Rab3A

Summary

Gene Symbol: Rab3A
Description: RAB3A, member RAS oncogene family
Alias: ras-related protein Rab-3A, RAS-associated protein RAB3A
Species: human
Products:     Rab3A

Top Publications

  1. Nagano F, Kawabe H, Nakanishi H, Shinohara M, Deguchi Tawarada M, Takeuchi M, et al. Rabconnectin-3, a novel protein that binds both GDP/GTP exchange protein and GTPase-activating protein for Rab3 small G protein family. J Biol Chem. 2002;277:9629-32 pubmed
    b>Rab3A, a member of the Rab3 small G protein family, regulates Ca(2+)-dependent exocytosis of neurotransmitter. The cyclical activation and inactivation of Rab3A are essential for the Rab3A action in exocytosis...
  2. Geppert M, Goda Y, Stevens C, Sudhof T. The small GTP-binding protein Rab3A regulates a late step in synaptic vesicle fusion. Nature. 1997;387:810-4 pubmed
    ..In brain, the Rab protein Rab3A is specific to synaptic vesicles, whose exocytosis can be monitored with submillisecond resolution by following ..
  3. Betz A, Thakur P, Junge H, Ashery U, Rhee J, Scheuss V, et al. Functional interaction of the active zone proteins Munc13-1 and RIM1 in synaptic vesicle priming. Neuron. 2001;30:183-96 pubmed
    ..The Munc13-1/RIM1 interaction may create a functional link between synaptic vesicle tethering and priming, or it may regulate the priming reaction itself, thereby determining the number of fusion-competent vesicles. ..
  4. Luo H, Saiardi A, Nagata E, Ye K, Yu H, Jung T, et al. GRAB: a physiologic guanine nucleotide exchange factor for Rab3A, which interacts with inositol hexakisphosphate kinase. Neuron. 2001;31:439-51 pubmed
    ..the identification, cloning, and characterization of a novel protein, GRAB (guanine nucleotide exchange factor for Rab3A), which interacts with both InsP6K1 and Rab3A, a Ras-like GTPase that regulates synaptic vesicle exocytosis...
  5. Yi Z, Yokota H, Torii S, Aoki T, Hosaka M, Zhao S, et al. The Rab27a/granuphilin complex regulates the exocytosis of insulin-containing dense-core granules. Mol Cell Biol. 2002;22:1858-67 pubmed
    ..Although Rab3a, another exocytotic Rab protein, has some similarities with Rab27a in primary sequence, intracellular distribution,..
  6. Wollert T, Patel A, Lee Y, Provance D, Vought V, Cosgrove M, et al. Myosin5a tail associates directly with Rab3A-containing compartments in neurons. J Biol Chem. 2011;286:14352-61 pubmed publisher
    ..A potential class of binding partners are Rab GTPases and Rab3A is known to associate with SVs and is involved in SV trafficking...
  7. Wang H, Wang R, Xu S, Lakshmana M. RanBP9 overexpression accelerates loss of pre and postsynaptic proteins in the APΔE9 transgenic mouse brain. PLoS ONE. 2014;9:e85484 pubmed publisher
    ..Specifically, at 5-months of age, rab3A was reduced in the triple transgenic mice only in the cortex by 25% (p<0...
  8. Sedej S, Klemen M, Schlüter O, Rupnik M. Rab3a is critical for trapping alpha-MSH granules in the high Ca²⁺-affinity pool by preventing constitutive exocytosis. PLoS ONE. 2013;8:e78883 pubmed publisher
    b>Rab3a is a small GTPase of the Rab3 subfamily that acts during late stages of Ca²⁺-regulated exocytosis. Previous functional analysis in pituitary melanotrophs described Rab3a as a positive regulator of Ca²⁺-dependent exocytosis...
  9. Tata B, Huijbregts L, Jacquier S, Csaba Z, Genin E, Meyer V, et al. Haploinsufficiency of Dmxl2, encoding a synaptic protein, causes infertility associated with a loss of GnRH neurons in mouse. PLoS Biol. 2014;12:e1001952 pubmed publisher
    ..which has been identified as a putative scaffold protein for Rab3-GAP and Rab3-GEP, two regulators of the GTPase Rab3a. We found that rabconnectin-3α was expressed in exocytosis vesicles in gonadotropin-releasing hormone (GnRH) ..

More Information

Publications101 found, 100 shown here

  1. Ren H, Xu Z, Guo W, Deng Z, Yu X. Rab3IP interacts with SSX2 and enhances the invasiveness of gastric cancer cells. Biochem Biophys Res Commun. 2018;503:2563-2568 pubmed publisher
    b>RAB3A interacting protein (Rab3IP) has been determined to be involved in cancer progression; however, its expression pattern and function in gastric cancer remain unknown...
  2. Yasuda T, Shibasaki T, Minami K, Takahashi H, Mizoguchi A, Uriu Y, et al. Rim2alpha determines docking and priming states in insulin granule exocytosis. Cell Metab. 2010;12:117-29 pubmed publisher
    ..We found that interaction of Rim2alpha and Rab3A is required for docking, which is considered a brake on fusion events, and that docking is necessary for K(+)-..
  3. Yuan Q, Zhou J, Teng L, Liu C, Guo J, Liu L, et al. Intrauterine growth retardation leads to the functional change of insulin secretion in the newborn rats. Horm Metab Res. 2010;42:491-5 pubmed publisher
    ..of insulin and PDX-1, the expression of genes related to insulin exocytosis such as munc13-1, vamp-2, syntaxin1a, rab3a were reduced significantly in IUGR newborn rats. IUGR animals were glucose-intolerant...
