Gene Symbol: PPP1R12A
Description: protein phosphatase 1 regulatory subunit 12A
Alias: M130, MBS, MYPT1, protein phosphatase 1 regulatory subunit 12A, myosin binding subunit, myosin phosphatase, target subunit 1, myosin phosphatase-targeting subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12A, protein phosphatase myosin-binding subunit
Species: human
Products:     PPP1R12A

Top Publications

  1. Velasco G, Armstrong C, Morrice N, Frame S, Cohen P. Phosphorylation of the regulatory subunit of smooth muscle protein phosphatase 1M at Thr850 induces its dissociation from myosin. FEBS Lett. 2002;527:101-4 pubmed
    ..Rho kinase is known to control smooth muscle contractility by phosphorylating the 110 kDa myosin-targetting subunit (MYPT1) of the myosin-associated form of protein phosphatase 1 (PP1M)...
  2. Feng J, Ito M, Ichikawa K, Isaka N, Nishikawa M, Hartshorne D, et al. Inhibitory phosphorylation site for Rho-associated kinase on smooth muscle myosin phosphatase. J Biol Chem. 1999;274:37385-90 pubmed
    ..hypothesis that Rho-kinase (Rho-associated kinase) via phosphorylation of the myosin phosphatase target subunit 1 (MYPT1) inhibited MP activity and to identify relevant sites of phosphorylation...
  3. Muranyi A, Zhang R, Liu F, Hirano K, Ito M, Epstein H, et al. Myotonic dystrophy protein kinase phosphorylates the myosin phosphatase targeting subunit and inhibits myosin phosphatase activity. FEBS Lett. 2001;493:80-4 pubmed
    ..An important function of Rho-kinase is to phosphorylate the myosin-binding subunit of myosin phosphatase (MYPT1) and inhibit phosphatase activity. Experiments were carried out to determine if DMPK could function similarly...
  4. Kitazawa T, Eto M, Woodsome T, Khalequzzaman M. Phosphorylation of the myosin phosphatase targeting subunit and CPI-17 during Ca2+ sensitization in rabbit smooth muscle. J Physiol. 2003;546:879-89 pubmed
    ..It has been suggested that phosphorylation at Thr(695) of the MLCP regulatory subunit (MYPT1) and at Thr(38) of the MLCP inhibitor protein CPI-17 results in inhibition of MLCP activity...
  5. Zagorska A, Deak M, Campbell D, Banerjee S, Hirano M, Aizawa S, et al. New roles for the LKB1-NUAK pathway in controlling myosin phosphatase complexes and cell adhesion. Sci Signal. 2010;3:ra25 pubmed publisher
    ..We found that NUAK1 interacts with several myosin phosphatases, including the myosin phosphatase targeting-1 (MYPT1)-protein phosphatase-1beta (PP1beta) complex, through conserved Gly-Ile-Leu-Lys motifs that are direct binding ..
  6. Kimura K, Ito M, Amano M, Chihara K, Fukata Y, Nakafuku M, et al. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science. 1996;273:245-8 pubmed
    ..RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC...
  7. Kiss E, Murányi A, Csortos C, Gergely P, Ito M, Hartshorne D, et al. Integrin-linked kinase phosphorylates the myosin phosphatase target subunit at the inhibitory site in platelet cytoskeleton. Biochem J. 2002;365:79-87 pubmed
    ..composed of the catalytic subunit of type 1 protein phosphatase and myosin phosphatase target subunit isoform 1 (MYPT1) was identified as the major serine/threonine phosphatase component in the platelet-cytoskeleton fraction...
  8. Seko T, Ito M, Kureishi Y, Okamoto R, Moriki N, Onishi K, et al. Activation of RhoA and inhibition of myosin phosphatase as important components in hypertension in vascular smooth muscle. Circ Res. 2003;92:411-8 pubmed
    ..of myosin phosphatase (MP) involved in Ca2+ sensitization of vascular muscle, ie, phosphorylation of either MYPT1, a target subunit of MP or CPI-17, an inhibitory phosphoprotein...
  9. Wilkinson S, Paterson H, Marshall C. Cdc42-MRCK and Rho-ROCK signalling cooperate in myosin phosphorylation and cell invasion. Nat Cell Biol. 2005;7:255-61 pubmed
    ..Our data show that a Cdc42-MRCK signal mediates myosin-dependent cell motility and highlight convergence between Rho and Cdc42 signalling. ..

More Information

Publications129 found, 100 shown here

  1. Wu Y, Murányi A, Erdodi F, Hartshorne D. Localization of myosin phosphatase target subunit and its mutants. J Muscle Res Cell Motil. 2005;26:123-34 pubmed
    Transient transfection of NIH3T3 cells with various constructs of myosin phosphatase target subunit (MYPT1) and GFP showed distinct cellular localizations. Constructs containing the N-terminal nuclear localization signals (NLS), i.e...
  2. Yamashiro S, Yamakita Y, Totsukawa G, Goto H, Kaibuchi K, Ito M, et al. Myosin phosphatase-targeting subunit 1 regulates mitosis by antagonizing polo-like kinase 1. Dev Cell. 2008;14:787-97 pubmed publisher
    Myosin phosphatase-targeting subunit 1 (MYPT1) binds to the catalytic subunit of protein phosphatase 1 (PP1C). This binding is believed to target PP1C to specific substrates including myosin II, thus controlling cellular contractility...
  3. KHROMOV A, Choudhury N, Stevenson A, Somlyo A, Eto M. Phosphorylation-dependent autoinhibition of myosin light chain phosphatase accounts for Ca2+ sensitization force of smooth muscle contraction. J Biol Chem. 2009;284:21569-79 pubmed publisher
    ..underlying the inhibition of MLCP induced by the phosphorylation of myosin phosphatase targeting subunit (MYPT1), a regulatory subunit of MLCP, at Thr-696 and Thr-853 using glutathione S-transferase (GST)-MYPT1 fragments ..
