Genomes and Genes
Gene Symbol: rev
Species: Human immunodeficiency virus 1
- The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTPP Askjaer
Department of Molecular and Structural Biology, University of Aarhus, C F Mollers Alle, Building 130, DK 8000 Aarhus C, Denmark
J Biol Chem 273:33414-22. 1998Nuclear export of intron-containing human immunodeficiency virus type 1 (HIV-1) RNA is mediated by the viral Rev protein that contains both an RNA binding domain specific for the viral Rev response element (RRE) and a nuclear export ..
- Phosphorylation of HIV-1 Rev protein: implication of protein kinase CK2 and pro-directed kinasesF Meggio
Dipartimento di Chimica Biologica, Universita di Padova, Italy
Biochem Biophys Res Commun 226:547-54. 1996HIV-1 Rev transactivator is readily phosphorylated at separate regions by protein kinase CK2 and MAP kinase...
- Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev functionAnna Kula
Laboratory of Molecular Virology, International Centre for Genetic Engineering and Biotechnology ICGEB, Padriciano, 99, 34012 Trieste, Italy
Retrovirology 8:60. 2011..the fully competent replication cycle of the human immunodeficiency virus type 1 (HIV-1) is the nuclear export of unspliced and partially spliced RNAs mediated by the Rev posttranscriptional activator and the Rev response element (RRE).
- Inhibition of human immunodeficiency virus Rev and human T-cell leukemia virus Rex function, but not Mason-Pfizer monkey virus constitutive transport element activity, by a mutant human nucleoporin targeted to Crm1H P Bogerd
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
J Virol 72:8627-35. 1998The hypothesis that the cellular protein Crm1 mediates human immunodeficiency virus type 1 (HIV-1) Rev-dependent nuclear export posits that Crm1 can directly interact both with the Rev nuclear export signal (NES) and with cellular ..
- Perturbation of host nuclear membrane component RanBP2 impairs the nuclear import of human immunodeficiency virus -1 preintegration complex (DNA)Ruonan Zhang
Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina, United States of America
PLoS ONE 5:e15620. 2010..pore complex and has been implicated in various nucleo-cytoplasmic transport pathways including those for HIV Rev-protein. We sought to investigate the role of RanBP2 in HIV-1 replication...
- HIV-1 Rev transactivator: a beta-subunit directed substrate and effector of protein kinase CK2F Meggio
Dipartimento di Chimica Biologica, Centro di Studio delle Biomembrane del CNR, Padova, Italy
Mol Cell Biochem 227:145-51. 2001The phosphorylation of HIV-1 Rev by protein kinase CK2 is strictly dependent on the regulatory beta subunit of the kinase and is deeply affected by conformational changes of the substrate outside the phosphorylation site...
- Matrin 3 is a co-factor for HIV-1 Rev in regulating post-transcriptional viral gene expressionVenkat S R K Yedavalli
Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, The National Institutes of Health, Bethesda, Maryland 20892 0460, USA
Retrovirology 8:61. 2011..Specific signals on the viral RNAs, such as instability sequences (INS) and Rev responsive element (RRE), are binding sites for viral and cellular factors that serve to regulate RNA-export...
- Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actinW Hofmann
Department of Cell and Developmental Biology, Biocenter of the University of Wurzburg, D 97074 Wurzburg, Germany
J Cell Biol 152:895-910. 2001..Here, we investigate human immunodeficiency virus type 1 (HIV-1) Rev-mediated nuclear export and Mason-Pfizer monkey virus (MPMV) constitutive transport element (CTE)-mediated nuclear ..
- Interaction of nucleolar protein B23 with peptides related to nuclear localization signalsA Szebeni
Department of Biochemistry, University of Mississippi Medical Center, Jackson 39216 4505, USA
Biochemistry 34:8037-42. 1995..A peptide containing the sequence required for nucleolar localization of the HIV-1 Rev protein had an affinity for B23 approximately 10-fold greater than that of the SV40 T-NLS...
- Recruitment of the Crm1 nuclear export factor is sufficient to induce cytoplasmic expression of incompletely spliced human immunodeficiency virus mRNAsRui Yi
Department of Microbiology, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
J Virol 76:2036-42. 2002..that encode the late, structural proteins of human immunodeficiency virus type 1 (HIV-1) is dependent on the viral Rev regulatory protein...
- Nucleolar protein B23 stimulates nuclear import of the HIV-1 Rev protein and NLS-conjugated albuminA Szebeni
Department of Biochemistry, University of Mississippi Medical Center, Jackson 39216, USA
Biochemistry 36:3941-9. 1997..1994) J. Biol. Chem. 269, 4910-4916] using NLS peptide-conjugated bovine serum albumin (NLS-BSA) or the HIV-1 Rev protein as substrates for import into isolated rat liver nuclei...
