Ran

Summary

Gene Symbol: Ran
Description: RAN, member RAS oncogene family
Alias: ARA24, Gsp1, TC4, GTP-binding nuclear protein Ran, GTPase Ran, OK/SW-cl.81, RanGTPase, androgen receptor-associated protein 24, guanosine triphosphatase Ran, member RAS oncogene family, ras-like protein TC4, ras-related nuclear protein
Species: human

Top Publications

  1. pmc Activation of the Ran GTPase is subject to growth factor regulation and can give rise to cellular transformation
    Thi K Ly
    Department of Molecular Medicine, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 285:5815-26. 2010
  2. ncbi Mechanism of the stress-induced collapse of the Ran distribution
    Yoshinari Yasuda
    Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    Exp Cell Res 312:512-20. 2006
  3. ncbi Serine/threonine kinase Mirk/Dyrk1B is an inhibitor of epithelial cell migration and is negatively regulated by the Met adaptor Ran-binding protein M
    Yonglong Zou
    Pathology Department, Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 278:49573-81. 2003
  4. ncbi Downregulation of the small GTPase ras-related nuclear protein accelerates cellular ageing
    Masahiro Nagai
    Biomolecular Dynamics Laboratory, Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    Biochim Biophys Acta 1830:2813-9. 2013
  5. pmc Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes
    Theis Stüven
    ZMBH, INF 282, D 69120 Heidelberg and Universität Lübeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    EMBO J 22:5928-40. 2003
  6. ncbi The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2)
    T L Bullock
    MRC Laboratory of Molecular Biology, Cambridge, UK
    J Mol Biol 260:422-31. 1996
  7. pmc Importin 13: a novel mediator of nuclear import and export
    J M Mingot
    ZMBH, INF 282, D 69120 Heidelberg, Germany
    EMBO J 20:3685-94. 2001
  8. pmc RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import
    N C Chi
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Cell Biol 135:559-69. 1996
  9. pmc Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex
    U Kutay
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, Germany
    EMBO J 16:1153-63. 1997
  10. ncbi RanGAP mediates GTP hydrolysis without an arginine finger
    Michael J Seewald
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Dortmund, Germany
    Nature 415:662-6. 2002

Research Grants

  1. SHAOCHUNG VICTOR HSIA; Fiscal Year: 2016
  2. GENETIC MODEL OF ENDURANCE EXERCISE IN RATS
    STEVEN BRITTON; Fiscal Year: 1999
  3. MOLECULAR MECHANISMS OF NUCLEAR PROTEIN IMPORT
    Mary Moore; Fiscal Year: 1999
  4. Sphingolipid Signaling in Endothelial Function
    Menq Jer Lee; Fiscal Year: 2012
  5. Nuclear Membrane Fusion in Xenopus Egg Extracts
    Martin W Hetzer; Fiscal Year: 2010
  6. Novel Cilia Trafficking Mechanisms
    BENJAMIN L MARGOLIS; Fiscal Year: 2010
  7. Kristen J Verhey; Fiscal Year: 2016
  8. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2010
  9. Using Chemical Biology to Study the Small GTPase Ra(RMI)
    Karsten Weis; Fiscal Year: 2005
  10. CYTOPLASMIC FACTORS IN NUCLEAR PROTEIN IMPORT
    Stephen Adam; Fiscal Year: 2000

Detail Information

Publications228 found, 100 shown here

  1. pmc Activation of the Ran GTPase is subject to growth factor regulation and can give rise to cellular transformation
    Thi K Ly
    Department of Molecular Medicine, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 285:5815-26. 2010
    Although the small GTPase Ran is best known for its roles in nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope formation, recent studies have demonstrated the overexpression of Ran in multiple tumor types and ..
  2. ncbi Mechanism of the stress-induced collapse of the Ran distribution
    Yoshinari Yasuda
    Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    Exp Cell Res 312:512-20. 2006
    The small GTPase Ran plays a central role in several key nuclear functions, including nucleocytoplasmic transport, cell cycle progression, and assembly of the nuclear envelope...
  3. ncbi Serine/threonine kinase Mirk/Dyrk1B is an inhibitor of epithelial cell migration and is negatively regulated by the Met adaptor Ran-binding protein M
    Yonglong Zou
    Pathology Department, Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 278:49573-81. 2003
    ..In the current study, the Met adaptor protein Ran-binding protein M (RanBPM) was identified as a Mirk-binding protein by yeast two-hybrid analysis...
  4. ncbi Downregulation of the small GTPase ras-related nuclear protein accelerates cellular ageing
    Masahiro Nagai
    Biomolecular Dynamics Laboratory, Department of Frontier Biosciences, Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamada oka, Suita, Osaka 565 0871, Japan
    Biochim Biophys Acta 1830:2813-9. 2013
    The small GTPase Ran, Ras-related nuclear protein, plays important roles in multiple fundamental cellular functions such as nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope formation, by binding to either GTP or ..
  5. pmc Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes
    Theis Stüven
    ZMBH, INF 282, D 69120 Heidelberg and Universität Lübeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    EMBO J 22:5928-40. 2003
    ..In contrast to a previous report, we found no indications of a major and direct role for CRM1 in actin export from mammalian or insect nuclei...
  6. ncbi The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2)
    T L Bullock
    MRC Laboratory of Molecular Biology, Cambridge, UK
    J Mol Biol 260:422-31. 1996
    ..factor 2 (NTF2) facilitates protein transport into the nucleus and interacts with both the small Ras-like GTPase Ran and nucleoporin p62. We have determined the structure of bacterially expressed rat NTF2 at 1...
  7. pmc Importin 13: a novel mediator of nuclear import and export
    J M Mingot
    ZMBH, INF 282, D 69120 Heidelberg, Germany
    EMBO J 20:3685-94. 2001
    Importin beta-related receptors mediate translocation through nuclear pore complexes. Co-operation with the RanGTPase system allows them to bind and subsequently release their substrates on opposite sides of the nuclear envelope, which ..
  8. pmc RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import
    N C Chi
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Cell Biol 135:559-69. 1996
    ..identified that reconstitute nuclear protein import in a permeabilized cell assay: the NLS receptor, p97, and Ran/TC4. Ran/TC4, in turn, interacts with a number of proteins that are involved in the regulation of GTP hydrolysis or are ..
  9. pmc Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex
    U Kutay
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, Germany
    EMBO J 16:1153-63. 1997
    ..Here, we analyse the sites of interaction on importin-beta for its multiple partners. Ran and importin-alpha respectively require residues 1-364 and 331-876 of importin-beta for binding...
  10. ncbi RanGAP mediates GTP hydrolysis without an arginine finger
    Michael J Seewald
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Dortmund, Germany
    Nature 415:662-6. 2002
    ..This has been proposed as a universal mechanism for GAP-mediated GTP hydrolysis. Ran is a nuclear Ras-related protein that regulates both transport between the nucleus and cytoplasm during interphase, ..
  11. pmc Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein export
    M E Lindsay
    Center for Cell Signaling, University of Virginia School of Medicine, Charlottesville, Virginia 22098, USA
    J Cell Biol 153:1391-402. 2001
    ..receptors and mediates the export of proteins from the nucleus by forming a ternary complex with cargo and Ran:GTP. This complex translocates through the nuclear pores and dissociates in the cytosol...
  12. pmc Sequence and characterization of cytoplasmic nuclear protein import factor p97
    N C Chi
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Cell Biol 130:265-74. 1995
    ..cDNA cloning of p97 shows that it is a unique protein containing 23 cysteine residues. Recombinant p97 binds zinc and a bound metal ion is required for the nuclear envelope binding activity of the protein...
