Gene Symbol: PRKACA
Description: protein kinase cAMP-activated catalytic subunit alpha
Alias: PKACA, PPNAD4, cAMP-dependent protein kinase catalytic subunit alpha, PKA C-alpha, protein kinase A catalytic subunit, protein kinase, cAMP-dependent, alpha catalytic subunit, protein kinase, cAMP-dependent, catalytic, alpha
Species: human
Products:     PRKACA

Top Publications

  1. Yoon C, Korade Z, Carter B. Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype. J Neurosci. 2008;28:3738-46 pubmed publisher
  2. Razani B, Lisanti M. Two distinct caveolin-1 domains mediate the functional interaction of caveolin-1 with protein kinase A. Am J Physiol Cell Physiol. 2001;281:C1241-50 pubmed
    ..e., the mutant acts in a dominant-negative fashion. Thus, this dominant-negative caveolin-1 mutant will be extremely valuable for assessing the functional role of endogenous caveolin-1 in regulating a variety of other signaling cascades...
  3. Shirakawa K, Takaori Kondo A, Yokoyama M, Izumi T, Matsui M, Io K, et al. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Nat Struct Mol Biol. 2008;15:1184-91 pubmed publisher
    ..These data imply that PKA-mediated phosphorylation of A3G can regulate the interaction between A3G and Vif. ..
  4. Desseyn J, Burton K, McKnight G. Expression of a nonmyristylated variant of the catalytic subunit of protein kinase A during male germ-cell development. Proc Natl Acad Sci U S A. 2000;97:6433-8 pubmed
    ..Because protein kinase A is thought to play a pivotal role in sperm motility and capacitation, the distinctive biochemical properties of the unmyristylated Calpha2 may be essential for fertility in the male. ..
  5. Chang C, Chuang H, Yu C, Yao T, Chen H. Stimulation of GCMa transcriptional activity by cyclic AMP/protein kinase A signaling is attributed to CBP-mediated acetylation of GCMa. Mol Cell Biol. 2005;25:8401-14 pubmed
    ..Our results reveal a novel regulation of GCMa activity by cAMP-dependent protein acetylation and provide a molecular mechanism by which cAMP signaling regulates trophoblastic fusion. ..
  6. Guan H, Hou S, Ricciardi R. DNA binding of repressor nuclear factor-kappaB p50/p50 depends on phosphorylation of Ser337 by the protein kinase A catalytic subunit. J Biol Chem. 2005;280:9957-62 pubmed
    ..Here, we report that p50 Ser(337) is constitutively phosphorylated by the protein kinase A catalytic subunit (PKAc) in three different cell types, which may account for the constant binding of p50/p50 to DNA ..
  7. Zhang F, Hu Y, Huang P, Toleman C, Paterson A, Kudlow J. Proteasome function is regulated by cyclic AMP-dependent protein kinase through phosphorylation of Rpt6. J Biol Chem. 2007;282:22460-71 pubmed
    ..Thus, hormones using the PKA system can also regulate proteasomes often in concert with glucose metabolism. This finding might lead to novel strategies for the treatment of proteasome-related diseases. ..
  8. Barnitz R, Wan F, Tripuraneni V, Bolton D, Lenardo M. Protein kinase A phosphorylation activates Vpr-induced cell cycle arrest during human immunodeficiency virus type 1 infection. J Virol. 2010;84:6410-24 pubmed publisher
    ..Inhibition of PKA activity during HIV-1 infection abrogates Vpr cell cycle arrest. These findings provide new insight into the signaling event that activates Vpr cell cycle arrest, ultimately leading to the death of infected T cells. ..
  9. Kvissel A, Ørstavik S, Eikvar S, Brede G, Jahnsen T, Collas P, et al. Involvement of the catalytic subunit of protein kinase A and of HA95 in pre-mRNA splicing. Exp Cell Res. 2007;313:2795-809 pubmed
    ..Our findings demonstrate that the nuclear PKA C subunit co-locates with HA95 in SFCs and regulates pre-mRNA splicing, possibly through a cAMP-independent mechanism. ..

More Information


  1. Li P, Wang T, Buckley K, Chenine A, Popov S, Ruprecht R. Phosphorylation of HIV Nef by cAMP-dependent protein kinase. Virology. 2005;331:367-74 pubmed
    ..As this mutation played a major role in abrogating the Nef effect on HIV replication in unstimulated primary cells, we postulate that Nef phosphorylation by PKA is an important step in the viral life cycle in resting cells. ..
