Rab5

Summary

Gene Symbol: Rab5
Description: RAB5A, member RAS oncogene family
Alias: RAB5, ras-related protein Rab-5A, RAS-associated protein RAB5A
Species: human
Products:     Rab5

Top Publications

  1. Miaczynska M, Christoforidis S, Giner A, Shevchenko A, Uttenweiler Joseph S, Habermann B, et al. APPL proteins link Rab5 to nuclear signal transduction via an endosomal compartment. Cell. 2004;116:445-56 pubmed
    ..We report the identification of a pathway directly linking the small GTPase Rab5, a key regulator of endocytosis, to signal transduction and mitogenesis...
  2. Rosenfeld J, Moore R, Zimmer K, Alpizar Foster E, Dai W, Zarka M, et al. Lysosome proteins are redistributed during expression of a GTP-hydrolysis-defective rab5a. J Cell Sci. 2001;114:4499-508 pubmed
    ..These results suggest that expression of a GTPase-defective rab5a affects lysosome biogenesis by alteration of traffic between lysosomes and endosomes. ..
  3. Kauppi M, Simonsen A, Bremnes B, Vieira A, Callaghan J, Stenmark H, et al. The small GTPase Rab22 interacts with EEA1 and controls endosomal membrane trafficking. J Cell Sci. 2002;115:899-911 pubmed
    ..is a small GTPase that is expressed ubiquitously in mammalian tissues and displays the highest sequence homology to Rab5. In BHK-21 cells, overexpression of the wild-type Rab22a caused formation of abnormally large vacuole-like ..
  4. Stenmark H, Vitale G, Ullrich O, Zerial M. Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion. Cell. 1995;83:423-32 pubmed
    ..a novel 100 kDa coiled-coil protein, rabaptin-5, that specifically interacts with the GTP form of the small GTPase Rab5, a potent regulator of endocytic transport...
  5. Vitale G, Rybin V, Christoforidis S, Thornqvist P, McCaffrey M, Stenmark H, et al. Distinct Rab-binding domains mediate the interaction of Rabaptin-5 with GTP-bound Rab4 and Rab5. EMBO J. 1998;17:1941-51 pubmed
    Rabaptin-5 functions as an effector for the small GTPase Rab5, a regulator of endocytosis and early endosome fusion. We have searched for structural determinants that confer functional specificity on Rabaptin-5...
  6. Liu S, Chen X, Zheng H, Shi S, Li Y. Knockdown of Rab5a expression decreases cancer cell motility and invasion through integrin-mediated signaling pathway. J Biomed Sci. 2011;18:58 pubmed publisher
    ..Taken together, these data suggest that Rab5a functions in regulating the invasion phenotype, and we propose that this regulation may be via integrin-mediated signaling pathway in cervical cancer cells. ..
  7. Christoforidis S, Zerial M. Purification and identification of novel Rab effectors using affinity chromatography. Methods. 2000;20:403-10 pubmed
    ..the experimental system we have developed to biochemically purify and identify effectors of the small GTPase Rab5. The method, which is based on an affinity chromatography procedure, results in the large-scale purification of Rab ..
  8. Mallo G, Espina M, Smith A, Terebiznik M, Aleman A, Finlay B, et al. SopB promotes phosphatidylinositol 3-phosphate formation on Salmonella vacuoles by recruiting Rab5 and Vps34. J Cell Biol. 2008;182:741-52 pubmed publisher
    ..Instead, the phosphatase activity of SopB is required for Rab5 recruitment to the SCV...
  9. Schnatwinkel C, Christoforidis S, Lindsay M, Uttenweiler Joseph S, Wilm M, Parton R, et al. The Rab5 effector Rabankyrin-5 regulates and coordinates different endocytic mechanisms. PLoS Biol. 2004;2:E261 pubmed
    The small GTPase Rab5 is a key regulator of clathrin-mediated endocytosis...

