POLR2F

Summary

Gene Symbol: POLR2F
Description: polymerase (RNA) II (DNA directed) polypeptide F
Alias: HRBP14.4, POLRF, RPABC2, RPB14.4, RPB6, DNA directed RNA polymerase II 14.4 kda polypeptide, DNA-directed RNA polymerase II subunit F, DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide, DNA-directed RNA polymerases I, II, and III subunit RPABC2, RNA Polymerase II subunit 14.4 kD, RNA polymerases I, II, and III subunit ABC2, RPABC14.4, RPB6 homolog, RPC15
Species: human

Top Publications

  1. ncbi The fission yeast Schizosaccharomyces pombe rpb6 gene encodes the common phosphorylated subunit of RNA polymerase and complements a mutation in the corresponding gene of Saccharomyces cerevisiae
    G V Shpakovski
    Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, MD 20892
    Gene 147:63-9. 1994
  2. pmc Loss of the Rpb4/Rpb7 subcomplex in a mutant form of the Rpb6 subunit shared by RNA polymerases I, II, and III
    Qian Tan
    Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854 5635, USA
    Mol Cell Biol 23:3329-38. 2003
  3. pmc HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription
    Nanhai He
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 38:428-38. 2010
  4. ncbi A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA
    P Wei
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 1099, USA
    Cell 92:451-62. 1998
  5. pmc The HIV-1 virion-associated protein vpr is a coactivator of the human glucocorticoid receptor
    T Kino
    Section on Pediatric Endocrinology, Developmental Endocrinology Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    J Exp Med 189:51-62. 1999
  6. ncbi Inhibition of HIV-1 virus replication using small soluble Tat peptides
    Emmanuel Agbottah
    Department of Biochemistry and Molecular Biology, The George Washington University School of Medicine, Washington DC 20037, USA
    Virology 345:373-89. 2006
  7. pmc CDK9 autophosphorylation regulates high-affinity binding of the human immunodeficiency virus type 1 tat-P-TEFb complex to TAR RNA
    M E Garber
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Mol Cell Biol 20:6958-69. 2000
  8. pmc HIV-1 tat transcriptional activity is regulated by acetylation
    R E Kiernan
    Laboratoire de Virologie Moléculaire et Transfert de Gène, Institut de Genetique Humaine, UPR1142 Montpellier, 34396, France
    EMBO J 18:6106-18. 1999
  9. ncbi HIV-1 Tat interaction with RNA polymerase II C-terminal domain (CTD) and a dynamic association with CDK2 induce CTD phosphorylation and transcription from HIV-1 promoter
    Longwen Deng
    Department of Biochemistry and Molecular Biology, George Washington University Medical Center, Washington, D C 20037, USA
    J Biol Chem 277:33922-9. 2002
  10. pmc A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription
    C A Parada
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021, USA
    EMBO J 18:3688-701. 1999

Research Grants

  1. DISSECTION OF YEAST RNA POLYMERASE II FUNCTION
    Nancy Woychik; Fiscal Year: 2001
  2. Multifunctional class I transcription in T. brucei
    ARTHUR GUNZL; Fiscal Year: 2010
  3. SIGNIFICANCE OF LOW LEVEL VIREMIA IN PATIENTS ON HAART
    Robert Siliciano; Fiscal Year: 2006
  4. Latent Viral Reservoirs in HIV 1 Infection
    Robert Siliciano; Fiscal Year: 2007
  5. Intermolecular cooperativity in the action of antiviral agents
    Robert F Siliciano; Fiscal Year: 2010
  6. Latent Viral Reservoirs in HIV 1 Infection
    Robert F Siliciano; Fiscal Year: 2010
  7. CYTOLYTIC T CELL RESPONSES IN AIDS VACCINE RECIPIENTS
    Robert Siliciano; Fiscal Year: 1993
  8. CYTOLYTIC T CELL RESPONSE IN AIDS VACCINE RECIPENTS
    Robert Siliciano; Fiscal Year: 2001
  9. LATENT VIRAL RESERVOIRS IN HIV1 INFECTION
    Robert Siliciano; Fiscal Year: 2002
  10. Latent Viral Reservoirs in HIV 1 Infection
    Robert Siliciano; Fiscal Year: 2009

Scientific Experts

  • T Okamoto
  • Robert Siliciano
  • Andrew P Rice
  • K T Jeang
  • T Kino
  • David Harrich
  • Sergei Nekhai
  • Emmanuel Agbottah
  • Nancy Woychik
  • ARTHUR GUNZL
  • A I Garrido-Godino
  • Hyunsuk Suh
  • D Ivanov
  • C Suñé
  • M Krol
  • Nanhai He
  • M Zhou
  • Kalle T Rytkönen
  • Young Kyeung Kim
  • A Ishihama
  • Cyril F Bourgeois
  • A Ishiguro
  • Anna G Antonacopoulou
  • Sara Devaux
  • F Navarro
  • Miguel Stevens
  • Tu N Nguyen
  • R G Roeder
  • R B Gaynor
  • M Kimura
  • I Agostini
  • H Xiao
  • M E Garber
  • Betty Poon
  • Katrin Kaehlcke
  • Qian Tan
  • Tricia H Hogan
  • Anna V Kuznetsova
  • Y Liu
  • H Sakurai
  • M A Garcia-Blanco
  • Mark J Churcher
  • Chao Zhou
  • Jonathan Karn
  • K A Jones
  • Guadalupe Nogues
  • Longwen Deng
  • Ping Hu
  • M C García-López
  • Stephen Buratowski
  • Luis M Soares
  • L F García-Martínez
  • Dane Z Hazelbaker
  • K Fujinaga
  • T P Cujec
  • J Acker
  • G V Shpakovski
  • J F Briand
  • A Marcello
  • Y H Ping
  • L Minakhin
  • B M Peterlin
  • M Vigneron
  • B P Schlegel
  • Erica H Leder
  • Nevan J Krogan
  • J Skierski
  • R E Morty
  • T Motyl
  • E Jones
  • Min Liu
  • F Kashanchi
  • N E Thompson
  • M Kaarbo
  • Mikko Nikinmaa
  • Grant Williams-Pritchard
  • K M Pawłowski
  • M Ugorski
  • B E Sawaya
  • Yuhua Xue
  • A Majewska
  • Joanne Hsu
  • P Cramer
  • Tom Alber
  • P Turowski
  • Qiang Zhou
  • Gillian M C Renshaw
  • J Polanska
  • J N Brady
  • Alma Burlingame

Detail Information

Publications86

  1. ncbi The fission yeast Schizosaccharomyces pombe rpb6 gene encodes the common phosphorylated subunit of RNA polymerase and complements a mutation in the corresponding gene of Saccharomyces cerevisiae
    G V Shpakovski
    Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, MD 20892
    Gene 147:63-9. 1994
    A single-copy gene, homologous to the RPB6 gene from Saccharomyces cerevisiae, encoding a small phosphorylated subunit common to all three forms of nuclear DNA-dependent RNA polymerase was isolated from the fission yeast ..
