POLD1

Summary

Gene Symbol: POLD1
Description: DNA polymerase delta 1, catalytic subunit
Alias: CDC2, CRCS10, MDPL, POLD, DNA polymerase delta catalytic subunit, CDC2 homolog, DNA polymerase subunit delta p125, polymerase (DNA directed), delta 1, catalytic subunit 125kDa, polymerase (DNA) delta 1, catalytic subunit
Species: human
Products:     POLD1

Top Publications

  1. SONG N, Zhu X, Shi L, An J, Wu Y, Sang J. Identification and functional analysis of a CDE/CHR element in the POLD1 promoter. Sci China C Life Sci. 2009;52:551-9 pubmed publisher
    DNA polymerase delta is encoded by the POLD1 gene, the transcription of which is strictly cell cycle-dependent. However, the means by which POLD1 transcription is regulated by the cell cycle mechanism is currently unknown...
  2. Wang J, Guo H, Wang P, Liu C. Age-dependent down-regulation of DNA polymerase ?1 in human lymphocytes. Mol Cell Biochem. 2012;371:157-63 pubmed publisher
    ..Our findings collectively confirmed that the down-regulations of DNA pol ?1 are age-related and have little bearing on diseases and nutritures. DNA pol ?1 has great potential for a new biomarker of aging. ..
  3. Naryzhny S, Lee H. The post-translational modifications of proliferating cell nuclear antigen: acetylation, not phosphorylation, plays an important role in the regulation of its function. J Biol Chem. 2004;279:20194-9 pubmed
    ..Taken together, our data support a model where the acidic and M forms participate in DNA replication, whereas the basic form is associated with the termination of DNA replication. ..
  4. Palles C, Cazier J, Howarth K, Domingo E, Jones A, Broderick P, et al. Germline mutations affecting the proofreading domains of POLE and POLD1 predispose to colorectal adenomas and carcinomas. Nat Genet. 2013;45:136-44 pubmed publisher
    ..sequencing, supplemented by linkage and association analysis, we identified specific heterozygous POLE or POLD1 germline variants in several multiple-adenoma and/or CRC cases but in no controls...
  5. Greer Y, Gao B, Yang Y, Nussenzweig A, Rubin J. Lack of Casein Kinase 1 Delta Promotes Genomic Instability - The Accumulation of DNA Damage and Down-Regulation of Checkpoint Kinase 1. PLoS ONE. 2017;12:e0170903 pubmed publisher
    ..kinase 1 (Chk1), which functions in the DNA damage response and mitotic checkpoints, and its downstream effector, Cdc2/CDK1 kinase, were often decreased in MEFCsnk1d null cells as well as in control MEFs transfected with CK1? siRNA...
  6. Chang M, Lin L, Wu M, Chan C, Chang H, Lee M, et al. Effects of Camphorquinone on Cytotoxicity, Cell Cycle Regulation and Prostaglandin E2 Production of Dental Pulp Cells: Role of ROS, ATM/Chk2, MEK/ERK and Hemeoxygenase-1. PLoS ONE. 2015;10:e0143663 pubmed publisher
    ..CQ also induced G2/M cell cycle arrest and apoptosis of pulp cells. The expression of type I collagen, cdc2, cyclin B, and cdc25C was inhibited, while p21, HO-1 and cyclooxygenase-2 (COX-2) were stimulated by CQ...
  7. Paronetto M, Zalfa F, Botti F, Geremia R, Bagni C, Sette C. The nuclear RNA-binding protein Sam68 translocates to the cytoplasm and associates with the polysomes in mouse spermatocytes. Mol Biol Cell. 2006;17:14-24 pubmed
    ..by inhibitors of the mitogen activated protein kinases ERK1/2 and of the maturation promoting factor cyclinB-cdc2 complex...
  8. Prichard M. Function of human cytomegalovirus UL97 kinase in viral infection and its inhibition by maribavir. Rev Med Virol. 2009;19:215-29 pubmed publisher
    ..Each of these is also phosphorylated and regulated by cdc2/cyclin-dependent kinase 1, suggesting that the viral kinase may perform a similar function...
  9. Wu L, Dong H, Zhao J, Wang Y, Yang Q, Jia C, et al. Diosgenin stimulates rat TM4 cell proliferation through activating plasma membrane translocation and transcriptional activity of estrogen receptors. Biol Reprod. 2015;92:24 pubmed publisher
    ..as well as CDK4/6 and CDK2 expression but inhibited P27 expression, with no significant alterations of cyclin B and cdc2 (cell division cycle 2), resulting in cell-cycle G1/S transition...

More Information

Publications141 found, 100 shown here

  1. Guo T, Song H, Zhao Z, Qi Z, Zhao S. Overexpression of Annexin A2 Receptor Inhibits Neovascularization via the Promotion of Krüppel-Like Transcription Factor 2. Cell Physiol Biochem. 2018;46:1617-1627 pubmed publisher
    ..A series of cell cycle-related proteins including cyclin A1, cyclin B1, cyclin D1, cyclin E1, CDK1, and p-CDC2 were detected by WB...
