Gene Symbol: REV1
Description: REV1, DNA directed polymerase
Alias: AIBP80, REV1L, DNA repair protein REV1, REV1 homolog, REV1, polymerase (DNA directed), REV1- like, alpha integrin-binding protein 80, rev1-like terminal deoxycytidyl transferase
Species: human
Products:     REV1

Top Publications

  1. Murakumo Y, Ogura Y, Ishii H, Numata S, Ichihara M, Croce C, et al. Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7. J Biol Chem. 2001;276:35644-51 pubmed
    ..Recently, the human REV1 (hREV1) and REV3 (hREV3) genes were identified, and their products were revealed to be involved in UV-induced ..
  2. Masuda Y, Kamiya K. Role of single-stranded DNA in targeting REV1 to primer termini. J Biol Chem. 2006;281:24314-21 pubmed
    Cellular functions of the REV1 gene have been conserved in evolution and appear important for maintaining genetic integrity through translesion DNA synthesis...
  3. Edmunds C, Simpson L, Sale J. PCNA ubiquitination and REV1 define temporally distinct mechanisms for controlling translesion synthesis in the avian cell line DT40. Mol Cell. 2008;30:519-29 pubmed publisher
    ..However, in higher organisms, fully effective TLS also requires a noncatalytic function of the Y family polymerase REV1. Using the genetically tractable chicken cell line DT40, we show that TLS at stalled replication forks requires ..
  4. Swan M, Johnson R, Prakash L, Prakash S, Aggarwal A. Structure of the human Rev1-DNA-dNTP ternary complex. J Mol Biol. 2009;390:699-709 pubmed publisher
    ..The structure of yeast Rev1 ternary complex has revealed the most radical replication strategy, where the polymerase itself dictates the ..
  5. Hicks J, Chute C, Paulsen M, Ragland R, Howlett N, Gueranger Q, et al. Differential roles for DNA polymerases eta, zeta, and REV1 in lesion bypass of intrastrand versus interstrand DNA cross-links. Mol Cell Biol. 2010;30:1217-30 pubmed publisher RAD18 is necessary for efficient bypass of cisplatin adducts by the TLS polymerases eta (Poleta), REV1, and zeta (Polzeta) based on the observations that depletion of these proteins individually leads to decreased cell ..
  6. Ohashi E, Murakumo Y, Kanjo N, Akagi J, Masutani C, Hanaoka F, et al. Interaction of hREV1 with three human Y-family DNA polymerases. Genes Cells. 2004;9:523-31 pubmed
    ..Thus, our results suggest that hREV1 plays a pivotal role in the multi-enzyme, multi-step process of translesion DNA synthesis. ..
  7. Hara K, Hashimoto H, Murakumo Y, Kobayashi S, Kogame T, Unzai S, et al. Crystal structure of human REV7 in complex with a human REV3 fragment and structural implication of the interaction between DNA polymerase zeta and REV1. J Biol Chem. 2010;285:12299-307 pubmed publisher
    ..REV7 also interacts with REV1 polymerase, which is an error-prone Y family DNA polymerase and is also involved in translesion DNA synthesis...
  8. Hashimoto K, Cho Y, Yang I, Akagi J, Ohashi E, Tateishi S, et al. The vital role of polymerase ? and REV1 in mutagenic, but not correct, DNA synthesis across benzo[a]pyrene-dG and recruitment of polymerase ? by REV1 to replication-stalled site. J Biol Chem. 2012;287:9613-22 pubmed publisher
    ..Knockout of the Rev1 gene decreased both the TLS efficiency and the miscoding frequency...
  9. Guo C, Fischhaber P, Luk Paszyc M, Masuda Y, Zhou J, Kamiya K, et al. Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis. EMBO J. 2003;22:6621-30 pubmed
    Pol kappa and Rev1 are members of the Y family of DNA polymerases involved in tolerance to DNA damage by replicative bypass [translesion DNA synthesis (TLS)]. We demonstrate that mouse Rev1 protein physically associates with Pol kappa...

More Information

Publications126 found, 100 shown here

  1. McIntyre J, Sobolewska A, Fedorowicz M, McLenigan M, Macias M, Woodgate R, et al. DNA polymerase ι is acetylated in response to SN2 alkylating agents. Sci Rep. 2019;9:4789 pubmed publisher
    ..Like all other Y-family polymerases, Polι interacts with proliferating cell nuclear antigen (PCNA), Rev1, ubiquitin and ubiquitinated-PCNA and is also ubiquitinated itself...
  2. Sakamoto A, Kaya H, Endo M. Deletion of TLS polymerases promotes homologous recombination in Arabidopsis. Plant Signal Behav. 2018;13:e1483673 pubmed publisher
    ..The HR frequencies in plants deficient in DNA polymerase ζ (Pol ζ) or Rev1 were higher than that in wildtype plants under normal conditions, and were significantly increased by ultraviolet ..
  3. Vural S, Simon R, Krushkal J. Correlation of gene expression and associated mutation profiles of APOBEC3A, APOBEC3B, REV1, UNG, and FHIT with chemosensitivity of cancer cell lines to drug treatment. Hum Genomics. 2018;12:20 pubmed publisher
    ..Some studies also suggested a possible involvement of APOBEC3A, REV1, UNG, and FHIT in molecular processes affecting APOBEC mutagenesis...