  2. pmc Loss of the Rpb4/Rpb7 subcomplex in a mutant form of the Rpb6 subunit shared by RNA polymerases I, II, and III
    Qian Tan
    Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854 5635, USA
    Mol Cell Biol 23:3329-38. 2003
    We have identified a conditional mutation in the shared Rpb6 subunit, assembled in RNA polymerases I, II, and III, that illuminated a new role that is independent of its assembly function...
  3. pmc HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription
    Nanhai He
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 38:428-38. 2010
    ..The ability of Tat to enable two different classes of elongation factors to cooperate and coordinate their actions on the same polymerase enzyme explains why Tat is such a powerful activator of HIV-1 transcription...
  4. ncbi A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA
    P Wei
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 1099, USA
    Cell 92:451-62. 1998
    ..Moreover, overexpression of human cyclin T rescues Tat activity in nonpermissive rodent cells. We propose that Tat directs cyclin T-CDK9 to RNAPII through cooperative binding to TAR RNA...
  5. pmc The HIV-1 virion-associated protein vpr is a coactivator of the human glucocorticoid receptor
    T Kino
    Section on Pediatric Endocrinology, Developmental Endocrinology Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    J Exp Med 189:51-62. 1999
    ..The glucocorticoid coactivator activity of Vpr may contribute to increased tissue glucocorticoid sensitivity in the absence of hypercortisolism and to the pathogenesis of AIDS...
  6. ncbi Inhibition of HIV-1 virus replication using small soluble Tat peptides
    Emmanuel Agbottah
    Department of Biochemistry and Molecular Biology, The George Washington University School of Medicine, Washington DC 20037, USA
    Virology 345:373-89. 2006
    ..Finally, we show that these peptides do not allow loading of the catalytic domain of the cdk/cyclin complex onto the HIV-1 promoter in vivo...
  7. pmc CDK9 autophosphorylation regulates high-affinity binding of the human immunodeficiency virus type 1 tat-P-TEFb complex to TAR RNA
    M E Garber
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Mol Cell Biol 20:6958-69. 2000
    ..Taken together, these results demonstrate that CDK9 phosphorylation is required for high-affinity binding of Tat-P-TEFb to TAR RNA and that the state of P-TEFb phosphorylation may regulate Tat transactivation in vivo...
  8. pmc HIV-1 tat transcriptional activity is regulated by acetylation
    R E Kiernan
    Laboratoire de Virologie Moléculaire et Transfert de Gène, Institut de Genetique Humaine, UPR1142 Montpellier, 34396, France
    EMBO J 18:6106-18. 1999
    ..These data suggest that acetylation of Tat regulates two discrete and functionally critical steps in transcription, binding to an RNAP II CTD-kinase and release of Tat from TAR RNA...
  9. ncbi HIV-1 Tat interaction with RNA polymerase II C-terminal domain (CTD) and a dynamic association with CDK2 induce CTD phosphorylation and transcription from HIV-1 promoter
    Longwen Deng
    Department of Biochemistry and Molecular Biology, George Washington University Medical Center, Washington, D C 20037, USA
    J Biol Chem 277:33922-9. 2002
    ..We suggest that CDK2 is part of a transcription complex that is required for Tat-dependent transcription and that interaction of Tat with CTD and a dynamic association of Tat with CDK2/cyclin E stimulated CTD phosphorylation by CDK2...
  10. pmc A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription
    C A Parada
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021, USA
    EMBO J 18:3688-701. 1999
    ..Our results indicate that Tat-SF is a Tat cofactor-containing RNA Pol II complex whose recruitment to the promoter provides elongation factors important for Tat-enhanced HIV-1 transcription following TAR RNA synthesis...
  11. pmc Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor
    C H Herrmann
    Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030 3498
    J Virol 69:1612-20. 1995
    ..Taken together, these results imply that TAK is a very promising candidate for a cellular factor that mediates Tat transactivation...
