POM121

Summary

Gene Symbol: POM121
Description: POM121 transmembrane nucleoporin
Alias: P145, POM121A, nuclear envelope pore membrane protein POM 121, POM121 membrane glycoprotein, nuclear envelope pore membrane protein POM 121A, nuclear pore membrane protein 121 kDa, nucleoporin Nup121
Species: human
Products:     POM121

Top Publications

  1. Hallberg E, Wozniak R, Blobel G. An integral membrane protein of the pore membrane domain of the nuclear envelope contains a nucleoporin-like region. J Cell Biol. 1993;122:513-21 pubmed
    ..We obtained peptide sequence from purified p145 and cloned and sequenced several cDNA clones and one genomic clone...
  2. Funakoshi T, Maeshima K, Yahata K, Sugano S, Imamoto F, Imamoto N. Two distinct human POM121 genes: requirement for the formation of nuclear pore complexes. FEBS Lett. 2007;581:4910-6 pubmed
    b>Pom121 is one of the integral membrane components of the nuclear pore complex (NPC) in vertebrate cells. Unlike rodent cells carrying a single POM121 gene, human cells possess multiple POM121 gene loci on chromosome 7q11...
  3. Mitchell J, Mansfeld J, Capitanio J, Kutay U, Wozniak R. Pom121 links two essential subcomplexes of the nuclear pore complex core to the membrane. J Cell Biol. 2010;191:505-21 pubmed publisher
    ..is likely closely linked to events at the membrane as we show that Nup155 interacts with pore membrane proteins Pom121 and NDC1...
  4. Eriksson C, Rustum C, Hallberg E. Dynamic properties of nuclear pore complex proteins in gp210 deficient cells. FEBS Lett. 2004;572:261-5 pubmed
    ..b>POM121 (the other integral NPC protein) and NUP107 (of the NUP107/160 complex) were correctly distributed at the nuclear ..
  5. Kubitscheck U, GRUNWALD D, Hoekstra A, Rohleder D, Kues T, Siebrasse J, et al. Nuclear transport of single molecules: dwell times at the nuclear pore complex. J Cell Biol. 2005;168:233-43 pubmed
    ..The nucleoporin Nup358 could be localized at a distance of 70 nm from POM121-GFP along the NPC axis...
  6. Yavuz S, Santarella Mellwig R, Koch B, Jaedicke A, Mattaj I, Antonin W. NLS-mediated NPC functions of the nucleoporin Pom121. FEBS Lett. 2010;584:3292-8 pubmed publisher
    ..We investigated the interplay between import receptors and the transmembrane nucleoporin Pom121. We found that Pom121 interacts with importin alpha/beta and a group of nucleoporins in an NLS-dependent manner...
  7. Daigle N, Beaudouin J, Hartnell L, Imreh G, Hallberg E, Lippincott Schwartz J, et al. Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells. J Cell Biol. 2001;154:71-84 pubmed
    ..pore complex (NPC) and its relationship to the nuclear envelope (NE) was characterized in living cells using POM121-green fluorescent protein (GFP) and GFP-Nup153, and GFP-lamin B1...
  8. Rasala B, Ramos C, Harel A, Forbes D. Capture of AT-rich chromatin by ELYS recruits POM121 and NDC1 to initiate nuclear pore assembly. Mol Biol Cell. 2008;19:3982-96 pubmed publisher
    ..Next, we find that recruitment of vesicles containing the key integral membrane pore proteins POM121 and NDC1 to the forming nucleus is dependent on chromatin-bound ELYS/Nup107-160 complex, whereas recruitment of ..
  9. Stavru F, Nautrup Pedersen G, Cordes V, Gorlich D. Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells. J Cell Biol. 2006;173:477-83 pubmed
    So far, POM121 and gp210 are the only known anchoring sites of vertebrate nuclear pore complexes (NPCs) within the lipid bilayer of the nuclear envelope (NE) and, thus, are excellent candidates for initiating the NPC assembly process...

