rev

Summary

Gene Symbol: rev
Description: p19
Alias: Rev
Species: Human immunodeficiency virus 1

Top Publications

  1. pmc Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev function
    Anna Kula
    Laboratory of Molecular Virology, International Centre for Genetic Engineering and Biotechnology ICGEB, Padriciano, 99, 34012 Trieste, Italy
    Retrovirology 8:60. 2011
  2. pmc Structural basis for cooperative RNA binding and export complex assembly by HIV Rev
    Matthew D Daugherty
    Chemistry and Chemical Biology Graduate Program, University of California, San Francisco, San Francisco, California, USA
    Nat Struct Mol Biol 17:1337-42. 2010
  3. pmc Trimethylguanosine capping selectively promotes expression of Rev-dependent HIV-1 RNAs
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0460, USA
    Proc Natl Acad Sci U S A 107:14787-92. 2010
  4. ncbi Cellular proteins and HIV-1 Rev function
    Modem Suhasini
    Department of Immunology and Microbiology, Wayne State University, Detroit, MI, USA
    Curr HIV Res 7:91-100. 2009
  5. pmc Matrin 3 is a co-factor for HIV-1 Rev in regulating post-transcriptional viral gene expression
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, The National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    Retrovirology 8:61. 2011
  6. pmc Nuclear RNA export and packaging functions of HIV-1 Rev revisited
    Maik Blissenbach
    Department of Molecular and Medical Virology, Ruhr University Bochum, Universitaetsstr 150, D 44780 Bochum, Germany
    J Virol 84:6598-604. 2010
  7. pmc Specific complex of human immunodeficiency virus type 1 rev and nucleolar B23 proteins: dissociation by the Rev response element
    C Fankhauser
    Department of Biochemistry, University of Geneva, Switzerland
    Mol Cell Biol 11:2567-75. 1991
  8. pmc Single-molecule studies reveal that DEAD box protein DDX1 promotes oligomerization of HIV-1 Rev on the Rev response element
    Rae M Robertson-Anderson
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Mol Biol 410:959-71. 2011
  9. pmc The HIV-1 Rev/RRE system is required for HIV-1 5' UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particles
    Adam S Cockrell
    Gene Therapy Center University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA
    Retrovirology 8:51. 2011
  10. pmc PRMT6 diminishes HIV-1 Rev binding to and export of viral RNA
    Cédric F Invernizzi
    McGill University AIDS Centre, Lady Davis Institute for Medical Research, Sir Mortimer B, Davis Jewish General Hospital, Montreal, Quebec H3T 1E2, Canada
    Retrovirology 3:93. 2006

Scientific Experts

Detail Information

Publications111 found, 100 shown here

  1. pmc Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev function
    Anna Kula
    Laboratory of Molecular Virology, International Centre for Genetic Engineering and Biotechnology ICGEB, Padriciano, 99, 34012 Trieste, Italy
    Retrovirology 8:60. 2011
    ..the fully competent replication cycle of the human immunodeficiency virus type 1 (HIV-1) is the nuclear export of unspliced and partially spliced RNAs mediated by the Rev posttranscriptional activator and the Rev response element (RRE).
  2. pmc Structural basis for cooperative RNA binding and export complex assembly by HIV Rev
    Matthew D Daugherty
    Chemistry and Chemical Biology Graduate Program, University of California, San Francisco, San Francisco, California, USA
    Nat Struct Mol Biol 17:1337-42. 2010
    ..Export is mediated by cooperative binding of the Rev protein to the Rev response element (RRE) RNA, to form a highly specific oligomeric ribonucleoprotein (RNP) that ..
  3. pmc Trimethylguanosine capping selectively promotes expression of Rev-dependent HIV-1 RNAs
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 0460, USA
    Proc Natl Acad Sci U S A 107:14787-92. 2010
    ..CRM-1 is also used to export Rev/RRE-dependent unspliced/ partially spliced HIV-1 RNAs...
  4. ncbi Cellular proteins and HIV-1 Rev function
    Modem Suhasini
    Department of Immunology and Microbiology, Wayne State University, Detroit, MI, USA
    Curr HIV Res 7:91-100. 2009
    ..This process, mediated by the Rev protein, interfaces with cellular components involved in post-transcriptional gene regulation...
  5. pmc Matrin 3 is a co-factor for HIV-1 Rev in regulating post-transcriptional viral gene expression
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, The National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    Retrovirology 8:61. 2011
    ..Specific signals on the viral RNAs, such as instability sequences (INS) and Rev responsive element (RRE), are binding sites for viral and cellular factors that serve to regulate RNA-export...
  6. pmc Nuclear RNA export and packaging functions of HIV-1 Rev revisited
    Maik Blissenbach
    Department of Molecular and Medical Virology, Ruhr University Bochum, Universitaetsstr 150, D 44780 Bochum, Germany
    J Virol 84:6598-604. 2010
    Although the viral Rev protein is necessary for HIV replication, its main function in the viral replication cycle has been controversial...
  7. pmc Specific complex of human immunodeficiency virus type 1 rev and nucleolar B23 proteins: dissociation by the Rev response element
    C Fankhauser
    Department of Biochemistry, University of Geneva, Switzerland
    Mol Cell Biol 11:2567-75. 1991
    The human immunodeficiency virus type 1 (HIV) Rev protein is thought to be involved in the export of unspliced or singly spliced viral mRNAs from the nucleus to the cytoplasm...
  8. pmc Single-molecule studies reveal that DEAD box protein DDX1 promotes oligomerization of HIV-1 Rev on the Rev response element
    Rae M Robertson-Anderson
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Mol Biol 410:959-71. 2011
    Oligomeric assembly of Rev on the Rev response element (RRE) is essential for the nuclear export of unspliced and singly spliced human immunodeficiency virus type 1 viral mRNA transcripts...
  9. pmc The HIV-1 Rev/RRE system is required for HIV-1 5' UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particles
    Adam S Cockrell
    Gene Therapy Center University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA
    Retrovirology 8:51. 2011
    ..Although conventionally associated with nuclear export of HIV-1 RNA, there is a burgeoning role for the Rev/RRE in the encapsidation process...
