RAB5B

Summary

Gene Symbol: RAB5B
Description: RAB5B, member RAS oncogene family
Alias: ras-related protein Rab-5B
Species: human
Products:     RAB5B

Top Publications

  1. Zhang M, Robitaille M, Showalter A, Huang X, Liu Y, Bhattacharjee A, et al. Progesterone receptor membrane component 1 is a functional part of the glucagon-like peptide-1 (GLP-1) receptor complex in pancreatic β cells. Mol Cell Proteomics. 2014;13:3049-62 pubmed publisher
    ..Three novel GLP-1R interactors (PGRMC1, Rab5b, and Rab5c) were further validated through co-immunoprecipitation/immunoblotting, fluorescence resonance energy ..
  2. Zhao X, Yi Y, Meng C, Fang N. MiRNA-575 suppresses angiogenesis by targeting Rab5-MEK-ERK pathway in endothelial cells. Biosci Rep. 2019;39: pubmed publisher
    ..Inhibition of miR-575 performed the reverse effects of HUVECs. We further suggested Rab5B was the downstream target of miR-575 and knockdown of Rab5B significantly inhibited migration and proliferation of ..
  3. Maheshwari D, Yadav R, Rastogi R, Jain A, Tripathi S, Mukhopadhyay A, et al. Structural and Biophysical Characterization of Rab5a from Leishmania Donovani. Biophys J. 2018;: pubmed publisher
    Leishmania donovani possess two isoforms of Rab5 (Rab5a and Rab5b), which are involved in fluid phase and receptor-mediated endocytosis, respectively...
  4. Zhu K, Li S, Liu J, Hong Y, Chen Z, Du Y. Role of RAB5A in FSHR-mediated signal transduction in human granulosa cells. Reproduction. 2018;155:505-514 pubmed publisher
    ..i>RAB5B, which is identified by a genome-wide association study as a risk locus for this syndrome, encodes a small ..
  5. Kong X, Zhang J, Li J, Shao J, Fang L. MiR-130a-3p inhibits migration and invasion by regulating RAB5B in human breast cancer stem cell-like cells. Biochem Biophys Res Commun. 2018;501:486-493 pubmed publisher
    ..We also confirmed that RAB5B is directly down-regulated by miR-130a-3p...
  6. Rached F, Ndjembo Ezougou C, Chandran S, Talabani H, Yera H, Dandavate V, et al. Construction of a Plasmodium falciparum Rab-interactome identifies CK1 and PKA as Rab-effector kinases in malaria parasites. Biol Cell. 2012;104:34-47 pubmed publisher
    ..We demonstrate its potential by wet-bench testing three predictions; that casein kinase-1 (PfCK1) is a specific Rab5B interacting protein and that the catalytic subunit of cAMP-dependent protein kinase A (PfPKA-C) is a PfRab5A and ..
  7. Ranadheera C, Coombs K, Kobasa D. Comprehending a Killer: The Akt/mTOR Signaling Pathways Are Temporally High-Jacked by the Highly Pathogenic 1918 Influenza Virus. EBioMedicine. 2018;32:142-163 pubmed publisher
    ..IAV1918 also strongly induced Rab5b expression whereas all other tested viruses induced minor up-regulation or down-regulation...
  8. Saxena R, Georgopoulos N, Braaten T, Bjonnes A, Koika V, Panidis D, et al. Han Chinese polycystic ovary syndrome risk variants in women of European ancestry: relationship to FSH levels and glucose tolerance. Hum Reprod. 2015;30:1454-9 pubmed publisher
    ..in the intron of FSHR was associated with PCOS and lower FSH levels, while another variant rs705702-G near the RAB5B and SUOX genes was associated with insulin and glucose levels after oral glucose testing in women with PCOS of ..
  9. Segawa T, Hazeki K, Nigorikawa K, Morioka S, Guo Y, Takasuga S, et al. Inpp5e increases the Rab5 association and phosphatidylinositol 3-phosphate accumulation at the phagosome through an interaction with Rab20. Biochem J. 2014;464:365-75 pubmed publisher
    ..The expression of a constitutively active form of Rab5b in the shInpp5e cells rescued the PtdIns3P accumulation...

