PLCZ1

Summary

Gene Symbol: PLCZ1
Description: phospholipase C, zeta 1
Alias: NYD-SP27, PLC-zeta-1, PLCzeta, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1, PI-phospholipase C zeta 1, phosphoinositide phospholipase C-zeta-1, testis-development related NYD-SP27
Species: human

Top Publications

  1. ncbi Microinjection of mouse phospholipase C zeta complementary RNA into mare oocytes induces long-lasting intracellular calcium oscillations and embryonic development
    Sylvia J Bedford-Guaus
    Department of Clinical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY 14853, USA
    Reprod Fertil Dev 20:875-83. 2008
  2. pmc Human sperm devoid of PLC, zeta 1 fail to induce Ca(2+) release and are unable to initiate the first step of embryo development
    Sook Young Yoon
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts, USA
    J Clin Invest 118:3671-81. 2008
  3. pmc Male infertility-linked point mutation disrupts the Ca2+ oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
    Biochem J 434:211-7. 2011
  4. doi Oolemma receptors and oocyte activation
    Kenneth L White
    Animal, Dairy and Veterinary Sciences Department, and Center for Integrated Biosystems, Utah State University, Logan, UT 84322 4815, USA
    Syst Biol Reprod Med 56:365-75. 2010
  5. doi How do spermatozoa activate oocytes?
    Brian Dale
    CFA Italia, Naples and Stazione Zoologica, Naples, Italy
    Reprod Biomed Online 21:1-3. 2010
  6. pmc NYD-SP27, a novel intrinsic decapacitation factor in sperm
    Ye Bi
    Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China
    Asian J Androl 11:229-39. 2009
  7. ncbi Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytes
    L J Cox
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
    Reproduction 124:611-23. 2002
  8. ncbi Broad, ectopic expression of the sperm protein PLCZ1 induces parthenogenesis and ovarian tumours in mice
    Naoko Yoshida
    Laboratory of Mammalian Molecular Embryology, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Development 134:3941-52. 2007
  9. ncbi Effect of NYD-SP27 down-regulation on ATP-induced Ca2+-dependent pancreatic duct anion secretion in cystic fibrosis cells
    Jin Xia Zhu
    Epithelial Cell Biology Research Center, Department of Physiology, Faculty of Medicine, Rm 410, Basic Medical Science Building, The Chinese University of Hong Kong, Shatin, Hong Kong, China
    Cell Biol Int 31:521-5. 2007
  10. ncbi Rescue of defective pancreatic secretion in cystic-fibrosis cells by suppression of a novel isoform of phospholipase C
    Hu Zhu
    Epithelial Cell Biology Research Center, Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, SAR, Shatin, Hong Kong, China
    Lancet 362:2059-65. 2003

Research Grants

  1. ELECTROSTATIC POTENTIALS AND BIOLOGICAL MEMBRANES
    Stuart McLaughlin; Fiscal Year: 2007

Scientific Experts

  • Karl Swann
  • K Coward
  • Masahiko Ito
  • Brian Dale
  • Kenneth L White
  • Junaid Kashir
  • Melissa A Cooney
  • Brigitte Ciapa
  • S J Bedford-Guaus
  • Pablo J Ross
  • Zen Kouchi
  • F A Lai
  • K T Jones
  • Shunichi Miyazaki
  • Hideki Shirakawa
  • Shamshad Cockcroft
  • Richard Ye
  • Rafael A Fissore
  • Stuart McLaughlin
  • Sook Young Yoon
  • Manabu Kurokawa
  • Michail Nomikos
  • Christopher Malcuit
  • Jason G Knott
  • Yasunari Seita
  • Mark G Larman
  • P Grasa
  • Christopher M Saunders
  • John Parrington
  • Hsiao Chang Chan
  • Naoko Yoshida
  • Jin Xia Zhu
  • Kana Sato
  • Ken ichi Sato
  • T Holden
  • Mahmoud Aarabi
  • V Kasimanickam
  • Walaa M Ramadan
  • Hu Zhu
  • Junya Ito
  • Naomi Kashiwazaki
  • S L Taylor
  • Hoi Chang Lee
  • Elke Heytens
  • R A Fissore
  • S Y Yoon
  • Young Ho Choi
  • Ye Bi
  • E Heytens
  • N T Binh
  • Shusei Mizushima
  • C Young
  • J Parrington
  • Yuansong Yu
  • Tomoko Nakanishi
  • Y Yu
  • Deivendran Rengaraj
  • Hideki Igarashi
  • Richard M Schultz
  • Keiji Kuroda
  • Bora Lee
  • Carmen J Williams
  • Kiyoko Fukami
  • Akihiro Yoneda
  • Manami Amanai
  • Katsuyuki Kinoshita
  • Anthony C F Perry
  • Hiroyuki Takeuchi
  • Tomohide Shikano
  • Tomoyuki Fukui
  • Changli He
  • Yiu Wa Chung
  • Yoshie Sone
  • C M Saunders
  • Satoko Fujimoto
  • N T Rogers
  • Lynda M Blayney
  • L J Cox
  • S Dehipawala
  • T Cheung
  • D Lieberman
  • J Ye
  • Takuya Wakai
  • G Tremberger
  • E Cheung
  • Akiko Takizawa
  • Hyung Jae Won
  • Sang Hee Park
  • Woo Sik Lee
  • Sung Han Shim

Detail Information

Publications73

  1. ncbi Microinjection of mouse phospholipase C zeta complementary RNA into mare oocytes induces long-lasting intracellular calcium oscillations and embryonic development
    Sylvia J Bedford-Guaus
    Department of Clinical Sciences, Cornell University, College of Veterinary Medicine, Ithaca, NY 14853, USA
    Reprod Fertil Dev 20:875-83. 2008
    ..Because phospholipase Czeta (PLCzeta) is the postulated sperm-borne factor responsible for oocyte activation at fertilisation, the aim of the present ..
