PLD1

Summary

Gene Symbol: PLD1
Description: phospholipase D1
Alias: CVDD, phospholipase D1, choline phosphatase 1, phosphatidylcholine-hydrolyzing phospholipase D1, phospholipase D1, phosphatidylcholine-specific
Species: human
Products:     PLD1

Top Publications

  1. Lee S, Park J, Kim Y, Kim J, Shin K, Lee J, et al. Actin directly interacts with phospholipase D, inhibiting its activity. J Biol Chem. 2001;276:28252-60 pubmed
    ..We also observed that beta-actin regulates PLD1 and PLD2 with similar binding and inhibitory potencies...
  2. Du G, Huang P, Liang B, Frohman M. Phospholipase D2 localizes to the plasma membrane and regulates angiotensin II receptor endocytosis. Mol Biol Cell. 2004;15:1024-30 pubmed
    ..Two PLD isoforms, PLD1 and PLD2, exist in mammals...
  3. Lee H, Park J, Jang I, Chae Y, Kim J, Kim I, et al. Munc-18-1 inhibits phospholipase D activity by direct interaction in an epidermal growth factor-reversible manner. J Biol Chem. 2004;279:16339-48 pubmed
    ..These results suggest that Munc-18-1 is a potent negative regulator of basal PLD activity and that EGF stimulation abolishes this interaction. ..
  4. Kim J, Han J, Lee S, Kim Y, Lee T, Park J, et al. Phospholipase D1 in caveolae: regulation by protein kinase Calpha and caveolin-1. Biochemistry. 1999;38:3763-9 pubmed
    ..Previously we have demonstrated that protein kinase Calpha (PKCalpha) directly interacts with phospholipase D1 (PLD1), activating the enzymatic activity of PLD1 in the presence of phorbol 12-myristate 13-acetate (PMA) [..
  5. Hitomi T, Zhang J, Nicoletti L, Grodzki A, Jamur M, Oliver C, et al. Phospholipase D1 regulates high-affinity IgE receptor-induced mast cell degranulation. Blood. 2004;104:4122-8 pubmed
    ..the role of phospholipase D (PLD) in FcepsilonRI signaling, the wild-type or the catalytically inactive forms of PLD1 or PLD2 were stably overexpressed in RBL-2H3 mast cells...
  6. Han J, Kim Y, Lee J, Lee C, Lee B, Ohba M, et al. Localization of phospholipase D1 to caveolin-enriched membrane via palmitoylation: implications for epidermal growth factor signaling. Mol Biol Cell. 2002;13:3976-88 pubmed
    ..We investigated the importance of the phosphorylation and compartmentalization of PLD1 in EGF signaling...
  7. Han L, Stope M, De Jesús M, Oude Weernink P, Urban M, Wieland T, et al. Direct stimulation of receptor-controlled phospholipase D1 by phospho-cofilin. EMBO J. 2007;26:4189-202 pubmed
    ..We demonstrate that muscarinic receptor-mediated stimulation of phospholipase D1 (PLD1) is controlled by LIM-kinase, slingshot phosphatase as well as 14-3-3zeta, and requires ..
  8. Jang Y, Namkoong S, Kim Y, Lee S, Park B, Min D. Cleavage of phospholipase D1 by caspase promotes apoptosis via modulation of the p53-dependent cell death pathway. Cell Death Differ. 2008;15:1782-93 pubmed publisher
    ..However, the mechanisms regulating PLD activity during apoptosis remain unknown. Here we report that cleavage of PLD1 by caspases facilitates p53-mediated apoptosis...
  9. Ahn B, Kim S, Kim E, Choi K, Kwon T, Lee Y, et al. Transmodulation between phospholipase D and c-Src enhances cell proliferation. Mol Cell Biol. 2003;23:3103-15 pubmed
    ..We demonstrate for the first time that most notably PLD2 and to a lesser extent the PLD1 isoform are tyrosine phosphorylated by c-Src tyrosine kinase via direct association...

More Information

Publications90

  1. Gao S, Murakami M, Ito H, Furuhata A, Yoshida K, Tagawa Y, et al. Mutated ras induced PLD1 gene expression through increased Sp1 transcription factor. Nagoya J Med Sci. 2009;71:127-36 pubmed
    ..Ras-mutation and activation were correlated, and cells with enhanced ras-activation showed increased PLD1 mRNA and protein...
  2. Jang Y, Min D. Nuclear localization of phospholipase D1 mediates the activation of nuclear protein kinase C(alpha) and extracellular signal-regulated kinase signaling pathways. J Biol Chem. 2011;286:4680-9 pubmed publisher
    ..In the present study we have identified the nuclear localization sequence (NLS) of PLD1 whose mutation abolished its nuclear import...