  13. pmc A monoclonal antibody to the COOH-terminal acidic portion of Ran inhibits both the recycling of Ran and nuclear protein import in living cells
    M Hieda
    Department of Anatomy and Cell Biology, Osaka University Medical School, Suita, Osaka 565 0871, Japan
    J Cell Biol 144:645-55. 1999
    A small GTPase Ran is a key regulator for active nuclear transport. In immunoblotting analysis, a monoclonal antibody against recombinant human Ran, designated ARAN1, was found to recognize an epitope in the COOH-terminal domain of Ran...
  14. ncbi Targeting of RCC1 to chromosomes is required for proper mitotic spindle assembly in human cells
    William Moore
    Biomedical Research Centre, University of Dundee, Level 5, Ninewells Hospital and Medical School, Dundee DD1 9SY, Scotland, United Kingdom
    Curr Biol 12:1442-7. 2002
    b>Ran GTPase is involved in several aspects of nuclear structure and function, including nucleocytoplasmic transport and nuclear envelope formation...
  15. pmc Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide
    F R Bischoff
    Project Molecular Biology of Mitosis, Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany
    Proc Natl Acad Sci U S A 88:10830-4. 1991
    ..We designate this polypeptide Ran, for ras-related nuclear protein.
  16. pmc Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line
    G T Drivas
    Department of Biochemistry, New York University School of Medicine, New York 10016
    Mol Cell Biol 10:1793-8. 1990
    ..Homologs of the four genes were found in genomic DNA from a variety of mammals and from chicken. The genes were transcriptionally active in a range of human cell types...
  17. ncbi A giant nucleopore protein that binds Ran/TC4
    N Yokoyama
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan
    Nature 376:184-8. 1995
    b>Ran/TC4 is a small nuclear G protein that forms a complex with the chromatin-bound guanine nucleotide release factor RCC1 (ref. 2). Loss of RCC1 causes defects in cell cycle progression, RNA export and nuclear protein import...
  18. ncbi RNA1 encodes a GTPase-activating protein specific for Gsp1p, the Ran/TC4 homologue of Saccharomyces cerevisiae
    J Becker
    Max Planck Institut fur molekulare Physiologie, Abteilung Strukturelle Biologie, Dortmund, Germany
    J Biol Chem 270:11860-5. 1995
    ..In Saccharomyces cerevisiae a gene highly homologous to Ran/TC4 has been identified and named GSP1. Like all ras-related GTP-binding proteins, Gsp1p undergoes cycles of GTP hydrolysis and GDP/GTP exchange...
  19. pmc Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport
    F R Bischoff
    Division of Molecular Biology of Mitosis, German Cancer Research Center, Heidelberg
    Proc Natl Acad Sci U S A 92:1749-53. 1995
    ..pombe. Both this protein and recombinant Rna1p were found to stimulate RanGTPase activity to an extent almost identical to that of human RanGAP1, indicating the functional significance of the ..
  20. pmc Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1
    F R Bischoff
    Division for Molecular Biology of Mitosis, German Cancer Research Center, Heidelberg
    EMBO J 14:705-15. 1995
    ..The purified soluble 23 kDa Ran binding protein RanBP1 does not activate RanGTPase, but increases GTP hydrolysis induced by the RanGTPase-activating protein RanGAP1 by an order of magnitude...
  21. pmc RanGAP1 induces GTPase activity of nuclear Ras-related Ran
    F R Bischoff
    Division for Molecular Biology of Mitosis, German Cancer Research Center, Heidelberg, Federal Republic of Germany
    Proc Natl Acad Sci U S A 91:2587-91. 1994
    The nuclear Ras-related protein Ran binds guanine nucleotide and is involved in cell cycle regulation. Models of the signal pathway predict Ran to be active as Ran...
  22. ncbi Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import
    K M Lounsbury
    Department of Pathology and Comprehensive Cancer Center, University of Vermont, Burlington 05405, USA
    J Biol Chem 271:2357-60. 1996
    ..Components necessary for translocation include the Ran/TC4 GTPase and NTF2/B-2. The functions of these factors at a molecular level remain unclear...
  23. pmc Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta
    J Moroianu
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 93:7059-62. 1996
    ..The small GTPase Ran and the Ran interactive protein, p10, function in the release of the docked complex...
  24. pmc Identification of different roles for RanGDP and RanGTP in nuclear protein import
    D Gorlich
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, Germany
    EMBO J 15:5584-94. 1996
    The importin-alpha/beta heterodimer and the GTPase Ran play key roles in nuclear protein import. Importin binds the nuclear localization signal (NLS)...
  25. pmc A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex
    M J Matunis
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA
    J Cell Biol 135:1457-70. 1996
    b>Ran is a nuclear Ras-like GTPase that is required for the bidirectional transport of proteins and ribnucleoproteins across the nuclear pore complex (NPC)...
  26. ncbi Different binding domains for Ran-GTP and Ran-GDP/RanBP1 on nuclear import factor p97
    N C Chi
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Biol Chem 272:6818-22. 1997
    ..to the nucleus, including the nuclear location sequence receptor (NLS receptor), p97, the small nuclear GTPase Ran/TC4, and several nucleoporins...
  27. pmc A novel class of RanGTP binding proteins
    D Gorlich
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, 69120 Heidelberg, Germany
    J Cell Biol 138:65-80. 1997
    The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal...
  28. ncbi Dynamic and equilibrium studies on the interaction of Ran with its effector, RanBP1
    J Kuhlmann
    Max Planck Institut fur molekulare Physiologie, Dortmund, Germany
    Biochemistry 36:12027-35. 1997
    b>Ran, a small nuclear GTP-binding protein, is one of the most abundant Ras-related proteins in eucaryotic cells. Ran is essential for nucleo-cytoplasmatic transport and is primarily localized in the nucleus and at the nuclear pore complex...
  29. ncbi CRM1 is an export receptor for leucine-rich nuclear export signals
    M Fornerod
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 90:1051-60. 1997
    ..We conclude that CRM1 is an export receptor for leucine-rich nuclear export signals and discuss a model for the role of RanGTP in CRM1 function and in nuclear export in general...
  30. ncbi Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor
    U Kutay
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, Federal Republic of Germany
    Cell 90:1061-71. 1997
    ..Importin alpha is released from this complex in the cytoplasm by the combined action of RanBP1 and RanGAP1. CAS binds preferentially to NLS-free importin alpha, explaining why import substrates stay in the nucleus...
  31. ncbi Human RanBP3, a group of nuclear RanGTP binding proteins
    L Mueller
    Division for Molecular Biology of Mitosis, German Cancer Research Center, Heidelberg
    FEBS Lett 427:330-6. 1998
    A group of novel human Ran-binding proteins, RanBP3, was identified using the yeast two-hybrid system via Ran-mediated interaction with the nucleotide exchange factor RCC1...
  32. ncbi Identification of a tRNA-specific nuclear export receptor
    U Kutay
    Zentrum fur Molekulare Biologie, Universitat Heidelberg, Federal Republic of Germany
    Mol Cell 1:359-69. 1998
    ..RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm...
  33. pmc NTF2 mediates nuclear import of Ran
    K Ribbeck
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    EMBO J 17:6587-98. 1998
    ..We describe here the development of an in vitro system to study how nuclear Ran is replenished...