  4. Kanao R, Masuda Y, Deguchi S, Yumoto Sugimoto M, Hanaoka F, Masutani C. Relevance of simultaneous mono-ubiquitinations of multiple units of PCNA homo-trimers in DNA damage tolerance. PLoS ONE. 2015;10:e0118775 pubmed publisher
    ..Notably, ectopic expression of mutant PCNA increased the UV sensitivities of Polη-proficient, Polη-deficient, and REV1-depleted cells, suggesting the disruption of a DDT pathway distinct from the Polη- and REV1-mediated pathways...
  5. Tonzi P, Huang T. Role of Y-family translesion DNA polymerases in replication stress: Implications for new cancer therapeutic targets. DNA Repair (Amst). 2019;78:20-26 pubmed publisher
    ..class of proteins is best characterized by the Y-family, encompassing DNA polymerases (Pols) Kappa, Eta, Iota, and Rev1. While best studied for their ability to bypass physical lesions on the DNA, there is accumulating evidence for ..
  6. Wojtaszek J, Lee C, D Souza S, Minesinger B, Kim H, D Andrea A, et al. Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) ζ, and Pol κ. J Biol Chem. 2012;287:33836-46 pubmed
    ..synthesis in mammalian cells is achieved in two sequential steps, with a Y-family DNA polymerase (κ, η, ι, or Rev1) inserting a nucleotide opposite the lesion and with the heterodimeric B-family polymerase ζ, consisting of the ..
  7. Destephanis D, McLeod M, Yan S. REV1 is important for the ATR-Chk1 DNA damage response pathway in Xenopus egg extracts. Biochem Biophys Res Commun. 2015;460:609-15 pubmed publisher
    The translesion DNA synthesis (TLS) polymerase REV1 is implicated in the bypass of the irreparable DNA damage such as interstrand crosslinks (ICLs)...
  8. Jansen J, Tsaalbi Shtylik A, de Wind N. Roles of mutagenic translesion synthesis in mammalian genome stability, health and disease. DNA Repair (Amst). 2015;29:56-64 pubmed publisher
    ..and DNA damage-induced nucleotide substitutions in eukaryotes depend on translesion synthesis polymerases Rev1 and Pol ζ, the latter consisting of the catalytic subunit Rev3 and the accessory protein Rev7...
  9. Suzuki M, Kino K, Kawada T, Oyoshi T, Morikawa M, Kobayashi T, et al. Contiguous 2,2,4-triamino-5(2H)-oxazolone obstructs DNA synthesis by DNA polymerases α, β, η, ι, κ, REV1 and Klenow Fragment exo-, but not by DNA polymerase ζ. J Biochem. 2016;159:323-9 pubmed publisher
    ..OzOz) using Klenow Fragment exo(-) (KF exo(-)) and DNA polymerases (Pols) α, β, ζ, η, ι, κ and REV1. We found that KF exo(-) and Pols α, β, ι and REV1 inserted one nucleotide opposite the 3' Oz of OzOz and ..
  10. Quinet A, Lerner L, Martins D, Menck C. Filling gaps in translesion DNA synthesis in human cells. Mutat Res. 2018;836:127-142 pubmed publisher
    ..Human cells count on more than eleven TLS polymerases and this work reviews the functions of some of these enzymes: Rev1, Pol η, Pol ι, Pol κ, Pol θ and Pol ζ...
  11. Chequer F, Venâncio V, Almeida M, Aissa A, Bianchi M, Antunes L. Erythrosine B and quinoline yellow dyes regulate DNA repair gene expression in human HepG2 cells. Toxicol Ind Health. 2017;33:765-774 pubmed publisher
    ..ErB (70 mg/L) significantly decreased the expression of two genes ( FEN1 and REV1) related to DNA base repair...
  12. Schiavone D, Guilbaud G, Murat P, Papadopoulou C, Sarkies P, Prioleau M, et al. Determinants of G quadruplex-induced epigenetic instability in REV1-deficient cells. EMBO J. 2014;33:2507-20 pubmed publisher
    b>REV1-deficient chicken DT40 cells are compromised in replicating G quadruplex (G4)-forming DNA. This results in localised, stochastic loss of parental chromatin marks and changes in gene expression...
  13. White C, Pone E, Lam T, Tat C, Hayama K, Li G, et al. Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses. J Immunol. 2014;193:5933-50 pubmed publisher
    ..was emphasized by unchanged expression of HoxC4 and Irf4 (important inducers/modulators of AICDA/Aicda), Rev1 and Ung (central elements for CSR/SHM), and Bcl6, Bach2, or Pax5 (repressors of PRDM1/Prdm1 expression), as well as ..
  14. Arbel Eden A, Joseph Strauss D, Masika H, Printzental O, Rachi E, Simchen G. Trans-Lesion DNA Polymerases May Be Involved in Yeast Meiosis. G3 (Bethesda). 2013;3:633-644 pubmed publisher
    ..We find that the three TLSPs of budding yeast Saccharomyces cerevisiae Rev1, PolZeta (Rev3 and Rev7), and Rad30 are induced during meiosis at a time when DNA double-strand breaks (DSBs) are ..
  15. Boehm E, Powers K, Kondratick C, Spies M, Houtman J, Washington M. The Proliferating Cell Nuclear Antigen (PCNA)-interacting Protein (PIP) Motif of DNA Polymerase η Mediates Its Interaction with the C-terminal Domain of Rev1. J Biol Chem. 2016;291:8735-44 pubmed publisher
    ..with the essential replication accessory protein proliferating cell nuclear antigen (PCNA) and the scaffold protein Rev1. In most Y-family polymerases, these interactions are mediated by one or more conserved PCNA-interacting protein (..