  3. Chen Q, Hongu T, Sato T, Zhang Y, Ali W, Cavallo J, et al. Key roles for the lipid signaling enzyme phospholipase d1 in the tumor microenvironment during tumor angiogenesis and metastasis. Sci Signal. 2012;5:ra79 pubmed publisher
    ..We report that ablation of the lipid signaling enzyme phospholipase D1 (PLD1) in the tumor environment compromised the neovascularization and growth of tumors...
  4. Gomez Cambronero J. Phosphatidic acid, phospholipase D and tumorigenesis. Adv Biol Regul. 2014;54:197-206 pubmed publisher
    ..Elevation of either PLD1 or PLD2 (the two mammalian isoforms of PLD) is able to transform fibroblasts and contribute to cancer progression...
  5. Chen Y, Zheng Y, Foster D. Phospholipase D confers rapamycin resistance in human breast cancer cells. Oncogene. 2003;22:3937-42 pubmed
    ..These data implicate mTOR as a critical target for survival signals generated by PLD and suggest that PLD levels in breast cancer could be a valuable indicator of the likely efficacy of rapamycin treatment. ..
  6. Kim J, Kim H, Jeon H, Suh P, Ryu S. Phospholipase D1 regulates cell migration in a lipase activity-independent manner. J Biol Chem. 2006;281:15747-56 pubmed
    ..by silencing endogenous PLD isozymes using small interfering RNA in HeLa cells, we demonstrate that endogenous PLD1 is required for the normal organization of the actin cytoskeleton, and, more importantly, for cell motility...
  7. Lee C, Kim I, Park J, Lee M, Lee H, Suh P, et al. The phox homology domain of phospholipase D activates dynamin GTPase activity and accelerates EGFR endocytosis. Nat Cell Biol. 2006;8:477-84 pubmed
    ..of PLDs was shown to retard EGFR endocytosis and the addition of wild-type PLDs or lipase-inactive PLDs, but not PLD1 mutants with defective GAP activity for dynamin in vitro, resulted in the recovery of EGFR endocytosis...
  8. Zhong M, Shen Y, Zheng Y, Joseph T, Jackson D, Foster D. Phospholipase D prevents apoptosis in v-Src-transformed rat fibroblasts and MDA-MB-231 breast cancer cells. Biochem Biophys Res Commun. 2003;302:615-9 pubmed
    ..We report here that elevated expression of either PLD1 or PLD2 in these cells prevents apoptosis induced by serum withdrawal...
  9. Riebeling C, Müller C, Geilen C. Expression and regulation of phospholipase D isoenzymes in human melanoma cells and primary melanocytes. Melanoma Res. 2003;13:555-62 pubmed
    ..As determined by reverse transcription polymerase chain reaction, both splicing variants of PLD1, PLD1a and PLD1b, and the isoenzyme PLD2, are expressed in melanoma cells and melanocytes...
  10. Sun Y, Fang Y, Yoon M, Zhang C, Roccio M, Zwartkruis F, et al. Phospholipase D1 is an effector of Rheb in the mTOR pathway. Proc Natl Acad Sci U S A. 2008;105:8286-91 pubmed publisher
    ..Here, we find that PLD1, but not PLD2, is required for Rheb activation of the mTOR pathway, as demonstrated by the effects of RNAi...
  11. Gozgit J, Pentecost B, Marconi S, Ricketts Loriaux R, Otis C, Arcaro K. PLD1 is overexpressed in an ER-negative MCF-7 cell line variant and a subset of phospho-Akt-negative breast carcinomas. Br J Cancer. 2007;97:809-17 pubmed
    ..and its parent cell line, MCF-7, identified 1402 differentially expressed transcripts, one of which was, phospholipase D1 (PLD1)...
  12. Jang Y, Ahn B, Namkoong S, Kim Y, Jin J, Kim Y, et al. Differential regulation of apoptosis by caspase-mediated cleavage of phospholipase D isozymes. Cell Signal. 2008;20:2198-207 pubmed publisher
    ..b>PLD1 is cleaved at one internal site (DDVD(545)S) and PLD2 is cleaved at two or three sites (PTGD(13)ELD(16)S and DEVD(..
  13. Brandenburg L, Seyferth S, Wruck C, Koch T, Rosenstiel P, Lucius R, et al. Involvement of Phospholipase D 1 and 2 in the subcellular localization and activity of formyl-peptide-receptors in the human colonic cell line HT29. Mol Membr Biol. 2009;26:371-83 pubmed publisher
    ..PLD is a key enzyme involved in secretion, endocytosis and receptor signalling. We inhibited PLD1 and 2 by small interference RNA (siRNA) and determined the activity of formyl peptide receptors using Western ..