More Information

Publications52

  1. McAllister J, Legro R, Modi B, Strauss J. Functional genomics of PCOS: from GWAS to molecular mechanisms. Trends Endocrinol Metab. 2015;26:118-24 pubmed publisher
    ..association studies (GWAS) identified several PCOS candidate loci including LHCGR, FSHR, ZNF217, YAP1, INSR, RAB5B, and C9orf3...
  2. Ali N, Jurczyluk J, Shay G, Tnimov Z, Alexandrov K, Munoz M, et al. A highly sensitive prenylation assay reveals in vivo effects of bisphosphonate drug on the Rab prenylome of macrophages outside the skeleton. Small Gtpases. 2015;6:202-11 pubmed publisher
    ..Finally, we also detected a clear effect of subcutaneous ZOL administration in vivo on the prenylation of Rab1A, Rab5B, Rab7A and Rab14 in mouse peritoneal macrophages, confirming that systemic treatment with bisphosphonate drug can ..
  3. Peinado H, Alečković M, Lavotshkin S, Matei I, Costa Silva B, Moreno Bueno G, et al. Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. Nat Med. 2012;18:883-91 pubmed publisher
    ..RAB1A, RAB5B, RAB7 and RAB27A, regulators of membrane trafficking and exosome formation, were highly expressed in melanoma ..
  4. Kokosar M, Benrick A, Perfilyev A, Fornes R, Nilsson E, Maliqueo M, et al. Epigenetic and Transcriptional Alterations in Human Adipose Tissue of Polycystic Ovary Syndrome. Sci Rep. 2016;6:22883 pubmed publisher
    ..05). Six out of twenty selected genes with largest expression difference (CYP1B1, GPT), genes linked to PCOS (RAB5B) or type 2 diabetes (PPARG, SVEP1), and methylation (DMAP1) were replicated in a separate case-control study...
  5. Sun Y, Yuan Y, Yang H, Li J, Feng T, Ouyang Y, et al. Association between Common Genetic Variants and Polycystic Ovary Syndrome Risk in a Chinese Han Population. J Clin Res Pediatr Endocrinol. 2016;8:405-410 pubmed publisher
    ..We found that rs705702 in the RAB5B/SUOX was associated with PCOS (odds ratio=1.42; 95% confidence interval=1.08-1.87, p=0...
  6. Bridgewater R, Streuli C, Caswell P. Extracellular matrix promotes clathrin-dependent endocytosis of prolactin and STAT5 activation in differentiating mammary epithelial cells. Sci Rep. 2017;7:4572 pubmed publisher
    ..Prolactin is internalised into early endosomes, where the master early endosome regulator Rab5b promotes STAT5 phosphorylation...
  7. Atherton P, Lausecker F, Harrison A, Ballestrem C. Low-intensity pulsed ultrasound promotes cell motility through vinculin-controlled Rac1 GTPase activity. J Cell Sci. 2017;130:2277-2291 pubmed publisher
    ..FA) protein vinculin, and both focal adhesion kinase (FAK, also known as PTK2) and Rab5 (both the Rab5a and Rab5b isoforms) have key roles in regulating these effects...
  8. Bersani F, Lingua M, Morena D, Foglizzo V, Miretti S, Lanzetti L, et al. Deep Sequencing Reveals a Novel miR-22 Regulatory Network with Therapeutic Potential in Rhabdomyosarcoma. Cancer Res. 2016;76:6095-6106 pubmed
    ..tumor dissemination in vivo Gene expression profiling analysis of miR-22-expressing cells suggested TACC1 and RAB5B as possible direct miR-22 targets...
  9. Rastogi R, Verma J, Kapoor A, Langsley G, Mukhopadhyay A. Rab5 Isoforms Specifically Regulate Different Modes of Endocytosis in Leishmania. J Biol Chem. 2016;291:14732-46 pubmed publisher
    ..Here, we cloned, expressed, and characterized Rab5a and Rab5b from Leishmania and found that both of them are localized in the early endosome...
  10. Law F, Seo J, Wang Z, Deleon J, Bolis Y, Brown A, et al. The VPS34 PI3K negatively regulates RAB-5 during endosome maturation. J Cell Sci. 2017;130:2007-2017 pubmed publisher
    ..with large late endosomes, as with loss of TBC-2, and that Rab5 activity (mice have two Rab5 isoforms, Rab5a and Rab5b) is increased in Vps34-knockout mouse embryonic fibroblasts (Vps34 is also known as PIK3C3 in mammals)...