  2. pmc Human sperm devoid of PLC, zeta 1 fail to induce Ca(2+) release and are unable to initiate the first step of embryo development
    Sook Young Yoon
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts, USA
    J Clin Invest 118:3671-81. 2008
    ..We have also shown that PLC, zeta 1 (PLCZ1), the sperm protein thought to induce [Ca(2+)](i) oscillations, was localized to the equatorial region of wild-..
  3. pmc Male infertility-linked point mutation disrupts the Ca2+ oscillation-inducing and PIP(2) hydrolysis activity of sperm PLCζ
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff CF14 4XN, UK
    Biochem J 434:211-7. 2011
    ..Wild-type PLCζ initiated a normal pattern of Ca2+ oscillations in eggs in the presence of 10-fold higher mutant PLCζ, suggesting that infertility is not mediated by a dominant-negative mechanism...
  4. doi Oolemma receptors and oocyte activation
    Kenneth L White
    Animal, Dairy and Veterinary Sciences Department, and Center for Integrated Biosystems, Utah State University, Logan, UT 84322 4815, USA
    Syst Biol Reprod Med 56:365-75. 2010
    ..This fact leads one to speculate that the process of oocyte activation by the sperm cell is a highly complex and elaborate process that likely involves many more players than perhaps was initially expected...
  5. doi How do spermatozoa activate oocytes?
    Brian Dale
    CFA Italia, Naples and Stazione Zoologica, Naples, Italy
    Reprod Biomed Online 21:1-3. 2010
    ..Questions to be answered include the identification of PLC zeta 1 in invertebrate spermatozoa and the characterization of other targets in mammalian oocytes, such as the ..
  6. pmc NYD-SP27, a novel intrinsic decapacitation factor in sperm
    Ye Bi
    Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China
    Asian J Androl 11:229-39. 2009
    ..In this study, we report that NYD-SP27, an isoform of phospholipase C Zeta 1 (PLCZ1), is localized to the sperm acrosome in mouse and human spermatozoa by immunofluorescence using a specific ..
  7. ncbi Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytes
    L J Cox
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
    Reproduction 124:611-23. 2002
    ..A sperm-specific zeta isoform of phospholipase C (PLCzeta) has been identified in mice...
  8. ncbi Broad, ectopic expression of the sperm protein PLCZ1 induces parthenogenesis and ovarian tumours in mice
    Naoko Yoshida
    Laboratory of Mammalian Molecular Embryology, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Development 134:3941-52. 2007
    ..are induced at fertilisation by a signal thought to come from the sperm protein, phospholipase C-zeta (PLCZ1)...
  9. ncbi Effect of NYD-SP27 down-regulation on ATP-induced Ca2+-dependent pancreatic duct anion secretion in cystic fibrosis cells
    Jin Xia Zhu
    Epithelial Cell Biology Research Center, Department of Physiology, Faculty of Medicine, Rm 410, Basic Medical Science Building, The Chinese University of Hong Kong, Shatin, Hong Kong, China
    Cell Biol Int 31:521-5. 2007
    ....
  10. ncbi Rescue of defective pancreatic secretion in cystic-fibrosis cells by suppression of a novel isoform of phospholipase C
    Hu Zhu
    Epithelial Cell Biology Research Center, Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, SAR, Shatin, Hong Kong, China
    Lancet 362:2059-65. 2003
    ..We aimed to identify a molecular mechanism from which a new method to circumvent defective pancreatic secretion could be derived...
