RAD52

Summary

Gene Symbol: RAD52
Description: RAD52 homolog, DNA repair protein
Alias: DNA repair protein RAD52 homolog, recombination protein RAD52, rhabdomyosarcoma antigen MU-RMS-40.23
Species: human
Products:     RAD52

Top Publications

  1. Shen Z, Denison K, Lobb R, Gatewood J, Chen D. The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues. Genomics. 1995;25:199-206 pubmed
    The yeast Saccharomyces cerevisiae RAD52 gene is involved in DNA double-strand break repair and mitotic/meiotic recombination. The N-terminal amino acid sequence of yeast S...
  2. Chen G, Yuan S, Liu W, Xu Y, Trujillo K, Song B, et al. Radiation-induced assembly of Rad51 and Rad52 recombination complex requires ATM and c-Abl. J Biol Chem. 1999;274:12748-52 pubmed
    ..assays using purified components, phosphorylation of Rad51 by c-Abl enhances complex formation between Rad51 and Rad52, which cooperates with Rad51 in recombination and repair...
  3. Kitao H, Yuan Z. Regulation of ionizing radiation-induced Rad52 nuclear foci formation by c-Abl-mediated phosphorylation. J Biol Chem. 2002;277:48944-8 pubmed
    The RAD52 epistasis group of proteins, including Rad51, Rad52, and Rad54, plays an important role in the homologous recombination repair of double strand breaks...
  4. Grimme J, Honda M, Wright R, Okuno Y, Rothenberg E, Mazin A, et al. Human Rad52 binds and wraps single-stranded DNA and mediates annealing via two hRad52-ssDNA complexes. Nucleic Acids Res. 2010;38:2917-30 pubmed publisher
    b>Rad52 promotes the annealing of complementary strands of DNA bound by replication protein A (RPA) during discrete repair pathways...
  5. Deng X, Prakash A, Dhar K, Baia G, Kolar C, Oakley G, et al. Human replication protein A-Rad52-single-stranded DNA complex: stoichiometry and evidence for strand transfer regulation by phosphorylation. Biochemistry. 2009;48:6633-43 pubmed publisher
    ..b>Rad52 and RPA participate in the repair of double-stranded DNA breaks (DSBs)...
  6. Navadgi V, Shukla A, Rao B. Effect of DNA sequence and nucleotide cofactors on hRad51 binding to ssDNA: role of hRad52 in recruitment. Biochem Biophys Res Commun. 2005;334:696-701 pubmed
    ..These results help us to model important mechanistic steps of hRad51 presynapsis on ssDNA templates. ..
  7. Wu Y, Siino J, Sugiyama T, Kowalczykowski S. The DNA binding preference of RAD52 and RAD59 proteins: implications for RAD52 and RAD59 protein function in homologous recombination. J Biol Chem. 2006;281:40001-9 pubmed
    We examined the double-stranded DNA (dsDNA) binding preference of the Saccharomyces cerevisiae Rad52 protein and its homologue, the Rad59 protein...
  8. Feng Z, Scott S, Bussen W, Sharma G, Guo G, Pandita T, et al. Rad52 inactivation is synthetically lethal with BRCA2 deficiency. Proc Natl Acad Sci U S A. 2011;108:686-91 pubmed publisher
    Synthetic lethality is a powerful approach to study selective cell killing based on genotype. We show that loss of Rad52 function is synthetically lethal with breast cancer 2, early onset (BRCA2) deficiency, whereas there was no impact ..
  9. Kagawa W, Kurumizaka H, Ishitani R, Fukai S, Nureki O, Shibata T, et al. Crystal structure of the homologous-pairing domain from the human Rad52 recombinase in the undecameric form. Mol Cell. 2002;10:359-71 pubmed
    The human Rad52 protein forms a heptameric ring that catalyzes homologous pairing...

More Information

Publications82

  1. Jackson D, Dhar K, Wahl J, Wold M, Borgstahl G. Analysis of the human replication protein A:Rad52 complex: evidence for crosstalk between RPA32, RPA70, Rad52 and DNA. J Mol Biol. 2002;321:133-48 pubmed
    ..b>Rad52 and RPA, along with other members of the Rad52 epistasis group of genes, repair double-stranded DNA breaks (DSBs)...