  14. Kang D, Min D. Positive feedback regulation between phospholipase D and Wnt signaling promotes Wnt-driven anchorage-independent growth of colorectal cancer cells. PLoS ONE. 2010;5:e12109 pubmed publisher
    ..Here, we demonstrate that PLD isozymes, PLD1 and PLD2 are direct targets and positive feedback regulators of the Wnt/beta-catenin signaling...
  15. Park S, Chun Y, Ryu S, Suh P, Kim H. Assignment of human PLD1 to human chromosome band 3q26 by fluorescence in situ hybridization. Cytogenet Cell Genet. 1998;82:224 pubmed
  16. Colley W, Sung T, Roll R, Jenco J, Hammond S, Altshuller Y, et al. Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization. Curr Biol. 1997;7:191-201 pubmed
    ..We recently reported the cloning of the human gene encoding PLD1; this enzyme has low basal activity and is activated by protein kinase C and the small GTP-binding proteins, ADP-..
  17. Hammond S, Altshuller Y, Sung T, Rudge S, Rose K, Engebrecht J, et al. Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family. J Biol Chem. 1995;270:29640-3 pubmed
    ..Characterization of recombinant human PLD1 reveals that it is membrane-associated, selective for phosphatidylcholine, stimulated by phosphatidylinositol 4,5-..
  18. Hammond S, Jenco J, Nakashima S, Cadwallader K, Gu Q, Cook S, et al. Characterization of two alternately spliced forms of phospholipase D1. Activation of the purified enzymes by phosphatidylinositol 4,5-bisphosphate, ADP-ribosylation factor, and Rho family monomeric GTP-binding proteins and protein kinase C-alpha. J Biol Chem. 1997;272:3860-8 pubmed
    We previously reported the cloning of a cDNA encoding human phosphatidylcholine-specific phospholipase D1 (PLD1), an ADP-ribosylation factor (ARF)-activated phosphatidylcholine-specific phospholipase D (Hammond, S. M., Tsung, S...
  19. Iyer S, Agrawal R, Thompson C, Thompson S, Barton J, Kusner D. Phospholipase D1 regulates phagocyte adhesion. J Immunol. 2006;176:3686-96 pubmed
    ..Stimulation of PLD activity was rapid and persisted for at least 90 min. Confocal microscopy indicated that PLD1 exhibited partial colocalization with actin filaments at the adherent interface, in proximity to the focal adhesion ..
  20. Hui L, Rodrik V, Pielak R, Knirr S, Zheng Y, Foster D. mTOR-dependent suppression of protein phosphatase 2A is critical for phospholipase D survival signals in human breast cancer cells. J Biol Chem. 2005;280:35829-35 pubmed
    ..The ability of phospholipase D to suppress protein phosphatase 2A identifies a critical target of an emerging phospholipase D/mTOR survival pathway in the transformation of human cells. ..
  21. Buchanan F, McReynolds M, Couvillon A, Kam Y, Holla V, DuBois R, et al. Requirement of phospholipase D1 activity in H-RasV12-induced transformation. Proc Natl Acad Sci U S A. 2005;102:1638-42 pubmed
    ..importance of phospholipase D in the oncogenic ability of Ras, we used Rat-2 fibroblasts with reduced phospholipase D1 activity (Rat-2V25)...
  22. Steed P, Clark K, Boyar W, Lasala D. Characterization of human PLD2 and the analysis of PLD isoform splice variants. FASEB J. 1998;12:1309-17 pubmed
    ..Recent advances in this field include the reports of cDNA sequences for two mammalian PLD isoforms: human PLD1 and rodent PLD2. We report the characterization of cDNA encoding human PLD2...
  23. Oka M, Kageshita T, Ono T, Goto A, Kuroki T, Ichihashi M. Protein kinase C alpha associates with phospholipase D1 and enhances basal phospholipase D activity in a protein phosphorylation-independent manner in human melanoma cells. J Invest Dermatol. 2003;121:69-76 pubmed
    ..To elucidate the role of phospholipase D in melanoma, the expression of phospholipase D1 and protein kinase C alpha in primary and metastatic lesions of acral lentiginous melanoma and superficial ..
  24. Divecha N, Roefs M, Halstead J, D Andrea S, Fernandez Borga M, Oomen L, et al. Interaction of the type Ialpha PIPkinase with phospholipase D: a role for the local generation of phosphatidylinositol 4, 5-bisphosphate in the regulation of PLD2 activity. EMBO J. 2000;19:5440-9 pubmed
    ..We find that both PLD1 and PLD2 interact with the Type Ialpha PIPkinase and that PLD2 activity in vivo can be regulated solely by the ..