- Distinct domains of IkappaB-alpha inhibit human immunodeficiency virus type 1 replication through NF-kappaB and RevB Y Wu
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109 0650, USA
J Virol 71:3161-7. 1997..HIV-1) replication, and unexpectedly, IkappaB-alpha was found both to suppress HIV-1 transcription and to inhibit Rev function. The relative contributions and specificities of these mechanisms to HIV replication were unknown...
- The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear exportM Neville
Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
Curr Biol 7:767-75. 1997The human immunodeficiency virus (HIV-1) uses the viral protein Rev to regulate gene expression by promoting the export of unspliced and partially spliced viral transcripts...
- The HIV-1 Rev/RRE system is required for HIV-1 5' UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particlesAdam S Cockrell
Gene Therapy Center University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA
Retrovirology 8:51. 2011..Although conventionally associated with nuclear export of HIV-1 RNA, there is a burgeoning role for the Rev/RRE in the encapsidation process...
- Nuclear RNA export and packaging functions of HIV-1 Rev revisitedMaik Blissenbach
Department of Molecular and Medical Virology, Ruhr University Bochum, Universitaetsstr 150, D 44780 Bochum, Germany
J Virol 84:6598-604. 2010Although the viral Rev protein is necessary for HIV replication, its main function in the viral replication cycle has been controversial...
- The nucleoporin nup153 plays a critical role in multiple types of nuclear exportK S Ullman
Department of Biology, University of California at San Diego, La Jolla, California 92093 0347, USA
Mol Biol Cell 10:649-64. 1999..Nup153 antibodies also block the NES protein export pathway, specifically the export of the HIV Rev protein, as well as Rev-dependent RNA export...
- A new nucleoporin-like protein interacts with both HIV-1 Rev nuclear export signal and CRM-1G Farjot
Laboratoire de Virologie, ENS Lyon, Unité INSERM U 412, 46, Allee d Italie, 69364 Lyon Cedex 07, France
J Biol Chem 274:17309-17. 1999The HIV-1 Rev is a shuttling protein required for the nuclear export of unspliced and partially spliced viral mRNA...
- Rev-dependent association of the intron-containing HIV-1 gag mRNA with the nuclear actin bundles and the inhibition of its nucleocytoplasmic transport by latrunculin-BT Kimura
Departments of Microbiology and Physiology, Kansai Medical University, Moriguchi, Osaka 570 8506, Japan
Genes Cells 5:289-307. 2000A hallmark of HIV-1 gene expression is that unspliced genomic RNA, which also acts as mRNA for the expression of Gag/Pol, is exported to the cytoplasm. Rev directs this transport through the nuclear export signal (NES).
- Identification of a domain in human immunodeficiency virus type 1 rev that is required for functional activity and modulates association with subnuclear compartments containing splicing factor SC35D M D'Agostino
Dipartimento di Scienze Oncologiche e Chirurgiche, Sezione di Oncologia, University of Padova, Italy
J Virol 74:11899-910. 2000The activity of human immunodeficiency virus Rev as a regulator of viral mRNA expression is tightly linked to its ability to shuttle between the nucleus and cytoplasm; these properties are conferred by a leucine-rich nuclear export ..
- High frequency of defective vpu compared with tat and rev genes in brain from patients with HIV type 1-associated dementiaElaine R Thomas
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
AIDS Res Hum Retroviruses 23:575-80. 2007..Here we analyzed sequences of tat, rev, and vpu genes in 55 subgenomic clones previously shown to encode functional env genes from brain and lymphoid ..
- Modulation of HIV-1 Rev protein abundance and activity by polyubiquitination with unconventional Lys-33 branchingAnne Laure Vitte
Laboratoire de Biologie Moleculaire de la Cellule, UMR5161 CNRS ENS de Lyon, IFR 128 Biosciences Lyon Gerland, 46 allee d Italie, 69364 Lyon Cedex 07, France
FEBS Lett 580:6155-60. 2006The HIV-1 Rev protein plays a key role in virus replication by allowing export to the cytoplasm of unspliced or singly-spliced RNAs. In this report, we investigated whether Rev is modified by ubiquitination or sumoylation...
- Functional analysis of the interaction of the human immunodeficiency virus type 1 Rev nuclear export signal with its cofactorsA Kiss
Institute for Molecular Virology, Saint Louis University School of Medicine, 3681 Park Avenue, Saint Louis, MO 63108, USA
Virology 314:591-600. 2003Human immunodeficiency virus type 1 (HIV-1) Rev-mediated nuclear export of viral RNAs involves the interaction of its leucine-rich nuclear export sequence (NES) with nuclear cofactors...