  11. ncbi Preimplantation development of mouse oocytes activated by different levels of human phospholipase C zeta
    Y Yu
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Hum Reprod 23:365-73. 2008
    A sperm-specific phospholipase C zeta (PLCzeta) has been shown to trigger Ca(2+) oscillations in mouse and human oocytes and appears to be the sperm factor responsible for activation at fertilization...
  12. pmc Human immunodeficiency virus-1 glycoproteins gp120 and gp160 specifically inhibit the CD3/T cell-antigen receptor phosphoinositide transduction pathway
    D Cefai
    Laboratoire d Immunologie Cellulaire et Tissulaire, Group Hospitalier GH Pitié Salpétrière, Paris, France
    J Clin Invest 86:2117-24. 1990
    ....
  13. ncbi PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development
    Christopher M Saunders
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
    Development 129:3533-44. 2002
    ..Our results are consistent with sperm PLC zeta as the molecular trigger for development of a fertilised egg into an embryo...
  14. doi Calcium oscillations, oocyte activation, and phospholipase C zeta
    Junaid Kashir
    Nuffield Department of Obstetrics and Gynaecology, Level 3, Women s Centre, John Radcliffe Hospital, University of Oxford, England, UK
    Adv Exp Med Biol 740:1095-121. 2012
    ..now indicates that these calcium oscillations are caused by a testis-specific phospholipase C (PLC) termed PLCzeta (PLCζ), released into the oocyte following gamete fusion...
  15. doi Molecular characteristics of horse phospholipase C zeta (PLCζ)
    Kana Sato
    Laboratory of Animal Reproduction, Graduate School of Veterinary Science, Azabu University, Sagamihara 252 5201, Japan
    Anim Sci J 84:359-68. 2013
    A sperm-specific phospholipase C (PLC), PLCzeta (PLCζ), is thought to underlie the initiation of calcium ([Ca(2+) ]i ) oscillations that induce egg activation in mammals...
  16. pmc Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection
    Karl Swann
    School of Medicine, Cardiff University, Heath Park, Cardiff, United Kingdom
    Fertil Steril 97:742-7. 2012
    ..To evaluate the imaging of cytoplasmic movements in human oocytes as a potential method to monitor the pattern of Ca(2+) oscillations during activation...
  17. pmc Gene entropy-fractal dimension informatics with application to mouse-human translational medicine
    T Holden
    Queensborough Community College of CUNY, 222 05 56th Avenve Bayside, NY 11364, USA
    Biomed Res Int 2013:582358. 2013
    ..ID1 and PLCZ1 have larger separation...
  18. doi Association between mRNA abundance of functional sperm function proteins and fertility of Holstein bulls
    V Kasimanickam
    Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington, USA
    Theriogenology 78:2007-2019.e2. 2012
    ..regression models tested, a combination of AK1, IB5, TIMP2, small nuclear ribonucleoprotein polypeptide N, and PLCz1 accounted for 97.4% of the variance in SCR scores...
  19. doi Motile sperm organelle morphology evaluation-selected globozoospermic human sperm with an acrosomal bud exhibits novel patterns and higher levels of phospholipase C zeta
    Junaid Kashir
    Nuffield Department of Obstetrics and Gynaecology, Level 3, Women s Centre, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK
    Hum Reprod 27:3150-60. 2012
    ..Does motile sperm organelle morphology examination (MSOME) affect levels and localization patterns of the oocyte activation factor phospholipase C zeta (PLCζ) in globozoospermic sperm with and without an acrosomal bud?..
  20. doi Characterization of two heterozygous mutations of the oocyte activation factor phospholipase C zeta (PLCζ) from an infertile man by use of minisequencing of individual sperm and expression in somatic cells
    Junaid Kashir
    Nuffield Department of Obstetrics and Gynaecology, Women s Centre, John Radcliffe Hospital, Headington, Oxford, United Kingdom
    Fertil Steril 98:423-31. 2012
    ..To examine the underlying factors leading to infertility in a male patient from whom phospholipase C zeta H398P (PLCζ(H398P), histidine > proline) and PLCζ(H233L) (histidine > leucine) mutations were previously identified...
  21. doi Oocyte activation, phospholipase C zeta and human infertility
    J Kashir
    Nuffield Department of Obstetrics and Gynaecology, Level 3, Women s Centre, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK
    Hum Reprod Update 16:690-703. 2010
    ..How these latest findings may influence future diagnosis and treatment options are also discussed...