More Information

Publications83

  1. Torres V, Mielgo A, Barbero S, Hsiao R, Wilkins J, Stupack D. Rab5 mediates caspase-8-promoted cell motility and metastasis. Mol Biol Cell. 2010;21:369-76 pubmed publisher
    ..The proteins responsible for integration of these pathways, however, have remained elusive. Here, we reveal that Rab5 regulates caspase-8-dependent signaling from integrins...
  2. Chen P, Kong C, Su X, Stahl P. Rab5 isoforms differentially regulate the trafficking and degradation of epidermal growth factor receptors. J Biol Chem. 2009;284:30328-38 pubmed publisher
    ..We focused our work on Rab5, a monomeric GTPase shown to participate in early stages of the endocytic pathway...
  3. Kajiho H, Saito K, Tsujita K, Kontani K, Araki Y, Kurosu H, et al. RIN3: a novel Rab5 GEF interacting with amphiphysin II involved in the early endocytic pathway. J Cell Sci. 2003;116:4159-68 pubmed
    The small GTPase Rab5, which cycles between active (GTP-bound) and inactive (GDP-bound) states, plays essential roles in membrane budding and trafficking in the early endocytic pathway...
  4. Saito K, Murai J, Kajiho H, Kontani K, Kurosu H, Katada T. A novel binding protein composed of homophilic tetramer exhibits unique properties for the small GTPase Rab5. J Biol Chem. 2002;277:3412-8 pubmed
    ..Among them, Rab5 is involved in early endocytic pathway, and several Rab5-binding proteins have been identified as regulators or ..
  5. Pellinen T, Arjonen A, Vuoriluoto K, Kallio K, Fransen J, Ivaska J. Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins. J Cell Biol. 2006;173:767-80 pubmed
    ..Rab21 (and Rab5) associate with the cytoplasmic domains of alpha-integrin chains, and their expression influences the endo/exocytic ..
  6. Mao X, Kikani C, Riojas R, Langlais P, Wang L, Ramos F, et al. APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function. Nat Cell Biol. 2006;8:516-23 pubmed
    ..Adiponectin stimulates the interaction between APPL1 and Rab5 (a small GTPase) interaction, leading to increased GLUT4 membrane translocation...
  7. Eathiraj S, Pan X, Ritacco C, Lambright D. Structural basis of family-wide Rab GTPase recognition by rabenosyn-5. Nature. 2005;436:415-9 pubmed
  8. Zhang X, He X, Fu X, Chang Z. Varp is a Rab21 guanine nucleotide exchange factor and regulates endosome dynamics. J Cell Sci. 2006;119:1053-62 pubmed
    The small GTPases Rab5 and Rab21 are closely related, and play essential roles in endocytic trafficking. Rab5 is regulated by VPS9-domain-containing guanine nucleotide exchange factors...
  9. Leclerc E, Gazeilles L, Serre G, Guerrin M, Jonca N. The ubiquitous dermokine delta activates Rab5 function in the early endocytic pathway. PLoS ONE. 2011;6:e17816 pubmed publisher
    ..To get an insight into Dmkn? function, we performed yeast two-hybrid screening and identified the small GTPases Rab5 as partners for Dmkn?...
  10. Tall G, Barbieri M, Stahl P, Horazdovsky B. Ras-activated endocytosis is mediated by the Rab5 guanine nucleotide exchange activity of RIN1. Dev Cell. 2001;1:73-82 pubmed
    ..RIN1 is also shown to stimulate Rab5 guanine nucleotide exchange, Rab5A-dependent endosome fusion, and EGF receptor-mediated endocytosis...
  11. Serio G, Margaria V, Jensen S, Oldani A, Bartek J, Bussolino F, et al. Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores. Proc Natl Acad Sci U S A. 2011;108:17337-42 pubmed publisher
    b>Rab5 is a small GTPase known to regulate vesicular trafficking during interphase. Here, we show that Rab5 also plays an unexpected role during mitotic progression...
  12. Lanzetti L, Rybin V, Malabarba M, Christoforidis S, Scita G, Zerial M, et al. The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5. Nature. 2000;408:374-7 pubmed
    ..Through its src homology-3 domain, Eps8 interacts with RN-tre. We show that RN-tre is a Rab5 GTPase-activating protein, whose activity is regulated by the EGFR...