  10. pmc PRMT6 diminishes HIV-1 Rev binding to and export of viral RNA
    Cédric F Invernizzi
    McGill University AIDS Centre, Lady Davis Institute for Medical Research, Sir Mortimer B, Davis Jewish General Hospital, Montreal, Quebec H3T 1E2, Canada
    Retrovirology 3:93. 2006
    The HIV-1 Rev protein mediates nuclear export of unspliced and partially spliced viral RNA through interaction with the Rev response element (RRE) by means of an arginine rich motif that is similar to the one found in Tat...
  11. pmc Inhibition of human immunodeficiency virus Rev and human T-cell leukemia virus Rex function, but not Mason-Pfizer monkey virus constitutive transport element activity, by a mutant human nucleoporin targeted to Crm1
    H P Bogerd
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Virol 72:8627-35. 1998
    The hypothesis that the cellular protein Crm1 mediates human immunodeficiency virus type 1 (HIV-1) Rev-dependent nuclear export posits that Crm1 can directly interact both with the Rev nuclear export signal (NES) and with cellular ..
  12. ncbi Synergistic stimulation of HIV-1 rev-dependent export of unspliced mRNA to the cytoplasm by hnRNP A1
    I Najera
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    J Mol Biol 285:1951-64. 1999
    ..Efficient transport of these mRNAs from the nucleus requires the binding of the viral nuclear transport protein Rev to an RNA stem-loop structure called the RRE (Rev response element)...
  13. pmc The nucleoporin nup153 plays a critical role in multiple types of nuclear export
    K S Ullman
    Department of Biology, University of California at San Diego, La Jolla, California 92093 0347, USA
    Mol Biol Cell 10:649-64. 1999
    ..Nup153 antibodies also block the NES protein export pathway, specifically the export of the HIV Rev protein, as well as Rev-dependent RNA export...
  14. pmc Rev-ing up post-transcriptional HIV-1 RNA expression
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, The National Institutes of Health, Bethesda, MD, USA
    RNA Biol 8:195-9. 2011
    ..HIV-1 has evolved a protein, Rev, that participates in the export of unspliced / partially spliced viral RNAs from the nucleus...
  15. pmc Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: novel insights into the regulation of Rev nuclear import
    Lili Gu
    UCD Centre for Research in Infectious Diseases, School of Medicine and Medical Science, University College Dublin UCD, Belfield, Dublin 4, Ireland
    Retrovirology 8:17. 2011
    The HIV-1 regulatory protein Rev, which is essential for viral replication, mediates the nuclear export of unspliced viral transcripts...
  16. pmc The cellular HIV-1 Rev cofactor hRIP is required for viral replication
    Zhong Yu
    Program in Molecular Medicine and Center for AIDS Research CFAR, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 102:4027-32. 2005
    ..The HIV-1 Rev protein facilitates the cytoplasmic accumulation of the intron-containing viral gag-pol and env mRNAs and is ..
  17. ncbi Rev-dependent association of the intron-containing HIV-1 gag mRNA with the nuclear actin bundles and the inhibition of its nucleocytoplasmic transport by latrunculin-B
    T Kimura
    Departments of Microbiology and Physiology, Kansai Medical University, Moriguchi, Osaka 570 8506, Japan
    Genes Cells 5:289-307. 2000
    A hallmark of HIV-1 gene expression is that unspliced genomic RNA, which also acts as mRNA for the expression of Gag/Pol, is exported to the cytoplasm. Rev directs this transport through the nuclear export signal (NES).
  18. ncbi The HIV-1 Rev trans-activator shuttles between the nucleus and the cytoplasm
    B E Meyer
    Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104 6148
    Genes Dev 8:1538-47. 1994
    The HIV-1 Rev protein is a nuclear trans-activator essential for the transport of unspliced viral transcripts to the cytoplasm...
  19. pmc Perturbation of host nuclear membrane component RanBP2 impairs the nuclear import of human immunodeficiency virus -1 preintegration complex (DNA)
    Ruonan Zhang
    Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina, United States of America
    PLoS ONE 5:e15620. 2010
    ..pore complex and has been implicated in various nucleo-cytoplasmic transport pathways including those for HIV Rev-protein. We sought to investigate the role of RanBP2 in HIV-1 replication...
  20. ncbi Phosphorylation of HIV-1 Rev protein: implication of protein kinase CK2 and pro-directed kinases
    F Meggio
    Dipartimento di Chimica Biologica, Universita di Padova, Italy
    Biochem Biophys Res Commun 226:547-54. 1996
    HIV-1 Rev transactivator is readily phosphorylated at separate regions by protein kinase CK2 and MAP kinase...
  21. ncbi HIV-1 Rev transactivator: a beta-subunit directed substrate and effector of protein kinase CK2
    F Meggio
    Dipartimento di Chimica Biologica, Centro di Studio delle Biomembrane del CNR, Padova, Italy
    Mol Cell Biochem 227:145-51. 2001
    The phosphorylation of HIV-1 Rev by protein kinase CK2 is strictly dependent on the regulatory beta subunit of the kinase and is deeply affected by conformational changes of the substrate outside the phosphorylation site...
  22. pmc Selective translational repression of HIV-1 RNA by Sam68DeltaC occurs by altering PABP1 binding to unspliced viral RNA
    Kim Marsh
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
    Retrovirology 5:97. 2008
    ....
  23. pmc Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actin
    W Hofmann
    Department of Cell and Developmental Biology, Biocenter of the University of Wurzburg, D 97074 Wurzburg, Germany
    J Cell Biol 152:895-910. 2001
    ..Here, we investigate human immunodeficiency virus type 1 (HIV-1) Rev-mediated nuclear export and Mason-Pfizer monkey virus (MPMV) constitutive transport element (CTE)-mediated nuclear ..
  24. ncbi Functional analysis of the interaction of the human immunodeficiency virus type 1 Rev nuclear export signal with its cofactors
    A Kiss
    Institute for Molecular Virology, Saint Louis University School of Medicine, 3681 Park Avenue, Saint Louis, MO 63108, USA
    Virology 314:591-600. 2003
    Human immunodeficiency virus type 1 (HIV-1) Rev-mediated nuclear export of viral RNAs involves the interaction of its leucine-rich nuclear export sequence (NES) with nuclear cofactors...