  4. Zhou H, Kang Y, Shi Z, Lu L, Li X, Chu T, et al. Identification of differentially expressed proteins in rats with spinal cord injury during the transitional phase using an iTRAQ-based quantitative analysis. Gene. 2018;677:66-76 pubmed publisher
    ..Alb, Calm1, Vim, Apoe, Syp, P4hb, Cd68, Eef1a2, Rab3a and Lgals3 were the top 10 high-degree core nodes...
  5. Quevedo M, Bustos M, Masone D, Roggero C, Bustos D, Tomes C. Grab recruitment by Rab27A-Rabphilin3a triggers Rab3A activation in human sperm exocytosis. Biochim Biophys Acta Mol Cell Res. 2018;1866:612-622 pubmed publisher
    ..indicate that Rabphilin3a behaves as a Rab27 effector during the AR and that GRAB exhibits GEF activity toward Rab3A. Recombinant, active Rab27A pulls down Rabphilin3a and GRAB from human sperm extracts...
  6. Regazzi R, Sasaki T, Takahashi K, Jonas J, Volker C, Stock J, et al. Prenylcysteine analogs mimicking the C-terminus of GTP-binding proteins stimulate exocytosis from permeabilized HIT-T15 cells: comparison with the effect of Rab3AL peptide. Biochim Biophys Acta. 1995;1268:269-78 pubmed
    ..The binding of G-proteins to Rab/GDP-dissociation inhibitor, Rab3A/GTPase activating protein or rabphilin-3A was not affected by the prenylcysteine analogs...
  7. Arora D, Syed I, Machhadieh B, McKenna C, Kowluru A. Rab-geranylgeranyl transferase regulates glucose-stimulated insulin secretion from pancreatic β cells. Islets. 2012;4:354-8 pubmed publisher
    ..g., Cdc42, Rac1, Arf6 and Rab3A and Rab27A) in islet β-cell function including glucose-stimulated insulin secretion (GSIS)...
  8. Huang C, Chiu T, Lee T, Hsieh H, Lin C, Kao L. Soluble α-synuclein facilitates priming and fusion by releasing Ca2+ from the thapsigargin-sensitive Ca2+ pool in PC12 cells. J Cell Sci. 2018;131: pubmed publisher
    ..and explored the molecular mechanism by which α-synuclein interacts with regulatory proteins, including Rab3A, Munc13-1 (also known as Unc13a) and Munc18-1 (also known as STXBP1), in order to regulate exocytosis...
  9. Wu W, Zheng X, Wang J, Yang T, Dai W, Song S, et al. O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma. Cell Death Dis. 2018;9:970 pubmed publisher
    b>Rab3A is a small Ras-like GTPase critical for membrane traffic. Although the functions of Rab3A have been reported in several cancers, the roles of Rab3A in hepatocellular carcinoma (HCC) have never been determined...
  10. Gitler A, Bevis B, Shorter J, Strathearn K, Hamamichi S, Su L, et al. The Parkinson's disease protein alpha-synuclein disrupts cellular Rab homeostasis. Proc Natl Acad Sci U S A. 2008;105:145-50 pubmed
    ..Other Rabs did not ameliorate alpha-syn toxicity in yeast, but RAB3A, which is highly expressed in neurons and localized to presynaptic termini, and RAB8A, which is localized to post-..
  11. Ljubicic S, Bezzi P, Brajkovic S, Nesca V, Guay C, Ohbayashi N, et al. The GTPase Rab37 Participates in the Control of Insulin Exocytosis. PLoS ONE. 2013;8:e68255 pubmed publisher
    Rab37 belongs to a subclass of Rab GTPases regulating exocytosis, including also Rab3a and Rab27a. Proteomic studies indicate that Rab37 is associated with insulin-containing large dense core granules of pancreatic β-cells...
  12. Löhner M, Babai N, Müller T, Gierke K, Atorf J, Joachimsthaler A, et al. Analysis of RIM Expression and Function at Mouse Photoreceptor Ribbon Synapses. J Neurosci. 2017;37:7848-7863 pubmed publisher
    b>RAB3A-interacting molecule (RIM) proteins are important regulators of transmitter release from active zones...
  13. Lenselink A, Rotaru D, Li K, Van Nierop P, Rao Ruiz P, Loos M, et al. Strain Differences in Presynaptic Function: PROTEOMICS, ULTRASTRUCTURE, AND PHYSIOLOGY OF HIPPOCAMPAL SYNAPSES IN DBA/2J AND C57Bl/6J MICE. J Biol Chem. 2015;290:15635-45 pubmed publisher
    ..expression of several presynaptic markers involved in exocytosis, such as syntaxin (Stx1b), Ras-related proteins (Rab3a/c), and rabphilin (Rph3a), was reduced...
  14. Sidhu V, Huang B, Desai A, Kevala K, Kim H. Role of DHA in aging-related changes in mouse brain synaptic plasma membrane proteome. Neurobiol Aging. 2016;41:73-85 pubmed publisher
    ..subunit epsilon-2 precursor, AMPA2, AP2, VGluT1, munc18-1, dynamin-1, vesicle-associated membrane protein 2, rab3A, and EAAT1, most of which are involved in synaptic transmission...