  2. Hou S, Guan H, Ricciardi R. Phosphorylation of serine 337 of NF-kappaB p50 is critical for DNA binding. J Biol Chem. 2003;278:45994-8 pubmed
    ..This study shows for the first time that the DNA binding ability of NF-kappaB p50 subunit is regulated through phosphorylation of residue Ser337, which has implications for both positive and negative control of NF-kappaB transcription. ..
  3. Cartier C, Hémonnot B, Gay B, Bardy M, Sanchiz C, Devaux C, et al. Active cAMP-dependent protein kinase incorporated within highly purified HIV-1 particles is required for viral infectivity and interacts with viral capsid protein. J Biol Chem. 2003;278:35211-9 pubmed
    ..Altogether our results indicate that virus-associated C-PKA regulates HIV-1 infectivity, possibly by catalyzing phosphorylation of the viral CAp24gag protein. ..
  4. Zhong H, Voll R, Ghosh S. Phosphorylation of NF-kappa B p65 by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300. Mol Cell. 1998;1:661-71 pubmed
    ..Therefore, PKA regulates the transcriptional activity of NF-kappa B by modulating its interaction with CBP/p300. ..
  5. Anthonsen M, Ronnstrand L, Wernstedt C, Degerman E, Holm C. Identification of novel phosphorylation sites in hormone-sensitive lipase that are phosphorylated in response to isoproterenol and govern activation properties in vitro. J Biol Chem. 1998;273:215-21 pubmed
  6. Taurin S, Sandbo N, Qin Y, Browning D, Dulin N. Phosphorylation of beta-catenin by cyclic AMP-dependent protein kinase. J Biol Chem. 2006;281:9971-6 pubmed
    ..In conclusion, this study identifies a novel, noncanonical mechanism of modulation of beta-catenin signaling through direct phosphorylation of beta-catenin by PKA, promoting its interaction with CREB-binding protein. ..
  7. Tanji C, Yamamoto H, Yorioka N, Kohno N, Kikuchi K, Kikuchi A. A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta. J Biol Chem. 2002;277:36955-61 pubmed
    ..These results suggest that PKA and PP1 regulate the activity of GSK-3beta efficiently by forming a complex with AKAP220. ..
  8. Muroi Y, Sakurai T, Hanashi A, Kubota K, Nagaoka K, Imakawa K. CD9 regulates transcription factor GCM1 and ERVWE1 expression through the cAMP/protein kinase A signaling pathway. Reproduction. 2009;138:945-51 pubmed publisher
    ..Moreover, CD9-induced increase in ERVWE1 and GCM1 expressions were inhibited by Rp-cAMPS. These results suggest that CD9 increases GCM1 expression via the cAMP/PKA signaling pathway, resulting in the increase in ERVWE1 expression. ..
  9. Shi J, Qian W, Yin X, Iqbal K, Grundke Iqbal I, Gu X, et al. Cyclic AMP-dependent protein kinase regulates the alternative splicing of tau exon 10: a mechanism involved in tau pathology of Alzheimer disease. J Biol Chem. 2011;286:14639-48 pubmed publisher
    ..These findings suggest that a down-regulation of PKA dysregulates the alternative splicing of tau exon 10 and contributes to neurofibrillary degeneration in AD by causing an imbalance in 3R-tau and 4R-tau expression. ..
  10. Beuschlein F, Fassnacht M, Assié G, Calebiro D, Stratakis C, Osswald A, et al. Constitutive activation of PKA catalytic subunit in adrenal Cushing's syndrome. N Engl J Med. 2014;370:1019-28 pubmed publisher
    ..Exome sequencing revealed somatic mutations in PRKACA, which encodes the catalytic subunit of cyclic AMP-dependent protein kinase (protein kinase A [PKA]), in 8 of 10 ..
  11. Matsuoka Y, Hughes C, Bennett V. Adducin regulation. Definition of the calmodulin-binding domain and sites of phosphorylation by protein kinases A and C. J Biol Chem. 1996;271:25157-66 pubmed
    ..These findings suggest a complex reciprocal relationship between regulation of adducin function by calmodulin binding and phosphorylation by PKA and PKC. ..
  12. Pasqualucci L, Kitaura Y, Gu H, Dalla Favera R. PKA-mediated phosphorylation regulates the function of activation-induced deaminase (AID) in B cells. Proc Natl Acad Sci U S A. 2006;103:395-400 pubmed
    ..These findings implicate PKA in the regulation of AID function and suggest that the control of T cell-dependent immune responses may be modulated, via AID, by signals that activate PKA. ..