  22. pmc Oocyte activation and phospholipase C zeta (PLCζ): diagnostic and therapeutic implications for assisted reproductive technology
    Walaa M Ramadan
    Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Level 3, Women s Centre, John Radcliffe Hospital, Headington, Oxford, OX3, 9DU, UK
    Cell Commun Signal 10:12. 2012
    ..Recent evidence implicates a sperm-specific phospholipase C, PLCzeta (PLCζ), introduced into the oocyte following membrane fusion as the factor responsible...
  23. doi Starting a new life: sperm PLC-zeta mobilizes the Ca2+ signal that induces egg activation and embryo development: an essential phospholipase C with implications for male infertility
    Michail Nomikos
    Cell Signalling Laboratory, WHRI, Institute of Molecular and Experimental Medicine, Cardiff University School of Medicine, Cardiff, UK
    Bioessays 34:126-34. 2012
    ..Recent advances have refined our understanding of how PLCζ induces Ca(2+) oscillations in the egg and also suggest its potential dysfunction as a cause of male infertility...
  24. doi Recombinant human phospholipase C zeta 1 induces intracellular calcium oscillations and oocyte activation in mouse and human oocytes
    Sook Young Yoon
    Fertility Center of CHA Gangnam Medical Center, CHA University, Gangnam Gu, Seoul, Republic of Korea
    Hum Reprod 27:1768-80. 2012
    ..which are triggered during fertilization by the introduction of the sperm-specific phospholipase C zeta 1 (PLCZ1)...
  25. pmc The testicular and epididymal expression profile of PLCζ in mouse and human does not support its role as a sperm-borne oocyte activating factor
    Mahmoud Aarabi
    Department of Biomedical and Molecular Sciences, School of Medicine, Queen s University, Kingston, Ontario, Canada
    PLoS ONE 7:e33496. 2012
    ..Most importantly, no evidence was found that PLCζ is incorporated into the detergent-resistant perinuclear theca fraction where SOAF resides...
  26. ncbi Phospholipase Czeta causes Ca2+ oscillations and parthenogenetic activation of human oocytes
    N T Rogers
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT
    Reproduction 128:697-702. 2004
    ..We recently identified this sperm-specific protein as phospholipase Czeta (PLCzeta), and we showed that PLCzeta triggers Ca2+ oscillations in unfertilized mouse oocytes...
  27. ncbi Mammalian fertilization: from sperm factor to phospholipase Czeta
    Manabu Kurokawa
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01373, USA
    Biol Cell 96:37-45. 2004
    ..for the production of IP3 and the initiator of [Ca2+]i oscillations in mammalian fertilization, with particular emphasis on the possible involvement of egg- and sperm-derived PLCs, including PLCzeta, a novel sperm specific PLC.
  28. ncbi Calcium oscillations and mammalian egg activation
    Christopher Malcuit
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Cell Physiol 206:565-73. 2006
    ..The recent discovery that mammalian sperm harbor a specific phospholipase C (PLC), PLCzeta has consolidated this view...
  29. ncbi Regulation of fertilization-initiated [Ca2+]i oscillations in mammalian eggs: a multi-pronged approach
    Bora Lee
    Molecular and Cellular Biology Program, University of Massachusetts, Amherst, 01003, USA
    Semin Cell Dev Biol 17:274-84. 2006
    ..Hence, control of IP(3)R-1 function together with regulation of PLCzeta activity, the putative sperm factor, may combine to impart cell cycle and species-specific [Ca(2+)](i) ..
  30. ncbi Fertilization and inositol 1,4,5-trisphosphate (IP3)-induced calcium release in type-1 inositol 1,4,5-trisphosphate receptor down-regulated bovine eggs
    Christopher Malcuit
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, 01003, USA
    Biol Reprod 73:2-13. 2005
    ..of total intact receptor to mount persistent [Ca(2+)](i) oscillations in response to fertilization, expression of PLCzeta (also known as PLCZ1), and adenophostin A...
  31. ncbi Teleost fish spermatozoa contain a cytosolic protein factor that induces calcium release in sea urchin egg homogenates and triggers calcium oscillations when injected into mouse oocytes
    Kevin Coward
    Department of Pharmacology, University of Oxford, Oxford OX1 3QT, UK
    Biochem Biophys Res Commun 305:299-304. 2003
    ..identified this sperm-derived factor as being a novel sperm-specific PLC isoform with distinctive properties (PLCzeta). Homologues of PLCzeta have since been isolated from human and cynomolgus monkey sperm...