  2. Beesley J, Jordan S, Spurdle A, Song H, Ramus S, Kjaer S, et al. Association between single-nucleotide polymorphisms in hormone metabolism and DNA repair genes and epithelial ovarian cancer: results from two Australian studies and an additional validation set. Cancer Epidemiol Biomarkers Prev. 2007;16:2557-65 pubmed
    ..in steroid hormone synthesis (SRD5A2, CYP19A1, HSB17B1, and HSD17B4) and DNA repair (XRCC2, XRCC3, BRCA2, and RAD52) using two Australian ovarian cancer case-control studies, comprising a total of 1,466 cases and 1,821 controls of ..
  3. Kim P, Allen C, Wagener B, Shen Z, Nickoloff J. Overexpression of human RAD51 and RAD52 reduces double-strand break-induced homologous recombination in mammalian cells. Nucleic Acids Res. 2001;29:4352-60 pubmed
    ..RAD51 functions with RAD52 and other proteins to effect strand exchange during HR, forming heteroduplex DNA (hDNA) that is resolved by ..
  4. Danoy P, Michiels S, Dessen P, Pignat C, Boulet T, Monet M, et al. Variants in DNA double-strand break repair and DNA damage-response genes and susceptibility to lung and head and neck cancers. Int J Cancer. 2008;123:457-463 pubmed publisher
    ..Haplotype-analysis suggested potential associations (p < 0.05) between lung cancer and 2 genes (RECQL4 and RAD52), which came with q-value of 8%, and between H-N cancer and 1 gene (DNA-PK) but with q-value of 56%...
  5. Ranatunga W, Jackson D, Flowers II R, Borgstahl G. Human RAD52 protein has extreme thermal stability. Biochemistry. 2001;40:8557-62 pubmed
    The human RAD52 protein plays an important role in the earliest stages of chromosomal double-strand break repair via the homologous recombination pathway. Individual subunits of RAD52 associate into seven-membered rings...
  6. Parsons C, Baumann P, Van Dyck E, West S. Precise binding of single-stranded DNA termini by human RAD52 protein. EMBO J. 2000;19:4175-81 pubmed
    The human RAD52 protein, which exhibits a heptameric ring structure, has been shown to bind resected double strand breaks (DSBs), consistent with an early role in meiotic recombination and DSB repair...
  7. Park M, Ludwig D, Stigger E, Lee S. Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells. J Biol Chem. 1996;271:18996-9000 pubmed
    The yeast RAD52 protein is essential for DNA double-strand break repair, and meiotic and mitotic recombination...
  8. Lok B, Carley A, Tchang B, Powell S. RAD52 inactivation is synthetically lethal with deficiencies in BRCA1 and PALB2 in addition to BRCA2 through RAD51-mediated homologous recombination. Oncogene. 2013;32:3552-8 pubmed publisher
    ..Previous work in our laboratory has shown that loss of RAD52 is synthetically lethal with BRCA2 deficiency, while exhibiting no impact on cell growth and viability in BRCA2-..
  9. Mer G, Bochkarev A, Gupta R, Bochkareva E, Frappier L, Ingles C, et al. Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA. Cell. 2000;103:449-56 pubmed
    ..a specific surface that interacts in a similar manner with the DNA repair enzyme UNG2 and repair factors XPA and RAD52, each of which functions in a different repair pathway...
  10. Lloyd J, McGrew D, Knight K. Identification of residues important for DNA binding in the full-length human Rad52 protein. J Mol Biol. 2005;345:239-49 pubmed
    Human Rad52 (HsRad52) is a DNA-binding protein (418 residues) that promotes the catalysis of DNA double strand break repair by the Rad51 recombinase...
  11. Kumar J, Gupta R. Strand exchange activity of human recombination protein Rad52. Proc Natl Acad Sci U S A. 2004;101:9562-7 pubmed
    ..In eukaryotes, double-strand-break repair by homologous recombination requires the Rad52 group of proteins...
  12. Navadgi V, Shukla A, Vempati R, Rao B. DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52. FEBS J. 2006;273:199-207 pubmed
    Purified human Rad51 and Rad52 proteins exhibit multiple oligomeric states, in vitro. Single-stranded DNA (ssDNA) renders high molecular weight aggregates of both proteins into smaller and soluble forms that include even the monomers...
