Rab27a

Summary

Gene Symbol: Rab27a
Description: RAB27A, member RAS oncogene family
Alias: GS2, HsT18676, RAB27, RAM, ras-related protein Rab-27A, GTP-binding protein Ram, mutant Ras-related protein Rab-27A, rab-27
Species: human
Products:     Rab27a

Top Publications

  1. Chen D, Guo J, Miki T, Tachibana M, Gahl W. Molecular cloning and characterization of rab27a and rab27b, novel human rab proteins shared by melanocytes and platelets. Biochem Mol Med. 1997;60:27-37 pubmed
    ..We isolated the complete cDNAs of two rab isoforms, rab27a and rab27b, from human melanoma cells and melanocytes, Rab27a is the human homolog of a rat megakaryocyte rab ..
  2. Sanal O, Ersoy F, Tezcan I, Metin A, Yel L, Menasche G, et al. Griscelli disease: genotype-phenotype correlation in an array of clinical heterogeneity. J Clin Immunol. 2002;22:237-43 pubmed
    ..Eight patients who showed RAB27A mutations presented with HS...
  3. Wang H, Zhao Y, Zhang C, Li M, Jiang C, Li Y. Rab27a was identified as a prognostic biomaker by mRNA profiling, correlated with malignant progression and subtype preference in gliomas. PLoS ONE. 2014;9:e89782 pubmed publisher
    b>Rab27a belongs to the Rab small GTPase superfamily. The protein is membrane-bound and may be involved in protein transport and small GTPase-mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2...
  4. Shirakawa R, Higashi T, Tabuchi A, Yoshioka A, Nishioka H, Fukuda M, et al. Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets. J Biol Chem. 2004;279:10730-7 pubmed
    ..Here, we show that incubation of permeabilized platelets with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited the secretion, whereas inactive mutant Rab27A-T23N and other GTPases ..
  5. Menasche G, Ménager M, Lefebvre J, Deutsch E, Athman R, Lambert N, et al. A newly identified isoform of Slp2a associates with Rab27a in cytotoxic T cells and participates to cytotoxic granule secretion. Blood. 2008;112:5052-62 pubmed publisher
    ..This process involves polarization of the CTL's secretory machinery and cytotoxic granules. The small GTPase Rab27a and the hMunc13-4 protein have been shown to be required for both granule maturation and granule docking and ..
  6. Wang J, Wang F, Zhang Q, Shen Z, Shao Z. Enhanced expression of Rab27A gene by breast cancer cells promoting invasiveness and the metastasis potential by secretion of insulin-like growth factor-II. Mol Cancer Res. 2008;6:372-82 pubmed publisher
    ..to the functions of transporting melanosome in melanocytes and releasing contents of lytic granules in CTLs, Rab27A was recently shown to be involved in exocytosis of insulin and chromaffin granules in endocrine cells; it was also ..
  7. Chiaverini C, Beuret L, Flori E, Busca R, Abbe P, Bille K, et al. Microphthalmia-associated transcription factor regulates RAB27A gene expression and controls melanosome transport. J Biol Chem. 2008;283:12635-42 pubmed publisher
    ..factor (MITF) silencing induces melanosome gathering around the nucleus and causes the relocalization of Rab27A, Slac2a-Mlph, and Myo5a that control the transport of melanosomes on the actin network...
  8. Holt O, Kanno E, Bossi G, Booth S, Daniele T, Santoro A, et al. Slp1 and Slp2-a localize to the plasma membrane of CTL and contribute to secretion from the immunological synapse. Traffic. 2008;9:446-57 pubmed publisher
    b>Rab27a is required for polarized secretion of lysosomes from cytotoxic T lymphocytes (CTLs) at the immunological synapse...
  9. Ménager M, Menasche G, Romao M, Knapnougel P, Ho C, Garfa M, et al. Secretory cytotoxic granule maturation and exocytosis require the effector protein hMunc13-4. Nat Immunol. 2007;8:257-67 pubmed
    ..b>Rab27a and hMunc13-4 are critical effectors of the exocytosis of cytotoxic granules...

More Information

Publications99

  1. Munafo D, Johnson J, Ellis B, Rutschmann S, Beutler B, Catz S. Rab27a is a key component of the secretory machinery of azurophilic granules in granulocytes. Biochem J. 2007;402:229-39 pubmed
    ..The secretory machinery utilized by neutrophils is poorly characterized. We show that the small GTPase Rab27a is an essential component of the secretory machinery of azurophilic granules in granulocytes...
  2. Neeft M, Wieffer M, de Jong A, Negroiu G, Metz C, van Loon A, et al. Munc13-4 is an effector of rab27a and controls secretion of lysosomes in hematopoietic cells. Mol Biol Cell. 2005;16:731-41 pubmed
    ..The disease is caused by the absence of functional rab27a, but how rab27a controls secretion of lytic granule contents remains elusive...
  3. Yokoyama K, Kaji H, He J, Tanaka C, Hazama R, Kamigaki T, et al. Rab27a negatively regulates phagocytosis by prolongation of the actin-coating stage around phagosomes. J Biol Chem. 2011;286:5375-82 pubmed publisher
    b>Rab27a, a Rab family small GTPase, is involved in the exocytosis of secretory granules in melanocytes and cytotoxic T-cells...