  16. Sharma S, Shah N, Joiner A, Roberts K, Canman C. DNA polymerase ζ is a major determinant of resistance to platinum-based chemotherapeutic agents. Mol Pharmacol. 2012;81:778-87 pubmed publisher
    ..adducts requires the cooperative actions of at least three translesion DNA synthesis (TLS) polymerases: Polη, REV1, and Polζ...
  17. Sekimoto T, Oda T, Kurashima K, Hanaoka F, Yamashita T. Both high-fidelity replicative and low-fidelity Y-family polymerases are involved in DNA rereplication. Mol Cell Biol. 2015;35:699-715 pubmed publisher
    ..Here, we report that low-fidelity Y-family polymerases (Y-Pols), Pol η, Pol ι, Pol κ, and REV1, significantly contribute to DNA synthesis during rereplication, while the replicative polymerases, Pol δ and ..
  18. Kwon S, Jin M, Cho I, Yoon J, Choi G. Identification of rehmannia virus 1, a novel putative member of the genus Closterovirus, from Rehmannia glutinosa. Arch Virol. 2018;163:3383-3388 pubmed publisher
    ..The name rehmannia virus 1 (ReV1) is proposed for this novel virus.
  19. Curina G, Nardini R, Corneli S, D Avino N, Tentellini M, Montagnoli C, et al. Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis REV1 vaccine produced in bioreactor. Vet Immunol Immunopathol. 2018;198:44-53 pubmed publisher
    The Brucella melitensis REV1 vaccine is the most widely employed vaccine for prophylaxis against brucellosis in sheep and goats. The objective of vaccination is disease control in herds or preventing infection in farms...
  20. Makarova A, Burgers P. Eukaryotic DNA polymerase ζ. DNA Repair (Amst). 2015;29:47-55 pubmed publisher
    ..Furthermore, Pol ζ forms essential interactions with the mutasome assembly factor Rev1 and with proliferating cell nuclear antigen (PCNA)...
  21. Gallina I, Christiansen S, Pedersen R, Lisby M, Oestergaard V. TopBP1-mediated DNA processing during mitosis. Cell Cycle. 2016;15:176-83 pubmed publisher
    ..evidence that unscheduled DNA synthesis in mitosis does not require the translesion synthesis scaffold factor Rev1 or PCNA ubiquitylation at K164, which serve to recruit translesion polymerases to stalled forks...
  22. Chan K, Resnick M, Gordenin D. The choice of nucleotide inserted opposite abasic sites formed within chromosomal DNA reveals the polymerase activities participating in translesion DNA synthesis. DNA Repair (Amst). 2013;12:878-89 pubmed publisher
    ..We recapitulated previous findings on the roles of REV1 and REV3. Further, we found that sequence context can strongly influence the relative frequency of A or C insertion...
  23. Ozen Z, Dash R, McCarthy K, Chow S, Rizzo A, Korzhnev D, et al. Small molecule scaffolds that disrupt the Rev1-CT/RIR protein-protein interaction. Bioorg Med Chem. 2018;26:4301-4309 pubmed publisher a heteroprotein complex consisting of specialized DNA polymerases, including the Y-family DNA polymerase Rev1. Previously, we developed a screening assay to identify the first small molecules that disrupt the protein-protein ..
  24. Powers K, Washington M. Eukaryotic translesion synthesis: Choosing the right tool for the job. DNA Repair (Amst). 2018;71:127-134 pubmed publisher
    ..complex is structurally organized by the replication accessory factor PCNA and the non-classical polymerase Rev1. One of the non-classical polymerases within this complex then catalyzes replication through the damage...
  25. Shor E, Fox C, Broach J. The yeast environmental stress response regulates mutagenesis induced by proteotoxic stress. PLoS Genet. 2013;9:e1003680 pubmed publisher
    ..Canavanine-induced mutagenesis also involves translesion DNA polymerases Rev1 and Polζ and non-homologous end joining factor Ku...
  26. Langer S, Hopfensperger K, Iyer S, Kreider E, Learn G, Lee L, et al. A Naturally Occurring rev1-vpu Fusion Gene Does Not Confer a Fitness Advantage to HIV-1. PLoS ONE. 2015;10:e0142118 pubmed publisher
    ..However, some subtype A and C viruses exhibit an unusual gene arrangement in which the first exon of rev (rev1) and the vpu gene are placed in the same open reading frame...
  27. Xu X, Lin A, Zhou C, Blackwell S, Zhang Y, Wang Z, et al. Involvement of budding yeast Rad5 in translesion DNA synthesis through physical interaction with Rev1. Nucleic Acids Res. 2016;44:5231-45 pubmed publisher
    ..Rad5 is a multi-functional protein and may also play a role in TLS, since it interacts with the TLS polymerase Rev1. In this study we mapped the Rev1-interaction domain in Rad5 to the amino acid resolution and demonstrated that ..
  28. Hoopes J, Hughes A, Hobson L, Cortez L, Brown A, Roberts S. Avoidance of APOBEC3B-induced mutation by error-free lesion bypass. Nucleic Acids Res. 2017;45:5243-5254 pubmed publisher
    ..uncovered a previously unknown role of Ubc13 in controlling the activity of the translesion synthesis polymerase, Rev1. Our results highlight a novel mechanism for error-free bypass of deoxyuridines generated within ssDNA and suggest ..