  2. Pan S, Qin Y, Zhou Z, He Z, Zhang X, Yang T, et al. Plumbagin induces G2/M arrest, apoptosis, and autophagy via p38 MAPK- and PI3K/Akt/mTOR-mediated pathways in human tongue squamous cell carcinoma cells. Drug Des Devel Ther. 2015;9:1601-26 pubmed publisher
    ..and time-dependent manner with a decrease in the expression level of cell division cycle protein 2 homolog (Cdc2) and cyclin B1 and increase in the expression level of p21 Waf1/Cip1, p27 Kip1, and p53 in SCC25 cells...
  3. Hass H, Vogel U, Scheurlen M, Jobst J. Gene-expression Analysis Identifies Specific Patterns of Dysregulated Molecular Pathways and Genetic Subgroups of Human Hepatocellular Carcinoma. Anticancer Res. 2016;36:5087-5095 pubmed
    ..g. cyclin B1, karyopherin alpha 2 (KPNA2), cyclin-dependent kinase 2 (CDC2)], G-protein depending signaling [e.g...
  4. Ahn S, Chang I, Kim K, Kim C, Namgung U, Cho C. Bogijetong decoction and its active herbal components protect the peripheral nerve from damage caused by taxol or nerve crush. BMC Complement Altern Med. 2016;16:402 pubmed
    ..Vimentin phosphorylation catalyzed by cell division cycle 2 (Cdc2) kinase was induced from Schwann cells in the sciatic nerves after taxol injection and crush injury, and phospho-..
  5. Qin C, Ren L, Ji M, Lv S, Wei Y, Zhu D, et al. CDKL1 promotes tumor proliferation and invasion in colorectal cancer. Onco Targets Ther. 2017;10:1613-1624 pubmed publisher
    CDKL1 is a member of the cell division cycle 2 (CDC2)-related serine threonine protein kinase family and is overexpressed in malignant tumors such as melanoma, breast cancer, and gastric cancer...
  6. Lu Y, You M, Ghazoui Z, Liu P, VEDELL P, Wen W, et al. Concordant effects of aromatase inhibitors on gene expression in ER+ Rat and human mammary cancers and modulation of the proteins coded by these genes. Cancer Prev Res (Phila). 2013;6:1151-61 pubmed publisher
    ..anaphase-prometaphase complex (APC) pathway, including Aurora-A kinase, BUBR1B, TOP2, cyclin A, cyclin B CDC2, and TPX-2)] were downregulated in animal and human studies reflecting the strong antiproliferative effects of ..
  7. Ma H, Jin S, Ju W, Fu Y, Tu Y, Wang L, et al. Stathmin is overexpressed and regulated by mutant p53 in oral squamous cell carcinoma. J Exp Clin Cancer Res. 2017;36:109 pubmed publisher
    ..Poly ADP ribose polymerase (PARP) and cyclin-dependent kinase 1 (cdc2) were activated after silencing the expression of stathmin...
  8. Li X, Liang Q, Liu W, Zhang N, Xu L, Zhang X, et al. Ras association domain family member 10 suppresses gastric cancer growth by cooperating with GSTP1 to regulate JNK/c-Jun/AP-1 pathway. Oncogene. 2016;35:2453-64 pubmed publisher
    ..These were confirmed by decreased expression of proliferation markers (proliferating cell nuclear antigen, p-CDC2 and p-CDC25) and increased apoptotic cascades (cleaved caspases-9, -8, -3 and cleaved poly (ADP-ribose) polymerase)...
  9. Shen Y, Liu Q, Lou S, Luo Y, Sun H, Zeng H, et al. Decreased expression of the augmenter of liver regeneration results in growth inhibition and increased chemosensitivity of acute T lymphoblastic leukemia cells. Oncol Rep. 2017;38:3130-3136 pubmed publisher
    ..siRNA/ALR in combination with VCR resulted in prolonged G2/M arrest, accompanied by downregulation of cdc25c and cdc2 expression and dissociation of cyclin B1...
  10. Shieh P, Jan C, Liang W. The protective effects of the antioxidant N-acetylcysteine (NAC) against oxidative stress-associated apoptosis evoked by the organophosphorus insecticide malathion in normal human astrocytes. Toxicology. 2019;417:1-14 pubmed publisher
    ..Cell cycle-related protein levels (cyclin E1, CDK2, cyclin A2, CDK1/CDC2, or cyclin B1) and apoptotic protein levels (Bcl-2, Bax, and cleaved caspase-9/caspase-3) were analyzed by Western ..
  11. Li J, Yang Y, Liu Q, Pan S, He Z, Zhang X, et al. The investigational Aurora kinase A inhibitor alisertib (MLN8237) induces cell cycle G2/M arrest, apoptosis, and autophagy via p38 MAPK and Akt/mTOR signaling pathways in human breast cancer cells. Drug Des Devel Ther. 2015;9:1627-52 pubmed publisher
    ..of p21 Waf1/Cip1, p27 Kip1, and p53, suggesting that ALS induces G2/M arrest through modulation of p53/p21/CDC2/cyclin B1 pathways...