  4. Fraile Ramos A, Cepeda V, Elstak E, van der Sluijs P. Rab27a is required for human cytomegalovirus assembly. PLoS ONE. 2010;5:e15318 pubmed publisher
    ..The mechanisms underlying these processes are not understood. Here we studied the role of Rab27a, a regulator of lysosome-related organelle transport, in HCMV production...
  5. Passeron T, Bahadoran P, Bertolotto C, Chiaverini C, Busca R, Valony G, et al. Cyclic AMP promotes a peripheral distribution of melanosomes and stimulates melanophilin/Slac2-a and actin association. FASEB J. 2004;18:989-91 pubmed
    ..We demonstrate that cAMP stimulates the expression of Rab27a and rapidly increases the interaction of the melanophilin/Slac2-a with actin...
  6. Tolmachova T, Ramalho J, Anant J, Schultz R, Huxley C, Seabra M. Cloning, mapping and characterization of the human RAB27A gene. Gene. 1999;239:109-16 pubmed
    ..REP1 acts in the prenylation of Rab GTPases, regulators of intracellular protein trafficking. Rab27a is unique among Rabs in that it is selectively unprenylated in CHM cells, suggesting that the degenerative process ..
  7. Seabra M, Ho Y, Anant J. Deficient geranylgeranylation of Ram/Rab27 in choroideremia. J Biol Chem. 1995;270:24420-7 pubmed
    ..This protein was purified and identified as Ram (renamed Rab27), a previously cloned Rab of unknown function...
  8. Barral D, Ramalho J, Anders R, Hume A, Knapton H, Tolmachova T, et al. Functional redundancy of Rab27 proteins and the pathogenesis of Griscelli syndrome. J Clin Invest. 2002;110:247-57 pubmed
    ..This disease is caused by loss-of-function mutations in RAB27A, which encodes 1 of the 60 known Rab GTPases, critical regulators of vesicular transport...
  9. Haddad E, Wu X, Hammer J, Henkart P. Defective granule exocytosis in Rab27a-deficient lymphocytes from Ashen mice. J Cell Biol. 2001;152:835-42 pubmed
    Because mutations in Rab27a have been linked to immune defects in humans, we have examined cytotoxic lymphocyte function in ashen mice, which contain a splicing mutation in Rab27a...
  10. Bahadoran P, Aberdam E, Mantoux F, Busca R, Bille K, Yalman N, et al. Rab27a: A key to melanosome transport in human melanocytes. J Cell Biol. 2001;152:843-50 pubmed
    ..However, it was demonstrated recently that GS can also be caused by a mutation in the Rab27a gene. These observations prompted us to investigate the role of Rab27a in melanosome transport...
  11. Menasche G, Ho C, Sanal O, Feldmann J, Tezcan I, Ersoy F, et al. Griscelli syndrome restricted to hypopigmentation results from a melanophilin defect (GS3) or a MYO5A F-exon deletion (GS1). J Clin Invest. 2003;112:450-6 pubmed
    ..Myosin Va (MyoVa) cause GS1 and the dilute mutant in mice, whereas mutations in the gene encoding the small GTPase Rab27a are responsible for GS2 and the ashen mouse model...
  12. Strom M, Hume A, Tarafder A, Barkagianni E, Seabra M. A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport. J Biol Chem. 2002;277:25423-30 pubmed
    The Rab27a GTPase regulates diverse processes involving lysosome-related organelles, including melanosome motility in melanocytes, and lytic granule release in cytotoxic T lymphocytes...
  13. Yi Z, Yokota H, Torii S, Aoki T, Hosaka M, Zhao S, et al. The Rab27a/granuphilin complex regulates the exocytosis of insulin-containing dense-core granules. Mol Cell Biol. 2002;22:1858-67 pubmed
    ..by a yeast two-hybrid system revealed that an amino-terminal zinc-finger domain of granuphilin interacts with Rab27a. Granuphilin preferentially bound to the GTP form of Rab27a...
  14. Ostrowski M, Carmo N, Krumeich S, Fanget I, Raposo G, Savina A, et al. Rab27a and Rab27b control different steps of the exosome secretion pathway. Nat Cell Biol. 2010;12:19-30; sup pp 1-13 pubmed publisher
    ..Among these, Rab27a and Rab27b were found to function in MVE docking at the plasma membrane...
  15. Figueiredo A, Wasmeier C, Tarafder A, Ramalho J, Baron R, Seabra M. Rab3GEP is the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes. J Biol Chem. 2008;283:23209-16 pubmed publisher
    ..b>Rab27a controls motility and regulated exocytosis of secretory granules and related organelles...
  16. Menasche G, Pastural E, Feldmann J, Certain S, Ersoy F, Dupuis S, et al. Mutations in RAB27A cause Griscelli syndrome associated with haemophagocytic syndrome. Nat Genet. 2000;25:173-6 pubmed
    ..1-cM interval between D15S1003 and D15S962. We detected mutations in RAB27A, which lies within this interval, in 16 patients with GS...
