Genomes and Genes
Gene Symbol: PRKACA
Description: protein kinase, cAMP-dependent, catalytic, alpha
Alias: PKACA, PPNAD4, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha, protein kinase A catalytic subunit
Publications206 found, 100 shown here
- 3-ketosteroid reductase activity and expression by fetal rat osteoblastsThomas L McCarthy
Department of Surgery, Section of Plastic Surgery, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
J Biol Chem 282:34003-12. 2007..AKR1C9 promoter activity was also enhanced by overexpression of protein kinase A catalytic subunit or transcription factor C/EBPdelta, and the effect of PGE2 was reduced by dominant negative C/..
- Melanocyte-lineage expression of Cre recombinase using Mitf regulatory elementsAzita Alizadeh
Departments of Genetics and Pediatrics, Stanford University School of Medicine, Stanford, CA, USA
Pigment Cell Melanoma Res 21:63-9. 2008..utility of the MitfCre transgene, we used a loxP-targeted allele of the protein kinase A alpha catalytic subunit (Prkaca), modified such that Cre-mediated recombination activates PKA signaling...
- Odorant receptors at the growth cone are coupled to localized cAMP and Ca2+ increasesMicol Maritan
Venetian Institute of Molecular Medicine, Via Orus 2, 35129 Padova, Italy
Proc Natl Acad Sci U S A 106:3537-42. 2009..that selective odor activation of the OR on the growth cone is followed by nuclear translocation of protein kinase A catalytic subunit. These results define the functional properties of the OR on the growth cone and suggest a potential ..
- Protein kinase A catalytic subunit interacts and phosphorylates members of trans-sialidase super-family in Trypanosoma cruziYi Bao
Department of Pathology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
Microbes Infect 12:716-26. 2010..The identified trans-sialidases have PKA consensus phosphorylation sites located near the endoplasmic reticulum retention motif in the N-terminal. These data support that PKA phosphorylates trans-sialidase super family members in vivo...
- The N terminus of adenovirus type 12 E1A inhibits major histocompatibility complex class I expression by preventing phosphorylation of NF-kappaB p65 Ser276 through direct bindingJunfang Jiao
Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Virol 84:7668-74. 2010..by the Ad12 E1A protein (E1A-12), which prevents NF-kappaB from becoming phosphorylated by the protein kinase A catalytic subunit (PKAc). In this study, we examined the interactions between E1A-12 and NF-kappaB...
- Transcriptional activation of the NF-kappaB p65 subunit by mitogen- and stress-activated protein kinase-1 (MSK1)Linda Vermeulen
Department of Molecular Biology, University of Gent VIB, K L Ledeganckstraat 35, B 9000 Gent, Belgium
EMBO J 22:1313-24. 2003..This effect represents, together with phosphorylation of nucleosome components such as histone H3, an essential step leading to selective transcriptional activation of NF-kappaB-dependent gene expression...
- Regulation of the ER81 transcription factor and its coactivators by mitogen- and stress-activated protein kinase 1 (MSK1)Ralf Janknecht
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
Oncogene 22:746-55. 2003..Thus, MSK1 may regulate ER81-dependent transcription via direct phosphorylation of ER81 as well as via stimulation of CBP/p300, which might be important for ER81's normal function and during mammary tumor formation...
- Phosphorylation of beta-catenin by cyclic AMP-dependent protein kinaseSebastien Taurin
Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, 5841 S Maryland Avenue, Chicago, IL 60637, USA
J Biol Chem 281:9971-6. 2006..In conclusion, this study identifies a novel, noncanonical mechanism of modulation of beta-catenin signaling through direct phosphorylation of beta-catenin by PKA, promoting its interaction with CREB-binding protein...
- Protein kinase A binds and activates heat shock factor 1Ayesha Murshid
Molecular and Cellular Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS ONE 5:e13830. 2010..As the mechanisms involved in the intracellular regulation of HSF1 in good health and its dysregulation in disease are still incomplete we are investigating the role of posttranslational modifications in such regulation...
- Inhibiting TRAF2-mediated activation of NF-kappaB facilitates induction of AP-1Sunil K Manna
Laboratory of Immunology, Centre for DNA Fingerprinting and Diagnostics, Nampally, Hyderabad 500 001, India
J Biol Chem 285:11617-27. 2010..These results suggest novel approaches to design of P(3)-25 as an anti-cancer/inflammatory drug for therapy through regulation of the TRAF2 pathway...
- Prostaglandin E2 inhibits specific lung fibroblast functions via selective actions of PKA and Epac-1Steven K Huang
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109 5642, USA
Am J Respir Cell Mol Biol 39:482-9. 2008..The selective use of PKA and Epac-1 pathways to inhibit distinct aspects of fibroblast activation illustrate the pleiotropic ability of PGE(2) to inhibit diverse fibroblast functions...
- Adducin regulation. Definition of the calmodulin-binding domain and sites of phosphorylation by protein kinases A and CY Matsuoka
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 271:25157-66. 1996..These findings suggest a complex reciprocal relationship between regulation of adducin function by calmodulin binding and phosphorylation by PKA and PKC...
- Constitutive activation of PKA catalytic subunit in adrenal Cushing's syndromeFelix Beuschlein
The authors affiliations are listed in the Appendix
N Engl J Med 370:1019-28. 2014..Corticotropin-independent Cushing's syndrome is caused by tumors or hyperplasia of the adrenal cortex. The molecular pathogenesis of cortisol-producing adrenal adenomas is not well understood...
