 2018;173:972-988.e23 pubmed publisher
    ..2), RINN2 (FAM35A), and RINN3 (C20ORF196). Recruitment of shieldin to DSBs, via the ATM-RNF8-RNF168-53BP1-RIF1 axis, promotes NHEJ-dependent repair of intrachromosomal breaks, immunoglobulin class-switch recombination (CSR), ..
  37. Tanaka H, Muto A, Shima H, Katoh Y, Sax N, Tajima S, et al. Epigenetic Regulation of the Blimp-1 Gene (Prdm1) in B Cells Involves Bach2 and Histone Deacetylase 3. J Biol Chem. 2016;291:6316-30 pubmed publisher
    ..nuclear co-repressors NCoR1 and NCoR2, transducin β-like 1X-linked (Tbl1x), and RAP1-interacting factor homolog (Rif1). Chromatin immunoprecipitation confirmed the binding of HDAC3 and Rif1 to the Prdm1 locus...
  38. Takikawa M, Tarumoto Y, Ishikawa F. Fission yeast Stn1 is crucial for semi-conservative replication at telomeres and subtelomeres. Nucleic Acids Res. 2017;45:1255-1269 pubmed publisher
    ..suppressor mutants of the high-temperature sensitivity of stn1-1: a multi-copy form of pmt3 and a deletion of rif1. Collectively, we propose that fission yeast Stn1 primarily safeguards the semi-conservative DNA replication at ..
  39. Cesena D, Cassani C, Rizzo E, Lisby M, Bonetti D, Longhese M. Regulation of telomere metabolism by the RNA processing protein Xrn1. Nucleic Acids Res. 2017;45:3860-3874 pubmed publisher
    ..of ssDNA generation and exerts this function by downregulating the transcript encoding the telomerase inhibitor Rif1. These findings reveal novel roles for RNA processing proteins in the regulation of telomere metabolism with ..
  40. Sreesankar E, Bharathi V, Mishra R, Mishra K. Drosophila Rif1 is an essential gene and controls late developmental events by direct interaction with PP1-87B. Sci Rep. 2015;5:10679 pubmed publisher
    b>Rif1, identified as a regulator of telomerase in yeast, is an evolutionarily conserved protein and functions in diverse processes including telomere length regulation, epigenetic gene regulation, anti-checkpoint activity, DNA repair and ..
  41. Chang M, Lin H, Luo M, Wang J, Han G. Integrated miRNA and mRNA expression profiling of tension force-induced bone formation in periodontal ligament cells. In Vitro Cell Dev Biol Anim. 2015;51:797-807 pubmed publisher
    ..WDR33, HSPH1, ERBB3, RIF1, IKBKB, CREB1, FGF2, and PAG1 were identified as hubs of the PPI network, suggesting the biological significance in ..
  42. Goto G, Zencir S, Hirano Y, Ogi H, Ivessa A, Sugimoto K. Binding of Multiple Rap1 Proteins Stimulates Chromosome Breakage Induction during DNA Replication. PLoS Genet. 2015;11:e1005283 pubmed publisher
    ..Rap1 recruits Rif1 and Rif2 to telomeres, but neither Rif1 nor Rif2 is required for DSB induction...
  43. Xue Y, Marvin M, Ivanova I, Lydall D, Louis E, Maringele L. Rif1 and Exo1 regulate the genomic instability following telomere losses. Aging Cell. 2016;15:553-62 pubmed publisher
    ..We show that Rif1 is responsible for the checkpoint tolerance and proliferation of these survivors, and that is also important for ..
  44. Buonomo S. Rif1-Dependent Regulation of Genome Replication in Mammals. Adv Exp Med Biol. 2017;1042:259-272 pubmed publisher
  45. Luijsterburg M, Typas D, Caron M, Wiegant W, van den Heuvel D, Boonen R, et al. A PALB2-interacting domain in RNF168 couples homologous recombination to DNA break-induced chromatin ubiquitylation. elife. 2017;6: pubmed publisher
    ..This cascade involves the ubiquitylation of histone H2A by the RNF168 ligase and the subsequent recruitment of RIF1, which suppresses homologous recombination (HR) in G1 cells...
  46. Wang J, Zhang H, Al Shibar M, Willard B, Ray A, Runge K. Rif1 phosphorylation site analysis in telomere length regulation and the response to damaged telomeres. DNA Repair (Amst). 2018;65:26-33 pubmed publisher
    ..b>Rif1, a telomere-binding protein, negatively regulates telomere length by forming a complex with two other telomere ..