  34. ncbi The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTP
    P Askjaer
    Department of Molecular and Structural Biology, University of Aarhus, C F Mollers Alle, Building 130, DK 8000 Aarhus C, Denmark
    J Biol Chem 273:33414-22. 1998
    ..export receptor for proteins carrying a Rev-like NES in a process that also requires the GTP bound form of the Ran GTPase...
  35. pmc CRM1-mediated recycling of snurportin 1 to the cytoplasm
    E Paraskeva
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 145:255-64. 1999
    ..This mechanism appears crucial for productive import cycles as it can ensure that CRM1 only exports snurportin 1 that has already released its import substrate in the nucleus...
  36. pmc The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human
    J Katahira
    BZH, Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    EMBO J 18:2593-609. 1999
    ..Thus, the human TAP-p15 complex can functionally replace the Mex67p-Mtr2p complex in yeast and thus performs a conserved role in nuclear mRNA export...
  37. ncbi Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp
    Y M Chook
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    Nature 399:230-7. 1999
    ..In the nucleus, Ran x GTP binds karyopherin-beta2 and dissociates the substrate. Here we present the 3...
  38. ncbi Structural view of the Ran-Importin beta interaction at 2.3 A resolution
    I R Vetter
    Max Planck Institut fur molekulare Physiologie, Dortmund, Germany
    Cell 97:635-46. 1999
    ..They interact specifically with the GTP-binding protein Ran, which in turn regulates their interaction with cargo...
  39. pmc Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export
    B E Black
    Center for Cell Signaling, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 19:8616-24. 1999
    ..Assembly and disassembly reactions of receptor-substrate complexes are coordinated by Ran, a GTP-binding protein whose nucleotide state is regulated catalytically by effector proteins...
  40. pmc Exportin 4: a mediator of a novel nuclear export pathway in higher eukaryotes
    G Lipowsky
    ZMBH, 69120 Heidelberg, Germany
    EMBO J 19:4362-71. 2000
    ..translocation through nuclear pore complexes, shuttle between nucleus and cytoplasm and co-operate with the RanGTPase system to regulate their interactions with cargo molecules in a compartment-specific manner...
  41. ncbi Identification of two novel RanGTP-binding proteins belonging to the importin beta superfamily
    U Kutay
    Swiss Federal Institute of Technology Zurich, Institute of Biochemistry, 8092 Zurich, Switzerland
    J Biol Chem 275:40163-8. 2000
    ..new members of the importin beta family, we used affinity chromatography on immobilized RanGTP and isolated Ran-binding protein (RanBP) 16 from HeLa cell extracts...
  42. pmc Importin-11, a nuclear import receptor for the ubiquitin-conjugating enzyme, UbcM2
    S M Plafker
    Center for Cell Signaling, Box 800577, HSC and Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA
    EMBO J 19:5502-13. 2000
    ..Importin-11 interacts with the Ran GTPase, and constitutively shuttles between the nuclear and cytoplasmic compartments...
  43. ncbi Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1)
    L Renault
    Max Planck Institut fur molekulare Physiologie, Postfach 50 02 47, 44202, Dortmund, Germany
    Cell 105:245-55. 2001
    ..chromatin-bound protein, is the guanine nucleotide exchange factor (GEF) for the nuclear GTP binding protein Ran. We report here the 1...
  44. pmc RanBP3 influences interactions between CRM1 and its nuclear protein export substrates
    L Englmeier
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO Rep 2:926-32. 2001
    We investigated the role of RanBP3, a nuclear member of the Ran-binding protein 1 family, in CRM1-mediated protein export in higher eukaryotes. RanBP3 interacts directly with CRM1 and also forms a trimeric complex with CRM1 and RanGTP...
  45. ncbi Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor
    Michael E Nemergut
    Department of Microbiology, The Center for Cell Signaling, The University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:17385-8. 2002
    b>Ran-binding protein 3 (RanBP3) is an approximately 55-kDa protein that functions as a cofactor for Crm1-mediated nuclear export. RanBP3 stimulates export by enhancing the affinity of Crm1 for Ran.GTP and cargo...
  46. pmc The carboxy-terminal region of the human immunodeficiency virus type 1 protein Rev has multiple roles in mediating CRM1-related Rev functions
    Yoshiyuki Hakata
    Institute for Genetic Medicine, Hokkaido University, Japan
    J Virol 76:8079-89. 2002
    ..are a basis of complex formation, can be initiated without cellular factors and are subsequently enhanced by hCRM1-Ran-GTP...
  47. ncbi Ran modulates spindle assembly by regulating a subset of TPX2 and Kid activities including Aurora A activation
    Nadia Trieselmann
    Department of Medical Genetics and Microbiology, University of Toronto, 1 King s College Circle, Toronto, Ontario, Canada, M5S 1A8
    J Cell Sci 116:4791-8. 2003
    b>Ran, a GTPase in the Ras superfamily, is proposed to be a spatial regulator of microtubule spindle assembly by maintaining key spindle assembly factors in an active state close to chromatin...
  48. pmc A multifunctional domain in human CRM1 (exportin 1) mediates RanBP3 binding and multimerization of human T-cell leukemia virus type 1 Rex protein
    Yoshiyuki Hakata
    Institute for Genetic Medicine, Hokkaido University, Kita ku, Sapporo 060 0815, Japan
    Mol Cell Biol 23:8751-61. 2003
    ..Our results provide the molecular basis underlying the species-specific ability of HTLV-1 to propagate in human cells...
  49. pmc Contribution of noncentrosomal microtubules to spindle assembly in Drosophila spermatocytes
    Elena Rebollo
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS Biol 2:E8. 2004
    ..These observations are consistent with a model in which centrosomal and noncentrosomal microtubules contribute to the assembly and are required for the robustness of the cell division spindle in cells that contain centrosomes...
  50. pmc Phosphorylation of RCC1 in mitosis is essential for producing a high RanGTP concentration on chromosomes and for spindle assembly in mammalian cells
    Hoi Yeung Li
    Department of Embryology, Howard Hughes Medical Institute, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    Genes Dev 18:512-27. 2004
    Spindle assembly is subject to the regulatory controls of both the cell-cycle machinery and the Ran-signaling pathway...
  51. ncbi Architecture of CRM1/Exportin1 suggests how cooperativity is achieved during formation of a nuclear export complex
    Carlo Petosa
    European Molecular Biology Laboratory, Grenoble Outstation, B P 181, 38042 Grenoble Cedex 9, France
    Mol Cell 16:761-75. 2004
    ..export signal (NES) by forming a cooperative ternary complex with the NES-bearing substrate and the small GTPase Ran. We present a structural model of human CRM1 based on a combination of X-ray crystallography, homology modeling, ..
  52. ncbi Tissue array analysis of expression microarray candidates identifies markers associated with tumor grade and outcome in serous epithelial ovarian cancer
    Veronique Ouellet
    Centre de Recherche du Centre Hospitalier de l Université de Montréal Institut du cancer de Montréal, Montreal, Canada
    Int J Cancer 119:599-607. 2006
    ..this study, we used immunohistochemistry to monitor a subset of differently expressed candidates (Ahr, Paep, Madh3, Ran, Met, Mek1, Ccne1, Ccd20, Cks1 and Cas)...
  53. pmc RanBP10 is a cytoplasmic guanine nucleotide exchange factor that modulates noncentrosomal microtubules
    Harald Schulze
    Dana Farber Cancer Institute, Boston, MA 02115, USA
    J Biol Chem 283:14109-19. 2008
    Microtubule spindle assembly in mitosis is stimulated by Ran.GTP, which is generated along condensed chromosomes by the guanine nucleotide exchange factor (GEF) RCC1...