- Functional domains of the HIV-1 rev gene required for trans-regulation and subcellular localizationL K Venkatesh
Institute for Molecular Virology, St Louis University School of Medicine, Missouri 63110
Virology 176:39-47. 1990The rev gene of human immunodeficiency virus type 1 (HIV-1) encodes a 116 amino acid nuclear regulatory protein (Rev) that increases the cytoplasmic expression of viral mRNAs containing the Rev response element (RRE) and coding for the ..
- Binding of human prothymosin alpha to the leucine-motif/activation domains of HTLV-I Rex and HIV-1 RevS Kubota
Laboratory of Molecular Virology and Carcinogenesis, NCI Frederick Cancer Research and Development Center, MD 21702, USA
Eur J Biochem 233:48-54. 1995Rex of human T-cell leukemia virus type I (HTLV-I) and Rev of human immunodeficiency virus 1 (HIV-1) are post-transcriptional regulators of viral gene expression...
- The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAsU Fischer
Institut fur Molekularbiologie und Tumorforschung, Marburg, Federal Republic of Germany
Cell 82:475-83. 1995HIV-1 Rev protein directs nuclear export of pre-mRNAs and mRNAs containing its binding site, the Rev response element (RRE)...
- Multiple importins function as nuclear transport receptors for the Rev protein of human immunodeficiency virus type 1Marc Arnold
Universitat Heidelberg, Abteilung Virologie, Germany
J Biol Chem 281:20883-90. 2006The Rev protein of human immunodeficiency virus type 1 is an RNA-binding protein that is required for nuclear export of unspliced and partially spliced viral mRNAs...
- Nuclear Factor 90, a cellular dsRNA binding protein inhibits the HIV Rev-export functionSilvio Urcuqui-Inchima
Grupo de Inmunovirología, Corporación Biogénesis, Universidad de Antioquia, Medellin, Colombia
Retrovirology 3:83. 2006The HIV Rev protein is known to facilitate export of incompletely spliced and unspliced viral transcripts to the cytoplasm, a necessary step in virus life cycle...
- Rev inhibition strongly affects intracellular distribution of human immunodeficiency virus type 1 RNAsDusan Cmarko
Centre of Electron Microscopy, University of Lausanne, 27 Bugnon, CH 1005 Lausanne, Switzerland
J Virol 76:10473-84. 2002..RNA maturation pathways, we analyzed the intracellular distribution of HIV-1 RNA and the viral regulatory proteins Rev and Tat in transfected COS cells and HIV-1-infected lymphoid C8166 cells by means of ultrastructural in situ ..
- A human nucleoporin-like protein that specifically interacts with HIV RevC C Fritz
Howard Hughes Medical Institute, Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester 01605, USA
Nature 376:530-3. 1995The Rev protein of human immunodeficiency virus type 1 (HIV-1) facilitates the nuclear export of unspliced and partly spliced viral RNAs...
- hnRNP A1 controls HIV-1 mRNA splicing through cooperative binding to intron and exon splicing silencers in the context of a conserved secondary structureChristian Kroun Damgaard
Department of Molecular and Structural Biology, University of Aarhus, DK 8000 Aarhus C, Denmark
RNA 8:1401-15. 2002The removal of the second intron in the HIV-1 rev/tat pre-mRNAs, which involves the joining of splice site SD4 to SA7, is inhibited by hnRNP A1 by a mechanism that requires the intronic splicing silencer (ISS) and the exon splicing ..
- hRIP, a cellular cofactor for Rev function, promotes release of HIV RNAs from the perinuclear regionNuria Sánchez-Velar
University of Massachusetts Medical School, Program in Molecular Medicine and the UMASS Center for AIDS Research CFAR, Worcester, Massachusetts 01605, USA
Genes Dev 18:23-34. 2004Human immunodeficiency virus Rev facilitates the cytoplasmic accumulation of viral RNAs that contain a Rev binding site...
- A DEAD box protein facilitates HIV-1 replication as a cellular co-factor of RevJianhua Fang
Division of Infectious Diseases and Environmental Medicine, Department of Medicine, The Dorrance H Hamilton Laboratories, Center for Human Virology and Biodefense, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA
Virology 330:471-80. 2004HIV-1 Rev escorts unspliced viral mRNAs out of the nucleus of infected cells, which allows formation of infectious HIV-1 virions...
- Within you, without you: HIV-1 Rev and RNA exportAndrew I Dayton
Center for Biologics Evaluation and Research, Food and Drug Administration, USA
Retrovirology 1:35. 2004..This suggests the ubiquity of RNA helicases in RNA export from the nucleus, and has novel mechanistic implications...