  13. Siraj A, al Rasheed M, Ibrahim M, Siddiqui K, Al Dayel F, Al Sanea O, et al. RAD52 polymorphisms contribute to the development of papillary thyroid cancer susceptibility in Middle Eastern population. J Endocrinol Invest. 2008;31:893-9 pubmed
    ..homologous recombination pathway genes XRCC3 ARG94HIS and THR241MET, RAD51 UTR 15452658T>C, 15455419A>G, RAD52 2259 and GLN221GLU, conserved DNA damage response gene Tp53 PRO47SER, PRO72ARG, Tp53 UTR 7178189A>C and base ..
  14. Sleeth K, Sørensen C, Issaeva N, Dziegielewski J, Bartek J, Helleday T. RPA mediates recombination repair during replication stress and is displaced from DNA by checkpoint signalling in human cells. J Mol Biol. 2007;373:38-47 pubmed
    ..We find that RPA is dispensable for checkpoint kinase 1 (Chk1) activation and that RPA directly binds RAD52 upon replication stress, suggesting a direct role in recombination repair...
  15. Singleton M, Wentzell L, Liu Y, West S, Wigley D. Structure of the single-strand annealing domain of human RAD52 protein. Proc Natl Acad Sci U S A. 2002;99:13492-7 pubmed
    In eukaryotic cells, RAD52 protein plays a central role in genetic recombination and DNA repair by (i) promoting the annealing of complementary single-stranded DNA and (ii) stimulation of the RAD51 recombinase...
  16. Rothenberg E, Grimme J, Spies M, Ha T. Human Rad52-mediated homology search and annealing occurs by continuous interactions between overlapping nucleoprotein complexes. Proc Natl Acad Sci U S A. 2008;105:20274-9 pubmed publisher
    The Rad52 protein has critical functions in distinct pathways of the homology-directed DNA repair, one of which is to promote the annealing of complementary strands of DNA...
  17. Shin A, Lee K, Ahn B, Park C, Noh S, Park D, et al. Genotype-phenotype relationship between DNA repair gene genetic polymorphisms and DNA repair capacity. Asian Pac J Cancer Prev. 2008;9:501-5 pubmed
    ..repair genes included were those involved in double-strand break repair (ATM, XRCC2, XRCC4, XRCC6, LIG4, RAD51, RAD52), base excision repair (LIG1), nucleotide excision repair (ERCC1), and mismatch repair (hMLH1)...
  18. Baumann P, West S. Heteroduplex formation by human Rad51 protein: effects of DNA end-structure, hRP-A and hRad52. J Mol Biol. 1999;291:363-74 pubmed
    ..Joint molecule formation by hRad51 was stimulated or inhibited by hRad52, dependent upon the reaction conditions. The inhibitory effect could be overcome by the presence of hRP-A or excess heterologous DNA. ..
  19. McIlwraith M, West S. DNA repair synthesis facilitates RAD52-mediated second-end capture during DSB repair. Mol Cell. 2008;29:510-6 pubmed publisher
    ..strand of a D loop, leads to capture and annealing of the second end of a resected DSB in reactions mediated by RAD52 protein...
  20. Kagawa W, Kagawa A, Saito K, Ikawa S, Shibata T, Kurumizaka H, et al. Identification of a second DNA binding site in the human Rad52 protein. J Biol Chem. 2008;283:24264-73 pubmed publisher
    b>Rad52 plays essential roles in homology-dependent double-strand break repair. Various studies have established the functions of Rad52 in Rad51-dependent and Rad51-independent repair processes...
  21. Timofeeva M, Hung R, Rafnar T, Christiani D, Field J, Bickeböller H, et al. Influence of common genetic variation on lung cancer risk: meta-analysis of 14 900 cases and 29 485 controls. Hum Mol Genet. 2012;21:4980-95 pubmed publisher
    ..Several other genetic regions including variants of CHEK2 (22q12), TP53BP1 (15q15) and RAD52 (12p13) have been demonstrated to influence lung cancer risk in candidate- or pathway-based analyses...