  4. Twomey E, Li Y, Lei J, Sodja C, Ribecco Lutkiewicz M, Smith B, et al. Regulation of MYPT1 stability by the E3 ubiquitin ligase SIAH2. Exp Cell Res. 2010;316:68-77 pubmed publisher
    Myosin phosphatase target subunit 1 (MYPT1), together with catalytic subunit of type1 delta isoform (PP1cdelta) and a small 20-kDa regulatory unit (M20), form a heterotrimeric holoenzyme, myosin phosphatase (MP), which is responsible for ..
  5. Montezano A, De Lucca Camargo L, Persson P, Rios F, Harvey A, Anagnostopoulou A, et al. NADPH Oxidase 5 Is a Pro-Contractile Nox Isoform and a Point of Cross-Talk for Calcium and Redox Signaling-Implications in Vascular Function. J Am Heart Assoc. 2018;7: pubmed publisher
    ..levels, and hyperphosphorylation of pro-contractile signaling molecules MLC20 (myosin light chain 20) and MYPT1 (myosin phosphatase target subunit 1). Blood pressure was similar in wild-type and Nox5 mice...
  6. Liu Z, Zou D, Yang X, Xue X, Zuo L, Zhou Q, et al. Melatonin inhibits colon cancer RKO cell migration by downregulating Rho?associated protein kinase expression via the p38/MAPK signaling pathway. Mol Med Rep. 2017;16:9383-9392 pubmed publisher
    ..In addition, western blot analysis was implemented to examine the expression of ROCK, phospho (p)?myosin phosphatase targeting subunit 1 (MYPT1), p?myosin light chains (MLC) and p?p38...
  7. Zhang J, Zhang D, Wu X, Wang Y, Gu S, Zhu G, et al. Incarvine C suppresses proliferation and vasculogenic mimicry of hepatocellular carcinoma cells via targeting ROCK inhibition. BMC Cancer. 2015;15:814 pubmed publisher
    ..Taken together, the effective inhibitory effect of IVC on MHCC97H cell proliferation and neovascularization was associated with ROCK inhibition, suggesting that IVC may be a new potential drug candidate for the treatment of HCC. ..
  8. Araos P, Mondaca D, Jalil J, Yañez C, Novoa U, Mora I, et al. Diuretics prevent Rho-kinase activation and expression of profibrotic/oxidative genes in the hypertensive aortic wall. Ther Adv Cardiovasc Dis. 2016;10:338-347 pubmed
    ..All treatments significantly reduced blood pressure. In the DOCA rats the p-myosin phosphatase target protein-1 (MYPT1)/t-MYPT1 ratio, index of ROCK activation was higher by 2.8 fold (p < 0.05) compared with control rats...
  9. de Souza P, Guarido K, Scheschowitsch K, da Silva L, Werner M, Assreuy J, et al. Impaired vascular function in sepsis-surviving rats mediated by oxidative stress and Rho-Kinase pathway. Redox Biol. 2016;10:140-147 pubmed publisher
    ..Notably, this is the first study demonstrating long-term dysfunction in the vasculature of sepsis-surviving rats, which take place or remain beyond the acute septic insult. ..
  10. Álvarez Santos M, Carbajal V, Tellez Jiménez O, Martínez Cordero E, Ruiz V, Hernández Pando R, et al. Airway Hyperresponsiveness in Asthma Model Occurs Independently of Secretion of β1 Integrins in Airway Wall and Focal Adhesions Proteins Down Regulation. J Cell Biochem. 2016;117:2385-96 pubmed publisher
    ..The levels of phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr(696) increased in asthma model...
  11. Reho J, Zheng X, Asico L, Fisher S. Redox signaling and splicing dependent change in myosin phosphatase underlie early versus late changes in NO vasodilator reserve in a mouse LPS model of sepsis. Am J Physiol Heart Circ Physiol. 2015;308:H1039-50 pubmed publisher
    ..Contractile (myosin and actin) and regulatory [myosin light chain kinase and phosphatase subunits (Mypt1, CPI-17)] mRNAs and proteins were decreased in mesenteric arteries at 24 h concordant with reduced force ..
  12. El Yazbi A, Abd Elrahman K, Moreno Domínguez A. PKC-mediated cerebral vasoconstriction: Role of myosin light chain phosphorylation versus actin cytoskeleton reorganization. Biochem Pharmacol. 2015;95:263-78 pubmed publisher
    ..Ca(2+), PDBu (i) inhibits MLCP activity via the phosphorylation of myosin targeting subunit of myosin phosphatase (MYPT1) and C-kinase potentiated protein phosphatase-1 inhibitor (CPI-17), (ii) increases the phosphorylation of paxillin ..
  13. Dedinszki D, Kiss A, Márkász L, Márton A, Tóth E, Székely L, et al. Inhibition of protein phosphatase-1 and -2A decreases the chemosensitivity of leukemic cells to chemotherapeutic drugs. Cell Signal. 2015;27:363-72 pubmed publisher
    ..phosphorylation level of the inhibitory sites (Thr696 and Thr853) in the myosin phosphatase (MP) target subunit (MYPT1) as well as in a 25kDa kinase-enhanced phosphatase inhibitor (KEPI)-like protein...
  14. Castañeda P, Muñoz M, García Rojo G, Ulloa J, Bravo J, Márquez R, et al. Association of N-cadherin levels and downstream effectors of Rho GTPases with dendritic spine loss induced by chronic stress in rat hippocampal neurons. J Neurosci Res. 2015;93:1476-91 pubmed publisher
    ..through Rho kinase (ROCK), inactivates myosin phosphatase through phosphorylation of the myosin-binding subunit (MYPT1), producing actomyosin contraction and probable spine loss...