  17. Kondo H, Shirakawa R, Higashi T, Kawato M, Fukuda M, Kita T, et al. Constitutive GDP/GTP exchange and secretion-dependent GTP hydrolysis activity for Rab27 in platelets. J Biol Chem. 2006;281:28657-65 pubmed
    We have previously demonstrated that Rab27 regulates dense granule secretion in platelets...
  18. Akavia U, Litvin O, Kim J, Sanchez Garcia F, Kotliar D, Causton H, et al. An integrated approach to uncover drivers of cancer. Cell. 2010;143:1005-17 pubmed publisher
    ..Two dependencies, TBC1D16 and RAB27A, confirmed empirically, suggest that abnormal regulation of protein trafficking contributes to proliferation in ..
  19. Yamaguchi Y, Hearing V. Physiological factors that regulate skin pigmentation. Biofactors. 2009;35:193-9 pubmed publisher
    ..Microtubules, dynein, kinesin, actin filaments, Rab27a, melanophilin, myosin Va, and Slp2-a are involved in melanosome transport...
  20. Kudo M, Kobayashi Nakamura K, Tsuji Naito K. Bifunctional effects of O-methylated flavones from Scutellaria baicalensis Georgi on melanocytes: Inhibition of melanin production and intracellular melanosome transport. PLoS ONE. 2017;12:e0171513 pubmed publisher
    ..Other actin-based melanosome transport-related molecules, i.e., Rab27A and myosin Va, were not affected by wogonin...
  21. Otręba M, Buszman E, Miliński M, Wrześniok D. [Hypomelanoses transmitted from generation to generation]. Postepy Hig Med Dosw (Online). 2014;68:1081-90 pubmed
    ..and are caused by different mutations of the following genes: TYR, P, TRP1, MATP, HPS, CHS, MYO5A, RAB27A, MLPH, ATP7A and PAH...
  22. Panigrahi I, Suthar R, Rawat A, Behera B. Seizure as the presenting manifestation in Griscelli syndrome type 2. Pediatr Neurol. 2015;52:535-8 pubmed publisher
    ..Griscelli syndrome type 2 is caused by mutations in the RAB27A gene and has predominant immunologic abnormalities...
  23. Mokhtar D, Hussein M, Hassan A. Melatonin Elicits Stimulatory Action on the Adrenal Gland of Soay Ram: Morphometrical, Immunohistochemical, and Ultrastructural Study. Microsc Microanal. 2017;:1-16 pubmed publisher
    ..Adrenal glands of 15 Soay ram were examined to detect the effect of melatonin treatment...
  24. Abd Elhafeez H, Mokhtar D, Hassan A. Effect of Melatonin on Telocytes in the Seminal Vesicle of the Soay Ram: An Immunohistochemical, Ultrastructural and Morphometrical Study. Cells Tissues Organs. 2017;203:29-54 pubmed publisher
    ..The current study aimed to investigate TCs in seminal vesicles of the Soay ram responding to melatonin treatment during the nonbreeding season by conventional immunohistochemical stains, and to ..
  25. Chinen J, Notarangelo L, Shearer W. Advances in clinical immunology in 2015. J Allergy Clin Immunol. 2016;138:1531-1540 pubmed publisher
    ..elongation (RTEL1) mutation causing isolated natural killer cell deficiency and mutations in ras-associated RAB (RAB27) resulting in immunodeficiency without albinism...
  26. Westbroek W, Klar A, Cullinane A, Ziegler S, Hurvitz H, Ganem A, et al. Cellular and clinical report of new Griscelli syndrome type III cases. Pigment Cell Melanoma Res. 2012;25:47-56 pubmed publisher
    The RAB27A/Melanophilin/Myosin-5a tripartite protein complex is required for capturing mature melanosomes in the peripheral actin network of melanocytes for subsequent transfer to keratinocytes...
  27. Luna C, Serrano E, Domingo J, Casao A, Pérez Pé R, Cebrian Perez J, et al. Expression, cellular localization, and involvement of the pentose phosphate pathway enzymes in the regulation of ram sperm capacitation. Theriogenology. 2016;86:704-14 pubmed publisher
    ..These findings can be of high-biological importance to improve our knowledge of the biochemical mechanisms involved in the acquisition of mammalian sperm functional competence and, ultimately, fertility. ..
  28. Meeths M, Bryceson Y, Rudd E, Zheng C, Wood S, Ramme K, et al. Clinical presentation of Griscelli syndrome type 2 and spectrum of RAB27A mutations. Pediatr Blood Cancer. 2010;54:563-72 pubmed publisher
    Griscelli syndrome type 2 (GS2) is an autosomal-recessive immunodeficiency caused by mutations in RAB27A, clinically characterized by partial albinism and haemophagocytic lymphohistocytosis (HLH)...
  29. Masoudi R, Sharafi M, Zare Shahneh A, Towhidi A, Kohram H, Zhandi M, et al. Effect of dietary fish oil supplementation on ram semen freeze ability and fertility using soybean lecithin- and egg yolk-based extenders. Theriogenology. 2016;86:1583-1588 pubmed publisher
    b>Ram semen cryopreservation is not efficient for artificial insemination in commercial herds...