  12. Naert G, Ferré V, Meunier J, Keller E, Malmström S, Givalois L, et al. Leucettine L41, a DYRK1A-preferential DYRKs/CLKs inhibitor, prevents memory impairments and neurotoxicity induced by oligomeric Aβ25-35 peptide administration in mice. Eur Neuropsychopharmacol. 2015;25:2170-82 pubmed publisher
    Dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs) and cdc2-like kinases (CLKs) are implicated in the onset and progression of Down syndrome (DS) and Alzheimer's disease (AD)...
  13. Jung C, Hong J, Bae S, Kang S, Park H, Lee S. Antitumor Activity of Americanin A Isolated from the Seeds of Phytolacca americana by Regulating the ATM/ATR Signaling Pathway and the Skp2-p27 Axis in Human Colon Cancer Cells. J Nat Prod. 2015;78:2983-93 pubmed publisher
    ..Therefore, compound 1 suppressed the activity of cyclin B1 and its partner cell division cycle 2 (cdc2) to prevent entry into mitosis...
  14. Chang M, Tsai Y, Liou E, Tang C, Wang T, Liu H, et al. Effect of Butyrate on Collagen Expression, Cell Viability, Cell Cycle Progression and Related Proteins Expression of MG-63 Osteoblastic Cells. PLoS ONE. 2016;11:e0165438 pubmed publisher
    ..Some cell apoptosis was noted. The mRNA expression of cdc2 and cyclin-B1 decreased after exposure to butyrate...
  15. Fettucciari K, Ponsini P, Gioè D, Macchioni L, Palumbo C, Antonelli E, et al. Enteric glial cells are susceptible to Clostridium difficile toxin B. Cell Mol Life Sci. 2017;74:1527-1551 pubmed publisher
    ..following in EGCs: (1) early cell rounding with Rac1 glucosylation; (2) early G2/M cell-cycle arrest by cyclin B1/Cdc2 complex inactivation caused by p27 upregulation, the downregulation of cyclin B1 and Cdc2 phosphorylated at Thr161 ..
  16. Pereira S, Monteiro M, Bourdeau I, Lacroix A, Pignatelli D. MECHANISMS OF ENDOCRINOLOGY: Cell cycle regulation in adrenocortical carcinoma. Eur J Endocrinol. 2018;179:R95-R110 pubmed publisher
    ..We have analyzed the most extensively studied checkpoints: the p53/Rb1 pathway, CDC2/cyclin B and topoisomerases (TOPs)...
  17. Liu S, Liu B. Overexpression of Nitrogen Permease Regulator Like-2 (NPRL2) Enhances Sensitivity to Irinotecan (CPT-11) in Colon Cancer Cells by Activating the DNA Damage Checkpoint Pathway. Med Sci Monit. 2018;24:1424-1433 pubmed
    ..proteins (MMP2, MMP9, p-PI3K, and p-AKT), and DNA damage checkpoint pathway proteins (p-ATM, p-Chk2, Cdc25C, Cdc2, and Cyclin B1) were quantified by Western blotting...
  18. Shetake N, Kumar A, Pandey B. Iron-oxide nanoparticles target intracellular HSP90 to induce tumor radio-sensitization. Biochim Biophys Acta Gen Subj. 2019;1863:857-869 pubmed publisher
    ..with HSP90, resulting in down-regulation of its client proteins, involved in cell cycle progression (Cyclin B1 and CDC2) and DNA-double strand break repair (e.g. RAD51 and BRCA1)...
  19. Li L, Chen X, Liu C, Lee L, Man C, Cheng S. Phytoestrogen Bakuchiol Exhibits In Vitro and In Vivo Anti-breast Cancer Effects by Inducing S Phase Arrest and Apoptosis. Front Pharmacol. 2016;7:128 pubmed publisher
    ..The S phase arrest was accompanied by the upregulation of ATM, P-Cdc2 (Tyr15), Myt1, P-Wee1 (Ser642), p21 and Cyclin B1, suggesting that blocking of Cdc2 activation may play an ..
  20. Fettucciari K, Macchioni L, Davidescu M, Scarpelli P, Palumbo C, Corazzi L, et al. Clostridium difficile toxin B induces senescence in enteric glial cells: A potential new mechanism of Clostridium difficile pathogenesis. Biochim Biophys Acta Mol Cell Res. 2018;1865:1945-1958 pubmed publisher
    ..DNA damage (ATM and H2AX phosphorylation), p27 overexpression, pRB hypophosphorylation, c‑Myc, cyclin B1, cdc2 and phosphorylated-cdc2 downregulation, Sirtuin‑2 and Sirtuin‑3 overexpression...