  25. ncbi The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTP
    P Askjaer
    Department of Molecular and Structural Biology, University of Aarhus, C F Mollers Alle, Building 130, DK 8000 Aarhus C, Denmark
    J Biol Chem 273:33414-22. 1998
    Nuclear export of intron-containing human immunodeficiency virus type 1 (HIV-1) RNA is mediated by the viral Rev protein that contains both an RNA binding domain specific for the viral Rev response element (RRE) and a nuclear export ..
  26. pmc A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus
    F Stutz
    Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
    Mol Cell Biol 16:7144-50. 1996
    The human immunodeficiency virus type 1 Rev protein contains a nuclear export signal (NES) that is required for Rev-mediated RNA export in mammals as well as in the yeast Saccharomyces cerevisiae...
  27. ncbi Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins
    H P Bogerd
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    Cell 82:485-94. 1995
    HIV-1 Rev is the prototype of a class of retroviral regulatory proteins that induce the sequence-specific nuclear export of target RNAs...
  28. pmc HLA class I-driven evolution of human immunodeficiency virus type 1 subtype c proteome: immune escape and viral load
    Christine M Rousseau
    Department of Microbiology, University of Washington, 1959 NE Pacific Street, Box 358070, Seattle, WA 98195 8070, USA
    J Virol 82:6434-46. 2008
    ..to resistant residues among those without the corresponding HLA alleles varied in the order Vpr > Gag > Rev > Pol > Nef > Vif > Tat > Env > Vpu (Fisher's exact test; P < or = 0...
  29. ncbi In vitro interaction between human immunodeficiency virus type 1 Rev protein and splicing factor ASF/SF2-associated protein, p32
    T O Tange
    Department of Molecular Biology, University of Aarhus, C F Møllers Alle, Building 130, DK 8000 Aarhus C, Denmark
    J Biol Chem 271:10066-72. 1996
    Continuous replication of human immunodeficiency virus type 1 requires the expression of the regulatory protein Rev, which binds to the Rev response element (RRE) and up-regulates the cytoplasmic appearance of singly spliced and ..
  30. pmc The carboxy-terminal region of the human immunodeficiency virus type 1 protein Rev has multiple roles in mediating CRM1-related Rev functions
    Yoshiyuki Hakata
    Institute for Genetic Medicine, Hokkaido University, Japan
    J Virol 76:8079-89. 2002
    The human immunodeficiency virus type 1 (HIV-1) regulatory protein, Rev, mediates the nuclear export of unspliced and singly spliced viral mRNAs by bridging viral RNA and export receptor human CRM1 (hCRM1)...
  31. ncbi The cytotoxicity of human immunodeficiency virus type 1 Rev: implications for its interaction with the nucleolar protein B23
    Y Miyazaki
    Institute for Virus Research, Kyoto University, Japan
    Exp Cell Res 219:93-101. 1995
    ..type 1 (HIV-1) encodes several regulatory proteins, including two essential trans-activators for viral replication, Rev and Tat. Both Rev and Tat have a nucleolar targeting signal and are actually located predominantly in the nucleoli...
  32. pmc Sam68 functions in nuclear export and translation of HIV-1 RNA
    Johnny J He
    Center for AIDS Research and Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    RNA Biol 6:384-6. 2009
    ..is mainly controlled at the transcriptional level by HIV-1 Tat and at the post-transcriptional level by HIV-1 Rev. A number of host factors have been identified to be involved in these processes...
  33. ncbi Subcellular localization of human immunodeficiency virus type 1 RNAs, Rev, and the splicing factor SC-35
    S O Bøe
    Department of Microbiology and Immunology, Gade Institute, Bergen High Technology Center, University of Bergen, Norway
    Virology 244:473-82. 1998
    The HIV-1 protein Rev regulates the cytoplasmic levels of incompletely spliced HIV-1 mRNAs. The plasmid pSVc21, which contains a HIV-1 provirus, was introduced into COS cells by transient transfection...
  34. ncbi The post-transcriptional regulator Rev of HIV: implications for its interaction with the nucleolar protein B23
    Y Miyazaki
    Institute for Virus Research, Kyoto University, Japan
    Biochimie 78:1081-6. 1996
    ..HIV-1 encodes several regulatory proteins, including two essential trans-activations for viral replication, Rev and Tat. Both Rev and Tat have a nucleolar targeting signal and are actually located predominantly in the nucleoli...
  35. ncbi Requirement of DDX3 DEAD box RNA helicase for HIV-1 Rev-RRE export function
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    Cell 119:381-92. 2004
    ..export of intron-containing cellular transcripts is restricted in mammalian cells, HIV-1 has evolved the viral Rev protein to overcome this restriction for viral transcripts...
  36. ncbi A DEAD box protein facilitates HIV-1 replication as a cellular co-factor of Rev
    Jianhua Fang
    Division of Infectious Diseases and Environmental Medicine, Department of Medicine, The Dorrance H Hamilton Laboratories, Center for Human Virology and Biodefense, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Virology 330:471-80. 2004
    HIV-1 Rev escorts unspliced viral mRNAs out of the nucleus of infected cells, which allows formation of infectious HIV-1 virions...
  37. ncbi Unique features of HIV-1 Rev protein phosphorylation by protein kinase CK2 ('casein kinase-2')
    O Marin
    Dipartmento di Chimica Biologica and Centro del CNR per lo Studio delle Biomembrane, Università delgi Studi di Padova, Padua, Italy
    FEBS Lett 481:63-7. 2000
    The HIV-1 Rev transactivator is phosphorylated in vitro by protein kinase CK2 at two residues, Ser-5 and Ser-8; these sites are also phosphorylated in vivo...
  38. ncbi Binding of human prothymosin alpha to the leucine-motif/activation domains of HTLV-I Rex and HIV-1 Rev
    S Kubota
    Laboratory of Molecular Virology and Carcinogenesis, NCI Frederick Cancer Research and Development Center, MD 21702, USA
    Eur J Biochem 233:48-54. 1995
    Rex of human T-cell leukemia virus type I (HTLV-I) and Rev of human immunodeficiency virus 1 (HIV-1) are post-transcriptional regulators of viral gene expression...
  39. pmc Identification of a domain in human immunodeficiency virus type 1 rev that is required for functional activity and modulates association with subnuclear compartments containing splicing factor SC35
    D M D'Agostino
    Dipartimento di Scienze Oncologiche e Chirurgiche, Sezione di Oncologia, University of Padova, Italy
    J Virol 74:11899-910. 2000
    The activity of human immunodeficiency virus Rev as a regulator of viral mRNA expression is tightly linked to its ability to shuttle between the nucleus and cytoplasm; these properties are conferred by a leucine-rich nuclear export ..