  13. Macovei A, Petrareanu C, Lazar C, Florian P, Branza Nichita N. Regulation of hepatitis B virus infection by Rab5, Rab7, and the endolysosomal compartment. J Virol. 2013;87:6415-27 pubmed publisher
    ..The results showed that HBV infection strongly depends on Rab5 and Rab7 expression, indicating that HBV transport from early to mature endosomes is required for a step in the ..
  14. Wiesner C, El Azzouzi K, Linder S. A specific subset of RabGTPases controls cell surface exposure of MT1-MMP, extracellular matrix degradation and three-dimensional invasion of macrophages. J Cell Sci. 2013;126:2820-33 pubmed publisher
  15. Callaghan J, Nixon S, Bucci C, Toh B, Stenmark H. Direct interaction of EEA1 with Rab5b. Eur J Biochem. 1999;265:361-6 pubmed
    ..These data provide evidence that EEA1 interacts with both Rab5a and Rab5b, and that the GTPase activities of the two proteins are differentially regulated in vivo. ..
  16. Mendoza P, Ortiz R, Diaz J, Quest A, Leyton L, Stupack D, et al. Rab5 activation promotes focal adhesion disassembly, migration and invasiveness in tumor cells. J Cell Sci. 2013;126:3835-47 pubmed publisher
    ..Indeed, the small GTPase Rab5, a crucial regulator of early endosome dynamics, promotes cell migration in vitro and in vivo...
  17. Diaz J, Mendoza P, Ortiz R, Diaz N, Leyton L, Stupack D, et al. Rab5 is required in metastatic cancer cells for Caveolin-1-enhanced Rac1 activation, migration and invasion. J Cell Sci. 2014;127:2401-6 pubmed publisher
    b>Rab5 is a small GTPase that regulates early endosome trafficking and other cellular processes, including cell adhesion and migration...
  18. Zahraoui A, Touchot N, Chardin P, Tavitian A. The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion. J Biol Chem. 1989;264:12394-401 pubmed
    Seven cDNA clones corresponding to the rab1, rab2, rab3A, rab3B, rab4, rab5, and rab6 genes were isolated from a human pheochromocytoma cDNA library...
  19. Torres V, Mielgo A, Barilà D, Anderson D, Stupack D. Caspase 8 promotes peripheral localization and activation of Rab5. J Biol Chem. 2008;283:36280-9 pubmed publisher
    ..Here we demonstrate the regulation of the small GTPase Rab5, which mediates early endosome formation, homotypic fusion, and maturation by caspase 8...
  20. Blümer J, Rey J, Dehmelt L, Mazel T, Wu Y, Bastiaens P, et al. RabGEFs are a major determinant for specific Rab membrane targeting. J Cell Biol. 2013;200:287-300 pubmed publisher
    ..Therefore, RabGEFs are major determinants for specific Rab membrane targeting...
  21. Fuchs E, Haas A, Spooner R, Yoshimura S, Lord J, Barr F. Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways. J Cell Biol. 2007;177:1133-43 pubmed
    ..We show that Rab43 is the target of RN-tre and is required for Shiga toxin uptake. In contrast, RabGAP-5, a Rab5 GAP, was unique among the GAPs tested and reduced the uptake of EGF but not Shiga toxin...
  22. Hyvola N, Diao A, McKenzie E, Skippen A, Cockcroft S, Lowe M. Membrane targeting and activation of the Lowe syndrome protein OCRL1 by rab GTPases. EMBO J. 2006;25:3750-61 pubmed
    ..Strongest interaction is seen with Golgi-associated rab1 and rab6 and endosomal rab5. Point mutants defective in rab binding fail to target to the Golgi apparatus and endosomes, strongly suggesting ..
  23. Lee J, Kim D, Kim D, Simborio H, Min W, Lee H, et al. Interplay between clathrin and Rab5 controls the early phagocytic trafficking and intracellular survival of Brucella abortus within HeLa cells. J Biol Chem. 2013;288:28049-57 pubmed publisher
    ..abortus-containing vacuoles with clathrin and Rab5 but not lysosome-associated membrane protein 1 (LAMP-1)...