  47. Kumar R, Cheok C. Author Correction: Dynamics of RIF1 SUMOylation is regulated by PIAS4 in the maintenance of Genomic Stability. Sci Rep. 2018;8:5236 pubmed publisher
    ..A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ..
  48. Zofall M, Smith D, Mizuguchi T, Dhakshnamoorthy J, Grewal S. Taz1-Shelterin Promotes Facultative Heterochromatin Assembly at Chromosome-Internal Sites Containing Late Replication Origins. Mol Cell. 2016;62:862-874 pubmed publisher
    ..Moreover, the late-origin regulator Rif1 affects heterochromatin at Taz1-dependent islands and subtelomeric regions...
  49. Duan Y, Zhou B, Peng J, Tong X, Zhang Q, Zhou J. Molecular dynamics of de novo telomere heterochromatin formation in budding yeast. J Genet Genomics. 2016;43:451-65 pubmed publisher
    ..Interestingly, Rif1 but not Rif2 or yKu is indispensable for the formation of intra-chromosomal silent chromatin initiated by telomeric ..
  50. Chromá K, Mistrik M, Moudry P, Gursky J, Liptay M, Strauss R, et al. Tumors overexpressing RNF168 show altered DNA repair and responses to genotoxic treatments, genomic instability and resistance to proteotoxic stress. Oncogene. 2017;36:2405-2422 pubmed publisher
    ..Moreover, the overabundant RNF168 and the ensuing unorthodox recruitment patterns of 53BP1, RIF1 and REV7 (monitored on laser micro-irradiation-induced DNA damage) shift the DSB repair balance from HR toward NHEJ,..
  51. Sukackaite R, Cornacchia D, Jensen M, Mas P, Blackledge M, Enervald E, et al. Mouse Rif1 is a regulatory subunit of protein phosphatase 1 (PP1). Sci Rep. 2017;7:2119 pubmed publisher
    b>Rif1 is a conserved protein that plays essential roles in orchestrating DNA replication timing, controlling nuclear architecture, telomere length and DNA repair...
  52. Larin M, Harding K, Williams E, Lianga N, Dore C, Pilon S, et al. Competition between Heterochromatic Loci Allows the Abundance of the Silencing Protein, Sir4, to Regulate de novo Assembly of Heterochromatin. PLoS Genet. 2015;11:e1005425 pubmed publisher
    ..Deleting RIF1 and RIF2, which suppresses the subtelomeric silencing defects in these mutants, rescues the advanced de novo ..
  53. Hiraga S, Ly T, Garzón J, Horejsi Z, Ohkubo Y, Endo A, et al. Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Rep. 2017;18:403-419 pubmed publisher
    The human RIF1 protein controls DNA replication, but the molecular mechanism is largely unknown...
  54. Yang C, Tseng S, Yu C, Chung C, Chang C, Pobiega S, et al. Telomere shortening triggers a feedback loop to enhance end protection. Nucleic Acids Res. 2017;45:8314-8328 pubmed publisher
    ..Compromised Rap1 phosphorylation hampers the interaction between Rap1 and its interacting partner Rif1, which thereby disturbs the end protection...
  55. Mattoo A, Pandita R, Chakraborty S, Charaka V, Mujoo K, Hunt C, et al. MCL-1 Depletion Impairs DNA Double-Strand Break Repair and Reinitiation of Stalled DNA Replication Forks. Mol Cell Biol. 2017;37: pubmed publisher
    ..cells exhibited decreased γ-H2AX foci, decreased phosphorylation of ATR, and higher levels of residual 53BP1 and RIF1 foci, suggesting that DNA double-strand break (DSB) repair by homologous recombination (HR) was compromised...
  56. Hass E, Zappulla D. The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae. elife. 2015;4: pubmed publisher
    ..These findings suggest that Sir4 is the telomere-bound target of Ku-mediated telomerase recruitment and provide one mechanism for how the Sir4-competing Rif1 and Rif2 proteins negatively regulate telomere length in yeast.
  57. Zhang H, Liu H, Chen Y, Yang X, Wang P, Liu T, et al. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice. Nat Commun. 2016;7:10201 pubmed publisher
    ..Subsequently, UHRF1 mediates K63-linked polyubiquitination of RIF1, and results in its dissociation from 53BP1 and DSBs thereby facilitating HR initiation...
  58. Larasati -, Duncker B. Mechanisms Governing DDK Regulation of the Initiation of DNA Replication. Genes (Basel). 2016;8: pubmed publisher
    ..Details of a mutually antagonistic relationship between DDK and Rap1-interacting factor 1 (Rif1) have also recently come to light...