  22. Navadgi V, Dutta A, Rao B. Human Rad52 facilitates a three-stranded pairing that follows no strand exchange: a novel pairing function of the protein. Biochemistry. 2003;42:15237-51 pubmed
    Human Rad52 protein, by analogy with the genetics of yeast Rad52, is believed to mediate a pathway of homologous recombination even independent of Rad51...
  23. Cramer Morales K, Nieborowska Skorska M, SCHEIBNER K, Padget M, Irvine D, Sliwinski T, et al. Personalized synthetic lethality induced by targeting RAD52 in leukemias identified by gene mutation and expression profile. Blood. 2013;122:1293-304 pubmed publisher
    ..HRR usually depends on BRCA1/2-RAD51, and RAD52-RAD51 serves as back-up...
  24. Sugiyama T, Kowalczykowski S. Rad52 protein associates with replication protein A (RPA)-single-stranded DNA to accelerate Rad51-mediated displacement of RPA and presynaptic complex formation. J Biol Chem. 2002;277:31663-72 pubmed
    ..contrast, RPA inhibits Rad51 nucleoprotein complex formation by prior binding to single-stranded DNA (ssDNA), but Rad52 protein alleviates this inhibition...
  25. Yáñez R, Porter A. Differential effects of Rad52p overexpression on gene targeting and extrachromosomal homologous recombination in a human cell line. Nucleic Acids Res. 2002;30:740-8 pubmed
    Overexpression of the RAD52 epistasis group of gene products is a convenient way to investigate their in vivo roles in homologous recombination (HR) and DNA repair...
  26. Van Dyck E, Stasiak A, Stasiak A, West S. Visualization of recombination intermediates produced by RAD52-mediated single-strand annealing. EMBO Rep. 2001;2:905-9 pubmed
    ..In yeast, DSB repair requires RAD52, a protein that plays a critical role in homologous recombination...
  27. Stasiak A, Larquet E, Stasiak A, Muller S, Engel A, Van Dyck E, et al. The human Rad52 protein exists as a heptameric ring. Curr Biol. 2000;10:337-40 pubmed
    The RAD52 epistasis group was identified in yeast as a group of genes required to repair DNA damaged by ionizing radiation [1]...
  28. Muris D, Bezzubova O, Buerstedde J, Vreeken K, Balajee A, Osgood C, et al. Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination. Mutat Res. 1994;315:295-305 pubmed
    The RAD52 gene of Saccharomyces cerevisiae is required for recombinational repair of double-strand breaks...
  29. Cao J, Luo C, Peng R, Guo Q, Wang K, Wang P, et al. MiRNA-binding site functional polymorphisms in DNA repair genes RAD51, RAD52, and XRCC2 and breast cancer risk in Chinese population. Tumour Biol. 2016;: pubmed
    RAD51, RAD52, and XRCC2 are all involved in DNA homologous recombinational repair, and there are interactions among those genes. Polymorphisms in 3'-UTR of DNA repair genes may change DNA repair capacity by regulating gene expression...
  30. Wei L, Nakajima S, Böhm S, Bernstein K, Shen Z, Tsang M, et al. DNA damage during the G0/G1 phase triggers RNA-templated, Cockayne syndrome B-dependent homologous recombination. Proc Natl Acad Sci U S A. 2015;112:E3495-504 pubmed publisher
    ..However, assembly of RPA1, RAD51C, RAD51, and RAD52 at such sites is strictly governed by active transcription and requires both wild-type Cockayne syndrome protein B ..
  31. Baynton K, Otterlei M, Bjørås M, Von Kobbe C, Bohr V, Seeberg E. WRN interacts physically and functionally with the recombination mediator protein RAD52. J Biol Chem. 2003;278:36476-86 pubmed
    ..report a novel physical and functional interaction between WRN and the homologous recombination mediator protein RAD52. Fluorescence resonance energy transfer (FRET) analyses show that WRN and RAD52 form a complex in vivo that co-..
  32. Ma C, Kwon Y, Sung P, Greene E. Human RAD52 interactions with replication protein A and the RAD51 presynaptic complex. J Biol Chem. 2017;292:11702-11713 pubmed publisher
    b>Rad52 is a highly conserved protein involved in the repair of DNA damage. Human RAD52 has been shown to mediate single-stranded DNA (ssDNA) and is synthetic lethal with mutations in other key recombination proteins...