- Phosphorylation and activation of a cAMP-specific phosphodiesterase by the cAMP-dependent protein kinase. Involvement of serine 54 in the enzyme activationC Sette
Division of Reproductive Biology, Department of Gynecology and Obstetrics, Stanford University Medical Center, Stanford, California 94305 5317, USA
J Biol Chem 271:16526-34. 1996..These findings support the hypothesis that the PDE4D3 variant contains a regulatory domain target for phosphorylation at the amino terminus of the protein and that Ser54 in this domain plays a crucial role in activation...
- Protein kinase A effects of an expressed PRKAR1A mutation associated with aggressive tumorsElise Meoli
Section on Endocrinology and Genetics, Program in Developmental Endocrinology and Genetics, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
Cancer Res 68:3133-41. 2008....
- Phosphorylation of the inositol 1,4,5-trisphosphate receptor by cyclic GMP-dependent protein kinaseP Komalavilas
Department of Pathology, University of Alabama, Birmingham 35294 0019
J Biol Chem 269:8701-7. 1994..Phosphorylation of IP3 receptor by cGMP kinase may regulate the function of IP3 receptor in vascular smooth muscle cells and contribute to the effect of cGMP to regulate intracellular calcium levels...
- Protein kinase A is central for forward transport of two-pore domain potassium channels K2P3.1 and K2P9.1Alexandra Mant
Division of Human Genetics, Centre for Human Development, Stem Cells and Regeneration, University of Southampton, Duthie Building, Mail Point 808, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, United Kingdom
J Biol Chem 286:14110-9. 2011..All of our findings support the conclusion that cAMP-dependent protein kinase is responsible for the phosphorylation of the terminal serine in both K2P3.1 and K2P9.1...
- Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259Amardeep S Dhillon
The Beatson Institute for Cancer Research, Bearsden, Glasgow G61 1BD, United Kingdom
Mol Cell Biol 22:3237-46. 2002..Using RafS259 mutants we also demonstrate that Raf-1 is the sole target for PKA inhibition of ERK and ERK-induced gene expression, and that Raf-1 inhibition is mediated mainly through serine 259 phosphorylation...
- The testis-specific Cα2 subunit of PKA is kinetically indistinguishable from the common Cα1 subunit of PKAMaike M Vetter
Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, PB 1046 Blindern, Oslo, Norway
BMC Biochem 12:40. 2011..the α-form of the catalytic (C) subunit of protein kinase A (PKA), designated Cα1 and Cα2, are encoded by the PRKACA gene. Whereas Cα1 is ubiquitous, Cα2 expression is restricted to the sperm cell...
- cAMP-dependent protein kinase phosphorylation of the acid-sensing ion channel-1 regulates its binding to the protein interacting with C-kinase-1A Soren Leonard
Department of Internal Medicine, Roy J and Lucille A Carver College of Medicine, Howard Hughes Medical Institute, University of Iowa, Iowa City, IA 52242, USA
Proc Natl Acad Sci U S A 100:2029-34. 2003..Thus, the ASIC1 C terminus contains two sites that influence its binding to PICK1. Regulation of this interaction by phosphorylation provides a mechanism to control the cellular localization of ASIC1...
- Expression, purification, and regulation of two isoforms of the inositol 1,4,5-trisphosphate 3-kinaseP J Woodring
Department of Pharmacology and Cancer Research Center, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
J Biol Chem 272:30447-54. 1997..Differential expression and regulation of the two inositol 1,4,5-trisphosphate 3-kinase isoforms provides multiple mechanisms for regulating the cytosolic level of inositol 1,4,5-trisphosphate in cells...
- Viral genetic determinants of nonprogressive HIV type 1 subtype C infection in antiretroviral drug-naive childrenD B Tzitzivacos
HIV Pathogenesis Research Laboratory, Department of Molecular Medicine and Haematology, University of the Witwatersrand Medical School, Parktown, Johannesburg 2193, South Africa
AIDS Res Hum Retroviruses 25:1141-8. 2009..For example, LT46 Nef is unable to bind AP-1 and AP-2 and therefore is inactive on CD4 endocytosis. The biological relevance of these findings requires further investigation...
- Parkinsonian neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and alpha-synuclein mutations promote Tau protein phosphorylation at Ser262 and destabilize microtubule cytoskeleton in vitroHamid Y Qureshi
Department of Neurology and Neurosurgery, McGill University, Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Quebec H3T 1E2, Canada
J Biol Chem 286:5055-68. 2011....
- Interleukin-3 and bryostatin-1 mediate hyperphosphorylation of BCL2 alpha in association with suppression of apoptosisW S May
Johns Hopkins Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231
J Biol Chem 269:26865-70. 1994..These findings strongly support a role for activated PKC in growth factor-induced Bc12 alpha phosphorylation as well as suppression of apoptosis...
- 14-3-3 proteins are required for maintenance of Raf-1 phosphorylation and kinase activityJ A Thorson
Center for Immunology and Department of Pathology, Washington University School of Medicine, St Louis, Missouri, USA
Mol Cell Biol 18:5229-38. 1998..This supports a paradigm in which the effects of phosphorylation on serine as well as tyrosine residues are mediated by inducible protein-protein interactions...
- Protein kinase A regulates caspase-9 activation by Apaf-1 downstream of cytochrome cMorag C Martin
Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Scotland, United Kingdom
J Biol Chem 280:15449-55. 2005..These data indicate that extracellular signals that elevate cyclic AMP and activate protein kinase A may suppress apoptosis by inhibiting apoptosome formation downstream of cytochrome c release from mitochondria...
- cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymesS S Taylor
Department of Chemistry, University of California, San Diego, La Jolla 92093
Annu Rev Biochem 59:971-1005. 1990....
- Scar/WAVE-1, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffoldR S Westphal
Howard Hughes Medical Institute, Vollum Institute, Portland, OR, USA
EMBO J 19:4589-600. 2000..Thus, we propose a previously unrecognized function for WAVE-1 as an actin-associated scaffolding protein that recruits PKA and Abl...
- The transcriptional activity of NF-kappaB is regulated by the IkappaB-associated PKAc subunit through a cyclic AMP-independent mechanismH Zhong
Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Cell 89:413-24. 1997..Therefore, this pathway represents a novel mechanism for the cAMP-independent activation of PKA and the regulation of NF-kappaB activity...
- Serine phosphorylation negatively regulates RhoA in vivoShawn M Ellerbroek
Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
J Biol Chem 278:19023-31. 2003....
- Phosphorylation of HIV Nef by cAMP-dependent protein kinasePei Lin Li
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
Virology 331:367-74. 2005..As this mutation played a major role in abrogating the Nef effect on HIV replication in unstimulated primary cells, we postulate that Nef phosphorylation by PKA is an important step in the viral life cycle in resting cells...
- Expression of a nonmyristylated variant of the catalytic subunit of protein kinase A during male germ-cell developmentJ L Desseyn
Department of Pharmacology, University of Washington, Seattle, WA 98195 7750, USA
Proc Natl Acad Sci U S A 97:6433-8. 2000..Because protein kinase A is thought to play a pivotal role in sperm motility and capacitation, the distinctive biochemical properties of the unmyristylated Calpha2 may be essential for fertility in the male...
- A-kinase-interacting protein localizes protein kinase A in the nucleusMira Sastri
The Howard Hughes Medical Institute and Departments of Chemistry and Biochemistry and Pharmacology, University of California at San Diego, La Jolla, CA 92093 0654, USA
Proc Natl Acad Sci U S A 102:349-54. 2005..Thus, AKIP1 describes a PKA-interacting protein that can contribute to localization by a mechanism that is distinct from A-kinase anchoring proteins that interact with the regulatory subunits...
- Cyclic AMP-dependent protein kinase regulates the alternative splicing of tau exon 10: a mechanism involved in tau pathology of Alzheimer diseaseJianhua Shi
Jiangsu Key Laboratory of Neuroregeneration, Medical School, Nantong University, Nantong, Jiangsu 226001, China
J Biol Chem 286:14639-48. 2011..These findings suggest that a down-regulation of PKA dysregulates the alternative splicing of tau exon 10 and contributes to neurofibrillary degeneration in AD by causing an imbalance in 3R-tau and 4R-tau expression...
- A novel isoform of human cyclic 3',5'-adenosine monophosphate-dependent protein kinase, c alpha-s, localizes to sperm midpieceN Reinton
Institute of Medical Biochemistry, University of Oslo, N 0317 Oslo, Norway
Biol Reprod 63:607-11. 2000..This novel C alpha-s splice variant appeared to have an independent anchor in the human sperm midpiece as it could not be completely solubilized even in the presence of both detergent and cAMP...
- Crosstalk between cAMP-dependent kinase and MAP kinase through a protein tyrosine phosphataseM Saxena
Laboratory of Signal Transduction, Sidney Kimmel Cancer Center, San Diego, California 92121, USA
Nat Cell Biol 1:305-11. 1999..We conclude that HePTP not only controls the activity of MAP kinases, but also mediates crosstalk between the cAMP system and the MAP-kinase cascade...
- Identification of novel phosphorylation sites in hormone-sensitive lipase that are phosphorylated in response to isoproterenol and govern activation properties in vitroM W Anthonsen
Department of Cell and Molecular Biology, Section for Molecular Signaling, Lund University, S 221 00 Lund, Sweden
J Biol Chem 273:215-21. 1998....
- Involvement of the catalytic subunit of protein kinase A and of HA95 in pre-mRNA splicingAnne Katrine Kvissel
Department of Nutrition, University of Oslo, Oslo, Norway
Exp Cell Res 313:2795-809. 2007..Our findings demonstrate that the nuclear PKA C subunit co-locates with HA95 in SFCs and regulates pre-mRNA splicing, possibly through a cAMP-independent mechanism...
- VACM-1, a cullin gene family member, regulates cellular signalingM Burnatowska-Hledin
Departments of Biology and Chemistry, Peale Science Center, Hope College, Holland, Michigan 49422 9000, USA
Am J Physiol Cell Physiol 279:C266-73. 2000..Our data demonstrate that VACM-1, the first mammalian cullin protein to be characterized, is involved in the regulation of signaling...
- Cyclic AMP blocks cell growth through Raf-1-dependent and Raf-1-independent mechanismsNicolas Dumaz
Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, London SW3 6JB, United Kingdom
Mol Cell Biol 22:3717-28. 2002..Thus, although Raf-1 appears to be an important cAMP target, other pathways are also targeted by cAMP, providing alternative mechanisms that lead to suppression of cell growth...
- Phosphorylation of inositol 1,4,5-trisphosphate receptors in parotid acinar cells. A mechanism for the synergistic effects of cAMP on Ca2+ signalingJason I E Bruce
Department of Pharmacology and Physiology, School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, New York 14642, USA
J Biol Chem 277:1340-8. 2002..In addition, this report supports the emerging consensus that phosphorylation at the level of the Ca(2+) release machinery is a broadly important mechanism by which cells can regulate Ca(2+)-mediated processes...