  25. Park S, Han J. Phospholipase D1 Signaling: Essential Roles in Neural Stem Cell Differentiation. J Mol Neurosci. 2018;64:333-340 pubmed publisher
    b>Phospholipase D1 (PLD1) is generally accepted as playing an important role in the regulation of multiple cell functions, such as cell growth, survival, differentiation, membrane trafficking, and cytoskeletal organization...
  26. Dhingra S, Rodriguez M, Shen Q, Duan X, Stanton M, Chen L, et al. Constitutive activation with overexpression of the mTORC2-phospholipase D1 pathway in uterine leiomyosarcoma and STUMP: morphoproteomic analysis with therapeutic implications. Int J Clin Exp Pathol. 2010;4:134-46 pubmed
    ..study, we investigated the activation status and subcellular localization of mTOR and the relative expression of PLD1, as well as their downstream effectors in a spectrum of uterine smooth muscle tumors using normal myometria as ..
  27. Mateos M, Kamerbeek C, Giusto N, Salvador G. The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium. Int J Biochem Cell Biol. 2014;55:119-28 pubmed publisher
    ..In this work the role of phospholipase D (PLD) classical isoforms, PLD1 and PLD2, in the inflammatory response of human RPE cells (ARPE-19) was studied...
  28. Hu T, Liu Z, Shen X. Roles of phospholipase D in phorbol myristate acetate-stimulated neutrophil respiratory burst. J Cell Mol Med. 2011;15:647-53 pubmed publisher
    ..The results indicate that U73122 may act as an inhibitor of PLD, and PLD activation is required in PMA-stimulated respiratory burst. ..
  29. Torabi E, Behdani M, Chafi M, Moazzami R, Sabatier J, Khalaj V, et al. Characteristics and Lethality of a Novel Recombinant  Dermonecrotic Venom Phospholipase D from  Hemiscorpius lepturus. Toxins (Basel). 2017;9: pubmed publisher
    ..Sphingomyelinase, dermonecrotic and lethal activities were examined. Hl-PLD1 showed remarkable sequence similarity and structural homology with PLDs of brown spiders...
  30. Zhu M, Foreman D, O Brien S, Jin Y, Zhang W. Phospholipase D in TCR-Mediated Signaling and T Cell Activation. J Immunol. 2018;200:2165-2173 pubmed publisher
    ..In this study, we used mice deficient in PLD1, PLD2, or both to assess the function of these enzymes in T cells...
  31. Kwun H, Lee J, Min D, Jang K. Transcriptional repression of cyclin-dependent kinase inhibitor p21 gene by phospholipase D1 and D2. FEBS Lett. 2003;544:38-44 pubmed
    ..In this report, we demonstrated that both PLD1 and PLD2 repressed expression of cyclin-dependent kinase inhibitor p21 gene in an additive manner...
  32. Shemon A, Sluyter R, Wiley J. Rottlerin inhibits P2X(7) receptor-stimulated phospholipase D activity in chronic lymphocytic leukaemia B-lymphocytes. Immunol Cell Biol. 2007;85:68-72 pubmed
    ..Our results indicate a possible role for novel PKC isoforms in the regulation of P2X(7)-mediated PLD activity. ..
  33. Chakraborti S, Sarkar J, Chowdhury A, Chakraborti T. Role of ADP ribosylation factor6- Cytohesin1-PhospholipaseD signaling axis in U46619 induced activation of NADPH oxidase in pulmonary artery smooth muscle cell membrane. Arch Biochem Biophys. 2017;633:1-14 pubmed publisher
    ..The cell membrane contains predominantly PLD2 along with PLD1 isoforms of PLD...
  34. Henry F, Wang X, Serrano D, Perez A, Carruthers C, Stuenkel E, et al. A Unique Homeostatic Signaling Pathway Links Synaptic Inactivity to Postsynaptic mTORC1. J Neurosci. 2018;38:2207-2225 pubmed publisher
    ..Pharmacological blockade of PLD1/2 or pharmacogenetic disruption of PA interactions with mTOR eliminates mTORC1 signaling and presynaptic ..
  35. Usatyuk P, Gorshkova I, He D, Zhao Y, Kalari S, Garcia J, et al. Phospholipase D-mediated activation of IQGAP1 through Rac1 regulates hyperoxia-induced p47phox translocation and reactive oxygen species generation in lung endothelial cells. J Biol Chem. 2009;284:15339-52 pubmed publisher
    ..Infection of HPAECs with adenoviral constructs of PLD1 and PLD2 wild-type potentiated hyperoxia-induced PLD activation and accumulation of O(2)(...
