PRKACA

Summary

Gene Symbol: PRKACA
Description: protein kinase, cAMP-dependent, catalytic, alpha
Alias: PKACA, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha, protein kinase A catalytic subunit
Species: human

Top Publications

  1. ncbi 3-ketosteroid reductase activity and expression by fetal rat osteoblasts
    Thomas L McCarthy
    Department of Surgery, Section of Plastic Surgery, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
    J Biol Chem 282:34003-12. 2007
  2. doi Melanocyte-lineage expression of Cre recombinase using Mitf regulatory elements
    Azita Alizadeh
    Departments of Genetics and Pediatrics, Stanford University School of Medicine, Stanford, CA, USA
    Pigment Cell Melanoma Res 21:63-9. 2008
  3. pmc Odorant receptors at the growth cone are coupled to localized cAMP and Ca2+ increases
    Micol Maritan
    Venetian Institute of Molecular Medicine, Via Orus 2, 35129 Padova, Italy
    Proc Natl Acad Sci U S A 106:3537-42. 2009
  4. pmc Protein kinase A catalytic subunit interacts and phosphorylates members of trans-sialidase super-family in Trypanosoma cruzi
    Yi Bao
    Department of Pathology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Microbes Infect 12:716-26. 2010
  5. pmc The N terminus of adenovirus type 12 E1A inhibits major histocompatibility complex class I expression by preventing phosphorylation of NF-kappaB p65 Ser276 through direct binding
    Junfang Jiao
    Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Virol 84:7668-74. 2010
  6. pmc Transcriptional activation of the NF-kappaB p65 subunit by mitogen- and stress-activated protein kinase-1 (MSK1)
    Linda Vermeulen
    Department of Molecular Biology, University of Gent VIB, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    EMBO J 22:1313-24. 2003
  7. ncbi Regulation of the ER81 transcription factor and its coactivators by mitogen- and stress-activated protein kinase 1 (MSK1)
    Ralf Janknecht
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
    Oncogene 22:746-55. 2003
  8. ncbi cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes
    S S Taylor
    Department of Chemistry, University of California, San Diego, La Jolla 92093
    Annu Rev Biochem 59:971-1005. 1990
  9. pmc Protein kinase A binds and activates heat shock factor 1
    Ayesha Murshid
    Molecular and Cellular Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 5:e13830. 2010
  10. pmc Protein kinase A phosphorylation activates Vpr-induced cell cycle arrest during human immunodeficiency virus type 1 infection
    R Anthony Barnitz
    Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 10 Center Dr, Bethesda, MD 20892 1892, USA
    J Virol 84:6410-24. 2010

Research Grants

  1. Mechanisms directing oncoprotein and cytokine mRNA decay
    GERALD WILSON; Fiscal Year: 2007
  2. INSULIN ACTION IN MUSCLE AND FAT CELLS
    James Garrison; Fiscal Year: 2007
  3. Differentiation in T.cruzi:an emerging AIDS pathogen
    Huan Huang; Fiscal Year: 2007
  4. Signal Transduction in Depression
    Richard Shelton; Fiscal Year: 2007
  5. CELL SIGNALING REGULATION BY G PROTEIN SUBUNITS
    James Garrison; Fiscal Year: 2005
  6. Mutations in PKA gene transcripts of lupus T cells
    Dama Laxminarayana; Fiscal Year: 2007
  7. Cell Signaling by G Protein betagamma Subunits
    James Garrison; Fiscal Year: 2007
  8. INSULIN ACTION IN MUSCLE AND FAT CELLS
    JAMES CARLTON GARRISON; Fiscal Year: 2010
  9. Mechanisms directing oncoprotein and cytokine mRNA decay
    Gerald M Wilson; Fiscal Year: 2010
  10. Protective role of ANP in the models of acute lung injury
    Anna Birukova; Fiscal Year: 2010

Detail Information

Publications177 found, 100 shown here

  1. ncbi 3-ketosteroid reductase activity and expression by fetal rat osteoblasts
    Thomas L McCarthy
    Department of Surgery, Section of Plastic Surgery, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
    J Biol Chem 282:34003-12. 2007
    ..AKR1C9 promoter activity was also enhanced by overexpression of protein kinase A catalytic subunit or transcription factor C/EBPdelta, and the effect of PGE2 was reduced by dominant negative C/..
  2. doi Melanocyte-lineage expression of Cre recombinase using Mitf regulatory elements
    Azita Alizadeh
    Departments of Genetics and Pediatrics, Stanford University School of Medicine, Stanford, CA, USA
    Pigment Cell Melanoma Res 21:63-9. 2008
    ..utility of the MitfCre transgene, we used a loxP-targeted allele of the protein kinase A alpha catalytic subunit (Prkaca), modified such that Cre-mediated recombination activates PKA signaling...
  3. pmc Odorant receptors at the growth cone are coupled to localized cAMP and Ca2+ increases
    Micol Maritan
    Venetian Institute of Molecular Medicine, Via Orus 2, 35129 Padova, Italy
    Proc Natl Acad Sci U S A 106:3537-42. 2009
    ..that selective odor activation of the OR on the growth cone is followed by nuclear translocation of protein kinase A catalytic subunit. These results define the functional properties of the OR on the growth cone and suggest a potential ..
  4. pmc Protein kinase A catalytic subunit interacts and phosphorylates members of trans-sialidase super-family in Trypanosoma cruzi
    Yi Bao
    Department of Pathology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Microbes Infect 12:716-26. 2010
    ..The identified trans-sialidases have PKA consensus phosphorylation sites located near the endoplasmic reticulum retention motif in the N-terminal. These data support that PKA phosphorylates trans-sialidase super family members in vivo...
  5. pmc The N terminus of adenovirus type 12 E1A inhibits major histocompatibility complex class I expression by preventing phosphorylation of NF-kappaB p65 Ser276 through direct binding
    Junfang Jiao
    Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Virol 84:7668-74. 2010
    ..by the Ad12 E1A protein (E1A-12), which prevents NF-kappaB from becoming phosphorylated by the protein kinase A catalytic subunit (PKAc). In this study, we examined the interactions between E1A-12 and NF-kappaB...
  6. pmc Transcriptional activation of the NF-kappaB p65 subunit by mitogen- and stress-activated protein kinase-1 (MSK1)
    Linda Vermeulen
    Department of Molecular Biology, University of Gent VIB, K L Ledeganckstraat 35, B 9000 Gent, Belgium
    EMBO J 22:1313-24. 2003
    ..This effect represents, together with phosphorylation of nucleosome components such as histone H3, an essential step leading to selective transcriptional activation of NF-kappaB-dependent gene expression...