  30. Vahdat L, Layman R, Yardley D, Gradishar W, Salkeni M, Joy A, et al. Randomized Phase II Study of Ramucirumab or Icrucumab in Combination with Capecitabine in Patients with Previously Treated Locally Advanced or Metastatic Breast Cancer. Oncologist. 2017;22:245-254 pubmed publisher
    Icrucumab (ICR) and ramucirumab (RAM) bind vascular endothelial growth factor (VEGF) receptors 1 and 2 (VEGFR-1 and -2), respectively...
  31. De Luca A, Sidumo Z, Zanelli G, Magid N, Luhanga R, Brambilla D, et al. Accumulation of HIV-1 drug resistance in patients on a standard thymidine analogue-based first line antiretroviral therapy after virological failure: implications for the activity of next-line regimens from a longitudinal study in Mozambique. BMC Infect Dis. 2017;17:605 pubmed publisher
    ..8 log10 copies/mL, 43 (89.6%) presented at least one resistance-associated mutation (RAM), most frequently for lamivudine/emtricitabine, nevirapine and efavirenz...
  32. Godinho A, de Oliveira Souza A, Carvalho C, Horta D, De Fraia D, Anselmo F, et al. Memory impairment due to fipronil pesticide exposure occurs at the GABAA receptor level, in rats. Physiol Behav. 2016;165:28-34 pubmed publisher
    ..Memory behavior was assessed using new object recognition task (ORT) and eight radial arm maze task (8-RAM) to study effects on cognitive and spatial memory. Locomotor behavior was assessed using open field task (OF)...
  33. Aghaz F, Hajarian H, Shabankareh H, Abdolmohammadi A. Effect of sericin supplementation in maturation medium on cumulus cell expansion, oocyte nuclear maturation, and subsequent embryo development in Sanjabi ewes during the breeding season. Theriogenology. 2015;84:1631-5 pubmed publisher
    ..After IVF with fresh ram semen, presumptive zygotes were cultured 8 days in potassium simplex optimization medium supplemented by amino ..
  34. Alvim J, Lopes B, Cass Q. Simultaneous enantioselective quantification of fluoxetine and norfluoxetine in human milk by direct sample injection using 2-dimensional liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2016;1451:120-126 pubmed publisher
    ..A restricted access media of bovine serum albumin octadecyl column (RAM-BSAC18) was used in the first dimension for the milk proteins depletion, while an antibiotic-based chiral column ..
  35. Natali C, Bianchini G, Marchina C, Knöller K. Geochemistry of the Adige River water from the Eastern Alps to the Adriatic Sea (Italy): evidences for distinct hydrological components and water-rock interactions. Environ Sci Pollut Res Int. 2016;23:11677-94 pubmed publisher
    ..TDS) concentration shows the lowest value (<100 mg/l) at the river source, jumping to 310 mg/l at the Rio Ram inflow, then decreasing down to the Isarco River confluence; from here, we observed an increase toward the river ..
  36. Jin Y, Song B, Lin C, Zhang P, Dai S, Xu T, et al. Extension of the Swanepoel method for obtaining the refractive index of chalcogenide thin films accurately at an arbitrary wavenumber. Opt Express. 2017;25:31273-31280 pubmed publisher
    ..So a regional approach method (RAM) was presented here to extend the Swanepoel method to an arbitrary wavenumber...
  37. Sherry K, Johnson S, Hatem C, Majumdar A, Barrick D. Effects of Linker Length and Transient Secondary Structure Elements in the Intrinsically Disordered Notch RAM Region on Notch Signaling. J Mol Biol. 2015;427:3587-3597 pubmed publisher
    ..Interaction of the intrinsically disordered RBP-j-associated molecule (RAM) region of NICD with CSL is thought to both disrupt binding of corepressor proteins to CSL and anchor NICD to CSL, ..
  38. Hu M, Guo G, Huang Q, Cheng C, Xu R, Li A, et al. The harsh microenvironment in infarcted heart accelerates transplanted bone marrow mesenchymal stem cells injury: the role of injured cardiomyocytes-derived exosomes. Cell Death Dis. 2018;9:357 pubmed publisher
    ..Next, we explored the role of cardiac exosomes in the survival of transplanted BMSCs in vivo by constructing a Rab27a knockout (KO) mice model by a transcription activator-like effector nuclease (TALEN) genome-editing technique; ..
  39. Zhang Y, Wen C, Liu S, Zheng L, Shen B, Tao Y. Shade avoidance 6 encodes an Arabidopsis flap endonuclease required for maintenance of genome integrity and development. Nucleic Acids Res. 2016;44:1271-84 pubmed publisher
    ..Mutant sav6 plants also show reduced root apical meristem (RAM) size and defective quiescent centre (QC) development...
  40. Li C, Fitzgerald M, Del Mar N, Cuthbertson Coates S, Ledoux M, Gong S, et al. The identification and neurochemical characterization of central neurons that target parasympathetic preganglionic neurons involved in the regulation of choroidal blood flow in the rat eye using pseudorabies virus, immunolabeling and conventional pat. Front Neuroanat. 2015;9:65 pubmed publisher
    ..part of the paraventricular nucleus of the hypothalamus (PVN), the periaqueductal gray, the raphe magnus (RaM), the B3 region of the pons, A5, the nucleus of the solitary tract (NTS), the rostral ventrolateral medulla (RVLM), ..