  15. Reho J, Shetty A, Dippold R, Mahurkar A, Fisher S. Unique gene program of rat small resistance mesenteric arteries as revealed by deep RNA sequencing. Physiol Rep. 2015;3: pubmed publisher
    ..Myosin phosphatase regulatory subunit paralogs Mypt1 and p85 were expressed at similar levels, while smooth muscle MLCK was the only such kinase expressed, suggesting ..
  16. Zhang L, Xu Y, Xu J, Wei Y, Xu X. Protein kinase A inhibitor, H89, enhances survival and clonogenicity of dissociated human embryonic stem cells through Rho-associated coiled-coil containing protein kinase (ROCK) inhibition. Hum Reprod. 2016;31:832-43 pubmed publisher
    ..coiled-coil containing protein kinase (ROCK), myosin light chain (MLC2) and myosin phosphatase target subunit 1 (MYPT1), significantly increases cell survival and colony formation, and strongly depresses dissociation-induced cell ..
  17. Akakpo W, Musicki B, Burnett A. cAMP-dependent regulation of RhoA/Rho-kinase attenuates detrusor overactivity in a novel mouse experimental model. BJU Int. 2017;120:143-151 pubmed publisher
    ..Colforsin reverses physiological and molecular abnormalities. This study describes a novel model of detrusor overactivity and suggests a possible basis for its treatment. ..
  18. Chen B, Yang Z, Yang C, Qin W, Gu J, Hu C, et al. A self-organized actomyosin drives multiple intercellular junction disruption and directly promotes neutrophil recruitment in lipopolysaccharide-induced acute lung injury. FASEB J. 2018;:fj201701506RR pubmed publisher
    ..Qin, W., Gu, J., Hu, C., Chen, A., Ning, J., Yi, B., Lu, K. A self-organized actomyosin drives multiple intercellular junction disruption and directly promotes neutrophil recruitment in lipopolysaccharide-induced acute lung injury. ..
  19. Ocaranza M, Fierro C, Jalil J, Moya J, Gonzalez L, Molina C, et al. Rho kinase activation in circulating leukocytes is related to hypertensive myocardial remodeling. Clin Sci (Lond). 2018;132:1837-1853 pubmed publisher
    ..determined in circulating leukocytes measuring phosphorylation of its target myosin phosphatase target subunit 1 (MYPT1), but its relationship with Rho-kinase activity in the myocardium and in vasculature in hypertension has not been ..
  20. Reho J, Kenchegowda D, Asico L, Fisher S. A splice variant of the myosin phosphatase regulatory subunit tunes arterial reactivity and suppresses response to salt loading. Am J Physiol Heart Circ Physiol. 2016;310:H1715-24 pubmed publisher
    ..Variants of MP regulatory subunit Mypt1 are generated by alternative splicing of the 31 nt exon 24 (E24), which, by changing the reading frame, codes for ..
  21. Chiu W, Chiang J, Juang J, Wu C, Tsai C, Tseng Y, et al. Reduction of blood pressure elevation by losartan in spontaneously hypertensive rats through suppression of LARG expression in vascular smooth muscle cells. J Formos Med Assoc. 2019;: pubmed publisher
    ..The study results showed that the reduction of BP elevation by losartan in SHRs occurs through the suppression of LARG expression and MYPT-1 phosphorylation in vascular smooth muscle cells. ..
  22. Horváth D, Sipos A, Major E, Konya Z, Bátori R, Dedinszki D, et al. Myosin phosphatase accelerates cutaneous wound healing by regulating migration and differentiation of epidermal keratinocytes via Akt signaling pathway in human and murine skin. Biochim Biophys Acta Mol Basis Dis. 2018;1864:3268-3280 pubmed publisher
    ..Silencing of, the regulatory subunit of MP (MYPT1 or PPP1R12A), had a negative impact on the migration of keratinocytes and it influenced the cell-cell adhesion properties by ..
  23. Mu S, Liu Y, Jiang J, Ding R, Li X, Li X, et al. Unfractionated heparin ameliorates pulmonary microvascular endothelial barrier dysfunction via microtubule stabilization in acute lung injury. Respir Res. 2018;19:220 pubmed publisher
    ..increase in guanine nucleotide exchange factor (GEF-H1) expression and myosin phosphatase target subunit 1 (MYPT1) phosphorylation, and microtubule (MT) disassembly in LPS-induced ALI mouse model and human pulmonary ..
  24. Park J, Kwon O, Cho S, Cho M, Paick J, Kim S. Comparison of Improving Effects for Diabetic Erectile Dysfunction according to the Anti-Glycemic Agents: Phlorizin and Insulin. World J Mens Health. 2019;37:210-218 pubmed publisher
    ..These results suggest that control of blood glucose level rather than a type of anti-glycemic agents is more important for the prevention and treatment of diabetic erectile dysfunction. ..
  25. Chen D, Lv B, Kobayashi S, Xiong Y, Sun P, Lin Y, et al. Madagascine Induces Vasodilatation via Activation of AMPK. Front Pharmacol. 2016;7:435 pubmed
    ..synthase (eNOS) in endothelial cells while decreasing the phosphorylation of myosin phosphatase target subunit 1 (MYPT1) in VSM cells. Madagascine-induced vasodilatation was abrogated using small interfering RNA knockdown of AMPK...