  12. pmc Domains in the SPT5 protein that modulate its transcriptional regulatory properties
    D Ivanov
    Division of Hematology Oncology, Department of Medicine, Harold Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas 75235 8594, USA
    Mol Cell Biol 20:2970-83. 2000
    ..These results suggest that C-terminal repeats in SPT5, like those in the RNA polymerase II C-terminal domain, are sites for P-TEFb phosphorylation and function in modulating its transcriptional elongation properties...
  13. ncbi Transcription factor TFIIH components enhance the GR coactivator activity but not the cell cycle-arresting activity of the human immunodeficiency virus type-1 protein Vpr
    Tomoshige Kino
    Pediatric and Reproductive Endocrinology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Biochem Biophys Res Commun 298:17-23. 2002
    ..These findings suggest that TFIIH participates in Vpr's GR coactivating activity, at a step beyond its interaction with p300/CBP...
  14. pmc The ability of positive transcription elongation factor B to transactivate human immunodeficiency virus transcription depends on a functional kinase domain, cyclin T1, and Tat
    K Fujinaga
    Departments of Medicine, Microbiology, and Immunology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California 94143 0703, USA
    J Virol 72:7154-9. 1998
    ..Moreover, P-TEFb binds to TAR only in the presence of Tat. We conclude that Tat-P-TEFb complexes bind to TAR, where CDK9 modifies RNA polymerase II for the efficient copying of the viral genome...
  15. ncbi The human immunodeficiency virus type 1 Vpr transactivator: cooperation with promoter-bound activator domains and binding to TFIIB
    I Agostini
    INSERM U372, Pathogénie des infections à lentivirus BP 178, Marseille, France
    J Mol Biol 261:599-606. 1996
    ..We demonstrated that the portion of Vpr ranging from amino acids 15 to 77 interacts specifically with the basal transcription factor TFIIB. Also, our data indicated that the N-terminal domain of TFIIB is required for the interaction...
  16. ncbi Activation of transcription by HIV-1 Tat protein tethered to nascent RNA through another protein
    C Southgate
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    Nature 345:640-2. 1990
    ..Our results further suggest that cellular proteins that bind specifically to TAR RNA or TAR DNA may not be essential for Tat-responsiveness...
  17. pmc Human immunodeficiency virus type-1 Tat is an integral component of the activated transcription-elongation complex
    N J Keen
    Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    Proc Natl Acad Sci U S A 93:2505-10. 1996
    ..We conclude that Tat and cellular cofactors become attached to the transcription complex during its transit through TAR...
  18. ncbi Transcriptional and post-transcriptional regulation of HIV-1 gene expression: role of cellular factors for Tat and Rev
    Sergei Nekhai
    Center for Sickle Cell Disease and Department of Biochemistry and Molecular Biolology, Howard University, NW Washington, DC 20059, USA
    Future Microbiol 1:417-26. 2006
    ..Rev primarily functions to export unspliced and partially spliced viral RNAs from the nucleus into the cytoplasm. For this activity, Rev cooperates with cellular transport protein CRM1 and RNA helicases DDX1 and DDX3, amongst others...
  19. ncbi Cooperative interaction between HIV-1 regulatory proteins Tat and Vpr modulates transcription of the viral genome
    B E Sawaya
    Center for Neurovirology and Cancer Biology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania 19122, USA
    J Biol Chem 275:35209-14. 2000
    ..Moreover identification of R73S mutant of Vpr provides a new therapeutic avenue for controlling HIV-1 gene transcription and replication in the infected cells...
  20. ncbi The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention
    Miguel Stevens
    Rega Institute for Medical Research, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Med Res Rev 26:595-625. 2006
    ..As such, targeting of Tat protein (and/or cellular cofactors) provide an interesting perspective for therapeutic intervention in the HIV replicative cycle and may afford lifetime control of the HIV infection...
  21. pmc HIV-1 Tat-associated RNA polymerase C-terminal domain kinase, CDK2, phosphorylates CDK7 and stimulates Tat-mediated transcription
    Sergei Nekhai
    Department of Biochemistry and Molecular Biology, The George Washington University, School of Medicine, 2300 Eye Street N W, Washington, DC 20037, USA
    Biochem J 364:649-57. 2002
    ..They are also consistent with the observed cell-cycle-specific induction of viral gene transactivation...
  22. pmc Spt5 cooperates with human immunodeficiency virus type 1 Tat by preventing premature RNA release at terminator sequences
    Cyril F Bourgeois
    MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, England
    Mol Cell Biol 22:1079-93. 2002
    ..This novel biochemical function of Spt5 is analogous to the function of NusG, an elongation factor found in Escherichia coli that enhances RNA polymerase stability on templates and shows sequence similarity to Spt5...
  23. ncbi Multiple modes of transcriptional regulation by the HIV-1 Tat transactivator
    A Marcello
    Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy
    IUBMB Life 51:175-81. 2001
    ....
  24. pmc Tat modifies the activity of CDK9 to phosphorylate serine 5 of the RNA polymerase II carboxyl-terminal domain during human immunodeficiency virus type 1 transcription
    M Zhou
    Virus Tumor Biology Section, LRBGE, Division of Basic Sciences, National Cancer Institute, Bethesda, Maryland 20892, USA
    Mol Cell Biol 20:5077-86. 2000
    ..These studies suggest that the ability of Tat to increase transcriptional elongation may be due to its ability to modify the substrate specificity of the CDK9 complex...
  25. ncbi The HIV-1 Vpr co-activator induces a conformational change in TFIIB
    I Agostini
    INSERM U372, Marseille, France
    FEBS Lett 450:235-9. 1999
    ..Our data show a correlation between the ability of Vpr-mutated proteins to stimulate transcription and their ability to induce a conformational change in TFIIB, indicating a functional relevance of the Vpr-TFIIB interaction...