  33. Westmoreland J, Mihalevic M, Bernstein K, Resnick M. The global role for Cdc13 and Yku70 in preventing telomere resection across the genome. DNA Repair (Amst). 2018;62:8-17 pubmed publisher
    ..The previously reported Rad52 nuclear centers of repair for random DSBs also appear in cells with many resected telomere ends, suggesting a ..
  34. Raykov V, Marvin M, Louis E, Maringele L. Telomere Dysfunction Triggers Palindrome Formation Independently of Double-Strand Break Repair Mechanisms. Genetics. 2016;203:1659-68 pubmed publisher
    ..Moreover RAD51, important for certain HR-derived mechanisms, had little effect. Furthermore RAD52, which is essential for HR in yeast, appeared to decrease the number of palindromes in cells proliferating without ..
  35. Lafuente Barquero J, Luke Glaser S, Graf M, Silva S, Gómez González B, Lockhart A, et al. The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage. PLoS Genet. 2017;13:e1007136 pubmed publisher
    ..crucial to prevent the accumulation of RNA-DNA hybrids and RNA-DNA hybrid-dependent DNA damage, as determined by Rad52 foci. Mph1 forms foci when RNA-DNA hybrids accumulate, e.g...
  36. Demin A, Lee M, Lee C, Seo Y. GSK-3? Homolog Rim11 and the Histone Deacetylase Complex Ume6-Sin3-Rpd3 Are Involved in Replication Stress Response Caused by Defects in Dna2. Genetics. 2017;206:829-842 pubmed publisher
    ..Moreover, multicopy suppression by Rim11/Ume6 requires the presence of sister-chromatid recombination mediated by Rad52/Rad59 proteins, but not vice versa...
  37. Ouenzar F, Lalonde M, Laprade H, Morin G, Gallardo F, Tremblay Belzile S, et al. Cell cycle-dependent spatial segregation of telomerase from sites of DNA damage. J Cell Biol. 2017;216:2355-2371 pubmed publisher
    ..RNA remains nucleolar in most G2/M cells but accumulates in the nucleoplasm and colocalizes with DSBs in rad52Δ cells, leading to de novo telomere additions...
  38. Huang S, Williams J, Arana M, Kunkel T, Pommier Y. Topoisomerase I-mediated cleavage at unrepaired ribonucleotides generates DNA double-strand breaks. EMBO J. 2017;36:361-373 pubmed publisher
    ..We show that RNase H2-deficient yeast cells displayed elevated frequency of Rad52 foci, inactivation of RNase H2 and RAD52 led to synthetic lethality, and combined loss of RNase H2 and RAD51 ..
  39. Otterlei M, Bruheim P, Ahn B, Bussen W, Karmakar P, Baynton K, et al. Werner syndrome protein participates in a complex with RAD51, RAD54, RAD54B and ATR in response to ICL-induced replication arrest. J Cell Sci. 2006;119:5137-46 pubmed
    ..relocated from nucleoli to arrested replication forks in the nucleoplasm where it interacted with the HR protein RAD52. In this study, we use fluorescence resonance energy transfer (FRET) and immune-precipitation experiments to ..
  40. Miyoshi A, Kito K, Aramoto T, Abe Y, Kobayashi N, Ueda N. Identification of CGI-121, a novel PRPK (p53-related protein kinase)-binding protein. Biochem Biophys Res Commun. 2003;303:399-405 pubmed
    ..In addition, coprecipitation of p53 using recombinant PRPK was inhibited by adding recombinant CGI-121 in vitro, suggesting that CGI-121 could act as a potent inhibitor of the binding of PRPK to p53. ..
  41. Morrill S, Exner A, Babokhov M, Reinfeld B, Fuchs S. DNA Instability Maintains the Repeat Length of the Yeast RNA Polymerase II C-terminal Domain. J Biol Chem. 2016;291:11540-50 pubmed publisher
    ..We also determined that RAD52 is necessary for CTD repeat expansion but not contraction, identifying a role for recombination in repeat ..
  42. Bi B, Rybalchenko N, Golub E, Radding C. Human and yeast Rad52 proteins promote DNA strand exchange. Proc Natl Acad Sci U S A. 2004;101:9568-72 pubmed
    Studies of rad52 mutants in Saccharomyces cerevisiae have revealed a critical role of Rad52 protein in double-strand break repair and meiosis, and roles in both RAD51-dependent and -independent pathways of recombination...