  7. ncbi Regulation of the ER81 transcription factor and its coactivators by mitogen- and stress-activated protein kinase 1 (MSK1)
    Ralf Janknecht
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
    Oncogene 22:746-55. 2003
    ..Thus, MSK1 may regulate ER81-dependent transcription via direct phosphorylation of ER81 as well as via stimulation of CBP/p300, which might be important for ER81's normal function and during mammary tumor formation...
  8. ncbi cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes
    S S Taylor
    Department of Chemistry, University of California, San Diego, La Jolla 92093
    Annu Rev Biochem 59:971-1005. 1990
    ....
  9. pmc Protein kinase A binds and activates heat shock factor 1
    Ayesha Murshid
    Molecular and Cellular Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 5:e13830. 2010
    ..As the mechanisms involved in the intracellular regulation of HSF1 in good health and its dysregulation in disease are still incomplete we are investigating the role of posttranslational modifications in such regulation...
  10. pmc Protein kinase A phosphorylation activates Vpr-induced cell cycle arrest during human immunodeficiency virus type 1 infection
    R Anthony Barnitz
    Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 10 Center Dr, Bethesda, MD 20892 1892, USA
    J Virol 84:6410-24. 2010
    ..Inhibition of PKA activity during HIV-1 infection abrogates Vpr cell cycle arrest. These findings provide new insight into the signaling event that activates Vpr cell cycle arrest, ultimately leading to the death of infected T cells...
  11. ncbi The transcriptional activity of NF-kappaB is regulated by the IkappaB-associated PKAc subunit through a cyclic AMP-independent mechanism
    H Zhong
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Cell 89:413-24. 1997
    ..Therefore, this pathway represents a novel mechanism for the cAMP-independent activation of PKA and the regulation of NF-kappaB activity...
  12. ncbi A novel isoform of human cyclic 3',5'-adenosine monophosphate-dependent protein kinase, c alpha-s, localizes to sperm midpiece
    N Reinton
    Institute of Medical Biochemistry, University of Oslo, N 0317 Oslo, Norway
    Biol Reprod 63:607-11. 2000
    ..This novel C alpha-s splice variant appeared to have an independent anchor in the human sperm midpiece as it could not be completely solubilized even in the presence of both detergent and cAMP...
  13. pmc Cyclic AMP-dependent protein kinase regulates the alternative splicing of tau exon 10: a mechanism involved in tau pathology of Alzheimer disease
    Jianhua Shi
    Jiangsu Key Laboratory of Neuroregeneration, Medical School, Nantong University, Nantong, Jiangsu 226001, China
    J Biol Chem 286:14639-48. 2011
    ..These findings suggest that a down-regulation of PKA dysregulates the alternative splicing of tau exon 10 and contributes to neurofibrillary degeneration in AD by causing an imbalance in 3R-tau and 4R-tau expression...
  14. ncbi Proteasome function is regulated by cyclic AMP-dependent protein kinase through phosphorylation of Rpt6
    Fengxue Zhang
    Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, University of Alabama, Birmingham, Alabama 35294, USA
    J Biol Chem 282:22460-71. 2007
    ..Thus, hormones using the PKA system can also regulate proteasomes often in concert with glucose metabolism. This finding might lead to novel strategies for the treatment of proteasome-related diseases...
  15. pmc Inhibiting TRAF2-mediated activation of NF-kappaB facilitates induction of AP-1
    Sunil K Manna
    Laboratory of Immunology, Centre for DNA Fingerprinting and Diagnostics, Nampally, Hyderabad 500 001, India
    J Biol Chem 285:11617-27. 2010
    ..These results suggest novel approaches to design of P(3)-25 as an anti-cancer/inflammatory drug for therapy through regulation of the TRAF2 pathway...
  16. pmc Prostaglandin E2 inhibits specific lung fibroblast functions via selective actions of PKA and Epac-1
    Steven K Huang
    Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109 5642, USA
    Am J Respir Cell Mol Biol 39:482-9. 2008
    ..The selective use of PKA and Epac-1 pathways to inhibit distinct aspects of fibroblast activation illustrate the pleiotropic ability of PGE(2) to inhibit diverse fibroblast functions...
  17. ncbi Serine phosphorylation negatively regulates RhoA in vivo
    Shawn M Ellerbroek
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 278:19023-31. 2003
    ....
  18. ncbi Expression, purification, and regulation of two isoforms of the inositol 1,4,5-trisphosphate 3-kinase
    P J Woodring
    Department of Pharmacology and Cancer Research Center, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    J Biol Chem 272:30447-54. 1997
    ..Differential expression and regulation of the two inositol 1,4,5-trisphosphate 3-kinase isoforms provides multiple mechanisms for regulating the cytosolic level of inositol 1,4,5-trisphosphate in cells...
  19. pmc Parkinsonian neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and alpha-synuclein mutations promote Tau protein phosphorylation at Ser262 and destabilize microtubule cytoskeleton in vitro
    Hamid Y Qureshi
    Department of Neurology and Neurosurgery, McGill University, Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Quebec H3T 1E2, Canada
    J Biol Chem 286:5055-68. 2011
    ....
  20. pmc Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259
    Amardeep S Dhillon
    The Beatson Institute for Cancer Research, Bearsden, Glasgow G61 1BD, United Kingdom
    Mol Cell Biol 22:3237-46. 2002
    ..Using RafS259 mutants we also demonstrate that Raf-1 is the sole target for PKA inhibition of ERK and ERK-induced gene expression, and that Raf-1 inhibition is mediated mainly through serine 259 phosphorylation...
  21. doi CD9 regulates transcription factor GCM1 and ERVWE1 expression through the cAMP/protein kinase A signaling pathway
    Yoshikage Muroi
    Laboratory of Animal Breeding, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, 113 8657 Tokyo, Japan
    Reproduction 138:945-51. 2009
    ..Moreover, CD9-induced increase in ERVWE1 and GCM1 expressions were inhibited by Rp-cAMPS. These results suggest that CD9 increases GCM1 expression via the cAMP/PKA signaling pathway, resulting in the increase in ERVWE1 expression...
  22. pmc Protein kinase A is central for forward transport of two-pore domain potassium channels K2P3.1 and K2P9.1
    Alexandra Mant
    Division of Human Genetics, Centre for Human Development, Stem Cells and Regeneration, University of Southampton, Duthie Building, Mail Point 808, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, United Kingdom
    J Biol Chem 286:14110-9. 2011
    ..All of our findings support the conclusion that cAMP-dependent protein kinase is responsible for the phosphorylation of the terminal serine in both K2P3.1 and K2P9.1...