  24. Chamberlain M, Berry T, Pastor M, Anderson D. The p85alpha subunit of phosphatidylinositol 3'-kinase binds to and stimulates the GTPase activity of Rab proteins. J Biol Chem. 2004;279:48607-14 pubmed
    b>Rab5 and Rab4 are small monomeric GTPases localized on early endosomes and function in vesicle fusion events...
  25. Zhu G, Zhai P, Liu J, Terzyan S, Li G, Zhang X. Structural basis of Rab5-Rabaptin5 interaction in endocytosis. Nat Struct Mol Biol. 2004;11:975-83 pubmed
    b>Rab5 is a small GTPase that regulates early endosome fusion. We present here the crystal structure of the Rab5 GTPase domain in complex with a GTP analog and the C-terminal domain of effector Rabaptin5...
  26. Galperin E, Sorkin A. Visualization of Rab5 activity in living cells by FRET microscopy and influence of plasma-membrane-targeted Rab5 on clathrin-dependent endocytosis. J Cell Sci. 2003;116:4799-810 pubmed
    b>Rab5 is a small GTPase that controls endocytosis and early endosome dynamics. To visualize active, GTP-loaded Rab5 in living cells, we developed molecular sensors consisting of the Rab5-binding fragments of Rabaptin5 or EEA...
  27. Bucci C, Parton R, Mather I, Stunnenberg H, Simons K, Hoflack B, et al. The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway. Cell. 1992;70:715-28 pubmed
    We have investigated the in vivo functional role of rab5, a small GTPase associated with the plasma membrane and early endosomes...
  28. Zhu G, Liu J, Terzyan S, Zhai P, Li G, Zhang X. High resolution crystal structures of human Rab5a and five mutants with substitutions in the catalytically important phosphate-binding loop. J Biol Chem. 2003;278:2452-60 pubmed
  29. Simonsen A, Lippe R, Christoforidis S, Gaullier J, Brech A, Callaghan J, et al. EEA1 links PI(3)K function to Rab5 regulation of endosome fusion. Nature. 1998;394:494-8 pubmed
    ..between early endosomes requires phosphatidylinositol-3-OH kinase (PI(3)K) activity as well as the small GTPase Rab5. Excess Rab5-GTP complex restores endosome fusion when PI(3)K is inhibited...
  30. Seachrist J, Laporte S, Dale L, Babwah A, Caron M, Anborgh P, et al. Rab5 association with the angiotensin II type 1A receptor promotes Rab5 GTP binding and vesicular fusion. J Biol Chem. 2002;277:679-85 pubmed
  31. Hoffenberg S, Liu X, Nikolova L, Hall H, Dai W, Baughn R, et al. A novel membrane-anchored Rab5 interacting protein required for homotypic endosome fusion. J Biol Chem. 2000;275:24661-9 pubmed
    The ras-related GTPase rab5 is rate-limiting for homotypic early endosome fusion. We used a yeast two-hybrid screen to identify a rab5 interacting protein, rab5ip...
  32. Nielsen E, Christoforidis S, Uttenweiler Joseph S, Miaczynska M, Dewitte F, Wilm M, et al. Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain. J Cell Biol. 2000;151:601-12 pubmed
    b>Rab5 regulates endocytic membrane traffic by specifically recruiting cytosolic effector proteins to their site of action on early endosomal membranes. We have characterized a new Rab5 effector complex involved in endosomal fusion events...
  33. SILVA P, Mendoza P, Rivas S, Diaz J, Moraga C, Quest A, et al. Hypoxia promotes Rab5 activation, leading to tumor cell migration, invasion and metastasis. Oncotarget. 2016;7:29548-62 pubmed publisher
    ..The early endosome protein Rab5 is a small GTPase that promotes integrin trafficking, focal adhesion turnover, Rac1 activation, tumor cell ..