- Regulation of the CDK-related protein kinase PCTAIRE-1 and its possible role in neurite outgrowth in Neuro-2A cellsRalph Graeser
ICRF Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
J Cell Sci 115:3479-90. 2002....
- Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 VifKotaro Shirakawa
Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaracho, Sakyo ku, Kyoto 606 8507, Japan
Nat Struct Mol Biol 15:1184-91. 2008..These data imply that PKA-mediated phosphorylation of A3G can regulate the interaction between A3G and Vif...
- Effect of phosphorylation on activities of Rap1A to interact with Raf-1 and to suppress Ras-dependent Raf-1 activationC D Hu
Department of Physiology II, Kobe University School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
J Biol Chem 274:48-51. 1999....
- Properties and phosphorylation sites of baculovirus-expressed nuclear inhibitor of protein phosphatase-1 (NIPP-1)V Vulsteke
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
J Biol Chem 272:32972-8. 1997....
- Mitotic phosphorylation of the Oct-1 homeodomain and regulation of Oct-1 DNA binding activityN Segil
Laboratory of Molecular Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021
Science 254:1814-6. 1991..The inhibition of Oct-1 DNA binding during mitosis suggests a mechanism by which the general inhibition of transcription during mitosis might occur...
- Two distinct caveolin-1 domains mediate the functional interaction of caveolin-1 with protein kinase AB Razani
Department of Molecular Pharmacology and The Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
Am J Physiol Cell Physiol 281:C1241-50. 2001..e., the mutant acts in a dominant-negative fashion. Thus, this dominant-negative caveolin-1 mutant will be extremely valuable for assessing the functional role of endogenous caveolin-1 in regulating a variety of other signaling cascades...
- HIV-1 gp120 induces anergy in naive T lymphocytes through CD4-independent protein kinase-A-mediated signalingAnna Maria Masci
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Universita di Napoli Federico II, 5 Via S Pansini, I 80131 Naples, Italy
J Leukoc Biol 74:1117-24. 2003..These findings reveal a novel mechanism by which HIVenv may modulate the processes of clonal expansion, homeostatic proliferation, and terminal differentiation of the naive T lymphocyte subset...
- Stimulation of GCMa transcriptional activity by cyclic AMP/protein kinase A signaling is attributed to CBP-mediated acetylation of GCMaChing Wen Chang
Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 115, Taiwan
Mol Cell Biol 25:8401-14. 2005..Our results reveal a novel regulation of GCMa activity by cAMP-dependent protein acetylation and provide a molecular mechanism by which cAMP signaling regulates trophoblastic fusion...
- Proteasome function is regulated by cyclic AMP-dependent protein kinase through phosphorylation of Rpt6Fengxue Zhang
Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, University of Alabama, Birmingham, Alabama 35294, USA
J Biol Chem 282:22460-71. 2007..Thus, hormones using the PKA system can also regulate proteasomes often in concert with glucose metabolism. This finding might lead to novel strategies for the treatment of proteasome-related diseases...
- CD9 regulates transcription factor GCM1 and ERVWE1 expression through the cAMP/protein kinase A signaling pathwayYoshikage Muroi
Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, 113 8657 Tokyo, Japan
Reproduction 138:945-51. 2009..Moreover, CD9-induced increase in ERVWE1 and GCM1 expressions were inhibited by Rp-cAMPS. These results suggest that CD9 increases GCM1 expression via the cAMP/PKA signaling pathway, resulting in the increase in ERVWE1 expression...
- Protein kinase A phosphorylation activates Vpr-induced cell cycle arrest during human immunodeficiency virus type 1 infectionR Anthony Barnitz
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 10 Center Dr, Bethesda, MD 20892 1892, USA
J Virol 84:6410-24. 2010..Inhibition of PKA activity during HIV-1 infection abrogates Vpr cell cycle arrest. These findings provide new insight into the signaling event that activates Vpr cell cycle arrest, ultimately leading to the death of infected T cells...
- Parathyroid hormone stimulates the endothelial nitric oxide synthase through protein kinase A and C pathwaysGloria Rashid
Department of Nephrology and Hypertension, Meir Medical Center, Tchernichovsky 59, Kfar Saba 44281, Israel
Nephrol Dial Transplant 22:2831-7. 2007....
- Dissecting the cooperative reassociation of the regulatory and catalytic subunits of cAMP-dependent protein kinase. Role of Trp-196 in the catalytic subunitR M Gibson
Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093 0654, USA
J Biol Chem 272:31998-2005. 1997..One of these mutants, rR(R333K), having a defect in cAMP binding site B formed a stable complex with rC(W196R) in the absence of cAMP. However, unlike wild-type holoenzyme, this complex was active...
- Protein kinase A gating of a pseudopodial-located RhoA/ROCK/p38/NHE1 signal module regulates invasion in breast cancer cell linesRosa A Cardone
Department of General and Environmental Physiology, University of Bari, 70126 Bari, Italy
Mol Biol Cell 16:3117-27. 2005....
- Active cAMP-dependent protein kinase incorporated within highly purified HIV-1 particles is required for viral infectivity and interacts with viral capsid proteinChristine Cartier
Laboratoire Infections Retrovirales et Signalisation Cellulaire, Centre National de la Recherche Scientifique, UMR 5121 UM1, Institut de Biologie, CS 89508, 34960 Montpellier Cedex 2, France
J Biol Chem 278:35211-9. 2003..Altogether our results indicate that virus-associated C-PKA regulates HIV-1 infectivity, possibly by catalyzing phosphorylation of the viral CAp24gag protein...