  29. Kaniak Golik A, Skoneczna A. Mitochondria-nucleus network for genome stability. Free Radic Biol Med. 2015;82:73-104 pubmed publisher
    ..In addition to mitochondria-specific DNA polymerase γ, the polymerases α, η, ζ, and Rev1 have been found in this organelle...
  30. Wu C, Spies M. G-quadruplex recognition and remodeling by the FANCJ helicase. Nucleic Acids Res. 2016;44:8742-8753 pubmed
    ..This would preserve the substrate for the REV1 translesion DNA synthesis polymerase to incorporate cytosine across from a replication-stalling G-quadruplex...
  31. Vanarotti M, Grace C, Miller D, Actis M, Inoue A, Evison B, et al. Structures of REV1 UBM2 Domain Complex with Ubiquitin and with a Small-Molecule that Inhibits the REV1 UBM2-Ubiquitin Interaction. J Mol Biol. 2018;430:2857-2872 pubmed publisher
    b>REV1 is a DNA damage tolerance protein and encodes two ubiquitin-binding motifs (UBM1 and UBM2) that are essential for REV1 functions in cell survival under DNA-damaging stress...
  32. Plachta M, Halas A, McIntyre J, Sledziewska Gojska E. The steady-state level and stability of TLS polymerase eta are cell cycle dependent in the yeast S. cerevisiae. DNA Repair (Amst). 2015;29:147-53 pubmed publisher
    ..Our results, in concert with previous data for Rev1, indicate that cell cycle regulation is a general property of Y family TLS polymerases in S. cerevisiae.
  33. Rizzo A, Vassel F, Chatterjee N, D Souza S, Li Y, Hao B, et al. Rev7 dimerization is important for assembly and function of the Rev1/Polζ translesion synthesis complex. Proc Natl Acad Sci U S A. 2018;115:E8191-E8200 pubmed publisher
    The translesion synthesis (TLS) polymerases Polζ and Rev1 form a complex that enables replication of damaged DNA...
  34. Richter K, Götz M, Winter S, Jeske H. The contribution of translesion synthesis polymerases on geminiviral replication. Virology. 2016;488:137-48 pubmed publisher
    ..a contribution of translesion synthesis (TLS) polymerases in plants, four of them (Polη, Polζ, Polκ, Rev1) are highly and constitutively expressed in differentiated tissues like the phloem...
  35. McIntyre J, Woodgate R. Regulation of translesion DNA synthesis: Posttranslational modification of lysine residues in key proteins. DNA Repair (Amst). 2015;29:166-79 pubmed publisher
    ..summarize the known lysine modifications of several key proteins involved in TLS; PCNA and Y-family polymerases η, ι, κ and Rev1 and we will discuss the potential regulatory effects of such modification in controlling TLS in vivo.
  36. Toma A, Takahashi T, Sato Y, Yamagata A, Goto Ito S, Nakada S, et al. Structural basis for ubiquitin recognition by ubiquitin-binding zinc finger of FAAP20. PLoS ONE. 2015;10:e0120887 pubmed publisher
    ..By contrast, it is reported that the attachment of a single ubiquitin to Rev1, a translesion DNA polymerase, increases binding of Rev1 to FAAP20...
  37. Boersma V, Moatti N, Segura Bayona S, Peuscher M, van der Torre J, Wevers B, et al. MAD2L2 controls DNA repair at telomeres and DNA breaks by inhibiting 5' end resection. Nature. 2015;521:537-540 pubmed publisher
    ..These activities of MAD2L2 depend on ATM kinase activity, RNF8, RNF168, 53BP1 and RIF1, but not on PTIP, REV1 and REV3, the latter two acting with MAD2L2 in translesion synthesis...
  38. Budzowska M, Graham T, Sobeck A, Waga S, Walter J. Regulation of the Rev1-pol ζ complex during bypass of a DNA interstrand cross-link. EMBO J. 2015;34:1971-85 pubmed publisher further suggest that approach is performed by a replicative polymerase, while extension involves a complex of Rev1 and DNA polymerase ζ. Rev1-pol ζ recruitment requires the Fanconi anemia core complex but not FancI-FancD2...
  39. McIntyre J, McLenigan M, Frank E, Dai X, Yang W, Wang Y, et al. Posttranslational Regulation of Human DNA Polymerase ι. J Biol Chem. 2015;290:27332-44 pubmed publisher
    ..protein, including the catalytic polymerase domain, the proliferating cell nuclear antigen-interacting region, the Rev1-interacting region, and its ubiquitin binding motifs UBM1 and UBM2...
  40. Weerasooriya S, Jasti V, Bose A, Spratt T, Basu A. Roles of translesion synthesis DNA polymerases in the potent mutagenicity of tobacco-specific nitrosamine-derived O2-alkylthymidines in human cells. DNA Repair (Amst). 2015;35:63-70 pubmed publisher
    ..siRNA knockdown of the TLS polymerases (pols) indicated that pol η, pol ζ, and Rev1 are involved in the lesion bypass of O(2)-Me-dT and O(2)-POB-dT as the TLS efficiency decreased with knockdown of ..
  41. Boehm E, Washington M. R.I.P. to the PIP: PCNA-binding motif no longer considered specific: PIP motifs and other related sequences are not distinct entities and can bind multiple proteins involved in genome maintenance. Bioessays. 2016;38:1117-1122 pubmed publisher
    ..Others include RIR motifs, which bind the translesion synthesis protein Rev1, and MIP motifs, which bind the mismatch repair protein Mlh1...
