Gene Symbol: POU4F1
Description: POU class 4 homeobox 1
Alias: BRN3A, Oct-T1, RDC-1, brn-3A, POU domain, class 4, transcription factor 1, brain-3A, brain-specific homeobox/POU domain protein 3A, homeobox/POU domain protein RDC-1
Species: human
Products:     POU4F1

Top Publications

  1. Collum R, Fisher P, Datta M, Mellis S, Thiele C, Huebner K, et al. A novel POU homeodomain gene specifically expressed in cells of the developing mammalian nervous system. Nucleic Acids Res. 1992;20:4919-25 pubmed
    ..Based on its unique structural characteristics and expression pattern, we discuss potential functions for the RDC-1 protein. ..
  2. Xiang M, Zhou L, Macke J, Yoshioka T, Hendry S, Eddy R, et al. The Brn-3 family of POU-domain factors: primary structure, binding specificity, and expression in subsets of retinal ganglion cells and somatosensory neurons. J Neurosci. 1995;15:4762-85 pubmed
  3. Gerrero M, McEvilly R, Turner E, Lin C, O Connell S, Jenne K, et al. Brn-3.0: a POU-domain protein expressed in the sensory, immune, and endocrine systems that functions on elements distinct from known octamer motifs. Proc Natl Acad Sci U S A. 1993;90:10841-5 pubmed
    ..Brn-3.0, Brn-3.1, and the Drosophila tI-POU share an N-terminal region of homology, referred to as the "POU-IV box," which is similar to a conserved functional domain in the c-myc gene family. ..
  4. Ma L, Lei L, Eng S, Turner E, Parada L. Brn3a regulation of TrkA/NGF receptor expression in developing sensory neurons. Development. 2003;130:3525-34 pubmed
    ..The molecular mechanisms regulating tissue and stage-specific expression of TrkA are largely unknown. The Brn3a POU-domain transcription factor has been implicated in the development of the PNS and proposed as a transcription ..
  5. Smith M, Dawson S, Latchman D. Inhibition of neuronal process outgrowth and neuronal specific gene activation by the Brn-3b transcription factor. J Biol Chem. 1997;272:1382-8 pubmed
    ..Hence a single amino acid difference results in these closely related factors having opposite effects and allows the balance between them to regulate differentiation. ..
  6. Trieu M, Ma A, Eng S, Fedtsova N, Turner E. Direct autoregulation and gene dosage compensation by POU-domain transcription factor Brn3a. Development. 2003;130:111-21 pubmed
    b>Brn3a is a POU-domain transcription factor expressed in peripheral sensory neurons and in specific interneurons of the caudal CNS...
  7. Calissano M, Latchman D. Functional interaction between the small GTP-binding protein Rin and the N-terminal of Brn-3a transcription factor. Oncogene. 2003;22:5408-14 pubmed
    ..In this work, we describe the evidence for a functional interaction between Brn-3a and Rin and demonstrate the role of Rin in modulating the activation of the Brn-3a regulated egr-1 promoter by the N-terminal domain of Brn-3a. ..
  8. Diss J, Faulkes D, Walker M, Patel A, Foster C, Budhram Mahadeo V, et al. Brn-3a neuronal transcription factor functional expression in human prostate cancer. Prostate Cancer Prostatic Dis. 2006;9:83-91 pubmed
    ..7 sodium channel, concomitantly upregulated in human CaP, in an isoform-specific manner. It is concluded that targeting Brn-3a could be a useful strategy for controlling the expression of multiple genes that promote CaP. ..
  9. Smith A, Rohrer B, Wheless L, Samantaray S, Ray S, Inoue J, et al. Calpain inhibition reduces structural and functional impairment of retinal ganglion cells in experimental optic neuritis. J Neurochem. 2016;139:270-284 pubmed publisher
    ..oral treatment with the calpain inhibitor SNJ 1945 preserves visual acuity and preserves retinal ganglion cells (Brn3a, brain-specific homeobox/POU domain protein 3A) and their axons (MOSP, myelin oligodendrocyte-specific protein)...

More Information

Publications128 found, 100 shown here

  1. Xu Z, Fouda A, Lemtalsi T, Shosha E, Rojas M, Liu F, et al. Retinal Neuroprotection From Optic Nerve Trauma by Deletion of Arginase 2. Front Neurosci. 2018;12:970 pubmed publisher
    ..These analyses showed increased A2 expression following ONC. Numbers of NeuN-positive neurons as well as Brn3a- and RBPMS-positive RGC were decreased in the WT retinas at 14 days after ONC as compared to the sham controls...
  2. Kitamura Y, Bikbova G, Baba T, Yamamoto S, Oshitari T. In vivo effects of single or combined topical neuroprotective and regenerative agents on degeneration of retinal ganglion cells in rat optic nerve crush model. Sci Rep. 2019;9:101 pubmed publisher
    ..After 2 weeks, the number of RGCs was determined by Brn3a immunostaining...
  3. Bartesaghi L, Wang Y, Fontanet P, Wanderoy S, Berger F, Wu H, et al. PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis. Cell Rep. 2019;26:3484-3492.e4 pubmed publisher
    ..of neurogenesis and activation of a cascade of downstream pro-neuronal transcription factors, including NEUROD1, BRN3A, and ISL1, in the nociceptive lineage while it represses alternative fates other than nociceptors in progenitor ..
  4. Rovere G, Nadal Nicolás F, Sobrado Calvo P, Villegas Pérez M, Vidal Sanz M, Agudo Barriuso M. Topical bromfenac transiently delays axotomy-induced retinal ganglion cell loss. Exp Eye Res. 2019;: pubmed publisher
    ..Intact untreated retinas were used as control of the RGC population. RGCs were identified by Brn3a immunodetection and automatically quantified...