  36. Yoon M. Vps34 and PLD1 take center stage in nutrient signaling: their dual roles in regulating autophagy. Cell Commun Signal. 2015;13:44 pubmed publisher
    ..Recent studies have shown that phospholipase PLD1, a downstream regulator of Vps34, is also closely involved in both mTOR activation and autophagy...
  37. Wang L, Cummings R, Usatyuk P, Morris A, Irani K, Natarajan V. Involvement of phospholipases D1 and D2 in sphingosine 1-phosphate-induced ERK (extracellular-signal-regulated kinase) activation and interleukin-8 secretion in human bronchial epithelial cells. Biochem J. 2002;367:751-60 pubmed
    ..Here we provide evidence that activation of phospholipases D1 and D2 (PLD1 and PLD2) by S1P regulates the phosphorylation of extracellular-signal-regulated kinase (ERK) and IL-8 secretion in ..
  38. Nagasaki A, Inotsume K, Kanada M, Uyeda T. Phospholipase D is essential for keratocyte-like migration of NBT-II cells. Cell Struct Funct. 2008;33:27-33 pubmed
    ..To investigate the functional difference between the two isozymes of PLD (PLD1 and PLD2), we transfected NBT-II cells with vectors expressing shRNA to deplete PLD1 or PLD2...
  39. Kranc W, Budna J, Chachuła A, Borys S, Bryja A, Rybska M, et al. "Cell Migration" Is the Ontology Group Differentially Expressed in Porcine Oocytes Before and After In Vitro Maturation: A Microarray Approach. DNA Cell Biol. 2017;36:273-282 pubmed publisher
    ..We found nine genes involved in cell migration mechanisms, that is, PLD1, KIT, LAMA2, MAP3K1, VEGFA, TGFBR3, INSR, TPM1, and RTN4...
  40. Riebeling C, Bourgoin S, Shields D. Caspase cleavage of phospholipase D1 in vitro alters its regulation and reveals a novel property of the "loop" region. Biochim Biophys Acta. 2008;1781:376-82 pubmed publisher
    ..To test this idea, we have used in vitro translation of PLD1 and PLD2 which generated active enzymes exhibiting properties mimicking those of the endogenous proteins...
  41. Wang J, Wang N, Xie J, Walton S, McKown R, Raab R, et al. Restricted epithelial proliferation by lacritin via PKCalpha-dependent NFAT and mTOR pathways. J Cell Biol. 2006;174:689-700 pubmed
    ..Outward flow of lacritin from secretory cells through ducts may generate a proliferative/secretory field as a different unit of cellular renewal in nongermative epithelia where luminal structures predominate. ..
  42. Kim J, Choi H, Oh C, Oh J, Han J. PLD1 activation mediates Amb a 1-induced Th2-associated cytokine expression via the JNK/ATF-2 pathway in BEAS-2B cells. Cell Immunol. 2015;298:9-17 pubmed publisher
    The purpose of this study was to identify the role of phospholipase D1 (PLD1) in Amb a 1-induced IL-5 and IL-13 expression...
  43. Han S, Huh J, Kim W, Jeong S, Min D, Jung Y. Phospholipase D activates HIF-1-VEGF pathway via phosphatidic acid. Exp Mol Med. 2014;46:e126 pubmed publisher
    ..In this study, a potential mechanism for PLD-mediated tumorigenesis was explored. Ectopic expression of PLD1 or PLD2 in human glioma U87 cells increased the expression of hypoxia-inducible factor-1α (HIF-1α) protein...
  44. Huang J, Haberichter S, Sadler J. The B subunits of Shiga-like toxins induce regulated VWF secretion in a phospholipase D1-dependent manner. Blood. 2012;120:1143-9 pubmed publisher
    ..Inhibition of PLD activity or shRNA-mediated PLD1 knockdown abolished StxB-induced VWF secretion...
  45. Friday S, Fox D. Phospholipase D enzymes facilitate IL-17- and TNF?-induced expression of proinflammatory genes in rheumatoid arthritis synovial fibroblasts (RASF). Immunol Lett. 2016;174:9-18 pubmed publisher
    ..Microarray analysis of RASF showed that phospholipase D1 (PLD1) is among genes significantly induced by IL-17...
  46. Behera D, Behera P, Acharya L, Dixit A. Pharmacophore modelling, virtual screening and molecular docking studies on PLD1 inhibitors. SAR QSAR Environ Res. 2017;:1-19 pubmed publisher
    ..governing lipid metabolism and viral replication is not properly understood to date, but both Phospholipase D (PLD1 and PLD2) activities are stimulated in viral infection...
  47. Barber C, Huganir R, Raben D. Phosphatidic acid-producing enzymes regulating the synaptic vesicle cycle: Role for PLD?. Adv Biol Regul. 2018;67:141-147 pubmed publisher
    ..2016). In addition to this enzyme, studies implicate PLD1 in neurotransmission, although its precise role is of some debate...