  42. Thientosapol E, Bosnjak D, Durack T, Stevanovski I, van Geldermalsen M, Holst J, et al. SAMHD1 enhances immunoglobulin hypermutation by promoting transversion mutation. Proc Natl Acad Sci U S A. 2018;115:4921-4926 pubmed publisher
    ..of uracil base excision repair (BER) and mismatch repair (MMR) then deploy translesion polymerases including REV1 and Pol η, which exacerbates mutation...
  43. Cui G, Botuyan M, Mer G. Structural Basis for the Interaction of Mutasome Assembly Factor REV1 with Ubiquitin. J Mol Biol. 2018;430:2042-2050 pubmed publisher
    b>REV1 is an evolutionarily conserved translesion synthesis (TLS) DNA polymerase and an assembly factor key for the recruitment of other TLS polymerases to DNA damage sites...
  44. Sayin Z, Uçan U, Sakmanoglu A. Antibacterial and Antibiofilm Effects of Boron on Different Bacteria. Biol Trace Elem Res. 2016;173:241-6 pubmed publisher
    ..aureus ATCC 25923, Aeromonas hydrophila ATCC 19570, Pseudomonas aeruginosa ATCC 27853, Brucella melitensis Rev1 and field isolates of Vibrio anguillarum, Aeromonas hydrophila, Yersinia ruckeri, Pseudomonas aeruginosa, ..
  45. Dai C, Chen P, Li J, Lan T, Chen Y, Qian H, et al. Co-inhibition of pol θ and HR genes efficiently synergize with cisplatin to suppress cisplatin-resistant lung cancer cells survival. Oncotarget. 2016;7:65157-65170 pubmed publisher
    ..BRCA2 and POLQ was stronger than those by co-depleting BRCA2 and other TLS factors including POLH, REV3, or REV1. Our results indicate that there is a synthetic lethal relationship between pol θ-mediated DNA repair and HR ..
  46. Zhao W, Zhao W, Zhao J, Wang D, Li J. Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens. BMC Ophthalmol. 2015;15:76 pubmed publisher
    ..Meanwhile, nine GO terms were obtained, and DEGs such as SGK1, CRY2 and REV1 were enriched in response to DNA damage stimulus...
  47. Rusz O, Pal M, Szilágyi E, Rovo L, Varga Z, Tomisa B, et al. The Expression of Checkpoint and DNA Repair Genes in Head and Neck Cancer as Possible Predictive Factors. Pathol Oncol Res. 2017;23:253-264 pubmed publisher
    ..7 cases with down-regulated REV1 expression showed complete regression after ChRT/RT, while 1 case with REV1 overexpression was resistant to RT...
  48. Wixler V, Geerts D, Laplantine E, Westhoff D, Smyth N, Aumailley M, et al. The LIM-only protein DRAL/FHL2 binds to the cytoplasmic domain of several alpha and beta integrin chains and is recruited to adhesion complexes. J Biol Chem. 2000;275:33669-78 pubmed
    ..These results, together with the fact that full-length DRAL/FHL2 is found in cell adhesion complexes, suggest that it is an adaptor/docking protein involved in integrin signaling pathways. ..
  49. Li F, Ball L, Fan L, Hanna M, Xiao W. Sgs1 helicase is required for efficient PCNA monoubiquitination and translesion DNA synthesis in Saccharomyces cerevisiae. Curr Genet. 2018;64:459-468 pubmed publisher
    ..Surprisingly, a synthetic genetic array (SGA) screen using rev1 and rev3 as baits did not reveal an anticipated synthetic effect with sgs1, indicating a possible involvement of ..
  50. Prasad R, Poltoratsky V, Hou E, Wilson S. Rev1 is a base excision repair enzyme with 5'-deoxyribose phosphate lyase activity. Nucleic Acids Res. 2016;44:10824-10833 pubmed
    b>Rev1 is a member of the Y-family of DNA polymerases and is known for its deoxycytidyl transferase activity that incorporates dCMP into DNA and its ability to function as a scaffold factor for other Y-family polymerases in translesion ..
  51. Yoon J, Hodge R, Hackfeld L, Park J, Roy Choudhury J, Prakash S, et al. Genetic control of predominantly error-free replication through an acrolein-derived minor-groove DNA adduct. J Biol Chem. 2018;293:2949-2958 pubmed publisher
    ..ring-opened reduced form of γ-HOPdG ((r) γ-HOPdG) and show that replication through this adduct is mediated via Rev1/Polη-, Polι/Polκ-, and Polθ-dependent pathways, respectively...
  52. Chattopadhyay M, Stupina V, Gao F, Szarko C, Kuhlmann M, Yuan X, et al. Requirement for Host RNA-Silencing Components and the Virus-Silencing Suppressor when Second-Site Mutations Compensate for Structural Defects in the 3' Untranslated Region. J Virol. 2015;89:11603-18 pubmed publisher
    ..mutations improved accumulation of TCV in conjunction with a partial reversion of the primary mutation (TCV-rev1) but had neutral or a negative effect on wild-type (wt) TCV or TCV with the primary mutation...
  53. Kochenova O, Bezalel Buch R, Tran P, Makarova A, Chabes A, Burgers P, et al. Yeast DNA polymerase ? maintains consistent activity and mutagenicity across a wide range of physiological dNTP concentrations. Nucleic Acids Res. 2017;45:1200-1218 pubmed publisher
    ..dNTP pools increase for Pol? function, we studied the activity and fidelity of four-subunit Pol? (Pol?4) and Pol?4-Rev1 (Pol?5) complexes in vitro at ‘normal S-phase’ and ‘damage-response’ dNTP concentrations...