  5. Zhang X, Piano I, Messina A, D Antongiovanni V, Crò F, Provenzano G, et al. Retinal defects in mice lacking the autism-associated gene Engrailed-2. Neuroscience. 2019;: pubmed publisher
    ..The total number of ganglion cells was not altered in the adult En2-/- retina, as shown by Brn3a+ cell counts...
  6. Bao Y, Liu F, Liu X, Huang M, He L, Ramakrishna S, et al. Methyl 3,4-dihydroxybenzoate protects retina in a mouse model of acute ocular hypertension through multiple pathways. Exp Eye Res. 2019;181:15-24 pubmed publisher
    ..Results showed that AOH injury reduced the number of Brn3a-stained retinal ganglion cells (RGCs) and ChAT-amacrine cells; thinned the inner retinal layers and induced ..
  7. Tsoka P, Barbisan P, Kataoka K, Chen X, Tian B, Bouzika P, et al. NLRP3 inflammasome in NMDA-induced retinal excitotoxicity. Exp Eye Res. 2019;181:136-144 pubmed publisher
    ..Similarly, the deletion of the two aforementioned genes did not alter the survival of the Brn3a+ (brain-specific homeobox/POU domain protein 3A) RGCs in a significant way at 3- or 7-days post ..
  8. Lobanovskaya N, Zharkovsky A. A role of PSA-NCAM in the survival of retinal ganglion cells (RGCs) after kainic acid damage. Neurotoxicology. 2019;72:101-106 pubmed publisher
    ..KA was injected intravitreally to induce RGC damage. RGCs were visualized using an anti-Brn3a antibody. Endoneuraminidase N (NA) was administrated intravitreally to cleave PSA chains from NCAM...
  9. Wang S, Chen L, Zhang P, Wang X, Sun Y, Ma L, et al. Transplantation of retinal progenitor cells from optic cup-like structures differentiated from human embryonic stem cells in vitro and in vivo generation of retinal ganglion-like cells. Stem Cells Dev. 2018;: pubmed publisher
    ..Surprisingly, some of the transplanted cells expressed the RGC-specific marker Brn3a. These findings demonstrated that the RPCs derived from hESCs could integrate into the host GCL and differentiate ..
  10. Pan D, Chang X, Xu M, Zhang M, Zhang S, Wang Y, et al. UMSC-derived exosomes promote retinal ganglion cells survival in a rat model of optic nerve crush. J Chem Neuroanat. 2019;96:134-139 pubmed publisher
    ..After three times of treatments with an interval of one week, we found that the UMSC-Exos significantly promoted Brn3a+ retinal ganglion cells (RGCs) survival in retinal ganglion cell layer compared with PBS controls...
  11. Rovere G, Nadal Nicolás F, Wang J, Bernal Garro J, García Carrillo N, Villegas Pérez M, et al. Melanopsin-Containing or Non-Melanopsin-Containing Retinal Ganglion Cells Response to Acute Ocular Hypertension With or Without Brain-Derived Neurotrophic Factor Neuroprotection. Invest Ophthalmol Vis Sci. 2016;57:6652-6661 pubmed publisher
    ..were dissected as wholemounts and immunolabeled for melanopsin (to identify intrinsically photosensitive RGCs) or Brn3a (to identify all RGCs except melanopsin +RGCs)...
  12. Zalis M, Johansson S, Englund Johansson U. Immunocytochemical Profiling of Cultured Mouse Primary Retinal Cells. J Histochem Cytochem. 2017;65:223-239 pubmed publisher
    ..17 retinal markers, that is, recoverin, rhodopsin, arrestin, Chx10, PKC, DCX, CRALBP, GS, vimentin, TPRV4, RBPMS, Brn3a, β-tubulin III, NeuN, MAP2, GFAP, and synaptophysin...
  13. Alves M, Boia R, Campos E, Martins J, Nunes S, Madeira M, et al. Subtle thinning of retinal layers without overt vascular and inflammatory alterations in a rat model of prediabetes. Mol Vis. 2018;24:353-366 pubmed
    ..transferase (TdT) dUTP nick-end labeling (TUNEL) assay and by evaluating the immunoreactivity of the Brn3a transcription factor...
  14. Maekawa Y, Onishi A, Matsushita K, Koide N, Mandai M, Suzuma K, et al. Optimized Culture System to Induce Neurite Outgrowth From Retinal Ganglion Cells in Three-Dimensional Retinal Aggregates Differentiated From Mouse and Human Embryonic Stem Cells. Curr Eye Res. 2016;41:558-68 pubmed publisher
    ..First, we confirmed that Venus-positive cells were double-labeled with a RGC marker, Brn3a, in the 3D-retina differentiated from Fstl4::Venus mouse ESCs, indicating specific RGC-subtype differentiation...
  15. Blanchard J, Eade K, Szucs A, Lo Sardo V, Tsunemoto R, Williams D, et al. Selective conversion of fibroblasts into peripheral sensory neurons. Nat Neurosci. 2015;18:25-35 pubmed publisher
    ..We found that transiently coexpressing Brn3a with either Ngn1 or Ngn2 selectively reprogrammed human and mouse fibroblasts to acquire key properties of these ..
  16. Nadal Nicolás F, Valiente Soriano F, Salinas Navarro M, Jiménez López M, Vidal Sanz M, Agudo Barriuso M. Retino-retinal projection in juvenile and young adult rats and mice. Exp Eye Res. 2015;134:47-52 pubmed publisher
    ..Right retinas were dissected as flat-mounts and double immunodetected for Brn3a and melanopsin. Ret-ret RGCs were those with tracer accumulation in their somas...