  11. Millino C, Maretto I, Pacchioni B, Digito M, De Paoli A, Canzonieri V, et al. Gene and MicroRNA Expression Are Predictive of Tumor Response in Rectal Adenocarcinoma Patients Treated With Preoperative Chemoradiotherapy. J Cell Physiol. 2017;232:426-435 pubmed publisher
    ..The miR-630 appeared only with the NR patients and was anti-correlated with a single transcript: RAB5B. After PAM, the following eight transcripts were strong predictors of tumor response: TMEM188, ITGA2, NRG, TRAM1, ..
  12. Chen Y, Yang W, Li X, Yang H, Xu Z, Yu S. α-Synuclein-induced internalization of NMDA receptors in hippocampal neurons is associated with reduced inward current and Ca(2+) influx upon NMDA stimulation. Neuroscience. 2015;300:297-306 pubmed publisher
    ..Neurons with elevated α-syn also showed a profound increase in Rab5B, an isoform of the small GTPases essential for NMDA receptor endocytosis...
  13. Wilson D, Wilson M. Identification and subcellular localization of human rab5b, a new member of the ras-related superfamily of GTPases. J Clin Invest. 1992;89:996-1005 pubmed
    ..In light of this homology, we have named this new member of the GTPase superfamily "rab5b." Northern analysis using the rab5b cDNA as a probe revealed a 3...
  14. Zou L, Zhou J, Zhang J, Li J, Liu N, Chai L, et al. The GTPase Rab3b/3c-positive recycling vesicles are involved in cross-presentation in dendritic cells. Proc Natl Acad Sci U S A. 2009;106:15801-6 pubmed publisher
    ..Together, these data demonstrate that Rab3b/3c-positive recycling vesicles are involved in and may constitute one of the recycling compartments in exogenous antigen cross-presentation. ..
  15. Pisitkun T, Shen R, Knepper M. Identification and proteomic profiling of exosomes in human urine. Proc Natl Acad Sci U S A. 2004;101:13368-73 pubmed
    ..The results indicate that exosome isolation may provide an efficient first step in biomarker discovery in urine. ..
  16. Shin N, Jeong H, Kwon J, Heo H, Kwon J, Yun H, et al. LRRK2 regulates synaptic vesicle endocytosis. Exp Cell Res. 2008;314:2055-65 pubmed publisher
    ..In this study, using yeast two-hybrid screening, we report the identification of Rab5b as an LRRK2-interacting protein...
  17. Fuchs E, Haas A, Spooner R, Yoshimura S, Lord J, Barr F. Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways. J Cell Biol. 2007;177:1133-43 pubmed
    ..These results suggest that Shiga toxin trafficking to the Golgi is a multistep process controlled by several Rab GAPs and their target Rabs and that this process is discrete from ligand-induced EGF receptor trafficking. ..
  18. Chiariello M, Bruni C, Bucci C. The small GTPases Rab5a, Rab5b and Rab5c are differentially phosphorylated in vitro. FEBS Lett. 1999;453:20-4 pubmed
    ..kinase 1 but not by extracellular-regulated kinase 2, while cdc2 kinase preferentially phosphorylates Ser-123 of Rab5b. These findings strongly suggest that phosphorylation could be important to differentially regulate the function ..
  19. Saito K, Murai J, Kajiho H, Kontani K, Kurosu H, Katada T. A novel binding protein composed of homophilic tetramer exhibits unique properties for the small GTPase Rab5. J Biol Chem. 2002;277:3412-8 pubmed
    ..Possible roles of the quaternary structure have been discussed in terms of the Rab5-mediated signaling. ..
  20. Van Den Berg J, Aten J, Annink C, Ravesloot J, Weber E, Weening J. Interleukin-4 and -13 promote basolateral secretion of H(+) and cathepsin L by glomerular epithelial cells. Am J Physiol Renal Physiol. 2002;282:F26-33 pubmed
    ..The effects of IL-4 and IL-13 were accompanied by redistribution of the small GTPases Rab5b and Rab7, as shown by confocal immunofluorescence studies...
  21. Gisselberg J, Zhang L, Elias J, Yeh E. The Prenylated Proteome of Plasmodium falciparum Reveals Pathogen-specific Prenylation Activity and Drug Mechanism-of-action. Mol Cell Proteomics. 2017;16:S54-S64 pubmed publisher
    ..We also showed that PfRab5b, which lacks C-terminal cysteines that are the only known site of Rab GTPase modification, is prenylated...