  32. doi Reduced fertilization after ICSI and abnormal phospholipase C zeta presence in spermatozoa from the wobbler mouse
    Elke Heytens
    Department of Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, B 9000 Ghent, Belgium
    Reprod Biomed Online 21:742-9. 2010
    ..The presence of PLCzeta in the spermatozoa and testis of the wobbler mouse was evaluated by PLCzeta immunostaining and quantitative ..
  33. ncbi Role of phospholipase C-zeta domains in Ca2+-dependent phosphatidylinositol 4,5-bisphosphate hydrolysis and cytoplasmic Ca2+ oscillations
    Michail Nomikos
    Cell Signalling Laboratory, Wales Heart Research Institute, School of Medicine, Cardiff University, Cardiff CF14 4XN
    J Biol Chem 280:31011-8. 2005
    The sperm-specific phospholipase C-zeta (PLCzeta) elicits fertilization-like Ca2+ oscillations and activation of embryo development when microinjected into mammalian eggs (Saunders, C. M., Larman, M. G., Parrington, J., Cox, L. J...
  34. ncbi Phospholipase Czeta, the trigger of egg activation in mammals, is present in a non-mammalian species
    K Coward
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
    Reproduction 130:157-63. 2005
    ..but recent studies suggest that, in mammals, egg activation is triggered by a testis-specific phospholipase C: PLCzeta. Since the discovery of PLCzeta, it has been unclear whether its role in triggering egg activation is common to ..
  35. ncbi Functional, biochemical, and chromatographic characterization of the complete [Ca2+]i oscillation-inducing activity of porcine sperm
    Manabu Kurokawa
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA
    Dev Biol 285:376-92. 2005
    ..Recent evidence shows that the novel sperm-specific phospholipase C (PLC), PLCzeta, may be the long sought after [Ca2+]i oscillation-inducing SF...
  36. pmc Species-specific differences in the activity and nuclear localization of murine and bovine phospholipase C zeta 1
    Melissa A Cooney
    Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia
    Biol Reprod 83:92-101. 2010
    Injection of mammalian sperm extracts or cRNA of the sperm-specific phospholipase C zeta 1 (PLCZ1) has been shown to trigger repetitive oscillations in the concentration of free calcium ([Ca(2+)](i)), leading to oocyte activation and ..
  37. pmc Complete globozoospermia associated with PLCζ deficiency treated with calcium ionophore and ICSI results in pregnancy
    S L Taylor
    The Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, VA, USA
    Reprod Biomed Online 20:559-64. 2010
    ..transmission electron microscopy, Western blotting and immunofluorescence for detection of phospholipase C zeta (PLCzeta), as well as ICSI outcome, of an affected patient...
  38. doi Phospholipase Czeta mRNA expression and its potency during spermatogenesis for activation of quail oocyte as a sperm factor
    Shusei Mizushima
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, Japan
    Mol Reprod Dev 76:1200-7. 2009
    ..First, effects of a microinjection of quail sperm extract (SE) and quail phospholipase Czeta (PLCzeta) cRNA into quail oocytes were assessed by observation of pronuclear formation and cytoplasmic segmentation, ..
  39. doi Reduced amounts and abnormal forms of phospholipase C zeta (PLCzeta) in spermatozoa from infertile men
    E Heytens
    Department of Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium
    Hum Reprod 24:2417-28. 2009
    ..In mammals, oocyte activation at fertilization is thought to be induced by the sperm-specific phospholipase C zeta (PLCzeta). However, it still remains to be conclusively shown that PLCzeta is the endogenous agent of oocyte activation...
  40. ncbi Nuclear translocation of phospholipase C-zeta, an egg-activating factor, during early embryonic development
    Yoshie Sone
    Department of Physiology, Tokyo Women s Medical University School of Medicine, Tokyo 162 8666, Japan
    Biochem Biophys Res Commun 330:690-4. 2005
    Phospholipase C-zeta (PLCzeta), a strong candidate of the egg-activating sperm factor, causes intracellular Ca2+ oscillations and egg activation, and is subsequently accumulated into the pronucleus (PN), when expressed in mouse eggs by ..
  41. doi Identification and functional analysis of an ovarian form of the egg activation factor phospholipase C zeta (PLCζ) in pufferfish
    Kevin Coward
    Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, Oxford, UK
    Mol Reprod Dev 78:48-56. 2011
    Recent studies suggest that egg activation in mammals is triggered by a sperm-specific phospholipase C, PLCzeta. In other vertebrate species such as medaka fish, chickens, and quail, PLCzeta is also expressed as a testis-specific mRNA...