More Information

Publications33

  1. Ge W, Yue Y, Xiong S. POM121 inhibits the macrophage inflammatory response by impacting NF-κB P65 nuclear accumulation. Exp Cell Res. 2019;: pubmed publisher
    The nuclear pore membrane protein 121 (POM121) was originally thought to be a constitutive protein of the nuclear pore complex (NPC)...
  2. Zhou R, Shi C, Tao W, Li J, Wu J, Han Y, et al. Analysis of mucosal melanoma whole-genome landscapes reveals clinically relevant genomic aberrations. Clin Cancer Res. 2019;: pubmed publisher
    ..1%) in MMs, our study also found that (i) mutations and amplifications in the transmembrane nucleoporin gene POM121 (30...
  3. Denk D, Bradtke J, König M, Strehl S. PAX5 fusion genes in t(7;9)(q11.2;p13) leukemia: a case report and review of the literature. Mol Cytogenet. 2014;7:13 pubmed publisher
    ..22), ELN (7q11.23), or POM121 (7q11.23), which constitute the only currently known cluster of PAX5 partner genes...
  4. Lim K, Wong R. Targeting Nucleoporin POM121-Importin ? Axis in Prostate Cancer. Cell Chem Biol. 2018;25:1056-1058 pubmed publisher
    ..2018) demonstrates that nuclear pores promote aggressive prostate cancer by snowballing POM121-importin ?-driven nuclear import...
  5. Winterflood C, Ewers H. Single-molecule localization microscopy using mCherry. Chemphyschem. 2014;15:3447-51 pubmed publisher
    ..We were able to image the C-terminus of the nuclear pore protein POM121, which is on the inside of the pore and not readily accessible for external labeling...
  6. Guo J, Liu X, Wu C, Hu J, Peng K, Wu L, et al. The transmembrane nucleoporin Pom121 ensures efficient HIV-1 pre-integration complex nuclear import. Virology. 2018;521:169-174 pubmed publisher
    ..contrast, we found that HIV-1 replication was significantly decreased in 293T and TZM-b1 cells with siRNA-mediated Pom121 knockdown. Quantitative PCR indicated that viral replication was impaired at the step of cDNA nuclear import...
  7. Rodriguez Bravo V, Pippa R, Song W, Carceles Cordon M, Dominguez Andres A, Fujiwara N, et al. Nuclear Pores Promote Lethal Prostate Cancer by Increasing POM121-Driven E2F1, MYC, and AR Nuclear Import. Cell. 2018;174:1200-1215.e20 pubmed publisher
    ..Focused loss-of-function genetic screen of top-upregulated Nups in aggressive PC models identified POM121 as a key contributor to PC aggressiveness...
  8. Kim Y, Ahn K, Gim J, Oh M, Han K, Kim H. Gene structure variation in segmental duplication block C of human chromosome 7q 11.23 during primate evolution. Gene. 2015;573:285-95 pubmed publisher
    ..We analyzed the structure of POM121, NSUN5, FKBP6, and TRIM50 genes in the LCRs of block C...
  9. Iwamoto M, Osakada H, Mori C, Fukuda Y, Nagao K, Obuse C, et al. Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena. J Cell Sci. 2017;130:1822-1834 pubmed publisher
    ..Furthermore, the transmembrane components Pom121 and Pom82 localize exclusively to MAC and MIC NPCs, respectively...
  10. Bodoor K, Shaikh S, Enarson P, Chowdhury S, Salina D, Raharjo W, et al. Function and assembly of nuclear pore complex proteins. Biochem Cell Biol. 1999;77:321-9 pubmed
    ..been possible to define a time course of postmitotic assembly for a group of NPC components (CAN/Nup214, Nup153, POM121, p62 and Tpr) relative to the integral inner nuclear membrane protein LAP2 and the NPC membrane glycoprotein gp210...
  11. Dange T, GRUNWALD D, Grunwald A, Peters R, Kubitscheck U. Autonomy and robustness of translocation through the nuclear pore complex: a single-molecule study. J Cell Biol. 2008;183:77-86 pubmed publisher
    ..were fluorescently labeled by AlexaFluor 488, and microinjected into the cytoplasm of living HeLa cells expressing POM121-GFP as a nuclear pore marker...
  12. Kind B, Koehler K, Lorenz M, Huebner A. The nuclear pore complex protein ALADIN is anchored via NDC1 but not via POM121 and GP210 in the nuclear envelope. Biochem Biophys Res Commun. 2009;390:205-10 pubmed publisher
    ..Using a siRNA-based approach we downregulated the three known membrane integrated nucleoporins NDC1, GP210, and POM121 in stably expressing GFP-ALADIN HeLa cells...
  13. Corona A, Di Leva F, Rigogliuso G, Pescatori L, Madia V, Subra F, et al. New insights into the interaction between pyrrolyl diketoacids and HIV-1 integrase active site and comparison with RNase H. Antiviral Res. 2016;134:236-243 pubmed publisher