  43. Gualberto J, Le Ret M, Beator B, Kühn K. The RAD52-like protein ODB1 is required for the efficient excision of two mitochondrial introns spliced via first-step hydrolysis. Nucleic Acids Res. 2015;43:6500-10 pubmed publisher
    ..Some of these belong to protein families initially characterised to perform unrelated functions. The RAD52-like ODB1 protein has been reported to have roles in homologous recombination-dependent DNA repair in the nuclear ..
  44. Paliwal S, Kanagaraj R, Sturzenegger A, Burdova K, Janscak P. Human RECQ5 helicase promotes repair of DNA double-strand breaks by synthesis-dependent strand annealing. Nucleic Acids Res. 2014;42:2380-90 pubmed publisher
    ..non-crossover products during DNA double-strand break-induced HR and counteracts the inhibitory effect of RAD51 on RAD52-mediated DNA annealing in vitro and in vivo...
  45. De Tullio L, Kaniecki K, Kwon Y, Crickard J, Sung P, Greene E. Yeast Srs2 Helicase Promotes Redistribution of Single-Stranded DNA-Bound RPA and Rad52 in Homologous Recombination Regulation. Cell Rep. 2017;21:570-577 pubmed publisher
    ..Remarkably, Srs2 also readily removes the recombination mediator Rad52 from RPA-ssDNA and, in doing so, promotes rapid redistribution of both Rad52 and RPA...
  46. Sacher M, Pfander B, Hoege C, Jentsch S. Control of Rad52 recombination activity by double-strand break-induced SUMO modification. Nat Cell Biol. 2006;8:1284-90 pubmed
    ..Central to this process is Rad52, a conserved homo-oligomeric ring-shaped protein, which mediates the exchange of the early recombination factor ..
  47. Nair A, Agarwal R, Chittela R. Biochemical characterization of plant Rad52 protein from rice (Oryza sativa). Plant Physiol Biochem. 2016;106:108-17 pubmed publisher
    ..Of all the genes promoting HR, Rad52 (Radiation sensitive 52) is an important gene which is found to be highly conserved across different species...
  48. Du Y, Zhou J, Fan J, Shen Z, Chen X. Streamline proteomic approach for characterizing protein-protein interaction network in a RAD52 protein complex. J Proteome Res. 2009;8:2211-7 pubmed publisher
    ..With this streamlined approach, we studied the protein complexes that are associated with a DNA recombination protein RAD52. In the initial phase, multiple proteins both known and unknown to interact with RAD52 were identified ..
  49. Bordeianu G, Petrescu Danila E, Ungureanu D, Stoica B, Stanescu R, Cernomaz A, et al. Rad52 protein function study by fluorescence tagging. Rev Med Chir Soc Med Nat Iasi. 2013;117:444-9 pubmed
    b>Rad52 protein plays a significant role in DNA lesions repair by homologous recombination in eukariotic cells...
  50. Shen Z, Huhn S. PTEN sumo-wrestles human RAD52 to mystery land. Cell Cycle. 2013;12:3585 pubmed publisher
  51. Shukla A, Navadgi V, Mallikarjuna K, Rao B. Interaction of hRad51 and hRad52 with MCM complex: a cross-talk between recombination and replication proteins. Biochem Biophys Res Commun. 2005;329:1240-5 pubmed
    Human Rad51 and Rad52 are implicated in DNA repair during replication...
  52. Shi T, Yang G, Tu X, Yang J, Qian J, Wu X, et al. RAD52 variants predict platinum resistance and prognosis of cervical cancer. PLoS ONE. 2012;7:e50461 pubmed publisher
    b>RAD52 is an important but not well characterized homologous recombination repair gene that can bind to single-stranded DNA ends and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands...
  53. de Souza Pinto N, Maynard S, Hashiguchi K, Hu J, Muftuoglu M, Bohr V. The recombination protein RAD52 cooperates with the excision repair protein OGG1 for the repair of oxidative lesions in mammalian cells. Mol Cell Biol. 2009;29:4441-54 pubmed publisher
    ..We report here that OGG1 interacts with the recombination protein RAD52 in vitro and in vivo...