- D-myo-inositol 1,4,5-trisphosphate 3-kinase A is activated by receptor activation through a calcium:calmodulin-dependent protein kinase II phosphorylation mechanismD Communi
Institute of Interdisciplinary Research, Free University of Brussels, Belgium
EMBO J 16:1943-52. 1997..In this study, direct evidence is provided for a novel regulation mechanism for Ins(1,4,5)P3 3-kinase (isoform A) in vitro and in intact cells...
- Control of rat GluR6 glutamate receptor open probability by protein kinase A and calcineurinS F Traynelis
Department of Pharmacology, Rollins Research Center, Emory University, Atlanta, GA 30322 3090, USA
J Physiol 503:513-31. 1997..0) and calcineurin (Po,peak near 0.5)...
- Apolipoprotein A-I activates cellular cAMP signaling through the ABCA1 transporterBassam Haidar
Cardiovascular Genetics Laboratory, Division of Cardiology, McGill University Health Centre Royal Victoria Hospital, Montreal, Quebec H3A 1A1, Canada
J Biol Chem 279:9963-9. 2004..In contrast, the protein kinase A catalytic subunit was able to phosphorylate ABCA1 similarly from mutant and normal cell lines in vitro...
- Protein kinase catalytic subunit (PKAcat) from bovine lens: purification, characterization and phosphorylation of lens crystallinsBhaswati Samanta
Department of Chemistry, Bose Institute, 93 1, A P C Road, Kolkata 700009, India
Mol Cell Biochem 304:155-65. 2007The purification and functional characterization of protein kinase A catalytic subunit (PKAcat) from bovine lens cytosol has been described...
- Functional characterization of two distinct Mg(2+) extrusion mechanisms in cardiac sarcolemmal vesiclesChristie Cefaratti
Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4970, USA
Mol Cell Biochem 303:63-72. 2007..6) Na(+)-dependent Mg(2+) transport could be restored by stimulating vesicles loaded with protein kinase A catalytic subunit and ATP with membrane-permeant cyclic-AMP analog; (7) extra-vesicular Mg(2+) could be accumulated in ..
- Proteasome-driven turnover of tryptophan hydroxylase is triggered by phosphorylation in RBL2H3 cells, a serotonin producing mast cell lineYoshiko Iida
Department of Bioscience, and Biotechnology Research Center, Teikyo University of Science and Technology, Yamanashi, Japan
Eur J Biochem 269:4780-8. 2002..These results indicated that the phosphorylation of TPH was a prerequisite for proteasome-driven TPH degradation...
- Molecular cloning and expression of the catalytic subunit of protein kinase A from Trypanosoma cruziHuan Huang
Department of Pathology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
Int J Parasitol 32:1107-15. 2002..protein kinases was utilised to amplify regions corresponding to the parasite homologue of the protein kinase A catalytic subunit. This putative protein kinase A fragment was used to isolate the entire gene from T...
- Hemocytes are essential for wing maturation in Drosophila melanogasterJ A Kiger
Molecular and Cellular Biology, University of California, 1 Shields Avenue, Davis, CA 95616, USA
Proc Natl Acad Sci U S A 98:10190-5. 2001..Second, ectopic expression of protein kinase A catalytic subunit (PKAc) using certain GAL4 enhancer detector strains mimics the batone wing phenotype and also ..
- G protein mutations in pituitary tumors: a study on Turkish patientsBeki Kan
Department of Biophysics, Marmara University School of Medicine, Istanbul, Turkey
Pituitary 6:75-80. 2003..in pituitary tumors from Turkish patients and to investigate the possibility of mutations of protein kinase A catalytic subunit (PKAC) that activates the downstream effectors of adenylyl cyclase...
- Paired-like homeodomain proteins Phox2a/Arix and Phox2b/NBPhox have similar genetic organization and independently regulate dopamine beta-hydroxylase gene transcriptionM Adachi
Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, Portland 97201, USA
DNA Cell Biol 19:539-54. 2000..from the rat DBH promoter, and transcription is synergistically increased in the presence of the protein kinase A catalytic subunit (PKA) plus either Arix or NBPhox...
- The use of phosphate-affinity SDS-PAGE to measure the cardiac troponin I phosphorylation site distribution in human heart muscleAndrew E Messer
National Heart and Lung Institute, Imperial College London, London, UK
Proteomics Clin Appl 3:1371-82. 2009..To test the method we phosphorylated pure cTnI with protein kinase A catalytic subunit and observed up to six bands corresponding to 0, 1P, 2P, 3P, 4P and 5P phospho-species...
- Gene and microRNA expression in p53-deficient day 8.5 mouse embryosHiromi Hosako
Department of Veterinary Physiology and Pharmacology, Texas A and M University, 434 VMR Building, College Station, TX 77843, USA
Birth Defects Res A Clin Mol Teratol 85:546-55. 2009..Here we attempt to identify genes and microRNAs (miRNAs) modified by deletion of p53 in day 8.5 mouse embryos...
- Mucor rouxii Rho1 protein; characterization and possible role in polarized growthSilvia Argimon
Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon 2, Piso 4, 1428, Buenos Aires, Argentina
Antonie Van Leeuwenhoek 91:237-51. 2007..The peptide IRRNSQKFV, containing Ser135 proved to be a good substrate for M. rouxii protein kinase A catalytic subunit. The over-expressed Rho1 fully complements a Saccharomyces cerevisiae null mutant...