  54. doi Ran suppresses paclitaxel-induced apoptosis in human glioblastoma cells
    Im Sun Woo
    Department of Pharmacology, Gyeongsang Institute of Health Science, Gyeongsang National University School of Medicine, Jinju 660 751, South Korea
    Apoptosis 13:1223-31. 2008
    Yeast-based functional screening of a human glioblastoma cDNA library identified ras-related nuclear protein (Ran) as a novel suppressor of Bcl-2-associated X protein (Bax), a pro-apoptotic member of the Bcl-2 family of proteins...
  55. doi Crystal structure of the nuclear export receptor CRM1 in complex with Snurportin1 and RanGTP
    Thomas Monecke
    Abteilung für Molekulare Strukturbiologie, Institut fur Mikrobiologie und Genetik, GZMB, Georg August Universitat Gottingen, Justus von Liebig Weg 11, 37077 Gottingen, Germany
    Science 324:1087-91. 2009
    ..5 angstrom resolution (where SPN1 is snurportin1 and RanGTP is guanosine 5' triphosphate-bound Ran)...
  56. pmc Genetic variation in MicroRNA genes and risk of oral premalignant lesions
    Jessica Clague
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Mol Carcinog 49:183-9. 2010
    ....
  57. pmc Genetic variations in microRNA-related genes are associated with survival and recurrence in patients with renal cell carcinoma
    Jie Lin
    Department of Epidemiology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Carcinogenesis 31:1805-12. 2010
    ..Future investigation in larger populations and functional characterizations are necessary to validate these results...
  58. pmc An essential role for Ran GTPase in epithelial ovarian cancer cell survival
    Véronique Barrès
    Centre de Recherche du Centre Hospitalier de l Université de Montréal Institut du cancer de Montréal, 1560 Sherbrooke Est, Montreal H2L 4M1, Canada
    Mol Cancer 9:272. 2010
    We previously identified that Ran protein, a member of the Ras GTPase family, is highly expressed in high grade and high stage serous epithelial ovarian cancers, and that its overexpression is associated with a poor prognosis...
  59. pmc The defective nuclear lamina in Hutchinson-gilford progeria syndrome disrupts the nucleocytoplasmic Ran gradient and inhibits nuclear localization of Ubc9
    Joshua B Kelley
    Center for Cell Signaling, University of Virginia, Charlottesville, Virginia, USA
    Mol Cell Biol 31:3378-95. 2011
    ..We found that progerin reduces the nuclear/cytoplasmic concentration of the Ran GTPase and inhibits the nuclear localization of Ubc9, the sole E2 for SUMOylation, and of TPR, the nucleoporin that ..
  60. pmc Loading and unloading: orchestrating centrosome duplication and spindle assembly by Ran/Crm1
    Anuradha S Budhu
    Laboratory of Human Carcinogenesis, Center for Cancer Research, NCI, NIH, Bethesda, Maryland 20892 4255, USA
    Cell Cycle 4:1510-4. 2005
    ..Recent studies suggest that the Ran/Crm1 complex not only regulates nucleocytoplasmic transport but is also independently involved in mitotic spindle ..
  61. ncbi Analysis of a RanGTP-regulated gradient in mitotic somatic cells
    Petr Kalab
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3200, USA
    Nature 440:697-701. 2006
    The RanGTPase cycle provides directionality to nucleocytoplasmic transport, regulating interactions between cargoes and nuclear transport receptors of the importin-beta family...
  62. ncbi Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope
    D Gorlich
    Wellcome CRC Institute, Cambridge, UK
    Curr Biol 5:383-92. 1995
    ..A 60 kD protein, importin, is essential for the first nuclear import step, and the small G protein Ran/TC4 is essential for the second...
  63. ncbi RanBP1 is crucial for the release of RanGTP from importin beta-related nuclear transport factors
    F R Bischoff
    Abteilung Molekulare Biologie der Mitose, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    FEBS Lett 419:249-54. 1997
    ..The transiently released RanGTP x RanBP1 complex is then induced by RanGAP to hydrolyse GTP, preventing the receptor to rebind RanGTP. The efficient release of importin beta from RanGTP requires importin alpha, in addition to RanBP1...
  64. ncbi Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation
    R E Carazo-Salas
    EMBL, Heidelberg, Germany
    Nature 400:178-81. 1999
    ..RCC1, the guanine-nucleotide-exchange factor for the GTPase protein Ran, is a component of chromatin...
  65. ncbi Polo-like kinase 1-mediated phosphorylation of the GTP-binding protein Ran is important for bipolar spindle formation
    Yang Feng
    Laboratory of Cancer Prevention, NCI at Frederick, Frederick, MD, USA Nanobiology Program, CCR, NCI at Frederick, Frederick, MD, USA
    Biochem Biophys Res Commun 349:144-52. 2006
    ..In this study, we report that the small GTP/GDP-binding protein Ran is associated with Plk1...
  66. pmc Characterization of the human herpesvirus 6 U69 gene product and identification of its nuclear localization signal
    Yuji Isegawa
    Department of Infectious Disease Control, G 5, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
    J Virol 82:710-8. 2008
    ..that the nuclear transport of the U69 protein was mediated by importin alpha/beta in conjunction with the small GTPase Ran. When the import assay was performed with a low concentration of each importin-alpha subtype, NPI2/importin-..
  67. pmc Dynamic localisation of Ran GTPase during the cell cycle
    James R A Hutchins
    Biomedical Research Institute, College of Medicine, Dentistry and Nursing, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK
    BMC Cell Biol 10:66. 2009
    b>Ran GTPase has multiple functions during the cell division cycle, including nucleocytoplasmic transport, mitotic spindle assembly and nuclear envelope formation...
  68. pmc A mutant form of the Ran/TC4 protein disrupts nuclear function in Xenopus laevis egg extracts by inhibiting the RCC1 protein, a regulator of chromosome condensation
    M Dasso
    Laboratory of Molecular Embryology, NICHD, National Institutes of Health, Bethesda, MD 20892 5430
    EMBO J 13:5732-44. 1994
    The Ran protein is a small GTPase that has been implicated in a large number of nuclear processes including transport. RNA processing and cell cycle checkpoint control...
  69. ncbi Model of the ran-RCC1 interaction using biochemical and docking experiments
    Y Azuma
    Department of Molecular Biology, Kyushu University, Fukuoka, Japan
    J Mol Biol 289:1119-30. 1999
    ..condensation, is the guanine nucleotide exchange factor (GEF) for the nuclear Ras-like GTP-binding protein Ran. Its structure was solved by X-ray crystallography and revealed a seven-bladed beta-propeller, one side of which ..
  70. pmc Facilitated nucleocytoplasmic shuttling of the Ran binding protein RanBP1
    K Plafker
    Markey Center for Cell Signaling and Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 20:3510-21. 2000
    The Ran binding protein RanBP1 is localized to the cytosol of interphase cells. A leucine-rich nuclear export signal (NES) near the C terminus of RanBP1 is essential to maintain this distribution...
  71. ncbi Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts
    C Zhang
    Biomedical Research Centre, University of Dundee, Level 5, Ninewells Hospital and Medical School, Dundee DD1 9SY, Scotland, UK
    Science 288:1429-32. 2000
    ..NEs incorporating nuclear pores were assembled around beads coated with the guanosine triphosphatase Ran, forming pseudo-nuclei that actively imported nuclear proteins...