  54. Santiago M, Alejandre Durán E, Ruiz Rubio M. Alternative splicing of two translesion synthesis DNA polymerases from Arabidopsis thaliana. Plant Sci. 2009;176:591-6 pubmed publisher
    ..None of the alternative AtREV1 forms recovered the yeast wild type phenotype of Rev1 deficient yeast strains after UV light irradiation or methyl methane sulphonate exposition, suggesting that AtREV1 ..
  55. Boehm E, Spies M, Washington M. PCNA tool belts and polymerase bridges form during translesion synthesis. Nucleic Acids Res. 2016;44:8250-60 pubmed publisher
    ..the ternary complexes containing proliferating cell nuclear antigen (PCNA) and two non-classical DNA polymerases, Rev1 and DNA polymerase η, have two architectures: PCNA tool belts and Rev1 bridges...
  56. Lin X, Okuda T, Trang J, Howell S. Human REV1 modulates the cytotoxicity and mutagenicity of cisplatin in human ovarian carcinoma cells. Mol Pharmacol. 2006;69:1748-54 pubmed
    b>REV1 interacts with Y-type DNA polymerases (Pol) and Pol zeta to bypass many types of adducts that block the replicative DNA polymerases...
  57. Goričar K, Kovac V, Dolzan V. Polymorphisms in translesion polymerase genes influence treatment outcome in malignant mesothelioma. Pharmacogenomics. 2014;15:941-50 pubmed publisher
    We evaluated the influence of genetic variability in translesion polymerases REV1 and REV3L on the outcome of cisplatin treatment in malignant mesothelioma patients...
  58. Sakofsky C, Ayyar S, Deem A, Chung W, Ira G, Malkova A. Translesion Polymerases Drive Microhomology-Mediated Break-Induced Replication Leading to Complex Chromosomal Rearrangements. Mol Cell. 2015;60:860-72 pubmed publisher
    ..template switches, indicative of MMBIR, are driven by translesion synthesis (TLS) polymerases Polζ and Rev1. Thus, an interruption of BIR involving fully homologous chromosomes in yeast triggers a switch to MMBIR catalyzed ..
  59. Fakouri N, Durhuus J, Regnell C, Angleys M, Desler C, Hasan Olive M, et al. Author Correction: Rev1 contributes to proper mitochondrial function via the PARP-NAD+-SIRT1-PGC1α axis. Sci Rep. 2018;8:4245 pubmed publisher
    ..A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ..
  60. Lv L, Wang F, Ma X, Yang Y, Wang Z, Liu H, et al. Mismatch repair protein MSH2 regulates translesion DNA synthesis following exposure of cells to UV radiation. Nucleic Acids Res. 2013;41:10312-22 pubmed publisher
    ..Interestingly, expression of MSH2 in Rad18-deficient cells increased UV-induced Polκ and REV1 focus formation without detectable changes in PCNA monoubiquitination, indicating that MSH2 can regulate post-UV ..
  61. Uchiyama M, Terunuma J, Hanaoka F. The Protein Level of Rev1, a TLS Polymerase in Fission Yeast, Is Strictly Regulated during the Cell Cycle and after DNA Damage. PLoS ONE. 2015;10:e0130000 pubmed publisher
    ..In fission yeast, Eso1 (polη), Kpa1/DinB (polκ), Rev1, and Polζ (a complex of Rev3 and Rev7) have been identified as translesion synthesis polymerases...
  62. Callegari A, Kelly T. Coordination of DNA damage tolerance mechanisms with cell cycle progression in fission yeast. Cell Cycle. 2016;15:261-73 pubmed publisher
    ..from DNA damage in eukaryotes are generated during DDT when cells use the mutagenic translesion polymerases, Rev1 and Polζ, rather than mechanisms with higher fidelity...
  63. Nan W, Qin L, Wang Y, Zhang Y, Tan P, Chen Y, et al. A rapid minor groove binder PCR method for distinguishing the vaccine strain Brucella abortus 104M. BMC Vet Res. 2018;14:27 pubmed publisher
    ..abortus strains S19, A19 and RB51, and B. melitensis Rev1). The main objective of this study was to develop a PCR assay for rapid and specific detection of strain 104M...
  64. Vanarotti M, Evison B, Actis M, Inoue A, McDonald E, Shao Y, et al. Small-molecules that bind to the ubiquitin-binding motif of REV1 inhibit REV1 interaction with K164-monoubiquitinated PCNA and suppress DNA damage tolerance. Bioorg Med Chem. 2018;26:2345-2353 pubmed publisher
    b>REV1 protein is a mutagenic DNA damage tolerance (DDT) mediator and encodes two ubiquitin-binding motifs (i.e., UBM1 and UBM2) that are essential for the DDT function...
  65. Kim H, Yang K, Dejsuphong D, D Andrea A. Regulation of Rev1 by the Fanconi anemia core complex. Nat Struct Mol Biol. 2012;19:164-70 pubmed publisher
    ..Recent studies indicate that the Fanconi anemia pathway also controls Rev1-mediated translesion DNA synthesis (TLS)...