  54. Saotome M, Saito K, Onodera K, Kurumizaka H, Kagawa W. Structure of the human DNA-repair protein RAD52 containing surface mutations. Acta Crystallogr F Struct Biol Commun. 2016;72:598-603 pubmed publisher
    The Rad52 protein is a eukaryotic single-strand DNA-annealing protein that is involved in the homologous recombinational repair of DNA double-strand breaks...
  55. Honda M, Okuno Y, Yoo J, Ha T, Spies M. Tyrosine phosphorylation enhances RAD52-mediated annealing by modulating its DNA binding. EMBO J. 2011;30:3368-82 pubmed publisher
    b>RAD52 protein has an important role in homology-directed DNA repair by mediating RAD51 nucleoprotein filament formation on single-stranded DNA (ssDNA) protected by replication protein-A (RPA) and annealing of RPA-coated ssDNA...
  56. Hengel S, Spies M, Spies M. Small-Molecule Inhibitors Targeting DNA Repair and DNA Repair Deficiency in Research and Cancer Therapy. Cell Chem Biol. 2017;24:1101-1119 pubmed publisher
    ..inhibitors of the DNA repair proteins, from PARP1, a paradigm case for clinically successful small-molecule inhibitors, to coveted new targets, such as RAD51 recombinase, RAD52 DNA repair protein, MRE11 nuclease, and WRN DNA helicase.
  57. Riches L, Lynch A, Gooderham N. A molecular beacon approach to detecting RAD52 expression in response to DNA damage in human cells. Toxicol In Vitro. 2010;24:652-60 pubmed publisher
    Although DNA damage proteins are infrequently regulated at the transcriptional level, RAD52 mRNA levels appear to be significantly induced in human cells following methyl methanesulphonate (MMS) and Etoposide treatment...
  58. Park M. Expression of human RAD52 confers resistance to ionizing radiation in mammalian cells. J Biol Chem. 1995;270:15467-70 pubmed
    ..Although RAD52 has been shown to be important for DSB repair in yeasts, its role in DSB repair in mammalian cells has not been ..
  59. Welty S, Teng Y, Liang Z, Zhao W, Sanders L, Greenamyre J, et al. RAD52 is required for RNA-templated recombination repair in post-mitotic neurons. J Biol Chem. 2018;293:1353-1362 pubmed publisher
    ..Here we observed that the HR repair protein RAD52 is recruited to sites of DNA DSBs in terminally differentiated, post-mitotic neurons...
  60. Chen C, Huang J, Wang T, Kuo C, Wang C, Wang S, et al. Dihydrocoumarin, an HDAC Inhibitor, Increases DNA Damage Sensitivity by Inhibiting Rad52. Int J Mol Sci. 2017;18: pubmed publisher
    ..Following DNA damage, Rad52 recombinase was revealed to be inhibited by DHC, which results in deficiencies in DSB repair and prolonged DNA ..
  61. Lenart P, Zlamal F, Machal J, Hlinomaz O, Groch L, Bienertova Vasku J. Increased age-adjusted hazard of death associated with a common single nucleotide polymorphism of the human RAD52 gene in a cardiovascular cohort. Mech Ageing Dev. 2017;167:56-63 pubmed publisher
    ..four common polymorphisms (rs2155209, rs7963551, rs17105278, rs2735383) in four selected DSB repair genes (MRE11A, RAD52, RAD51B, NBS1) influence the hazard of age-adjusted death in a cohort of patients with typical symptoms of ..
  62. Couto P, Bastos Rodrigues L, Schayek H, Melo F, Lisboa R, Miranda D, et al. Spectrum of germline mutations in smokers and non-smokers in Brazilian non-small-cell lung cancer (NSCLC) patients. Carcinogenesis. 2017;38:1112-1118 pubmed publisher
    ..and THBS1 genes were identified exclusively in smoker patients, and four germline missense variants in PLCD1, RAD52, CP and CDC6 genes were identified solely in non-smokers. There were 4/45 (8...