  34. Hsiao J, Chu L, Lo Y, Lee S, Chen T, Huang C, et al. Intracellular Transport of Vaccinia Virus in HeLa Cells Requires WASH-VPEF/FAM21-Retromer Complexes and Recycling Molecules Rab11 and Rab22. J Virol. 2015;89:8365-82 pubmed publisher
    ..vesicles are internalized into phosphatidylinositol 3-phosphate positive macropinosomes, which are then fused with Rab5-positive early endosomes...
  35. Masuda E, Luo Y, Young C, Shen M, Rossi A, Huang B, et al. Rab37 is a novel mast cell specific GTPase localized to secretory granules. FEBS Lett. 2000;470:61-4 pubmed
    ..These data suggest that Rab37 may play an important role in mast cell degranulation making this protein a potentially important target for therapeutic intervention in the treatment of allergy. ..
  36. Podhajska A, Musso A, Trancikova A, Stafa K, Moser R, Sonnay S, et al. Common pathogenic effects of missense mutations in the P-type ATPase ATP13A2 (PARK9) associated with early-onset parkinsonism. PLoS ONE. 2012;7:e39942 pubmed publisher
    ..Collectively, our study provides support for common loss-of-function effects of homozygous and heterozygous missense mutations in ATP13A2 associated with early-onset forms of parkinsonism. ..
  37. Cong B, Ohsawa S, Igaki T. JNK and Yorkie drive tumor progression by generating polyploid giant cells in Drosophila. Oncogene. 2018;37:3088-3097 pubmed publisher
    ..We previously noticed in Drosophila imaginal epithelium that cells mutant for the endocytic gene rab5 exhibit enlarged nuclei...
  38. Warrington S, Strutt H, Fisher K, Strutt D. A Dual Function for Prickle in Regulating Frizzled Stability during Feedback-Dependent Amplification of Planar Polarity. Curr Biol. 2017;27:2784-2797.e3 pubmed publisher
    ..This destabilizing activity is dependent on the presence of Dishevelled and blocked in the absence of Dynamin and Rab5 activity, suggesting an endocytic mechanism...
  39. Jacobsen S, Ammendrup Johnsen I, Jansen A, Gether U, Madsen K, Bräuner Osborne H. The GPRC6A receptor displays constitutive internalization and sorting to the slow recycling pathway. J Biol Chem. 2017;292:6910-6926 pubmed publisher
    ..Internalized GPRC6A was mainly co-localized with the early endosome marker Rab5 and the long loop recycling endosome marker Rab11 and to a much lesser extent with the late endosome marker Rab7...
  40. Bomberger J, Barnaby R, Stanton B. The deubiquitinating enzyme USP10 regulates the post-endocytic sorting of cystic fibrosis transmembrane conductance regulator in airway epithelial cells. J Biol Chem. 2009;284:18778-89 pubmed publisher
    ..These studies demonstrate a novel function for USP10 in facilitating the deubiquitination of CFTR in early endosomes and thereby enhancing the endocytic recycling of CFTR. ..
  41. Jopling H, Odell A, Hooper N, Zachary I, Walker J, Ponnambalam S. Rab GTPase regulation of VEGFR2 trafficking and signaling in endothelial cells. Arterioscler Thromb Vasc Biol. 2009;29:1119-24 pubmed publisher
    ..Our data suggest that VEGFR2-mediated regulation of endothelial function is dependent on different but specific Rab-mediated GTP hydrolysis activity required for endosomal trafficking. ..
  42. Panigrahi G, Praharaj P, Peak T, Long J, Singh R, Rhim J, et al. Hypoxia-induced exosome secretion promotes survival of African-American and Caucasian prostate cancer cells. Sci Rep. 2018;8:3853 pubmed publisher
    ..Further, under hypoxia, Rab5 (a biomarker for early endosome) was clustered in perinuclear region; and CD63 (a biomarker for exosomes and ..
  43. Seet L, Liu N, Hanson B, Hong W. Endofin recruits TOM1 to endosomes. J Biol Chem. 2004;279:4670-9 pubmed
    ..A marked recruitment of TOM1 to endosomes was observed in cells overexpressing endofin or its carboxyl-terminal fragment, indicating TOM1 to be an effector for endofin and suggesting a possible role for TOM1 in endosomal trafficking. ..