- Sam68 is absolutely required for Rev function and HIV-1 productionSuhasini Modem
Department of Immunology and Microbiology, Wayne State University School of Medicine Detroit, MI 48201, USA
Nucleic Acids Res 33:873-9. 2005Sam68 functionally complements for, as well as synergizes with, HIV-1 Rev in Rev response element (RRE)-mediated gene expression and virus production...
- Regulation of HIV-1 env mRNA translation by Rev proteinCelia Perales
Centro de Biologia Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain
Biochim Biophys Acta 1743:169-75. 2005We have examined the effect of Rev on the regulation of the expression of RRE containing mRNAs when they were synthesised in the nucleus or directly in the cytoplasm...
- Identification of a novel Rev-interacting cellular proteinSusanne Kramer-Hammerle
Institute of Molecular Virology, GSF National Research Center for Environment and Health, Ingolstadter Landstrasse 1, D 85764 Neuherberg, Germany
BMC Cell Biol 6:20. 2005..The HIV Rev protein is a post-transcriptional inducer of HIV gene expression and an important target for interaction with ..
- Functional analysis of backbone cyclic peptides bearing the arm domain of the HIV-1 Rev protein: characterization of the karyophilic properties and inhibition of Rev-induced gene expressionElana Hariton-Gazal
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9104, Israel
Biochemistry 44:11555-66. 2005..the synthesis and activity of a novel backbone cyclic (BC) peptide library based on the sequence of the HIV-1 Rev arginine-rich motif (ARM)...
- Over-expression of the HIV-1 Rev promotes death of nondividing eukaryotic cellsAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
Virus Genes 40:341-6. 2010Expression of the human immunodeficiency virus type 1 (HIV-1) Rev protein is essential for completion of the viral life cycle...
- Integration of HIV-1 DNA is regulated by interplay between viral rev and cellular LEDGF/p75 proteinsAviad Levin
Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Mol Med 16:34-44. 2010The present work describes a novel interaction between the human immunodeficiency virus type 1 (HIV-1) Rev protein and the cellular lens epithelium-derived growth factor p75 (LEDGF/p75) protein in vitro and in virus-infected cells...
- Evidence for CTL-mediated selection of Tat and Rev mutants after the onset of the asymptomatic period during HIV type 1 infectionC Guillon
CNRS, FRE2736 CNRS bioMérieux, IFR128 Biosciences Lyon Gerland, Tour CERVI, 69007 Lyon, France
AIDS Res Hum Retroviruses 22:1283-92. 2006..We investigated the contribution of both selection processes to the overall evolution of the Tat and Rev regulatory gene sequences from four individuals, ranging in time from just prior to seroconversion to stable ..
- PRMT6 diminishes HIV-1 Rev binding to and export of viral RNACédric F Invernizzi
McGill University AIDS Centre, Lady Davis Institute for Medical Research, Sir Mortimer B, Davis Jewish General Hospital, Montreal, Quebec H3T 1E2, Canada
Retrovirology 3:93. 2006The HIV-1 Rev protein mediates nuclear export of unspliced and partially spliced viral RNA through interaction with the Rev response element (RRE) by means of an arginine rich motif that is similar to the one found in Tat...
- The roles of nucleolar structure and function in the subcellular location of the HIV-1 Rev proteinM Dundr
Department of Biochemistry, University of Mississippi Medical Center, Jackson 39216, USA
J Cell Sci 108:2811-23. 1995The human immunodeficiency virus 1 (HIV-1) Rev transactivator protein plays a critical role in the regulation of expression of structural proteins by controlling the pathway of mRNA transport...
- Inhibition of multidrug-resistant HIV-1 by interference with cellular S-adenosylmethionine decarboxylase activityBirgit Schäfer
Heinrich Pette Institute for Experimental Virology and Immunology, Martinistrasse, Hamburg, Germany
J Infect Dis 194:740-50. 2006..which is a cellular cofactor of the essential human immunodeficiency virus type 1 (HIV-1) regulatory protein Rev. In the present study, we show that SAM486A efficiently suppresses HIV-1 replication, including the replication of ..
- RTE and CTE mRNA export elements synergistically increase expression of unstable, Rev-dependent HIV and SIV mRNAsSergey Smulevitch
Human Retrovirus Pathogenesis Section, National Cancer Institute Frederick, MD 21702 1201, USA
Retrovirology 3:6. 2006..RTE and CTE are potent in nucleocytoplasmic mRNA transport and expression, neither element is as powerful as the Rev-RRE posttranscriptional control...