  22. Tall G, Barbieri M, Stahl P, Horazdovsky B. Ras-activated endocytosis is mediated by the Rab5 guanine nucleotide exchange activity of RIN1. Dev Cell. 2001;1:73-82 pubmed
    ..We conclude that Ras-activated endocytosis is facilitated, in part, by the ability of Ras to directly regulate the Rab5 nucleotide exchange activity of RIN1. ..
  23. Callaghan J, Nixon S, Bucci C, Toh B, Stenmark H. Direct interaction of EEA1 with Rab5b. Eur J Biochem. 1999;265:361-6 pubmed
    ..Sequencing of these clones revealed that they all contained the ORF of the small GTPase, Rab5b. Further two-hybrid analysis suggested that Rab5b also interacts with the N-terminus of EEA1 (EEA1-NT)...
  24. Kajiho H, Saito K, Tsujita K, Kontani K, Araki Y, Kurosu H, et al. RIN3: a novel Rab5 GEF interacting with amphiphysin II involved in the early endocytic pathway. J Cell Sci. 2003;116:4159-68 pubmed
    ..These results indicate that RIN3 biochemically characterized as the stimulator and stabilizer for GTP-Rab5 plays an important role in the transport pathway from plasma membrane to early endosomes. ..
  25. Bucci C, Chiariello M, Lattero D, Maiorano M, Bruni C. Interaction cloning and characterization of the cDNA encoding the human prenylated rab acceptor (PRA1). Biochem Biophys Res Commun. 1999;258:657-62 pubmed
    ..Analysis of mRNA expression shows a single abundant mRNA of about 0.8 kb ubiquitously expressed. Western blot analysis of the overexpressed protein shows a band of the expected size equally distributed between cytosol and membranes. ..
  26. Hakonarson H, Qu H, Bradfield J, Marchand L, Kim C, Glessner J, et al. A novel susceptibility locus for type 1 diabetes on Chr12q13 identified by a genome-wide association study. Diabetes. 2008;57:1143-6 pubmed publisher
    ..These two loci are identical to two loci identified by the whole-genome association study of the Wellcome Trust Case-Control Consortium, a parallel independent discovery that adds further support to the validity of the GWA approach. ..
  27. Yun H, Kim H, Ga I, Oh H, Ho D, Kim J, et al. An early endosome regulator, Rab5b, is an LRRK2 kinase substrate. J Biochem. 2015;157:485-95 pubmed publisher
    ..We showed previously that Rab5b, a small GTPase protein that regulates the motility and fusion of early endosomes, interacts with LRRK2 and co-..
  28. Bao S, Zhu J, Garvey W. Cloning of Rab GTPases expressed in human skeletal muscle: studies in insulin-resistant subjects. Horm Metab Res. 1998;30:656-62 pubmed
    ..Multiple Rab isoforms including Rab1A, Rab4A, Rab5B, Rab7, Rab8, Rab10, Rab12A, Rab13, Rab18, Rab21, and Rab22 mRNA were found to be expressed in human skeletal ..
  29. Bucci C, Lutcke A, Steele Mortimer O, Olkkonen V, Dupree P, Chiariello M, et al. Co-operative regulation of endocytosis by three Rab5 isoforms. FEBS Lett. 1995;366:65-71 pubmed
    ..Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c. The two proteins share with Rab5a all the structural features required for regulation of endocytosis...
  30. Jin Y, Birlea S, Fain P, Mailloux C, Riccardi S, Gowan K, et al. Common variants in FOXP1 are associated with generalized vitiligo. Nat Genet. 2010;42:576-8 pubmed publisher
    ..04x10(-8)) and with variants at 6q27 encompassing CCR6 (rs6902119, combined P=3.94x10(-7)). ..
  31. Tang X, Zhang Z, Hu D, Xu A, Zhou H, Sun L, et al. Association analyses identify three susceptibility Loci for vitiligo in the Chinese Han population. J Invest Dermatol. 2013;133:403-10 pubmed publisher
    ..They also highlight similarities and differences in the genetic basis of vitiligo in Chinese and Caucasian populations. ..
  32. Shi Y, Zhao H, Shi Y, Cao Y, Yang D, Li Z, et al. Genome-wide association study identifies eight new risk loci for polycystic ovary syndrome. Nat Genet. 2012;44:1020-5 pubmed publisher
    ..Other candidate genes were related to calcium signaling and endocytosis. Our findings provide new insight and direction for discovering the biological mechanisms of PCOS. ..