  59. Moriyama K, Lai M, Masai H. Interaction of Rif1 Protein with G-Quadruplex in Control of Chromosome Transactions. Adv Exp Med Biol. 2017;1042:287-310 pubmed publisher
    ..We recently showed that Rif1, a conserved nuclear factor, binds to G4 present in the intergenic regions and plays a major role in spatiotemporal ..
  60. Fujii S, Kazama T, Ito Y, Kojima S, Toriyama K. A candidate factor that interacts with RF2, a restorer of fertility of Lead rice-type cytoplasmic male sterility in rice. Rice (N Y). 2014;7:21 pubmed publisher
    ..We identified four genes and named RF2-interacting candidate factors (RIF1 to RIF4). A study of subcellular localization demonstrated that only RIF2 was targeted to mitochondria...
  61. Chiang T, le Sage C, Larrieu D, Demir M, Jackson S. CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing. Sci Rep. 2016;6:24356 pubmed publisher
    ..We validated this approach by targeting genes for the DNA-damage response (DDR) proteins MDC1, 53BP1, RIF1 and P53, plus the nuclear architecture proteins Lamin A/C, in three different human cell lines...
  62. Zhang A, Peng B, Huang P, Chen J, Gong Z. The p53-binding protein 1-Tudor-interacting repair regulator complex participates in the DNA damage response. J Biol Chem. 2017;292:6461-6467 pubmed publisher
    ..and others have investigated the downstream effectors of 53BP1, including replication timing regulatory factor 1 (RIF1) and Pax transactivation domain-interacting protein (PTIP), in the past few years to elucidate how loss of the ..
  63. Yu T, Wang C, Lin J. Depleting components of the THO complex causes increased telomere length by reducing the expression of the telomere-associated protein Rif1p. PLoS ONE. 2012;7:e33498 pubmed publisher
    ..We found that both the mRNA and protein levels of RIF1 were decreased in tho2 and hpr1 cells...
  64. Kanoh Y, Matsumoto S, Fukatsu R, Kakusho N, Kono N, Renard Guillet C, et al. Rif1 binds to G quadruplexes and suppresses replication over long distances. Nat Struct Mol Biol. 2015;22:889-97 pubmed publisher
    b>Rif1 regulates replication timing and repair of double-strand DNA breaks. Using a chromatin immunoprecipitation-sequencing method, we identified 35 high-affinity Rif1-binding sites in fission yeast chromosomes...
  65. Ranatunga N, Forsburg S. Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4. G3 (Bethesda). 2016;6:3049-3063 pubmed publisher
    ..Surprisingly, we found that the deletion of rif1+ suppressed the MMS-sensitive phenotype without affecting temperature sensitivity...
  66. Shyian M, Mattarocci S, Albert B, Hafner L, Lezaja A, Costanzo M, et al. Budding Yeast Rif1 Controls Genome Integrity by Inhibiting rDNA Replication. PLoS Genet. 2016;12:e1006414 pubmed publisher
    The Rif1 protein is a negative regulator of DNA replication initiation in eukaryotes. Here we show that budding yeast Rif1 inhibits DNA replication initiation at the rDNA locus...
  67. Ha K, Ma C, Lin H, Tang L, Lian Z, Zhao F, et al. The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. Nat Commun. 2017;8:15751 pubmed publisher
    ..Our results suggest that the default for all DSBs is to recruit 53BP1 and RIF1. BRCA1 is blocked from being recruited to broken ends because its recruitment signal, K63-linked poly-ubiquitin ..
  68. Renaud E, Barascu A, Rosselli F. Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells. Nucleic Acids Res. 2016;44:648-56 pubmed publisher
    ..Thus, FA pathway loss-of-function results in accumulation of 53BP1, RIF1 and RAP80 at damaged chromatin, which impair DNA resection at stalled replication fork-associated DNA breaks and ..
  69. Serrano J, da Cruz e Silva O, Rebelo S. Lamina Associated Polypeptide 1 (LAP1) Interactome and Its Functional Features. Membranes (Basel). 2016;6: pubmed publisher
    ..In total, 41 interactions were identified. Several functionally relevant proteins, such as TRF2, TERF2IP, RIF1, ATM, MAD2L1 and MAD2L1BP were identified and these support the putative functions proposed for LAP1...