  72. ncbi Chromosomal association of Ran during meiotic and mitotic divisions
    Beth Hinkle
    Department of Physiology, University of Connecticut Health Center, Farmington, CT 06032, USA
    J Cell Sci 115:4685-93. 2002
    Recent studies in Xenopus egg extracts indicate that the small G protein Ran has a central role in spindle assembly and nuclear envelope reformation. We determined Ran localization and dynamics in cells during M phase...
  73. pmc Hyperosmotic stress signaling to the nucleus disrupts the Ran gradient and the production of RanGTP
    Joshua B Kelley
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908, USA
    Mol Biol Cell 18:4365-76. 2007
    The RanGTP gradient depends on nucleocytoplasmic shuttling of Ran and its nucleotide exchange in the nucleus...
  74. doi High expression of Ran GTPase is associated with local invasion and metastasis of human clear cell renal cell carcinoma
    Hideyuki Abe
    Department of Urology, Dokkyo Medical University, Tochigi, Japan
    Int J Cancer 122:2391-7. 2008
    The Ran small GTPase (Ran) is involved in the regulation of nuclear transport, microtubule nucleation and dynamics, and spindle assembly...
  75. pmc Localized RanGTP accumulation promotes microtubule nucleation at kinetochores in somatic mammalian cells
    Liliana Torosantucci
    IBPM Institute of Molecular Biology and Pathology, National Research Council, c o University of Rome La Sapienza, 00185 Rome, Italy
    Mol Biol Cell 19:1873-82. 2008
    ..The complex of RanGTP, the export receptor Crm1, and nuclear export signal-bearing proteins regulates microtubule nucleation at kinetochores...
  76. doi Association of a common AGO1 variant with lung cancer risk: a two-stage case-control study
    Jong Sik Kim
    Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea
    Mol Carcinog 49:913-21. 2010
    ..In stage 1 of the study, 24 SNPs in the 11 miRNA biosynthesis genes (DROSHA, DGCR8, RAN, XPO5, DICER, AGO1, AGO2, HIWI, GEMIN3, GEMIN4, and TRBP) were genotyped in 100 lung cancer patients and 100 ..
  77. doi Reduction of exportin 6 activity leads to actin accumulation via failure of RanGTP restoration and NTF2 sequestration in the nuclei of senescent cells
    Su Hyun Park
    Department of Biochemistry and Molecular Biology, BK21 Division of Cell Transformation and Restoration, Ajou University School of Medicine, Suwon 443 721, Republic of Korea
    Exp Cell Res 317:941-54. 2011
    ..Expression of exportin 6 (Exp6) and small G-protein, Ran, was significantly reduced in the replicative senescence, but not yet in SIPS, whereas nuclear import of actin by ..
  78. pmc Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1
    E Noguchi
    Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan
    EMBO J 15:5595-605. 1996
    ..cerevisiae cDNA encoding a protein homologous to Schizosaccharomyces pombe protein Dis3sp, using as bait, human GTPase Ran. The DIS3 gene is essential for viability and complements S.pombe mutant dis3-54 which is defective in mitosis...
  79. ncbi Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport
    I R Vetter
    Abteilung Strukturelle Biologie, Max Planck Institut fur molekulare Physiologie, Dortmund, Germany
    Nature 398:39-46. 1999
    The protein Ran is a small GTP-binding protein that binds to two types of effector inside the cell: Ran-binding proteins, which have a role in terminating export processes from the nucleus to the cytoplasm, and importin-beta-like ..
  80. pmc Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing
    M Ren
    Department of Cell Biology, New York University Medical Center, New York 10016
    Mol Cell Biol 15:2117-24. 1995
    The small Ras-related GTP binding and hydrolyzing protein Ran has been implicated in a variety of processes, including cell cycle progression, DNA synthesis, RNA processing, and nuclear-cytosolic trafficking of both RNA and proteins...
  81. pmc Significant proportions of nuclear transport proteins with reduced intracellular mobilities resolved by fluorescence correlation spectroscopy
    Allison Paradise
    Department of Molecular Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA
    J Mol Biol 365:50-65. 2007
    ..We analyzed dynamic properties of importin alpha, importin beta, Ran and NTF2 in nucleus, cytoplasm and at the nuclear pore of neuroblastoma cells using fluorescence correlation ..
  82. ncbi Localization of the Ran-GTP binding protein RanBP2 at the cytoplasmic side of the nuclear pore complex
    N Wilken
    Department of Cell and Developmental Biology, Theodor Boveri Institute, University of Wurzburg, Germany
    Eur J Cell Biol 68:211-9. 1995
    A partial cDNA clone coding for the mouse homologue of the human Ran-GTP binding protein, RanBP2, has been isolated by screening of a murine expression library with antibodies to nup180, a previously identified nuclear pore complex ..
  83. doi Regulation of Wnt signaling by the nuclear pore complex
    Miki Shitashige
    Chemotherapy Division and Cancer Proteomics Project, National Cancer Center Research Institute, Tokyo, Japan
    Gastroenterology 134:1961-71, 1971.e1-4. 2008
    ..However, beta-catenin has no nuclear localization signal, and the mechanisms by which beta-catenin is imported into the nucleus and forms a complex with the TCF-4 nuclear protein are poorly understood...
  84. pmc Ran overexpression leads to diminished T cell responses and selectively modulates nuclear levels of c-Jun and c-Fos
    Xiaoying Qiao
    Laboratory of Immunology, Centre de Recherche, Centre Hospitalier de l Universite de Montreal, Montreal, Quebec H2L 4M1, Canada
    J Biol Chem 285:5488-96. 2010
    Ras-related nuclear protein (Ran) is a Ras family GTPase, and its documented functions are the regulation of DNA replication, cell cycle progression, nuclear structure formation, RNA processing and exportation, and nuclear protein ..
  85. ncbi Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region
    J Wu
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021, USA
    J Biol Chem 270:14209-13. 1995
    The Ras-related nuclear protein, Ran, has been implicated in nuclear transport...
  86. ncbi [The Hannover School of Veterinary Medicine in the Third Reich. Part 1: early history, assumption of power and consolidation of the Nazi regime]
    M Schimanski
    ,
    Dtsch Tierarztl Wochenschr 108:380-5. 2001
    ..After the assumption of power by Hitler the 'Gleichschaltung'--which also took place at the universities--ran mostly smoothly at the veterinary school...
  87. pmc Yeast heat shock mRNAs are exported through a distinct pathway defined by Rip1p
    C A Saavedra
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Genes Dev 11:2845-56. 1997
    ..encoding Hsp70 proteins Ssa1p and Ssa4p) are efficiently exported in a process that is independent of the small GTPase Ran/Gsp1p, which is essential for most nucleocytoplasmic transport...
  88. ncbi A single point mutation at the 3'-untranslated region of Ran mRNA leads to profound changes in lipopolysaccharide endotoxin-mediated responses
    P M Wong
    Department of Pathology and Laboratory Medicine, Fels Institute, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Biol Chem 276:33129-38. 2001
    By functional cDNA expression cloning, we have previously established that Ran is important in lipopolysaccharide (LPS) signaling...
  89. ncbi Structural basis for the nuclear protein import cycle
    M Stewart
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Biochem Soc Trans 34:701-4. 2006
    ..In the nucleus, Ran-GTP binds to importin-beta, dissociating the import complex...
  90. pmc Overexpression of OsRAN2 in rice and Arabidopsis renders transgenic plants hypersensitive to salinity and osmotic stress
    Aiping Zang
    Institute of Plant Physiology and Ecology, Graduate School of Chinese Academy of Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China
    J Exp Bot 61:777-89. 2010
    ..Ras-related nuclear protein (Ran) GTPase is required for regulating transport of proteins and RNA across the nuclear envelope and also has roles in ..