- Requirement of an additional Sam68 domain for inhibition of human immunodeficiency virus type 1 replication by Sam68 dominant negative mutants lacking the nuclear localization signalJizhong Zhang
Department of Microbiology and Immunology, Indiana University School of Medicine, R2 302, 950 W Walnut St, Indianapolis, IN 46202, United States
Gene 363:67-76. 2005..This process is evolutionally made possible by expression of HIV-1 Rev, one of the three HIV-1 proteins encoded by completely spliced HIV-1 RNAs...
- HIV-1 rev depolymerizes microtubules to form stable bilayered ringsN R Watts
Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, MD, USA
J Cell Biol 150:349-60. 2000We describe a novel interaction between HIV-1 Rev and microtubules (MTs) that results in the formation of bilayered rings that are 44-49 nm in external diameter, 3.4-4.2 MD (megadaltons) in mass, and have 28-, 30-, or 32-fold symmetry...
- Synergistic stimulation of HIV-1 rev-dependent export of unspliced mRNA to the cytoplasm by hnRNP A1I Najera
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
J Mol Biol 285:1951-64. 1999..Efficient transport of these mRNAs from the nucleus requires the binding of the viral nuclear transport protein Rev to an RNA stem-loop structure called the RRE (Rev response element)...
- Nucleoporins nup98 and nup214 participate in nuclear export of human immunodeficiency virus type 1 RevA S Zolotukhin
Human Retrovirus Pathogenesis Group, ABL Basic Research Program, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, Maryland 21702 1201, USA
J Virol 73:120-7. 1999Human immunodeficiency virus type 1 (HIV-1) Rev contains a leucine-rich nuclear export signal that is essential for its nucleocytoplasmic export mediated by hCRM1...
- Nuclear export of late HIV-1 mRNAs occurs via a cellular protein export pathwayR A Fridell
Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
Proc Natl Acad Sci U S A 93:4421-4. 1996The Rev protein of HIV-1 is essential for the nuclear export of incompletely spliced viral mRNAs...
- In vitro interaction between human immunodeficiency virus type 1 Rev protein and splicing factor ASF/SF2-associated protein, p32T O Tange
Department of Molecular Biology, University of Aarhus, C F Møllers Alle, Building 130, DK 8000 Aarhus C, Denmark
J Biol Chem 271:10066-72. 1996Continuous replication of human immunodeficiency virus type 1 requires the expression of the regulatory protein Rev, which binds to the Rev response element (RRE) and up-regulates the cytoplasmic appearance of singly spliced and ..
- Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activationM Ruhl
Sandoz Research Institute, Vienna, Austria
J Cell Biol 123:1309-20. 1993..virus type 1 (HIV-1) structural proteins requires the presence of the viral trans-activator protein Rev. Rev is localized in the nucleus and binds specifically to the Rev response element (RRE) sequence in viral RNA...
- Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1A S Zolotukhin
Human Retrovirus Pathogenesis Group, NCI Frederick Cancer Research and Development Center, ABL Basic Research Program, Frederick, Maryland 21702 1201, USA
J Biol Chem 272:11356-60. 1997..is responsible for the cytoplasmic accumulation of RanBP1 and can functionally replace the nuclear export signal of Rev of human immunodeficiency virus type 1...
- The basic domain of Rev from human immunodeficiency virus type 1 specifically blocks the entry of U4/U6.U5 small nuclear ribonucleoprotein in spliceosome assemblyJ Kjems
Department of Molecular Biology, University of Aarhus, Denmark
J Virol 67:4769-76. 1993Human immunodeficiency virus type 1 (HIV-1) encodes a regulatory protein, Rev, which is required for cytoplasmic expression of incompletely spliced viral mRNA...
- HIV-1 Rev is capable of shuttling between the nucleus and cytoplasmN Richard
Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada
Virology 204:123-31. 1994The mechanism by which Rev facilitates the export, and consequently, the translation of the structural protein mRNAs of the human immunodeficiency virus type 1 remains undefined...
- A role for Rev in the association of HIV-1 gag mRNA with cytoskeletal beta-actin and viral protein expressionT Kimura
Department of Microbiology, Kansai Medical University, Osaka, Japan
Biochimie 78:1075-80. 1996Human immunodeficiency virus type-1 (HIV-1) Rev acts by inducing the specific nucleocytoplasmic transport of a class of incompletely spliced RNAs that encodes the viral structural proteins...
- Specific binding of polypyrimidine tract binding protein and hnRNP A1 to HIV-1 CRS elementsA C Black
Virus Genes 12:275-85. 1996The human immunodeficiency virus (HIV) Rev and human T-cell leukemia virus (HTLV) Rex proteins regulate viral RNA processing...