  41. Fole A, Miguéns M, Morales L, González Martín C, Ambrosio E, Del Olmo N. Lewis and Fischer 344 rats as a model for genetic differences in spatial learning and memory: Cocaine effects. Prog Neuropsychopharmacol Biol Psychiatry. 2017;76:49-57 pubmed publisher
    ..cocaine-induced enhanced learning in Morris water maze (MWM) highly demanding tasks, the eight-arm radial maze (RAM) performance was not modified either in LEW or F344 rats after chronic cocaine treatment...
  42. Hou Y, Ernst S, Lentz S, Williams J. Genetic deletion of Rab27B in pancreatic acinar cells affects granules size and has inhibitory effects on amylase secretion. Biochem Biophys Res Commun. 2016;471:610-5 pubmed publisher
    ..g. similar small G proteins, such as Rab3D and Rab27A, and putative downstream effectors...
  43. Kurz A, Pruenster M, Rohwedder I, Ramadass M, Schäfer K, Harrison U, et al. MST1-dependent vesicle trafficking regulates neutrophil transmigration through the vascular basement membrane. J Clin Invest. 2016;126:4125-4139 pubmed publisher
    ..Furthermore, we found that MST1 associates with the Rab27 effector protein synaptotagmin-like protein 1 (JFC1, encoded by Sytl1 in mice), but not Munc13-4, thereby ..
  44. Rouatbi M, Gharbi M, Rjeibi M, Ben Salem I, Akkari H, Lassoued N, et al. Effect of the infection with the nematode <i>Haemonchus contortus</i> (Strongylida: Trichostrongylidae) on the haematological, biochemical, clinical and reproductive traits in rams. Onderstepoort J Vet Res. 2016;83:e1-8 pubmed publisher
    ..The infected group received 30 000 H. contortus third-stage larvae orally. Each ram's ejaculate was immediately evaluated for volume, sperm cell concentration and mortality rate...
  45. Arbour J, López Fernández H. Continental cichlid radiations: functional diversity reveals the role of changing ecological opportunity in the Neotropics. Proc Biol Sci. 2016;283: pubmed publisher
    ..Across a functional morphological axis associated with ram-suction feeding traits, evolutionary rates declined through time as lineages diversified in South America...
  46. Borkar A, Vallurupalli P, Camilloni C, Kay L, Vendruscolo M. Simultaneous NMR characterisation of multiple minima in the free energy landscape of an RNA UUCG tetraloop. Phys Chem Chem Phys. 2017;19:2797-2804 pubmed publisher
    ..problem by using NMR residual dipolar couplings (RDCs) as structural restraints in replica-averaged metadynamics (RAM) simulations...
  47. Szokoli F, Castelli M, Sabaneyeva E, Schrallhammer M, Krenek S, Doak T, et al. Disentangling the Taxonomy of Rickettsiales and Description of Two Novel Symbionts ("Candidatus Bealeia paramacronuclearis" and "Candidatus Fokinia cryptica") Sharing the Cytoplasm of the Ciliate Protist Paramecium biaurelia. Appl Environ Microbiol. 2016;82:7236-7247 pubmed
    ..two groups: one represented by the families Rickettsiaceae, Anaplasmataceae, and "Candidatus Midichloriaceae" (RAM clade), and the other represented by "basal Rickettsiales," including "Candidatus Bealeia paramacronuclearis...
  48. Saxena S, Horiuchi H, Fukuda M. Rab27a regulates epithelial sodium channel (ENaC) activity through synaptotagmin-like protein (SLP-5) and Munc13-4 effector mechanism. Biochem Biophys Res Commun. 2006;344:651-7 pubmed
    ..Rab proteins are small GTPases involved in vesicle transport, docking, and fusion. Earlier, we reported that Rab27a inhibits ENaC-mediated currents through protein-protein interaction in HT-29 cells...
  49. Swelum A, Ayadi M, Alhidary I, Alowaimer A, Abouheif M. The relationships between body fatness, leptin, testosterone, and reproductive performance in ram lambs as affected by level and frequency of feeding. Theriogenology. 2017;89:79-85 pubmed publisher
    This study was carried out using 24 Najdi ram lambs of approximately 6...
  50. Salatti Dorado J, Caballero Casero N, Sicilia M, Lunar M, Rubio S. The use of a restricted access volatile supramolecular solvent for the LC/MS-MS assay of bisphenol A in urine with a significant reduction of phospholipid-based matrix effects. Anal Chim Acta. 2017;950:71-79 pubmed publisher
    Restricted access-volatile supramolecular solvents (RAM-VOL-SUPRAS) are here proposed as a new strategy for the quick removal of protein and phospholipids and efficient analyte extraction in LC-MS bioanalysis...
  51. Lee A, Lee C, Van Gelder R. Scalable metagenomics alignment research tool (SMART): a scalable, rapid, and complete search heuristic for the classification of metagenomic sequences from complex sequence populations. BMC Bioinformatics. 2016;17:292 pubmed publisher
    ..accuracy gains over Kraken at the species classification level, but SMART required approximately half the amount of RAM of CLARK...