  66. Liu D, Ryu K, Ko J, Sun D, Lim K, Lee J, et al. Insights into the regulation of human Rev1 for translesion synthesis polymerases revealed by the structural studies on its polymerase-interacting domain. J Mol Cell Biol. 2013;5:204-6 pubmed publisher
  67. Pande P, Malik C, Bose A, Jasti V, Basu A. Mutational analysis of the C8-guanine adduct of the environmental carcinogen 3-nitrobenzanthrone in human cells: critical roles of DNA polymerases η and κ and Rev1 in error-prone translesion synthesis. Biochemistry. 2014;53:5323-31 pubmed publisher
    ..synthesis (TLS) DNA polymerases (pols) in HEK293T cells indicated that pol η, pol κ, pol ι, pol ζ, and Rev1 each have a role in replication across this adduct...
  68. Miropolskaya N, Petushkov I, Kulbachinskiy A, Makarova A. Identification of amino acid residues involved in the dRP-lyase activity of human Pol ι. Sci Rep. 2017;7:10194 pubmed publisher
    ..were shown to possess the dRP-lyase activity and could serve as backup polymerases in base excision repair (Pol ι, Rev1, Pol γ and Pol θ)...
  69. Pozhidaeva A, Pustovalova Y, D Souza S, Bezsonova I, Walker G, Korzhnev D. NMR structure and dynamics of the C-terminal domain from human Rev1 and its complex with Rev1 interacting region of DNA polymerase η. Biochemistry. 2012;51:5506-20 pubmed
    b>Rev1 is a translesion synthesis (TLS) DNA polymerase essential for DNA damage tolerance in eukaryotes...
  70. Pustovalova Y, Bezsonova I, Korzhnev D. The C-terminal domain of human Rev1 contains independent binding sites for DNA polymerase ? and Rev7 subunit of polymerase ?. FEBS Lett. 2012;586:3051-6 pubmed publisher
    Human Rev1 is a translesion synthesis (TLS) DNA polymerase involved in bypass replication across sites of DNA damage and postreplicational gap-filling...
  71. Qin Z, Lu M, Xu X, Hanna M, Shiomi N, Xiao W. DNA-damage tolerance mediated by PCNA*Ub fusions in human cells is dependent on Rev1 but not Polη. Nucleic Acids Res. 2013;41:7356-69 pubmed publisher
    ..In contrast, depletion of Rev1, another TLS polymerase serving as a scaffold for the assembly of the TLS complex, completely abolished PCNA•Ub-..
  72. Yockey O, Jha V, Ghodke P, Xu T, Xu W, Ling H, et al. Mechanism of Error-Free DNA Replication Past Lucidin-Derived DNA Damage by Human DNA Polymerase κ. Chem Res Toxicol. 2017;30:2023-2032 pubmed publisher
    ..We examined four human pols (pol η, pol ι, pol κ, and Rev1) in their efficiency and accuracy during DNA replication with LdG; these pols are key players in translesion DNA ..
  73. Gibbs P, Wang X, Li Z, McManus T, McGregor W, Lawrence C, et al. The function of the human homolog of Saccharomyces cerevisiae REV1 is required for mutagenesis induced by UV light. Proc Natl Acad Sci U S A. 2000;97:4186-91 pubmed
    ..cerevisiae, most mutations induced by a wide range of mutagens arise during translesion replication employing the REV1 gene product and DNA polymerase zeta...
  74. Sakiyama T, Kohno T, Mimaki S, Ohta T, Yanagitani N, Sobue T, et al. Association of amino acid substitution polymorphisms in DNA repair genes TP53, POLI, REV1 and LIG4 with lung cancer risk. Int J Cancer. 2005;114:730-7 pubmed
    ..The present results suggest that these 4 SNPs function as genetic factors underlying lung cancer susceptibility by modulating activities to maintain the genome integrity of each individual. ..
  75. Eddy S, Ketkar A, Zafar M, Maddukuri L, Choi J, Eoff R. Human Rev1 polymerase disrupts G-quadruplex DNA. Nucleic Acids Res. 2014;42:3272-85 pubmed publisher
    The Y-family DNA polymerase Rev1 is required for successful replication of G-quadruplex DNA (G4 DNA) in higher eukaryotes. Here we show that human Rev1 (hRev1) disrupts G4 DNA structures and prevents refolding in vitro...
  76. Shim H, Wei M, Brandhorst S, Longo V. Starvation promotes REV1 SUMOylation and p53-dependent sensitization of melanoma and breast cancer cells. Cancer Res. 2015;75:1056-67 pubmed publisher
    ..Here, we describe the role of REV1, a specialized DNA polymerase involved in DNA repair, as an important signaling node linking nutrient sensing and ..
  77. Hara K, Taharazako S, Ikeda M, Fujita H, Mikami Y, Kikuchi S, et al. Dynamic feature of mitotic arrest deficient 2-like protein 2 (MAD2L2) and structural basis for its interaction with chromosome alignment-maintaining phosphoprotein (CAMP). J Biol Chem. 2017;292:17658-17667 pubmed publisher
    ..Structures of MAD2L2 in complex with binding regions of the TLS proteins REV3 and REV1 have revealed that MAD2L2 has two faces for protein-protein interactions that are regulated by its C-terminal ..
  78. Lin W, Xin H, Zhang Y, Wu X, Yuan F, Wang Z. The human REV1 gene codes for a DNA template-dependent dCMP transferase. Nucleic Acids Res. 1999;27:4468-75 pubmed
    ..In the yeast Saccharomyces cerevisiae, the damage-induced mutagenesis pathway requires the Rev1 protein. We have isolated a human cDNA homologous to the yeast REV1 gene...