  48. Wakelam M, McNee G, Rudge S. Cross-talk between phospholipase D and MAP kinases activities. Adv Enzyme Regul. 2010;50:57-61 pubmed publisher
  49. Lee C, Kim S, Chung J, Frohman M, Kilimann M, Rhee S. Inhibition of phospholipase D by amphiphysins. J Biol Chem. 2000;275:18751-8 pubmed
    ..Bacterially expressed recombinant amphiphysins inhibited both PLD1 and PLD2 isozymes in vitro with a potency similar to that of brain amphiphysin (median inhibitory concentration of ..
  50. Becker K, Hannun Y. Isoenzyme-specific translocation of protein kinase C (PKC)betaII and not PKCbetaI to a juxtanuclear subset of recycling endosomes: involvement of phospholipase D. J Biol Chem. 2004;279:28251-6 pubmed
    ..Taken together, these results define specific biochemical and cellular actions of PKCbetaII when compared with PKCbetaI. ..
  51. Kim Y, Han J, Park J, Lee S, Oh Y, Chung C, et al. Phosphorylation and activation of phospholipase D1 by protein kinase C in vivo: determination of multiple phosphorylation sites. Biochemistry. 1999;38:10344-51 pubmed
    Protein kinase C (PKC) is an important regulator of phospholipase D1 (PLD1). Currently there is some controversy about a phosphorylation-dependent or -independent mechanism of the activation of PLD1 by PKC...
  52. Cai D, Netzer W, Zhong M, Lin Y, Du G, Frohman M, et al. Presenilin-1 uses phospholipase D1 as a negative regulator of beta-amyloid formation. Proc Natl Acad Sci U S A. 2006;103:1941-6 pubmed
    ..Here we demonstrate that PS1, through its loop region, binds to phospholipase D1 (PLD1), thereby recruiting it to the Golgi/trans-Golgi network...
  53. Taylor H, Simmons G, Mathews T, Khatua A, Popik W, Lindsley C, et al. Phospholipase D1 Couples CD4+ T Cell Activation to c-Myc-Dependent Deoxyribonucleotide Pool Expansion and HIV-1 Replication. PLoS Pathog. 2015;11:e1004864 pubmed publisher
    ..Here, we show that phospholipase D1 (PLD1) links T cell activation signals to increased HIV-1 permissivity by triggering a c-Myc-dependent ..
  54. Song O, Queval C, Iantomasi R, Delorme V, Marion S, Veyron Churlet R, et al. ArfGAP1 restricts Mycobacterium tuberculosis entry by controlling the actin cytoskeleton. EMBO Rep. 2018;19:29-42 pubmed publisher
    ..rearrangements of the actin cytoskeleton, which are regulated by ADP-ribosylation factor 1 (Arf1) and phospholipase D1 (PLD1), and is dependent on the M3 muscarinic receptor (M3R)...
  55. Cipriano R, Bryson B, Miskimen K, Bartel C, Hernandez Sanchez W, Bruntz R, et al. Hyperactivation of EGFR and downstream effector phospholipase D1 by oncogenic FAM83B. Oncogene. 2014;33:3298-306 pubmed publisher
    ..We report here that elevated FAM83B expression increases Phospholipase D (PLD) activity, and that suppression of PLD1 activity prevents FAM83B-mediated transformation...
  56. Bae C, Min D, Fleming I, Exton J. Determination of interaction sites on the small G protein RhoA for phospholipase D. J Biol Chem. 1998;273:11596-604 pubmed
  57. Yin H, Gui Y, Du G, Frohman M, Zheng X. Dependence of phospholipase D1 multi-monoubiquitination on its enzymatic activity and palmitoylation. J Biol Chem. 2010;285:13580-8 pubmed publisher
    ..In the present study, we show that PLD1, but not PLD2, is posttranslationally modified by multi-monoubiquitination...
  58. Powner D, Hodgkin M, Wakelam M. Antigen-stimulated activation of phospholipase D1b by Rac1, ARF6, and PKCalpha in RBL-2H3 cells. Mol Biol Cell. 2002;13:1252-62 pubmed
    ..Therefore, because inactive, dominant negative GTPases do not activate PLD1b, we propose that activation results from phosphatidylinositol 3-kinase-dependent stimulation of Rac1, ARF6, and PKCalpha. ..
  59. Ta Shma A, Zhang K, Salimova E, Zernecke A, Sieiro Mosti D, Stegner D, et al. Congenital valvular defects associated with deleterious mutations in the PLD1 gene. J Med Genet. 2017;54:278-286 pubmed publisher
    ..of the findings was assessed by studying sporulation of yeast carrying a homologous Phospholipase D (PLD1) mutation, in situ hybridisation in chick embryo and echocardiography and histological examination of hearts of ..
