PRMT3

Summary

Gene Symbol: PRMT3
Description: protein arginine methyltransferase 3
Alias: HRMT1L3, protein arginine N-methyltransferase 3, HMT1 hnRNP methyltransferase-like 3, heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
Species: human
Products:     PRMT3

Top Publications

  1. Mowen K, Tang J, Zhu W, Schurter B, Shuai K, Herschman H, et al. Arginine methylation of STAT1 modulates IFNalpha/beta-induced transcription. Cell. 2001;104:731-41 pubmed
  2. Bachand F, Silver P. PRMT3 is a ribosomal protein methyltransferase that affects the cellular levels of ribosomal subunits. EMBO J. 2004;23:2641-50 pubmed
    The mammalian protein arginine methyltransferase 3 (PRMT3) catalyzes the formation of asymmetric (type I) dimethylarginine in vitro. As yet, natural substrates and cellular pathways modulated by PRMT3 remain unknown...
  3. Fronz K, Otto S, Kölbel K, Kuhn U, Friedrich H, Schierhorn A, et al. Promiscuous modification of the nuclear poly(A)-binding protein by multiple protein-arginine methyltransferases does not affect the aggregation behavior. J Biol Chem. 2008;283:20408-20 pubmed publisher
    ..Nevertheless, PABPN1 was almost fully methylated in a Prmt1(-/-) cell line; thus, PRMT3 and -6 suffice for methylation...
  4. Singh V, Miranda T, Jiang W, Frankel A, Roemer M, Robb V, et al. DAL-1/4.1B tumor suppressor interacts with protein arginine N-methyltransferase 3 (PRMT3) and inhibits its ability to methylate substrates in vitro and in vivo. Oncogene. 2004;23:7761-71 pubmed
    ..domain as bait for yeast two-hybrid interaction cloning, we identified protein arginine N-methyltransferase 3 (PRMT3) as a specific DAL-1/4.1B-interacting protein...
  5. Jiang W, Roemer M, Newsham I. The tumor suppressor DAL-1/4.1B modulates protein arginine N-methyltransferase 5 activity in a substrate-specific manner. Biochem Biophys Res Commun. 2005;329:522-30 pubmed
    ..Using yeast two-hybrid interaction cloning, protein arginine N-methyltransferase 3 (PRMT3) was originally identified as a DAL-1/4.1B-interacting protein...
  6. Smith J, Rücknagel K, Schierhorn A, Tang J, Nemeth A, Linder M, et al. Unusual sites of arginine methylation in Poly(A)-binding protein II and in vitro methylation by protein arginine methyltransferases PRMT1 and PRMT3. J Biol Chem. 1999;274:13229-34 pubmed
    ..in Escherichia coli were in vitro substrates for two mammalian protein arginine methyltransferases, PRMT1 and PRMT3, with S-adenosyl-L-methionine as the methyl group donor...
  7. Tang J, Gary J, Clarke S, Herschman H. PRMT 3, a type I protein arginine N-methyltransferase that differs from PRMT1 in its oligomerization, subcellular localization, substrate specificity, and regulation. J Biol Chem. 1998;273:16935-45 pubmed
    ..A recombinant glutathione S-transferase (GST) fusion product of this new rat protein, named PRMT3, asymmetrically dimethylates arginine residues present both in the designed substrate GST-GAR and in substrate ..
  8. Hoekstra M, Nahon J, de Jong L, Kröner M, de Leeuw L, Van Eck M. Inhibition of PRMT3 activity reduces hepatic steatosis without altering atherosclerosis susceptibility in apoE knockout mice. Biochim Biophys Acta Mol Basis Dis. 2019;: pubmed publisher
    ..It is proposed that inhibition of the protein arginine methyltransferase 3 (PRMT3) uncouples these two transcriptional pathways in vivo by acting as a specific lipogenic coactivator of LXR...