- Inhibition of HIV replication by dominant negative mutants of Sam68, a functional homolog of HIV-1 RevT R Reddy
Department of Medicine, University of California San Diego, La Jolla 92093, USA
Nat Med 5:635-42. 1999The HIV-1 Rev protein facilitates the nuclear export of mRNA containing the Rev response element (RRE) through binding to the export receptor CRM-1...
- Eukaryotic initiation factor 5A activity and HIV-1 Rev functionD Bevec
Novartis Research Institute, Department of Immunodermatology, Vienna, Austria
Biol Signals 6:124-33. 1997..The unexpected finding that eIF-5A is a cellular cofactor of the HIV-1 Rev trans-activator protein may, however, provide a novel opportunity to reveal precisely what function eIF-5A performs ..
- Interaction of eukaryotic initiation factor 5A with the human immunodeficiency virus type 1 Rev response element RNA and U6 snRNA requires deoxyhypusine or hypusine modificationY P Liu
Department of Chemistry, Rutgers State University of New Jersey 08855 0939, USA
Biol Signals 6:166-74. 1997..Recent studies have suggested that eIF-5A may be the cellular target of the human immunodeficiency virus type 1 Rev and human T cell leukemia virus type 1 Rex proteins...
- Nuclear pore localization and nucleocytoplasmic transport of eIF-5A: evidence for direct interaction with the export receptor CRM1O Rosorius
Institute for Clinical and Molecular Virology, University Erlangen Nurnberg, Schlossgarten 4, D 91054 Erlangen, Germany
J Cell Sci 112:2369-80. 1999..The finding that eIF-5A is an essential cofactor of the human immunodeficiency virus type 1 (HIV-1) Rev RNA transport factor suggested that eIF-5A is part of a specific nuclear export pathway...
- Signal peptide fragments of preprolactin and HIV-1 p-gp160 interact with calmodulinB Martoglio
Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, Postfach 106249, 69052 Heidelberg, Germany
EMBO J 16:6636-45. 1997..The functional consequences of such interactions remain to be established. However, our data suggest that signal sequences may be functionally more versatile than anticipated up to now...
- A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleusF Stutz
Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
Mol Cell Biol 16:7144-50. 1996The human immunodeficiency virus type 1 Rev protein contains a nuclear export signal (NES) that is required for Rev-mediated RNA export in mammals as well as in the yeast Saccharomyces cerevisiae...
- Stable complex formation between HIV Rev and the nucleosome assembly protein, NAP1, affects Rev functionAlan Cochrane
Department of Molecular Genetics, University of Toronto, Toronto, Canada
Virology 388:103-11. 2009The Rev protein of HIV-1 is essential for HIV-1 proliferation due to its role in exporting viral RNA from the nucleus...
- Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in the HIV Rev proteinKamyar Hadian
Institute of Virology, Helmholtz Zentrum Munchen, Research Center for Environmental Health, Ingolstaedter Landstrasse 1, D 85764 Neuherberg, Germany
J Biol Chem 284:33384-91. 2009The Rev protein is a key regulator of human immunodeficiency virus type 1 (HIV-1) gene expression. Rev is primarily known as an adaptor protein for nuclear export of HIV RNAs...
- HIV Rev-dependent binding of SF2/ASF to the Rev response element: possible role in Rev-mediated inhibition of HIV RNA splicingD M Powell
Gladstone Institute of Virology and Immunology, University of California, San Francisco 94141 9100, USA
Proc Natl Acad Sci U S A 94:973-8. 1997..of the structural and enzymatic proteins of type 1 human immunodeficiency virus (HIV-1) is controlled by the rev regulatory gene product...
- The human HIV-1 Rev binding-protein hRIP/Rab (HRB) maps to chromosome 2q36T Jones
Sandoz Research Institute, Vienna, Austria
Genomics 40:198-9. 1997
- Localization of human immunodeficiency virus Rev in transfected and virus-infected cellsL Luznik
Department of Medicine, UCSD School of Medicine, La Jolla, California 92093 0665, USA
AIDS Res Hum Retroviruses 11:795-804. 1995The rev gene product of human immunodeficiency virus (HIV) is obligatory for viral replication. Rev interacts specifically with a structured RNA sequence within the viral genome termed the REV response element (RRE)...
- Expression of (cac)n/(gtg)n simple repetitive sequences in mRNA of human lymphocytesC Epplen
Ruhr Universitat Bochum, Germany
Hum Genet 93:35-41. 1994..Longer stretches of perfect simple (cac)n/(gtg)n repeats can rarely be recovered, even from the hnRNA of human lymphocytes...
- Identification of sequences important in the nucleolar localization of human immunodeficiency virus Rev: relevance of nucleolar localization to functionA W Cochrane
Department of Molecular Oncology, Roche Research Center, Nutley, New Jersey 07110
J Virol 64:881-5. 1990The human immunodeficiency virus rev gene product regulates the expression of viral structural genes. It was recently shown that Rev regulates the export of viral structural mRNAs from the nucleus to the cytoplasm...