  23. ncbi Phosphorylation of the inositol 1,4,5-trisphosphate receptor by cyclic GMP-dependent protein kinase
    P Komalavilas
    Department of Pathology, University of Alabama, Birmingham 35294 0019
    J Biol Chem 269:8701-7. 1994
    ..Phosphorylation of IP3 receptor by cGMP kinase may regulate the function of IP3 receptor in vascular smooth muscle cells and contribute to the effect of cGMP to regulate intracellular calcium levels...
  24. pmc cAMP-dependent protein kinase phosphorylation of the acid-sensing ion channel-1 regulates its binding to the protein interacting with C-kinase-1
    A Soren Leonard
    Department of Internal Medicine, Roy J and Lucille A Carver College of Medicine, Howard Hughes Medical Institute, University of Iowa, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 100:2029-34. 2003
    ..Thus, the ASIC1 C terminus contains two sites that influence its binding to PICK1. Regulation of this interaction by phosphorylation provides a mechanism to control the cellular localization of ASIC1...
  25. pmc The testis-specific Cα2 subunit of PKA is kinetically indistinguishable from the common Cα1 subunit of PKA
    Maike M Vetter
    Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, PB 1046 Blindern, Oslo, Norway
    BMC Biochem 12:40. 2011
    ..the α-form of the catalytic (C) subunit of protein kinase A (PKA), designated Cα1 and Cα2, are encoded by the PRKACA gene. Whereas Cα1 is ubiquitous, Cα2 expression is restricted to the sperm cell...
  26. doi Viral genetic determinants of nonprogressive HIV type 1 subtype C infection in antiretroviral drug-naive children
    D B Tzitzivacos
    HIV Pathogenesis Research Laboratory, Department of Molecular Medicine and Haematology, University of the Witwatersrand Medical School, Parktown, Johannesburg 2193, South Africa
    AIDS Res Hum Retroviruses 25:1141-8. 2009
    ..For example, LT46 Nef is unable to bind AP-1 and AP-2 and therefore is inactive on CD4 endocytosis. The biological relevance of these findings requires further investigation...
  27. ncbi Mammalian histone deacetylase 1 protein is posttranslationally modified by phosphorylation
    R Cai
    Functional Genomics, Novartis Pharmaceuticals Corporation, Summit, New Jersey 07901, USA
    Biochem Biophys Res Commun 283:445-53. 2001
    ..Interestingly, HDAC1-FLAG fusion protein immunoprecipitated from transfected cells was found to be in association with a kinase activity, providing an in vitro assay for further studies of this posttranslational modification...
  28. ncbi Interleukin-3 and bryostatin-1 mediate hyperphosphorylation of BCL2 alpha in association with suppression of apoptosis
    W S May
    Johns Hopkins Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231
    J Biol Chem 269:26865-70. 1994
    ..These findings strongly support a role for activated PKC in growth factor-induced Bc12 alpha phosphorylation as well as suppression of apoptosis...
  29. pmc 14-3-3 proteins are required for maintenance of Raf-1 phosphorylation and kinase activity
    J A Thorson
    Center for Immunology and Department of Pathology, Washington University School of Medicine, St Louis, Missouri, USA
    Mol Cell Biol 18:5229-38. 1998
    ..This supports a paradigm in which the effects of phosphorylation on serine as well as tyrosine residues are mediated by inducible protein-protein interactions...
  30. ncbi Protein kinase A regulates caspase-9 activation by Apaf-1 downstream of cytochrome c
    Morag C Martin
    Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Scotland, United Kingdom
    J Biol Chem 280:15449-55. 2005
    ..These data indicate that extracellular signals that elevate cyclic AMP and activate protein kinase A may suppress apoptosis by inhibiting apoptosome formation downstream of cytochrome c release from mitochondria...
  31. pmc Scar/WAVE-1, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffold
    R S Westphal
    Howard Hughes Medical Institute, Vollum Institute, Portland, OR, USA
    EMBO J 19:4589-600. 2000
    ..Thus, we propose a previously unrecognized function for WAVE-1 as an actin-associated scaffolding protein that recruits PKA and Abl...
  32. ncbi Dissecting the cooperative reassociation of the regulatory and catalytic subunits of cAMP-dependent protein kinase. Role of Trp-196 in the catalytic subunit
    R M Gibson
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093 0654, USA
    J Biol Chem 272:31998-2005. 1997
    ..One of these mutants, rR(R333K), having a defect in cAMP binding site B formed a stable complex with rC(W196R) in the absence of cAMP. However, unlike wild-type holoenzyme, this complex was active...
  33. ncbi Involvement of the catalytic subunit of protein kinase A and of HA95 in pre-mRNA splicing
    Anne Katrine Kvissel
    Department of Nutrition, University of Oslo, Oslo, Norway
    Exp Cell Res 313:2795-809. 2007
    ..Our findings demonstrate that the nuclear PKA C subunit co-locates with HA95 in SFCs and regulates pre-mRNA splicing, possibly through a cAMP-independent mechanism...
  34. ncbi Crosstalk between cAMP-dependent kinase and MAP kinase through a protein tyrosine phosphatase
    M Saxena
    Laboratory of Signal Transduction, Sidney Kimmel Cancer Center, San Diego, California 92121, USA
    Nat Cell Biol 1:305-11. 1999
    ..We conclude that HePTP not only controls the activity of MAP kinases, but also mediates crosstalk between the cAMP system and the MAP-kinase cascade...
  35. ncbi Phosphorylation and activation of a cAMP-specific phosphodiesterase by the cAMP-dependent protein kinase. Involvement of serine 54 in the enzyme activation
    C Sette
    Division of Reproductive Biology, Department of Gynecology and Obstetrics, Stanford University Medical Center, Stanford, California 94305 5317, USA
    J Biol Chem 271:16526-34. 1996
    ..These findings support the hypothesis that the PDE4D3 variant contains a regulatory domain target for phosphorylation at the amino terminus of the protein and that Ser54 in this domain plays a crucial role in activation...
  36. pmc Stimulation of GCMa transcriptional activity by cyclic AMP/protein kinase A signaling is attributed to CBP-mediated acetylation of GCMa
    Ching Wen Chang
    Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 115, Taiwan
    Mol Cell Biol 25:8401-14. 2005
    ..Our results reveal a novel regulation of GCMa activity by cAMP-dependent protein acetylation and provide a molecular mechanism by which cAMP signaling regulates trophoblastic fusion...
  37. ncbi Identification of novel phosphorylation sites in hormone-sensitive lipase that are phosphorylated in response to isoproterenol and govern activation properties in vitro
    M W Anthonsen
    Department of Cell and Molecular Biology, Section for Molecular Signaling, Lund University, S 221 00 Lund, Sweden
    J Biol Chem 273:215-21. 1998
    ....
  38. ncbi Properties and phosphorylation sites of baculovirus-expressed nuclear inhibitor of protein phosphatase-1 (NIPP-1)
    V Vulsteke
    Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 272:32972-8. 1997
    ....