  17. Corso Díaz X, Simpson E. Nr2e1 regulates retinal lamination and the development of Müller glia, S-cones, and glycineric amacrine cells during retinogenesis. Mol Brain. 2015;8:37 pubmed publisher
    ..Müller glia were mispositioned in the retina and misexpressed the ganglion cell-specific transcription factor Brn3a. Nr2e1 (frc/frc) retinas also displayed lamination defects including an ectopic neuropil forming an additional ..
  18. Ishii J, Sato H, Yazawa T, Shishido Hara Y, Hiramatsu C, Nakatani Y, et al. Class III/IV POU transcription factors expressed in small cell lung cancer cells are involved in proneural/neuroendocrine differentiation. Pathol Int. 2014;64:415-22 pubmed publisher
    ..Because class III POU genes (POU3F1, POU3F2, POU3F3, and POU3F4) and class IV POU genes (POU4F1, POU4F2, and POU4F3) share similar properties in neural development, we analyzed the association between class III/..
  19. Mead B, Thompson A, Scheven B, Logan A, Berry M, Leadbeater W. Comparative evaluation of methods for estimating retinal ganglion cell loss in retinal sections and wholemounts. PLoS ONE. 2014;9:e110612 pubmed publisher
    ..Adult rats received a unilateral ONC and after 21 days the numbers of Brn3a(+), βIII-tubulin(+) and Islet-1(+) RGCs were quantified in either retinal radial sections or wholemounts in ..
  20. Nagalski A, Puelles L, Dabrowski M, Wegierski T, Kuznicki J, Wisniewska M. Molecular anatomy of the thalamic complex and the underlying transcription factors. Brain Struct Funct. 2016;221:2493-510 pubmed publisher
    ..divisions of the adult thalamic complex are defined by the combinatorial expression of Tcf7l2, Lef1, Gbx2, Prox1, Pou4f1, Esrrg, and Six3 transcription factor genes...
  21. Badea T, Williams J, Smallwood P, Shi M, Motajo O, Nathans J. Combinatorial expression of Brn3 transcription factors in somatosensory neurons: genetic and morphologic analysis. J Neurosci. 2012;32:995-1007 pubmed publisher
    The three members of the Brn3 family of POU-domain transcription factors (Brn3a/Pou4f1, Brn3b/Pou4f2, and Brn3c/Pou4f3) are expressed in overlapping subsets of visual, auditory/vestibular, and somatosensory neurons...
  22. Martinez Lopez J, Moreno Bravo J, Madrigal M, Martinez S, Puelles E. Red nucleus and rubrospinal tract disorganization in the absence of Pou4f1. Front Neuroanat. 2015;9:8 pubmed publisher
    ..b>Pou4f1 is a transcription factor highly expressed in this nucleus that has been related to its specification...
  23. Rai R, Chauhan S, Singh V, Rai M, Rai G. Heat shock protein 27 and its regulatory molecules express differentially in SLE patients with distinct autoantibody profiles. Immunol Lett. 2015;164:25-32 pubmed publisher
    ..anti-apoptotic properties hence it was of interest to study the expression of HSP27 and its regulatory molecule Brn3a and hsa-miR-939 in SLE patients with distinct autoantibodies specificities...
  24. Valiente Soriano F, Nadal Nicolás F, Salinas Navarro M, Jiménez López M, Bernal Garro J, Villegas Pérez M, et al. BDNF Rescues RGCs But Not Intrinsically Photosensitive RGCs in Ocular Hypertensive Albino Rat Retinas. Invest Ophthalmol Vis Sci. 2015;56:1924-36 pubmed publisher
    To study the responses of the general population of retinal ganglion cells (Brn3a(+)RGCs) versus the intrinsically photosensitive RGCs (melanopsin-expressing RGCs [m(+)RGCs]) to ocular hypertension (OHT), the effects of brain-derived ..
  25. Del Olmo Aguado S, Núñez Álvarez C, Osborne N. Red light of the visual spectrum attenuates cell death in culture and retinal ganglion cell death in situ. Acta Ophthalmol. 2016;94:e481-91 pubmed publisher
    ..ganglion cell numbers and an elevation (GFAP, vimentin, HO-1 and mTORC1) or reduction (Thy-1 and Brn3a) of mRNAs in the rat retina. These negative effects to the rat retina caused by ischaemia are reduced by red light...
  26. Muller Q, Beaudet M, De Serres Bérard T, Bellenfant S, Flacher V, Berthod F. Development of an innervated tissue-engineered skin with human sensory neurons and Schwann cells differentiated from iPS cells. Acta Biomater. 2018;82:93-101 pubmed publisher
    ..Nearly 100% of the iPSC differentiated into sensory neurons were shown to express the neuronal markers BRN3A and β3-tubulin after 19 days of maturation...
  27. Schitine C, Méndez Flores O, Santos L, Ornelas I, Calaza K, Pérez Toledo K, et al. Functional plasticity of GAT-3 in avian Müller cells is regulated by neurons via a glutamatergic input. Neurochem Int. 2015;82:42-51 pubmed publisher
    ..Our data suggest that in purified cultures and upon extensive neuronal lesion in vivo, shown as a Brn3a reduced neuronal cells and an GFAP increased gliosis, Müller glia may change its capacity to take up GABA due ..
  28. Ghinia M, Novelli E, Sajgo S, Badea T, Strettoi E. Brn3a and Brn3b knockout mice display unvaried retinal fine structure despite major morphological and numerical alterations of ganglion cells. J Comp Neurol. 2019;527:187-211 pubmed publisher
    ..Using Brn3a and Brn3b mutant mice in which the alkaline phosphatase gene was knocked-in (Badea et al...