  40. ncbi High frequency of defective vpu compared with tat and rev genes in brain from patients with HIV type 1-associated dementia
    Elaine R Thomas
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    AIDS Res Hum Retroviruses 23:575-80. 2007
    ..Here we analyzed sequences of tat, rev, and vpu genes in 55 subgenomic clones previously shown to encode functional env genes from brain and lymphoid ..
  41. ncbi Functional domains of the HIV-1 rev gene required for trans-regulation and subcellular localization
    L K Venkatesh
    Institute for Molecular Virology, St Louis University School of Medicine, Missouri 63110
    Virology 176:39-47. 1990
    The rev gene of human immunodeficiency virus type 1 (HIV-1) encodes a 116 amino acid nuclear regulatory protein (Rev) that increases the cytoplasmic expression of viral mRNAs containing the Rev response element (RRE) and coding for the ..
  42. pmc Distinct domains of IkappaB-alpha inhibit human immunodeficiency virus type 1 replication through NF-kappaB and Rev
    B Y Wu
    Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109 0650, USA
    J Virol 71:3161-7. 1997
    ..HIV-1) replication, and unexpectedly, IkappaB-alpha was found both to suppress HIV-1 transcription and to inhibit Rev function. The relative contributions and specificities of these mechanisms to HIV replication were unknown...
  43. pmc Recruitment of the Crm1 nuclear export factor is sufficient to induce cytoplasmic expression of incompletely spliced human immunodeficiency virus mRNAs
    Rui Yi
    Department of Microbiology, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Virol 76:2036-42. 2002
    ..that encode the late, structural proteins of human immunodeficiency virus type 1 (HIV-1) is dependent on the viral Rev regulatory protein...
  44. ncbi The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export
    M Neville
    Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
    Curr Biol 7:767-75. 1997
    The human immunodeficiency virus (HIV-1) uses the viral protein Rev to regulate gene expression by promoting the export of unspliced and partially spliced viral transcripts...
  45. ncbi Nucleolar protein B23 stimulates nuclear import of the HIV-1 Rev protein and NLS-conjugated albumin
    A Szebeni
    Department of Biochemistry, University of Mississippi Medical Center, Jackson 39216, USA
    Biochemistry 36:3941-9. 1997
    ..1994) J. Biol. Chem. 269, 4910-4916] using NLS peptide-conjugated bovine serum albumin (NLS-BSA) or the HIV-1 Rev protein as substrates for import into isolated rat liver nuclei...
  46. ncbi The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs
    U Fischer
    Institut fur Molekularbiologie und Tumorforschung, Marburg, Federal Republic of Germany
    Cell 82:475-83. 1995
    HIV-1 Rev protein directs nuclear export of pre-mRNAs and mRNAs containing its binding site, the Rev response element (RRE)...
  47. pmc Nuclear Factor 90, a cellular dsRNA binding protein inhibits the HIV Rev-export function
    Silvio Urcuqui-Inchima
    Grupo de Inmunovirología, Corporación Biogénesis, Universidad de Antioquia, Medellin, Colombia
    Retrovirology 3:83. 2006
    The HIV Rev protein is known to facilitate export of incompletely spliced and unspliced viral transcripts to the cytoplasm, a necessary step in virus life cycle...
  48. ncbi A Janus splicing regulatory element modulates HIV-1 tat and rev mRNA production by coordination of hnRNP A1 cooperative binding
    Virginie Marchand
    Laboratoire de Maturation des ARN et Enzymologie Moleculaire, UMR 7567 UHP CNRS, Universite Henri Poincare Nancy 1, Boulevard des Aiguillettes, BP239, 54506 Cedex, Vandoeuvre les Nancy, France
    J Mol Biol 323:629-52. 2002
    ..The HIV-1 acceptor site A7 is required for tat and rev mRNA production...
  49. pmc hnRNP E1 and E2 have distinct roles in modulating HIV-1 gene expression
    Kathryn Woolaway
    Department of Molecular and Medical Genetics, University of Toronto Toronto, Ontario, Canada
    Retrovirology 4:28. 2007
    ..We show that hnRNP E1/E2 are able to interact with the ESS3a element of the bipartite ESS in tat/rev exon 3 of HIV-1 and that modulation of hnRNP E1 expression alters HIV-1 structural protein synthesis...
  50. pmc Direct participation of Sam68, the 68-kilodalton Src-associated protein in mitosis, in the CRM1-mediated Rev nuclear export pathway
    Jinliang Li
    Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA
    J Virol 76:8374-82. 2002
    ..efficient nuclear export of incompletely spliced and unspliced HIV-1 mRNA transcripts, which is achieved by Rev expression at an early stage of the viral life cycle...
  51. ncbi Transcriptional and post-transcriptional regulation of HIV-1 gene expression: role of cellular factors for Tat and Rev
    Sergei Nekhai
    Center for Sickle Cell Disease and Department of Biochemistry and Molecular Biolology, Howard University, NW Washington, DC 20059, USA
    Future Microbiol 1:417-26. 2006
    ..This article reviews the interplay between viral proteins Tat and Rev and their cellular cofactors in the transcriptional and post-transcriptional regulation of HIV-1 gene expression...
  52. ncbi Modulation of HIV-1 Rev protein abundance and activity by polyubiquitination with unconventional Lys-33 branching
    Anne Laure Vitte
    Laboratoire de Biologie Moleculaire de la Cellule, UMR5161 CNRS ENS de Lyon, IFR 128 Biosciences Lyon Gerland, 46 allee d Italie, 69364 Lyon Cedex 07, France
    FEBS Lett 580:6155-60. 2006
    The HIV-1 Rev protein plays a key role in virus replication by allowing export to the cytoplasm of unspliced or singly-spliced RNAs. In this report, we investigated whether Rev is modified by ubiquitination or sumoylation...
  53. pmc Nuclear export of the human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev is mediated by its activation domain and is blocked by transdominant negative mutants
    A M Szilvay
    National Centre for Research in Virology, Gade Institute, University of Bergen, Norway
    J Virol 69:3315-23. 1995
    The human immunodeficiency virus type 1 nucleocytoplasmic shuttle protein Rev moves repeatedly between the cytoplasm, a perinuclear zone, the nucleoli, and nucleoplasmic speckles...