  39. ncbi VACM-1, a cullin gene family member, regulates cellular signaling
    M Burnatowska-Hledin
    Departments of Biology and Chemistry, Peale Science Center, Hope College, Holland, Michigan 49422 9000, USA
    Am J Physiol Cell Physiol 279:C266-73. 2000
    ..Our data demonstrate that VACM-1, the first mammalian cullin protein to be characterized, is involved in the regulation of signaling...
  40. ncbi Two distinct caveolin-1 domains mediate the functional interaction of caveolin-1 with protein kinase A
    B Razani
    Department of Molecular Pharmacology and The Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Cell Physiol 281:C1241-50. 2001
    ..e., the mutant acts in a dominant-negative fashion. Thus, this dominant-negative caveolin-1 mutant will be extremely valuable for assessing the functional role of endogenous caveolin-1 in regulating a variety of other signaling cascades...
  41. ncbi Phosphorylation of inositol 1,4,5-trisphosphate receptors in parotid acinar cells. A mechanism for the synergistic effects of cAMP on Ca2+ signaling
    Jason I E Bruce
    Department of Pharmacology and Physiology, School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 277:1340-8. 2002
    ..In addition, this report supports the emerging consensus that phosphorylation at the level of the Ca(2+) release machinery is a broadly important mechanism by which cells can regulate Ca(2+)-mediated processes...
  42. ncbi HIV-1 gp120 induces anergy in naive T lymphocytes through CD4-independent protein kinase-A-mediated signaling
    Anna Maria Masci
    Dipartimento di Biologia e Patologia Cellulare e Molecolare, Universita di Napoli Federico II, 5 Via S Pansini, I 80131 Naples, Italy
    J Leukoc Biol 74:1117-24. 2003
    ..These findings reveal a novel mechanism by which HIVenv may modulate the processes of clonal expansion, homeostatic proliferation, and terminal differentiation of the naive T lymphocyte subset...
  43. pmc Cyclic AMP blocks cell growth through Raf-1-dependent and Raf-1-independent mechanisms
    Nicolas Dumaz
    Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, London SW3 6JB, United Kingdom
    Mol Cell Biol 22:3717-28. 2002
    ..Thus, although Raf-1 appears to be an important cAMP target, other pathways are also targeted by cAMP, providing alternative mechanisms that lead to suppression of cell growth...
  44. ncbi Regulation of the CDK-related protein kinase PCTAIRE-1 and its possible role in neurite outgrowth in Neuro-2A cells
    Ralph Graeser
    ICRF Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    J Cell Sci 115:3479-90. 2002
    ....
  45. ncbi Effect of phosphorylation on activities of Rap1A to interact with Raf-1 and to suppress Ras-dependent Raf-1 activation
    C D Hu
    Department of Physiology II, Kobe University School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    J Biol Chem 274:48-51. 1999
    ....
  46. ncbi Mitotic phosphorylation of the Oct-1 homeodomain and regulation of Oct-1 DNA binding activity
    N Segil
    Laboratory of Molecular Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021
    Science 254:1814-6. 1991
    ..The inhibition of Oct-1 DNA binding during mitosis suggests a mechanism by which the general inhibition of transcription during mitosis might occur...
  47. doi Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif
    Kotaro Shirakawa
    Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaracho, Sakyo ku, Kyoto 606 8507, Japan
    Nat Struct Mol Biol 15:1184-91. 2008
    ..These data imply that PKA-mediated phosphorylation of A3G can regulate the interaction between A3G and Vif...
  48. pmc Expression of a nonmyristylated variant of the catalytic subunit of protein kinase A during male germ-cell development
    J L Desseyn
    Department of Pharmacology, University of Washington, Seattle, WA 98195 7750, USA
    Proc Natl Acad Sci U S A 97:6433-8. 2000
    ..Because protein kinase A is thought to play a pivotal role in sperm motility and capacitation, the distinctive biochemical properties of the unmyristylated Calpha2 may be essential for fertility in the male...
  49. ncbi Adducin regulation. Definition of the calmodulin-binding domain and sites of phosphorylation by protein kinases A and C
    Y Matsuoka
    Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 271:25157-66. 1996
    ..These findings suggest a complex reciprocal relationship between regulation of adducin function by calmodulin binding and phosphorylation by PKA and PKC...
  50. pmc Protein kinase A gating of a pseudopodial-located RhoA/ROCK/p38/NHE1 signal module regulates invasion in breast cancer cell lines
    Rosa A Cardone
    Department of General and Environmental Physiology, University of Bari, 70126 Bari, Italy
    Mol Biol Cell 16:3117-27. 2005
    ....
  51. pmc A-kinase-interacting protein localizes protein kinase A in the nucleus
    Mira Sastri
    The Howard Hughes Medical Institute and Departments of Chemistry and Biochemistry and Pharmacology, University of California at San Diego, La Jolla, CA 92093 0654, USA
    Proc Natl Acad Sci U S A 102:349-54. 2005
    ..Thus, AKIP1 describes a PKA-interacting protein that can contribute to localization by a mechanism that is distinct from A-kinase anchoring proteins that interact with the regulatory subunits...
  52. ncbi Active cAMP-dependent protein kinase incorporated within highly purified HIV-1 particles is required for viral infectivity and interacts with viral capsid protein
    Christine Cartier
    Laboratoire Infections Retrovirales et Signalisation Cellulaire, Centre National de la Recherche Scientifique, UMR 5121 UM1, Institut de Biologie, CS 89508, 34960 Montpellier Cedex 2, France
    J Biol Chem 278:35211-9. 2003
    ..Altogether our results indicate that virus-associated C-PKA regulates HIV-1 infectivity, possibly by catalyzing phosphorylation of the viral CAp24gag protein...
  53. ncbi Identification of Raf-1 Ser621 kinase activity from NIH 3T3 cells as AMP-activated protein kinase
    A B Sprenkle
    Howard Hughes Medical Institute, Department of Medicine, University of Virginia, Charlottesville 22908, USA
    FEBS Lett 403:254-8. 1997
    ..AMPK also phosphorylated full-length, kinase-defective Raf-1 (K375M) to generate two [32P]phosphopeptides, one co-migrating with synthetic tryptic peptide containing phospho-Ser621 and the other with phospho-Ser259...
  54. pmc D-myo-inositol 1,4,5-trisphosphate 3-kinase A is activated by receptor activation through a calcium:calmodulin-dependent protein kinase II phosphorylation mechanism
    D Communi
    Institute of Interdisciplinary Research, Free University of Brussels, Belgium
    EMBO J 16:1943-52. 1997
    ..In this study, direct evidence is provided for a novel regulation mechanism for Ins(1,4,5)P3 3-kinase (isoform A) in vitro and in intact cells...