  60. Chen F, Ghosh A, Shneider B. Phospholipase D2 mediates signaling by ATPase class I type 8B membrane 1. J Lipid Res. 2013;54:379-85 pubmed publisher
    ..Activation of BSEP by FIC1 gain of function was blocked when PLD2 but not PLD1 was silenced...
  61. Yoon M, Koo J, Jeong Y, Kim Y, Lee J, Yun H, et al. Phospholipase D1 as a key enzyme for decidualization in human endometrial stromal cells. Biol Reprod. 2007;76:250-8 pubmed
    ..Simultaneously, the activity and expression levels of PLD1 increased...
  62. Carrigan S, Pink D, Stadnyk A. Neutrophil transepithelial migration in response to the chemoattractant fMLP but not C5a is phospholipase D-dependent and related to the use of CD11b/CD18. J Leukoc Biol. 2007;82:1575-84 pubmed
    ..Moreover, as PLD controls other neutrophil functions, such as the oxidative response, degranulation, and protease release, we could exclude these functions as being important in neutrophil transepithelial migration to C5a. ..
  63. Ghelli A, Porcelli A, Facchini A, Hrelia S, Flamigni F, Rugolo M. Phospholipase D1 is threonine-phosphorylated in human-airway epithelial cells stimulated by sphingosine-1-phosphate by a mechanism involving Src tyrosine kinase and protein kinase Cdelta. Biochem J. 2002;366:187-93 pubmed
    ..Finally, the level of serine phosphorylation of PLD1 and PLD2 isoforms was not changed, whereas the PLD1 isoform alone was threonine-phosphorylated in SPP-treated cells...
  64. Cai M, He J, Xiong J, Tay L, Wang Z, Rog C, et al. Phospholipase D1-regulated autophagy supplies free fatty acids to counter nutrient stress in cancer cells. Cell Death Dis. 2016;7:e2448 pubmed publisher
    ..Here we report that phospholipase D1 (PLD1) participates in the regulation of metabolic plasticity in cancer cells...
  65. Tenconi P, Giusto N, Salvador G, Mateos M. Phospholipase D1 modulates protein kinase C-epsilon in retinal pigment epithelium cells during inflammatory response. Int J Biochem Cell Biol. 2016;81:67-75 pubmed publisher
    ..with selective phospholipase D inhibitors demonstrated that protein kinase C-? phosphorylation depends on phospholipase D1 and 2 while protein kinase C-? activation depends only on phospholipase D1...
  66. Choi H, Park S, Cho J, Park J, Sohn J, Kim Y, et al. The TLR4-associated phospholipase D1 activation is crucial for Der f 2-induced IL-13 production. Allergy. 2015;70:1569-79 pubmed publisher
    ..p38 MAPK is then activated by PKCα and stimulates PLD1 activity by phosphorylating the Thr147 residue of PLD1...
  67. Kim S, Ahn B, Min K, Lee Y, Joe E, Min D. Phospholipase D isozymes mediate epigallocatechin gallate-induced cyclooxygenase-2 expression in astrocyte cells. J Biol Chem. 2004;279:38125-33 pubmed
    ..COX-2 expression was provided by the observations that COX-2 expression was stimulated by overexpression of PLD1 or PLD2 isozymes and treatment with phosphatidic acid (PA), and that prevention of PA dephosphorylation by 1-..
  68. Lopez I, Arnold R, Lambeth J. Cloning and initial characterization of a human phospholipase D2 (hPLD2). ADP-ribosylation factor regulates hPLD2. J Biol Chem. 1998;273:12846-52 pubmed
    ..b>PLD1, first cloned from human, is activated by small GTPases such as ADP-ribosylation factor (ARF) and RhoA...
  69. Kang D, Lee S, Yoon J, Park W, Choi K, Min D. Phospholipase D1 drives a positive feedback loop to reinforce the Wnt/beta-catenin/TCF signaling axis. Cancer Res. 2010;70:4233-42 pubmed publisher
    ..b>PLD1 expression and activity was enhanced by treatment with Wnt3a and glycogen synthase kinase-3 inhibitors, and the Wnt ..
  70. Pye D, Rubio I, Pusch R, Lin K, Pettitt A, Till K. Chemokine unresponsiveness of chronic lymphocytic leukemia cells results from impaired endosomal recycling of Rap1 and is associated with a distinctive type of immunological anergy. J Immunol. 2013;191:1496-504 pubmed publisher
    ..Finally, we show that failure of Rap1 translocation/GTP loading is linked to defective activation of phospholipase D1 and its upstream activator Arf1...