  42. ncbi Recombinant phospholipase Czeta has high Ca2+ sensitivity and induces Ca2+ oscillations in mouse eggs
    Zen Kouchi
    Department of Physiology, Tokyo Women s Medical University School of Medicine, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    J Biol Chem 279:10408-12. 2004
    Sperm-specific phospholipase Czeta (PLCzeta) is known to induce intracellular Ca(2+) oscillations and subsequent early embryonic development when expressed in mouse eggs by injection of RNA encoding PLCzeta (Saunders, C. M., Larman, M. G...
  43. ncbi The cytosolic sperm factor that triggers Ca2+ oscillations and egg activation in mammals is a novel phospholipase C: PLCzeta
    K Swann
    Department of Anatomy and Developmental Biology, University College London, London WC1E 6BT, UK
    Reproduction 127:431-9. 2004
    ..shown that this soluble sperm factor protein is a novel form of phospholipase C (PLC), and it is referred to as PLCzeta(zeta)...
  44. ncbi Cell cycle-dependent Ca2+ oscillations in mouse embryos are regulated by nuclear targeting of PLCzeta
    Mark G Larman
    Department of Anatomy and Developmental Biology, University College London, WC1E 6BT, UK
    J Cell Sci 117:2513-21. 2004
    ..We recently identified a novel, sperm-specific phospholipase C (PLCzeta; PLCzeta) that triggers Ca2+ oscillations similar to those caused by sperm...
  45. doi Could modifications of signalling pathways activated after ICSI induce a potential risk of epigenetic defects?
    Brigitte Ciapa
    UMR CNRS 8195 Centre de Neurosciences Paris Sud CNPS, Universite Paris XI, Orsay Cedex, France
    Int J Dev Biol 55:143-52. 2011
    ..Among the many potential sperm factors, PLCzeta is the strongest bona fide sperm factor candidate...
  46. ncbi Transgenic RNA interference reveals role for mouse sperm phospholipase Czeta in triggering Ca2+ oscillations during fertilization
    Jason G Knott
    Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biol Reprod 72:992-6. 2005
    A sperm-specific phospholipase (PL) C, termed PLCzeta, is proposed to be the soluble sperm factor that induces Ca(2+) oscillations in mammalian eggs and, thus, initiates egg activation in vivo...
  47. doi Difference in Ca2+ oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, between mouse, rat, human, and medaka fish
    Masahiko Ito
    Department of Physiology, Tokyo Women s Medical University School of Medicine, Shinjuku ku, Tokyo 162 8666, Japan
    Biol Reprod 78:1081-90. 2008
    Mouse phospholipase C, zeta 1 (PLCZ1), a strong candidate of egg-activating sperm factor, induces Ca(2+) oscillations and accumulates into formed pronucleus (PN) when expressed by cRNA injection...
  48. pmc A maternally inherited autosomal point mutation in human phospholipase C zeta (PLCζ) leads to male infertility
    Junaid Kashir
    Nuffield Department of Obstetrics and Gynaecology, Level 3, Women s Centre, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK
    Hum Reprod 27:222-31. 2012
    ..a process normally regulated by calcium oscillations, thought to be induced by a sperm-specific phospholipase C, PLCzeta (PLCζ)...
  49. ncbi The role of EF-hand domains and C2 domain in regulation of enzymatic activity of phospholipase Czeta
    Zen Kouchi
    Department of Physiology, Tokyo Women s Medical University School of Medicine, Shinjuku ku, Tokyo 162 8666, Japan
    J Biol Chem 280:21015-21. 2005
    Sperm-specific phospholipase C-zeta (PLCzeta) induces Ca2+ oscillations and egg activation when injected into mouse eggs...
  50. doi Effect of sperm extract injection volume, injection of PLCzeta cRNA, and tissue cell line on efficiency of equine nuclear transfer
    Young Ho Choi
    College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843 4466, USA
    Cloning Stem Cells 11:301-8. 2009
    ..2 sec (0%) or 0.8 sec (1.4%). In Experiment 2, injection of murine PLCzeta cRNA before or after ionomycin treatment did not increase blastocyst development (0 and 4...
  51. ncbi Removal of acrosomal membrane from sperm head improves development of rat zygotes derived from intracytoplasmic sperm injection
    Yasunari Seita
    Laboratory of Animal Reproduction, Graduate School of Veterinary Science, Azabu University, Sagamihira, Japan
    J Reprod Dev 55:475-9. 2009
    ..05). However, neither TX nor LL treatment affected amounts of PLCzeta in rat sperm. The rates of offspring derived from TX- (20.3 +/- 4.4%) and LL-treated sperm (19.0 +/- 2...
  52. pmc Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta
    Pablo J Ross
    Cellular Reprogramming Laboratory, Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA
    BMC Dev Biol 8:16. 2008
    ..b>PLCZ1 has been identified as the factor that the sperm delivers into the egg to induce such a response...