  15. Ito T, Matsubara D, Tanaka I, Makiya K, Tanei Z, Kumagai Y, et al. Loss of YAP1 defines neuroendocrine differentiation of lung tumors. Cancer Sci. 2016;107:1527-1538 pubmed publisher
    ..YAP1-sh-mediated knockdown induced the neuroendocrine marker RAB3a, which suggested the possible involvement of YAP1 in the regulation of neuroendocrine differentiation...
  16. Cazares V, Njus M, Manly A, Saldate J, Subramani A, Ben Simon Y, et al. Dynamic Partitioning of Synaptic Vesicle Pools by the SNARE-Binding Protein Tomosyn. J Neurosci. 2016;36:11208-11222 pubmed
    ..interactions that may contribute to these actions identified Tomo1 interaction with the GTP-bound state of Rab3A, an SV GTPase involved in SV targeting and presynaptic membrane tethering...
  17. Piper Hanley K, Hearn T, Berry A, Carvell M, Patch A, Williams L, et al. In vitro expression of NGN3 identifies RAB3B as the predominant Ras-associated GTP-binding protein 3 family member in human islets. J Endocrinol. 2010;207:151-61 pubmed publisher
    ..insulin exocytosis in rodent β-cells and responsiveness to incretins are reliant on Rab family members, notably Rab3a and Rab27a, but not Rab3b...
  18. Latchoumane C, Jackson L, Sendi M, Tehrani K, Mortensen L, Stice S, et al. Chronic Electrical Stimulation Promotes the Excitability and Plasticity of ESC-derived Neurons following Glutamate-induced Inhibition In vitro. Sci Rep. 2018;8:10957 pubmed publisher
    ..NMDA) receptor subunit (NR2A), brain-derived neurotrophic factor (BDNF) and Ras-related protein (RAB3A). In contrast, the simultaneous administration of LFS/DCS down-regulated BDNF and RAB3A expression...
  19. Eckschlager T, Adam V, Hrabeta J, Figova K, Kizek R. Metallothioneins and cancer. Curr Protein Pept Sci. 2009;10:360-75 pubmed
    ..MTs are also involved with several important proteins e.g. p53, NF-kappaB, PKCl, and GTPase Rab3A. New biological roles for these proteins have been identified including those needed in the carcinogenic process...
  20. Nishioka H, Tooi N, Isobe T, Nakatsuji N, Aiba K. BMS-708163 and Nilotinib restore synaptic dysfunction in human embryonic stem cell-derived Alzheimer's disease models. Sci Rep. 2016;6:33427 pubmed publisher
    ..In this study, we found that our AD models showed reduced levels of RAB3A and SV2B proteins in the pre-synapses, which is a possible cause of electrophysiological abnormalities...
  21. Spencer B, Desplats P, Overk C, Valera Martin E, Rissman R, Wu C, et al. Reducing Endogenous α-Synuclein Mitigates the Degeneration of Selective Neuronal Populations in an Alzheimer's Disease Transgenic Mouse Model. J Neurosci. 2016;36:7971-84 pubmed publisher
    ..prevented the degeneration of cholinergic neurons, ameliorated corresponding deficits, and recovered the levels of Rab3a and Rab5 proteins involved in intracellular transport and sorting of nerve growth factor and brain-derived ..
  22. Hong Y, Zhao T, Li X, Li S. Mutant Huntingtin Impairs BDNF Release from Astrocytes by Disrupting Conversion of Rab3a-GTP into Rab3a-GDP. J Neurosci. 2016;36:8790-801 pubmed publisher
    ..Our results demonstrate that mHtt associates with Rab3a, a small GTPase localized on membranes of dense-core vesicles, and prevents GTP-Rab3a from binding to Rab3-GAP1, ..
  23. de Siqueira K, de Lima F, Lima F, Taki M, da Cunha C, de Lima Reis S, et al. miR-124a expression contributes to the monophasic pattern of insulin secretion in islets from pregnant rats submitted to a low-protein diet. Eur J Nutr. 2018;57:1471-1483 pubmed publisher
    ..miR-124a expression was reduced by a low-protein diet. SNAP-25 mRNA, protein expression, and Rab3A protein content were lower in the LPP rats than in CP rats. Syntaxin 1A and Kir6...
  24. Osimo E, Beck K, Reis Marques T, Howes O. Synaptic loss in schizophrenia: a meta-analysis and systematic review of synaptic protein and mRNA measures. Mol Psychiatry. 2019;24:549-561 pubmed publisher
    ..There were insufficient studies for meta-analysis of complexins, synapsins, rab3A and synaptotagmin and mRNA measures...
  25. Tang X, Xie C, Wang Y, Wang X. Localization of Rab3A-binding site on C2A domain of synaptotagmin I to reveal its regulatory mechanism. Int J Biol Macromol. 2017;96:736-742 pubmed publisher
    ..Our previous study demonstrated that Rab3A, a small GTP-binding protein, is a new interacting partner of Syt I and could bind to both of the C2 domains; the ..
  26. Raiborg C, Stenmark H. Plasma membrane repairs by small GTPase Rab3a. J Cell Biol. 2016;213:613-5 pubmed publisher
    ..Now, Encarnação et al. (2016. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201511093) demonstrate that the lysosomal GTPase Rab3a and its effectors orchestrate lysosome positioning and plasma membrane repair.