  26. Rios F, Lopes R, Neves K, Camargo L, Montezano A, Touyz R. Off-Target Vascular Effects of Cholesteryl Ester Transfer Protein Inhibitors Involve Redox-Sensitive and Signal Transducer and Activator of Transcription 3-Dependent Pathways. J Pharmacol Exp Ther. 2016;357:415-22 pubmed publisher
    ..the phosphorylation (2- to 3-fold) of vascular myosin light chain (MLC) and myosin phosphatase target subunit 1 (MYPT1) (procontractile proteins) and stimulated ROS production...
  27. MacDonald J, Sutherland C, Carlson D, Bhaidani S, Al Ghabkari A, Swärd K, et al. A Small Molecule Pyrazolo[3,4-d]Pyrimidinone Inhibitor of Zipper-Interacting Protein Kinase Suppresses Calcium Sensitization of Vascular Smooth Muscle. Mol Pharmacol. 2016;89:105-17 pubmed publisher
    ..myosin phosphatase of 17 kDa (CPI-17), prostate apoptosis response-4, or myosin phosphatase-targeting subunit 1 (MYPT1), all of which have been implicated in the regulation of vascular contractility...
  28. Hedegaard E, Gouliaev A, Winther A, Arcanjo D, Aalling M, Renaltan N, et al. Involvement of Potassium Channels and Calcium-Independent Mechanisms in Hydrogen Sulfide-Induced Relaxation of Rat Mesenteric Small Arteries. J Pharmacol Exp Ther. 2016;356:53-63 pubmed publisher
  29. Xu J, Wang Y, Yang L, Gao Y, Li B, Jin Y. A cytometric assay for ultrasensitive and robust detection of human telomerase RNA based on toehold strand displacement. Biosens Bioelectron. 2017;87:1071-1076 pubmed publisher
    ..Firstly, H1 was immobilized on streptavidin-functionalized magnetic beads (STV-MBs) through biotin-avidin interaction...
  30. Hafidh R, Abdulamir A, Abu Bakar F, Sekawi Z, Jahansheri F, Jalilian F. Novel antiviral activity of mung bean sprouts against respiratory syncytial virus and herpes simplex virus -1: an in vitro study on virally infected Vero and MRC-5 cell lines. BMC Complement Altern Med. 2015;15:179 pubmed publisher
    ..The antiviral activity of methanol extract of mung bean sprouts (MBS), compared to Ribavarin and Acyclovir, on respiratory syncytial virus (RSV) and Herpes Simplex virus -1 (HSV-1) was ..
  31. Kane L, Costa B. Identification of novel allosteric modulator binding sites in NMDA receptors: A molecular modeling study. J Mol Graph Model. 2015;61:204-13 pubmed publisher
    ..The molecular determinants of the modulator binding sites (MBS), analysis of particular MBS electrostatics, and the specific loss or gain of binding after mutations have revealed ..
  32. Hutcheson K, Barrow M, Lisec A, Barringer D, Gries K, Lewin J. What is a clinically relevant difference in MDADI scores between groups of head and neck cancer patients?. Laryngoscope. 2016;126:1108-13 pubmed publisher
    ..Retrospective cross-sectional study was conducted in 1,136 HNC patients seen for modified barium swallow (MBS) studies. The MDADI was administered by written questionnaire at the MBS appointment...
  33. Li Y, Xu J, Chen Y, Mei Z, Xiao Y. Screening of inhibitors of glycogen synthase kinase-3β from traditional Chinese medicines using enzyme-immobilized magnetic beads combined with high-performance liquid chromatography. J Chromatogr A. 2015;1425:8-16 pubmed publisher
    Glycogen synthase kinase-3β (GSK-3β) was immobilized on magnetic beads (MBs) by affinity method for the first time...
  34. Chen Y, Ibragimov U, Nehl E, Zheng T, He N, Wong F. Validity of the CAGE questionnaire for men who have sex with men (MSM) in China. Drug Alcohol Depend. 2016;160:151-6 pubmed publisher
    ..waves of cross-sectional assessments among general MSM (n=523) and men who sell sex to other men ("money boys" or MBs, n=486) in Shanghai, China...
  35. Li F, Zhao H, Wang Z, Wu Z, Yang Z, Li C, et al. Single palindromic molecular beacon-based amplification for genetic analysis of cancers. Biosens Bioelectron. 2017;91:692-698 pubmed publisher
    ..Upon specific hybridization with target DNA, P-MBs can interact with each other and are extended by polymerase without any additional probes...
  36. Wang Y, Yang L, Wang Y, Liu W, Li B, Jin Y. An ultra-sensitive colorimetric assay for reliable visual detection of telomerase activity. Analyst. 2017;142:3235-3240 pubmed publisher
    ..A telomerase substrate (TS) primer was immobilized onto magnetic beads (MBs) to form a MB/TS complex...
  37. Musso P, Lampin Saint Amaux A, Tchénio P, Preat T. Ingestion of artificial sweeteners leads to caloric frustration memory in Drosophila. Nat Commun. 2017;8:1803 pubmed publisher
    ..CFM formation involves activity of the associative memory brain structure, the mushroom bodies (MBs)...
  38. Napoli C, Lai S, Giannetti A, Tombelli S, Baldini F, Barbaro M, et al. Electronic Detection of DNA Hybridization by Coupling Organic Field-Effect Transistor-Based Sensors and Hairpin-Shaped Probes. Sensors (Basel). 2018;18: pubmed publisher
    ..properties in terms of sensitivity and selectivity in other kinds of assays, in the form of molecular beacons (MBs)...
  39. Cho H, Suzuki T, Dohmae N, Hayami S, Unoki M, Yoshimatsu M, et al. Demethylation of RB regulator MYPT1 by histone demethylase LSD1 promotes cell cycle progression in cancer cells. Cancer Res. 2011;71:655-60 pubmed publisher
    ..In this study, we explored hypothesized interactions between LSD1 and MYPT1, a known regulator of RB1 phosphorylation...
