PKN1

Summary

Gene Symbol: PKN1
Description: protein kinase N1
Alias: DBK, PAK-1, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1, protease-activated kinase 1, protein kinase C-like 1, protein kinase C-like PKN, protein kinase C-related kinase 1, protein kinase PKN-alpha, protein-kinase C-related kinase 1, serine-threonine kinase N, serine-threonine protein kinase N, serine/threonine protein kinase N
Species: human

Top Publications

  1. pmc A-kinase anchoring protein (AKAP)-Lbc anchors a PKN-based signaling complex involved in α1-adrenergic receptor-induced p38 activation
    Luca Cariolato
    Département de Pharmacologie et de Toxicologie, Faculté de Biologie et Médecine, University of Lausanne, Lausanne 1005, Switzerland
    J Biol Chem 286:7925-37. 2011
  2. pmc Identification of an interaction between the TPalpha and TPbeta isoforms of the human thromboxane A2 receptor with protein kinase C-related kinase (PRK) 1: implications for prostate cancer
    Elizebeth C Turner
    School of Biomolecular and Biomedical Sciences, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
    J Biol Chem 286:15440-57. 2011
  3. ncbi The structural basis of Rho effector recognition revealed by the crystal structure of human RhoA complexed with the effector domain of PKN/PRK1
    R Maesaki
    Division of Structural Biology, Nara Institute of Science and Technology, Japan
    Mol Cell 4:793-803. 1999
  4. pmc A novel inducible transactivation domain in the androgen receptor: implications for PRK in prostate cancer
    Eric Metzger
    Universitäts Frauenklinik und Zentrum für Klinische Forschung, Klinikum der Universität Freiburg, Breisacherstrasse 66, D 79106 Freiburg, Germany
    EMBO J 22:270-80. 2003
  5. pmc Phosphorylation of protein kinase N by phosphoinositide-dependent protein kinase-1 mediates insulin signals to the actin cytoskeleton
    L Q Dong
    Department of Pharmacology and Biochemistry, The University of Texas Health Science Center, San Antonio, TX 78229, USA
    Proc Natl Acad Sci U S A 97:5089-94. 2000
  6. ncbi Interaction of PKN with alpha-actinin
    H Mukai
    Radioisotope Research Center, Kobe University, Kobe 657, Japan
    J Biol Chem 272:4740-6. 1997
  7. pmc Proteolytic activation of PKN by caspase-3 or related protease during apoptosis
    M Takahashi
    Department of Biology, Faculty of Science, Kobe University, Kobe 657 8501, Japan
    Proc Natl Acad Sci U S A 95:11566-71. 1998
  8. doi Negative regulation of constitutive NF-kappaB and JNK signaling by PKN1-mediated phosphorylation of TRAF1
    Tomohisa Kato
    Biosignal Research Center, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Genes Cells 13:509-20. 2008
  9. ncbi PKNbeta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family
    H Shibata
    Department of Biology, Faculty of Science, Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    J Biochem 130:23-31. 2001
  10. ncbi Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family
    R H Palmer
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, England
    Eur J Biochem 227:344-51. 1995