  9. Dionne K, Bergeron D, Landry Voyer A, Bachand F. The 40S ribosomal protein uS5 (RPS2) assembles into an extraribosomal complex with human ZNF277 that competes with the PRMT3-uS5 interaction. J Biol Chem. 2019;294:1944-1955 pubmed publisher
    ..protein uS5 (RPS2) is known to form an extraribosomal complex with the protein arginine methyltransferase PRMT3 that is conserved from fission yeast to humans...

More Information

Publications44

  1. Hsu M, Pan M, Chu P, Tsai Y, Tsai C, Shan Y, et al. Protein Arginine Methyltransferase 3 Enhances Chemoresistance in Pancreatic Cancer by Methylating hnRNPA1 to Increase ABCG2 Expression. Cancers (Basel). 2018;11: pubmed publisher
    ..Our previous study showed that epigenetic modifying enzymes including protein arginine methyltransferase 3 (PRMT3) are dysregulated in gemcitabine (GEM)-resistant pancreatic cancer cells...
  2. Hang R, Liu C, Ahmad A, Zhang Y, Lu F, Cao X. Arabidopsis protein arginine methyltransferase 3 is required for ribosome biogenesis by affecting precursor ribosomal RNA processing. Proc Natl Acad Sci U S A. 2014;111:16190-5 pubmed publisher
    ..Here we show that Arabidopsis PRMT3 (AtPRMT3) is required for ribosome biogenesis by affecting pre-rRNA processing...
  3. Wang Y, Wang J, Chen C, Chen Y, Li C. A novel BLAST-Based Relative Distance (BBRD) method can effectively group members of protein arginine methyltransferases and suggest their evolutionary relationship. Mol Phylogenet Evol. 2015;84:101-11 pubmed publisher
    ..with PRMT7 in the same clade, outside of all the Type I PRMTs including PRMT1 and its vertebrate paralogue PRMT8, PRMT3, PRMT6, PRMT2 and PRMT4. The PRMT7/9 branch then connects with the type II PRMT5...
  4. Park M, Kim D, Choi J, Heo Y, Park S. New role of irisin in hepatocytes: The protective effect of hepatic steatosis in vitro. Cell Signal. 2015;27:1831-9 pubmed publisher
    ..against lipogenesis and oxidative stress were mediated by the inhibition of protein arginine methyltransferase-3 (PRMT3)...
  5. Xu H, Xu S, Mo K. [Transcription of protein arginine N-methyltransferase genes in mouse dorsal root ganglia following peripheral nerve injury]. Nan Fang Yi Ke Da Xue Xue Bao. 2017;37:1620-1625 pubmed
    ..9 protein arginine methylation enzyme family genes that were tissue?specifically expressed in the DRG, Prmt2 and Prmt3 showed the highest and Prmt6 showed the lowest basal expression...
  6. Smith C, Anthony S, Hubank M, Leiper J, Vallance P. Effects of ADMA upon gene expression: an insight into the pathophysiological significance of raised plasma ADMA. PLoS Med. 2005;2:e264 pubmed
    ..5 (Smad5), bone morphogenetic protein receptor 1A, and protein arginine methyltransferase 3 (PRMT3; also known as HRMT1L3), were confirmed by Northern blotting, quantitative PCR, and in some instances Western blotting analysis to ..
  7. Winter D, Hart Smith G, Wilkins M. Characterization of Protein Methyltransferases Rkm1, Rkm4, Efm4, Efm7, Set5 and Hmt1 Reveals Extensive Post-Translational Modification. J Mol Biol. 2018;430:102-118 pubmed publisher
    ..These may regulate MTase activity. Finally, we compared PTMs on Hmt1 with those on its human homologs PRMT1, PRMT3, CARM1, PRMT6 and PRMT8...
  8. Fulton M, Zhang J, He M, Ho M, Zheng Y. Intricate Effects of ?-Amino and Lysine Modifications on Arginine Methylation of the N-Terminal Tail of Histone H4. Biochemistry. 2017;56:3539-3548 pubmed publisher
    ..Our studies found that acylations of H4K5 resulted in decreased levels of arginine methylation by PRMT1, PRMT3, and PRMT8...