- Properties of the cAMP-activated C1- current in choroid plexus epithelial cells isolated from the ratJ D Kibble
Cell Physiology Group, School of Biological Sciences, University of Manchester, UK
J Physiol 496:69-80. 1996..2. In patch clamp experiments, inclusion of 0.25 mM cAMP and 375 protein kinase A catalytic subunit (PKA) in the electrode solution caused activation of an inwardly rectifying current (21/23 cells)...
- A conserved deamidation site at Asn 2 in the catalytic subunit of mammalian cAMP-dependent protein kinase detected by capillary LC-MS and tandem mass spectrometryP T Jedrzejewski
Department of Central Spectroscopy, German Cancer Research Center, Heidelberg
Protein Sci 7:457-69. 1998..This may be especially significant in view of the recent knowledge that negative charges close to myristic acid in some proteins contribute to regulating their cellular localization...
- Protein kinase A catalytic subunit alters cardiac mitochondrial redox state and membrane potential via the formation of reactive oxygen speciesShiro Nagasaka
Division of Cardiology, Internal Medicine III, Hamamatsu University School of Medicine, Hamamatsu, Japan
Circ J 71:429-36. 2007..The identification of protein kinase A (PKA) anchoring proteins on mitochondria implies a direct effect of PKA on mitochondrial function. However, little is known about the relationship between PKA and mitochondrial metabolism...
- Ca2+/Calmodulin-dependent protein kinase II phosphorylation of ryanodine receptor does affect calcium sparks in mouse ventricular myocytesTao Guo
Department of Physiology and Cardiovascular Institute, Loyola University Chicago, Stritch School of Medicine, Maywood, IL 60153, USA
Circ Res 99:398-406. 2006..This effect was abolished by CaMKII inhibitor peptides. In contrast, protein kinase A catalytic subunit also enhanced Ca2+ spark frequency in WT, but had no effect in PLB-KO...
- Activation of cAMP-dependent signaling pathway induces mouse organic anion transporting polypeptide 2 expressionChuan Chen
Department of Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
Mol Pharmacol 71:1159-64. 2007..promoter containing this CRE site was induced in cells cotransfected with a plasmid encoding the protein kinase A catalytic subunit. Cotransfection of cells with a plasmid encoding the dominant-negative CRE binding protein (CREB) ..
- Sperm-specific protein kinase A catalytic subunit Calpha2 orchestrates cAMP signaling for male fertilityMichael A Nolan
Department of Pharmacology, University of Washington, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 101:13483-8. 2004..These results define several crucial roles of PKA in sperm cell biology, bringing together both known and unique PKA-mediated events that are necessary for male fertility...
- Changes in intracellular distribution and activity of protein phosphatase PP1gamma2 and its regulating proteins in spermatozoa lacking AKAP4Zaohua Huang
Department of Biological Sciences, Kent State University, Kent, Ohio 44242, USA
Biol Reprod 72:384-92. 2005..cAMP mediates its intracellular effects in spermatozoa through cAMP-dependent kinase (PKA, formally known as PRKACA)...
- Conservation of protein kinase a catalytic subunit sequences in the schistosome pathogens of humansBrett E Swierczewski
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814 4799, USA
Exp Parasitol 125:156-60. 2010..japonicum genomes contain sequences encoding for several putative PKA substrates with homology to those found in Homo sapiens, Caenorhabditis elegans, and Saccharomyces cerevisiae...
- Addicting drugs utilize a synergistic molecular mechanism in common requiring adenosine and Gi-beta gamma dimersLina Yao
Ernest Gallo Clinic and Research Center, 5858 Horton Street, Suite 200, Emeryville, CA 94608, USA
Proc Natl Acad Sci U S A 100:14379-84. 2003..neurons that a brief exposure to saturating concentrations of DOR and CB1 agonists increases cAMP, promotes PKA C alpha translocation and increases cAMP-dependent gene expression...
- Visual screening and analysis for kinase-regulated membrane trafficking pathways that are involved in extensive beta-amyloid secretionAtsuhiro Adachi
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902, Japan
Genes Cells 14:355-69. 2009..which examined the effect of kinase inhibitors and a kinase siRNA library, we identified five kinases (CDC42BPB, PRKACA, PRKACG, GSK3 beta and CSNK2A1) that regulate CI-M6PR trafficking...
- Proteomics analysis of plasma for potential biomarkers in the diagnosis of Alzheimer's diseasePao Chi Liao
Department of Environmental and Occupational Health, National Cheng Kung University, Tainan, Taiwan
Proteomics Clin Appl 1:506-12. 2007....
- Analysis of isoaspartate in peptides by electrospray tandem mass spectrometryW D Lehmann
Central Spectroscopy Unit, German Cancer Research Center DKFZ, Heidelberg, Germany
Protein Sci 9:2260-8. 2000..for which isoAsp formation has been described, namely myrG-D/isoD-AAAAK (deamidated peptide 1-7 of protein kinase A catalytic subunit) and VQ-D/isoD-GLR (deamidated peptide 41-46 of human procollagen alpha 1)...
- Signaling pathways for modulation of mouse sperm motility by adenosine and catecholamine agonistsSonya M Schuh
Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195, USA
Biol Reprod 74:492-500. 2006..are also absent in sperm from mice lacking the sperm-specific Calpha2 catalytic subunit of protein kinase A (PRKACA). Agonist responses also are strongly suppressed in wild-type sperm by the protein kinase inhibitor H-89...