  21. Hu P, Zuo Z, Wang F, Peng X, Guan K, Li H, et al. The Protective Role of Selenium in AFB1-Induced Tissue Damage and Cell Cycle Arrest in Chicken's Bursa of Fabricius. Biol Trace Elem Res. 2018;185:486-496 pubmed publisher
    ..ATM, Chk2, p53, p21, cdc25, PCNA, cyclin D1, cyclin E1, cyclin B3, CDK6, CDK2, and cdc2 indicated that Se supplement could restore these parameters to be close to those in the control group...
  22. Megiorni F, McDowell H, Camero S, Mannarino O, Ceccarelli S, Paiano M, et al. Crizotinib-induced antitumour activity in human alveolar rhabdomyosarcoma cells is not solely dependent on ALK and MET inhibition. J Exp Clin Cancer Res. 2015;34:112 pubmed publisher
    ..G2/M phase was attributed to an altered expression and activation of checkpoint regulators, such as Cyclin B1 and Cdc2. Crizotinib was able to induce apoptosis and autophagy in a dose-dependent manner, as shown by caspase-3 activation/..
  23. Zhao J, Liao Y, Chen J, Dong X, Gao Z, Zhang H, et al. Aberrant Buildup of All-Trans-Retinal Dimer, a Nonpyridinium Bisretinoid Lipofuscin Fluorophore, Contributes to the Degeneration of the Retinal Pigment Epithelium. Invest Ophthalmol Vis Sci. 2017;58:1063-1075 pubmed publisher
    ..also corroborated by a remarkable abnormality in expression of proteins associated with cell cycle (Cyclin B1 and Cdc2) and apoptosis (p53, Bcl-2 and Bax) in the RPE of Abca4-/-Rdh8-/- DKO mice...
  24. Sozmen M, Devrim A, Kabak Y, Devrim T, Sudagidan M. The Effects of Periostin in a Rat Model of Isoproterenol: Mediated Cardiotoxicity. Cardiovasc Toxicol. 2018;18:142-160 pubmed publisher
    ..apoptosis (Bcl-2, Bax, PCNA, Ki-67, phospho-histone H3), cell cycle activators and inhibitors (cyclin D1, D2, A2, Cdc2), the origin of regenerating cells (cKit and CD45) mRNA were detected using quantitative real-time polymerase chain ..
  25. Klopsch C, Skorska A, Ludwig M, Lemcke H, Maass G, Gaebel R, et al. Intramyocardial angiogenetic stem cells and epicardial erythropoietin save the acute ischemic heart. Dis Model Mech. 2018;11: pubmed publisher
    ..EPO treatment enhanced levels of intracardiac regenerative key indicators (SDF-1, CXCR4, CD34, Bcl-2, cyclin D1, Cdc2 and MMP2), induced transforming growth factor β (TGF-β)/WNT signaling in intramyocardial MSC niches through the ..
  26. Zeng Q, Yi H, Huang L, An Q, Wang H. Long-term arsenite exposure induces testicular toxicity by redox imbalance, G2/M cell arrest and apoptosis in mice. Toxicology. 2019;411:122-132 pubmed publisher
    ..could induce G2/M phase arrest in testes, concurrent with a significant decrease in mRNA and protein levels of cdc2 and cyclin B1, the upregulation of p-cdc2, and an increase in mRNA levels of p53 and p21...
  27. Chang I, Lim H, Kim K, Shin H, Namgung U. Enhanced axonal regeneration of the injured sciatic nerve by administration of Buyang Huanwu decoction. J Ethnopharmacol. 2016;194:626-634 pubmed publisher
    ..In vivo administration of BYHWD in rats upregulated the induction level of cell division cycle 2 (Cdc2) and its phosphorylation of vimentin in Schwann cells from injured sciatic nerve...
  28. Klumpp D, Frank S, Klumpp L, Sezgin E, Eckert M, Edalat L, et al. TRPM8 is required for survival and radioresistance of glioblastoma cells. Oncotarget. 2017;8:95896-95913 pubmed publisher
    ..TRPM8-mediated Ca2+ signaling that interfered with cell cycle control probably via CaMKII, cdc25C and cdc2. Combined, our data suggest that TRPM8 channels contribute to spreading, survival and radioresistance of human ..
  29. Mao Y. Analysis of Mitotic Checkpoint Function in Xenopus Egg Extracts. Cold Spring Harb Protoc. 2018;2018:pdb.prot099853 pubmed publisher
    ..Simple DAPI staining for chromatin structure or biochemical analysis of Cdc2/cyclin B (cyclin-dependent kinase) histone H1 kinase activity can be used to evaluate the stages of the cell cycle ..
  30. Luo A, Zhang X, Fu L, Zhu Z, Dong J. Zinc finger factor ZNF121 is a MYC-interacting protein functionally affecting MYC and cell proliferation in epithelial cells. J Genet Genomics. 2016;43:677-685 pubmed publisher
    ..g., EGR1, CDC2, and nucleolin) and slowed cell proliferation, accompanied by cell cycle arrest in the G1 phase and expression ..