  79. Masuda Y, Ohmae M, Masuda K, Kamiya K. Structure and enzymatic properties of a stable complex of the human REV1 and REV7 proteins. J Biol Chem. 2003;278:12356-60 pubmed
    ..It has been suggested that human REV1 interacts with human REV7, this being specific to higher eucaryotes...
  80. Piao J, Masuda Y, Kamiya K. Specific amino acid residues are involved in substrate discrimination and template binding of human REV1 protein. Biochem Biophys Res Commun. 2010;392:140-4 pubmed publisher
    b>REV1 is a member of the Y-family DNA polymerases, but is atypical in utilizing only dCTP with a preference for guanine (G) as the template...
  81. Fakouri N, Durhuus J, Regnell C, Angleys M, Desler C, Hasan Olive M, et al. Rev1 contributes to proper mitochondrial function via the PARP-NAD+-SIRT1-PGC1α axis. Sci Rep. 2017;7:12480 pubmed publisher
    ..However, translesion synthesis (TLS) DNA polymerases, such as Rev1, have the ability to bypass some DNA lesions, which can circumvent the process leading to replication fork arrest ..
  82. Choi J, Guengerich F. Kinetic analysis of translesion synthesis opposite bulky N2- and O6-alkylguanine DNA adducts by human DNA polymerase REV1. J Biol Chem. 2008;283:23645-55 pubmed publisher
    b>REV1, a Y family DNA polymerase (pol), is involved in replicative bypass past DNA lesions, so-called translesion DNA synthesis...
  83. Young R, Hopkins R, Hay B, Epton M, Mills G, Black P, et al. Lung cancer susceptibility model based on age, family history and genetic variants. PLoS ONE. 2009;4:e5302 pubmed publisher
    ..Validation in other case-control and prospective cohorts are underway to further define the potential clinical utility of this model. ..
  84. Choi J, Lim S, Kim E, Jo A, Guengerich F. Translesion synthesis across abasic lesions by human B-family and Y-family DNA polymerases ?, ?, ?, ?, ?, and REV1. J Mol Biol. 2010;404:34-44 pubmed publisher
    ..Human Y-family translesion DNA polymerases (pols) such as pols ?, ?, ?, and REV1 have been suggested to play roles in replicative bypass across many DNA lesions where B-family replicative pols ..
  85. Gabel S, Derose E, London R. XRCC1 interaction with the REV1 C-terminal domain suggests a role in post replication repair. DNA Repair (Amst). 2013;12:1105-13 pubmed
    ..Sequence analysis of the first unstructured linker domain of XRCC1 identifies a segment consistent with a possible REV1 interacting region (X1RIR) motif...
  86. Yoon J, Park J, Conde J, Wakamiya M, Prakash L, Prakash S. Rev1 promotes replication through UV lesions in conjunction with DNA polymerases η, ι, and κ but not DNA polymerase ζ. Genes Dev. 2015;29:2588-602 pubmed publisher
    ..In yeast, Rev1 plays a noncatalytic role as an indispensable component of Polζ, and Polζ together with Rev1 mediates a ..
  87. Verdiguel Fernández L, Oropeza Navarro R, Basurto Alcántara F, Castañeda Ramírez A, Verdugo Rodríguez A. Omp31 plays an important role on outer membrane properties and intracellular survival of Brucella melitensis in murine macrophages and HeLa cells. Arch Microbiol. 2017;199:971-978 pubmed publisher
    ..melitensis Bm133 wild-type and B. melitensis Rev1 vaccine strains, in assessing its susceptibility to polymyxin B, sodium deoxycholate, and nonimmune serum...
  88. Breslin C, Mani R, Fanta M, Hoch N, Weinfeld M, Caldecott K. The Rev1 interacting region (RIR) motif in the scaffold protein XRCC1 mediates a low-affinity interaction with polynucleotide kinase/phosphatase (PNKP) during DNA single-strand break repair. J Biol Chem. 2017;292:16024-16031 pubmed publisher
    ..The low-affinity interaction site required the highly conserved Rev1-interacting region (RIR) motif in XRCC1 and included three critical and evolutionarily invariant phenylalanine ..
  89. Phillips L, Sale J. The Werner's Syndrome protein collaborates with REV1 to promote replication fork progression on damaged DNA. DNA Repair (Amst). 2010;9:1064-72 pubmed publisher
    ..for lesion bypass at and behind the replication fork in the avian cell line DT40, bypass at the fork requiring REV1 and bypass at post-replicative gaps requiring PCNA ubiquitination by RAD18...
  90. Tian F, Sharma S, Zou J, Lin S, Wang B, Rezvani K, et al. BRCA1 promotes the ubiquitination of PCNA and recruitment of translesion polymerases in response to replication blockade. Proc Natl Acad Sci U S A. 2013;110:13558-63 pubmed publisher
    ..transcription factor to chromatin but also directly recruits translesion polymerases, such as Polymerase eta and Rev1, to the lesions through protein-protein interactions...
  91. Tissier A, Kannouche P, Reck M, Lehmann A, Fuchs R, Cordonnier A. Co-localization in replication foci and interaction of human Y-family members, DNA polymerase pol eta and REVl protein. DNA Repair (Amst). 2004;3:1503-14 pubmed
    ..can carry out translesion synthesis (TLS) of damaged DNA in vitro, DNA polymerases zeta, eta, iota, and kappa, and REV1. To identify novel proteins that interact with hpol eta, we performed a yeast two-hybrid screen...
  92. Masuda Y, Kamiya K. [Induced mutagenesis and translesion DNA synthesis--structure and function of REV1]. Seikagaku. 2008;80:843-6 pubmed