  53. pmc Proteolytic processing of phospholipase Czeta and [Ca2+]i oscillations during mammalian fertilization
    Manabu Kurokawa
    Paige Laboratory, Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA
    Dev Biol 312:407-18. 2007
    Phospholipase Czeta (PLCzeta) is a sperm-specific PLC capable of causing repetitive intracellular Ca2+ ([Ca2+]i) release ([Ca2+]i oscillations) in mammalian eggs...
  54. ncbi Release of phospholipase C zetaand [Ca2+]i oscillation-inducing activity during mammalian fertilization
    Sook Young Yoon
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts 01003, USA
    Reproduction 134:695-704. 2007
    ..We also stained the enucleated sperm heads to ascertain the presence/absence of PLCzeta. Our results show that by 90 min all the SF activity had been released from the sperm, as fertilized enucleated ..
  55. pmc Alterations of PLCbeta1 in mouse eggs change calcium oscillatory behavior following fertilization
    Hideki Igarashi
    Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia, PA 19104, USA
    Dev Biol 312:321-30. 2007
    ..Much attention currently focuses on the role of sperm-derived PLCzeta in generating changes in egg intracellular Ca2+ despite the fact that PLCzeta constitutes a very small fraction ..
  56. ncbi Expression of a fluorescent recombinant form of sperm protein phospholipase C zeta in mouse epididymal sperm by in vivo gene transfer into the testis
    Kevin Coward
    Department of Pharmacology, University of Oxford, Oxford, United Kingdom
    Fertil Steril 85:1281-9. 2006
    ..To use in vivo gene transfer into the testis by electroporation to express a fluorescent recombinant form of a testis-specific gene in the mature epididymal sperm of mice and thus study the pattern of gene localization...
  57. ncbi Intracellular calcium in the fertilization and development of mammalian eggs
    Keith T Jones
    Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, UK
    Clin Exp Pharmacol Physiol 34:1084-9. 2007
    ..3. Ca(2+) spiking is also observed in mitosis of one-cell embryos, probably because PLCzeta contains a nuclear localization signal and so is released into the cytoplasm following nuclear envelope breakdown...
  58. ncbi Measurement of intracellular IP3 during Ca2+ oscillations in mouse eggs with GFP-based FRET probe
    Hideki Shirakawa
    Department of Applied Physics and Chemistry, The University of Electro Communications, Tokyo 182 8585, Japan
    Biochem Biophys Res Commun 345:781-8. 2006
    ..protein variants, during Ca2+ oscillations induced by fertilization or expression of phospholipase Czeta (PLCzeta), an egg-activating sperm factor candidate...
  59. ncbi Molecular cloning, testicular postnatal expression, and oocyte-activating potential of porcine phospholipase Czeta
    Akihiro Yoneda
    Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo 060 8589, Japan
    Reproduction 132:393-401. 2006
    ..Recently, oocyte activation has been shown to be induced by sperm-specific phospholipase Czeta (PLCzeta). The ability of PLCzeta to induce oocyte activation is highly conserved across vertebrates...
  60. ncbi The role of X/Y linker region and N-terminal EF-hand domain in nuclear translocation and Ca2+ oscillation-inducing activities of phospholipase Czeta, a mammalian egg-activating factor
    Keiji Kuroda
    Department of Physiology, Tokyo Women s Medical University School of Medicine, Tokyo 162 8666, Japan
    J Biol Chem 281:27794-805. 2006
    Sperm-specific phospholipase C-zeta (PLCzeta) causes intracellular Ca(2+) oscillations and thereby egg activation and is accumulated into the formed pronucleus (PN) when expressed in mouse eggs by injection of cRNA encoding PLCzeta, ..
  61. ncbi Calcium signals for egg activation in mammals
    Shunichi Miyazaki
    Department of Physiology, Tokyo Women s Medical University School of Medicine, Japan
    J Pharmacol Sci 100:545-52. 2006
    ..candidate of the sperm factor is a novel sperm-specific isozyme of phospholipase C (IP(3)-producing enzyme), PLCzeta. Recent extensive research has reveled characteristics of PLCzeta such as the Ca(2+) oscillation-inducing ..
  62. ncbi Mammalian egg activation: from Ca2+ spiking to cell cycle progression
    Keith T Jones
    Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
    Reproduction 130:813-23. 2005
    ..pathway remain to be fully investigated, here I present a model in which a sperm-specific phospholipase C (PLCzeta) generates Ca(2+) spikes to activate calmodulin-dependent protein kinase II and so switch on the Anaphase-..
