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| PLCZ1SummaryGene Symbol: PLCZ1 Description: phospholipase C, zeta 1 Alias: NYD-SP27, PLC-zeta-1, PLCzeta, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1, PI-phospholipase C zeta 1, phosphoinositide phospholipase C-zeta-1, testis-development related NYD-SP27 Species: human Top Publications
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Publications
Microinjection of mouse phospholipase C zeta complementary RNA into mare oocytes induces long-lasting intracellular calcium oscillations and embryonic developmentSylvia J Bedford-Guaus
Department of Clinical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY 14853, USA
Reprod Fertil Dev 20:875-83. 2008..Because phospholipase Czeta (PLCzeta) is the postulated sperm-borne factor responsible for oocyte activation at fertilisation, the aim of the present ..
Human sperm devoid of PLC, zeta 1 fail to induce Ca(2+) release and are unable to initiate the first step of embryo developmentSook Young Yoon
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts, USA
J Clin Invest 118:3671-81. 2008..We have also shown that PLC, zeta 1 (PLCZ1), the sperm protein thought to induce [Ca(2+)](i) oscillations, was localized to the equatorial region of wild-..- Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytesL J Cox
Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
Reproduction 124:611-23. 2002..A sperm-specific zeta isoform of phospholipase C (PLCzeta) has been identified in mice... - Human immunodeficiency virus-1 glycoproteins gp120 and gp160 specifically inhibit the CD3/T cell-antigen receptor phosphoinositide transduction pathwayD Cefai
Laboratoire d Immunologie Cellulaire et Tissulaire, Group Hospitalier GH Pitié Salpétrière, Paris, France
J Clin Invest 86:2117-24. 1990.... - PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo developmentChristopher M Saunders
Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
Development 129:3533-44. 2002..Our results are consistent with sperm PLC zeta as the molecular trigger for development of a fertilised egg into an embryo... - Relationship between nuclear sequestration of PLCzeta and termination of PLCzeta-induced Ca2+ oscillations in mouse eggsMasahiko Ito
Department of Physiology, Tokyo Women s Medical University School of Medicine, Shinjuku ku, Tokyo 162 8666, Japan
Cell Calcium 44:400-10. 2008Phospholipase C-zeta (PLCzeta), a strong candidate of the egg-activating sperm factor, causes long-lasting series of Ca2+ spikes and thereby egg activation when expressed in mouse eggs by injection of cRNA... 
Use of luciferase chimaera to monitor PLCzeta expression in mouse eggsKarl Swann
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
Methods Mol Biol 518:17-29. 2009..The PLCzeta protein that is expressed in eggs after injection of cRNA is effective in causing Ca2+ oscillations at very low ..- The pattern of localization of the putative oocyte activation factor, phospholipase Czeta, in uncapacitated, capacitated, and ionophore-treated human spermatozoaP Grasa
Department of Pharmacology, University of Oxford, Oxford, UK
Hum Reprod 23:2513-22. 2008Recent studies suggest that in mammals, oocyte activation at fertilization is triggered by a sperm-specific phospholipase C, PLCzeta. We investigated PLCzeta localization in human spermatozoa. - The dynamics of calcium oscillations that activate mammalian eggsKarl Swann
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
Int J Dev Biol 52:585-94. 2008..b>PLCzeta is stimulated by low intracellular calcium levels in a manner which suggests that there is a regenerative .. - Birth of normal offspring from mouse eggs activated by a phospholipase Czeta protein lacking three EF-hand domainsTomoko Nakanishi
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
J Reprod Dev 54:244-9. 2008Sperm-specific phospholipase C, PLCzeta, is a candidate for the Ca(2+) oscillation-inducing factor that is introduced into the ooplasm upon sperm-egg fusion... - Regulation of diacylglycerol production and protein kinase C stimulation during sperm- and PLCzeta-mediated mouse egg activationYuansong Yu
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, U K
Biol Cell 100:633-43. 2008..Increased inositol 1,4,5-trisphosphate production appears to be triggered by a sperm-derived PLCzeta (phospholipase C-zeta) that enters the egg after gamete fusion... - Testis-specific novel transcripts in chicken: in situ localization and expression pattern profiling during sexual developmentDeivendran Rengaraj
Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea
Biol Reprod 79:413-20. 2008..Among the testis-specific TC sequences, TC120901 is a known gene, phospholipase C, zeta (PLCZ1)... - Difference in Ca2+ oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, between mouse, rat, human, and medaka fishMasahiko Ito
Department of Physiology, Tokyo Women s Medical University School of Medicine, Shinjuku ku, Tokyo 162 8666, Japan
Biol Reprod 78:1081-90. 2008Mouse phospholipase C, zeta 1 (PLCZ1), a strong candidate of egg-activating sperm factor, induces Ca(2+) oscillations and accumulates into formed pronucleus (PN) when expressed by cRNA injection... - Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zetaPablo J Ross
Cellular Reprogramming Laboratory, Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA
BMC Dev Biol 8:16. 2008..b>PLCZ1 has been identified as the factor that the sperm delivers into the egg to induce such a response... - NYD-SP27, a novel intrinsic decapacitation factor in spermYe Bi
Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China
Asian J Androl 11:229-39. 2009..In this study, we report that NYD-SP27, an isoform of phospholipase C Zeta 1 (PLCZ1), is localized to the sperm acrosome in mouse and human spermatozoa by immunofluorescence using a specific .. - Effects of liquid preservation of sperm on their ability to activate oocytes and initiate preimplantational development after intracytoplasmic sperm injection in the pigN T Binh
Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
Theriogenology 71:1440-50. 2009..then damage to the plasma membrane, morphologic changes of the acrosome, and the amount of phospholipase Czeta (PLCzeta) in the sperm were assessed by SYBR-14/propidium iodide staining, fluorescein isothiocyanate-conjugated peanut .. - A maternally inherited autosomal point mutation in human phospholipase C zeta (PLCζ) leads to male infertilityJunaid Kashir
Nuffield Department of Obstetrics and Gynaecology, Level 3, Women s Centre, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK
Hum Reprod 27:222-31. 2012..a process normally regulated by calcium oscillations, thought to be induced by a sperm-specific phospholipase C, PLCzeta (PLCζ)... - Could modifications of signalling pathways activated after ICSI induce a potential risk of epigenetic defects?Brigitte Ciapa
UMR CNRS 8195 Centre de Neurosciences Paris Sud CNPS, Universite Paris XI, Orsay Cedex, France
Int J Dev Biol 55:143-52. 2011..Among the many potential sperm factors, PLCzeta is the strongest bona fide sperm factor candidate... - Identification and functional analysis of an ovarian form of the egg activation factor phospholipase C zeta (PLCζ) in pufferfishKevin Coward
Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, Oxford, UK
Mol Reprod Dev 78:48-56. 2011Recent studies suggest that egg activation in mammals is triggered by a sperm-specific phospholipase C, PLCzeta. In other vertebrate species such as medaka fish, chickens, and quail, PLCzeta is also expressed as a testis-specific mRNA... - Male infertility-linked point mutation disrupts the Ca2+ oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζMichail Nomikos
Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
Biochem J 434:211-7. 2011..Wild-type PLCζ initiated a normal pattern of Ca2+ oscillations in eggs in the presence of 10-fold higher mutant PLCζ, suggesting that infertility is not mediated by a dominant-negative mechanism... - Reduced fertilization after ICSI and abnormal phospholipase C zeta presence in spermatozoa from the wobbler mouseElke Heytens
Department of Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, B 9000 Ghent, Belgium
Reprod Biomed Online 21:742-9. 2010..The presence of PLCzeta in the spermatozoa and testis of the wobbler mouse was evaluated by PLCzeta immunostaining and quantitative .. - Species-specific differences in the activity and nuclear localization of murine and bovine phospholipase C zeta 1Melissa A Cooney
Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
Biol Reprod 83:92-101. 2010Injection of mammalian sperm extracts or cRNA of the sperm-specific phospholipase C zeta 1 (PLCZ1) has been shown to trigger repetitive oscillations in the concentration of free calcium ([Ca(2+)](i)), leading to oocyte activation and .. - Complete globozoospermia associated with PLCζ deficiency treated with calcium ionophore and ICSI results in pregnancyS L Taylor
The Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, VA, USA
Reprod Biomed Online 20:559-64. 2010..transmission electron microscopy, Western blotting and immunofluorescence for detection of phospholipase C zeta (PLCzeta), as well as ICSI outcome, of an affected patient... - Phospholipase Czeta mRNA expression and its potency during spermatogenesis for activation of quail oocyte as a sperm factorShusei Mizushima
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, Japan
Mol Reprod Dev 76:1200-7. 2009..First, effects of a microinjection of quail sperm extract (SE) and quail phospholipase Czeta (PLCzeta) cRNA into quail oocytes were assessed by observation of pronuclear formation and cytoplasmic segmentation, .. - Reduced amounts and abnormal forms of phospholipase C zeta (PLCzeta) in spermatozoa from infertile menE Heytens
Department of Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium
Hum Reprod 24:2417-28. 2009..In mammals, oocyte activation at fertilization is thought to be induced by the sperm-specific phospholipase C zeta (PLCzeta). However, it still remains to be conclusively shown that PLCzeta is the endogenous agent of oocyte activation... - Effect of sperm extract injection volume, injection of PLCzeta cRNA, and tissue cell line on efficiency of equine nuclear transferYoung Ho Choi
College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843 4466, USA
Cloning Stem Cells 11:301-8. 2009..2 sec (0%) or 0.8 sec (1.4%). In Experiment 2, injection of murine PLCzeta cRNA before or after ionomycin treatment did not increase blastocyst development (0 and 4... - Removal of acrosomal membrane from sperm head improves development of rat zygotes derived from intracytoplasmic sperm injectionYasunari Seita
Laboratory of Animal Reproduction, Graduate School of Veterinary Science, Azabu University, Sagamihira, Japan
J Reprod Dev 55:475-9. 2009..05). However, neither TX nor LL treatment affected amounts of PLCzeta in rat sperm. The rates of offspring derived from TX- (20.3 +/- 4.4%) and LL-treated sperm (19.0 +/- 2... - Proteolytic processing of phospholipase Czeta and [Ca2+]i oscillations during mammalian fertilizationManabu Kurokawa
Paige Laboratory, Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA
Dev Biol 312:407-18. 2007Phospholipase Czeta (PLCzeta) is a sperm-specific PLC capable of causing repetitive intracellular Ca2+ ([Ca2+]i) release ([Ca2+]i oscillations) in mammalian eggs... - Preimplantation development of mouse oocytes activated by different levels of human phospholipase C zetaY Yu
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
Hum Reprod 23:365-73. 2008A sperm-specific phospholipase C zeta (PLCzeta) has been shown to trigger Ca(2+) oscillations in mouse and human oocytes and appears to be the sperm factor responsible for activation at fertilization... - Functional, biochemical, and chromatographic characterization of the complete [Ca2+]i oscillation-inducing activity of porcine spermManabu Kurokawa
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA
Dev Biol 285:376-92. 2005..Recent evidence shows that the novel sperm-specific phospholipase C (PLC), PLCzeta, may be the long sought after [Ca2+]i oscillation-inducing SF... - Phospholipase Czeta, the trigger of egg activation in mammals, is present in a non-mammalian speciesK Coward
Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
Reproduction 130:157-63. 2005..but recent studies suggest that, in mammals, egg activation is triggered by a testis-specific phospholipase C: PLCzeta. Since the discovery of PLCzeta, it has been unclear whether its role in triggering egg activation is common to .. - Role of phospholipase C-zeta domains in Ca2+-dependent phosphatidylinositol 4,5-bisphosphate hydrolysis and cytoplasmic Ca2+ oscillationsMichail Nomikos
Cell Signalling Laboratory, Wales Heart Research Institute, School of Medicine, Cardiff University, Cardiff CF14 4XN
J Biol Chem 280:31011-8. 2005The sperm-specific phospholipase C-zeta (PLCzeta) elicits fertilization-like Ca2+ oscillations and activation of embryo development when microinjected into mammalian eggs (Saunders, C. M., Larman, M. G., Parrington, J., Cox, L. J... - Nuclear translocation of phospholipase C-zeta, an egg-activating factor, during early embryonic developmentYoshie Sone