  26. ncbi Direct evidence that HIV-1 Tat stimulates RNA polymerase II carboxyl-terminal domain hyperphosphorylation during transcriptional elongation
    C Isel
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    J Mol Biol 290:929-41. 1999
    ..We conclude that activation of the CDK9 kinase, leading to CTD phosphorylation, occurs only in elongation complexes that have transcribed through the Tat-recognition element, TAR RNA...
  27. pmc Phosphorylation of the RNA polymerase II carboxyl-terminal domain by CDK9 is directly responsible for human immunodeficiency virus type 1 Tat-activated transcriptional elongation
    Young Kyeung Kim
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, United Kingdom
    Mol Cell Biol 22:4622-37. 2002
    ..We conclude that phosphorylation of the RNA polymerase II CTD by CDK9 enhances transcription elongation directly...
  28. ncbi A bimolecular mechanism of HIV-1 Tat protein interaction with RNA polymerase II transcription elongation complexes
    Chao Zhou
    Chemical Biology Program, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605 2324, USA
    J Mol Biol 320:925-42. 2002
    ..These findings suggest that two Tat molecules are involved in performing various functions during a single round of HIV-1 mRNA synthesis...
  29. pmc A human primary T-lymphocyte-derived human immunodeficiency virus type 1 Tat-associated kinase phosphorylates the C-terminal domain of RNA polymerase II and induces CAK activity
    S Nekhai
    Department of Biochemistry and Molecular Biology, George Washington University School of Medicine, Washington, D C 20037, USA
    J Virol 71:7436-41. 1997
    ..Importantly, the Tat-associated kinase markedly induced CAK. We suggest that the mechanism of Tat-mediated processive transcription of the HIV-1 promoter includes a Tat-associated CAK activator...
  30. ncbi HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors
    H Kato
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021
    Genes Dev 6:655-66. 1992
    ..We propose the hypothesis that Tat acts as a processivity factor on RNA polymerase II in an analogous manner to TFIIF...
  31. ncbi HIV-1 Vpr binding to HIV-1 LTR C/EBP cis-acting elements and adjacent regions is sequence-specific
    Tricia H Hogan
    Department of Microbiology and Immunology, The Pennsylvania State University, College of Medicine, H107, 500 University Drive, P O Box 850, Hershey, PA 17033, USA
    Biomed Pharmacother 57:41-8. 2003
    ..These studies suggest that Vpr may regulate the interaction of members of the C/EBP transcription factor family with the viral LTR...
  32. ncbi Requirements for RNA polymerase II carboxyl-terminal domain for activated transcription of human retroviruses human T-cell lymphotropic virus I and HIV-1
    R F Chun
    Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Biol Chem 271:27888-94. 1996
    ..Taken together, these observations address mechanistic corollaries between activators with(out) a linked CTD kinase and regulated transcription by RNA polymerase II moieties with(out) a CTD...
  33. pmc Purification of a Tat-associated kinase reveals a TFIIH complex that modulates HIV-1 transcription
    L F García-Martínez
    Department of Medicine, University of Texas Southwestern Medical Center, Dallas 75235 8594, USA
    EMBO J 16:2836-50. 1997
    ..These results define a cellular kinase complex whose activity is modulated by Tat to result in activation of HIV-1 trancription...
  34. pmc Human immunodeficiency virus type 1 (HIV-1) Vpr enhances expression from unintegrated HIV-1 DNA
    Betty Poon
    Department of Microbiology, David Geffen School of Medicine at UCLA, UCLA AIDS Institute and Jonsson Comprehensive CAncer Center, Los Angeles, California 90095, USA
    J Virol 77:3962-72. 2003
    ..These results attribute a new function to HIV-1 Vpr and implicate Vpr as a critical component in expression from unintegrated HIV-1 DNA...
  35. pmc CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription
    C Suñé
    Department of Molecular Cancer Biology, Levine Science Research Center, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 17:6029-39. 1997
    ..Furthermore, we found that functional Tat associates with the holoenzyme whereas activation-deficient Tat mutants do not. Thus, we propose that Tat action is transduced via an RNA polymerase II holoenzyme that contains CA150...
  36. ncbi DSIF and NELF interact with RNA polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation
    Y H Ping
    Department of Pharmacology, Robert Wood Johnson Medical School, and Molecular Biosciences Graduate Program at Rutgers University, Piscataway, New Jersey 08854, USA
    J Biol Chem 276:12951-8. 2001
    ..These findings reveal a molecular mechanism for the negative and positive regulation of transcriptional elongation at the HIV-1 promoter...
  37. pmc Human and rodent transcription elongation factor P-TEFb: interactions with human immunodeficiency virus type 1 tat and carboxy-terminal domain substrate
    Y Ramanathan
    Department of Biochemistry and Molecular Biology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
    J Virol 73:5448-58. 1999
    ..We suggest a model in which Tat first interacts with P-TEFb to form the TAK complex that engages with TAR RNA and the elongating transcription complex, resulting in hyperphosphorylation of the CTD on serine 5 residues...
  38. ncbi Genomic structure of the RNA polymerase II small subunit (hRPB14.4) locus (POLRF) and mapping to 22q13.1 by sequence identity
    C Pusch
    Institute of Anthropology and Human Genetics, University of Tubingen, Tubingen, Germany
    Genomics 34:440-2. 1996
  39. pmc The Rpb6 subunit of fission yeast RNA polymerase II is a contact target of the transcription elongation factor TFIIS
    A Ishiguro
    School of Life Science, Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Mol Cell Biol 20:1263-70. 2000
    The Rpb6 subunit of RNA polymerase II is one of the five subunits common to three forms of eukaryotic RNA polymerase...