  13. Saxena M, Williams S, Tasken K, Mustelin T. Crosstalk between cAMP-dependent kinase and MAP kinase through a protein tyrosine phosphatase. Nat Cell Biol. 1999;1:305-11 pubmed
    ..We conclude that HePTP not only controls the activity of MAP kinases, but also mediates crosstalk between the cAMP system and the MAP-kinase cascade. ..
  14. Soloaga A, Thomson S, Wiggin G, Rampersaud N, Dyson M, Hazzalin C, et al. MSK2 and MSK1 mediate the mitogen- and stress-induced phosphorylation of histone H3 and HMG-14. EMBO J. 2003;22:2788-97 pubmed
    ..We conclude that MSKs are the major kinases for histone H3 and HMG-14 in response to mitogenic and stress stimuli in fibroblasts. ..
  15. Rashid G, Bernheim J, Green J, Benchetrit S. Parathyroid hormone stimulates the endothelial nitric oxide synthase through protein kinase A and C pathways. Nephrol Dial Transplant. 2007;22:2831-7 pubmed
    ..PTH induces an increased activity of the eNOS system; probably both PKA and PKC pathways are involved in this activation. Such data may explain the vasodilation observed after acute treatment with PTH. ..
  16. Vermeulen L, De Wilde G, Van Damme P, Vanden Berghe W, Haegeman G. Transcriptional activation of the NF-kappaB p65 subunit by mitogen- and stress-activated protein kinase-1 (MSK1). EMBO J. 2003;22:1313-24 pubmed
    ..This effect represents, together with phosphorylation of nucleosome components such as histone H3, an essential step leading to selective transcriptional activation of NF-kappaB-dependent gene expression. ..
  17. Meoli E, Bossis I, Cazabat L, Mavrakis M, Horvath A, Stergiopoulos S, et al. Protein kinase A effects of an expressed PRKAR1A mutation associated with aggressive tumors. Cancer Res. 2008;68:3133-41 pubmed publisher
  18. Sette C, Conti M. Phosphorylation and activation of a cAMP-specific phosphodiesterase by the cAMP-dependent protein kinase. Involvement of serine 54 in the enzyme activation. J Biol Chem. 1996;271:16526-34 pubmed
    ..These findings support the hypothesis that the PDE4D3 variant contains a regulatory domain target for phosphorylation at the amino terminus of the protein and that Ser54 in this domain plays a crucial role in activation. ..
  19. Cardone R, Bagorda A, Bellizzi A, Busco G, Guerra L, Paradiso A, et al. Protein kinase A gating of a pseudopodial-located RhoA/ROCK/p38/NHE1 signal module regulates invasion in breast cancer cell lines. Mol Biol Cell. 2005;16:3117-27 pubmed
  20. Sastri M, Barraclough D, Carmichael P, Taylor S. A-kinase-interacting protein localizes protein kinase A in the nucleus. Proc Natl Acad Sci U S A. 2005;102:349-54 pubmed
    ..Thus, AKIP1 describes a PKA-interacting protein that can contribute to localization by a mechanism that is distinct from A-kinase anchoring proteins that interact with the regulatory subunits. ..
  21. Ellerbroek S, Wennerberg K, Burridge K. Serine phosphorylation negatively regulates RhoA in vivo. J Biol Chem. 2003;278:19023-31 pubmed
  22. Reinton N, Orstavik S, Haugen T, Jahnsen T, Tasken K, Skalhegg B. A novel isoform of human cyclic 3',5'-adenosine monophosphate-dependent protein kinase, c alpha-s, localizes to sperm midpiece. Biol Reprod. 2000;63:607-11 pubmed
    ..This novel C alpha-s splice variant appeared to have an independent anchor in the human sperm midpiece as it could not be completely solubilized even in the presence of both detergent and cAMP. ..
  23. Zhong H, SuYang H, Erdjument Bromage H, Tempst P, Ghosh S. The transcriptional activity of NF-kappaB is regulated by the IkappaB-associated PKAc subunit through a cyclic AMP-independent mechanism. Cell. 1997;89:413-24 pubmed
    ..Therefore, this pathway represents a novel mechanism for the cAMP-independent activation of PKA and the regulation of NF-kappaB activity. ..