  40. Weiss K, Applegate C, Wang T, Batista D. Familial TAB2 microdeletion and congenital heart defects including unusual valve dysplasia and tetralogy of fallot. Am J Med Genet A. 2015;167A:2702-6 pubmed publisher
    ..Reported cases have genomic deletions of 2-10 Mbs including TAB2 at 6q24-25 are almost always de novo and show variable cardiac and extra cardiac phenotype...
  41. Pei H, Zhu S, Yang M, Kong R, Zheng Y, Qu F. Graphene oxide quantum dots@silver core-shell nanocrystals as turn-on fluorescent nanoprobe for ultrasensitive detection of prostate specific antigen. Biosens Bioelectron. 2015;74:909-14 pubmed publisher
    ..In particular, magnetic beads (MBs) were employed to immobilize anti-PSA antibody (Ab1) and acted as a separable capture probe, while GQDs@Ag was used ..
  42. Engholm M, Pinilla E, Mogensen S, Matchkov V, Hedegaard E, Chen H, et al. Involvement of transglutaminase 2 and voltage-gated potassium channels in cystamine vasodilatation in rat mesenteric small arteries. Br J Pharmacol. 2016;173:839-55 pubmed publisher
    ..tension, intracellular Ca(2+) ([Ca(2+)]i ), K(+) currents (patch clamp), and phosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) and myosin regulatory light chain, in our experiments...
  43. Biswas G, Dutta M, Dutta S, Adhikari K. A comparative study of removal of fluoride from contaminated water using shale collected from different coal mines in India. Environ Sci Pollut Res Int. 2016;23:9418-31 pubmed publisher
    ..Shale samples were collected from Mahabir colliery (MBS) and Sonepur Bazari colliery (SBS) of Raniganj coalfield in West Bengal, India, and used to remove fluoride...
  44. Volpe G, Delibato E, Fabiani L, Pucci E, Piermarini S, D Angelo A, et al. Development and evaluation of an ELIME assay to reveal the presence of Salmonella in irrigation water: Comparison with Real-Time PCR and the Standard Culture Method. Talanta. 2016;149:202-210 pubmed publisher
    ..Magnetic beads (MBs), coupled to a strip of eight-magnetized screen-printed electrodes localized at the bottom of eight wells (8-well/..
  45. Chen M, Hao Y, Qiu M, Song J, Wu D, Humar I. Mobility-Aware Caching and Computation Offloading in 5G Ultra-Dense Cellular Networks. Sensors (Basel). 2016;16: pubmed publisher
    ..local caching, Device-to-Device (D2D) caching, Small cell Base Station (SBS) caching and Macrocell Base Station (MBS) caching. However, studies show that so far, much of the research has ignored the impact of user mobility...
  46. van Rooij T, Skachkov I, Beekers I, Lattwein K, Voorneveld J, Kokhuis T, et al. Viability of endothelial cells after ultrasound-mediated sonoporation: Influence of targeting, oscillation, and displacement of microbubbles. J Control Release. 2016;238:197-211 pubmed publisher
    Microbubbles (MBs) have been shown to create transient or lethal pores in cell membranes under the influence of ultrasound, known as ultrasound-mediated sonoporation...
  47. Shichi D, Arimura T, Ishikawa T, Kimura A. Heart-specific small subunit of myosin light chain phosphatase activates rho-associated kinase and regulates phosphorylation of myosin phosphatase target subunit 1. J Biol Chem. 2010;285:33680-90 pubmed publisher
    ..MLCP consists of a catalytic subunit, a large subunit (MYPT1 or MYPT2), and a small subunit...
  48. Jin X, Guo B, Yan J, Yang R, Chang L, Wang Y, et al. Angiotensin II increases secreted frizzled-related protein 5 (sFRP5) expression through AT1 receptor/Rho/ROCK1/JNK signaling in cardiomyocytes. Mol Cell Biochem. 2015;408:215-22 pubmed publisher
    ..RT-PCR and Western-blot determined the expressions of sFRP5. BNP, TNF-α, ROCK1, ROCK2, MYPT1, and JNK were examined through Western-blot analysis...
  49. Bertol J, Gatti M. Rapid detection of infectious rotavirus group A using a molecular beacon assay. J Virol Methods. 2016;234:156-9 pubmed publisher
    ..Molecular beacons (MBs) have demonstrated efficacy for viral detection in cell culture...
  50. Kim J, Go Y, Kim J, Chung B. Characterization of microRNAs and their target genes associated with transcriptomic changes in gamma-irradiated Arabidopsis. Genet Mol Res. 2016;15: pubmed publisher
    ..These eight miRNA genes contain UV-B-responsive cis-elements, including G-box, I-box core, ARE, and/or MBS in the putative promoter regions...
  51. Kumar A, D Souza S, Moskvin O, Toh H, Wang B, Zhang J, et al. Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts. Cell Rep. 2017;19:1902-1916 pubmed publisher
    ..In clonogenic cultures, MBs differentiate into primitive PDGFRβ+CD271+CD73- mesenchymal progenitors, which ..
  52. Zhao Y, Zhang Q, Xi J, Xiao B, Li Y, Ma C. Neuroprotective effect of fasudil on inflammation through PI3K/Akt and Wnt/β-catenin dependent pathways in a mice model of Parkinson's disease. Int J Clin Exp Pathol. 2015;8:2354-64 pubmed
    ..Expression of tyrosine hydroxylase (TH), p-MYPT1, p-nuclear transcription factor NF-κB, toll-like receptor 2 (TLR2), arginase1, inducible nitric oxide synthase (..