  27. Wang H, Cui Q, Zhang T, Wang Z, Ouyang Y, Shen W, et al. Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation. Histochem Cell Biol. 2016;145:647-57 pubmed publisher
    ..In this study, we investigated the distribution and functions of three members of the Rab family, Rab3A, Rab27A, and Rab35, in mouse oocyte meiotic maturation and activation...
  28. Dai Z, Tang X, Chen J, Tang X, Wang X. Rab3A Inhibition of Ca2+ -Dependent Dopamine Release From PC12 Cells Involves Interaction With Synaptotagmin I. J Cell Biochem. 2017;118:3696-3705 pubmed publisher
    ..Here, we studied the effects of Rab3A and synaptotagmin I (Syt I) on dopamine release using PC12 cells as a model system...
  29. Burton J, Roberts D, Montaldi M, Novick P, De Camilli P. A mammalian guanine-nucleotide-releasing protein enhances function of yeast secretory protein Sec4. Nature. 1993;361:464-7 pubmed
    ..The Mss4 protein also stimulates GDP release from Ypt1 and from the mammalian protein Rab3a, but not from Ras2. Mss4 shows sequence similarity to Dss4, a yeast protein with similar biochemical properties.
  30. Wang T, Ma Z, Wang C, Liu C, Yan D, Deng Y, et al. Manganese-induced alpha-synuclein overexpression impairs synaptic vesicle fusion by disrupting the Rab3 cycle in primary cultured neurons. Toxicol Lett. 2018;285:34-42 pubmed publisher
    ..were exposed to Mn (100 μM) for 0, 6, 12, 24 h, [Ca2+]i, alpha-synuclein and Rab3A-GTP protein expression increased gradually...
  31. Liu S, Sahid M, Takemasa E, Maeyama K, Mogi M. Zoledronate modulates intracellular vesicle trafficking in mast cells via disturbing the interaction of myosinVa/Rab3a and sytaxin4/VAMP7. Biochem Pharmacol. 2018;151:18-25 pubmed publisher
    ..monitoring and biochemical results suggested that zoledronate modulates intracellular formation of the myosinVa/Rab3a complex and syntaxin4/VAMP7 complex, which are critical in vesicle motility, and therefore disturbs exocytosis via ..
  32. Li Y, Abdourahman A, Tamm J, Pehrson A, Sánchez C, Gulinello M. Reversal of age-associated cognitive deficits is accompanied by increased plasticity-related gene expression after chronic antidepressant administration in middle-aged mice. Pharmacol Biochem Behav. 2015;135:70-82 pubmed publisher
    ..g., Nfkb1, Fos, Fmr1, Camk2a, Arc, Shank1, Nlgn2, and Rab3a). Neither drug reversed the age-associated decrease in stem cell proliferation...
  33. Lee H, Min K, Yi J, Shi H, Chang W, Jackson L, et al. A Phosphoproteomic Screen Identifies a Guanine Nucleotide Exchange Factor for Rab3A Protein as a Mitogen-activated Protein (MAP) Kinase Phosphatase-5-regulated MAP Kinase Target in Interleukin 6 (IL-6) Secretion and Myogenesis. J Biol Chem. 2017;292:3581-3590 pubmed publisher
    ..We discovered a novel MKP-5-regulated MAPK substrate called guanine nucleotide exchange factor for Rab3A (GRAB) that was hyperphosphorylated on a phospho-MAPK motif in skeletal muscle of MKP-5-deficient mice...
  34. Zhou J, Liu Z, Yu J, Han X, Fan S, Shao W, et al. Quantitative Proteomic Analysis Reveals Molecular Adaptations in the Hippocampal Synaptic Active Zone of Chronic Mild Stress-Unsusceptible Rats. Int J Neuropsychopharmacol. 2015;19: pubmed publisher
    ..Moreover, through STRING and immunoblotting analysis, membrane-associated GTP-bound Rab3a and Munc18-1 appear to coregulate syntaxin-1/SNAP25/VAMP2 assembly at the hippocampal presynaptic active zone of ..
  35. Encarnação M, Espada L, Escrevente C, Mateus D, Ramalho J, Michelet X, et al. A Rab3a-dependent complex essential for lysosome positioning and plasma membrane repair. J Cell Biol. 2016;213:631-40 pubmed publisher
    ..b>Rab3a, which partially localizes to peripheral lysosomes, was one of the most robust hits...
  36. Wang C, Zhao D, Shah S, Yang W, Li C, Yang L. Proteome Analysis of Potential Synaptic Vesicle Cycle Biomarkers in the Cerebrospinal Fluid of Patients with Sporadic Creutzfeldt-Jakob Disease. Mol Neurobiol. 2017;54:5177-5191 pubmed publisher
    ..Six key proteins, such as AP2A1, SYT1, SNAP25, STXBP1, CLTB, and Rab3a, showed the same trend by western blot as detected by iTRAQ...
  37. Erickson J, Varoqui H, Schafer M, Modi W, Diebler M, Weihe E, et al. Functional identification of a vesicular acetylcholine transporter and its expression from a "cholinergic" gene locus. J Biol Chem. 1994;269:21929-32 pubmed
  38. Jung Y, Lee T, Lee J, Kim J, Park J. Phosphatidic acid is important to the translocation of Rab3A from the cytosol to phospholipid membranes. Neuroreport. 1999;10:2859-63 pubmed
    b>RAB3A, a Ras-related GTP-binding protein, is found in a rat neuronal cytosol in the form of complex with RabGDI or located on the synaptic vesicles and it cycles between the cytosol and the membranes...