  52. Salmon V, Castonguay F, Demers Caron V, Leclerc P, Bailey J. Cholesterol-loaded cyclodextrin improves ram sperm cryoresistance in skim milk-extender. Anim Reprod Sci. 2017;177:1-11 pubmed publisher
    Cholesterol-loaded cyclodextrin (CLC) is known to improve ram sperm cryosurvival...
  53. Uemoto R, Mizuki N. Spontaneous closure of a macular hole caused by a ruptured retinal arterial macroaneurysm. Eur J Ophthalmol. 2008;18:462-5 pubmed
    To report a spontaneous closure of a macular hole (MH) that was caused by a ruptured retinal arterial macroaneurysm (RAM).
  54. Zhang M, Bracaglia C, Prencipe G, Bemrich Stolz C, Beukelman T, Dimmitt R, et al. A Heterozygous RAB27A Mutation Associated with Delayed Cytolytic Granule Polarization and Hemophagocytic Lymphohistiocytosis. J Immunol. 2016;196:2492-503 pubmed publisher
    ..We report two unrelated teenagers with HLH and an identical heterozygous RAB27A mutation (c.259G→C). We explore the contribution of this Rab27A missense (p...
  55. Lee M, Cullen N, Newman S, Dodds K, McEwan J, Shackell G. Genetic analysis and genomic selection of stayability and productive life in New Zealand ewes. J Anim Sci. 2015;93:3268-77 pubmed publisher
    ..Records from 658,871 animals from 241 seed stock or ram breeder flocks and one research flock were used to estimate parameters for ram breeder flock STAY and Prodlife...
  56. Fukuda M. Synaptotagmin-like protein (Slp) homology domain 1 of Slac2-a/melanophilin is a critical determinant of GTP-dependent specific binding to Rab27A. J Biol Chem. 2002;277:40118-24 pubmed
    ..protein (Slp) homology domain (SHD) of the Slp and Slac2 families has recently been identified as a specific Rab27A-binding domain (Kuroda, T. S., Fukuda, M., Ariga, H., and Mikoshiba, K. (2002) J. Biol. Chem...
  57. van Breevoort D, Snijders A, Hellen N, Weckhuysen S, van Hooren K, Eikenboom J, et al. STXBP1 promotes Weibel-Palade body exocytosis through its interaction with the Rab27A effector Slp4-a. Blood. 2014;123:3185-94 pubmed publisher
    ..We have shown that the Rab27A effector synaptotagmin-like protein 4-a (Slp4-a) plays a critical role in regulating hormone-evoked WPB exocytosis...
  58. Zou L, Zhou J, Zhang J, Li J, Liu N, Chai L, et al. The GTPase Rab3b/3c-positive recycling vesicles are involved in cross-presentation in dendritic cells. Proc Natl Acad Sci U S A. 2009;106:15801-6 pubmed publisher
    ..Furthermore, the Rab3b/3c-positive compartments were colocalized with a fraction of Rab27a at a juxtaposition of phagosomes...
  59. Yoshida Amano Y, Hachiya A, Ohuchi A, Kobinger G, Kitahara T, Takema Y, et al. Essential role of RAB27A in determining constitutive human skin color. PLoS ONE. 2012;7:e41160 pubmed publisher
    ..Mutations in genes encoding melanosome transport-related molecules, such as MYO5A, RAB27A and SLAC-2A, have been reported to cause a human pigmentary disease known as Griscelli syndrome, which is ..
  60. Ferreira M, Vale D, Cunha L, Melo P. Role of the C-terminal extension peptide of plastid located glutamine synthetase from Medicago truncatula: Crucial for enzyme activity and needless for protein import into the plastids. Plant Physiol Biochem. 2017;111:226-233 pubmed publisher
    ..is encoded by a small family of highly homologous nuclear genes that produce cytosolic (GS1) and plastidic (GS2) isoforms...
  61. Worst T, Meyer Y, Gottschalt M, Weis C, von Hardenberg J, Frank C, et al. RAB27A, RAB27B and VPS36 are downregulated in advanced prostate cancer and show functional relevance in prostate cancer cells. Int J Oncol. 2017;50:920-932 pubmed publisher
    ..involvement in vesicular trafficking and secretion in an existing microarray dataset revealed low expression of RAB27A, RAB27B and VPS36 to be predictive for reduced BCR-free survival in patients with localized PCa (p=0.033, 0...
  62. Cronin T, Fasick J, Schweikert L, Johnsen S, Kezmoh L, Baumgartner M. Coping with copepods: do right whales (Eubalaena glacialis) forage visually in dark waters?. Philos Trans R Soc Lond B Biol Sci. 2017;372: pubmed publisher
    ..food primarily consists of planktonic copepods, Calanus finmarchicus, which they consume in large numbers by ram filter feeding...
  63. Li X, Ye J, Ma H, Lu P. Proteomic analysis of lysine acetylation provides strong evidence for acetylated proteins involved in plant meiosis and tapetum function. Plant J. 2017;: pubmed publisher
    ..from a proteomic investigation to detect KAC status of the developing rice anthers near the time of meiosis (RAM), providing strong biochemical evidence for roles of many KAC-affected proteins during rice anther development and ..