  40. pmc Partners of Rpb8p, a small subunit shared by yeast RNA polymerases I, II and III
    J F Briand
    Service de Biochimie and Génétique Moléculaire, CEA Saclay, F 91191 Gif sur Yvette, France
    Mol Cell Biol 21:6056-65. 2001
    ..Rpb6p is phosphorylated at its N-terminal Ser2, but an alanyl replacement at this position still complements an rpb6-Delta null allele. A two-hybrid interaction also occurs between Rpb8p and the product of orphan gene YGR089w...
  41. ncbi Isolation and characterisation of a chick cDNA encoding the RNA polymerase common subunit RPB6
    M Kaarbo
    School of Biomolecular and Biomedical Science, Griffith University, Nathan, Queensland, Australia
    DNA Seq 11:155-62. 2000
    The RPB6 cDNA of chicken, encoding one of the small subunits common to all three nuclear DNA-dependent RNA polymerases, has been isolated from an expression cDNA library by screening with a differential display derived probe, representing ..
  42. doi Transcriptomic signature of cell lines isolated from canine mammary adenocarcinoma metastases to lungs
    M Krol
    Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences SGGW, ul Nowoursynowska 159, 02 776 Warsaw, Poland
    J Appl Genet 51:37-50. 2010
    ..apoptosis (GHSR, RASSF1, ARF1GAP, WDR74, SMOC2, SFRP4, DIAPH1, FSCN1, ALX4, SNX15, PLD2, WNT7B, POU6F2, NKG7, and POLR2F)...
  43. ncbi The HIV-1 Tat team gets bigger
    Andrew P Rice
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA
    Cell Host Microbe 7:179-81. 2010
    ..Now, Pagans and colleagues report that the lysine methyltransferase Set7/9-KMT7 associates with Tat to stimulate RNA polymerase II elongation of the integrated provirus. Set7/9-KMT7 also methylates Tat, and this enhances Tat function...
  44. pmc HIV latency
    Robert F Siliciano
    Department of Medicine, Johns Hopkins University School of Medicine, Howard Hughes Medical Institute, Baltimore, Maryland 21205, USA
    Cold Spring Harb Perspect Med 1:a007096. 2011
    ..Several approaches are under exploration for reactivating latent virus with the hope that this will allow elimination of the latent reservoir...
  45. pmc Correct assembly of RNA polymerase II depends on the foot domain and is required for multiple steps of transcription in Saccharomyces cerevisiae
    A I Garrido-Godino
    Departamento de Biologia Experimental, Facultad de Ciencias Experimentales, Universidad de Jaen, Jaen, Spain
    Mol Cell Biol 33:3611-26. 2013
    ..pol II is crucial for the assembly and stability of the complex, by ensuring the correct association of Rpb1 with Rpb6 and of the dimer Rpb4-Rpb7 (Rpb4/7)...
  46. ncbi Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei
    Tu N Nguyen
    Department of Genetics and Developmental Biology and Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3301, USA
    Mol Biochem Parasitol 149:27-37. 2006
    ..of trypanosomatid genomes have revealed 13 potential RNA pol I subunits which include two paralogous sets of RPB5, RPB6, and RPB10. Here, we analyzed a cDNA library prepared from procyclic insect form T...
  47. pmc The C-terminal domain of Rpb1 functions on other RNA polymerase II subunits
    Hyunsuk Suh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 51:850-8. 2013
    ..To address this question, CTD was transferred to other RNApII subunits. Fusions to Rpb4 or Rpb6, two RNApII subunits located near the original position of CTD, support viability in a strain carrying a truncated ..
  48. pmc Functional substitution of an essential yeast RNA polymerase subunit by a highly conserved mammalian counterpart
    K McKune
    Roche Institute of Molecular Biology, Nutley, New Jersey 07110
    Mol Cell Biol 14:4155-9. 1994
    We isolated the cDNA encoding the homolog of the Saccharomyces cerevisiae nuclear RNA polymerase common subunit RPB6 from hamster CHO cells...
  49. pmc Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits
    Ping Hu
    Graduate Program of Molecular and Cellular Biology, State University of New York at Stony Brook, Stony Brook, New York 11794, USA
    Mol Cell Biol 22:8044-55. 2002
    ..Our results provide a characterization of human RNA polymerase III and show that the RPC5 subunit is essential for transcription...
  50. pmc von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination
    Anna V Kuznetsova
    Department of Molecular and Cellular Physiology, Children s Hospital Research Foundation, University of Cincinnati College of Medicine, Cincinnati, OH 45267 0576, USA
    Proc Natl Acad Sci U S A 100:2706-11. 2003
    ..By using computer modeling, we identified regions of Rpb1 and the adjacent subunit 6 of RNA polymerase II (Rpb6) that share sequence and structural similarity with the domain of hypoxia-inducible transcription factor 1 alpha (..
  51. ncbi Interactions between the human RNA polymerase II subunits
    J Acker
    Institut de Genetique et de Biologie Moleculaire et Cellulaire CNRS INSERM ULP, F 67404 Illkirch Cedex C U de Strasbourg, France
    J Biol Chem 272:16815-21. 1997
    ..These subunits, which are able to homodimerize and to interact, may constitute the nucleation center for polymerase assembly, by providing a large interface to most of the other subunits...