  91. ncbi Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4
    E Coutavas
    Department of Biochemistry, NYU Medical Center, New York 10016
    Nature 366:585-7. 1993
    The human Ras-related nuclear protein Ran/TC4 (refs 1-4) is the prototype of a well conserved family of GTPases that can regulate both cell-cycle progression and messenger RNA transport...
  92. ncbi Identification of Ras-related nuclear protein, targeting protein for xenopus kinesin-like protein 2, and stearoyl-CoA desaturase 1 as promising cancer targets from an RNAi-based screen
    Susan E Morgan-Lappe
    Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, Illinois 60064, USA
    Cancer Res 67:4390-8. 2007
    ..We found that siRNAs specifically targeting ras-related nuclear protein (Ran), targeting protein for Xenopus kinesin-like protein 2 (TPX2), and stearoyl-CoA desaturase 1 (SCD1), significantly ..
  93. ncbi Characterization of a ras-related nuclear protein (Ran protein) up-regulated in shrimp antiviral immunity
    Fang Han
    Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China
    Fish Shellfish Immunol 23:937-44. 2007
    ..In this investigation, an important signal transduction factor-Ran gene encoding ras-related nuclear protein (Ran protein) was characterized in shrimp...
  94. ncbi Tissue-specific expression of Ran isoforms in the mouse
    E E Coutavas
    Department of Biochemistry, New York University Medical Center, New York 10016
    Mamm Genome 5:623-8. 1994
    b>Ran genes encode a family of well-conserve small nuclear GTPases (Ras-related nuclear proteins), whose function is implicated in both normal cell cycle progression and the transport of RNA and proteins between the nucleus and the ..
  95. ncbi A search for Drosophila neural precursor genes identifies ran
    K Koizumi
    The Neurogenetics Unit, Laboratory of Neurochemistry, NINDS, NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Dev Genes Evol 211:67-75. 2001
    The Drosophila ran gene has been isolated in a differential cDNA screen designed to identify genes that are dynamically expressed in embryonic neuroblasts...
  96. ncbi Perinuclear and nuclear envelope localizations of Arabidopsis Ran proteins
    Lian Ma
    State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
    Plant Cell Rep 26:1373-82. 2007
    ..The plant Ran2 shares high sequence homology, 78 and 86% at the amino acid level, with human Ran/TC4 and C. elegans Ran, respectively...
  97. pmc RCC1 uses a conformationally diverse loop region to interact with the nucleosome: a model for the RCC1-nucleosome complex
    Joseph R England
    Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, 108 Althouse Laboratory, The Pennsylvania State University, University Park, PA 16802 1014, USA
    J Mol Biol 398:518-29. 2010
    ..such as mitosis, nucleocytoplasmic transport, and nuclear envelope formation because RCC1 recruits the small GTPase Ran (Ras-related nuclear protein) to chromatin and sets up a Ran-GTP gradient around the chromosomes...
  98. ncbi Regulation of mRNA export in response to stress in Saccharomyces cerevisiae
    C Saavedra
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    Genes Dev 10:1608-20. 1996
    ..cells carrying temperature-sensitive alleles of RNA1, PRP20, or an inducible dominant-negative allele of GSP1, the S. cerevisiae homolog of RAN/TC4...
  99. pmc Functional analysis of key nuclear trafficking components reveals an atypical Ran network required for parasite pathogenesis
    Matthew B Frankel
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, 1300 University Avenue, Madison, WI 53706, USA
    Mol Microbiol 70:410-20. 2008
    ..RCC1 proteins function as a guanine exchange factor for Ras-related nuclear protein (Ran), an abundant GTPase responsible for the majority of nucleocytoplasmic transport...
  100. doi Reduced Ran expression in Ran(+/-) fibroblasts increases cytokine-stimulated nuclear abundance of the AP-1 subunits c-Fos and c-Jun
    Diep Ngoc Thi Pham
    Laboratory of Immunology, Centre Hospitalier de l Université de Montréal CRCHUM Hôpital Notre Dame, Montreal, Quebec, Canada
    FEBS Lett 584:4623-6. 2010
    b>Ran (Ras-related nuclear protein), a Ras family GTPase, is involved in multiple cellular functions, including the regulation of DNA replication, cell cycle progression, nuclear structure formation, RNA processing-exportation, and nuclear ..
  101. pmc Screening of potential molecular targets for colorectal cancer therapy
    Kimi Honma
    Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan
    Int J Gen Med 2:243-57. 2009
    ..The results indicate that the downregulation of PSMA7 (proteasome subunit, alpha-type, 7) and RAN (ras-related nuclear protein) most efficiently induced apoptosis of HT-29 cells...

Research Grants66

  1. SHAOCHUNG VICTOR HSIA; Fiscal Year: 2016
    ..Aim 3: To test the hypothesis that Regulator of Chromosome Condensation 1 (RCC1) and RAs- related Nuclear protein (Ran) complex participated in the global epigenetic control of HSV-1 transcription/ replication by maintaining ..
  2. GENETIC MODEL OF ENDURANCE EXERCISE IN RATS
    STEVEN BRITTON; Fiscal Year: 1999
    ..The 13 high selected females ran on average 3.55 times further than the 13 low selected females. The 13 high selected males ran 5...
  3. MOLECULAR MECHANISMS OF NUCLEAR PROTEIN IMPORT
    Mary Moore; Fiscal Year: 1999
    ..proteins have been identified that are required to support nuclear protein import in digitonin permeabilized cells: Ran, p10, karyopherin beta, and importin (which is probably equivalent to karyopherin alpha)...
  4. Sphingolipid Signaling in Endothelial Function
    Menq Jer Lee; Fiscal Year: 2012
    ..loop (i3) of S1P1 receptor and the nuclear translocation of S1P1 receptor is mediated by the importin [unreadable]1-Ran nuclear transport machinery...
  5. Nuclear Membrane Fusion in Xenopus Egg Extracts
    Martin W Hetzer; Fiscal Year: 2010
    ..We have generated new information about the two major regulators of nuclear assembly, the GTPase Ran and the AAA-ATPase p97. Our specific aims are: 1...
  6. Novel Cilia Trafficking Mechanisms
    BENJAMIN L MARGOLIS; Fiscal Year: 2010
    ..of our study on Crumbs3, we have identified a novel cilia and centrosomal trafficking mechanism that involves the Ran/Importin system...
  7. Kristen J Verhey; Fiscal Year: 2016
    ..We will determine the mechanisms by which ciliary access of kinesin motors is regulated by importin proteins and a Ran gradient...
  8. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2010
    ..This import pathway, often referred as classical, also requires the small GTPase Ran, which exists mainly bound to GTP in the nucleoplasm and to GDP in the cytoplasm...
  9. Using Chemical Biology to Study the Small GTPase Ra(RMI)
    Karsten Weis; Fiscal Year: 2005
    ..is to develop assays that will be applied to high throughput small molecule screens designed to target the small GTPase Ran and its associated proteins...
  10. CYTOPLASMIC FACTORS IN NUCLEAR PROTEIN IMPORT
    Stephen Adam; Fiscal Year: 2000
    ..Subsequent release of the docked receptor complex and translocation to the nuclear interior requires the small GTPase Ran/TC4...
  11. MECHANISMS OF NUCLEAR IMPORT
    Larry Gerace; Fiscal Year: 2006
    ..One of the critical regulators of import is the small GTPase Ran. The long-term goal of this work is to contribute to a detailed understanding of nucleocytoplasmic transport...