  71. Barclay Z, Dickson L, Robertson D, Johnson M, Holland P, Rosie R, et al. Attenuated PLD1 association and signalling at the H452Y polymorphic form of the 5-HT(2A) receptor. Cell Signal. 2013;25:814-21 pubmed publisher
    ..attenuated PLD signalling, which as in the wild-type receptor, was mediated by a molecular complex involving PLD1 docked to the receptor's carboxy-terminal tail domain...
  72. Cheng M, Rizwan A, Jiang L, Bhujwalla Z, Glunde K. Molecular Effects of Doxorubicin on Choline Metabolism in Breast Cancer. Neoplasia. 2017;19:617-627 pubmed publisher
    ..Of the two phosphatidylcholine-specific PLD enzymes, only PLD1, but not PLD2, mRNA was down-regulated by doxorubicin treatment...
  73. Zhang Q, Wang D, Singh N, Kundumani Sridharan V, Gadiparthi L, Rao C, et al. Activation of cytosolic phospholipase A2 downstream of the Src-phospholipase D1 (PLD1)-protein kinase C ? (PKC?) signaling axis is required for hypoxia-induced pathological retinal angiogenesis. J Biol Chem. 2011;286:22489-98 pubmed publisher
    ..vascular endothelial growth factor (VEGF)-induced pathological retinal angiogenesis requires the activation of Src-PLD1-PKC? signaling...
  74. Yoon M, Du G, Backer J, Frohman M, Chen J. Class III PI-3-kinase activates phospholipase D in an amino acid-sensing mTORC1 pathway. J Cell Biol. 2011;195:435-47 pubmed publisher
    ..In this study, we identify a novel role for PLD1 in an amino acid-sensing pathway...
  75. Roth E, Frohman M. Proliferative and metastatic roles for Phospholipase D in mouse models of cancer. Adv Biol Regul. 2018;67:134-140 pubmed publisher
    ..The canonical enzymes PLD1 and PLD2 enact their diverse effects through hydrolyzing the membrane lipid phosphatidylcholine to generate the ..
  76. Olala L, Seremwe M, Tsai Y, Bollag W. A role for phospholipase D in angiotensin II-induced protein kinase D activation in adrenal glomerulosa cell models. Mol Cell Endocrinol. 2013;366:31-7 pubmed publisher
    ..These results indicate that PKD is downstream of PLD and suggest that PKD is one of the mechanisms through which PLD promotes aldosterone production in response to AngII in adrenal glomerulosa cells. ..
  77. Natarajan V, Scribner W, Morris A, Roy S, Vepa S, Yang J, et al. Role of p38 MAP kinase in diperoxovanadate-induced phospholipase D activation in endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2001;281:L435-49 pubmed
    ..Immunoprecipitation of PLD from EC lysates with PLD1 and PLD2 antibodies revealed both PLD isoforms associated with p38 MAPK...
  78. Min D, Ahn B, Jo Y. Differential tyrosine phosphorylation of phospholipase D isozymes by hydrogen peroxide and the epidermal growth factor in A431 epidermoid carcinoma cells. Mol Cells. 2001;11:369-78 pubmed
    The regulatory mechanism through which the phospholipase D (PLD) isoforms PLD1 and PLD2 are activated is poorly understood...
  79. Eftekharian M, Azimi T, Ghafouri Fard S, Sayad A, Omrani M, Sarrafzadeh S, et al. Phospholipase D1 expression analysis in relapsing-remitting multiple sclerosis patients. Neurol Sci. 2017;38:865-872 pubmed publisher
    ..b>Phospholipase D1 (PLD1) is a critical enzyme responsible for the making lipid second messenger phosphatidic acid...
  80. Harel Dassa K, Yedgar S, Trope C, Davidson B, Reich R. Phospholipase D messenger RNA expression and clinical role in high-grade serous carcinoma. Hum Pathol. 2017;62:115-121 pubmed publisher
    ..b>PLD1 and PLD2 isoform expression was studied in 125 HGSC specimens (73 effusions, 28 ovarian tumors, 24 solid metastases)..
  81. Lopez C, Pelletán L, Suhaiman L, De Blas G, Vitale N, Mayorga L, et al. Diacylglycerol stimulates acrosomal exocytosis by feeding into a PKC- and PLD1-dependent positive loop that continuously supplies phosphatidylinositol 4,5-bisphosphate. Biochim Biophys Acta. 2012;1821:1186-99 pubmed publisher
    ..Addition of diacylglycerol promotes the PKC-dependent activation of PLD1. Rescue experiments adding phosphatidic acid or PIP(2) and direct measurement of lipid production suggest that both ..