  70. Toteva T, Mason B, Kanoh Y, Brøgger P, Green D, Verhein Hansen J, et al. Establishment of expression-state boundaries by Rif1 and Taz1 in fission yeast. Proc Natl Acad Sci U S A. 2017;114:1093-1098 pubmed publisher
    The Shelterin component Rif1 has emerged as a global regulator of the replication-timing program in all eukaryotes examined to date, possibly by modulating the 3D-organization of the genome...
  71. Mattarocci S, Reinert J, Bunker R, Fontana G, Shi T, Klein D, et al. Rif1 maintains telomeres and mediates DNA repair by encasing DNA ends. Nat Struct Mol Biol. 2017;24:588-595 pubmed publisher
    In yeast, Rif1 is part of the telosome, where it inhibits telomerase and checkpoint signaling at chromosome ends...
  72. Alver R, Chadha G, Gillespie P, Blow J. Reversal of DDK-Mediated MCM Phosphorylation by Rif1-PP1 Regulates Replication Initiation and Replisome Stability Independently of ATR/Chk1. Cell Rep. 2017;18:2508-2520 pubmed publisher
    ..We show that DDK-dependent MCM phosphorylation is reversed by protein phosphatase 1 (PP1) targeted to chromatin by Rif1. Loss of Rif1 increased MCM phosphorylation and the rate of replication initiation and also compromised the ability ..
  73. Li L, Poon H, Hildebrandt M, Monckton E, Germain D, Fahlman R, et al. Role for RIF1-interacting partner DDX1 in BLM recruitment to DNA double-strand breaks. DNA Repair (Amst). 2017;55:47-63 pubmed publisher
    Human Rap1-interacting factor 1 (RIF1) is an important player in the repair of DNA double strand breaks (DSBs)...
  74. Xu L, Blackburn E. Human Rif1 protein binds aberrant telomeres and aligns along anaphase midzone microtubules. J Cell Biol. 2004;167:819-30 pubmed
    We identified and characterized a human orthologue of Rif1 protein, which in budding yeast interacts in vivo with the major duplex telomeric DNA binding protein Rap1p and negatively regulates telomere length...
  75. Weidemann W, Stelzl U, Lisewski U, Bork K, Wanker E, Hinderlich S, et al. The collapsin response mediator protein 1 (CRMP-1) and the promyelocytic leukemia zinc finger protein (PLZF) bind to UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE), the key enzyme of sialic acid biosynthesis. FEBS Lett. 2006;580:6649-54 pubmed
    ..For two of them, the collapsin response mediator protein 1 and the promyelocytic leukemia zinc finger protein, we could verify protein-protein interaction with GNE. ..
  76. Feng L, Fong K, Wang J, Wang W, Chen J. RIF1 counteracts BRCA1-mediated end resection during DNA repair. J Biol Chem. 2013;288:11135-43 pubmed publisher
    ..Here we report that RIF1 protein translocates to damage sites via ATM-dependent 53BP1 phosphorylation...
  77. Zaaijer S, Shaikh N, Nageshan R, Cooper J. Rif1 Regulates the Fate of DNA Entanglements during Mitosis. Cell Rep. 2016;16:148-160 pubmed publisher
    ..Here, we investigate these entanglements and their promotion by the conserved replication/repair protein Rif1. Rif1 plays no role in taz1? fork stalling...
  78. Shibata A. Regulation of repair pathway choice at two-ended DNA double-strand breaks. Mutat Res. 2017;803-805:51-55 pubmed publisher
    ..BRCA1 functions to alleviate the 53BP1 barrier to resection by promoting 53BP1 dephosphorylation, followed by RIF1 release and 53BP1 repositioning...
  79. Guo X, Bai Y, Zhao M, Zhou M, Shen Q, Yun C, et al. Acetylation of 53BP1 dictates the DNA double strand break repair pathway. Nucleic Acids Res. 2017;: pubmed publisher
    ..between 53BP1 and nucleosomes, subsequently blocking the recruitment of 53BP1 and its downstream factors PTIP and RIF1 to DSBs...
  80. Loregian A, Bortolozzo K, Boso S, Caputo A, Palu G. Interaction of Sp1 transcription factor with HIV-1 Tat protein: looking for cellular partners. FEBS Lett. 2003;543:61-5 pubmed
  81. Li P, Wang L, Bennett B, Wang J, Li J, Qin Y, et al. Rif1 promotes a repressive chromatin state to safeguard against endogenous retrovirus activation. Nucleic Acids Res. 2017;45:12723-12738 pubmed publisher
    ..Among them, Rif1 displays the strongest effect...