  12. Ran & Energy Requirements for Nuclear Protein Import
    KIMON KANELAKIS; Fiscal Year: 2004
    ..The small GTPase Ran plays a critical role in nuclear import by modulating the interaction of cargo with these transport receptors...
  13. Screen for Small Molecule Inhibitors of Nuclear Protein Import
    Larry Gerace; Fiscal Year: 2007
    ..nucleoporins to translocate protein and RNA cargoes across the nuclear envelope, and are regulated by the small GTPase Ran. Up to now, only one class of small molecule inhibitors of the nuclear transport machinery has been described, ..
  14. INHIBITION OF NUCLEAR TRANSPORT BY TUMOR SUPPRESSOR CC3
    Emma Shtivelman; Fiscal Year: 2009
    ..CCS interacts with karyopherins of importin D family, NTF2 (Ran transport receptor) and nucleoporins...
  15. Meiotic spindle formation in Drosophila females
    Kim S McKim; Fiscal Year: 2010
    ..ii) characterize the interactions between Subito and the Passenger proteins or the Ran pathway. This will determine which proteins interact with Subito and what is their function in spindle assembly...
  16. Ian G Macara; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): RCC1 is a guanine nucleotide exchange factor for the Ran GTPase...
  17. CARDIOVIRAL PROTEASES AND COMPARATIVE GENOME STRUCTURE
    Ann C Palmenberg; Fiscal Year: 2013
    ..and molecular pathways of EMCV L (Leader), the first viral (or cellular) protein known to bind and inactivate Ran GTPase cycling, the crucial, ubiquitous regulatory system for all protein and nucleic acid trafficking into and out ..
  18. Method Development for a Next Generation Miniaturized Genomic Screening Platform
    Peter Kahn; Fiscal Year: 2012
    ..tested in Saez's lab at the SCRIPPS Institute in La Jolla California and Pedro Aza's lab at Burnham, who ran a similar screen using conventional 384-well plates...
  19. Nuclear Import Pathways for HPV Macromolecules
    JUNONA F MOROIANU; Fiscal Year: 2010
    ..In addition, we discovered that high risk HPV16 E7 oncoprotein enters the nucleus via a novel Ran-dependent import mechanism...
  20. The Role of CyclophilinA in the Evolution of Retroviral Restriction
    RAYMOND MALFAVON BORJA; Fiscal Year: 2010
    ..in genomics and molecular evolution, I will be attending classes in virology and career development classes (ran by the student-post-doc association at the Fred Hutchinson Cancer Research Center) focused on public speaking, ..
  21. Richard A Cerione; Fiscal Year: 2016
    ..the second pathway links HRG-signaling through the small GTPase Rac to mTOR/p70 S6 kinase and the nuclear GTPase Ran, resulting in the processing of capped mRNAs...
  22. Genetic and Molecular Dissection of RanBP2-Mediated RanGTPase Functions
    Paulo A Ferreira; Fiscal Year: 2013
    ..We will focus on determining the functional relationships between RAN GTPase and one of its high-affinity and multi-binding targets, the RAN-binding protein 2 (RANBP2)...
  23. Effect of RanbpM on amyloid pathology in a mouse model of Alzheimer's disease
    MADEPALLI KRISHNAPPA LAKSHMANA; Fiscal Year: 2009
    ..of A2 production, we used this domain as bait in a yeast 2-hybrid screen, resulting in the identification of Ran-binding protein M (RanbpM), implicated in intracellular membrane trafficking and signaling...
  24. Genetic and Molecular Analyses of Protein Biogenesis in the Neuroretina
    Paulo A Ferreira; Fiscal Year: 2013
    ..b>Ran-binding protein 2 (RanBP2) via two distinct domains, the cyclophilin-like (CLD) and cyclophilin (CY) domains, is a ..
  25. Michael S Gilmore; Fiscal Year: 2016
    ..elegans and murine models respectively. This one line of investigation ran through all groups, and the 2nd generation WTA inhibitor is now advancing through the NIH preclinical development ..
  26. Structural requirements for the nuclear export of HIV RNA
    DAVID SCOTT BOOTH; Fiscal Year: 2013
    ..RNA structure called the Rev Response Element (RRE) and by recruiting the host nuclear export adaptors Crm1 and Ran. To export the RRE, the ternary complex formed between these components must favorably interact with the nuclear ..
  27. John Blenis; Fiscal Year: 2016
    ..previously unknown roles of C3G and various GEFs in mTORC1 activation (aim #1);define how and why AAs regulate the Ran gradient and the nuclear entry of Rags and/or mTORC1 (aim #2);and identify new molecular details of how mTORC1 ..
  28. BRYCE PASCHAL; Fiscal Year: 2016
    ..the levels of reactive oxygen species, which are elevated in HGPS cells, restores nuclear localization of the RanGTPase and Ubc9...
  29. Michael Emmerson Ward; Fiscal Year: 2015
    ..study revealed that PGRN-KO RGCs with nuclear depletion of TDP43 also had loss of expression of the small GTPase Ran, a master regulator of nuclear transport...
  30. PATHWAYS OF NUCLEOCYTOPLASMIC TRANSPORT
    BRYCE PASCHAL; Fiscal Year: 2009
    ..the localization of AR, stress signaling exerts global effects on the nuclear transport machinery by disrupting the Ran gradient which is essential for most nuclear transport pathways...
  31. PAUL STEPHEN MARINEC; Fiscal Year: 2014
    ..Moreover, as part of a larger collaborative effort with the Recombinant Antibody Network (RAN), these studies will help establish a robust and efficient pipeline for the generation of a renewable, validated, ..
  32. Brain Reward-Pathway Function and Neurocomputational Modeling in Anorexia Nervosa
    GUIDO KW FRANK; Fiscal Year: 2012
    ..resonance brain imaging (fMRI) research in Eating Disorders, specifically Restricting Type Anorexia Nervosa (RAN), 2...
  33. RNA Transport Within the Nucleus
    Thoru Pederson; Fiscal Year: 2005
    ..Another aspect of the project will address the intranuclear traffic of these RNAs in relation to proteins known to be involved in nuclear import or RNA export, i.e. the Ran-GTPase and TAP.
  34. CELL ENTRY AND SPREAD BY POLYOMA VIRUS
    THOMAS LIVINGSTON BENJAMIN; Fiscal Year: 2010
    ..investigation in this regard are the oxidoreductase ERp29, protein disulfide isomerase, the derlin chaperones, and Ran GTPase...
  35. Song Tan; Fiscal Year: 2016
    ..These fundamental processes are controlled by localizing the small Ran guanosine triphosphatase (GTPase) protein in its GDP or GTP bound state within the cytoplasm or the nucleus ..
  36. Multivalent Vaccine for Viral Hemorrhagic Fever
    THOMAS WILLIAM GEISBERT; Fiscal Year: 2010
    ..mom ;If oar to- y0 <<m ran {13 be si.ifficient to generate crossSudan GPs...
  37. Anthony A Kossiakoff; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): The RAN is a collaborative international tri-center partnership having the unified goal of generating customized affinity capture reagents to all the human transcription factors...
  38. Bioswellable Absorbable Monofilament Sutures
    Shalaby W Shalaby; Fiscal Year: 2010
    ..polyether-esters for designing macromolecular chains comprising polyethylene glycol or (polyethylene glycol-ran-propylene glycol) end-grafted with cyclic monomers to produce two major types of fiber-forming copolymers having ..