  39. Haynes L, Evans G, Morgan A, Burgoyne R. A direct inhibitory role for the Rab3-specific effector, Noc2, in Ca2+-regulated exocytosis in neuroendocrine cells. J Biol Chem. 2001;276:9726-32 pubmed
    ..b>Rab3A is a neural/neuroendocrine-specific member of the Rab family involved in Ca(2+) -regulated exocytosis, where it ..
  40. Zhang Y, Luan Z, Liu A, Hu G. The scaffolding protein CASK mediates the interaction between rabphilin3a and beta-neurexins. FEBS Lett. 2001;497:99-102 pubmed
    ..It was proposed that presynaptic vesicles might be guided to the vicinity of points of exocytosis defined by beta-neurexins via the interaction between rabphilin3a-CASK-beta-neurexins. ..
  41. Tomes C, Michaut M, De Blas G, Visconti P, Matti U, Mayorga L. SNARE complex assembly is required for human sperm acrosome reaction. Dev Biol. 2002;243:326-38 pubmed
    ..permeabilization protocol developed in our laboratory, we have previously demonstrated requirements for Rab3A, NSF, and synaptotagmin VI in the human sperm acrosome reaction...
  42. Rastaldi M, Armelloni S, Berra S, Li M, Pesaresi M, Poczewski H, et al. Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a. Am J Pathol. 2003;163:889-99 pubmed
    ..In neurons, the small guanosine triphosphatase Rab3A and its effector rabphilin-3A form a complex required for the correct docking of synaptic vesicles to their target ..
  43. Santolini I, Celli R, Cannella M, Imbriglio T, Guiducci M, Parisi P, et al. Alterations in the ?2 ? ligand, thrombospondin-1, in a rat model of spontaneous absence epilepsy and in patients with idiopathic/genetic generalized epilepsies. Epilepsia. 2017;58:1993-2001 pubmed publisher
    ..measured the transcripts of thrombospondin-1 and ?2 ? subunit, and protein levels of ?2 ?, Rab3A, and the vesicular glutamate transporter, VGLUT1, in the somatosensory cortex and ventrobasal thalamus of ..
  44. Trask B, Fertitta A, Christensen M, Youngblom J, Bergmann A, Copeland A, et al. Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 genes or DNA markers. Genomics. 1993;15:133-45 pubmed
    ..FISH mapping of these cosmids sublocalizes a total of 70 genes and DNA markers on chromosome 19, revises the previously published map assignments of 2 genes, and narrows the location of over 20 markers. ..
  45. Burton J, Slepnev V, De Camilli P. An evolutionarily conserved domain in a subfamily of Rabs is crucial for the interaction with the guanyl nucleotide exchange factor Mss4. J Biol Chem. 1997;272:3663-8 pubmed
    ..the domain(s) of the GTPase that is important for this interaction, protein chimeras were constructed between Rab3a, which binds Mss4, and Rab5a, which does not bind Mss4...
  46. Fukuda M, Kanno E, Saegusa C, Ogata Y, Kuroda T. Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J Biol Chem. 2002;277:39673-8 pubmed
    ..Previously, we showed that the N-terminal Slp homology domain of Slp4-a interacts with the GTP-bound form of Rab3A, Rab8, and Rab27A both in vitro and in intact cells (Kuroda, T. S., Fukuda, M., Ariga, H., and Mikoshiba, K...
  47. Kapfhamer D, Valladares O, Sun Y, Nolan P, Rux J, Arnold S, et al. Mutations in Rab3a alter circadian period and homeostatic response to sleep loss in the mouse. Nat Genet. 2002;32:290-5 pubmed
    b>Rab3a is the most abundant Rab (ras-associated binding) protein in the brain and has a regulatory role in synaptic vesicle trafficking...
  48. Sun Y, Zhang F, Gao J, Gao X, Guo T, Shi Y, et al. Variants in the RAB3A gene are not associated with mental retardation in the Chinese population. Neurosci Lett. 2006;401:114-8 pubmed
    ..b>Rab3A, a member of the Rab small G protein family, is a key molecule in modulating basal neurotransmission and ..
  49. Kwan E, Xie L, Sheu L, Ohtsuka T, Gaisano H. Interaction between Munc13-1 and RIM is critical for glucagon-like peptide-1 mediated rescue of exocytotic defects in Munc13-1 deficient pancreatic beta-cells. Diabetes. 2007;56:2579-88 pubmed
    ..via cAMP actions on exchange protein directly activated by cAMP (Epac2) and protein kinase A (PKA)-activated Rab3A-interacting molecule 2 (Rim2)...
  50. Bustos M, Lucchesi O, Ruete M, Mayorga L, Tomes C. Rab27 and Rab3 sequentially regulate human sperm dense-core granule exocytosis. Proc Natl Acad Sci U S A. 2012;109:E2057-66 pubmed publisher
    ..The levels of GTP-bound Rab27 increased on initiation of exocytosis, as did the proportion of GTP-bound Rab3A. We have developed a fluorescence microscopy-based method for detecting endogenous Rab3A-GTP and Rab27-GTP in the ..
  51. Chen R, Wislet Gendebien S, Samuel F, Visanji N, Zhang G, Marsilio D, et al. ?-Synuclein membrane association is regulated by the Rab3a recycling machinery and presynaptic activity. J Biol Chem. 2013;288:7438-49 pubmed publisher
    ..involved in ?-synuclein membrane interactions and showed that antibodies directed to the Ras-related GTPase Rab3a and its chaperone RabGDI abrogated ?-synuclein membrane binding...