  31. Vaara M, Itkonen H, Hillukkala T, Liu Z, Nasheuer H, Schaarschmidt D, et al. Segregation of replicative DNA polymerases during S phase: DNA polymerase ?, but not DNA polymerases ?/?, are associated with lamins throughout S phase in human cells. J Biol Chem. 2012;287:33327-38 pubmed
  32. Leone D, Kozakowski N, Kornauth C, Waidacher T, Neudert B, Loeffler A, et al. The Phenotypic Characterization of the Human Renal Mononuclear Phagocytes Reveal a Co-Ordinated Response to Injury. PLoS ONE. 2016;11:e0151674 pubmed publisher
    ..3%) when pDC were sparse and sparse (12.8-12.5%) when pDC were abundant, whereas the proportions of cDC2 cells and classical monocytes increased slightly in pDC high kidneys...
  33. Wang H, Zhang Y, Zhou Y, Lu Y, Wang W, Xin M, et al. Rosiglitazone elevates sensitization of drug-resistant oral epidermoid carcinoma cells to vincristine by G2/M-phase arrest, independent of PPAR-? pathway. Biomed Pharmacother. 2016;83:349-361 pubmed publisher
    ..The effect of G2/M phase arrest was associated with the upregulation of cyclin B1 and downregulation of p-cdc2. The apoptosis induction of ROSI and VCR was partly due to an upregulation of cleaved PARP and downregulation of ..
  34. Wang H, Gao X, Lu X, Wang Y, Ma C, Shi Z, et al. The mitotic regulator Hec1 is a critical modulator of prostate cancer through the long non-coding RNA BX647187 in vitro. Biosci Rep. 2015;35: pubmed publisher
    ..Cells viability was evaluated using cell counting Kit-8. Cyclin B1-Cdc2 (cell division cycle 2) activity was measured by ELISA assay...
  35. Yue J, López J. JNK does not regulate meiotic progression in Xenopus oocytes: The strange case of pJNK and pERK. Dev Biol. 2016;416:42-51 pubmed publisher
    ..Cdc25C and inhibition of the protein kinase Myt1, thus promoting dephosphorylation and activation of cyclinB/Cdc2 (MPF)...
  36. Castrec B, Laurent S, Henneke G, Flament D, Raffin J. The glycine-rich motif of Pyrococcus abyssi DNA polymerase D is critical for protein stability. J Mol Biol. 2010;396:840-8 pubmed publisher
    ..These studies suggest that the (G)-PYF box motif mediates intersubunit interactions and that it may be crucial for the thermostability of PabPol D...
  37. Ghodgaonkar M, Kehl P, Ventura I, Hu L, Bignami M, Jiricny J. Phenotypic characterization of missense polymerase-δ mutations using an inducible protein-replacement system. Nat Commun. 2014;5:4990 pubmed publisher
    ..This proof-of-principle study demonstrates the general applicability of this experimental approach in the study of genotype-phenotype correlations. ..
  38. Liang D, Li J, Wu Y, Zhen L, Li C, Qi M, et al. miRNA-204 drives cardiomyocyte proliferation via targeting Jarid2. Int J Cardiol. 2015;201:38-48 pubmed publisher
    ..Accordingly, the cell cycle regulators, including Cyclin A, Cyclin B, Cyclin D2, Cyclin E, CDC2 and PCNA, were upregulated in miRNA-204 transgenic embryonic hearts...
  39. Sun F, Huang L. A euryarchaeal histone modulates strand displacement synthesis by replicative DNA polymerases. Sci China Life Sci. 2016;59:709-16 pubmed publisher
    ..Euryarchaea have two distinct replicative DNA polymerases, PolB and PolD, a family D DNA polymerase...
  40. Feng L, Yang X, Asweto C, Wu J, Zhang Y, Hu H, et al. Low-dose combined exposure of nanoparticles and heavy metal compared with PM2.5 in human myocardial AC16 cells. Environ Sci Pollut Res Int. 2017;24:27767-27777 pubmed publisher
    ..of protein Bcl-2; and trigger G2/M phase arrest by the upregulation of protein Chk2 and downregulation of protein Cdc2 and cyclin B1...
  41. Riggs J, Nagy M, Elsner J, Erdman P, Cashion D, Robinson D, et al. The Discovery of a Dual TTK Protein Kinase/CDC2-Like Kinase (CLK2) Inhibitor for the Treatment of Triple Negative Breast Cancer Initiated from a Phenotypic Screen. J Med Chem. 2017;60:8989-9002 pubmed publisher
    ..These data supported the nomination of CC-671 into IND-enabling studies as a single agent TNBC therapy...
  42. Yang C, Chang L, Zhang P, Hao H, Zhu L, Toomey N, et al. Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta. Nucleic Acids Res. 1992;20:735-45 pubmed
    ..The human pol delta gene was localized to chromosome 19 by Southern blotting of EcoRI digested DNA from a panel of rodent/human cell hybrids. ..