  29. Anders F, Teister J, Funke S, Pfeiffer N, Grus F, Solon T, et al. Proteomic profiling reveals crucial retinal protein alterations in the early phase of an experimental glaucoma model. Graefes Arch Clin Exp Ophthalmol. 2017;255:1395-1407 pubmed publisher
    ..Morphological changes were surveyed on a cellular level with a staining of Brn3a-positive cells...
  30. Esquiva G, Avivi A, Hannibal J. Non-image Forming Light Detection by Melanopsin, Rhodopsin, and Long-Middlewave (L/W) Cone Opsin in the Subterranean Blind Mole Rat, Spalax Ehrenbergi: Immunohistochemical Characterization, Distribution, and Connectivity. Front Neuroanat. 2016;10:61 pubmed publisher
    ..The remaining RGCs were shown to co-store Brn3a and calretinin...
  31. Hirano T, Murata T, Hayashi T. Physiological significance of recombination-activating gene 1 in neuronal death, especially optic neuropathy. FEBS J. 2015;282:129-41 pubmed publisher
    ..study, experiments with genetically engineered mice revealed the involvement of Rag1 expression in apoptosis of Brn3a-positive retinal ganglion cells, and also demonstrated the specific effect of p50 deficiency on the activation of ..
  32. Chintala S, Putris N, Geno M. Activation of TLR3 promotes the degeneration of retinal ganglion cells by upregulating the protein levels of JNK3. Invest Ophthalmol Vis Sci. 2015;56:505-14 pubmed publisher
    ..degeneration of RGCs was assessed by using antibodies against brain-specific homeobox/POU domain protein 3a (Brn3a). A TLR3-specific inhibitor was injected into the vitreous humor with or without Poly(I:C)...
  33. Nadal Nicolás F, Madeira M, Salinas Navarro M, Jiménez López M, Galindo Romero C, Ortín Martínez A, et al. Transient Downregulation of Melanopsin Expression After Retrograde Tracing or Optic Nerve Injury in Adult Rats. Invest Ophthalmol Vis Sci. 2015;56:4309-23 pubmed publisher
    ..b>Brn3a and melanopsin were double stained in whole mounts to quantify and assess the distribution of orthotopic and ..
  34. Sajgo S, Ali S, Popescu O, Badea T. Dynamic expression of transcription factor Brn3b during mouse cranial nerve development. J Comp Neurol. 2016;524:1033-61 pubmed publisher
    ..b>Brn3a, Brn3b, and Brn3c, in combination with each other and/or transcription factors of other families, can define ..
  35. Nouri N, Patel M, Joksimovic M, Poulin J, Anderegg A, Taketo M, et al. Excessive Wnt/beta-catenin signaling promotes midbrain floor plate neurogenesis, but results in vacillating dopamine progenitors. Mol Cell Neurosci. 2015;68:131-42 pubmed publisher
    ..We also show by lineage tracing analysis that normally, Neurog1+ progenitors predominantly give rise to Pou4f1+ neurons, but not Th+ neurons...
  36. Vidal Sanz M, Valiente Soriano F, Ortín Martínez A, Nadal Nicolás F, Jiménez López M, Salinas Navarro M, et al. Retinal neurodegeneration in experimental glaucoma. Prog Brain Res. 2015;220:1-35 pubmed publisher
    ..retrograde axonal transport, melanopsin immunodetection to identify intrinsically photosensitive RGCs (m(+)RGC), Brn3a immunodetection to identify most RGCs but not m(+)RGCs, RECA1 immunodetection to examine the inner retinal vessels,..
  37. Nadal Nicolás F, Jiménez López M, Salinas Navarro M, Sobrado Calvo P, Vidal Sanz M, Agudo Barriuso M. Microglial dynamics after axotomy-induced retinal ganglion cell death. J Neuroinflammation. 2017;14:218 pubmed publisher
    ..from 2 days to 2 months after the lesions (n = 4-12 group/lesion and time point) and then were subjected to Brn3a and Iba1 double immunodetection...
  38. Chuang J, Yarmishyn A, Hwang D, Hsu C, Wang M, Yang Y, et al. Expression profiling of cell-intrinsic regulators in the process of differentiation of human iPSCs into retinal lineages. Stem Cell Res Ther. 2018;9:140 pubmed publisher
    ..EBF1 was shown to act upstream of ISL1 and BRN3A, the well-characterized regulators of RGC lineage specification...
  39. Budhram Mahadeo V, Fujita R, Bitsi S, Sicard P, Heads R. Co-expression of POU4F2/Brn-3b with p53 may be important for controlling expression of pro-apoptotic genes in cardiomyocytes following ischaemic/hypoxic insults. Cell Death Dis. 2014;5:e1503 pubmed publisher
    ..In contrast, the related POU4F1/Brn-3a (Brn-3a) blocks p53-mediated apoptosis but co-operates with p53 to enhance cell cycle arrest...
  40. González Fleitas M, Bordone M, Rosenstein R, Dorfman D. Effect of retinal ischemia on the non-image forming visual system. Chronobiol Int. 2015;32:152-63 pubmed publisher
    ..At this time point, a significant decrease in the number of Brn3a(+) RGCs and in the anterograde transport from the retina to the superior colliculus and lateral geniculate nucleus ..
  41. Tzekov R, Dawson C, Orlando M, Mouzon B, Reed J, Evans J, et al. Sub-Chronic Neuropathological and Biochemical Changes in Mouse Visual System after Repetitive Mild Traumatic Brain Injury. PLoS ONE. 2016;11:e0153608 pubmed publisher
    ..These changes were accompanied by a ~25% decrease in the total number of Brn3a-positive RGCs...
  42. Badea T, Nathans J. Morphologies of mouse retinal ganglion cells expressing transcription factors Brn3a, Brn3b, and Brn3c: analysis of wild type and mutant cells using genetically-directed sparse labeling. Vision Res. 2011;51:269-79 pubmed publisher
    ..The roles of the closely related family members, Brn3a/Pou4f1 and Brn3c/Pou4f3 in RGC development are less clear...