  63. Han J, Hankinson S, De Vivo I, Colditz G, Hunter D. No association between a stop codon polymorphism in RAD52 and breast cancer risk. Cancer Epidemiol Biomarkers Prev. 2002;11:1138-9 pubmed
  64. Bhowmick R, Minocherhomji S, Hickson I. RAD52 Facilitates Mitotic DNA Synthesis Following Replication Stress. Mol Cell. 2016;64:1117-1126 pubmed publisher
    ..In contrast, MiDAS is RAD52 dependent, and RAD52 is required for the timely recruitment of MUS81 and POLD3 to CFSs in early mitosis...
  65. Xie Y, Kerscher O, Kroetz M, McConchie H, Sung P, Hochstrasser M. The yeast Hex3.Slx8 heterodimer is a ubiquitin ligase stimulated by substrate sumoylation. J Biol Chem. 2007;282:34176-84 pubmed
    ..Our data reveal a novel mechanism of substrate targeting in which sumoylation of a protein can help trigger its subsequent ubiquitination by recruiting a SUMO-binding ubiquitin ligase. ..
  66. Motycka T, Bessho T, Post S, Sung P, Tomkinson A. Physical and functional interaction between the XPF/ERCC1 endonuclease and hRad52. J Biol Chem. 2004;279:13634-9 pubmed
    ..We propose that the ternary complex of hRad52 and XPF/ERCC1 is the active species that processes recombination intermediates generated during the repair of DNA double strand breaks and in homology-dependent gene targeting events. ..
  67. Lee K, Choi J, Kang C, Kang C, Park S, Cho H, et al. Genetic polymorphisms of selected DNA repair genes, estrogen and progesterone receptor status, and breast cancer risk. Clin Cancer Res. 2005;11:4620-6 pubmed
    ..XRCC2 Arg188His, XRCC4 921G > T, XRCC6 1796G > T, LIG4 1977T/C, RAD51 135G > C, 172G > T, RAD52 2259C > T, LIG1 551A > C, ERCC1 8092A > C, 354C > T, hMLH1 -93G > A, and Ile219Val)...
  68. Wan L, Lou J, Xia Y, Su B, Liu T, Cui J, et al. hPrimpol1/CCDC111 is a human DNA primase-polymerase required for the maintenance of genome integrity. EMBO Rep. 2013;14:1104-12 pubmed publisher
    ..Both RPA1 binding and the primase activity of hPrimpol1 are required for its cellular function during DNA replication. Our results indicate that hPrimpol1 is a novel factor involved in the response to DNA replication stress. ..
  69. Lieberman R, Xiong D, James M, Han Y, Amos C, Wang L, et al. Functional characterization of RAD52 as a lung cancer susceptibility gene in the 12p13.33 locus. Mol Carcinog. 2016;55:953-63 pubmed publisher
    Recent genome-wide association studies have identified variations in the recombination repair gene, RAD52, that are associated with increased lung cancer risk, and particularly with the development of lung squamous cell carcinomas (LUSC)...
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    As an important member in homologous recombination repair, RAD52 plays a crucial part in maintaining genomic stability and prevent carcinogenesis...
  71. Kan Y, Batada N, Hendrickson E. Human somatic cells deficient for RAD52 are impaired for viral integration and compromised for most aspects of homology-directed repair. DNA Repair (Amst). 2017;55:64-75 pubmed publisher
    ..The inactivation of the fungal radiation sensitive 52 (RAD52) gene, which has both recombination mediator and single-strand annealing (SSA) activities in vitro, leads to ..
  72. Shen Z, Pardington Purtymun P, Comeaux J, Moyzis R, Chen D. UBL1, a human ubiquitin-like protein associating with human RAD51/RAD52 proteins. Genomics. 1996;36:271-9 pubmed
    The RAD51/RAD52-dependent DNA repair pathway is involved in DNA recombination and DNA double-strand break repair in yeast...
  73. Naccarati A, Rosa F, Vymetalkova V, Barone E, Jiraskova K, Di Gaetano C, et al. Double-strand break repair and colorectal cancer: gene variants within 3' UTRs and microRNAs binding as modulators of cancer risk and clinical outcome. Oncotarget. 2016;7:23156-69 pubmed publisher
    ..In colon cancer patients, the rs2155209 CC genotype was associated with shorter survival while the TT genotype of RAD52 rs11226 with longer survival when both compared with their respective more frequent genotypes (HR 1.63, 95% CI 1...