  55. pmc Control of rat GluR6 glutamate receptor open probability by protein kinase A and calcineurin
    S F Traynelis
    Department of Pharmacology, Rollins Research Center, Emory University, Atlanta, GA 30322 3090, USA
    J Physiol 503:513-31. 1997
    ..0) and calcineurin (Po,peak near 0.5)...
  56. doi The intermediate filament vimentin regulates chondrogenesis of adult human bone marrow-derived multipotent progenitor cells
    Brent E Bobick
    Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA
    J Cell Biochem 109:265-76. 2010
    ..In conclusion, we describe a novel role for the intermediate filament vimentin as a positive regulator of adult human bone marrow-derived MPC chondrogenesis...
  57. ncbi Apolipoprotein A-I activates cellular cAMP signaling through the ABCA1 transporter
    Bassam Haidar
    Cardiovascular Genetics Laboratory, Division of Cardiology, McGill University Health Centre Royal Victoria Hospital, Montreal, Quebec H3A 1A1, Canada
    J Biol Chem 279:9963-9. 2004
    ..In contrast, the protein kinase A catalytic subunit was able to phosphorylate ABCA1 similarly from mutant and normal cell lines in vitro...
  58. ncbi Protein kinase catalytic subunit (PKAcat) from bovine lens: purification, characterization and phosphorylation of lens crystallins
    Bhaswati Samanta
    Department of Chemistry, Bose Institute, 93 1, A P C Road, Kolkata 700009, India
    Mol Cell Biochem 304:155-65. 2007
    The purification and functional characterization of protein kinase A catalytic subunit (PKAcat) from bovine lens cytosol has been described...
  59. ncbi Functional characterization of two distinct Mg(2+) extrusion mechanisms in cardiac sarcolemmal vesicles
    Christie Cefaratti
    Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 4970, USA
    Mol Cell Biochem 303:63-72. 2007
    ..6) Na(+)-dependent Mg(2+) transport could be restored by stimulating vesicles loaded with protein kinase A catalytic subunit and ATP with membrane-permeant cyclic-AMP analog; (7) extra-vesicular Mg(2+) could be accumulated in ..
  60. ncbi Proteasome-driven turnover of tryptophan hydroxylase is triggered by phosphorylation in RBL2H3 cells, a serotonin producing mast cell line
    Yoshiko Iida
    Department of Bioscience, and Biotechnology Research Center, Teikyo University of Science and Technology, Yamanashi, Japan
    Eur J Biochem 269:4780-8. 2002
    ..These results indicated that the phosphorylation of TPH was a prerequisite for proteasome-driven TPH degradation...
  61. pmc Hemocytes are essential for wing maturation in Drosophila melanogaster
    J A Kiger
    Molecular and Cellular Biology, University of California, 1 Shields Avenue, Davis, CA 95616, USA
    Proc Natl Acad Sci U S A 98:10190-5. 2001
    ..Second, ectopic expression of protein kinase A catalytic subunit (PKAc) using certain GAL4 enhancer detector strains mimics the batone wing phenotype and also ..
  62. ncbi Molecular cloning and expression of the catalytic subunit of protein kinase A from Trypanosoma cruzi
    Huan Huang
    Department of Pathology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Int J Parasitol 32:1107-15. 2002
    ..protein kinases was utilised to amplify regions corresponding to the parasite homologue of the protein kinase A catalytic subunit. This putative protein kinase A fragment was used to isolate the entire gene from T...
  63. ncbi G protein mutations in pituitary tumors: a study on Turkish patients
    Beki Kan
    Department of Biophysics, Marmara University School of Medicine, Istanbul, Turkey
    Pituitary 6:75-80. 2003
    ..in pituitary tumors from Turkish patients and to investigate the possibility of mutations of protein kinase A catalytic subunit (PKAC) that activates the downstream effectors of adenylyl cyclase...
  64. doi The use of phosphate-affinity SDS-PAGE to measure the cardiac troponin I phosphorylation site distribution in human heart muscle
    Andrew E Messer
    National Heart and Lung Institute, Imperial College London, London, UK
    Proteomics Clin Appl 3:1371-82. 2009
    ..To test the method we phosphorylated pure cTnI with protein kinase A catalytic subunit and observed up to six bands corresponding to 0, 1P, 2P, 3P, 4P and 5P phospho-species...
  65. ncbi Paired-like homeodomain proteins Phox2a/Arix and Phox2b/NBPhox have similar genetic organization and independently regulate dopamine beta-hydroxylase gene transcription
    M Adachi
    Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, Portland 97201, USA
    DNA Cell Biol 19:539-54. 2000
    ..from the rat DBH promoter, and transcription is synergistically increased in the presence of the protein kinase A catalytic subunit (PKA) plus either Arix or NBPhox...
  66. pmc Properties of the cAMP-activated C1- current in choroid plexus epithelial cells isolated from the rat
    J D Kibble
    Cell Physiology Group, School of Biological Sciences, University of Manchester, UK
    J Physiol 496:69-80. 1996
    ..2. In patch clamp experiments, inclusion of 0.25 mM cAMP and 375 protein kinase A catalytic subunit (PKA) in the electrode solution caused activation of an inwardly rectifying current (21/23 cells)...
  67. doi Gene and microRNA expression in p53-deficient day 8.5 mouse embryos
    Hiromi Hosako
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, 434 VMR Building, College Station, TX 77843, USA
    Birth Defects Res A Clin Mol Teratol 85:546-55. 2009
    ..Here we attempt to identify genes and microRNAs (miRNAs) modified by deletion of p53 in day 8.5 mouse embryos...
  68. ncbi Mucor rouxii Rho1 protein; characterization and possible role in polarized growth
    Silvia Argimon
    Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellon 2, Piso 4, 1428, Buenos Aires, Argentina
    Antonie Van Leeuwenhoek 91:237-51. 2007
    ..The peptide IRRNSQKFV, containing Ser135 proved to be a good substrate for M. rouxii protein kinase A catalytic subunit. The over-expressed Rho1 fully complements a Saccharomyces cerevisiae null mutant...
  69. ncbi Protein kinase A catalytic subunit alters cardiac mitochondrial redox state and membrane potential via the formation of reactive oxygen species
    Shiro Nagasaka
    Division of Cardiology, Internal Medicine III, Hamamatsu University School of Medicine, Hamamatsu, Japan
    Circ J 71:429-36. 2007
    ..The identification of protein kinase A (PKA) anchoring proteins on mitochondria implies a direct effect of PKA on mitochondrial function. However, little is known about the relationship between PKA and mitochondrial metabolism...