  54. ncbi A new nucleoporin-like protein interacts with both HIV-1 Rev nuclear export signal and CRM-1
    G Farjot
    Laboratoire de Virologie, ENS Lyon, Unité INSERM U 412, 46, Allee d Italie, 69364 Lyon Cedex 07, France
    J Biol Chem 274:17309-17. 1999
    The HIV-1 Rev is a shuttling protein required for the nuclear export of unspliced and partially spliced viral mRNA...
  55. pmc Role of polyadenylation in nucleocytoplasmic transport of mRNA
    Y Huang
    Department of Microbiology, University of Connecticut Health Center, Farmington, 06030, USA
    Mol Cell Biol 16:1534-42. 1996
    To examine the role of polyadenylation in the nuclear export of mRNA, we have replaced the poly(A) signal in a Rev-responsive human immunodeficiency virus type 1-based reporter gene with a cis-acting hammerhead ribozyme...
  56. pmc Mechanisms employed by retroviruses to exploit host factors for translational control of a complicated proteome
    Cheryl Bolinger
    Center for Retrovirus Research, Department of Veterinary Biosciences, Molecular, Cellular, and Developmental Biology Graduate Program, The Ohio State University, Columbus, OH, USA
    Retrovirology 6:8. 2009
    ..By contrast, nuclear protein hnRNP E1 negatively modulates HIV-1 Gag, Env, and Rev protein synthesis...
  57. ncbi Multiple importins function as nuclear transport receptors for the Rev protein of human immunodeficiency virus type 1
    Marc Arnold
    Universitat Heidelberg, Abteilung Virologie, Germany
    J Biol Chem 281:20883-90. 2006
    The Rev protein of human immunodeficiency virus type 1 is an RNA-binding protein that is required for nuclear export of unspliced and partially spliced viral mRNAs...
  58. ncbi Interaction of nucleolar protein B23 with peptides related to nuclear localization signals
    A Szebeni
    Department of Biochemistry, University of Mississippi Medical Center, Jackson 39216 4505, USA
    Biochemistry 34:8037-42. 1995
    ..A peptide containing the sequence required for nucleolar localization of the HIV-1 Rev protein had an affinity for B23 approximately 10-fold greater than that of the SV40 T-NLS...
  59. ncbi The road to chromatin - nuclear entry of retroviruses
    Youichi Suzuki
    Laboratory for Host Factors, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University, 53 Shogoin kawara cho, Sakyo ku, Kyoto 606 8507, Japan
    Nat Rev Microbiol 5:187-96. 2007
    ..Before integration into the host genome can occur, this complex must be transported to the nucleus and must cross the nuclear envelope. This Review summarizes our current knowledge of how this journey is accomplished...
  60. ncbi A human nucleoporin-like protein that specifically interacts with HIV Rev
    C C Fritz
    Howard Hughes Medical Institute, Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester 01605, USA
    Nature 376:530-3. 1995
    The Rev protein of human immunodeficiency virus type 1 (HIV-1) facilitates the nuclear export of unspliced and partly spliced viral RNAs...
  61. ncbi Regulation of HIV-1 env mRNA translation by Rev protein
    Celia Perales
    Centro de Biologia Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain
    Biochim Biophys Acta 1743:169-75. 2005
    We have examined the effect of Rev on the regulation of the expression of RRE containing mRNAs when they were synthesised in the nucleus or directly in the cytoplasm...
  62. ncbi Requirement of an additional Sam68 domain for inhibition of human immunodeficiency virus type 1 replication by Sam68 dominant negative mutants lacking the nuclear localization signal
    Jizhong Zhang
    Department of Microbiology and Immunology, Indiana University School of Medicine, R2 302, 950 W Walnut St, Indianapolis, IN 46202, United States
    Gene 363:67-76. 2005
    ..This process is evolutionally made possible by expression of HIV-1 Rev, one of the three HIV-1 proteins encoded by completely spliced HIV-1 RNAs...
  63. pmc Nuclear export of late HIV-1 mRNAs occurs via a cellular protein export pathway
    R A Fridell
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 93:4421-4. 1996
    The Rev protein of HIV-1 is essential for the nuclear export of incompletely spliced viral mRNAs...
  64. ncbi The roles of nucleolar structure and function in the subcellular location of the HIV-1 Rev protein
    M Dundr
    Department of Biochemistry, University of Mississippi Medical Center, Jackson 39216, USA
    J Cell Sci 108:2811-23. 1995
    The human immunodeficiency virus 1 (HIV-1) Rev transactivator protein plays a critical role in the regulation of expression of structural proteins by controlling the pathway of mRNA transport...
  65. pmc Identification of a novel Rev-interacting cellular protein
    Susanne Kramer-Hammerle
    Institute of Molecular Virology, GSF National Research Center for Environment and Health, Ingolstadter Landstrasse 1, D 85764 Neuherberg, Germany
    BMC Cell Biol 6:20. 2005
    ..The HIV Rev protein is a post-transcriptional inducer of HIV gene expression and an important target for interaction with ..
  66. pmc The basic domain of Rev from human immunodeficiency virus type 1 specifically blocks the entry of U4/U6.U5 small nuclear ribonucleoprotein in spliceosome assembly
    J Kjems
    Department of Molecular Biology, University of Aarhus, Denmark
    J Virol 67:4769-76. 1993
    Human immunodeficiency virus type 1 (HIV-1) encodes a regulatory protein, Rev, which is required for cytoplasmic expression of incompletely spliced viral mRNA...
  67. ncbi Functional analysis of backbone cyclic peptides bearing the arm domain of the HIV-1 Rev protein: characterization of the karyophilic properties and inhibition of Rev-induced gene expression
    Elana Hariton-Gazal
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9104, Israel
    Biochemistry 44:11555-66. 2005
    ..the synthesis and activity of a novel backbone cyclic (BC) peptide library based on the sequence of the HIV-1 Rev arginine-rich motif (ARM)...