- CDC25B acts as a potential target of PRKACA in fertilized mouse eggsCheng Cui
Department of Physiology, China Medical University, Shenyang 110001, China
Biol Reprod 79:991-8. 2008Protein kinase A (PRKACA) has been documented as a pivotal regulator in meiosis and mitosis arrest...
- Genes encoding catalytic subunits of protein kinase A and risk of spina bifidaHuiping Zhu
Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, Houston, Texas
Birth Defects Res A Clin Mol Teratol 73:591-6. 2005b>PRKACA and PRKACB are genes encoding the cAMP-dependent protein kinase A (PKA) catalytic subunits alpha and beta, respectively...
- Activity, expression and function of a second Drosophila protein kinase A catalytic subunit geneA Melendez
Department of Biological Sciences, Columbia University, New York, New York 10027, USA
Genetics 141:1507-20. 1995..These observations indicate that DC2 is not an essential gene and is unlikely to be functionally redundant with DC0, which has multiple unique functions during development...
- Phenotypic and transcriptomic analyses of Plasmodium falciparum protein kinase A catalytic subunit inhibitionNathalie Wurtz
UMR MD3, Relation Hôte Parasites Pharmacologie et Thérapeutique Université de la Méditerranée Aix Marseille II, des Armées Antenne Marseille, Institut de Recherche Biomédicale IMTSSA, Allée du Médecin Colonel Eugène Jamot, Parc du Pharo, Marseille, Cedex 07, France
Parasitol Res 105:1691-9. 2009....
- Epidermal growth factor receptor levels are reduced in mice with targeted disruption of the protein kinase A catalytic subunitMorten P Oksvold
Institute of Pathology, Rikshospitalet University Hospital, University of Oslo, Norway
BMC Cell Biol 9:16. 2008..Cyclic-AMP-dependent protein kinase consists of a regulatory dimer bound to two catalytic subunits...
- Cloning of a mouse protein kinase A catalytic subunit pseudogene and chromosomal mapping of C subunit isoformsD E Cummings
Department of Pharmacology, University of Washington, Seattle 98195
Mamm Genome 5:701-6. 1994Two isoforms of the protein kinase A catalytic subunit, C alpha and C beta, have previously been described in the mouse. We now report the cloning and characterization of a novel C-related sequence, Cx, from a murine genomic library...
- Structural analysis of ARC-type inhibitor (ARC-1034) binding to protein kinase A catalytic subunit and rational design of bisubstrate analogue inhibitors of basophilic protein kinasesDarja Lavogina
Institute of Chemistry, 2 Jakobi Street, 51014 Tartu, Estonia
J Med Chem 52:308-21. 2009The crystal structure of a complex of the catalytic subunit (type alpha) of cAMP-dependent protein kinase (PKA C alpha) with ARC-type inhibitor (ARC-1034), the presumed lead scaffold of previously reported adenosine-oligo-arginine ..
- Modulation of Drosophila slowpoke calcium-dependent potassium channel activity by bound protein kinase a catalytic subunitYi Zhou
Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Neurosci 22:3855-63. 2002..These results are consistent with the hypothesis that both binding of active PKAc to dSlo and phosphorylation of dSlo or some other protein are necessary for channel modulation...
- Identification of protein kinase A catalytic subunit beta as a novel binding partner of p73 and regulation of p73 functionTakayuki Hanamoto
Division of Biochemistry, Chiba Cancer Center Research Institute, Chiba 260 8717, Japan
J Biol Chem 280:16665-75. 2005..Using a yeast two-hybrid screening based on the Sos recruitment system, we identified protein kinase A catalytic subunit beta (PKA-Cbeta) as a novel binding partner of p73...
- Transcriptional regulation of cyclin D2 by the PKA pathway and inducible cAMP early repressor in granulosa cellsLuis C Muniz
Department of Biochemistry and Molecular Biology and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, New Jersey 07103, USA
Biol Reprod 75:279-88. 2006..The promoter activity of cyclin D2 was shown to be induced by FSH and the catalytic alpha subunit of PKA (PRKACA), and this activity was repressible by inducible cAMP early repressor (ICER), a cAMP response element (CRE) ..
- Expression and biochemical characterization of the Plasmodium falciparum protein kinase A catalytic subunitNathalie Wurtz
UMR MD3 UR3P, Relation hôte parasites Pharmacologie et Thérapeutique, Institut de Medecine Tropicale du Service de Sante des Armees, Marseille, France
Parasitol Res 104:1299-305. 2009..This work provides new insights on the study of PfPKAc that will contribute to future investigations of the parasite cAMP signaling pathway and to high-throughput screening of specific malarial PKA inhibitors...
- Stimulus-induced translocation of the protein kinase A catalytic subunit to the apical membrane in blowfly salivary glandsMartin Voss
Institut für Biochemie und Biologie, Universitat Potsdam, Karl Liebknecht Strasse 24 25, 14476 Potsdam, Germany
Cell Tissue Res 335:657-62. 2009..The recruitment of PKA-C to the apical membrane corroborates our hypothesis that vacuolar H(+)-ATPase, which is enriched in this membrane domain, is a target protein for PKA...
- Intracellular distribution of mammalian protein kinase A catalytic subunit altered by conserved Asn2 deamidationR Pepperkok
European Molecular Biology Laboratory, D 69012 Heidelberg, Germany
J Cell Biol 148:715-26. 2000..The model character for other signaling proteins starting with myrGly-Asn is discussed...