  52. ncbi Acetylation of Tat defines a cyclinT1-independent step in HIV transactivation
    Katrin Kaehlcke
    Deutsches Krebsforschungszentrum, D 69120 Heidelberg, Germany
    Mol Cell 12:167-76. 2003
    ..We propose that Tat acetylation may help in dissociating the Tat cofactor CyclinT1 from TAR RNA and serve to transfer Tat onto the elongating RNA polymerase II...
  53. ncbi Sequence analysis of closely related retrotransposon families from fission yeast
    D C Weaver
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205
    Gene 131:135-9. 1993
    ..pombe rpb6 gene...
  54. ncbi Isolation and characterization of monoclonal antibodies directed against subunits of human RNA polymerases I, II, and III
    E Jones
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, United Kingdom
    Exp Cell Res 254:163-72. 2000
    ..We now describe eight different monoclonal antibodies that react specifically with RPB6 (also known as RPA20, RPB14...
  55. pmc Diversification of function by different isoforms of conventionally shared RNA polymerase subunits
    Sara Devaux
    Institute for Molecular Biology and Medicine, Universite Libre de Bruxelles, 6041 Gosselies, Belgium
    Mol Biol Cell 18:1293-301. 2007
    ..At the core of each complex is a set of 12 highly conserved subunits of which five--RPB5, RPB6, RPB8, RPB10, and RPB12--are thought to be common to all three polymerase classes...
  56. pmc Halobacterial S9 operon contains two genes encoding proteins homologous to subunits shared by eukaryotic RNA polymerases I, II, and III
    K McKune
    Roche Institute of Molecular Biology, Nutley, New Jersey 07110
    J Bacteriol 176:4754-6. 1994
    ..We have discovered that two of the subunits shared by the three nuclear RNA polymerases in the yeast Saccharomyces cerevisiae, RPB6 and RPB10, have counterparts among the Archaea.
  57. ncbi POLR2F, ATP6V0A1 and PRNP expression in colorectal cancer: new molecules with prognostic significance?
    Anna G Antonacopoulou
    Clinical Oncology Laboratory, Medical School, University of Patras, Patras, Greece
    Anticancer Res 28:1221-7. 2008
    DNA-directed RNA polymerase II subunit F (POLR2F), a subunit of the V0 domain of the vacuolar ATPase (ATP6V0A1) and the prion protein (PRNP) are molecules of potential importance in carcinogenesis and targeted cancer therapy...
  58. ncbi A 14.4 KDa acidic subunit of human RNA polymerase II with a putative leucine-zipper
    J Acker
    Laboratoire de Génétique Moléculaire des Eucaryotes CNRS, Unité 184 de Biologie Moléculaire et de Génétique INSERM, Institut de Chime Biologique de la Faculté de Médecine, Strasbourg, France
    DNA Seq 4:329-31. 1994
    ..and compared to that of the homologous subunit of Saccharomyces cerevisiae polymerase (ABC23, encoded by the RPB6/RPO26 gene)...
  59. pmc Elasmobranch qPCR reference genes: a case study of hypoxia preconditioned epaulette sharks
    Kalle T Rytkönen
    Division of Genetics and Physiology, Department of Biology, University of Turku, FI 20014 Turku, Finland
    BMC Mol Biol 11:27. 2010
    ..Here we examined the suitability of 9 reference candidates from various functional categories after a single hypoxic insult or after hypoxia preconditioning in epaulette shark (Hemiscyllium ocellatum)...
  60. ncbi Subunits shared by eukaryotic nuclear RNA polymerases
    N A Woychik
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142
    Genes Dev 4:313-23. 1990
    ..The Saccharomyces cerevisiae genes that encode these subunits (RPB5, RPB6, and RPB8) were isolated and sequenced, and their transcriptional start sites were deduced...
  61. pmc RNA polymerase II subunit composition, stoichiometry, and phosphorylation
    P A Kolodziej
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142
    Mol Cell Biol 10:1915-20. 1990
    ..Immunoprecipitation from 32P-labeled cell extracts revealed that three of the subunits, RPB1, RPB2, and RPB6, are phosphorylated in vivo...
  62. pmc Genes encoding transcription factor IIIA and the RNA polymerase common subunit RPB6 are divergently transcribed in Saccharomyces cerevisiae
    N A Woychik
    Department of Gene Regulation, Roche Institute of Molecular Biology, Nutley, NJ 07110
    Proc Natl Acad Sci U S A 89:3999-4003. 1992
    The gene encoding Saccharomyces cerevisiae transcription factor TFIIIA has been found adjacent to RPB6, a gene that specifies a subunit shared by nuclear RNA polymerases...
  63. ncbi Will diverse Tat interactions lead to novel antiretroviral drug targets?
    David Harrich
    HIV 1 Molecular Virology Laboratory, Division of Immunology and Infectious Diseases, Queensland Institute of Medical Research, Royal Brisbane Hospital Post Office, Brisbane 4029, QLD, Australia
    Curr Drug Targets 7:1595-606. 2006
    ..Nevertheless, Tat remains an attractive, virus-specific molecule and detailed understanding of specific protein interaction holds promise for future drug discovery...
  64. ncbi Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II
    H Sakurai
    National Institute of Genetics, Department of Molecular Genetics, Shizuoka, Japan
    Gene 196:165-74. 1997
    ..Later, other groups isolated the genes for Rpb6 and Rpb12 and cDNA for Rpb10...
  65. pmc TIP30 has an intrinsic kinase activity required for up-regulation of a subset of apoptotic genes
    H Xiao
    Laboratory of Biochemistry, The Rockefeller University, New York, NY 10021, USA
    EMBO J 19:956-63. 2000
    ..These data demonstrate a molecular mechanism for TIP30/CC3 function and suggest a novel pathway for regulating apoptosis...