  43. Mohammadi A, Mansoori B, Aghapour M, Shirjang S, Nami S, Baradaran B. The Urtica dioica extract enhances sensitivity of paclitaxel drug to MDA-MB-468 breast cancer cells. Biomed Pharmacother. 2016;83:835-842 pubmed publisher
    ..The expression levels of snail-1, ZEB1, ZEB2, twist, Cdc2, cyclin B1 and Wee1 genes were quantified using qRT-PCR and western blot performed for snail-1expression...
  44. Yin X, Yu H, Jin X, Li J, Guo H, Shi Q, et al. Human Blood CD1c+ Dendritic Cells Encompass CD5high and CD5low Subsets That Differ Significantly in Phenotype, Gene Expression, and Functions. J Immunol. 2017;198:1553-1564 pubmed publisher
    ..Comparative transcriptome analyses showed that the CD5high DCs expressed higher levels of cDC2-specific genes, including IFN regulatory factor 4, which is essential for the cDC2 development and its migration to ..
  45. Chen X, Liao Y, Long D, Yu T, Shen F, Lin X. The Cdc2/Cdk1 inhibitor, purvalanol A, enhances the cytotoxic effects of taxol through Op18/stathmin in non-small cell lung cancer cells in vitro. Int J Mol Med. 2017;40:235-242 pubmed publisher
    Purvalanol A is a highly selective inhibitor of Cdc2 [also known as cyclin-dependent kinase 1 (CDK1)]. Taxol is an anti-tumor chemotherapeutic drug which is widely used clinically...
  46. Anderson D, Murphy K, Briseño C. Development, Diversity, and Function of Dendritic Cells in Mouse and Human. Cold Spring Harb Perspect Biol. 2017;: pubmed publisher
    ..Models of transcription factor deficiency have been used to identify subsets of cDC2 that are required for T helper (Th)2 and Th17 responses to certain pathogens; however, no single factor is known to ..
  47. Dong M, Rahman M, Tarbell K. Flow cytometric gating for spleen monocyte and DC subsets: differences in autoimmune NOD mice and with acute inflammation. J Immunol Methods. 2016;432:4-12 pubmed publisher
    ..A simple but informative nomenclature of conventional and plasmacytoid dendritic cell subsets (cDC1, cDC2, pDC) and monocyte-derived populations incorporates these advances, but accurate subset identification is critical...
  48. Wang X, Sun C, Hageman L, Smith K, Singh P, Desai S, et al. Clinical and Genetic Risk Prediction of Subsequent CNS Tumors in Survivors of Childhood Cancer: A Report From the COG ALTE03N1 Study. J Clin Oncol. 2017;35:3688-3696 pubmed publisher
    ..nucleotide polymorphisms (rs15869 [ BRCA2], rs1805389 [ LIG4], rs8079544 [ TP53], rs25489 [ XRCC1], rs1673041 [ POLD1], and rs11615 [ ERCC1]) and subsequent CNS tumors in survivors of childhood cancer...
  49. Chan N, Guo J, Zhang T, Mao G, Hou C, Yuan F, et al. Coordinated processing of 3' slipped (CAG)n/(CTG)n hairpins by DNA polymerases ? and ? preferentially induces repeat expansions. J Biol Chem. 2013;288:15015-22 pubmed publisher
    ..These findings strongly suggest that coordinated processing of 3'-slipped (CAG)n/(CTG)n hairpins by polymerases ? and ? on during DNA synthesis induces CAG/CTG repeat expansions. ..
  50. Li Y, Li S, Ho C, Chang Y, Tan K, Chung T, et al. Tangeretin derivative, 5-acetyloxy-6,7,8,4'-tetramethoxyflavone induces G2/M arrest, apoptosis and autophagy in human non-small cell lung cancer cells in vitro and in vivo. Cancer Biol Ther. 2016;17:48-64 pubmed publisher
    ..that 5-AcTMF effectively inhibited cancer cell proliferation, induced G2/M-phase arrest associated with cdc2 and CDC25c and increased in the apoptotic cells associated with caspase activation, down regulation of Bcl-2, XIAP ..
  51. Mondal A, Guria T, Maity T, Bishayee A. A Novel Tetraenoic Fatty Acid Isolated from Amaranthus spinosus Inhibits Proliferation and Induces Apoptosis of Human Liver Cancer Cells. Int J Mol Sci. 2016;17: pubmed publisher
    ..viability, flow cytometry assay for cell cycle analysis, and Western blot analysis to measure protein expression of Cdc2), cyclin B1, Bcl-2-associated X protein (Bax), and B-cell lymphoma 2 (Bcl-2)...
  52. Chien M, Lee T, Lee W, Yeh Y, Li T, Wang P, et al. Trichodermin induces c-Jun N-terminal kinase-dependent apoptosis caused by mitotic arrest and DNA damage in human p53-mutated pancreatic cancer cells and xenografts. Cancer Lett. 2017;388:249-261 pubmed publisher
    ..Trichodermin also increased apoptosis through mitotic arrest by activating Cdc2/cyclin B1 complex activity...