Department of Physiology, Tokyo Women s Medical University School of Medicine, Tokyo 162 8666, Japan
Biochem Biophys Res Commun 330:690-4. 2005Phospholipase C-zeta (PLCzeta), a strong candidate of the egg-activating sperm factor, causes intracellular Ca2+ oscillations and egg activation, and is subsequently accumulated into the pronucleus (PN), when expressed in mouse eggs by .. - The role of EF-hand domains and C2 domain in regulation of enzymatic activity of phospholipase CzetaZen Kouchi
Department of Physiology, Tokyo Women s Medical University School of Medicine, Shinjuku ku, Tokyo 162 8666, Japan
J Biol Chem 280:21015-21. 2005Sperm-specific phospholipase C-zeta (PLCzeta) induces Ca2+ oscillations and egg activation when injected into mouse eggs... - Transgenic RNA interference reveals role for mouse sperm phospholipase Czeta in triggering Ca2+ oscillations during fertilizationJason G Knott
Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
Biol Reprod 72:992-6. 2005A sperm-specific phospholipase (PL) C, termed PLCzeta, is proposed to be the soluble sperm factor that induces Ca(2+) oscillations in mammalian eggs and, thus, initiates egg activation in vivo... - Mammalian phospholipase Czeta induces oocyte activation from the sperm perinuclear matrixSatoko Fujimoto
Laboratory of Mammalian Molecular Embryology, RIKEN Center for Developmental Biology, Chuo Ku, Kobe 650 0047, Japan
Dev Biol 274:370-83. 2004..Phospholipase C-zeta (PLCzeta) correlated absolutely with activating ability... - Cell cycle-dependent Ca2+ oscillations in mouse embryos are regulated by nuclear targeting of PLCzetaMark G Larman
Department of Anatomy and Developmental Biology, University College London, WC1E 6BT, UK
J Cell Sci 117:2513-21. 2004..We recently identified a novel, sperm-specific phospholipase C (PLCzeta; PLCzeta) that triggers Ca2+ oscillations similar to those caused by sperm... - The cytosolic sperm factor that triggers Ca2+ oscillations and egg activation in mammals is a novel phospholipase C: PLCzetaK Swann
Department of Anatomy and Developmental Biology, University College London, London WC1E 6BT, UK
Reproduction 127:431-9. 2004..shown that this soluble sperm factor protein is a novel form of phospholipase C (PLC), and it is referred to as PLCzeta(zeta)... - Recombinant phospholipase Czeta has high Ca2+ sensitivity and induces Ca2+ oscillations in mouse eggsZen Kouchi
Department of Physiology, Tokyo Women s Medical University School of Medicine, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
J Biol Chem 279:10408-12. 2004Sperm-specific phospholipase Czeta (PLCzeta) is known to induce intracellular Ca(2+) oscillations and subsequent early embryonic development when expressed in mouse eggs by injection of RNA encoding PLCzeta (Saunders, C. M., Larman, M. G... - The latest phospholipase C, PLCeta, is implicated in neuronal functionShamshad Cockcroft
Department of Physiology, University College London, London WC1E 6JJ, UK
Trends Biochem Sci 31:4-7. 2006..into five families designated PLCbeta (1-4), PLCgamma (1 and 2), PLCdelta (1, 3 and 4), PLCepsilon (1) and PLCzeta (1)... - Mammalian egg activation: from Ca2+ spiking to cell cycle progressionKeith T Jones
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
Reproduction 130:813-23. 2005..pathway remain to be fully investigated, here I present a model in which a sperm-specific phospholipase C (PLCzeta) generates Ca(2+) spikes to activate calmodulin-dependent protein kinase II and so switch on the Anaphase-.. - Expression of a fluorescent recombinant form of sperm protein phospholipase C zeta in mouse epididymal sperm by in vivo gene transfer into the testisKevin Coward
Department of Pharmacology, University of Oxford, Oxford, United Kingdom
Fertil Steril 85:1281-9. 2006..To use in vivo gene transfer into the testis by electroporation to express a fluorescent recombinant form of a testis-specific gene in the mature epididymal sperm of mice and thus study the pattern of gene localization... - Release of phospholipase C zetaand [Ca2+]i oscillation-inducing activity during mammalian fertilizationSook Young Yoon
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts 01003, USA
Reproduction 134:695-704. 2007..We also stained the enucleated sperm heads to ascertain the presence/absence of PLCzeta. Our results show that by 90 min all the SF activity had been released from the sperm, as fertilized enucleated .. - Alterations of PLCbeta1 in mouse eggs change calcium oscillatory behavior following fertilizationHideki Igarashi
Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia, PA 19104, USA
Dev Biol 312:321-30. 2007..Much attention currently focuses on the role of sperm-derived PLCzeta in generating changes in egg intracellular Ca2+ despite the fact that PLCzeta constitutes a very small fraction .. - Broad, ectopic expression of the sperm protein PLCZ1 induces parthenogenesis and ovarian tumours in miceNaoko Yoshida
Laboratory of Mammalian Molecular Embryology, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
Development 134:3941-52. 2007..are induced at fertilisation by a signal thought to come from the sperm protein, phospholipase C-zeta (PLCZ1)... - Intracellular calcium in the fertilization and development of mammalian eggsKeith T Jones
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, UK
Clin Exp Pharmacol Physiol 34:1084-9. 2007..3. Ca(2+) spiking is also observed in mitosis of one-cell embryos, probably because PLCzeta contains a nuclear localization signal and so is released into the cytoplasm following nuclear envelope breakdown... - Effect of NYD-SP27 down-regulation on ATP-induced Ca2+-dependent pancreatic duct anion secretion in cystic fibrosis cellsJin Xia Zhu
Epithelial Cell Biology Research Center, Department of Physiology, Faculty of Medicine, Rm 410, Basic Medical Science Building, The Chinese University of Hong Kong, Shatin, Hong Kong, China
Cell Biol Int 31:521-5. 2007.... - Molecular cloning, testicular postnatal expression, and oocyte-activating potential of porcine phospholipase CzetaAkihiro Yoneda
Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
Reproduction 132:393-401. 2006..Recently, oocyte activation has been shown to be induced by sperm-specific phospholipase Czeta (PLCzeta). The ability of PLCzeta to induce oocyte activation is highly conserved across vertebrates... - The role of X/Y linker region and N-terminal EF-hand domain in nuclear translocation and Ca2+ oscillation-inducing activities of phospholipase Czeta, a mammalian egg-activating factorKeiji Kuroda
Department of Physiology, Tokyo Women s Medical University School of Medicine, Tokyo 162 8666, Japan
J Biol Chem 281:27794-805. 2006Sperm-specific phospholipase C-zeta (PLCzeta) causes intracellular Ca(2+) oscillations and thereby egg activation and is accumulated into the formed pronucleus (PN) when expressed in mouse eggs by injection of cRNA encoding PLCzeta, .. - Calcium signals for egg activation in mammalsShunichi Miyazaki
Department of Physiology, Tokyo Women s Medical University School of Medicine, Japan
J Pharmacol Sci 100:545-52. 2006..candidate of the sperm factor is a novel sperm-specific isozyme of phospholipase C (IP(3)-producing enzyme), PLCzeta. Recent extensive research has reveled characteristics of PLCzeta such as the Ca(2+) oscillation-inducing .. - PLCzeta(zeta): a sperm protein that triggers Ca2+ oscillations and egg activation in mammalsK Swann
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
Semin Cell Dev Biol 17:264-73. 2006..We recently identified a sperm-specific form of phospholipase C (PLC), referred to as PLCzeta(zeta)... - Measurement of intracellular IP3 during Ca2+ oscillations in mouse eggs with GFP-based FRET probeHideki Shirakawa
Department of Applied Physics and Chemistry, The University of Electro Communications, Tokyo 182 8585, Japan
Biochem Biophys Res Commun 345:781-8. 2006..protein variants, during Ca2+ oscillations induced by fertilization or expression of phospholipase Czeta (PLCzeta), an egg-activating sperm factor candidate... - Teleost fish spermatozoa contain a cytosolic protein factor that induces calcium release in sea urchin egg homogenates and triggers calcium oscillations when injected into mouse oocytesKevin Coward
Department of Pharmacology, University of Oxford, Oxford OX1 3QT, UK
Biochem Biophys Res Commun 305:299-304. 2003..identified this sperm-derived factor as being a novel sperm-specific PLC isoform with distinctive properties (PLCzeta). Homologues of PLCzeta have since been isolated from human and cynomolgus monkey sperm...
Research Grants
- ELECTROSTATIC POTENTIALS AND BIOLOGICAL MEMBRANESStuart McLaughlin; Fiscal Year: 2007..unstructured basic clusters provide sufficient specificity for PIP2 to target proteins such as the sperm factor PLCzeta to the plasma membrane...