  52. Kim J, Lee S, Park C, Park S, Yin J, Kim J, et al. Rab3a promotes brain tumor initiation and progression. Mol Biol Rep. 2014;41:5903-11 pubmed publisher
    ..In particular, Rab3a which is one of four Rab3 proteins (a, b, c, and d isoforms) is associated with synaptic vesicle trafficking in ..
  53. Pelletán L, Suhaiman L, Vaquer C, Bustos M, De Blas G, Vitale N, et al. ADP ribosylation factor 6 (ARF6) promotes acrosomal exocytosis by modulating lipid turnover and Rab3A activation. J Biol Chem. 2015;290:9823-41 pubmed publisher
    ..We show that active ARF6 increases the exchange of GDP for GTP on Rab3A, a prerequisite for secretion...
  54. Bereczki E, Francis P, Howlett D, Pereira J, Höglund K, Bogstedt A, et al. Synaptic proteins predict cognitive decline in Alzheimer's disease and Lewy body dementia. Alzheimers Dement. 2016;12:1149-1158 pubmed publisher
    ..was to compare the levels of three synaptic proteins involved in different steps of the synaptic transmission: Rab3A, SNAP25, and neurogranin, in three common forms of dementia: Alzheimer's disease (AD), dementia with Lewy bodies (..
  55. Quevedo M, Lucchesi O, Bustos M, Pocognoni C, De la Iglesia P, Tomes C. The Rab3A-22A Chimera Prevents Sperm Exocytosis by Stabilizing Open Fusion Pores. J Biol Chem. 2016;291:23101-23111 pubmed
    ..It is believed that immediately afterward, fusion pores dilate spontaneously. Rab3A is an essential component of human sperm exocytotic machinery...
  56. Khosravi Far R, Lutz R, Cox A, Conroy L, Bourne J, Sinensky M, et al. Isoprenoid modification of rab proteins terminating in CC or CXC motifs. Proc Natl Acad Sci U S A. 1991;88:6264-8 pubmed
    ..rab1b and rab3a proteins expressed in insect cells incorporated a product of [3H]mevalonate, and gas chromatography analysis of ..
  57. Weber E, Jilling T, Kirk K. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J Biol Chem. 1996;271:6963-71 pubmed
    b>Rab3A and Rab3B are highly homologous monomeric GTPases that are putative regulators of exocytosis in those tissues in which they are expressed...
  58. Piiper A, Leser J, Lutz M, Beil M, Zeuzem S. Subcellular distribution and function of Rab3A-D in pancreatic acinar AR42J cells. Biochem Biophys Res Commun. 2001;287:746-51 pubmed
    ..recently shown by Northern blot analysis that pancreatic acinar-like AR42J cells express all four Rab3 isoforms (Rab3A-D)...
  59. Kuroda T, Fukuda M, Ariga H, Mikoshiba K. Synaptotagmin-like protein 5: a novel Rab27A effector with C-terminal tandem C2 domains. Biochem Biophys Res Commun. 2002;293:899-906 pubmed
    ..family members, the SHD of Slp5 preferentially interacted with the GTP-bound form of Rab27A and marginally with Rab3A and Rab6A, both in vitro and in intact cells, but not with other Rabs tested (Rab1, Rab2, Rab4A, Rab5A, Rab7, Rab8,..
  60. Gasnier B. The SLC32 transporter, a key protein for the synaptic release of inhibitory amino acids. Pflugers Arch. 2004;447:756-9 pubmed
  61. Takao Rikitsu E, Mochida S, Inoue E, Deguchi Tawarada M, Inoue M, Ohtsuka T, et al. Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release. J Cell Biol. 2004;164:301-11 pubmed
    ..These results indicate that CAST serves as a key component of the CAZ structure and is involved in neurotransmitter release by binding these CAZ proteins. ..
  62. D Adamo P, Bacchelli E, Blasi F, Lipp H, Toniolo D, Maestrini E. DNA variants in the human RAB3A gene are not associated with autism. Genes Brain Behav. 2004;3:123-4 pubmed
    Mutation screening of the RAB3A gene in 47 individuals with autism provided no evidence that DNA variants in this gene are associated with autism...
  63. Dalfo E, Barrachina M, Rosa J, Ambrosio S, Ferrer I. Abnormal alpha-synuclein interactions with rab3a and rabphilin in diffuse Lewy body disease. Neurobiol Dis. 2004;16:92-7 pubmed
    The present study examines alpha-synuclein interactions with rab3a and rabphilin by antibody arrays, immunoprecipitation and pull-down methods in the entorhinal cortex of control cases and in diffuse Lewy body disease (LBD) cases...
  64. Dalfo E, Ferrer I. Alpha-synuclein binding to rab3a in multiple system atrophy. Neurosci Lett. 2005;380:170-5 pubmed
    ..Combined alpha-synuclein and rab3a immunoprecipitation assays have shown alpha-synuclein/rab3a binding in the cerebellum and pons (in which GCIs were ..
  65. Quick M. The role of SNARE proteins in trafficking and function of neurotransmitter transporters. Handb Exp Pharmacol. 2006;:181-96 pubmed
    ..In this chapter, the functional effects of syntaxin-transporter interactions are reviewed, and how such interactions may regulate neuronal signaling are considered. ..