  24. Gibson R, Taylor S. Dissecting the cooperative reassociation of the regulatory and catalytic subunits of cAMP-dependent protein kinase. Role of Trp-196 in the catalytic subunit. J Biol Chem. 1997;272:31998-2005 pubmed
    ..One of these mutants, rR(R333K), having a defect in cAMP binding site B formed a stable complex with rC(W196R) in the absence of cAMP. However, unlike wild-type holoenzyme, this complex was active. ..
  25. Taylor S, Buechler J, Yonemoto W. cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes. Annu Rev Biochem. 1990;59:971-1005 pubmed
  26. Suko J, Maurer Fogy I, Plank B, Bertel O, Wyskovsky W, Hohenegger M, et al. Phosphorylation of serine 2843 in ryanodine receptor-calcium release channel of skeletal muscle by cAMP-, cGMP- and CaM-dependent protein kinase. Biochim Biophys Acta. 1993;1175:193-206 pubmed
  27. de Groot R, den Hertog J, Vandenheede J, Goris J, Sassone Corsi P. Multiple and cooperative phosphorylation events regulate the CREM activator function. EMBO J. 1993;12:3903-11 pubmed
    ..These results show that the activity of CREM tau is regulated by multiple phosphorylation events, suggesting that CREM could be considered as a nuclear effector where signalling pathways may converge and/or cross-talk. ..
  28. Chheda M, Ashery U, Thakur P, Rettig J, Sheng Z. Phosphorylation of Snapin by PKA modulates its interaction with the SNARE complex. Nat Cell Biol. 2001;3:331-8 pubmed
    ..Our results indicate that Snapin may be a PKA target for modulating transmitter release through the cAMP-dependent signal-transduction pathway. ..
  29. Chung S, Furihata M, Tamura K, Uemura M, Daigo Y, Nasu Y, et al. Overexpressing PKIB in prostate cancer promotes its aggressiveness by linking between PKA and Akt pathways. Oncogene. 2009;28:2849-59 pubmed publisher
    ..We found the direct interaction between PKIB and cAMP-dependent protein kinase A catalytic subunit (PKA-C), and showed that knockdown of PKIB in PC cells diminished the nuclear translocation of PKA-C...
  30. Janknecht R. Regulation of the ER81 transcription factor and its coactivators by mitogen- and stress-activated protein kinase 1 (MSK1). Oncogene. 2003;22:746-55 pubmed
    ..Thus, MSK1 may regulate ER81-dependent transcription via direct phosphorylation of ER81 as well as via stimulation of CBP/p300, which might be important for ER81's normal function and during mammary tumor formation. ..
  31. Janknecht R. Cell type-specific inhibition of the ETS transcription factor ER81 by mitogen-activated protein kinase-activated protein kinase 2. J Biol Chem. 2001;276:41856-61 pubmed
    ..Altogether, MK2 may regulate ER81 transcriptional activity in a cell type-specific manner and thereby modulate various physiological processes beyond stress responses. ..
  32. Baude E, Dignam S, Olsen S, Reimann E, Uhler M. Glutamic acid 203 of the cAMP-dependent protein kinase catalytic subunit participates in the inhibition by two isoforms of the protein kinase inhibitor. J Biol Chem. 1994;269:2316-23 pubmed
    ..Preliminary studies of the expression of these C alpha mutants in COS cells gave similar results. These results suggest that the C alpha E203 mutants may be useful in assessing the role of PKI in vivo. ..
  33. Nakajima Y, Okamura T, Horiguchi K, Gohko T, Miyamoto T, Satoh T, et al. GNAS mutations in adrenal aldosterone-producing adenomas. Endocr J. 2016;63:199-204 pubmed publisher
    ..have also recently been reported in adrenal cortisol-producing adenomas (CPAs), in addition to those in the PRKACA gene. However, mutations have not yet been examined in aldosterone-producing adenomas (APAs)...
  34. Schneider A, Biernat J, von Bergen M, Mandelkow E, Mandelkow E. Phosphorylation that detaches tau protein from microtubules (Ser262, Ser214) also protects it against aggregation into Alzheimer paired helical filaments. Biochemistry. 1999;38:3549-58 pubmed
    ..This implies that the hyperphosphorylation of tau in Alzheimer's disease is not directly responsible for the pathological aggregation into PHFs; on the contrary, phosphorylation protects tau against aggregation. ..