  33. Correia M, Moreiras H, Pereira F, Neto M, Festas T, Tarafder A, et al. Melanin Transferred to Keratinocytes Resides in Nondegradative Endocytic Compartments. J Invest Dermatol. 2018;138:637-646 pubmed publisher
    ..Furthermore, we found that the silencing of the early endocytic regulator Rab5b, but not the late endocytic regulators Rab7a or Rab9a, significantly impairs melanocore uptake by keratinocytes...
  34. Chou C, Hwang G, Hageman D, Han L, Agrawal P, Pisitkun T, et al. Identification of UT-A1 and AQP2 interacting proteins in rat inner medullary collecting duct. Am J Physiol Cell Physiol. 2017;:ajpcell.00082.2017 pubmed publisher
    ..Among the proteins unique to the AQP2 list were several Rab proteins (Rab1a, Rab2a, Rab5b, Rab5c, Rab7a, Rab11a, Rab11b, Rab14, Rab17) involved in membrane trafficking...
  35. Korenberg J, Chen X, Adams M, Venter J. Toward a cDNA map of the human genome. Genomics. 1995;29:364-70 pubmed
    ..This assignment provides a necessary first step in understanding the relationship of the genes to both acquired and inherited human diseases. ..
  36. Merithew E, Stone C, Eathiraj S, Lambright D. Determinants of Rab5 interaction with the N terminus of early endosome antigen 1. J Biol Chem. 2003;278:8494-500 pubmed
  37. Ebine K, Hirai M, Sakaguchi M, Yahata K, Kaneko O, Saito Nakano Y. Plasmodium Rab5b is secreted to the cytoplasmic face of the tubovesicular network in infected red blood cells together with N-acylated adenylate kinase 2. Malar J. 2016;15:323 pubmed publisher
    ..Plasmodium falciparum encodes three Rab5 isotypes, and one of these, Rab5b (PfRab5b), lacks the C-terminal modification but possesses the N-terminal myristoylation motif...
  38. Ho D, Jang J, Joe E, Son I, Seo H, Seol W. G2385R and I2020T Mutations Increase LRRK2 GTPase Activity. Biomed Res Int. 2016;2016:7917128 pubmed publisher
  39. Pereira Leal J, Strom M, Godfrey R, Seabra M. Structural determinants of Rab and Rab Escort Protein interaction: Rab family motifs define a conserved binding surface. Biochem Biophys Res Commun. 2003;301:92-7 pubmed
    ..These results support the hypothesis that Rabs bind REP via conserved RabF motifs and provide a molecular explanation for the preferential recognition of the GDP-bound conformation of Rab by REP. ..
  40. Hirota Y, Kuronita T, Fujita H, Tanaka Y. A role for Rab5 activity in the biogenesis of endosomal and lysosomal compartments. Biochem Biophys Res Commun. 2007;364:40-7 pubmed
    ..We show here that expression of GFP-tagged GTPase-deficient form of Rab5b (Rab5bQ79L) in NRK cells results in the sequential formation of three morphologically and functionally distinct ..
  41. Imai Y, Kobayashi Y, Inoshita T, Meng H, Arano T, Uemura K, et al. The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 2015;11:e1005503 pubmed publisher
    ..These effects are strengthened by the R1441G ROC domain-mutant of LRRK2. These findings suggest that the alteration of Notch signaling in mature neurons is a component of PD etiology linked to LRRK2. ..
  42. Chen P, Kong C, Su X, Stahl P. Rab5 isoforms differentially regulate the trafficking and degradation of epidermal growth factor receptors. J Biol Chem. 2009;284:30328-38 pubmed publisher
    ..282, 15294-15301), we hypothesize that the selective association of Rab5A and Rin1 contributes to the dominance of Rab5A in EGFR trafficking, whereas the other isoforms may have major functions unrelated to the EGFR degradation pathway. ..
  43. Louwers Y, Stolk L, Uitterlinden A, Laven J. Cross-ethnic meta-analysis of genetic variants for polycystic ovary syndrome. J Clin Endocrinol Metab. 2013;98:E2006-12 pubmed publisher
    ..Because ethnic background adds to phenotypic diversities in PCOS, it seems plausible that genetic variants associated with PCOS act differently in various ethnic populations...