  66. Lopez C, Belmonte S, De Blas G, Mayorga L. Membrane-permeant Rab3A triggers acrosomal exocytosis in living human sperm. FASEB J. 2007;21:4121-30 pubmed
    ..However, our results indicate that GST and Rab3A, when fused with an arginine-rich peptide, were able to translocate into sperm...
  67. Figueiredo A, Wasmeier C, Tarafder A, Ramalho J, Baron R, Seabra M. Rab3GEP is the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes. J Biol Chem. 2008;283:23209-16 pubmed publisher
    ..Here, we studied the activation of Rab27a in melanocytes. We identify Rab3GEP, previously isolated as a GEF for Rab3a, as the non-redundant Rab27a GEF...
  68. Lodhi S, Farmer R, Singh A, Jaiswal Y, Wadhwa G. 3D structure generation, virtual screening and docking of human Ras-associated binding (Rab3A) protein involved in tumourigenesis. Mol Biol Rep. 2014;41:3951-9 pubmed publisher
    b>Rab3A is expressed predominantly in brain and synaptic vesicles. Rab3A is involved specifically in tethering and docking of synaptic vesicles prior to fusion which is a critical step in regulated release of neurotransmitters...
  69. Rousseau Merck M, Zahraoui A, Bernheim A, Touchot N, Miglierina R, Tavitian A, et al. Chromosome mapping of the human ras-related rab3A gene to 19p13.2. Genomics. 1989;5:694-8 pubmed
    ..We used the human rab3A cDNA to map the corresponding gene on human chromosomes by chromosome sorting and in situ hybridization...
  70. Brondyk W, McKiernan C, Fortner K, Stabila P, Holz R, Macara I. Interaction cloning of Rabin3, a novel protein that associates with the Ras-like GTPase Rab3A. Mol Cell Biol. 1995;15:1137-43 pubmed
    b>Rab3A is a small, Ras-like GTPase expressed in neuroendocrine cells, in which it is associated with secretory vesicle membranes and regulates exocytosis...
  71. McKiernan C, Brondyk W, Macara I. The Rab3A GTPase interacts with multiple factors through the same effector domain. Mutational analysis of cross-linking of Rab3A to a putative target protein. J Biol Chem. 1993;268:24449-52 pubmed
    b>Rab3A/smg25A is a small Ras-like guanine nucleotide binding protein implicated in the control of regulated secretion from cells. Rab3A is approximately 30% cytosolic and 70% associated with the membranes of secretory vesicles...
  72. Johannes L, Perez F, Laran Chich M, Henry J, Darchen F. Characterization of the interaction of the monomeric GTP-binding protein Rab3a with geranylgeranyl transferase II. Eur J Biochem. 1996;239:362-8 pubmed
    The monomeric GTP-binding protein Rab3a controls exocytosis in neuroendocrine and neuronal cells...
  73. Martincic I, Peralta M, Ngsee J. Isolation and characterization of a dual prenylated Rab and VAMP2 receptor. J Biol Chem. 1997;272:26991-8 pubmed
    ..Using the yeast two-hybrid screen, we have isolated a rat clone that interacts with Rab3A as well as with Rab1. The gene encodes a 20...
  74. Clabecq A, Henry J, Darchen F. Biochemical characterization of Rab3-GTPase-activating protein reveals a mechanism similar to that of Ras-GAP. J Biol Chem. 2000;275:31786-91 pubmed
    ..We have characterized the activity of recombinant Rab3-GAP on Rab3A in solution. The K(m) and K(d) values (75 microm) indicate a low affinity of Rab3-GAP for its substrate...
  75. Tabellini G, Baldini G, Bortul R, Bareggi R, Narducci P, Martelli A. Localization of the small monomeric GTPases Rab3D and Rab3A in the AtT-20 rat pituitary cell line. Eur J Histochem. 2001;45:347-56 pubmed
    We investigated the cellular localization of the small GTPases Rab3D and Rab3A in AtT-20 cells treated with the drug Brefeldin A...
  76. Pereira Leal J, Strom M, Godfrey R, Seabra M. Structural determinants of Rab and Rab Escort Protein interaction: Rab family motifs define a conserved binding surface. Biochem Biophys Res Commun. 2003;301:92-7 pubmed
    ..We introduced point mutations in Rab3a as a model Rab and assessed the ability of the mutants to interact with REP using the yeast two-hybrid system and ..
  77. Belmonte S, López C, Roggero C, De Blas G, Tomes C, Mayorga L. Cholesterol content regulates acrosomal exocytosis by enhancing Rab3A plasma membrane association. Dev Biol. 2005;285:393-408 pubmed
    ..Functional data indicate that one target for the cholesterol effect is Rab3A. The sterol content does not affect the Rab3A activation-deactivation cycle but regulates its membrane anchoring...
  78. Branham M, Bustos M, De Blas G, Rehmann H, Zarelli V, Trevino C, et al. Epac activates the small G proteins Rap1 and Rab3A to achieve exocytosis. J Biol Chem. 2009;284:24825-39 pubmed publisher
    ..In addition, our studies identify a novel connection between cAMP and Rab3A, a secretory granule-associated protein, revealing that the latter functions downstream of soluble adenylyl ..
  79. Szodorai A, Kuan Y, Hunzelmann S, Engel U, Sakane A, Sasaki T, et al. APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle. J Neurosci. 2009;29:14534-44 pubmed publisher
    ..that APP is a cargo of a vesicle containing the kinesin heavy chain isoform kinesin-1C, the small GTPase Rab3A, and a specific subset of presynaptic protein components...