  35. Antonsson B, Lutjens R, Di Paolo G, Kassel D, Allet B, Bernard A, et al. Purification, characterization, and in vitro phosphorylation of the neuron-specific membrane-associated protein SCG10. Protein Expr Purif. 1997;9:363-71 pubmed
    ..Our data suggest that SCG10 is a phosphoprotein which might be involved in signal transduction in neurons. ..
  36. Zidovetzki R, Wang J, Chen P, Jeyaseelan R, Hofman F. Human immunodeficiency virus Tat protein induces interleukin 6 mRNA expression in human brain endothelial cells via protein kinase C- and cAMP-dependent protein kinase pathways. AIDS Res Hum Retroviruses. 1998;14:825-33 pubmed
    ..Both the Tat-induced increase in intracellular cAMP and IL-6 mRNA levels in CNS-ECs may play a role in altering the blood-brain barrier and thereby inducing pathology often observed in AIDS dementia. ..
  37. Parnell E, Koschinski A, Zaccolo M, Cameron R, Baillie G, Baillie G, et al. Phosphorylation of ezrin on Thr567 is required for the synergistic activation of cell spreading by EPAC1 and protein kinase A in HEK293T cells. Biochim Biophys Acta. 2015;1853:1749-58 pubmed publisher
  38. Katayama H, Wang J, Treekitkarnmongkol W, Kawai H, Sasai K, Zhang H, et al. Aurora kinase-A inactivates DNA damage-induced apoptosis and spindle assembly checkpoint response functions of p73. Cancer Cell. 2012;21:196-211 pubmed publisher
    ..Elevated cytoplasmic p73 in Aurora-A overexpressing primary human tumors corroborates the experimental findings. ..
  39. Leonoudakis D, Mailliard W, Wingerd K, Clegg D, Vandenberg C. Inward rectifier potassium channel Kir2.2 is associated with synapse-associated protein SAP97. J Cell Sci. 2001;114:987-98 pubmed
    ..These results identify a direct association of Kir2.1, Kir2.2 and Kir2.3 with the MAGUK family member SAP97 that may form part of a macromolecular signaling complex in many different tissues. ..
  40. Chen D, Pace P, Coombes R, Ali S. Phosphorylation of human estrogen receptor alpha by protein kinase A regulates dimerization. Mol Cell Biol. 1999;19:1002-15 pubmed
    ..Thus, phosphorylation of ERalpha in the DNA binding domain provides a mechanism by which dimerization and thereby DNA binding by the estrogen receptor is regulated. ..
  41. Chowdhury S, Howell G, Rajput A, Teggart C, Brattain L, Weber H, et al. Identification of a novel TGF?/PKA signaling transduceome in mediating control of cell survival and metastasis in colon cancer. PLoS ONE. 2011;6:e19335 pubmed publisher
    ..Development of effective treatments for metastatic disease is a pressing need since metastases are the major cause of death in solid tumors. ..
  42. Lee J, Chen H, Pullikotil P, Quon M. Protein kinase A-alpha directly phosphorylates FoxO1 in vascular endothelial cells to regulate expression of vascular cellular adhesion molecule-1 mRNA. J Biol Chem. 2011;286:6423-32 pubmed publisher
    ..We conclude that FoxO1 is a novel physiological substrate for PKA-? in vascular endothelial cells. ..
  43. Owczarek S, Bang M, Berezin V. Neurexin-Neuroligin Synaptic Complex Regulates Schizophrenia-Related DISC1/Kal-7/Rac1 "Signalosome". Neural Plast. 2015;2015:167308 pubmed publisher
    ..Our results indicate that the NX/NL synaptic complex is intrinsically involved in the regulation of DISC1 function, thus contributing to a better understanding of the pathology of schizophrenia. ..
  44. Sapkota G, Kieloch A, Lizcano J, Lain S, Arthur J, Williams M, et al. Phosphorylation of the protein kinase mutated in Peutz-Jeghers cancer syndrome, LKB1/STK11, at Ser431 by p90(RSK) and cAMP-dependent protein kinase, but not its farnesylation at Cys(433), is essential for LKB1 to suppress cell vrowth. J Biol Chem. 2001;276:19469-82 pubmed
    ..In contrast, a mutant of LKB1 that cannot be prenylated was still able to suppress the growth of cells. ..
  45. Gao X, Patel T. Regulation of protein kinase A activity by p90 ribosomal S6 kinase 1. J Biol Chem. 2009;284:33070-8 pubmed publisher
    ..These findings demonstrate that interactions between endogenous RSK1 and PKAc in intact cells regulate the ability of cAMP to activate PKA and identify a novel mechanism by which PKA activity is regulated by the Erk1/2 pathway. ..