  44. Sheng Y, Li J, Zou C, Wang S, Cao Y, Zhang J, et al. Downregulation of miR-101-3p by hepatitis B virus promotes proliferation and migration of hepatocellular carcinoma cells by targeting Rab5a. Arch Virol. 2014;159:2397-410 pubmed publisher
    ..This provides new insights into the mechanisms of HBV-related HCC pathogenesis. ..
  45. Tam S, Lilla J, Chen C, Kalesnikoff J, Tsai M. RabGEF1/Rabex-5 Regulates TrkA-Mediated Neurite Outgrowth and NMDA-Induced Signaling Activation in NGF-Differentiated PC12 Cells. PLoS ONE. 2015;10:e0142935 pubmed publisher
    ..RabGEF1 (also known as Rabex-5) is a guanine nucleotide exchange factor for Rab5, which regulates early endosome fusion and vesicular trafficking in endocytic pathways...
  46. Ali N, Jurczyluk J, Shay G, Tnimov Z, Alexandrov K, Munoz M, et al. A highly sensitive prenylation assay reveals in vivo effects of bisphosphonate drug on the Rab prenylome of macrophages outside the skeleton. Small Gtpases. 2015;6:202-11 pubmed publisher
    ..a >7-fold increase in the unprenylated form of Rab proteins associated with the endophagosome pathway (Rab1, Rab5, Rab6, Rab7, Rab11, Rab14 and Rab21)...
  47. Li Y, Yang W, Cheng L, Lin C, Ho Y, Lin P, et al. Novel transport function of adherens junction revealed by live imaging in Drosophila. Biochem Biophys Res Commun. 2015;463:686-92 pubmed publisher
    ..in the epithelial cells of Drosophila embryos, we demonstrate that Echinoid vesicles co-localize and move with Rab5-or Rab11-positive endosomes...
  48. Verma J, Rastogi R, Mukhopadhyay A. Leishmania donovani resides in modified early endosomes by upregulating Rab5a expression via the downregulation of miR-494. PLoS Pathog. 2017;13:e1006459 pubmed publisher
    ..These results provide the first mechanistic insights of parasite-mediated remodeling of endo-lysosomal trafficking to reside in a specialized early endocytic compartment. ..
  49. Adams A, Wayne Vogl A. High Resolution Localization of Rab5, EEA1, and Nectin-3 to Tubulobulbar Complexes in the Rat Testis. Anat Rec (Hoboken). 2017;300:1160-1170 pubmed publisher
    ..Previous immunofluorescence studies have demonstrated that markers for early endosomes (Rab5 and EEA1) are associated with tubulobulbar complexes and are localized at or near the ends of the structures...
  50. Shi B, Liu C, Zhou J, Wang S, Gao Z, Zhang X, et al. Entry of Classical Swine Fever Virus into PK-15 Cells via a pH-, Dynamin-, and Cholesterol-Dependent, Clathrin-Mediated Endocytic Pathway That Requires Rab5 and Rab7. J Virol. 2016;90:9194-208 pubmed publisher
    ..Expression of DN Rab5 and Rab7 mutants, but not the DN Rab11 mutant, significantly inhibited CSFV replication...
  51. Meehan T, Joudi T, Timmons A, Taylor J, Habib C, Peterson J, et al. Components of the Engulfment Machinery Have Distinct Roles in Corpse Processing. PLoS ONE. 2016;11:e0158217 pubmed publisher
    ..show that engulfed material is processed using the canonical corpse processing pathway involving the small GTPases Rab5 and Rab7...
  52. Boothe T, Lim G, Cen H, Skovsø S, Piske M, Li S, et al. Inter-domain tagging implicates caveolin-1 in insulin receptor trafficking and Erk signaling bias in pancreatic beta-cells. Mol Metab. 2016;5:366-378 pubmed publisher
  53. Liu O, Grant B. Basolateral Endocytic Recycling Requires RAB-10 and AMPH-1 Mediated Recruitment of RAB-5 GAP TBC-2 to Endosomes. PLoS Genet. 2015;11:e1005514 pubmed publisher
    The small GTPase RAB-5/Rab5 is a master regulator of the early endosome, required for a myriad of coordinated activities, including the degradation and recycling of internalized cargo...