- Phenotypic analysis of human immunodeficiency virus type 1 Rev trimerization-interface mutants in human cellsRoochi Trikha
The Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, UK
J Gen Virol 86:1509-13. 2005..export of unspliced and incompletely spliced human immunodeficiency virus type 1 mRNA is mediated by the viral Rev protein...
- Rev protein suppression of complex formation between nuclear proteins and rev-responsive element-containing RNA of human immunodeficiency virus-1A Bek
, , Mainz, Germany
Int J Biochem Cell Biol 27:1317-29. 1995
- The RNA helicase DDX1 is involved in restricted HIV-1 Rev function in human astrocytesJianhua Fang
The Dorrance H Hamilton Laboratories, Center for Human Virology and Biodefense, Division of Infectious Diseases and Environmental Medicine, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA
Virology 336:299-307. 2005..Of note, a low ratio of unspliced versus spliced HIV-1-specific RNA was also investigated, as Rev appeared to act aberrantly in astrocytes, via loss of nuclear and/or nucleolar localization and diminished Rev-..
- Unique features of HIV-1 Rev protein phosphorylation by protein kinase CK2 ('casein kinase-2')O Marin
Dipartmento di Chimica Biologica and Centro del CNR per lo Studio delle Biomembrane, Università delgi Studi di Padova, Padua, Italy
FEBS Lett 481:63-7. 2000The HIV-1 Rev transactivator is phosphorylated in vitro by protein kinase CK2 at two residues, Ser-5 and Ser-8; these sites are also phosphorylated in vivo...
- Regulation of human retroviral latency by the NF-kappa B/I kappa B family: inhibition of human immunodeficiency virus replication by I kappa B through a Rev-dependent mechanismB Y Wu
Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109 0650
Proc Natl Acad Sci U S A 92:1480-4. 1995..I kappa B alpha inhibited HIV replication and gp160 expression by negatively regulating Rev function, most likely acting through a cellular factor involved in Rev transactivation...
- "Tissue" transglutaminase expression in HIV-infected cells: an enzyme with an antiviral effect?A Amendola
Laboratory of Virology, Lazzaro Spallanzani IRCCS, Rome, Italy
Ann N Y Acad Sci 946:108-20. 2001..This effect might be due to its specific interaction with eIF5A, a cellular partner of HIV Rev protein, which is essential for HIV replication in immune-competent cells...
- Nuclear export of the human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev is mediated by its activation domain and is blocked by transdominant negative mutantsA M Szilvay
National Centre for Research in Virology, Gade Institute, University of Bergen, Norway
J Virol 69:3315-23. 1995The human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev moves repeatedly between the cytoplasm, a perinuclear zone, the nucleoli, and nucleoplasmic speckles...
- Cytotoxic activity of rev protein of human immunodeficiency virus type 1 by nucleolar dysfunctionT Nosaka
Institute for Virus Research, Kyoto University, Japan
Exp Cell Res 209:89-102. 1993The rev protein (Rev) of the human immunodeficiency virus type 1 (HIV-1) is known as a post-transcriptional regulator of viral gene expression. It is located in the cell nucleolus...
- The HIV-1 Rev trans-activator shuttles between the nucleus and the cytoplasmB E Meyer
Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148
Genes Dev 8:1538-47. 1994The HIV-1 Rev protein is a nuclear trans-activator essential for the transport of unspliced viral transcripts to the cytoplasm...
- Role of protein kinase CK2 phosphorylation in the molecular chaperone activity of nucleolar protein b23Attila Szebeni
Department of Biochemistry, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA
J Biol Chem 278:9107-15. 2003..The dissociation of complexes between B23 and the human immunodeficiency virus-Rev protein required both CK2 phosphorylation and competition with a Rev nuclear localization signal peptide, ..
- Human p32 protein relieves a post-transcriptional block to HIV replication in murine cellsYong Hui Zheng
Department of Medicine, Rosalind Russell Medical Research Center, Mt Zion Research Building Room N231, 2340 Sutter Street, University of California, San Francisco, CA 94115, USA
Nat Cell Biol 5:611-8. 2003..A single substitution in hp32 of Gly 35 to Asp 35, which is found in mp32, abrogates this activity. Thus, hp32 overcomes an important post-transcriptional block to HIV replication in murine cells...
- Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteinsH P Bogerd
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
Cell 82:485-94. 1995HIV-1 Rev is the prototype of a class of retroviral regulatory proteins that induce the sequence-specific nuclear export of target RNAs...