- Androgen-independent growth and tumorigenesis of prostate cancer cells are enhanced by the presence of PKA-differentiated neuroendocrine cellsPaul D Deeble
Department of Microbiology and Cancer Center, University of Virginia Health System, Charlottesville, VA 22908, USA
Cancer Res 67:3663-72. 2007..were engineered to inducibly express a constitutively activated form of the cyclic AMP-dependent protein kinase A catalytic subunit (caPKA), which was previously found upon transient transfection to be sufficient for acquisition of ..
- Genome-wide gain-of-function screen identifies novel regulators of pluripotencyRamzey Abujarour
Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA
Stem Cells 28:1487-97. 2010..1/Spi1, Prkaca, and Jun induced differentiation of ES cells...
- Uncoupling of bait-protein expression from the prey protein environment adds versatility for cell and tissue interaction proteomics and reveals a complex of CARP-1 and the PKA Cbeta1 subunitAndrea Erlbruch
Structural Biochemistry, German Cancer Research Center, Heidelberg, Germany
Proteomics 10:2890-900. 2010..the prey protein environment numerous established, mostly tissue-specific binding partners of the protein kinase A catalytic subunit Cbeta1 were identified, including interactions in binary, ternary and quaternary complexes...
- Protein phosphatase inhibitor-1 augments a protein kinase A-dependent increase in the Ca2+ loading of the sarcoplasmic reticulum without changing its Ca2+ releaseHirotaka Kawashima
Division of Cardiology, Internal Medicine III, Hamamatsu University School of Medicine, Hamamatsu 431 3192, Japan
Circ J 73:1133-40. 2009..An increase in cytosolic protein phosphatases (PPs) de-phosphorylates phospholamban, decreasing the Ca(2+) uptake of the sarcoplasmic reticulum (SR). The effects of PP inhibitors on cellular Ca(2+) handling were investigated...
- [Neuroprotective mechanism of tetrahydroxystilbene glucoside on rats after cerebral ischemia-reperfusion]Jie Yang
Department of Neurology, Xiangya Hospital, Central South University, Changsha 410078, China
Zhong Nan Da Xue Xue Bao Yi Xue Ban 35:321-8. 2010..To investigate the neuroprotective mechanism of tetrahydroxystilbene glucoside (TSG), a Chinese medicine, on rats after cerebral ischemia-reperfusion...
- The role of SF-1/Ad4BP in the control of the bovine gene for the steroidogenic acute regulatory (StAR) proteinW Rust
Institute for Hormone and Fertility Research, University of Hamburg, Germany
J Mol Endocrinol 21:189-200. 1998..Furthermore, additional cotransfection of a construct expressing a constitutive protein kinase A catalytic subunit to mimic the effects of cAMP stimulation, led to a small SF-1/Ad4BP-dependent increase in reporter ..
- Identifying all Meckel-like ciliopathy genes by established total exome capture aFriedhelm Hildebrandt; Fiscal Year: 2010..Using this approach we already identified mutations in SDCCAG8, PRKACA and MAP2 as novel causes of Meckel-like ciliopathies...
- INSULIN ACTION IN MUSCLE AND FAT CELLSJAMES CARLTON GARRISON; Fiscal Year: 2010..Lentivirus will be used to overexpress rictor or to express shRNA to knockdown rictor in 3T3-L1 adipocytes. To investigate mTORC2 function in vivo, we propose to knockout rictor in adipocytes and skeletal muscle of mice. ..
- Differentiation in T.cruzi:an emerging AIDS pathogenHuan Huang; Fiscal Year: 2009..To further delineate the components of this pathway, we will identify the TcPKA downstream interacting molecules, using both yeast two-hybrid assays and proteomic approaches. ..
- G-protein Regulation of the Phosphatidyl Inositol (3,4,5) Trisphosphate SignalJames Garrison; Fiscal Year: 2009..abstract_text> ..
- Cell Signaling by G Protein betagamma SubunitsJames Garrison; Fiscal Year: 2008..Parameters examined will include: receptor - alpha:betagamma interaction, adenylyl cyclase (cAMP), PLC-beta (Ca2+), Ptdlns 3-kinase (PIP3), P-Rex1 (Rac activation) and cell migration. ..
- Mutations in PKA gene transcripts of lupus T cellsDama Laxminarayana; Fiscal Year: 2007..abstract_text> ..
- VACM-1, cul 5 gene regulates cell growth & angiogenesis.Maria Burnatowska Hledin; Fiscal Year: 2004..Consequently, these results may lead to development of strategies for diagnosis and intervention in the pathologies of excessive vascularization and consequently cancer. ..
- MOLECULAR NEUROBIOLOGY OF DEPRESSIONRichard Shelton; Fiscal Year: 2003..Develop further expertise in basic molecular biological methods; 2. Develop new projects oriented to future funding opportunities; and 3. Continue to mentor the next generation of investigators. ..
- SEX STEROID REGULATION OF IGF-I EXPRESSION IN BONEThomas McCarthy; Fiscal Year: 2003..Bone mass studies will be conducted using peripheral quantitative computer tomography, and will be correlated with basal and activated skeletal IGF-I expression. ..
- Signal Transduction in DepressionRichard Shelton; Fiscal Year: 2008..These studies may shed light on important cellular mediators of depression and provide new targets for amelioration. [unreadable] [unreadable]..
- Mechanisms directing oncoprotein and cytokine mRNA decayGERALD WILSON; Fiscal Year: 2008..Together, these studies will further our understanding of the relationships between ARE structure, trans-factor recognition, and the cellular functions of resulting RNP complexes. ..