  54. Song C, Lu B, Shi Z, Yu Y, Li Y, Li P. [The effects of mutant site G81R of RAB5A on RAB5A function]. Yi Chuan Xue Bao. 2003;30:967-72 pubmed
    ..The antisense RNA of RAB5A G81R effectively blocked the expression of RAB5A in Anip973. Cell cycle was lengthened by blocking or reducing expression of RAB5A G81R. ..
  55. Dirk B, Jacob R, Johnson A, Pawlak E, Cavanagh P, Van Nynatten L, et al. Viral bimolecular fluorescence complementation: a novel tool to study intracellular vesicular trafficking pathways. PLoS ONE. 2015;10:e0125619 pubmed publisher
    ..In summary, viral BiFC is a unique tool designed to analyze the interaction between Nef and host cellular proteins by mapping the sub-cellular locations of their interactions during viral infection. ..
  56. Mottola G. The complexity of Rab5 to Rab7 transition guarantees specificity of pathogen subversion mechanisms. Front Cell Infect Microbiol. 2014;4:180 pubmed publisher
  57. Sewduth R, Jaspard Vinassa B, Peghaire C, Guillabert A, Franzl N, Larrieu Lahargue F, et al. The ubiquitin ligase PDZRN3 is required for vascular morphogenesis through Wnt/planar cell polarity signalling. Nat Commun. 2014;5:4832 pubmed publisher
    ..These results highlight the role of PDZRN3 to direct Wnt PCP signalling, and broadly implicate this pathway in the planar orientation and highly branched organization of vascular plexuses. ..
  58. Allonby O, El Zawily A, Freywald T, Mousseau D, Chlan J, Anderson D, et al. Ligand stimulation induces clathrin- and Rab5-dependent downregulation of the kinase-dead EphB6 receptor preceded by the disruption of EphB6-Hsp90 interaction. Cell Signal. 2014;26:2645-57 pubmed publisher
    ..Their targeting for lysosomal degradation relies on the activity of an early endosome regulator, the Rab5 GTPase, as this process is inhibited in the presence of a Rab5 dominant-negative mutant...
  59. Weir D, Laing E, Smith I, Wang L, Broder C. Host cell virus entry mediated by Australian bat lyssavirus G envelope glycoprotein occurs through a clathrin-mediated endocytic pathway that requires actin and Rab5. Virol J. 2014;11:40 pubmed publisher
    ..Over expression of dominant negative mutants of Eps15 and Rab5 also significantly reduced ABLV G-mediated entry into HEK293T cells...
  60. Farnsworth C, Seabra M, Ericsson L, Gelb M, Glomset J. Rab geranylgeranyl transferase catalyzes the geranylgeranylation of adjacent cysteines in the small GTPases Rab1A, Rab3A, and Rab5A. Proc Natl Acad Sci U S A. 1994;91:11963-7 pubmed
  61. Perskvist N, Roberg K, Kulyté A, Stendahl O. Rab5a GTPase regulates fusion between pathogen-containing phagosomes and cytoplasmic organelles in human neutrophils. J Cell Sci. 2002;115:1321-30 pubmed
    ..These results also identified Rab5a as a key regulator of phagolysosome maturation in human neutrophils. ..
  62. Kumar B, Dutta D, Iqbal J, Ansari M, Roy A, Chikoti L, et al. ESCRT-I Protein Tsg101 Plays a Role in the Post-macropinocytic Trafficking and Infection of Endothelial Cells by Kaposi's Sarcoma-Associated Herpesvirus. PLoS Pathog. 2016;12:e1005960 pubmed publisher
    ..Tsg101 was also associated with early (Rab5) and late endosomal (Rab7) stages of KSHV intracellular trafficking, and CHMP5 (ESCRT-III) was also associated with ..