  9. Liu F, Li F, Ma A, Dobrovetsky E, Dong A, Gao C, et al. Exploiting an allosteric binding site of PRMT3 yields potent and selective inhibitors. J Med Chem. 2013;56:2110-24 pubmed publisher
    ..Among the nine known human PRMTs, PRMT3 has been implicated in ribosomal biosynthesis via asymmetric dimethylation of the 40S ribosomal protein S2 and in ..
  10. Campbell M, Chang P, Huerta S, Izumiya C, Davis R, Tepper C, et al. Protein arginine methyltransferase 1-directed methylation of Kaposi sarcoma-associated herpesvirus latency-associated nuclear antigen. J Biol Chem. 2012;287:5806-18 pubmed publisher
    ..These results suggest that residue 20 is important for modulation of a subset of LANA functions and properties of this residue, including the hydrophobic character induced by arginine methylation, may contribute to the observed effects. ..
  11. Jiang W, Newsham I. The tumor suppressor DAL-1/4.1B and protein methylation cooperate in inducing apoptosis in MCF-7 breast cancer cells. Mol Cancer. 2006;5:4 pubmed
    ..However the exact mechanism of action of DAL-1/4.1B is unknown. Recently, protein arginine N-methyltransferase 3 (PRMT3) was identified as a DAL-1/4.1B interacting protein...
  12. Landry Voyer A, Bilodeau S, Bergeron D, Dionne K, Port S, Rouleau C, et al. Human PDCD2L Is an Export Substrate of CRM1 That Associates with 40S Ribosomal Subunit Precursors. Mol Cell Biol. 2016;36:3019-3032 pubmed
    Protein arginine methyltransferase 3 (PRMT3) forms a stable complex with 40S ribosomal protein S2 (RPS2) and contributes to ribosome biogenesis...
  13. Kim C, Lim Y, Yoo B, Won N, Kim S, Kim G. Regulation of post-translational protein arginine methylation during HeLa cell cycle. Biochim Biophys Acta. 2010;1800:977-85 pubmed publisher
    ..ASYM24), anti-symmetric-dimethyl-arginine (SYM10), and subclasses of PRMTs, including PRMT1, PRMT3, PRMT4 (CARM1), PRMT5, PRMT6, and PRMT7 antibodies...
  14. Choi S, Jung C, Kim J, Im D. PRMT3 inhibits ubiquitination of ribosomal protein S2 and together forms an active enzyme complex. Biochim Biophys Acta. 2008;1780:1062-9 pubmed publisher
    Protein arginine methyltransferase 3 (PRMT3) comprises a region not required for catalytic activity in its amino-terminus and the core domain catalyzing protein arginine methylation...
  15. Ma M, Zhao X, Chen S, Zhao Y, Yang L, Feng Y, et al. Strategy Based on Deglycosylation, Multiprotease, and Hydrophilic Interaction Chromatography for Large-Scale Profiling of Protein Methylation. Anal Chem. 2017;: pubmed publisher
    ..regulation of several putative methylated sites that are related to the protein arginine N-methyltransferase 3 (PRMT3)...
  16. Blanc R, Richard S. Arginine Methylation: The Coming of Age. Mol Cell. 2017;65:8-24 pubmed publisher
    ..This review describes the recent molecular advances that have been uncovered in normal and diseased mammalian cells. ..
  17. Kölbel K, Ihling C, Bellmann Sickert K, Neundorf I, Beck Sickinger A, Sinz A, et al. Type I Arginine Methyltransferases PRMT1 and PRMT-3 Act Distributively. J Biol Chem. 2009;284:8274-82 pubmed publisher
    ..Even though the reaction is distributive, the efficiency of methylation of one particular protein strongly depends on the number of methyl-accepting arginine residues it contains. ..