  43. Yukita M, Machida S, Nishiguchi K, Tsuda S, Yokoyama Y, Yasuda M, et al. Molecular, anatomical and functional changes in the retinal ganglion cells after optic nerve crush in mice. Doc Ophthalmol. 2015;130:149-56 pubmed publisher
    ..The expression of the RGC markers Brn3a and Brn3b was measured on days 1, 2, 3, 5 and 7 after ONC with quantitative real-time PCR...
  44. Ankam S, Lim C, Yim E. Actomyosin contractility plays a role in MAP2 expression during nanotopography-directed neuronal differentiation of human embryonic stem cells. Biomaterials. 2015;47:20-8 pubmed publisher
    ..On the other hand, our qPCR array results showed that PAX5, BRN3A and NEUROD1 were highly expressed in hESCs grown on nano-grating substrates as compared to unpatterned substrates, ..
  45. Qu C, Bian D, Li X, Xiao J, Wu C, Li Y, et al. Transient Expression of Fez Family Zinc Finger 2 Protein Regulates the Brn3b Gene in Developing Retinal Ganglion Cells. J Biol Chem. 2016;291:7661-8 pubmed publisher
    ..Fezf2knockdown by retinalin uteroelectroporation diminished BRN3B but not the coexpressed ISLET1 and BRN3A, indicating that the BRN3B decrease was the cause, not the result, of the overall reduction of BRN3B+ RGCs in ..
  46. Serrano Saiz E, Leyva Díaz E, De La Cruz E, Hobert O. BRN3-type POU Homeobox Genes Maintain the Identity of Mature Postmitotic Neurons in Nematodes and Mice. Curr Biol. 2018;28:2813-2823.e2 pubmed publisher
    ..Similarly, the mouse unc-86 ortholog Brn3a/POU4F1 has been shown to control the initiation of the terminal differentiation program of distinct neuron types across ..
  47. Yan R, He L, Zhan W, Wang S. Induction of ectopic retina-like tissue by transgenic expression of neurogenin. PLoS ONE. 2015;10:e0116171 pubmed publisher
    ..label other types of retinal neurons, including AP2α and Pax6 for amacrine cells, Otx2 for bipolar cells, and Brn3A for ganglion cells...
  48. Aranda M, Dorfman D, Sande P, Rosenstein R. Experimental optic neuritis induced by the microinjection of lipopolysaccharide into the optic nerve. Exp Neurol. 2015;266:30-41 pubmed publisher
    ..protein immunoreactivity and luxol fast blue staining), viii) optic nerve ultrastructure, and ix) RGC number (by Brn3a immunoreactivity)...
  49. Valiente Soriano F, Salinas Navarro M, Jiménez López M, Alarcón Martínez L, Ortín Martínez A, Bernal Garro J, et al. Effects of ocular hypertension in the visual system of pigmented mice. PLoS ONE. 2015;10:e0121134 pubmed publisher
    ..To determine RGC survival, these same retinas were immunoreacted against Brn3a (general RGC population) and melanopsin (intrinsically photosensitive RGCs, m+RGCs)...
  50. Chintalapudi S, Djenderedjian L, Stiemke A, Steinle J, Jablonski M, Morales Tirado V. Isolation and Molecular Profiling of Primary Mouse Retinal Ganglion Cells: Comparison of Phenotypes from Healthy and Glaucomatous Retinas. Front Aging Neurosci. 2016;8:93 pubmed publisher
    ..of homogeneous sorted cells for the intracellular RGC-markers SNCG, brain-specific homeobox/POU domain protein 3A (BRN3A), TUJ1, and RNA-binding protein with multiple splicing (RBPMS); and (3) by applying the methodology on RGC from a ..
  51. Hsu Y, Wang S, Wang S, Morton G, Zariwala H, de la Iglesia H, et al. Role of the dorsal medial habenula in the regulation of voluntary activity, motor function, hedonic state, and primary reinforcement. J Neurosci. 2014;34:11366-84 pubmed publisher
    ..medial habenula (dMHb) are developmentally eliminated, via tissue-specific deletion of the transcription factor Pou4f1 (Brn3a)...
  52. Ortín Martínez A, Salinas Navarro M, Nadal Nicolás F, Jiménez López M, Valiente Soriano F, García Ayuso D, et al. Laser-induced ocular hypertension in adult rats does not affect non-RGC neurons in the ganglion cell layer but results in protracted severe loss of cone-photoreceptors. Exp Eye Res. 2015;132:17-33 pubmed publisher
    ..To investigate the ganglion cell layer (GCL) wholemounts were examined at 1, 3 or 6 months using Brn3a-immunodetection to identify retinal ganglion cells (RGCs) and DAPI-staining to detect all nuclei in this layer...
  53. Rovere G, Nadal Nicolás F, Sobrado Calvo P, García Bernal D, Villegas Pérez M, Vidal Sanz M, et al. Topical Treatment With Bromfenac Reduces Retinal Gliosis and Inflammation After Optic Nerve Crush. Invest Ophthalmol Vis Sci. 2016;57:6098-6106 pubmed publisher
    ..Quantification of Brn3a (brain-specific homeobox/POU domain protein 3A) +RGCs (retinal ganglion cells) in cross sections showed that ..
  54. Hsu Y, Gile J, Perez J, Morton G, Ben Hamo M, Turner E, et al. The Dorsal Medial Habenula Minimally Impacts Circadian Regulation of Locomotor Activity and Sleep. J Biol Rhythms. 2017;32:444-455 pubmed publisher
    ..demonstrated that WRA is markedly reduced in mice with a region-specific deletion of the transcription factor Pou4f1 (Brn3a), which leads to an ablation of the dorsal medial habenula (dMHb)...