  63. doi Testis-specific novel transcripts in chicken: in situ localization and expression pattern profiling during sexual development
    Deivendran Rengaraj
    Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea
    Biol Reprod 79:413-20. 2008
    ..Among the testis-specific TC sequences, TC120901 is a known gene, phospholipase C, zeta (PLCZ1)...
  64. doi Effects of liquid preservation of sperm on their ability to activate oocytes and initiate preimplantational development after intracytoplasmic sperm injection in the pig
    N T Binh
    Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    Theriogenology 71:1440-50. 2009
    ..then damage to the plasma membrane, morphologic changes of the acrosome, and the amount of phospholipase Czeta (PLCzeta) in the sperm were assessed by SYBR-14/propidium iodide staining, fluorescein isothiocyanate-conjugated peanut ..
  65. ncbi Mammalian phospholipase Czeta induces oocyte activation from the sperm perinuclear matrix
    Satoko Fujimoto
    Laboratory of Mammalian Molecular Embryology, RIKEN Center for Developmental Biology, Chuo Ku, Kobe 650 0047, Japan
    Dev Biol 274:370-83. 2004
    ..Phospholipase C-zeta (PLCzeta) correlated absolutely with activating ability...
  66. ncbi Relationship between nuclear sequestration of PLCzeta and termination of PLCzeta-induced Ca2+ oscillations in mouse eggs
    Masahiko Ito
    Department of Physiology, Tokyo Women s Medical University School of Medicine, Shinjuku ku, Tokyo 162 8666, Japan
    Cell Calcium 44:400-10. 2008
    Phospholipase C-zeta (PLCzeta), a strong candidate of the egg-activating sperm factor, causes long-lasting series of Ca2+ spikes and thereby egg activation when expressed in mouse eggs by injection of cRNA...
  67. doi Use of luciferase chimaera to monitor PLCzeta expression in mouse eggs
    Karl Swann
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
    Methods Mol Biol 518:17-29. 2009
    ..The PLCzeta protein that is expressed in eggs after injection of cRNA is effective in causing Ca2+ oscillations at very low ..
  68. ncbi The latest phospholipase C, PLCeta, is implicated in neuronal function
    Shamshad Cockcroft
    Department of Physiology, University College London, London WC1E 6JJ, UK
    Trends Biochem Sci 31:4-7. 2006
    ..into five families designated PLCbeta (1-4), PLCgamma (1 and 2), PLCdelta (1, 3 and 4), PLCepsilon (1) and PLCzeta (1)...
  69. doi The pattern of localization of the putative oocyte activation factor, phospholipase Czeta, in uncapacitated, capacitated, and ionophore-treated human spermatozoa
    P Grasa
    Department of Pharmacology, University of Oxford, Oxford, UK
    Hum Reprod 23:2513-22. 2008
    Recent studies suggest that in mammals, oocyte activation at fertilization is triggered by a sperm-specific phospholipase C, PLCzeta. We investigated PLCzeta localization in human spermatozoa.
  70. doi The dynamics of calcium oscillations that activate mammalian eggs
    Karl Swann
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
    Int J Dev Biol 52:585-94. 2008
    ..b>PLCzeta is stimulated by low intracellular calcium levels in a manner which suggests that there is a regenerative ..
  71. ncbi Birth of normal offspring from mouse eggs activated by a phospholipase Czeta protein lacking three EF-hand domains
    Tomoko Nakanishi
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
    J Reprod Dev 54:244-9. 2008
    Sperm-specific phospholipase C, PLCzeta, is a candidate for the Ca(2+) oscillation-inducing factor that is introduced into the ooplasm upon sperm-egg fusion...
  72. pmc Regulation of diacylglycerol production and protein kinase C stimulation during sperm- and PLCzeta-mediated mouse egg activation
    Yuansong Yu
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, U K
    Biol Cell 100:633-43. 2008
    ..Increased inositol 1,4,5-trisphosphate production appears to be triggered by a sperm-derived PLCzeta (phospholipase C-zeta) that enters the egg after gamete fusion...
  73. ncbi PLCzeta(zeta): a sperm protein that triggers Ca2+ oscillations and egg activation in mammals
    K Swann
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Semin Cell Dev Biol 17:264-73. 2006
    ..We recently identified a sperm-specific form of phospholipase C (PLC), referred to as PLCzeta(zeta)...

Research Grants5

  1. ELECTROSTATIC POTENTIALS AND BIOLOGICAL MEMBRANES
    Stuart McLaughlin; Fiscal Year: 2007
    ..unstructured basic clusters provide sufficient specificity for PIP2 to target proteins such as the sperm factor PLCzeta to the plasma membrane...