  64. Muller A, Hundt C, Hildebrandt A, Hankeln T, Schmidt B. MetaCache: Context-aware classification of metagenomic reads using minhashing. Bioinformatics. 2017;: pubmed publisher
    ..of memory while both Kraken and CLARK fail to construct their respective databases on a workstation with 512?GB RAM. Our experimental results further show that classification accuracy continuously improves when increasing the ..
  65. Wu X, Sakamoto T, Zhang F, Sellers J, Hammer J. In vitro reconstitution of a transport complex containing Rab27a, melanophilin and myosin Va. FEBS Lett. 2006;580:5863-8 pubmed
    b>Rab27a and melanophilin (Mlph) are required in vivo to form a melanosome receptor for myosin Va in which Rab27a anchored in the melanosome membrane recruits Mlph, which in turn recruits myosin Va...
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    ..trafficking and found that ferritin secretion was decreased by a BLOC-1 mutation but increased by BLOC-2, -3 and Rab27A mutations of the cellular trafficking machinery, suggesting multiple export routes...
  67. Hussein A, Aher Y, Kalaba P, Aher N, Dragačević V, Radoman B, et al. A novel heterocyclic compound improves working memory in the radial arm maze and modulates the dopamine receptor D1R in frontal cortex of the Sprague-Dawley rat. Behav Brain Res. 2017;332:308-315 pubmed publisher
    ..and effective dopamine re-uptake inhibition DAT (IC50=4,1±0,8?M) made us test this compound in a radial arm maze (RAM) in the rat...
  68. Li Y, Chen S, Shan Z, Bi L, Yu S, Li Y, et al. miR-182-5p improves the viability, mitosis, migration, and invasion ability of human gastric cancer cells by down-regulating RAB27A. Biosci Rep. 2017;37: pubmed publisher
    ..viability, proliferation, invasion, and migration ability of human gastric cells by regulating the expression of RAB27A. Real-time PCR assay was used to detect the expression of miR-182-5 and RAB27A in human gastric carcinoma tissues, ..
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    ..In most Griscelli patients, the RAB27A gene, which encodes a small GTPase that is associated with the melanosome membrane in melanocytes, is mutated...
  70. Torii S, Zhao S, Yi Z, Takeuchi T, Izumi T. Granuphilin modulates the exocytosis of secretory granules through interaction with syntaxin 1a. Mol Cell Biol. 2002;22:5518-26 pubmed
    ..A novel set of Rab and its effector, Rab27a/granuphilin, which is localized on insulin granules in pancreatic beta cells, was recently identified...
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    ..However, early mortality and recurrence after surgery were still of major concern. RAM (Risk Assessment for early Mortality) scoring system is a newly developed tool for assessing early mortality after ..
  72. Meo A, Chureemart P, Wang S, Chepulskyy R, Apalkov D, Chantrell R, et al. Thermally nucleated magnetic reversal in CoFeB/MgO nanodots. Sci Rep. 2017;7:16729 pubmed publisher
    ..Magnetic RAM (MRAM) with CoFeB/MgO based magnetic tunnel junctions (MTJs) is a promising candidate for reducing the power ..
  73. Bahadoran P, Busca R, Chiaverini C, Westbroek W, Lambert J, Bille K, et al. Characterization of the molecular defects in Rab27a, caused by RAB27A missense mutations found in patients with Griscelli syndrome. J Biol Chem. 2003;278:11386-92 pubmed
    b>Rab27a plays a pivotal role in the transport of melanosomes to dendrite tips of melanocytes and mutations in RAB27A, which impair melanosome transport cause the pigmentary dilution and the immune deficiency found in several patients with ..
  74. Li X, Wang H, Ni Q, Tang Z, Ni J, Xu L, et al. Effects of silencing Rab27a gene on biological characteristics and chemosensitivity of non-small cell lung cancer. Oncotarget. 2017;8:94481-94492 pubmed publisher
    b>Rab27a, a member of the Rab protein family, can regulate the tumor microenvironment and promote the development of the tumor...
  75. Wood S, Meeths M, Chiang S, Bechensteen A, Boelens J, Heilmann C, et al. Different NK cell-activating receptors preferentially recruit Rab27a or Munc13-4 to perforin-containing granules for cytotoxicity. Blood. 2009;114:4117-27 pubmed publisher
    ..and familial hemophagocytic lymphohistiocytosis type 3 (FHL3) are associated with loss-of-function mutations in RAB27A (encoding Rab27a) and UNC13D (encoding Munc13-4)...
  76. Rui B, Angrimani D, Bicudo L, Losano J, Nichi M, Pereira R. A fast, low-cost and efficient method for the diagnosis of sperm DNA fragmentation in several species. Reprod Domest Anim. 2017;: pubmed publisher
    ..the toluidine blue (TB) stain technique for the analysis of DNA fragmentation of dog, cat, bull, stallion and ram spermatozoa. For this purpose, we used six animals of each specie (n = 30), in reproductive age...
  77. Masri A, Bakri F, Al Hussaini M, Al Hadidy A, Hirzallah R, De Saint Basile G, et al. Griscelli syndrome type 2: a rare and lethal disorder. J Child Neurol. 2008;23:964-7 pubmed publisher
    ..Mutation analysis in family members revealed the presence of a missense mutation in Rab27a gene...