  68. pmc Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activation
    M Ruhl
    Sandoz Research Institute, Vienna, Austria
    J Cell Biol 123:1309-20. 1993
    ..virus type 1 (HIV-1) structural proteins requires the presence of the viral trans-activator protein Rev. Rev is localized in the nucleus and binds specifically to the Rev response element (RRE) sequence in viral RNA...
  69. pmc RTE and CTE mRNA export elements synergistically increase expression of unstable, Rev-dependent HIV and SIV mRNAs
    Sergey Smulevitch
    Human Retrovirus Pathogenesis Section, National Cancer Institute Frederick, MD 21702 1201, USA
    Retrovirology 3:6. 2006
    ..RTE and CTE are potent in nucleocytoplasmic mRNA transport and expression, neither element is as powerful as the Rev-RRE posttranscriptional control...
  70. ncbi Inhibition of multidrug-resistant HIV-1 by interference with cellular S-adenosylmethionine decarboxylase activity
    Birgit Schäfer
    Heinrich Pette Institute for Experimental Virology and Immunology, Martinistrasse, Hamburg, Germany
    J Infect Dis 194:740-50. 2006
    ..which is a cellular cofactor of the essential human immunodeficiency virus type 1 (HIV-1) regulatory protein Rev. In the present study, we show that SAM486A efficiently suppresses HIV-1 replication, including the replication of ..
  71. ncbi HIV-1 Rev is capable of shuttling between the nucleus and cytoplasm
    N Richard
    Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada
    Virology 204:123-31. 1994
    The mechanism by which Rev facilitates the export, and consequently, the translation of the structural protein mRNAs of the human immunodeficiency virus type 1 remains undefined...
  72. ncbi Evidence for CTL-mediated selection of Tat and Rev mutants after the onset of the asymptomatic period during HIV type 1 infection
    C Guillon
    CNRS, FRE2736 CNRS bioMérieux, IFR128 Biosciences Lyon Gerland, Tour CERVI, 69007 Lyon, France
    AIDS Res Hum Retroviruses 22:1283-92. 2006
    ..We investigated the contribution of both selection processes to the overall evolution of the Tat and Rev regulatory gene sequences from four individuals, ranging in time from just prior to seroconversion to stable ..
  73. ncbi Specific binding of polypyrimidine tract binding protein and hnRNP A1 to HIV-1 CRS elements
    A C Black
    Virus Genes 12:275-85. 1996
    The human immunodeficiency virus (HIV) Rev and human T-cell leukemia virus (HTLV) Rex proteins regulate viral RNA processing...
  74. pmc Sam68 is absolutely required for Rev function and HIV-1 production
    Suhasini Modem
    Department of Immunology and Microbiology, Wayne State University School of Medicine Detroit, MI 48201, USA
    Nucleic Acids Res 33:873-9. 2005
    Sam68 functionally complements for, as well as synergizes with, HIV-1 Rev in Rev response element (RRE)-mediated gene expression and virus production...
  75. ncbi Nuclear pore localization and nucleocytoplasmic transport of eIF-5A: evidence for direct interaction with the export receptor CRM1
    O Rosorius
    Institute for Clinical and Molecular Virology, University Erlangen Nurnberg, Schlossgarten 4, D 91054 Erlangen, Germany
    J Cell Sci 112:2369-80. 1999
    ..The finding that eIF-5A is an essential cofactor of the human immunodeficiency virus type 1 (HIV-1) Rev RNA transport factor suggested that eIF-5A is part of a specific nuclear export pathway...
  76. pmc Integration of HIV-1 DNA is regulated by interplay between viral rev and cellular LEDGF/p75 proteins
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Mol Med 16:34-44. 2010
    The present work describes a novel interaction between the human immunodeficiency virus type 1 (HIV-1) Rev protein and the cellular lens epithelium-derived growth factor p75 (LEDGF/p75) protein in vitro and in virus-infected cells...
  77. doi Over-expression of the HIV-1 Rev promotes death of nondividing eukaryotic cells
    Aviad Levin
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
    Virus Genes 40:341-6. 2010
    Expression of the human immunodeficiency virus type 1 (HIV-1) Rev protein is essential for completion of the viral life cycle...
  78. ncbi Inhibition of HIV replication by dominant negative mutants of Sam68, a functional homolog of HIV-1 Rev
    T R Reddy
    Department of Medicine, University of California San Diego, La Jolla 92093, USA
    Nat Med 5:635-42. 1999
    The HIV-1 Rev protein facilitates the nuclear export of mRNA containing the Rev response element (RRE) through binding to the export receptor CRM-1...
  79. pmc Nucleoporins nup98 and nup214 participate in nuclear export of human immunodeficiency virus type 1 Rev
    A S Zolotukhin
    Human Retrovirus Pathogenesis Group, ABL Basic Research Program, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, Maryland 21702 1201, USA
    J Virol 73:120-7. 1999
    Human immunodeficiency virus type 1 (HIV-1) Rev contains a leucine-rich nuclear export signal that is essential for its nucleocytoplasmic export mediated by hCRM1...
  80. pmc Signal peptide fragments of preprolactin and HIV-1 p-gp160 interact with calmodulin
    B Martoglio
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, Postfach 106249, 69052 Heidelberg, Germany
    EMBO J 16:6636-45. 1997
    ..The functional consequences of such interactions remain to be established. However, our data suggest that signal sequences may be functionally more versatile than anticipated up to now...
  81. pmc Rev inhibition strongly affects intracellular distribution of human immunodeficiency virus type 1 RNAs
    Dusan Cmarko
    Centre of Electron Microscopy, University of Lausanne, 27 Bugnon, CH 1005 Lausanne, Switzerland
    J Virol 76:10473-84. 2002
    ..RNA maturation pathways, we analyzed the intracellular distribution of HIV-1 RNA and the viral regulatory proteins Rev and Tat in transfected COS cells and HIV-1-infected lymphoid C8166 cells by means of ultrastructural in situ ..
  82. ncbi Interaction of eukaryotic initiation factor 5A with the human immunodeficiency virus type 1 Rev response element RNA and U6 snRNA requires deoxyhypusine or hypusine modification
    Y P Liu
    Department of Chemistry, Rutgers State University of New Jersey 08855 0939, USA
    Biol Signals 6:166-74. 1997
    ..Recent studies have suggested that eIF-5A may be the cellular target of the human immunodeficiency virus type 1 Rev and human T cell leukemia virus type 1 Rex proteins...