  53. Dai Y, Xie H, Xu Y. Evaluation of deoxynivalenol-induced toxic effects on mouse endometrial stromal cells: Cell apoptosis and cell cycle. Biochem Biophys Res Commun. 2017;483:572-577 pubmed publisher
    ..After DON treatment, the expression levels of cell cycle-related protein including p38/p-p38, Cdc25C/p-Cdc25C, Cdc2/p-Cdc2 and cyclinB1 were significantly decreased and immunoprecipitation analysis showed that cyclinB1-Cdc2 complex ..
  54. Wang J, Zhang Z, Che Y, Yuan Z, Lü Z, Li Y, et al. Rabdocoestin B exhibits antitumor activity by inducing G2/M phase arrest and apoptosis in esophageal squamous cell carcinoma. Cancer Chemother Pharmacol. 2018;81:469-481 pubmed publisher
    ..arrest in ESCC cells by upregulating the Chk1/Chk2-Cdc25C axis to inhibit the G2→M transition facilitated by Cdc2/Cyclin B1...
  55. Karkhanis V, Wang L, Tae S, Hu Y, Imbalzano A, SIF S. Protein arginine methyltransferase 7 regulates cellular response to DNA damage by methylating promoter histones H2A and H4 of the polymerase ? catalytic subunit gene, POLD1. J Biol Chem. 2012;287:29801-14 pubmed publisher
    ..findings show that PRMT7 negatively regulates expression of genes involved in DNA repair, including ALKBH5, APEX2, POLD1, and POLD2...
  56. Yuan X, Sun X, Shi X, Jiang C, Yu D, Zhang W, et al. USP39 promotes the growth of human hepatocellular carcinoma in vitro and in vivo. Oncol Rep. 2015;34:823-32 pubmed publisher
    ..Moreover, USP39 knockdown led to the upregulation of p-Cdc2 and downregulation of p-Cdc25c and p-myt1, while the expression of total Cdc2, Cdc25c and myt1 was not changed in ..
  57. Wu J, Hong Y, Yang X. Bis(acetylacetonato)-oxidovanadium(IV) and sodium metavanadate inhibit cell proliferation via ROS-induced sustained MAPK/ERK activation but with elevated AKT activity in human pancreatic cancer AsPC-1 cells. J Biol Inorg Chem. 2016;21:919-929 pubmed
    ..In the presence of MEK-1 inhibitor, the degradation of Cdc25C, inactivation of Cdc2 and accumulation of p21 were relieved. However, the treatment of AKT inhibitor did not cause any significant effect...
  58. Esteban Jurado C, Giménez Zaragoza D, Muñoz J, Franch Expósito S, Álvarez Barona M, Ocaña T, et al. POLE and POLD1 screening in 155 patients with multiple polyps and early-onset colorectal cancer. Oncotarget. 2017;8:26732-26743 pubmed publisher
    Germline mutations in POLE and POLD1 have been shown to cause predisposition to colorectal multiple polyposis and a wide range of neoplasms, early-onset colorectal cancer being the most prevalent...
  59. Rothe C, Rødland G, Anda S, Stonyte V, Boye E, López Avilés S, et al. A checkpoint-independent mechanism delays entry into mitosis after UV irradiation. J Cell Sci. 2017;130:4028-4037 pubmed publisher
    ..The pathway delays activation of the mitotic cyclin-dependent kinase (CDK) Cdc2 after UV irradiation...
  60. Jin R, Chen Q, Yao S, Bai E, Fu W, Wang L, et al. Synthesis and anti-tumor activity of EF24 analogues as IKKβ inhibitors. Eur J Med Chem. 2018;144:218-228 pubmed publisher
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    ..in tumours is frequently associated with somatic mutations in the exonuclease domains of DNA polymerases POLE or POLD1, and results in a characteristic mutational profile...
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    ..at the G2/M transition leading to growth arrest and apoptosis; (b) by decreasing the expression of mitotic kinase cdc2, mitotic phosphatase cdc25c, mitotic cyclin B1, and apoptotic factors pro-caspases-3 and -9; and (c) increasing the ..
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    ..Patients with higher baseline expression of a set of specific pathway-related genes (BIRC5, AURKA, TTK, CDC2, and CCNB1) were more likely to respond in an exploratory biomarker analysis...
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    Nuclear gene markers, phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold), have been developed for precise discrimination of Fasciola flukes instead of internal transcribed spacer 1...
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    ..b>Cdc2-like kinase 1 (CLK1) in the host cells is responsible for alternative splicing of the M2 gene of influenza virus ..
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  79. Jansen A, van Wezel T, van den Akker B, Ventayol García M, Ruano D, Tops C, et al. Combined mismatch repair and POLE/POLD1 defects explain unresolved suspected Lynch syndrome cancers. Eur J Hum Genet. 2016;24:1089-92 pubmed publisher
    ..patients, we analyzed leukocyte and tumor DNA of 62 sLS patients using gene panel sequencing including the POLE, POLD1 and MMR genes. Forty tumors showed either one, two or more somatic MMR variants predicted to affect function...