  78. Fini M, Bauskar A, Jeong S, Wilson M. Clusterin in the eye: An old dog with new tricks at the ocular surface. Exp Eye Res. 2016;147:57-71 pubmed publisher
    The multifunctional protein clusterin (CLU) was first described in 1983 as a secreted glycoprotein present in ram rete testis fluid that enhanced aggregation ('clustering') of a variety of cells in vitro...
  79. Schönhals A, Waser R, Wouters D. Improvement of SET variability in TaO x based resistive RAM devices. Nanotechnology. 2017;28:465203 pubmed publisher
    ..The impact of voltage divider on SET variability is demonstrated. A combination of appropriate write voltage and serial resistance allows for a significant improvement of the SET variability...
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    ..the average activity during the most active 10-h period (M10) were used to calculate the relative amplitude mean [RAm = (M10-L5)/(M10+L5)]. COPD patients presented higher IVm (0.242 ± 0.097 vs 0.182 ± 0.063) and L5 (36...
  81. Iga K, Kiriyama A. Usefulness of Two-Compartment Model-Assisted and Static Overall Inhibitory-Activity Method for Prediction of Drug-Drug Interaction. Biol Pharm Bull. 2017;: pubmed publisher
    ..of drug-drug interaction (DDI) started with the clarification of unusually large DDI observed between ramelteon (RAM) and fluvoxamine (FLV)...
  82. Prasad S, Bhatia T, Kukshal P, Nimgaonkar V, Deshpande S, Thelma B. Attempts to replicate genetic associations with schizophrenia in a cohort from north India. NPJ Schizophr. 2017;3:28 pubmed publisher
    ..Researchers led by the University of Delhi's BK Thelma, and Smita Deshpande of the Dr. Ram Manohar Lohia Hospital, India, selected 32 gene variations from past studies to look for similar associations in ..
  83. Rak A, Pylypenko O, Niculae A, Pyatkov K, Goody R, Alexandrov K. Structure of the Rab7:REP-1 complex: insights into the mechanism of Rab prenylation and choroideremia disease. Cell. 2004;117:749-60 pubmed
    ..Using semisynthetic fluorescent Rab27A, we demonstrate that although Rab27A can be prenylated by REP-2, this reaction can be effectively inhibited by ..
  84. Esposito G, Ana Clara F, Verstreken P. Synaptic vesicle trafficking and Parkinson's disease. Dev Neurobiol. 2012;72:134-44 pubmed publisher
    ..This article reviews our current understanding for a role of causative PD genes in the SV cycle and speculates on the potential role of aberrant SV trafficking in the neurodegenerative cascade of PD. ..
  85. Elstak E, Neeft M, Nehme N, Voortman J, Cheung M, Goodarzifard M, et al. The munc13-4-rab27 complex is specifically required for tethering secretory lysosomes at the plasma membrane. Blood. 2011;118:1570-8 pubmed publisher
    ..munc13-4 binds rab27a, but the necessity of the complex remains enigmatic, because studies in knockout models suggest separate functions...
  86. Rojo Pulido I, Nightingale T, Darchen F, Seabra M, Cutler D, Gerke V. Myosin Va acts in concert with Rab27a and MyRIP to regulate acute von-Willebrand factor release from endothelial cells. Traffic. 2011;12:1371-82 pubmed publisher
    ..The WPB-associated small GTPase Rab27a is involved in restricting immature WPB exocytosis and we searched for links between Rab27a and the actin ..
  87. Pylypenko O, Attanda W, Gauquelin C, Lahmani M, Coulibaly D, Baron B, et al. Structural basis of myosin V Rab GTPase-dependent cargo recognition. Proc Natl Acad Sci U S A. 2013;110:20443-8 pubmed publisher
    ..compartments via Rab11 or to the melanosome membrane via recognition of the melanophilin adaptor that binds to Rab27a. The structures illustrate how the Rab11-binding site has been conserved during evolution and how divergence at ..
  88. Menasche G, Feldmann J, Houdusse A, Desaymard C, Fischer A, Goud B, et al. Biochemical and functional characterization of Rab27a mutations occurring in Griscelli syndrome patients. Blood. 2003;101:2736-42 pubmed
    b>Rab27a is a member of the Rab family of small GTPase proteins, and thus far is the first member to be associated with a human disease (ie, the Griscelli syndrome type 2)...
  89. Gerber P, Cabrini M, Jancic C, Paoletti L, Banchio C, von Bilderling C, et al. Rab27a controls HIV-1 assembly by regulating plasma membrane levels of phosphatidylinositol 4,5-bisphosphate. J Cell Biol. 2015;209:435-52 pubmed publisher
    ..We show that Rab27a controls the trafficking of late endosomes carrying phosphatidylinositol 4-kinase type 2 α (PI4KIIα) toward ..
  90. Zhang X, Zhang Y, Yang J, Li S, Chen J. Upregulation of miR-582-5p inhibits cell proliferation, cell cycle progression and invasion by targeting Rab27a in human colorectal carcinoma. Cancer Gene Ther. 2015;22:475-80 pubmed publisher
    ..Further analysis demonstrated that miR-582-5p attenuated the expression of RAS-related GTP-binding protein (Rab27a). Luciferase reporter assay confirmed that Rab27a was a target of miR-582-5p...