  55. Muzyka V, Brooks M, Badea T. Postnatal developmental dynamics of cell type specification genes in Brn3a/Pou4f1 Retinal Ganglion Cells. Neural Dev. 2018;13:15 pubmed publisher
    ..The developmental mechanisms by which RGCs are specified are still largely unknown. Brn3a is a member of the Brn3/Pou4f transcription factor family, which contains key regulators of RGC postmitotic ..
  56. Lee V, Hosking B, Holeniewska J, Kubala E, Lundh von Leithner P, Gardner P, et al. BTBR ob/ob mouse model of type 2 diabetes exhibits early loss of retinal function and retinal inflammation followed by late vascular changes. Diabetologia. 2018;61:2422-2432 pubmed publisher
    ..Animals were weighed and blood glucose was measured. TUNEL and brain-specific homeobox/POU domain protein 3A (BRN3A) markers were used to examine retinal ganglion cells...
  57. Sajgo S, Ghinia M, Brooks M, Kretschmer F, Chuang K, Hiriyanna S, et al. Molecular codes for cell type specification in Brn3 retinal ganglion cells. Proc Natl Acad Sci U S A. 2017;114:E3974-E3983 pubmed publisher
    ..We use deep sequencing to define (i) transcriptomes of Brn3a- and/or Brn3b-positive RGCs, (ii) Brn3a- and/or Brn3b-dependent RGC transcripts, and (iii) ..
  58. Sánchez Migallón M, Valiente Soriano F, Nadal Nicolás F, Di Pierdomenico J, Vidal Sanz M, Agudo Barriuso M. Survival of melanopsin expressing retinal ganglion cells long term after optic nerve trauma in mice. Exp Eye Res. 2018;174:93-97 pubmed publisher
    ..of the general population of retinal ganglion cells in charge of the image-forming visual functions that express Brn3a (Brn3a+RGCs) with that of the sub-population of non-image forming RGCs that express melanopsin (m+<..
  59. Jiang H, Du J, Song J, Li Y, Wu M, Zhou J, et al. Loss-of-function mutation of serine racemase attenuates retinal ganglion cell loss in diabetic mice. Exp Eye Res. 2018;175:90-97 pubmed publisher
    ..identified with retrograde labeling in vivo or with immunolabeling against a RGC-specific transcription factor, Brn3a, in retinal flat mounts...
  60. Grigsby K, Kelty T, Booth F. Medial habenula maturational deficits associate with low motivation for voluntary physical activity. Brain Res. 2018;1698:187-194 pubmed publisher
    ..We report a significantly higher expression of genes associated with MHb development (Brn3a, Nurr1, Tac1, and Kcnip) in HVR versus LVR rats...
  61. Maskell L, Qamar K, Babakr A, Hawkins T, Heads R, Budhram Mahadeo V. Essential but partially redundant roles for POU4F1/Brn-3a and POU4F2/Brn-3b transcription factors in the developing heart. Cell Death Dis. 2017;8:e2861 pubmed publisher
    ..Here, we report that transcription factors (TFs) Brn-3a (POU4F1) and Brn-3b (POU4F2) are important for normal cardiac development...
  62. Sánchez Migallón M, Valiente Soriano F, Salinas Navarro M, Nadal Nicolás F, Jiménez López M, Vidal Sanz M, et al. Nerve fibre layer degeneration and retinal ganglion cell loss long term after optic nerve crush or transection in adult mice. Exp Eye Res. 2018;170:40-50 pubmed publisher
    ..RGCs were immunoidentified with anti-Brn3a, and their axons with anti-highly phosphorylated axonal neurofilament subunit H (pNFH)...
  63. Ma W, Wei X, Gu H, Li H, Guan K, Liu D, et al. Sensory neuron differentiation potential of in utero mesenchymal stem cell transplantation in rat fetuses with spina bifida aperta. Birth Defects Res A Clin Mol Teratol. 2015;103:772-9 pubmed publisher
    ..The fetuses were harvested on E20. Immunofluorescence was used to detect the expression of Brn3a protein in the transplanted MSCs and dorsal root ganglion (DRG) neurons in the defective spinal cords...
  64. Chen J, Riazifar H, Guan M, Huang T. Modeling autosomal dominant optic atrophy using induced pluripotent stem cells and identifying potential therapeutic targets. Stem Cell Res Ther. 2016;7:2 pubmed publisher
    ..differentiated into putative RGCs, which were subsequently characterized by using RGC-specific expression markers BRN3a and ISLET-1...
  65. Sapienza A, Raveu A, Reboussin E, Roubeix C, Boucher C, Dégardin J, et al. Bilateral neuroinflammatory processes in visual pathways induced by unilateral ocular hypertension in the rat. J Neuroinflammation. 2016;13:44 pubmed publisher
    ..RGCs immunolabeled (Brn3a(+)) in ipsilateral whole flat-mounted retina demonstrated peripheral RGC loss associated with tissue macrophage/..
  66. Schäck L, Budde S, Lenarz T, Krettek C, Gross G, Windhagen H, et al. Induction of neuronal-like phenotype in human mesenchymal stem cells by overexpression of Neurogenin1 and treatment with neurotrophins. Tissue Cell. 2016;48:524-32 pubmed publisher
    ..PCR, the up-regulation of transcription factors expressed by developing primary auditory neurons, such as BRN3a (POU4F1) and GATA3, was quantified after induction of Neurog-1 expression...