  80. Bello O, Cappa A, de Paola M, Zanetti M, Fukuda M, Fissore R, et al. Rab3A, a possible marker of cortical granules, participates in cortical granule exocytosis in mouse eggs. Exp Cell Res. 2016;347:42-51 pubmed publisher
    ..b>Rab3A, a member of the small GTP-binding protein superfamily, has been implicated in calcium-dependent exocytosis and is ..
  81. Lis P, Burel S, Steger M, Mann M, Brown F, Diez F, et al. Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson's disease kinase. Biochem J. 2018;475:1-22 pubmed publisher
    ..and monoclonal phospho-specific antibodies raised against nine different LRRK2-phosphorylated Rab proteins (Rab3A/3B/3C/3D, Rab5A/5B/5C, Rab8A/8B, Rab10, Rab12, Rab29[T71], Rab29[S72], Rab35 and Rab43)...
  82. Vielh E, Touchot N, Zahraoui A, Tavitian A. Nucleotide sequence of a rat cDNA: rab1B, encoding a rab1-YPT related protein. Nucleic Acids Res. 1989;17:1770 pubmed
  83. Zahraoui A, Touchot N, Chardin P, Tavitian A. The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion. J Biol Chem. 1989;264:12394-401 pubmed
    Seven cDNA clones corresponding to the rab1, rab2, rab3A, rab3B, rab4, rab5, and rab6 genes were isolated from a human pheochromocytoma cDNA library...
  84. Farnsworth C, Seabra M, Ericsson L, Gelb M, Glomset J. Rab geranylgeranyl transferase catalyzes the geranylgeranylation of adjacent cysteines in the small GTPases Rab1A, Rab3A, and Rab5A. Proc Natl Acad Sci U S A. 1994;91:11963-7 pubmed
    ..Rab escort protein, and [1-3H]geranylgeranyl pyrophosphate with recombinant wild-type Rab1A (-XXCC), Rab3A (-XCXC), or Rab5A (-CCXX) and treated each labeled protein with trypsin...
  85. Cremers F, Armstrong S, Seabra M, Brown M, Goldstein J. REP-2, a Rab escort protein encoded by the choroideremia-like gene. J Biol Chem. 1994;269:2111-7 pubmed
    ..However, REP-2 was only 25% as active as REP-1 in supporting GG attachment to Rab3A and Rab3D...
  86. Ostermeier C, Brunger A. Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A. Cell. 1999;96:363-74 pubmed
    The small G protein Rab3A plays an important role in the regulation of neurotransmitter release. The crystal structure of activated Rab3A/GTP/Mg2+ bound to the effector domain of rabphilin-3A was solved to 2.6 A resolution...
  87. Sullivan M, Olsen A, Houslay M. Genomic organisation of the human cyclic AMP-specific phosphodiesterase PDE4C gene and its chromosomal localisation to 19p13.1, between RAB3A and JUND. Cell Signal. 1999;11:735-42 pubmed
    ..genes for the growth regulatory transcription factor, JUND, and the mini guanine nucleotide regulatory protein, RAB3A. The RAB3A gene was shown to consist of 5 exons spanning 7...
  88. Matsumoto M, Miki T, Shibasaki T, Kawaguchi M, Shinozaki H, Nio J, et al. Noc2 is essential in normal regulation of exocytosis in endocrine and exocrine cells. Proc Natl Acad Sci U S A. 2004;101:8313-8 pubmed
    ..Thus, Noc2, a critical effector of Rab3, is essential in normal regulation of exocytosis in both endocrine and exocrine cells. ..
  89. Zografou S, Basagiannis D, Papafotika A, Shirakawa R, Horiuchi H, Auerbach D, et al. A complete Rab screening reveals novel insights in Weibel-Palade body exocytosis. J Cell Sci. 2012;125:4780-90 pubmed publisher
    ..These data indicate that WPB secretion requires the coordinated function of a specific group of Rabs and that, among them, Rab27a and Rab15, as well as their effector Munc13-4, cooperate to drive exocytosis. ..
  90. Lindsay A, Jollivet F, Horgan C, Khan A, Raposo G, McCaffrey M, et al. Identification and characterization of multiple novel Rab-myosin Va interactions. Mol Biol Cell. 2013;24:3420-34 pubmed publisher
    ..We also present evidence that myosin Va is necessary for maintaining a peripheral distribution of Rab11- and Rab14-positive endosomes. ..
  91. Gerondopoulos A, Bastos R, Yoshimura S, Anderson R, Carpanini S, Aligianis I, et al. Rab18 and a Rab18 GEF complex are required for normal ER structure. J Cell Biol. 2014;205:707-20 pubmed publisher
    ..Micro syndrome is therefore a disease characterized by direct loss of Rab18 function or loss of Rab18 activation at the ER by its GEF Rab3GAP. ..
  92. Steger M, Diez F, Dhekne H, Lis P, Nirujogi R, Karayel Ö, et al. Systematic proteomic analysis of LRRK2-mediated Rab GTPase phosphorylation establishes a connection to ciliogenesis. elife. 2017;6: pubmed publisher
    ..2016) (PMID: 26824392). Here, we systematically analyzed the Rab protein family and found 14 of them (Rab3A/B/C/D, Rab5A/B/C, Rab8A/B, Rab10, Rab12, Rab29, Rab35 and Rab43) to be specifically phosphorylated by LRRK2, with ..