  83. pmc hnRNP A1 controls HIV-1 mRNA splicing through cooperative binding to intron and exon splicing silencers in the context of a conserved secondary structure
    Christian Kroun Damgaard
    Department of Molecular and Structural Biology, University of Aarhus, DK 8000 Aarhus C, Denmark
    RNA 8:1401-15. 2002
    The removal of the second intron in the HIV-1 rev/tat pre-mRNAs, which involves the joining of splice site SD4 to SA7, is inhibited by hnRNP A1 by a mechanism that requires the intronic splicing silencer (ISS) and the exon splicing ..
  84. ncbi Eukaryotic initiation factor 5A activity and HIV-1 Rev function
    D Bevec
    Novartis Research Institute, Department of Immunodermatology, Vienna, Austria
    Biol Signals 6:124-33. 1997
    ..The unexpected finding that eIF-5A is a cellular cofactor of the HIV-1 Rev trans-activator protein may, however, provide a novel opportunity to reveal precisely what function eIF-5A performs ..
  85. pmc hRIP, a cellular cofactor for Rev function, promotes release of HIV RNAs from the perinuclear region
    Nuria Sánchez-Velar
    University of Massachusetts Medical School, Program in Molecular Medicine and the UMASS Center for AIDS Research CFAR, Worcester, Massachusetts 01605, USA
    Genes Dev 18:23-34. 2004
    Human immunodeficiency virus Rev facilitates the cytoplasmic accumulation of viral RNAs that contain a Rev binding site...
  86. ncbi A role for Rev in the association of HIV-1 gag mRNA with cytoskeletal beta-actin and viral protein expression
    T Kimura
    Department of Microbiology, Kansai Medical University, Osaka, Japan
    Biochimie 78:1075-80. 1996
    Human immunodeficiency virus type-1 (HIV-1) Rev acts by inducing the specific nucleocytoplasmic transport of a class of incompletely spliced RNAs that encodes the viral structural proteins...
  87. pmc Within you, without you: HIV-1 Rev and RNA export
    Andrew I Dayton
    Center for Biologics Evaluation and Research, Food and Drug Administration, USA
    Retrovirology 1:35. 2004
    ..This suggests the ubiquity of RNA helicases in RNA export from the nucleus, and has novel mechanistic implications...
  88. ncbi Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1
    A S Zolotukhin
    Human Retrovirus Pathogenesis Group, NCI Frederick Cancer Research and Development Center, ABL Basic Research Program, Frederick, Maryland 21702 1201, USA
    J Biol Chem 272:11356-60. 1997
    ..is responsible for the cytoplasmic accumulation of RanBP1 and can functionally replace the nuclear export signal of Rev of human immunodeficiency virus type 1...
  89. pmc HIV-1 rev depolymerizes microtubules to form stable bilayered rings
    N R Watts
    Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, MD, USA
    J Cell Biol 150:349-60. 2000
    We describe a novel interaction between HIV-1 Rev and microtubules (MTs) that results in the formation of bilayered rings that are 44-49 nm in external diameter, 3.4-4.2 MD (megadaltons) in mass, and have 28-, 30-, or 32-fold symmetry...
  90. pmc Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in the HIV Rev protein
    Kamyar Hadian
    Institute of Virology, Helmholtz Zentrum Munchen, Research Center for Environmental Health, Ingolstaedter Landstrasse 1, D 85764 Neuherberg, Germany
    J Biol Chem 284:33384-91. 2009
    The Rev protein is a key regulator of human immunodeficiency virus type 1 (HIV-1) gene expression. Rev is primarily known as an adaptor protein for nuclear export of HIV RNAs...
  91. pmc Regulation of human retroviral latency by the NF-kappa B/I kappa B family: inhibition of human immunodeficiency virus replication by I kappa B through a Rev-dependent mechanism
    B Y Wu
    Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109 0650
    Proc Natl Acad Sci U S A 92:1480-4. 1995
    ..I kappa B alpha inhibited HIV replication and gp160 expression by negatively regulating Rev function, most likely acting through a cellular factor involved in Rev transactivation...
  92. doi Stable complex formation between HIV Rev and the nucleosome assembly protein, NAP1, affects Rev function
    Alan Cochrane
    Department of Molecular Genetics, University of Toronto, Toronto, Canada
    Virology 388:103-11. 2009
    The Rev protein of HIV-1 is essential for HIV-1 proliferation due to its role in exporting viral RNA from the nucleus...
  93. ncbi The RNA helicase DDX1 is involved in restricted HIV-1 Rev function in human astrocytes
    Jianhua Fang
    The Dorrance H Hamilton Laboratories, Center for Human Virology and Biodefense, Division of Infectious Diseases and Environmental Medicine, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Virology 336:299-307. 2005
    ..Of note, a low ratio of unspliced versus spliced HIV-1-specific RNA was also investigated, as Rev appeared to act aberrantly in astrocytes, via loss of nuclear and/or nucleolar localization and diminished Rev-..
  94. ncbi Expression of (cac)n/(gtg)n simple repetitive sequences in mRNA of human lymphocytes
    C Epplen
    Ruhr Universitat Bochum, Germany
    Hum Genet 93:35-41. 1994
    ..Longer stretches of perfect simple (cac)n/(gtg)n repeats can rarely be recovered, even from the hnRNA of human lymphocytes...
  95. ncbi Localization of human immunodeficiency virus Rev in transfected and virus-infected cells
    L Luznik
    Department of Medicine, UCSD School of Medicine, La Jolla, California 92093 0665, USA
    AIDS Res Hum Retroviruses 11:795-804. 1995
    The rev gene product of human immunodeficiency virus (HIV) is obligatory for viral replication. Rev interacts specifically with a structured RNA sequence within the viral genome termed the REV response element (RRE)...
  96. ncbi Cytotoxic activity of rev protein of human immunodeficiency virus type 1 by nucleolar dysfunction
    T Nosaka
    Institute for Virus Research, Kyoto University, Japan
    Exp Cell Res 209:89-102. 1993
    The rev protein (Rev) of the human immunodeficiency virus type 1 (HIV-1) is known as a post-transcriptional regulator of viral gene expression. It is located in the cell nucleolus...