  82. Simonetta M, de Krijger I, Serrat J, Moatti N, Fortunato D, Hoekman L, et al. H4K20me2 distinguishes pre-replicative from post-replicative chromatin to appropriately direct DNA repair pathway choice by 53BP1-RIF1-MAD2L2. Cell Cycle. 2017;:1-29 pubmed publisher
    ..clear that DNA repair pathway choice depends on inhibition of DNA end-resection by 53BP1 and its downstream factors RIF1 and MAD2L2...
  83. GursesCila H, Acar M, Barut F, Gunduz M, Grenman R, Gunduz E. Investigation of the expression of RIF1 gene on head and neck, pancreatic and brain cancer and cancer stem cells. Clin Invest Med. 2016;39:27500 pubmed
    Recent studies have shown that cancer stem cells are resistant to chemotherapy. The aim of this study was to compare RIF1 gene expression in head and neck, pancreatic cancer and glioma cell lines and the cancer stem cells isolated from ..
  84. Isono M, Niimi A, Oike T, Hagiwara Y, Sato H, Sekine R, et al. BRCA1 Directs the Repair Pathway to Homologous Recombination by Promoting 53BP1 Dephosphorylation. Cell Rep. 2017;18:520-532 pubmed publisher
    ..We show that 53BP1 is phosphorylated by ATM in S/G2 phase, promoting RIF1 recruitment, which inhibits resection...
  85. Jongen J, van der Waals L, Trumpi K, Laoukili J, Peters N, Schenning van Schelven S, et al. Downregulation of DNA repair proteins and increased DNA damage in hypoxic colon cancer cells is a therapeutically exploitable vulnerability. Oncotarget. 2017;8:86296-86311 pubmed publisher
    ..In human tumors, expression of the repair proteins RAD51, KU70 and RIF1 was strongly suppressed in hypoxic peri-necrotic tumor areas...
  86. Lan Q, Hsiung C, Matsuo K, Hong Y, Seow A, Wang Z, et al. Genome-wide association analysis identifies new lung cancer susceptibility loci in never-smoking women in Asia. Nat Genet. 2012;44:1330-5 pubmed publisher
    ..3. We observed no evidence of association for lung cancer at 15q25 in never-smoking women in Asia, providing strong evidence that this locus is not associated with lung cancer independent of smoking. ..
  87. Hengeveld R, de Boer H, Schoonen P, de Vries E, Lens S, van Vugt M. Rif1 Is Required for Resolution of Ultrafine DNA Bridges in Anaphase to Ensure Genomic Stability. Dev Cell. 2015;34:466-74 pubmed publisher
    ..We here identify Rif1 as a factor involved in the resolution of DNA catenanes that are visible as ultrafine DNA bridges (UFBs) in ..
  88. Bakr A, Köcher S, Volquardsen J, Petersen C, Borgmann K, Dikomey E, et al. Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1. Oncotarget. 2016;7:57679-57693 pubmed publisher
    ..Here, we investigated the role of 53BP1/RIF1 in limiting BRCA1/CtIP-mediated end resection to control DSB repair pathway choice...
  89. Drané P, Brault M, Cui G, Meghani K, Chaubey S, Detappe A, et al. TIRR regulates 53BP1 by masking its histone methyl-lysine binding function. Nature. 2017;543:211-216 pubmed publisher
    ..the protein kinase ataxia-telangiectasia mutated (ATM) phosphorylates 53BP1 and recruits RAP1-interacting factor 1 (RIF1) to dissociate the 53BP1-TIRR complex...
  90. Batenburg N, Walker J, Noordermeer S, Moatti N, Durocher D, Zhu X. ATM and CDK2 control chromatin remodeler CSB to inhibit RIF1 in DSB repair pathway choice. Nat Commun. 2017;8:1921 pubmed publisher
    ..Here we uncover that CSB interacts via its newly identified winged helix domain with RIF1, an effector of 53BP1, and that this interaction mediates CSB recruitment to DSBs in S phase...
  91. Kumar R, Cheok C. RIF1: a novel regulatory factor for DNA replication and DNA damage response signaling. DNA Repair (Amst). 2014;15:54-9 pubmed publisher
    ..b>Rif1 is conserved from yeast to humans but its function has evolved from telomere length regulation in yeast to the ..
  92. Harari Y, Kupiec M. Mec1ATR is needed for extensive telomere elongation in response to ethanol in yeast. Curr Genet. 2018;64:223-234 pubmed publisher
    ..Here we show that the reduction in Rap1 protein levels disrupts the physical interaction between Rap1 and Rif1, which in turn reduces the recruitment of these two proteins to telomeres during G2-phase...