  80. Hu M, Chen L, Chen Y, Tsai M, Shiau C, Chao T, et al. Targeting SHP-1-STAT3 signaling: A promising therapeutic approach for the treatment of cholangiocarcinoma. Oncotarget. 2017;8:65077-65089 pubmed publisher
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    ..COX-2 expression, ADAM9 maturation, and the ANE-induced decline in keratin 5 and 14, but showed little effect on cdc2 expression and EGF production...
  82. Mohammed H, Abd El Hafeez A, Abbas S, Abdelhafez E, Abuo Rahma G. New antiproliferative 7-(4-(N-substituted carbamoylmethyl)piperazin-1-yl) derivatives of ciprofloxacin induce cell cycle arrest at G2/M phase. Bioorg Med Chem. 2016;24:4636-4646 pubmed publisher
    ..Also, 2b increased the expression of p53 and p21 and decreased the expression of cyclin B1 and Cdc2 proteins in A549 cells without any effect on the same proteins expression in WI-38 cells...
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    ..tumors harbored more somatic mutations compared to LS cases, especially in the TP53 gene and in POLE and POLD1, where novel mutations were additionally identified...
  84. Zhang Y, Gong F, Lu Z, Wang H, Cheng Y, Liu Z, et al. DHPAC, a novel synthetic microtubule destabilizing agent, possess high anti-tumor activity in vincristine-resistant oral epidermoid carcinoma in vitro and in vivo. Int J Biochem Cell Biol. 2017;93:1-11 pubmed publisher
    ..g. Cyclin B1, Cdc2, Cdc25b, Cdc25c, RSK2). Furthermore, DHPAC presence inhibits PTEN phosphorylation and PTEN/Akt/NF-κB signalling...
  85. Okazaki H, Takeda S, Kakizoe K, Taniguchi A, Tokuyasu M, Himeno T, et al. Bisphenol AF as an Inducer of Estrogen Receptor ? (ER?): Evidence for Anti-estrogenic Effects at Higher Concentrations in Human Breast Cancer Cells. Biol Pharm Bull. 2017;40:1909-1916 pubmed publisher
    ..abrogation of estrogen response element (ERE)-mediated transcriptional activities as well as the down-regulation of Cdc2 expression in MCF-7 cells, without influencing the expression of ER?...
  86. Buchanan D, Stewart J, Clendenning M, Rosty C, Mahmood K, Pope B, et al. Risk of colorectal cancer for carriers of a germ-line mutation in POLE or POLD1. Genet Med. 2018;20:890-895 pubmed publisher
    Germ-line mutations in the exonuclease domains of the POLE and POLD1 genes are associated with an increased, but yet unquantified, risk of colorectal cancer (CRC)...
  87. Lu Y, Li W. Functional characterization of E2F3b in human HepG2 liver cancer cell line. J Cell Biochem. 2017;: pubmed publisher
    ..Six genes, including CDC2, CCNE1, ARF, MAP4K2, MUSK and PAX2 were confirmed to be upregulated by more than twofold; one gene, CCNA2 was ..
  88. Lessel D, Hisama F, Szakszon K, Saha B, Sanjuanelo A, Salbert B, et al. POLD1 Germline Mutations in Patients Initially Diagnosed with Werner Syndrome. Hum Mutat. 2015;36:1070-9 pubmed publisher
    ..Germline POLD1 mutations were recently reported in five patients with another segmental progeroid disorder: mandibular hypoplasia,..
  89. Altamura G, Corteggio A, Pacini L, Conte A, Pierantoni G, Tommasino M, et al. Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo. Virology. 2016;496:1-8 pubmed publisher
    ..pRb and impaired pRb levels, resulting in upregulation of the downstream pro-proliferative genes Cyclin A and Cdc2. Importantly, we demonstrated mRNA expression of FcaPV2 E2, E6 and E7 in feline SCC samples, strengthening the ..
  90. Czubaty A, Piekiełko Witkowska A. Protein kinases that phosphorylate splicing factors: Roles in cancer development, progression and possible therapeutic options. Int J Biochem Cell Biol. 2017;91:102-115 pubmed publisher
    ..g. SR-protein kinases, cdc2-like kinases, topoisomerase 1), and protein kinases that govern key cellular signaling pathways (i.e. AKT)...
  91. Cao L, Liu J, Pu J, Collinson J, Forrester J, McCaig C. Endogenous bioelectric currents promote differentiation of the mammalian lens. J Cell Physiol. 2018;233:2202-2212 pubmed publisher
    ..In addition, applied EF activated the AKT and CDC2 and inhibition of AKT reduced the activation of CDC2...
  92. Sun Q, Lin G, Li L, Jin X, Huang L, Zhang G, et al. Discovery of Potent and Selective Inhibitors of Cdc2-Like Kinase 1 (CLK1) as a New Class of Autophagy Inducers. J Med Chem. 2017;60:6337-6352 pubmed publisher
    ..However, safe autophagy inducers for clinical applications are lacking. Inhibition of cdc2-like kinase 1 (CLK1) was recently found to efficiently induce autophagy...