  66. pmc Bacterial RNA polymerase subunit omega and eukaryotic RNA polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly
    L Minakhin
    Waksman Institute, Department of Genetics, Department of Chemistry and Howard Hughes Medical Institute, Rutgers, The State University, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 98:892-7. 2001
    ..The role of omega has been unclear. We show that omega is homologous in sequence and structure to RPB6, an essential subunit shared in eukaryotic RNAP I, II, and III...
  67. ncbi Intracellular contents and assembly states of all 12 subunits of the RNA polymerase II in the fission yeast Schizosaccharomyces pombe
    M Kimura
    National Institute of Genetics, Department of Molecular Genetics, Mishima, Shizuoka, Japan
    Eur J Biochem 268:612-9. 2001
    ..yeast Schizosaccharomyces pombe is composed of 12 different polypeptides, Rpb1 to Rpb12, of which five, Rpb5, Rpb6, Rpb8, Rpb10 and Rpb12, are shared among three forms of the RNA polymerase...
  68. pmc Transcriptional cofactor CA150 regulates RNA polymerase II elongation in a TATA-box-dependent manner
    C Suñé
    Departments of Pharmacology and Cancer Biology, Levine Science Research Center, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 19:4719-28. 1999
    ..We discuss the data in terms of the involvement of CA150 in the regulation of Tat-activated HIV-1 gene expression. In addition, we also provide evidence suggesting a role for CA150 in the regulation of cellular transcriptional processes...
  69. ncbi Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes
    E Kershnar
    Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 273:34444-53. 1998
    ....
  70. ncbi A serine residue in the N-terminal acidic region of rat RPB6, one of the common subunits of RNA polymerases, is exclusively phosphorylated by casein kinase II in vitro
    K Kayukawa
    Department of Biology, Faculty of Science, Chiba University and CREST Japan Science and Technology Corporation, 1 33 Yayoi cho, Inage Ku, Chiba 263 8522, Japan
    Gene 234:139-47. 1999
    b>RPB6 is one of the common subunits of all eukaryotic RNA polymerases and is indispensable for the enzyme function. Here, we isolated a rat cDNA encoding RPB6. It contained 127 amino acid (a.a.) residues...
  71. ncbi Transcriptional control: Tat cofactors and transcriptional elongation
    K Yankulov
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Curr Biol 8:R447-9. 1998
    ..Recent results show that two cellular cyclin-dependent kinases, which phosphorylate the carboxy-terminal domain of the RNA polymerase II large subunit, contact Tat and contribute to the control of transcriptional elongation...
  72. ncbi Two large subunits of the fission yeast RNA polymerase II provide platforms for the assembly of small subunits
    A Ishiguro
    Department of Molecular Genetics, National Institute of Genetics, Shizuoka 411, Mishima, Japan
    J Mol Biol 279:703-12. 1998
    ..for a total of 19 combinations, including five combinations between small subunits, Rpb3-Rpb10, Rpb3-Rpb11, Rpb5-Rpb6, Rpb6-Rpb7 and Rpb6-Rpb8...
  73. pmc Specific binding of RNA polymerase II to the human immunodeficiency virus trans-activating region RNA is regulated by cellular cofactors and Tat
    F Wu-Baer
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235 8594, USA
    Proc Natl Acad Sci U S A 92:7153-7. 1995
    ..These results suggest that Tat may function to alter RNA polymerase II, which is paused due to its binding to HIV-1 TAR RNA with resultant stimulation of its transcriptional elongation properties...
  74. pmc BRCA1 interaction with RNA polymerase II reveals a role for hRPB2 and hRPB10alpha in activated transcription
    B P Schlegel
    Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 97:3148-53. 2000
    ..No other Pol II subunits tested inhibited activated transcription in these assays. Furthermore, hRPB10alpha, but not hRPB2, blocked Sp1-dependent activation...
  75. ncbi Cyclin T1 domains involved in complex formation with Tat and TAR RNA are critical for tat-activation
    D Ivanov
    Division of Hematology Oncology, Department of Medicine, Harold Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, 75235 8594, USA
    J Mol Biol 288:41-56. 1999
    ..These results demonstrate that cyclin T1 interactions with Tat and TAR RNA are critical for activation of HIV-1 gene expression...
  76. ncbi Architecture of RNA polymerase II and implications for the transcription mechanism
    P Cramer
    Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305 5126, USA
    Science 288:640-9. 2000
    ..A clamp on the DNA nearer the active center, formed by Rpb1, Rpb2, and Rpb6, may be locked in the closed position by RNA, accounting for the great stability of transcribing complexes...
  77. ncbi Transcriptional activators differ in their abilities to control alternative splicing
    Guadalupe Nogues
    Laboratorio de Fisiologia y Biologia Molecular, Departamento de Fisiologia, Biologia Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, C1428EHA Buenos Aires, Argentina
    J Biol Chem 277:43110-4. 2002
    ..Rapid, highly processive transcription favors EDI exon skipping, whereas slower, less processive transcription favors inclusion...
  78. pmc Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase
    J A Bokar
    Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
    RNA 3:1233-47. 1997
    ..MT-A70 also contains a long region of homology to the yeast protein SPO8, which is involved in induction of sporulation by an unknown mechanism...
  79. ncbi Tat, Tat-associated kinase, and transcription
    K T Jeang
    Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, NIH, Bethesda, MD 20892 0460, USA
    J Biomed Sci 5:24-7. 1998
    ..Here we review, in brief, the role of Tat-associated kinase in Tat-activated transcription. We discuss evidence that suggests involvement of TFIIH and/or P-TEFb...