- A bidirectional SF2/ASF- and SRp40-dependent splicing enhancer regulates human immunodeficiency virus type 1 rev, env, vpu, and nef gene expressionMassimo Caputi
Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
J Virol 78:6517-26. 2004..a novel bidirectional exonic splicing enhancer (ESE) that regulates the expression of the HIV-1 env, vpu, rev, and nef mRNAs. The ESE is localized downstream of the vpu-, env-, and nef-specific 3' splice site no. 5...
- Automatic and quantitative measurement of protein-protein colocalization in live cellsSylvain V Costes
Image Analysis Laboratory, National Cancer Institute, Frederick, Maryland, USA
Biophys J 86:3993-4003. 2004..was further illustrated by measuring, for the first time in live cells, the dissociation rate (k(d)) of the HIV-1 Rev/CRM1 export complex induced by the cytotoxin leptomycin B...
- General effect of Sam68 on Rev/Rex regulated expression of complex retrovirusesT R Reddy
Department of Medicine, University of California San Diego, La Jolla, California, CA 92093 0665, USA
Oncogene 19:4071-4. 2000..demonstrated that overexpression of Sam68 functionally substitutes for, as well as synergizes with, HIV-1 Rev in RRE-mediated gene expression and virus replication...
- Subcellular localization of human immunodeficiency virus type 1 RNAs, Rev, and the splicing factor SC-35S O Bøe
Department of Microbiology and Immunology, Gade Institute, Bergen High Technology Center, University of Bergen, Norway
Virology 244:473-82. 1998The HIV-1 protein Rev regulates the cytoplasmic levels of incompletely spliced HIV-1 mRNAs. The plasmid pSVc21, which contains a HIV-1 provirus, was introduced into COS cells by transient transfection...
- The cytotoxicity of human immunodeficiency virus type 1 Rev: implications for its interaction with the nucleolar protein B23Y Miyazaki
Institute for Virus Research, Kyoto University, Japan
Exp Cell Res 219:93-101. 1995..type 1 (HIV-1) encodes several regulatory proteins, including two essential trans-activators for viral replication, Rev and Tat. Both Rev and Tat have a nucleolar targeting signal and are actually located predominantly in the nucleoli...
- Human immunodeficiency virus type 1 (HIV-1) Vpr-regulated cell death: insights into mechanismK Muthumani
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Cell Death Differ 12:962-70. 2005..Further, we also discuss caveats and future directions for investigation of the interesting biology of this HIV accessory gene...
- Histographic recording of human immunodeficiency virus type 1 (HIV-1) regulatory protein Rev and nuclear factorsA Kaneström
Department of Microbiology and Immunology, Gade Institute, University of Bergen, High Technology Center of Bergen, Norway
Arch Virol 143:279-94. 1998HeLa cells and HeLa cells expressing the HIV-1 regulatory protein Rev were immunostained for Rev and pre-mRNA processing factors and examined histographically by confocal laser scanning microscopy...
- Sam68, RNA helicase A and Tap cooperate in the post-transcriptional regulation of human immunodeficiency virus and type D retroviral mRNAT R Reddy
Department of Medicine, University of California, San Diego, La Jolla 92093 0665, USA
Oncogene 19:3570-5. 2000..nuclear export pathways for retroviral mRNA have been described: a CRM-1 dependent pathway mediated by the HIV-1 Rev protein and the Rev Response Element (RRE), and a CRM-1 independent pathway mediated by the Constitutive Transport ..
- Site-specific phosphorylation of the human immunodeficiency virus type-1 Rev protein accelerates formation of an efficient RNA-binding conformationD E Fouts
Department of Microbiology and College of Medicine, University of Illinois at Urbana Champaign, USA
Biochemistry 36:13256-62. 1997..In this report, we demonstrate that phosphorylation of a recombinant human immunodeficiency virus type-1 Rev protein promotes rapid formation of an efficient RNA-binding state...
- The Nuclear Pore Complex: Interphase and Mitotic FunctionKATHARINE ULLMAN; Fiscal Year: 2007..The information gathered will impact practical applications, such as optimizing expression of exogenously introduced genes as well as understanding -and ultimately controlling-growth deregulation found in cancerous cells. ..
- MOLECULAR MECHANISMS OF NUCLEAR EXPORTKATHARINE ULLMAN; Fiscal Year: 2004..Through genetic and biochemical interaction analyses, Nup153 will be used to construct a bigger picture of key connections made between export machinery components. ..
- The Nuclear Pore Complex: Interphase and Mitotic FunctionKatharine S Ullman; Fiscal Year: 2010..Such defects in morphology and DNA content are hallmarks of aggressive cancer cells, and the research proposed here will contribute to a better understanding of how these alterations arise. ..