  67. Riddiford N, Schlosser G. Dissecting the pre-placodal transcriptome to reveal presumptive direct targets of Six1 and Eya1 in cranial placodes. elife. 2016;5: pubmed publisher
    ..g. Sox2, Hes8) and neuronal/sensory differentiation (e.g. Ngn1, Atoh1, Pou4f1, Gfi1) require Six1 and Eya1 for their placodal expression...
  68. Alldredge A, Fuhrmann S. Loss of Axin2 Causes Ocular Defects During Mouse Eye Development. Invest Ophthalmol Vis Sci. 2016;57:5253-5262 pubmed publisher
    ..Activation of the Axin2lacZ reporter overlapped with labeling of POU4F1, PAX6, and Calbindin...
  69. Wang F, Dang Y, Wang J, Zhou T, Zhu Y. Gypenosides attenuate lipopolysaccharide-induced optic neuritis in rats. Acta Histochem. 2018;120:340-346 pubmed publisher
    ..Apoptosis of retinal ganglion cells (RGCs) was evaluated using Brn3a-TUNEL double staining...
  70. Trost A, Motloch K, Bruckner D, Schroedl F, Bogner B, Kaser Eichberger A, et al. Time-dependent retinal ganglion cell loss, microglial activation and blood-retina-barrier tightness in an acute model of ocular hypertension. Exp Eye Res. 2015;136:59-71 pubmed publisher
    ..microvascular pericyte (PC) coverage was analyzed using immunohistochemistry with corresponding specific markers (Brn3a, Iba1, NG2)...
  71. Dal Monte M, Cammalleri M, Locri F, Amato R, Marsili S, Rusciano D, et al. Fatty Acids Dietary Supplements Exert Anti-Inflammatory Action and Limit Ganglion Cell Degeneration in the Retina of the EAE Mouse Model of Multiple Sclerosis. Nutrients. 2018;10: pubmed publisher
    ..Immunolabeling of retinal whole mounts with a Brn3a antibody demonstrated drastic RGC loss...
  72. Chintala S. Tissue and urokinase plasminogen activators instigate the degeneration of retinal ganglion cells in a mouse model of glaucoma. Exp Eye Res. 2016;143:17-27 pubmed publisher
    ..RGCs' degeneration was assessed by immunostaining with antibodies against Brn3a. Injection of microbeads into the anterior chamber led to a progressive elevation in IOP, increased the ..
  73. García Ayuso D, Di Pierdomenico J, Esquiva G, Nadal Nicolás F, Pinilla I, Cuenca N, et al. Inherited Photoreceptor Degeneration Causes the Death of Melanopsin-Positive Retinal Ganglion Cells and Increases Their Coexpression of Brn3a. Invest Ophthalmol Vis Sci. 2015;56:4592-604 pubmed publisher
    ..and Sprague Dawley (SD) rats (control) were dissected as whole-mounts and immunodetected for melanopsin and/or Brn3a. The dendritic arborization of m+RGCs and the numbers of Brn3a+RGCs and m+RGCs were quantified and their retinal ..
  74. Mead B, Hill L, Blanch R, Ward K, Logan A, Berry M, et al. Mesenchymal stromal cell-mediated neuroprotection and functional preservation of retinal ganglion cells in a rodent model of glaucoma. Cytotherapy. 2016;18:487-96 pubmed publisher
    ..Thirty-five days after raised intraocular pressure (IOP) and intravitreal stem cell transplantation, Brn3a(+) RGC numbers, retinal nerve fibre layer thickness (RNFL) and RGC function were evaluated by immunohistochemistry,..
  75. Hsu Y, Morton G, Guy E, Wang S, Turner E. Dorsal Medial Habenula Regulation of Mood-Related Behaviors and Primary Reinforcement by Tachykinin-Expressing Habenula Neurons. Eneuro. 2016;3: pubmed publisher
    ..Tissue-specific deletion of the transcription factor Pou4f1 in the dorsal MHb (dMHb) results in a developmental lesion of this subnucleus...
  76. Sato C, Iwai Takekoshi L, Ichikawa Y, Kawasaki H. Cell type-specific expression of FoxP2 in the ferret and mouse retina. Neurosci Res. 2017;117:1-13 pubmed publisher
    ..Large FoxP2-positive neurons expressed Brn3a and were retrogradely labeled with cholera toxin subunit B injected into the optic nerve, indicating that they are ..
  77. Kuse Y, Tsuruma K, Mizoguchi T, Shimazawa M, Hara H. Progranulin deficiency causes the retinal ganglion cell loss during development. Sci Rep. 2017;7:1679 pubmed publisher
    ..Our findings showed that the number of Brn3a-positive cells was reduced and the expression of glial fibrillary acidic protein (GFAP) was increased in ..
  78. Huang R, Lan Q, Chen L, Zhong H, Cui L, Jiang L, et al. CD200Fc Attenuates Retinal Glial Responses and RGCs Apoptosis After Optic Nerve Crush by Modulating CD200/CD200R1 Interaction. J Mol Neurosci. 2018;64:200-210 pubmed publisher
    ..The density of RGCs was counted by immunostaining of retina flat mounts for Brn3a. TUNEL assay, immunoblotting analysis of ionized calcium-binding adapter molecule 1(iba1) (microglia marker) and ..
  79. Chintala S, Cheng M, Zhang X. Decreased Expression of DREAM Promotes the Degeneration of Retinal Neurons. PLoS ONE. 2015;10:e0127776 pubmed publisher
    ..of RGCs in cross sections and in whole mount retinas was determined by using antibodies against Tuj1 and Brn3a respectively...
  80. Gramlich O, Teister J, Neumann M, Tao X, Beck S, von Pein H, et al. Immune response after intermittent minimally invasive intraocular pressure elevations in an experimental animal model of glaucoma. J Neuroinflammation. 2016;13:82 pubmed publisher
    ..Retinal cross-sections were immunostained against Brn3a, Iba1, and IgG autoantibody depositions...