  70. pmc A conserved deamidation site at Asn 2 in the catalytic subunit of mammalian cAMP-dependent protein kinase detected by capillary LC-MS and tandem mass spectrometry
    P T Jedrzejewski
    Department of Central Spectroscopy, German Cancer Research Center, Heidelberg
    Protein Sci 7:457-69. 1998
    ..This may be especially significant in view of the recent knowledge that negative charges close to myristic acid in some proteins contribute to regulating their cellular localization...
  71. ncbi Ca2+/Calmodulin-dependent protein kinase II phosphorylation of ryanodine receptor does affect calcium sparks in mouse ventricular myocytes
    Tao Guo
    Department of Physiology and Cardiovascular Institute, Loyola University Chicago, Stritch School of Medicine, Maywood, IL 60153, USA
    Circ Res 99:398-406. 2006
    ..This effect was abolished by CaMKII inhibitor peptides. In contrast, protein kinase A catalytic subunit also enhanced Ca2+ spark frequency in WT, but had no effect in PLB-KO...
  72. pmc Conservation of protein kinase a catalytic subunit sequences in the schistosome pathogens of humans
    Brett E Swierczewski
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814 4799, USA
    Exp Parasitol 125:156-60. 2010
    ..japonicum genomes contain sequences encoding for several putative PKA substrates with homology to those found in Homo sapiens, Caenorhabditis elegans, and Saccharomyces cerevisiae...
  73. pmc Sperm-specific protein kinase A catalytic subunit Calpha2 orchestrates cAMP signaling for male fertility
    Michael A Nolan
    Department of Pharmacology, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 101:13483-8. 2004
    ..These results define several crucial roles of PKA in sperm cell biology, bringing together both known and unique PKA-mediated events that are necessary for male fertility...
  74. ncbi Changes in intracellular distribution and activity of protein phosphatase PP1gamma2 and its regulating proteins in spermatozoa lacking AKAP4
    Zaohua Huang
    Department of Biological Sciences, Kent State University, Kent, Ohio 44242, USA
    Biol Reprod 72:384-92. 2005
    ..cAMP mediates its intracellular effects in spermatozoa through cAMP-dependent kinase (PKA, formally known as PRKACA)...
  75. ncbi Activation of cAMP-dependent signaling pathway induces mouse organic anion transporting polypeptide 2 expression
    Chuan Chen
    Department of Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    Mol Pharmacol 71:1159-64. 2007
    ..promoter containing this CRE site was induced in cells cotransfected with a plasmid encoding the protein kinase A catalytic subunit. Cotransfection of cells with a plasmid encoding the dominant-negative CRE binding protein (CREB) ..
  76. doi Proteomics analysis of plasma for potential biomarkers in the diagnosis of Alzheimer's disease
    Pao Chi Liao
    Department of Environmental and Occupational Health, National Cheng Kung University, Tainan, Taiwan
    Proteomics Clin Appl 1:506-12. 2007
    ....
  77. doi Visual screening and analysis for kinase-regulated membrane trafficking pathways that are involved in extensive beta-amyloid secretion
    Atsuhiro Adachi
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902, Japan
    Genes Cells 14:355-69. 2009
    ..which examined the effect of kinase inhibitors and a kinase siRNA library, we identified five kinases (CDC42BPB, PRKACA, PRKACG, GSK3 beta and CSNK2A1) that regulate CI-M6PR trafficking...
  78. pmc Analysis of isoaspartate in peptides by electrospray tandem mass spectrometry
    W D Lehmann
    Central Spectroscopy Unit, German Cancer Research Center DKFZ, Heidelberg, Germany
    Protein Sci 9:2260-8. 2000
    ..for which isoAsp formation has been described, namely myrG-D/isoD-AAAAK (deamidated peptide 1-7 of protein kinase A catalytic subunit) and VQ-D/isoD-GLR (deamidated peptide 41-46 of human procollagen alpha 1)...
  79. pmc Addicting drugs utilize a synergistic molecular mechanism in common requiring adenosine and Gi-beta gamma dimers
    Lina Yao
    Ernest Gallo Clinic and Research Center, 5858 Horton Street, Suite 200, Emeryville, CA 94608, USA
    Proc Natl Acad Sci U S A 100:14379-84. 2003
    ..neurons that a brief exposure to saturating concentrations of DOR and CB1 agonists increases cAMP, promotes PKA C alpha translocation and increases cAMP-dependent gene expression...
  80. ncbi Signaling pathways for modulation of mouse sperm motility by adenosine and catecholamine agonists
    Sonya M Schuh
    Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195, USA
    Biol Reprod 74:492-500. 2006
    ..are also absent in sperm from mice lacking the sperm-specific Calpha2 catalytic subunit of protein kinase A (PRKACA). Agonist responses also are strongly suppressed in wild-type sperm by the protein kinase inhibitor H-89...
  81. pmc Genes encoding catalytic subunits of protein kinase A and risk of spina bifida
    Huiping Zhu
    Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, Houston, Texas
    Birth Defects Res A Clin Mol Teratol 73:591-6. 2005
    b>PRKACA and PRKACB are genes encoding the cAMP-dependent protein kinase A (PKA) catalytic subunits alpha and beta, respectively...
  82. doi CDC25B acts as a potential target of PRKACA in fertilized mouse eggs
    Cheng Cui
    Department of Physiology, China Medical University, Shenyang 110001, China
    Biol Reprod 79:991-8. 2008
    Protein kinase A (PRKACA) has been documented as a pivotal regulator in meiosis and mitosis arrest...
  83. pmc Alternate protein kinase A activity identifies a unique population of stromal cells in adult bone
    Kit Man Tsang
    Section on Endocrinology and Genetics, Program on Developmental Endocrinology and Genetics and Pediatric Endocrinology Inter institute Training Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:8683-8. 2010
    ..Prkar1a(+/-) mice were crossed with mice that were heterozygous for catalytic subunit Calpha (Prkaca(+/-)), the main PKA activity-mediating molecule, to generate a mouse model with double heterozygosity for prkar1a ..
  84. doi Expression and biochemical characterization of the Plasmodium falciparum protein kinase A catalytic subunit
    Nathalie Wurtz
    UMR MD3 UR3P, Relation hôte parasites Pharmacologie et Thérapeutique, Institut de Medecine Tropicale du Service de Sante des Armees, Marseille, France
    Parasitol Res 104:1299-305. 2009
    ..This work provides new insights on the study of PfPKAc that will contribute to future investigations of the parasite cAMP signaling pathway and to high-throughput screening of specific malarial PKA inhibitors...