  80. ncbi Regulatory functions of Cdk9 and of cyclin T1 in HIV tat transactivation pathway gene expression
    G Romano
    Kimmel Cancer Institute, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Cell Biochem 75:357-68. 1999
    ....
  81. pmc Trans-activation by human immunodeficiency virus Tat protein requires the C-terminal domain of RNA polymerase II
    H Okamoto
    Howard Hughes Medical Institute, Department of Medicine, University of California, San Francisco 94143 0724, USA
    Proc Natl Acad Sci U S A 93:11575-9. 1996
    ..These results suggest that effects of Tat on the processivity of RNA polymerase II require proteins that are associated with the CTD and may result in the phosphorylation of the CTD...
  82. pmc The human immunodeficiency virus Tat proteins specifically associate with TAK in vivo and require the carboxyl-terminal domain of RNA polymerase II for function
    X Yang
    Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Virol 70:4576-84. 1996
    ..These observations strengthen the proposal that the mechanism of action of Tat involves the recruitment or activation of TAK, resulting in activated transcription through phosphorylation of the CTD...
  83. ncbi Solution structure of the hRPABC14.4 subunit of human RNA polymerases
    - del Río-Portilla F
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Struct Biol 6:1039-42. 1999
    ..4 structure accounts for mutagenesis results in Saccharomyces cerevisiae and provides a structural working model for elucidating the role of this subunit in the molecular architecture and function of the human nuclear RNA polymerases...
  84. pmc The HIV transactivator TAT binds to the CDK-activating kinase and activates the phosphorylation of the carboxy-terminal domain of RNA polymerase II
    T P Cujec
    Howard Hughes Medical Institute, Department of Medicine, University of California at San Francisco, San Franscisco, California USA
    Genes Dev 11:2645-57. 1997
    ..Our data identify a cellular protein that interacts with the activation domain of Tat, demonstrate that this interaction is critical for the function of Tat, and provide a mechanism by which Tat increases the processivity of Pol II...
  85. ncbi Phosphorylation of the RAP74 subunit of TFIIF correlates with Tat-activated transcription of the HIV-1 long terminal repeat
    M Zhou
    Virus Tumor Biology Section, National Cancer Institute, Bethesda, Maryland, 20892, USA
    Virology 268:452-60. 2000
    ..Of importance, the exogenous RAP74 was rapidly phosphorylated in the presence of Tat. These results suggest that RAP74 phosphorylation is one important step, of several, in the Tat transactivation cascade...
  86. ncbi Human immunodeficiency virus type 1 Tat-dependent activation of an arrested RNA polymerase II elongation complex
    Y Liu
    Levine Science Research Center, Duke University Medical Center, Durham, North Carolina, 27710, USA
    Virology 255:337-46. 1999
    ..These data indicate that Tat can activate elongation of RNA polymerase by modifying an already elongating transcription complex. The data also suggest the possibility that Tat can interact with initiation complexes...

Research Grants39

  1. DISSECTION OF YEAST RNA POLYMERASE II FUNCTION
    Nancy Woychik; Fiscal Year: 2001
    ..focuses on the study of five of the holoenzyme components, the RNA polymerase II subunits designated RPB4, RPB5, RPB6, RPB7 and RPB9, to help identify their roles in transcription...
  2. Multifunctional class I transcription in T. brucei
    ARTHUR GUNZL; Fiscal Year: 2010
    ..RPA31, an unusual N-terminal extension domain in subunit RPA2, and a variant set of the common subunits RPB5, RPB6 and RPB10 that is specific to RNA pol I...
  3. SIGNIFICANCE OF LOW LEVEL VIREMIA IN PATIENTS ON HAART
    Robert Siliciano; Fiscal Year: 2006
    ....
  4. Latent Viral Reservoirs in HIV 1 Infection
    Robert Siliciano; Fiscal Year: 2007
    ..Together, these studies should help to elucidate how HIV-1 latency operates in vivo. ..
  5. Intermolecular cooperativity in the action of antiviral agents
    Robert F Siliciano; Fiscal Year: 2010
    ..The method incorporates a previously unappreciated factor that contributes enormously to the ability of drugs and vaccines to inhibit HIV. The goal is to facilitate the design of drugs and vaccines that maximally inhibit HIV. ..
  6. Latent Viral Reservoirs in HIV 1 Infection
    Robert F Siliciano; Fiscal Year: 2010
    ..Curing HIV infection will require new ways to eliminate this latent reservoir. The studies proposed here are designed to find new drugs that can eliminate this latent reservoir. ..
  7. CYTOLYTIC T CELL RESPONSES IN AIDS VACCINE RECIPIENTS
    Robert Siliciano; Fiscal Year: 1993
    ....
  8. CYTOLYTIC T CELL RESPONSE IN AIDS VACCINE RECIPENTS
    Robert Siliciano; Fiscal Year: 2001
    ..nonresponders. Antigen processing and presentation will then be studied using cells from non-responders. Finally the ability of HIV-1 specific CTL to lyse newly or chronically infected resting CD4 T cells will be examined. ..
  9. LATENT VIRAL RESERVOIRS IN HIV1 INFECTION
    Robert Siliciano; Fiscal Year: 2002
    ..Finally, the investigators will develop an experimental system for identifying stimuli that could be used to mobilize the latent reservoir for elimination. ..
  10. Latent Viral Reservoirs in HIV 1 Infection
    Robert Siliciano; Fiscal Year: 2009
    ..Curing HIV infection will require new ways to eliminate this latent reservoir. The studies proposed here are designed to find new drugs that can eliminate this latent reservoir. ..