  63. Aikawa Y, Hirakawa H, Lee S. Spatiotemporal regulation of the ubiquitinated cargo-binding activity of Rabex-5 in the endocytic pathway. J Biol Chem. 2012;287:40586-97 pubmed publisher
    ..Previously, we reported the interaction of Rabex-5, a UBP and guanine nucleotide exchange factor (GEF) for Rab5, with ubiquitinated neural cell adhesion molecule L1, via its motif interacting with Ub (MIU) domain...
  64. Laifenfeld D, Patzek L, McPhie D, Chen Y, Levites Y, Cataldo A, et al. Rab5 mediates an amyloid precursor protein signaling pathway that leads to apoptosis. J Neurosci. 2007;27:7141-53 pubmed
    ..Levels of both APP-BP1 and Rab5 are elevated in early endosomes in cortical embryonic neurons expressing APP(V642I) or APP-BP1, in cultured skin ..
  65. Tang Y, Geng Q, Chen D, Zhao S, Liu X, Wang Z. Germline Proliferation Is Regulated by Somatic Endocytic Genes via JNK and BMP Signaling in Drosophila. Genetics. 2017;206:189-197 pubmed publisher
    ..In an EMS mutagenesis screen, we identified Rab5, a key factor in endocytosis, for its nonautonomous role in germline proliferation...
  66. Edler E, Stein M. Recognition and stabilization of geranylgeranylated human Rab5 by the GDP Dissociation Inhibitor (GDI). Small Gtpases. 2017;:1-16 pubmed publisher
    The small GTPase Rab5 is the key regulator of early endosomal fusion. It is post-translationally modified by covalent attachment of two geranylgeranyl (GG) chains to adjacent cysteine residues of the C-terminal hypervariable region (HVR)...
  67. Che Y, Zhang Y, Li M, Li X, Zhang L. In vitro and in vivo effect of PD-1/PD-L1 blockade on microglia/macrophage activation and T cell subset balance in cryptococcal meningitis. J Cell Biochem. 2017;: pubmed publisher
    ..And the expression of Rab5 and Rab11 were detected using an immunofluorescence assay...
  68. Seaman M. Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer. J Cell Biol. 2004;165:111-22 pubmed
    ..The data presented here support the hypothesis that retromer performs a selective function in endosome-to-Golgi transport, mediating retrieval of the CI-MPR, but not furin. ..
  69. Muriel O, Scott C, Larios J, Mercier V, Gruenberg J. In Vitro Polymerization of F-actin on Early Endosomes. J Vis Exp. 2017;: pubmed publisher
    ..fractions are prepared by floatation in sucrose gradients from cells expressing the early endosomal protein GFP-RAB5. Cytosolic fractions are prepared from separate batches of cells...
  70. Imai Y, Kobayashi Y, Inoshita T, Meng H, Arano T, Uemura K, et al. The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 2015;11:e1005503 pubmed publisher
    ..These effects are strengthened by the R1441G ROC domain-mutant of LRRK2. These findings suggest that the alteration of Notch signaling in mature neurons is a component of PD etiology linked to LRRK2. ..
  71. Kim A, Davis R, Higuchi M. Intracellular oxygen determined by respiration regulates localization of Ras and prenylated proteins. Cell Death Dis. 2015;6:e1825 pubmed publisher
    ..These results demonstrate that intracellular oxygen concentration regulates the localization and activation of prenylated proteins. ..
  72. Zhao Z, Sagare A, Ma Q, Halliday M, Kong P, Kisler K, et al. Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance. Nat Neurosci. 2015;18:978-87 pubmed publisher
    ..low density lipoprotein receptor related protein-1, a key Aβ clearance receptor, and guided Aβ trafficking to Rab5 and Rab11, leading to Aβ endothelial transcytosis and clearance...
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    ..A similar binding was seen to rab1 but not to rab6, both Golgi rabs. In contrast, rab5 was as expected, shown to bind rabaptin-5 and rabex-5 as well as the endosomal effector protein EEA1 but not GM130...
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    ..Thus these results indicate that ClC-5 is present in the small intestine and colon of rats and suggest that it plays a role in the endocytotic pathways of intestinal cells...