  18. Swiercz R, Person M, Bedford M. Ribosomal protein S2 is a substrate for mammalian PRMT3 (protein arginine methyltransferase 3). Biochem J. 2005;386:85-91 pubmed
    b>PRMT3 (protein arginine methyltransferase 3) is one of four type I arginine methyltransferases that catalyse the formation of asymmetric dimethylarginine...
  19. Dolzhanskaya N, Merz G, Denman R. Alternative splicing modulates protein arginine methyltransferase-dependent methylation of fragile X syndrome mental retardation protein. Biochemistry. 2006;45:10385-93 pubmed
    ..protein arginine methyltransferases (PRMTs), we showed that the C-terminal domain could be methylated by PRMT1, PRMT3, and PRMT4 in vitro and that both the R(544)-K mutant and the R(546)-K mutant were refractory toward these enzymes...
  20. Zou Y, Webb K, Perna A, Zhang Q, Clarke S, Wang Y. A mass spectrometric study on the in vitro methylation of HMGA1a and HMGA1b proteins by PRMTs: methylation specificity, the effect of binding to AT-rich duplex DNA, and the effect of C-terminal phosphorylation. Biochemistry. 2007;46:7896-906 pubmed
    ..human HMGA1a and HMGA1b proteins by three members of the protein arginine methyltransferase (PRMT) family: PRMT1, PRMT3, and PRMT6...
  21. Kaniskan H, Eram M, Zhao K, Szewczyk M, Yang X, Schmidt K, et al. Discovery of Potent and Selective Allosteric Inhibitors of Protein Arginine Methyltransferase 3 (PRMT3). J Med Chem. 2017;: pubmed publisher
    b>PRMT3 catalyzes the asymmetric dimethylation of arginine residues of various proteins. It is crucial for maturation of ribosomes, and has been implicated in several diseases...
  22. Dolzhanskaya N, Bolton D, Denman R. Chemical and structural probing of the N-terminal residues encoded by FMR1 exon 15 and their effect on downstream arginine methylation. Biochemistry. 2008;47:8491-503 pubmed publisher
    ..refractory toward methylation; however, it can be methylated by supplementing extracts with recombinant PRMT1 or PRMT3. Protein modeling studies reveal that the RG-rich region is part of a three to four strand antiparallel beta-sheet,..
  23. Chan J, Hsieh T, Liu S, Chou W, Chung M, Huang S. Physical and functional interactions between hnRNP K and PRMT family proteins. FEBS Lett. 2009;583:281-6 pubmed publisher
    ..We believe these observations will help provide insights into the regulation of hnRNP K protein functions via the recruitment of its associated proteins, including its arginine methylation-modifying proteins...
  24. Siarheyeva A, Senisterra G, Allali Hassani A, Dong A, Dobrovetsky E, Wasney G, et al. An allosteric inhibitor of protein arginine methyltransferase 3. Structure. 2012;20:1425-35 pubmed publisher
    b>PRMT3, a protein arginine methyltransferase, has been shown to influence ribosomal biosynthesis by catalyzing the dimethylation of the 40S ribosomal protein S2...
  25. Handrkova H, Petrak J, Halada P, Pospisilova D, Cmejla R. Tyrosine 87 is vital for the activity of human protein arginine methyltransferase 3 (PRMT3). Biochim Biophys Acta. 2011;1814:277-82 pubmed publisher
    Protein arginine methyltransferase 3 (PRMT3) is a cytosolic enzyme that catalyzes the formation of mono- and asymmetric dimethyl arginines, with ribosomal protein (RP) S2 as its main in vivo substrate...
  26. Kim D, Park M, Lim S, Park J, Yoon K, Han H, et al. PRMT3 regulates hepatic lipogenesis through direct interaction with LXRα. Diabetes. 2015;64:60-71 pubmed publisher
    ..In this study, palmitic acid (PA) treatment increased PRMT3, which is correlated with the elevation of hepatic lipogenic proteins...