  53. Hassani Mahmooei B, Berecki Gisolf J, Hahn Y, McClure R. The effect of pre-existing health conditions on the cost of recovery from road traffic injury: insights from data linkage of medicare and compensable injury claims in Victoria, Australia. BMC Health Serv Res. 2016;16:162 pubmed publisher
    ..Australia and who provided consent for Pharmaceutical Benefits Scheme (PBS) and Medicare Benefits Schedule (MBS) linkage were included (n?=?738) in the analysis...
  54. Hsieh S, Wu C, Hsu S, Feng C, Yen J. Gallic acid attenuates TGF-?1-stimulated collagen gel contraction via suppression of RhoA/Rho-kinase pathway in hypertrophic scar fibroblasts. Life Sci. 2016;161:19-26 pubmed publisher
    ..challenge and GA insult were evaluated using RhoA-G-LISA and RhoA-ELISA kit while the phosphorylation level of MYPT1 and the expression of ROCK1 and ROCK2 were examined by western blot, respectively...
  55. Day K, Smith N, Kulbersh B. Early surgical intervention in type I laryngeal cleft. Int J Pediatr Otorhinolaryngol. 2016;90:236-240 pubmed publisher
    ..e. absence of previous symptoms) per parental report in follow-up visits, +/- normal post-operative MBS (modified barium swallow) findings, was seen in 11 patients (61%)...
  56. Li X, Xu X, Song J, Xue Q, Li C, Jiang W. Sensitive detection of T4 polynucleotide kinase activity based on multifunctional magnetic probes and polymerization nicking reactions mediated hyperbranched rolling circle amplification. Biosens Bioelectron. 2017;91:631-636 pubmed publisher
    ..First, the streptavidin-magnetic nanobeads (MBs) were functionalized with the biotin modified hairpin probe (HP) with 3'-phosphoryl, forming multifunctional ..
  57. Gordon D, Zelaya A, Ronk K, Traniello J. Interspecific comparison of mushroom body synaptic complexes of dimorphic workers in the ant genus Pheidole. Neurosci Lett. 2018;662:110-114 pubmed publisher
    ..Results support the hypothesis that MG density in the lip (olfactory) and collar (visual) regions of the MBs decrease with age in association with increases in bouton size in the lip...
  58. Wei L, Murphy B, Wu G, Parker M, Easton J, Gilbertson R, et al. Exome sequencing analysis of murine medulloblastoma models identifies WDR11 as a potential tumor suppressor in Group 3 tumors. Oncotarget. 2017;8:64685-64697 pubmed publisher
    ..After filtering and cross-species analysis with 366 human MBs from four independent studies, human orthologs for 16 of the 40 mouse genes were found to harbor non-silent ..
  59. Wu S, Gan G, Zhang Z, Sun J, Wang Q, Gao Z, et al. A Presynaptic Function of Shank Protein in Drosophila. J Neurosci. 2017;37:11592-11604 pubmed publisher
    ..Our results demonstrate that, in addition to its conventional postsynaptic function, Shank also acts presynaptically in synapse development in the brain. This study offers novel insights into the synaptic role of Shank. ..
  60. Arienzo A, Sobze M, Wadoum R, Losito F, Colizzi V, Antonini G. Field Application of the Micro Biological Survey Method for a Simple and Effective Assessment of the Microbiological Quality of Water Sources in Developing Countries. Int J Environ Res Public Health. 2015;12:10314-28 pubmed publisher
    ..The aim of this work was to apply an alternative method, the Micro Biological Survey (MBS), to analyse for contaminants in drinking water...
  61. Galinato M, Fogle R, Stetz A, Galan J. Modulating the nitrite reductase activity of globins by varying the heme substituents: Utilizing myoglobin as a model system. J Inorg Biochem. 2016;154:7-20 pubmed publisher
    ..This is accomplished by studying Mbs with hemes that have different 2,4-R groups, namely diacetyldeuteroMb (-acetyl), protoMb (wild-type (wt) Mb, -vinyl)..
  62. Li Y, Jiang T, Shao L, Liu Y, Zheng C, Zhong Y, et al. Mir-449a, a potential diagnostic biomarker for WNT group of medulloblastoma. J Neurooncol. 2016;129:423-431 pubmed publisher
    ..current study demonstrates for the first time the feasibility of using the Nanostring assay for subgrouping of MBs in Chinese patients...
  63. Yang S, Wu Q, Huang S, Wang Z, Qi F. Sevoflurane and isoflurane inhibit KCl-induced Class II phosphoinositide 3-kinase ? subunit mediated vasoconstriction in rat aorta. BMC Anesthesiol. 2016;16:63 pubmed publisher
    ..inhibitor Y27632, and membrane translocation of PI3K-p85, PI3K-C2?, Rho kinase (Rock II), or phosphorylation of MYPT1/Thr853, MYPT1/Thr696, CPI-17/Thr38 and MLC in response to KCl (60 mM) was measured by using isometric force ..
  64. Boyd J, Pargaonkar V, Scoville D, Rogers I, Kimura T, Tanaka S, et al. Surgical Unroofing of Hemodynamically Significant Left Anterior Descending Myocardial Bridges. Ann Thorac Surg. 2017;103:1443-1450 pubmed publisher
    Left anterior descending artery myocardial bridges (MBs) range from clinically insignificant incidental angiographic findings to a potential cause of sudden cardiac death...
  65. Ueno K, Suzuki E, Naganos S, Ofusa K, Horiuchi J, Saitoe M. Coincident postsynaptic activity gates presynaptic dopamine release to induce plasticity in Drosophila mushroom bodies. elife. 2017;6: pubmed publisher
    ..lobes (ALs) and the ascending fibers of the ventral nerve cord (AFV), two sensory inputs to the mushroom bodies (MBs), induces long-term enhancement (LTE) of subsequent AL-evoked MB responses...