  85. doi Structural analysis of ARC-type inhibitor (ARC-1034) binding to protein kinase A catalytic subunit and rational design of bisubstrate analogue inhibitors of basophilic protein kinases
    Darja Lavogina
    Institute of Chemistry, 2 Jakobi Street, 51014 Tartu, Estonia
    J Med Chem 52:308-21. 2009
    The crystal structure of a complex of the catalytic subunit (type alpha) of cAMP-dependent protein kinase (PKA C alpha) with ARC-type inhibitor (ARC-1034), the presumed lead scaffold of previously reported adenosine-oligo-arginine ..
  86. ncbi Transcriptional regulation of cyclin D2 by the PKA pathway and inducible cAMP early repressor in granulosa cells
    Luis C Muniz
    Department of Biochemistry and Molecular Biology and Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, New Jersey 07103, USA
    Biol Reprod 75:279-88. 2006
    ..The promoter activity of cyclin D2 was shown to be induced by FSH and the catalytic alpha subunit of PKA (PRKACA), and this activity was repressible by inducible cAMP early repressor (ICER), a cAMP response element (CRE) ..
  87. doi Stimulus-induced translocation of the protein kinase A catalytic subunit to the apical membrane in blowfly salivary glands
    Martin Voss
    Institut für Biochemie und Biologie, Universitat Potsdam, Karl Liebknecht Strasse 24 25, 14476 Potsdam, Germany
    Cell Tissue Res 335:657-62. 2009
    ..The recruitment of PKA-C to the apical membrane corroborates our hypothesis that vacuolar H(+)-ATPase, which is enriched in this membrane domain, is a target protein for PKA...
  88. doi Phenotypic and transcriptomic analyses of Plasmodium falciparum protein kinase A catalytic subunit inhibition
    Nathalie Wurtz
    UMR MD3, Relation Hôte Parasites Pharmacologie et Thérapeutique Université de la Méditerranée Aix Marseille II, des Armées Antenne Marseille, Institut de Recherche Biomédicale IMTSSA, Allée du Médecin Colonel Eugène Jamot, Parc du Pharo, Marseille, Cedex 07, France
    Parasitol Res 105:1691-9. 2009
    ....
  89. pmc Epidermal growth factor receptor levels are reduced in mice with targeted disruption of the protein kinase A catalytic subunit
    Morten P Oksvold
    Institute of Pathology, Rikshospitalet University Hospital, University of Oslo, Norway
    BMC Cell Biol 9:16. 2008
    ..Cyclic-AMP-dependent protein kinase consists of a regulatory dimer bound to two catalytic subunits...
  90. ncbi Identification of protein kinase A catalytic subunit beta as a novel binding partner of p73 and regulation of p73 function
    Takayuki Hanamoto
    Division of Biochemistry, Chiba Cancer Center Research Institute, Chiba 260 8717, Japan
    J Biol Chem 280:16665-75. 2005
    ..Using a yeast two-hybrid screening based on the Sos recruitment system, we identified protein kinase A catalytic subunit beta (PKA-Cbeta) as a novel binding partner of p73...
  91. pmc Activity, expression and function of a second Drosophila protein kinase A catalytic subunit gene
    A Melendez
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Genetics 141:1507-20. 1995
    ..These observations indicate that DC2 is not an essential gene and is unlikely to be functionally redundant with DC0, which has multiple unique functions during development...
  92. ncbi Modulation of Drosophila slowpoke calcium-dependent potassium channel activity by bound protein kinase a catalytic subunit
    Yi Zhou
    Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Neurosci 22:3855-63. 2002
    ..These results are consistent with the hypothesis that both binding of active PKAc to dSlo and phosphorylation of dSlo or some other protein are necessary for channel modulation...
  93. pmc Intracellular distribution of mammalian protein kinase A catalytic subunit altered by conserved Asn2 deamidation
    R Pepperkok
    European Molecular Biology Laboratory, D 69012 Heidelberg, Germany
    J Cell Biol 148:715-26. 2000
    ..The model character for other signaling proteins starting with myrGly-Asn is discussed...
  94. ncbi Cloning of a mouse protein kinase A catalytic subunit pseudogene and chromosomal mapping of C subunit isoforms
    D E Cummings
    Department of Pharmacology, University of Washington, Seattle 98195
    Mamm Genome 5:701-6. 1994
    Two isoforms of the protein kinase A catalytic subunit, C alpha and C beta, have previously been described in the mouse. We now report the cloning and characterization of a novel C-related sequence, Cx, from a murine genomic library...
  95. ncbi Androgen-independent growth and tumorigenesis of prostate cancer cells are enhanced by the presence of PKA-differentiated neuroendocrine cells
    Paul D Deeble
    Department of Microbiology and Cancer Center, University of Virginia Health System, Charlottesville, VA 22908, USA
    Cancer Res 67:3663-72. 2007
    ..were engineered to inducibly express a constitutively activated form of the cyclic AMP-dependent protein kinase A catalytic subunit (caPKA), which was previously found upon transient transfection to be sufficient for acquisition of ..
  96. doi Uncoupling of bait-protein expression from the prey protein environment adds versatility for cell and tissue interaction proteomics and reveals a complex of CARP-1 and the PKA Cbeta1 subunit
    Andrea Erlbruch
    Structural Biochemistry, German Cancer Research Center, Heidelberg, Germany
    Proteomics 10:2890-900. 2010
    ..the prey protein environment numerous established, mostly tissue-specific binding partners of the protein kinase A catalytic subunit Cbeta1 were identified, including interactions in binary, ternary and quaternary complexes...
  97. doi Genome-wide gain-of-function screen identifies novel regulators of pluripotency
    Ramzey Abujarour
    Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA
    Stem Cells 28:1487-97. 2010
    ..1/Spi1, Prkaca, and Jun induced differentiation of ES cells...
  98. ncbi Protein phosphatase inhibitor-1 augments a protein kinase A-dependent increase in the Ca2+ loading of the sarcoplasmic reticulum without changing its Ca2+ release
    Hirotaka Kawashima
    Division of Cardiology, Internal Medicine III, Hamamatsu University School of Medicine, Hamamatsu 431 3192, Japan
    Circ J 73:1133-40. 2009
    ..An increase in cytosolic protein phosphatases (PPs) de-phosphorylates phospholamban, decreasing the Ca(2+) uptake of the sarcoplasmic reticulum (SR). The effects of PP inhibitors on cellular Ca(2+) handling were investigated...
  99. doi [Neuroprotective mechanism of tetrahydroxystilbene glucoside on rats after cerebral ischemia-reperfusion]
    Jie Yang
    Department of Neurology, Xiangya Hospital, Central South University, Changsha 410078, China
    Zhong Nan Da Xue Xue Bao Yi Xue Ban 35:321-8. 2010
    ..To investigate the neuroprotective mechanism of tetrahydroxystilbene glucoside (TSG), a Chinese medicine, on rats after cerebral ischemia-reperfusion...