  81. Davis B, Tian K, Pahlitzsch M, Brenton J, Ravindran N, Butt G, et al. Topical Coenzyme Q10 demonstrates mitochondrial-mediated neuroprotection in a rodent model of ocular hypertension. Mitochondrion. 2017;36:114-123 pubmed publisher
    ..retinal cell health was assessed in vivo using DARC (Detection of Apoptotic Retinal Cells) and post-mortem with Brn3a histological assessment on whole retinal mounts...
  82. Zhang C, Xu Y, Tan H, Li S, Wang N, Zhang Y, et al. Neuroprotective effect of He-Ying-Qing-Re formula on retinal ganglion cell in diabetic retinopathy. J Ethnopharmacol. 2018;214:179-189 pubmed publisher
    ..HF attenuates the loss of RGCs, thinning of inner retinal layers in diabetic mice. Furthermore, expressions of Brn3a and PSD-95 were restored while CHOP level was downregulated upon HF treatment...
  83. Zheng D, Yang X, Sheng D, Yu D, Liang G, Guo L, et al. Pou4f2-GFP knock-in mouse line: A model for studying retinal ganglion cell development. Genesis. 2016;54:534-541 pubmed publisher
    ..Compared with those in wild-type mice, the expression patterns of POU4F2 and POU4F1 and the co-expression patterns of ISL1 and POU4F2 were unaffected in Pou4f2-GFP/GFP homozygote mice...
  84. Xu Y, Yang B, Hu Y, Lu L, Lu X, Wang J, et al. Wogonin prevents TLR4-NF-κB-medicated neuro-inflammation and improves retinal ganglion cells survival in retina after optic nerve crush. Oncotarget. 2016;7:72503-72517 pubmed publisher
    ..In this study, wogonin treatment reduced RGCs loss and inhibited RGCs apoptosis demonstrated by the increased Brn3a labeling RGCs at day 14 and the decreased cleaved caspase-3 expression at day 7 after ONC, respectively...
  85. Groman Lupa S, Adewumi J, Park K, Brzezinski J. The Transcription Factor Prdm16 Marks a Single Retinal Ganglion Cell Subtype in the Mouse Retina. Invest Ophthalmol Vis Sci. 2017;58:5421-5433 pubmed publisher
    ..Prdm16 was expressed in the retina starting late in embryogenesis. Prdm16+ cells coexpressed the RGC marker Brn3a. These cells were arranged in an evenly spaced pattern and accounted for 2% of all ganglion cells...
  86. Brito R, Pereira Figueiredo D, Socodato R, Paes de Carvalho R, Calaza K. Caffeine exposure alters adenosine system and neurochemical markers during retinal development. J Neurochem. 2016;138:557-70 pubmed publisher
    ..showed a decrease in retinal content of tyrosine hydroxylase, calbindin and choline acetyltransferase, but not Brn3a, after 48 h of caffeine injection...
  87. Blanco R, Martínez Navarrete G, Valiente Soriano F, Avilés Trigueros M, Pérez Rico C, Serrano Puebla A, et al. The S1P1 receptor-selective agonist CYM-5442 protects retinal ganglion cells in endothelin-1 induced retinal ganglion cell loss. Exp Eye Res. 2017;164:37-45 pubmed publisher
    ..nerve fiber layer (RNFL) thickness were assessed in vivo using optical coherence tomography and ex vivo using Brn3a immunohistochemistry in flat-mounted retinas...
  88. Qing X, Svaren J, Keith I. mRNA expression of novel CGRP1 receptors and their activity-modifying proteins in hypoxic rat lung. Am J Physiol Lung Cell Mol Physiol. 2001;280:L547-54 pubmed
    ..Our results show upregulation of RDC-1, RAMP1, and RAMP3 mRNAs in hypoxic rat lung and no change in CRLR and RAMP2 mRNAs. These findings support a functional role for CGRP and ADM receptors in regulating the adult pulmonary circulation...
  89. Webb R, Gallegos Cárdenas A, Miller C, Solomotis N, Liu H, West F, et al. Pig Induced Pluripotent Stem Cell-Derived Neural Rosettes Parallel Human Differentiation Into Sensory Neural Subtypes. Cell Reprogram. 2017;19:88-94 pubmed publisher
    ..Treatment with either bone morphogenetic protein 4 or fetal bovine serum led to differentiation into BRN3A-positive sensory cells and increased expression of sensory neuron TRK receptor gene family: TRKA, TRKB, and TRKC...
  90. García Ayuso D, Galindo Romero C, Di Pierdomenico J, Vidal Sanz M, Agudo Barriuso M, Villegas Pérez M. Light-induced retinal degeneration causes a transient downregulation of melanopsin in the rat retina. Exp Eye Res. 2017;161:10-16 pubmed publisher
    ..Whole-mounted retinas were immunodetected with antibodies against melanopsin, Brn3a, and rhodopsin to study the populations of m+RGC, Brn3a+RGC and rods (which are the most ..
  91. Budhram Mahadeo V, Parker M, Latchman D. POU transcription factors Brn-3a and Brn-3b interact with the estrogen receptor and differentially regulate transcriptional activity via an estrogen response element. Mol Cell Biol. 1998;18:1029-41 pubmed
    ..These observations and their implications for transcriptional regulation by the ER are discussed. ..
  92. Ndisang D, Faulkes D, Gascoyne D, Lee S, Ripley B, Sindos M, et al. Differential regulation of different human papilloma virus variants by the POU family transcription factor Brn-3a. Oncogene. 2006;25:51-60 pubmed
    ..These results help explain why the specific interplay between viral and cellular factors necessary for the progression to cervical carcinoma only occurs in a minority of those infected with HPV-16. ..