  66. Lin L, Xu C, Carraway M, Piantadosi C, Whorton A, Li S. RhoA inactivation by S-nitrosylation regulates vascular smooth muscle contractive signaling. Nitric Oxide. 2018;74:56-64 pubmed publisher
    ..In both smooth muscle cells and aortic tissue, the outcome was inhibition of agonist-stimulated MYPT1 phosphorylation and reduced levels of myosin light chain phosphorylation...
  67. Yin L, Xu Y, Peng L, Duan T, Liu J, Xu Z, et al. Transgelin-2 as a therapeutic target for asthmatic pulmonary resistance. Sci Transl Med. 2018;10: pubmed publisher
    ..relaxes ASMCs via transgelin-2 (TG2) and induces dephosphorylation of myosin phosphatase target subunit 1 (MYPT1)...
  68. Chao A, Zhang X, Ma D, Langlais P, Luo M, Mandarino L, et al. Site-specific phosphorylation of protein phosphatase 1 regulatory subunit 12A stimulated or suppressed by insulin. J Proteomics. 2012;75:3342-50 pubmed publisher
    ..So far, only few specific phosphorylation sites of PP1 regulatory subunit 12A (PPP1R12A) have been shown to regulate the PP1 activity...
  69. Gomes J, Gomes Alves P, Carvalho S, Peixoto C, Alves P, Altevogt P, et al. Extracellular Vesicles from Ovarian Carcinoma Cells Display Specific Glycosignatures. Biomolecules. 2015;5:1741-61 pubmed publisher
    ..Here we have isolated and characterized extracellular vesicles (EVs) and total cell membranes (MBs) from ovarian carcinoma OVMz cells...
  70. Ruiz Valdepeñas Montiel V, Campuzano S, Torrente Rodríguez R, Reviejo A, Pingarron J. Electrochemical magnetic beads-based immunosensing platform for the determination of ?-lactalbumin in milk. Food Chem. 2016;213:595-601 pubmed publisher
    ..In this work, we present, for the first time, a very sensitive magnetic beads (MBs)-based immunosensor for the determination of ?-LA...
  71. Seugnet L, Dissel S, Thimgan M, Cao L, Shaw P. Identification of Genes that Maintain Behavioral and Structural Plasticity during Sleep Loss. Front Neural Circuits. 2017;11:79 pubmed publisher
    ..Surprisingly, decreasing hiw and Rdl within the Mushroom Bodies (MBs) protects against the negative effects of sleep deprivation (SD) as indicated by the absence of a subsequent ..
  72. Yang Y, Chen M, Krueger C, Tsourkas A, Chen A. Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons. Methods Mol Biol. 2018;1649:231-242 pubmed publisher
    Molecular beacons (MBs), a class of oligonucleotide-based probes, have enabled researchers to study various RNA molecules in their native live-cell contexts...
  73. Magwanga R, Lu P, Kirungu J, Lu H, Wang X, Cai X, et al. Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton. BMC Genet. 2018;19:6 pubmed publisher
    ..Almost all the LEA genes in their promoters contained ABRE, MBS, W-Box and TAC-elements, functionally known to be involved in drought stress and other stress responses...
  74. Currie J, Chiarella M, Buckley T. Collaborative arrangements and privately practising nurse practitioners in Australia: results from a national survey. Aust Health Rev. 2017;41:533-540 pubmed publisher
    ..practitioners (PPNPs) in Australia have been eligible for subsidisation through the Medicare Benefits Schedule (MBS) and Pharmaceutical Benefits Scheme (PBS)...
  75. Xu J, Zheng T, Le J, Jia L. Long-stem shaped multifunctional molecular beacon for highly sensitive nucleic acids determination via intramolecular and intermolecular interactions based strand displacement amplification. Analyst. 2017;142:4438-4445 pubmed publisher
    ..the hybridization event synchronously, which is distinctly advantageous over the traditional molecular beacons (MBs)...
  76. Ahmed M, Cerroni B, Razuvaev A, Härmark J, Paradossi G, Caidahl K, et al. Cellular Uptake of Plain and SPION-Modified Microbubbles for Potential Use in Molecular Imaging. Cell Mol Bioeng. 2017;10:537-548 pubmed publisher
    ..For US gas-filled microbubbles (MBs) or silica nanoparticles (SiNPs), and for MRI superparamagnetic or paramagnetic agents, contribute to this...
  77. Mulder J, Ariaens A, van Horck F, Moolenaar W. Inhibition of RhoA-mediated SRF activation by p116Rip. FEBS Lett. 2005;579:6121-7 pubmed
    ..partner of activated RhoA, is an actin-binding protein that interacts with the regulatory myosin-binding subunit (MBS) of myosin-II phosphatase and is essential for Rho-regulated cytoskeletal contractility...
  78. Waragai M, Nagamitsu S, Xu W, Li Y, Lin X, Ashizawa T. Ataxin 10 induces neuritogenesis via interaction with G-protein beta2 subunit. J Neurosci Res. 2006;83:1170-8 pubmed
    ..Our data suggest that the ataxin 10-Gbeta2 interaction represents a novel mechanism for inducing neuritogenesis in PC12 cells by activating the Ras-MAP kinase-Elk-1 cascade. ..
  79. Webb J, Murányi A, Pugh C, Ratcliffe P, Coleman M. MYPT1, the targeting subunit of smooth-muscle myosin phosphatase, is a substrate for the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH). Biochem J. 2009;420:327-33 pubmed publisher
    ..hydroxylation in a bioinformatically predicted FIH substrate, the targeting subunit of myosin phosphatase, MYPT1. Our results confirm hydroxylation both in cultured cells and in endogenous protein purified from animal tissue...