  27. Guo H, Wang R, Zheng W, Chen Y, Blum G, Deng H, et al. Profiling substrates of protein arginine N-methyltransferase 3 with S-adenosyl-L-methionine analogues. ACS Chem Biol. 2014;9:476-84 pubmed publisher
    Protein arginine N-methyltransferase 3 (PRMT3) belongs to the family of type I PRMTs and harbors the activity to use S-adenosyl-l-methionine (SAM) as a methyl-donor cofactor for protein arginine labeling...
  28. Ravenscroft T, Baker M, Rutherford N, Neumann M, Mackenzie I, Josephs K, et al. Mutations in protein N-arginine methyltransferases are not the cause of FTLD-FUS. Neurobiol Aging. 2013;34:2235.e11-3 pubmed publisher
    ..is the result of mutations in protein N-arginine methyltransferases (PRMTs), we selected 3 candidate genes (PRMT1, PRMT3, and PRMT8) and performed complete sequencing analysis and real-time polymerase chain reaction mRNA expression ..
  29. Wang R, Ibáñez G, Islam K, Zheng W, Blum G, Sengelaub C, et al. Formulating a fluorogenic assay to evaluate S-adenosyl-L-methionine analogues as protein methyltransferase cofactors. Mol Biosyst. 2011;7:2970-81 pubmed publisher
    ..83-0.95 for the so-far-examined 8 human PMTs with SAM as a cofactor (PRMT1, PRMT3, CARM1, SUV39H2, SET7/9, SET8, G9a and GLP1)...
  30. Guo Q, Bedford M, Fast W. Discovery of peptidylarginine deiminase-4 substrates by protein array: antagonistic citrullination and methylation of human ribosomal protein S2. Mol Biosyst. 2011;7:2286-95 pubmed publisher
    ..region of RPS2, which is also an established site for Arg methylation by protein arginine methyltransferase 3 (PRMT3)...
  31. Chen X, Niroomand F, Liu Z, Zankl A, Katus H, Jahn L, et al. Expression of nitric oxide related enzymes in coronary heart disease. Basic Res Cardiol. 2006;101:346-53 pubmed
    ..The expression of PRMT1 and PRMT3 was increased...
  32. Sack J, Thieffine S, Bandiera T, Fasolini M, Duke G, Jayaraman L, et al. Structural basis for CARM1 inhibition by indole and pyrazole inhibitors. Biochem J. 2011;436:331-9 pubmed publisher
    ..select residues that interact with the inhibitors may be responsible for the CARM1 selectivity against PRMT1 and PRMT3. Together, the structural and biophysical information should aid in the design of both potent and specific ..
  33. Tavanez J, Bengoechea R, Berciano M, Lafarga M, Carmo Fonseca M, Enguita F. Hsp70 chaperones and type I PRMTs are sequestered at intranuclear inclusions caused by polyalanine expansions in PABPN1. PLoS ONE. 2009;4:e6418 pubmed publisher
    ..Pull-down assays show that heat shock proteins including Hsp70, and type I arginine methyl transferases (PRMT1 and PRMT3) associate preferentially with expanded PABPN1...
  34. Beltran Alvarez P, Espejo A, Schmauder R, BELTRAN C, Mrowka R, Linke T, et al. Protein arginine methyl transferases-3 and -5 increase cell surface expression of cardiac sodium channel. FEBS Lett. 2013;587:3159-65 pubmed publisher
    ..5 current density by enhancing NaV1.5 cell surface expression. Our observations are the first evidence of regulation of a voltage-gated ion channel, including calcium, potassium, sodium and TRP channels, by arginine methylation. ..
  35. Miranda T, Webb K, Edberg D, Reeves R, Clarke S. Protein arginine methyltransferase 6 specifically methylates the nonhistone chromatin protein HMGA1a. Biochem Biophys Res Commun. 2005;336:831-5 pubmed
    ..Although GST fusions of PRMT1 and PRMT3 were also capable of methylating the full-length HMGA1a polypeptide, they recognize its proteolytic degradation ..