    ..mutagenesis studies showing that the DKA pyrrolyl scaffold primarily interacts with the IN amino residues P145, Q146 and Q148...
  14. Ma J, Kelich J, Junod S, Yang W. Super-resolution mapping of scaffold nucleoporins in the nuclear pore complex. J Cell Sci. 2017;130:1299-1306 pubmed publisher
    ..Our data reveal both the radial and axial spatial distributions for Pom121, Nup37 and Nup35 and provide evidence for their copy numbers of 8, 32 and 16, respectively, per NPC...
  15. Nordstrom T, Pandey M, Calcutt A, Powell J, Phillips Z, Yeung G, et al. Enhancing Vaccine Efficacy by Engineering a Complex Synthetic Peptide To Become a Super Immunogen. J Immunol. 2017;199:2794-2802 pubmed publisher
    ..Peptide variants of p145, a conserved helical peptide epitope from the M protein of Streptococcus pyogenes, were designed by ..
  16. Tsai T, Wang H, Hung C, Lin P, Lee Y, Chen H, et al. Wheat germ agglutinin-induced paraptosis-like cell death and protective autophagy is mediated by autophagy-linked FYVE inhibition. Oncotarget. 2017;8:91209-91222 pubmed publisher
    ..binds cell surface glycoproteins and disrupts nuclear pore complex function through its interaction with POM121. Our data indicate WGA induces paraptosis-like cell death without caspase activation...
  17. Anderson D, Vargas J, Hsiao J, Hetzer M. Recruitment of functionally distinct membrane proteins to chromatin mediates nuclear envelope formation in vivo. J Cell Biol. 2009;186:183-91 pubmed publisher
    ..membrane proteins such as lamin B receptor (LBR), MAN1, Lap2beta, and the trans-membrane nucleoporins Ndc1 and POM121 drive the spreading of ER membranes into the emerging NE via their capacity to bind chromatin in a collaborative ..
  18. Kralt A, Jagalur N, van den Boom V, Lokareddy R, Steen A, Cingolani G, et al. Conservation of inner nuclear membrane targeting sequences in mammalian Pom121 and yeast Heh2 membrane proteins. Mol Biol Cell. 2015;26:3301-12 pubmed publisher
    ..This NLS of Pom121 adapts a similar fold as the NLS of Heh2 when transport factor bound and rescues the subcellular localization and ..
  19. Fouchier R, Meyer B, Simon J, Fischer U, Albright A, Gonzalez Scarano F, et al. Interaction of the human immunodeficiency virus type 1 Vpr protein with the nuclear pore complex. J Virol. 1998;72:6004-13 pubmed
    ..These findings not only demonstrate that Vpr harbors a bona fide NLS but also raise the possibility that one (or more) of Vpr's functions may take place at the NPC. ..
  20. Talamas J, Hetzer M. POM121 and Sun1 play a role in early steps of interphase NPC assembly. J Cell Biol. 2011;194:27-37 pubmed publisher
    ..In this paper, we provide evidence that the transmembrane nucleoporin (Nup), POM121, but not the Nup107-160 complex, is present at new pore assembly sites at a time that coincides with inner nuclear ..
  21. Kihlmark M, Imreh G, Hallberg E. Sequential degradation of proteins from the nuclear envelope during apoptosis. J Cell Sci. 2001;114:3643-53 pubmed
    We have produced new antibodies specific for the integral pore membrane protein POM121. Using these antibodies we show that during apoptosis POM121 becomes proteolytically degraded in a caspase-dependent manner...
  22. Kipersztok S, Osawa G, Liang L, Modi W, Dean J. POM-ZP3, a bipartite transcript derived from human ZP3 and a POM121 homologue. Genomics. 1995;25:354-9 pubmed
    Human POM-ZP3 is a novel bipartite RNA transcript that is derived from a gene homologous to rat POM121 (a nuclear pore membrane protein) and ZP3 (a sperm receptor ligand in the zona pellucida)...
  23. Funakoshi T, Clever M, Watanabe A, Imamoto N. Localization of Pom121 to the inner nuclear membrane is required for an early step of interphase nuclear pore complex assembly. Mol Biol Cell. 2011;22:1058-69 pubmed publisher
    ..Functional domain analysis of Pom121 showed that its nuclear localization signals, which bind to importin ? via importin ? and likely function with ..
  24. Franks T, Benner C, Narvaiza I, Marchetto M, Young J, Malik H, et al. Evolution of a transcriptional regulator from a transmembrane nucleoporin. Genes Dev. 2016;30:1155-71 pubmed publisher
    ..Here we identify a widely expressed variant of the transmembrane nucleoporin (Nup) Pom121 (named sPom121, for "soluble Pom121") that arose by genomic rearrangement before the divergence of hominoids...