  46. Tzitzivacos D, Tiemessen C, Stevens W, Papathanasopoulos M. Viral genetic determinants of nonprogressive HIV type 1 subtype C infection in antiretroviral drug-naive children. AIDS Res Hum Retroviruses. 2009;25:1141-8 pubmed publisher
    ..For example, LT46 Nef is unable to bind AP-1 and AP-2 and therefore is inactive on CD4 endocytosis. The biological relevance of these findings requires further investigation. ..
  47. Feng H, Li Y, Yin Y, Zhang W, Hou Y, Zhang L, et al. Protein kinase A-dependent phosphorylation of Dock180 at serine residue 1250 is important for glioma growth and invasion stimulated by platelet derived-growth factor receptor α. Neuro Oncol. 2015;17:832-42 pubmed publisher
  48. Marx S, Reiken S, Hisamatsu Y, Jayaraman T, Burkhoff D, Rosemblit N, et al. PKA phosphorylation dissociates FKBP12.6 from the calcium release channel (ryanodine receptor): defective regulation in failing hearts. Cell. 2000;101:365-76 pubmed
    ..6, PKA, the protein phosphatases PP1 and PP2A, and an anchoring protein, mAKAP. In failing human hearts, RyR2 is PKA hyperphosphorylated, resulting in defective channel function due to increased sensitivity to Ca2+-induced activation. ..
  49. Baude E, Dignam S, Reimann E, Uhler M. Evidence for the importance of hydrophobic residues in the interactions between the cAMP-dependent protein kinase catalytic subunit and the protein kinase inhibitors. J Biol Chem. 1994;269:18128-33 pubmed
  50. Srivenugopal K, Mullapudi S, Shou J, Hazra T, Ali Osman F. Protein phosphorylation is a regulatory mechanism for O6-alkylguanine-DNA alkyltransferase in human brain tumor cells. Cancer Res. 2000;60:282-7 pubmed
    ..These data provide the first and conclusive evidence of AGT phosphorylation and suggest that reversible phosphorylation may control the activity of this therapeutically important DNA repair protein in human normal and cancer cells. ..
  51. Nin V, Escande C, Chini C, Giri S, Camacho Pereira J, Matalonga J, et al. Role of deleted in breast cancer 1 (DBC1) protein in SIRT1 deacetylase activation induced by protein kinase A and AMP-activated protein kinase. J Biol Chem. 2012;287:23489-501 pubmed publisher
    ..Our experiments provide new insight on the in vivo mechanism of SIRT1 regulation and a new avenue for the development of pharmacological SIRT1 activators targeted at the dissociation of the SIRT1-DBC1 complex. ..
  52. Alazami A, Awad S, Coskun S, Al Hassan S, Hijazi H, Abdulwahab F, et al. TLE6 mutation causes the earliest known human embryonic lethality. Genome Biol. 2015;16:240 pubmed publisher
    ..In this first report of a human defect in a member of the subcortical maternal subcritical maternal complex, we show that the TLE6 mutation is gender-specific and leads to the earliest known human embryonic lethality phenotype. ..
  53. Kim S, Cho Y, Kim H, Oh J, Ahn E, Ko H, et al. Antagonism of VEGF-A-induced increase in vascular permeability by an integrin ?3?1-Shp-1-cAMP/PKA pathway. Blood. 2012;120:4892-902 pubmed publisher
    ..Our results demonstrate the potential utility for TIMP-2 in cancer therapy through "normalization" of vascular permeability in addition to previously described antiangiogenic effects. ..
  54. Nanba K, Omata K, Tomlins S, Giordano T, Hammer G, Rainey W, et al. Double adrenocortical adenomas harboring independent KCNJ5 and PRKACA somatic mutations. Eur J Endocrinol. 2016;175:K1-6 pubmed publisher
    ..T148I/T149S, present in the same reads) and a PRKACA gene hotspot mutation (p.L206R) in the CYP11B2-positive and -negative tumors, respectively...
  55. Potter L, Hunter T. Identification and characterization of the phosphorylation sites of the guanylyl cyclase-linked natriuretic peptide receptors A and B. Methods. 1999;19:506-20 pubmed
    ..In this review, we give a detailed description of the phosphopeptide mapping techniques that were used to identify and characterize these sites and discuss the potential pitfalls that are associated with them. ..