  39. Training In Functional Lipidomics In Cardiovascular &Respiratory Diseases
    Sarah Spiegel; Fiscal Year: 2013
    ..who serve as outstanding role models, and overall by a program steering committee, which has two members who ran highly successful longstanding MD/PhD programs...
  40. Rev-dependent Nuclear Export of RNA
    Mirko Hennig; Fiscal Year: 2010
    ..Rev structure, Rev oligomerization, oligomeric Rev-RRE assembly and the interaction with cellular CRM1 and Ran-GTP? We propose to answer this question by pursuing the following Specific Aims: 1) Understand the function and ..
  41. Physician-Scientist Mentoring at the American Society for Clinical Investigation
    Jonathan A Epstein; Fiscal Year: 2010
    ..Last year, the Joint Meeting ran a significant deficit...
  42. Ryan B Sartor; Fiscal Year: 2016
    ..Susan Henning, PhD as co-director after she moved to UNC in 2007 from Baylor College of Medicine, where she ran a highly effective Pediatric GI training program for 18 years...
  43. Philip J S Stork; Fiscal Year: 2014
    ..We propose that the RA domains of Epac1 and Epac2 link cAMP signaling to the small G proteins, Ran and Ras, respectively...
  44. INTERNATIONAL RESEARCH ETHICS: SHORT COURSE
    Nancy Kass; Fiscal Year: 2000
    ..Twenty-two students/professionals were enrolled, from seven different countries. The course ran from 1:30-5:00 p.m. Monday through Friday for one week...
  45. FACTORS REGULATING TRANSPOSITION IN C. ELEGANS
    John Collins; Fiscal Year: 1992
    ..approaches will be combined to investigate important features of four C elegans transposon families: Tc1, Tc3, Tc4, and Tc5...
  46. CARDIOVIRAL PROTEASES AND COMPARATIVE GENOME STRUCTURE
    Ann Palmenberg; Fiscal Year: 2007
    ..and molecular pathways of EMCV L (Leader), the first viral (or cellular) protein known to bind and inactivate Ran GTPase cycling, the crucial, ubiquitous regulatory system for all protein and nucleic acid trafficking into and out ..
  47. Mechanism of TGF-beta Signaling Termination
    Xin Hua Feng; Fiscal Year: 2010
    ..the dephosphorylated Smad2/3 by PPM1A is ready to be exported out of the nucleus through a pathway dependent of Ran-binding protein RanBP3...
  48. Structure-function analysis of RanBP2 in the neuroretina
    Paulo Ferreira; Fiscal Year: 2006
    ..The focus of this proposal is to understand the role of an unique and large scaffold vertebrate protein, Ran-binding protein 2 (RanBP2/Nup358), in mediating and integrating key steps of intracellular trafficking processes ..
  49. Moleclar Pathogenesis of Retinitis Pigmentosa Type 3
    Paulo Ferreira; Fiscal Year: 2004
    ..pigmentosa GTPase regulator (RPGR) gene in light of its homology to RCC1, a nucleotide-exchange factor for RanGTPase. All RP3-missense mutations to date identified are located in the RCC1-homologous domain...
  50. VIBRATIONAL DYNAMICS IN BIOLOGICAL (HEME) SYSTEMS
    MIRIAM SIMPSON; Fiscal Year: 2002
    ..The proposed research will explore the idea that the events in the first few picoseconds ran be crucial in determining product distributions...
  51. Obscurin Signaling Through its Rho-GEF and PH Domains
    AMBER BOWMAN; Fiscal Year: 2007
    ..I have discovered that a Ran binding protein, RanBPM is a binding partner of the Rho-GEF domain of obscurin...
  52. Relating Decoding and Fluency Development in RD Children
    DONALD COMPTON; Fiscal Year: 2005
    ..The second possible explanation is that other factors such as rapid automatized naming (RAN) speed control fluency development in children with RD...
  53. PEDIATRIC ONCOLOGY GROUP
    Holcombe Grier; Fiscal Year: 2002
    ..Finally Dr. Lipshultz ran at DFCI/CH the pilot studies of late cardiac toxicity from anthracyclines that provides the background data for the ..
  54. RETINOIC ACID--ROLE IN DIFFERENTIATION
    Lorraine Gudas; Fiscal Year: 2002
    ..The P. I. has also identified severa putative Hoxa1 protein target genes, including Ran and "clone 62," a novel gen induced in Hoxa1 overexpressing cells. Over the next grant period the P. I...
  55. FASEB Summer Research Conference on Small G-Proteins
    Linda Van Aelst; Fiscal Year: 2002
    ..be on the function, regulation and structure of five main branches of Ras-related GTPases: Ras, Rho, Rab, Arf and Ran. In particular, special emphasis will be given to the function of these GTPases in physiological processes and ..
  56. (18)O Kinetic isotope effects in G protein GTPases
    STEPHEN SPRANG; Fiscal Year: 2006
    ..GAPs for the small G proteins Ras and Ran, and for the heterotrimeric Galpha proteins, appear to accelerate the GTPase activity of their G protein substrates ..
  57. PSYCHONEUROIMMUNOLOGY AND HIV INFECTION
    Margaret Kemeny; Fiscal Year: 1993
    ..with immune processes prognostic for the development of AIDS, as well as a more rapid development of AIDS in HIV+ ran who participate in the Los Angeles Multi-Center AIDS Cohort Study (MACS)...
  58. Genomic Organization by the Nuclear Export Machinery
    Joe Salas Marco; Fiscal Year: 2006
    ..The proposal will focus primarily on the nucleoporin Nup98 and the Ran GTPase exchange factor Red, since both proteins have been shown to associate with the genome...
  59. NUCLEAR TRANSPORT IN SILICO-A VIRTUAL CELL APPROACH
    Ian Macara; Fiscal Year: 2005
    ..Assembly of carrier/cargo complexes for import are dissociated in the nucleus by the small GTPase, Ran. Assembly of export complexes requires Ran-GTP, and dissociation in the cytoplasm is triggered by Ran-GTP ..
  60. FOAMY VIRUS INFECTION IN VITRO AND IN VIVO
    Maxine Linial; Fiscal Year: 2005
    ..In vitro, HFV is highly cytopathic in some cell types, yet persistent infection ran be obtained in other cells. Little is known about the difference in host cell response...
  61. AR Coactivators, Mechanisms & Roles in Prostate Cancer
    Sophia Tsai; Fiscal Year: 2006
    ..application, we propose to investigate the molecular mechanisms by which SRC family coactivators, p300, and Ran/ARA24 modulate AR transcriptional activity in the context of chromatin by using Xenopus oocytes as a model system...
  62. MEI3 PSEUDOSUBSTRATE LIKE INHIBITOR
    Maureen McLeod; Fiscal Year: 2000
    ..The carboxy-terminal half of the molecule is sufficient to cause meiotic catastrophe and to inhibit Ran-1 in vitro...
  63. PROSPECTIVE STUDY OF HEALTH IN RUNNERS AND WALKERS
    Paul Williams; Fiscal Year: 2002
    ..1%). Additionally, a detectable reduction in breast cancer risk as small as 1.5% per mile run in women is calculated, which is below the 1.7% reduction in risk estimated from other published data. ..
  64. Long-Term Exercise Maintenance via Internet Support
    Paul Williams; Fiscal Year: 2006
    ..1+_10.1 years. Our cohort of 120,000 runners ran >12 mi/wk for an average of 12.4+_7.6 years...
  65. Gene-Specific Responses to Exercise in Discordant Twins
    Paul Williams; Fiscal Year: 2007
    ..DNA samples will be shared with other institutions to cross-validate gene associations identified from training studies or other designs. ..