  97. ncbi Rev protein suppression of complex formation between nuclear proteins and rev-responsive element-containing RNA of human immunodeficiency virus-1
    A Bek
    Institut fur Physiologische Chemie, Universitat, Mainz, Germany
    Int J Biochem Cell Biol 27:1317-29. 1995
  98. pmc HIV Rev-dependent binding of SF2/ASF to the Rev response element: possible role in Rev-mediated inhibition of HIV RNA splicing
    D M Powell
    Gladstone Institute of Virology and Immunology, University of California, San Francisco 94141 9100, USA
    Proc Natl Acad Sci U S A 94:973-8. 1997
    ..of the structural and enzymatic proteins of type 1 human immunodeficiency virus (HIV-1) is controlled by the rev regulatory gene product...
  99. ncbi The human HIV-1 Rev binding-protein hRIP/Rab (HRB) maps to chromosome 2q36
    T Jones
    Sandoz Research Institute, Vienna, Austria
    Genomics 40:198-9. 1997
  100. ncbi Histographic recording of human immunodeficiency virus type 1 (HIV-1) regulatory protein Rev and nuclear factors
    A Kaneström
    Department of Microbiology and Immunology, Gade Institute, University of Bergen, High Technology Center of Bergen, Norway
    Arch Virol 143:279-94. 1998
    HeLa cells and HeLa cells expressing the HIV-1 regulatory protein Rev were immunostained for Rev and pre-mRNA processing factors and examined histographically by confocal laser scanning microscopy...
  101. ncbi Sam68, RNA helicase A and Tap cooperate in the post-transcriptional regulation of human immunodeficiency virus and type D retroviral mRNA
    T R Reddy
    Department of Medicine, University of California, San Diego, La Jolla 92093 0665, USA
    Oncogene 19:3570-5. 2000
    ..nuclear export pathways for retroviral mRNA have been described: a CRM-1 dependent pathway mediated by the HIV-1 Rev protein and the Rev Response Element (RRE), and a CRM-1 independent pathway mediated by the Constitutive Transport ..

Research Grants64

  1. GFAP as a novel HIV/neuroAIDS biomarker
    Johnny J He; Fiscal Year: 2010
    ....
  2. HIV-1 Preintegration Trafficking and Nuclear Localization
    Alan Engelman; Fiscal Year: 2007
    ..This knowledge in the long run will define novel targets for therapeutic interaction to develop new anti-viral drugs in the fight against HIV/AIDS. ..
  3. MECHANISMS OF HIV-1 ENTRY AND PATHOGENESIS IN ASTROCYTES
    Johnny He; Fiscal Year: 2007
    ..They should also aid in the development of strategies for treating neurologic symptoms of AIDS patients. ..
  4. Tat Interaction with LRP and Its Role in AIDS Dementia
    Johnny He; Fiscal Year: 2006
    ..In addition, these studies will also yield new clues for developing anti-HIV therapeutic strategies for treating HIV-associated neurological disorders occurring in the majority of AIDS patients. ..
  5. LEDGF-Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2005
    ..abstract_text> ..
  6. Use a novel Tat transgenic model to develop neuroAIDS therapeutics
    Johnny He; Fiscal Year: 2007
    ..The proposed study is to explore the possibility of using a mouse model to develop, evaluate, and validate therapeutic strategies specifically targeted at these diseases. ..
  7. HIV-1 Preintegration Trafficking and Nuclear Localization
    ALAN N ENGELMAN; Fiscal Year: 2010
    ..This knowledge in the long run will define novel targets for therapeutic interaction to develop new anti-viral drugs in the fight against HIV/AIDS. ..
  8. Sam68 and HIV-1 replication control in astrocytes
    Johnny J He; Fiscal Year: 2010
    ..This proposal is based on our three major findings (1) Sam68 is an essential cellular co-factor for HIV-1 Rev nuclear export;(2) Inhibition of HIV-1 replication by Sam68 mutants lacking a nuclear localization signal ..
  9. Sam68 and HIV-1 replication control in astrocytes
    Johnny He; Fiscal Year: 2009
    ..This proposal is based on our three major findings (1) Sam68 is an essential cellular co-factor for HIV-1 Rev nuclear export; (2) Inhibition of HIV-1 replication by Sam68 mutants lacking a nuclear localization signal ..
  10. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2009
    ..abstract_text> ..
  11. DELETION MUTAGENESIS WITHIN NONCODING SEGMENTS OF HIV1
    Mark Wainberg; Fiscal Year: 2001
    ..6. To determine whether these attenuated SIV constructs may protect against subsequent challenge with virulent forms of SIV and SIV/HIV hybrids. ..
  12. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 2001
    ..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
  13. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 1999
    ..C. Examination of higher order protein-protein and protein-DNA interactions important for preintegration complex activity. D. Develop an assay for authentic integration of exogenous HIV-1 DNA. ..
  14. MECHANISMS OF HIV-1 ENTRY AND PATHOGENESIS IN ASTROCYTES
    Johnny He; Fiscal Year: 2003
    ..These studies will yield insights that are fundamental to understanding the role of astrocytes in HIV-1 induced CNS pathology and will also elucidate the potential of HIV-1 Nef as targets for therapeutic intervention. ..
  15. Nuclear Localization of HIV-1 Preintegration Complexes
    Alan Engelman; Fiscal Year: 2003
    ..abstract_text> ..
  16. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2004
    ..The results of these experiments will be used to formulate a detailed model of the structural and functional organization of HIV-1 PICs, which will aid the design of antiviral drugs targeted against HIV-1 integration. ..
  17. The Nuclear Pore Complex: Interphase and Mitotic Function
    Katharine S Ullman; Fiscal Year: 2010
    ..Such defects in morphology and DNA content are hallmarks of aggressive cancer cells, and the research proposed here will contribute to a better understanding of how these alterations arise. ..
  18. The Nuclear Pore Complex: Interphase and Mitotic Function
    KATHARINE ULLMAN; Fiscal Year: 2007
    ..The information gathered will impact practical applications, such as optimizing expression of exogenously introduced genes as well as understanding -and ultimately controlling-growth deregulation found in cancerous cells. ..
  19. MOLECULAR MECHANISMS OF NUCLEAR EXPORT
    KATHARINE ULLMAN; Fiscal Year: 2004
    ..Through genetic and biochemical interaction analyses, Nup153 will be used to construct a bigger picture of key connections made between export machinery components. ..