  56. Saja K, Chatterjee U, Chatterjee B, Sudhakaran P. Activation dependent expression of MMPs in peripheral blood mononuclear cells involves protein kinase A. Mol Cell Biochem. 2007;296:185-92 pubmed
  57. Bignone P, King M, Pinder J, Baines A. Phosphorylation of a threonine unique to the short C-terminal isoform of betaII-spectrin links regulation of alpha-beta spectrin interaction to neuritogenesis. J Biol Chem. 2007;282:888-96 pubmed
    ..Furthermore, because both the T2159E mutant and the wild-type allow neuritogenesis, we conclude that the short C-terminal betaII-spectrin is phosphorylated during this process. ..
  58. Markoutsa S, Sürün D, Karas M, Hofmann B, Steinhilber D, Sorg B. Analysis of 5-lipoxygenase phosphorylation on molecular level by MALDI-MS. FEBS J. 2014;281:1931-47 pubmed
  59. Wang W, Zhang X, Zheng J, Yang J. High glucose stimulates adipogenic and inhibits osteogenic differentiation in MG-63 cells through cAMP/protein kinase A/extracellular signal-regulated kinase pathway. Mol Cell Biochem. 2010;338:115-22 pubmed publisher
  60. Yarrow J, Totsukawa G, Charras G, Mitchison T. Screening for cell migration inhibitors via automated microscopy reveals a Rho-kinase inhibitor. Chem Biol. 2005;12:385-95 pubmed
    ..We also profile the wound healing assay with a library of compounds with known bioactivities, revealing multiple pathways involved in the biology. ..
  61. Blanco Aparicio C, Torres J, Pulido R. A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase. J Cell Biol. 1999;147:1129-36 pubmed
    ..These findings support the existence of a novel mechanism by which PKA may regulate the activation and translocation to the nucleus of MAP kinases. ..
  62. Ronchi C, Peverelli E, Herterich S, Weigand I, Mantovani G, Schwarzmayr T, et al. Landscape of somatic mutations in sporadic GH-secreting pituitary adenomas. Eur J Endocrinol. 2016;174:363-72 pubmed publisher
    ..Recently, mutations affecting the USP8 and PRKACA gene have been reported in ACTH-secreting pituitary adenomas and cortisol-secreting adrenocortical adenomas ..
  63. Deming P, Campbell S, Stone J, Rivard R, Mercier A, Howe A. Anchoring of protein kinase A by ERM (ezrin-radixin-moesin) proteins is required for proper netrin signaling through DCC (deleted in colorectal cancer). J Biol Chem. 2015;290:5783-96 pubmed publisher
    ..These data support a model in which ERM-mediated anchoring of PKA activity to DCC is required for proper netrin/DCC-mediated signaling. ..
  64. Manna S, Babajan B, Raghavendra P, Raviprakash N, Sureshkumar C. Inhibiting TRAF2-mediated activation of NF-kappaB facilitates induction of AP-1. J Biol Chem. 2010;285:11617-27 pubmed publisher
    ..These results suggest novel approaches to design of P(3)-25 as an anti-cancer/inflammatory drug for therapy through regulation of the TRAF2 pathway. ..
  65. Gauthier E, Rahuel C, Wautier M, El Nemer W, Gane P, Wautier J, et al. Protein kinase A-dependent phosphorylation of Lutheran/basal cell adhesion molecule glycoprotein regulates cell adhesion to laminin alpha5. J Biol Chem. 2005;280:30055-62 pubmed
    ..It is postulated that modulation of the phosphorylation state of Lu gp might be a critical factor for the sickle red cells adhesiveness to laminin alpha5 in sickle cell disease...
  66. Burnatowska Hledin M, Zhao P, Capps B, Poel A, Parmelee K, Mungall C, et al. VACM-1, a cullin gene family member, regulates cellular signaling. Am J Physiol Cell Physiol. 2000;279:C266-73 pubmed
    ..Our data demonstrate that VACM-1, the first mammalian cullin protein to be characterized, is involved in the regulation of signaling...
  67. K ck K, Koenen A, Giese B, Fraunholz M, May K, Siegmund W, et al. Rapid modulation of the organic anion transporting polypeptide 2B1 (OATP2B1, SLCO2B1) function by protein kinase C-mediated internalization. J Biol Chem. 2010;285:11336-47 pubmed publisher
    ..Furthermore, internalization could be shown in an ex vivo placenta perfusion. Our findings represent a new, rapid mechanism in regulation of human OATPs...