  80. Lontay B, Bodoor K, Weitzel D, Loiselle D, Fortner C, Lengyel S, et al. Smoothelin-like 1 protein regulates myosin phosphatase-targeting subunit 1 expression during sexual development and pregnancy. J Biol Chem. 2010;285:29357-66 pubmed publisher
    ..A primary target gene of SMTNL1 in these muscles is myosin phosphatase-targeting subunit 1 (MYPT1)...
  81. Lin Z, Chen G, Zhang Y, He H, Liang Y, Ye J, et al. MicroRNA-30d promotes angiogenesis and tumor growth via MYPT1/c-JUN/VEGFA pathway and predicts aggressive outcome in prostate cancer. Mol Cancer. 2017;16:48 pubmed publisher
    ..Simulation of myosin phosphatase targeting subunit 1 (MYPT1), acting as a direct target of miR-30d, antagonized the effects induced by miR-30d up-..
  82. Hou Y, Wang J, Jiang Y, Lv C, Xia L, Hong S, et al. A colorimetric and electrochemical immunosensor for point-of-care detection of enterovirus 71. Biosens Bioelectron. 2018;99:186-192 pubmed publisher
    ..The dual-labeled magnetic nanobeads (DL-MBs) are fabricated by simultaneous immobilization of EV71 monoclonal antibody (mAb) and horseradish peroxidase (HRP) ..
  83. Singh J, Mohanty I, Rattan S. In vivo magnetofection: a novel approach for targeted topical delivery of nucleic acids for rectoanal motility disorders. Am J Physiol Gastrointest Liver Physiol. 2017;:ajpgi.00233.2017 pubmed publisher
    ..Present studies demonstrate that magnetofection-mediated miR-139-5p delivery significantly decreased RhoA/ROCK2, p-MYPT1, and p-MLC20 signaling, leading to decreases in the lower basal IASP and IAS tone and in rates of contraction and ..
  84. Wang X, Amei A, de Belle J, Roberts S. Environmental effects on Drosophila brain development and learning. J Exp Biol. 2018;221: pubmed publisher
    ..associative learning in adult Drosophila melanogaster In response to heat stress, the mushroom bodies (MBs) were the most volumetrically impaired among all of the brain structures, an effect highly correlated with reduced ..
  85. Rohith B, Shyamala B. Scalloped a member of the Hippo tumor suppressor pathway controls mushroom body size in Drosophila brain by non-canonical regulation of neuroblast proliferation. Dev Biol. 2017;432:203-214 pubmed publisher
    ..Here we have studied the hypomorphic effect of sd on the development of Mushroom Bodies (MBs) in Drosophila brain. We show that, sd non-function results in an increase in the size of MBs...
  86. Yang X, Yang K, Zhao X, Lin Z, Liu Z, Luo S, et al. Terahertz spectroscopy for the isothermal detection of bacterial DNA by magnetic bead-based rolling circle amplification. Analyst. 2017;142:4661-4669 pubmed publisher
    ..a circular PLP upon recognition of the target sequence; then the capture probe (CP) immobilized on magnetic beads (MBs) acted as a primer to initialize RCA...
  87. Yang L, Dai F, Tang L, Le Y, Yao W. Macrophage differentiation induced by PMA is mediated by activation of RhoA/ROCK signaling. J Toxicol Sci. 2017;42:763-771 pubmed publisher
    ..Western blot analysis showed that the expression of ROCK1 and ROCK2 and the phosphorylation of MYPT1 were significantly increased after PMA treatment...
  88. Kucharski R, Ball E, Hayward D, Maleszka R. Molecular cloning and expression analysis of a cDNA encoding a glutamate transporter in the honeybee brain. Gene. 2000;242:399-405 pubmed
    ..brain, and its transcripts are abundant in the optic lobes and inner compact Kenyon cells of the mushroom bodies (MBs), with most other regions of the brain showing lower levels of Am-EAAT expression...
  89. Ficarro S, Salomon A, Brill L, Mason D, Stettler Gill M, Brock A, et al. Automated immobilized metal affinity chromatography/nano-liquid chromatography/electrospray ionization mass spectrometry platform for profiling protein phosphorylation sites. Rapid Commun Mass Spectrom. 2005;19:57-71 pubmed
  90. Parra M, Mahmoudi T, Verdin E. Myosin phosphatase dephosphorylates HDAC7, controls its nucleocytoplasmic shuttling, and inhibits apoptosis in thymocytes. Genes Dev. 2007;21:638-43 pubmed
    ..Here, we identify protein phosphatase 1beta (PP1beta) and myosin phosphatase targeting subunit 1 (MYPT1), two components of the myosin phosphatase complex, as HDAC7-associated proteins in ..
  91. Iwasaki T, Katayama T, Kohama K, Endo Y, Sawasaki T. Myosin phosphatase is inactivated by caspase-3 cleavage and phosphorylation of myosin phosphatase targeting subunit 1 during apoptosis. Mol Biol Cell. 2013;24:748-56 pubmed publisher
    ..In this study, we have demonstrated that, in apoptotic cells, the myosin-binding domain of myosin phosphatase targeting subunit 1 (MYPT1) is cleaved by caspase-3 at Asp-884, and the cleaved MYPT1 is strongly phosphorylated at ..
  92. Takeya K, Kaneko T, Miyazu M, Takai A. Addition of urea and thiourea to electrophoresis sample buffer improves efficiency of protein extraction from TCA/acetone-treated smooth muscle tissues for phos-tag SDS-PAGE. Electrophoresis. 2017;: pubmed publisher
    ..Standard SDS sample buffer extracted less LC20 , actin and myosin phosphatase targeting subunit 1 (MYPT1) from TCA/acetone treated ciliary muscle strips...