  100. ncbi The role of SF-1/Ad4BP in the control of the bovine gene for the steroidogenic acute regulatory (StAR) protein
    W Rust
    Institute for Hormone and Fertility Research, University of Hamburg, Germany
    J Mol Endocrinol 21:189-200. 1998
    ..Furthermore, additional cotransfection of a construct expressing a constitutive protein kinase A catalytic subunit to mimic the effects of cAMP stimulation, led to a small SF-1/Ad4BP-dependent increase in reporter ..
  101. pmc Activation of the RAS/cyclic AMP pathway suppresses a TOR deficiency in yeast
    Tobias Schmelzle
    Division of Biochemistry, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Mol Cell Biol 24:338-51. 2004
    ..Finally, and importantly, TOR controls the subcellular localization of both the protein kinase A catalytic subunit TPK1 and the RAS/cAMP signaling-related kinase YAK1...

Research Grants71

  1. Mechanisms directing oncoprotein and cytokine mRNA decay
    GERALD WILSON; Fiscal Year: 2007
    ..abstract_text> ..
  2. INSULIN ACTION IN MUSCLE AND FAT CELLS
    James Garrison; Fiscal Year: 2007
    ..Lentivirus will be used to overexpress rictor or to express shRNA to knockdown rictor in 3T3-L1 adipocytes. To investigate mTORC2 function in vivo, we propose to knockout rictor in adipocytes and skeletal muscle of mice. ..
  3. Differentiation in T.cruzi:an emerging AIDS pathogen
    Huan Huang; Fiscal Year: 2007
    ..To further delineate the components of this pathway, we will identify the TcPKA downstream interacting molecules, using both yeast two-hybrid assays and proteomic approaches. ..
  4. Signal Transduction in Depression
    Richard Shelton; Fiscal Year: 2007
    ..These studies may shed light on important cellular mediators of depression and provide new targets for amelioration. ..
  5. CELL SIGNALING REGULATION BY G PROTEIN SUBUNITS
    James Garrison; Fiscal Year: 2005
    ..The approach will be to make mutant beta subunits in which the prenyl group is not able to fold back into the beta subunit and test their activity in assays of receptor interaction and effector function. ..
  6. Mutations in PKA gene transcripts of lupus T cells
    Dama Laxminarayana; Fiscal Year: 2007
    ..abstract_text> ..
  7. Cell Signaling by G Protein betagamma Subunits
    James Garrison; Fiscal Year: 2007
    ..Parameters examined will include: receptor - alpha:betagamma interaction, adenylyl cyclase (cAMP), PLC-beta (Ca2+), Ptdlns 3-kinase (PIP3), P-Rex1 (Rac activation) and cell migration. ..
  8. INSULIN ACTION IN MUSCLE AND FAT CELLS
    JAMES CARLTON GARRISON; Fiscal Year: 2010
    ..Lentivirus will be used to overexpress rictor or to express shRNA to knockdown rictor in 3T3-L1 adipocytes. To investigate mTORC2 function in vivo, we propose to knockout rictor in adipocytes and skeletal muscle of mice. ..
  9. Mechanisms directing oncoprotein and cytokine mRNA decay
    Gerald M Wilson; Fiscal Year: 2010
    ....
  10. Protective role of ANP in the models of acute lung injury
    Anna Birukova; Fiscal Year: 2010
    ....
  11. INSULIN ACTION IN MUSCLE AND FAT CELLS
    JAMES CARLTON GARRISON; Fiscal Year: 2010
    ..Lentivirus will be used to overexpress rictor or to express shRNA to knockdown rictor in 3T3-L1 adipocytes. To investigate mTORC2 function in vivo, we propose to knockout rictor in adipocytes and skeletal muscle of mice. ..
  12. 2/2-Ziprasidone Augmentation of SSRIs for Treatment-Resistant Depression (TRD)
    RICHARD CHARLES SHELTON; Fiscal Year: 2010
    ..We estimate that 180 of these patients will enter the double-blind phase. ..
  13. 2/2-Ziprasidone Augmentation of SSRIs for Treatment-Resistant Depression (TRD)
    Richard Shelton; Fiscal Year: 2009
    ..We estimate that 180 of these patients will enter the double-blind phase. ..
  14. Differentiation in T.cruzi:an emerging AIDS pathogen
    Huan Huang; Fiscal Year: 2009
    ..To further delineate the components of this pathway, we will identify the TcPKA downstream interacting molecules, using both yeast two-hybrid assays and proteomic approaches. ..
  15. Mechanisms directing oncoprotein and cytokine mRNA decay
    GERALD WILSON; Fiscal Year: 2009
    ....
  16. G-protein Regulation of the Phosphatidyl Inositol (3,4,5) Trisphosphate Signal
    James Garrison; Fiscal Year: 2009
    ..abstract_text> ..
  17. HORMONAL CONTROL OF HEPATIC METABOLISM AND FUNCTION
    James Garrison; Fiscal Year: 1993
    ..Success of the reconstitution will be judged by the ability of the G-protein to recouple angiotensin II inhibition of cyclase in toxin treated membranes or to restore high affinity binding in GTP-gamma-S treated membranes...
  18. MOLECULAR NEUROBIOLOGY OF DEPRESSION
    Richard Shelton; Fiscal Year: 2003
    ..Develop further expertise in basic molecular biological methods; 2. Develop new projects oriented to future funding opportunities; and 3. Continue to mentor the next generation of investigators. ..
  19. SEX STEROID REGULATION OF IGF-I EXPRESSION IN BONE
    Thomas McCarthy; Fiscal Year: 2003
    ..Bone mass studies will be conducted using peripheral quantitative computer tomography, and will be correlated with basal and activated skeletal IGF-I expression. ..
  20. VACM-1, cul 5 gene regulates cell growth & angiogenesis.
    Maria Burnatowska Hledin; Fiscal Year: 2004
    ..Consequently, these results may lead to development of strategies for diagnosis and intervention in the pathologies of excessive vascularization and consequently cancer. ..
  21. INSULIN ACTION IN MUSCLE AND FAT CELLS
    James Garrison; Fiscal Year: 2009
    ..Lentivirus will be used to overexpress rictor or to express shRNA to knockdown rictor in 3T3-L1 adipocytes. To investigate mTORC2 function in vivo, we propose to knockout rictor in adipocytes and skeletal muscle of mice. ..
  22. mTOR Signaling Pathways
    James Garrison; Fiscal Year: 2007
    ..We will investigate novel candidate mTOR-interacting proteins identified by MS. Finally, to understand better the mechanisms involved in mTORC2, we propose to identify pianissimo-interacting proteins. ..
  23. G-protein Regulation of the Phosphatidyl Inositol (3,4,5) Trisphosphate Signal
    James Garrison; Fiscal Year: 2007
    ....