  68. Qi Q, Rajala R, Anderson W, Jiang C, Rozwadowski K, Selvaraj G, et al. Molecular cloning, genomic organization, and biochemical characterization of myristoyl-CoA:protein N-myristoyltransferase from Arabidopsis thaliana. J Biol Chem. 2000;275:9673-83 pubmed
    ..The results presented in this study provide the first direct molecular evidence for plant protein N-myristoylation and a mechanistic basis for understanding the role of this protein modification in plants. ..
  69. Yu P, Yang Z, Jones J, Wang Z, Owens S, Mueller S, et al. D1 dopamine receptor signaling involves caveolin-2 in HEK-293 cells. Kidney Int. 2004;66:2167-80 pubmed
    ..The majority of hD1Rs are distributed in lipid rafts. Heterologously and endogenously expressed D1Rs in renal cells are associated with and regulated by caveolin-2. ..
  70. Bergelin N, Löf C, Balthasar S, Kalhori V, Törnquist K. S1P1 and VEGFR-2 form a signaling complex with extracellularly regulated kinase 1/2 and protein kinase C-alpha regulating ML-1 thyroid carcinoma cell migration. Endocrinology. 2010;151:2994-3005 pubmed publisher
    ..We hypothesize that VEGFR-2 forms a signaling complex with S1P(1), evoking bidirectional signaling regulating both ERK1/2 phosphorylation and haptotaxis of ML-1 cells. ..
  71. Goh G, Scholl U, Healy J, Choi M, Prasad M, Nelson Williams C, et al. Recurrent activating mutation in PRKACA in cortisol-producing adrenal tumors. Nat Genet. 2014;46:613-7 pubmed publisher
    ..Six others had somatic mutations in PRKACA (protein kinase A (PKA) catalytic subunit) resulting in a p.Leu206Arg substitution...
  72. Matsushita M, Nairn A. Inhibition of the Ca2+/calmodulin-dependent protein kinase I cascade by cAMP-dependent protein kinase. J Biol Chem. 1999;274:10086-93 pubmed
    ..The phosphorylation and inhibition of CaMKK by PKA is likely to be involved in modulating the balance between cAMP- and Ca2+-dependent signal transduction pathways. ..
  73. Lou L, Urbani J, Ribeiro Neto F, Altschuler D. cAMP inhibition of Akt is mediated by activated and phosphorylated Rap1b. J Biol Chem. 2002;277:32799-806 pubmed
  74. Swanson D, Zellmer E, Lewis E. The homeodomain protein Arix interacts synergistically with cyclic AMP to regulate expression of neurotransmitter biosynthetic genes. J Biol Chem. 1997;272:27382-92 pubmed
    ..These results demonstrate a synergistic interaction between a homeodomain protein and the cAMP signal transduction system and suggest that similar interactions may regulate the tissue-specific expression of neuroendocrine genes. ..
  75. Gupte R, Weng Y, Liu L, Lee M. The second subunit of the replication factor C complex (RFC40) and the regulatory subunit (RIalpha) of protein kinase A form a protein complex promoting cell survival. Cell Cycle. 2005;4:323-9 pubmed
    ..Interestingly, downregulation of endogenous RIalpha by 8-chloro cAMP, in MCF7 breast cancer cells led to reduction in the amount of RFC40-RIalpha complex, together with decrease in cell survival. ..
  76. Hepp R, Cabaniols J, Roche P. Differential phosphorylation of SNAP-25 in vivo by protein kinase C and protein kinase A. FEBS Lett. 2002;532:52-6 pubmed
    ..Finally, despite published data to the contrary, we demonstrate that stimulation of regulated exocytosis under physiological conditions is independent of a measurable increase in SNAP-25 phosphorylation in PC12 cells. ..
  77. Hosaka M, Hammer R, Sudhof T. A phospho-switch controls the dynamic association of synapsins with synaptic vesicles. Neuron. 1999;24:377-87 pubmed
    ..The dynamic association of synapsins with synaptic vesicles correlates with their role in activity-dependent plasticity. ..
  78. Azim A, Knoll J, Beggs A, Chishti A. Isoform cloning, actin binding, and chromosomal localization of human erythroid dematin, a member of the villin superfamily. J Biol Chem. 1995;270:17407-13 pubmed
    ..The dematin locus, 8p21.1, is distal to the known locus of human erythroid ankyrin (8p11.2) and may contribute to the etiology of hemolytic anemia in a subset of patients with severe hereditary spherocytosis. ..