Detail Information

Publications203 found, 100 shown here

  1. pmc A-kinase anchoring protein (AKAP)-Lbc anchors a PKN-based signaling complex involved in α1-adrenergic receptor-induced p38 activation
    Luca Cariolato
    Département de Pharmacologie et de Toxicologie, Faculté de Biologie et Médecine, University of Lausanne, Lausanne 1005, Switzerland
    J Biol Chem 286:7925-37. 2011
    ..Our results indicate that AKAP-Lbc can assemble a novel transduction complex containing the RhoA effector PKNα, MLTK, MKK3, and p38α, which integrates signals from α(1b)-ARs to promote RhoA-dependent activation of p38α...
  2. pmc Identification of an interaction between the TPalpha and TPbeta isoforms of the human thromboxane A2 receptor with protein kinase C-related kinase (PRK) 1: implications for prostate cancer
    Elizebeth C Turner
    School of Biomolecular and Biomedical Sciences, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
    J Biol Chem 286:15440-57. 2011
    ..involving common and unique sequences within their respective C-terminal (C)-tail domains and the kinase domain of PRK1 (PRK1(640-942))...
  3. ncbi The structural basis of Rho effector recognition revealed by the crystal structure of human RhoA complexed with the effector domain of PKN/PRK1
    R Maesaki
    Division of Structural Biology, Nara Institute of Science and Technology, Japan
    Mol Cell 4:793-803. 1999
    ..Here we report the 2.2 A crystal structure of RhoA bound to an effector domain of protein kinase PKN/PRK1. The structure reveals the antiparallel coiled-coil finger (ACC finger) fold of the effector domain that binds to ..
  4. pmc A novel inducible transactivation domain in the androgen receptor: implications for PRK in prostate cancer
    Eric Metzger
    Universitäts Frauenklinik und Zentrum für Klinische Forschung, Klinikum der Universität Freiburg, Breisacherstrasse 66, D 79106 Freiburg, Germany
    EMBO J 22:270-80. 2003
    ..Here, we unravel a novel signal transduction pathway that links the RhoA effector protein kinase C-related kinase PRK1 to the transcriptional activation of the androgen receptor (AR)...
  5. pmc Phosphorylation of protein kinase N by phosphoinositide-dependent protein kinase-1 mediates insulin signals to the actin cytoskeleton
    L Q Dong
    Department of Pharmacology and Biochemistry, The University of Texas Health Science Center, San Antonio, TX 78229, USA
    Proc Natl Acad Sci U S A 97:5089-94. 2000
    ..3-kinase downstream effector phosphoinositide-dependent protein kinase-1 (PDK1) interacted with protein kinase N (PKN), a Rho-binding Ser/Thr protein kinase potentially implicated in a variety of cellular events, including ..
  6. ncbi Interaction of PKN with alpha-actinin
    H Mukai
    Radioisotope Research Center, Kobe University, Kobe 657, Japan
    J Biol Chem 272:4740-6. 1997
    b>PKN is a fatty acid- and Rho-activated serine/threonine protein kinase, having a catalytic domain homologous to protein kinase C family...
  7. pmc Proteolytic activation of PKN by caspase-3 or related protease during apoptosis
    M Takahashi
    Department of Biology, Faculty of Science, Kobe University, Kobe 657 8501, Japan
    Proc Natl Acad Sci U S A 95:11566-71. 1998
    b>PKN, a fatty acid- and Rho-activated serine/threonine kinase having a catalytic domain highly homologous to protein kinase C (PKC), was cleaved at specific sites in apoptotic Jurkat and U937 cells on Fas ligation and treatment with ..
  8. doi Negative regulation of constitutive NF-kappaB and JNK signaling by PKN1-mediated phosphorylation of TRAF1
    Tomohisa Kato
    Biosignal Research Center, Kobe University, 1 1 Rokkodai cho, Nada Ku, Kobe 657 8501, Japan
    Genes Cells 13:509-20. 2008
    ..Here, we demonstrate that the serine/threonine kinase PKN1 plays a critical role in regulating constitutive IKK/JNK activity in unstimulated cells and report on the molecular ..
  9. ncbi PKNbeta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family
    H Shibata
    Department of Biology, Faculty of Science, Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    J Biochem 130:23-31. 2001
    ..The catalytically active form of PKNbeta phosphorylated Graf and Graf2 in vitro. The interplay of PKNbeta and the GTPase-activating proteins, Graf and Graf2, may offer a novel mechanism regulating the Rho-mediated signaling...
  10. ncbi Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family
    R H Palmer
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, England
    Eur J Biochem 227:344-51. 1995
    ..These protein-kinase-C-related kinases, PRK1 and PRK2, display extensive identity to each other, revealing non-kinase domain similar regions. HR1 and HR2...
  11. ncbi Up-regulation of myometrial RHO effector proteins (PKN1 and DIAPH1) and CPI-17 (PPP1R14A) phosphorylation in human pregnancy is associated with increased GTP-RHOA in spontaneous preterm labor
    J Lartey
    Clinical Sciences at South Bristol, Division of Obstetrics and Gynaecology, Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, Dorothy Hodgkin Building, University of Bristol, Bristol BS1 3NY, United Kingdom
    Biol Reprod 76:971-82. 2007
    ..The RHO effector protein kinase N (PKN1) also increases calcium sensitization by phosphorylating the protein kinase C (PRKCB)-dependent protein CPI-17 (..
  12. ncbi PRK1 is targeted to endosomes by the small GTPase, RhoB
    H Mellor
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 273:4811-4. 1998
    ..We show here that the recently isolated serine/threonine kinase PRK1 is targeted to the endosomal compartment by RhoB...
  13. ncbi Molecular dissection of the interaction between the small G proteins Rac1 and RhoA and protein kinase C-related kinase 1 (PRK1)
    Darerca Owen
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, United Kingdom
    J Biol Chem 278:50578-87. 2003
    b>PRK1 is a serine/threonine kinase that belongs to the protein kinase C superfamily. It can be activated either by members of the Rho family of small G proteins, by proteolysis, or by interaction with lipids...
  14. ncbi Regulation of a mitogen-activated protein kinase kinase kinase, MLTK by PKN
    Mikiko Takahashi
    Biosignal Research Center and Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    J Biochem 133:181-7. 2003
    ..These results suggest that PKNalpha functions as not only an upstream activator of MLTKalpha but also a putative scaffold protein for the p38gamma MAPK signaling pathway...
  15. pmc Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation
    Eric Metzger
    Universitäts Frauenklinik und Zentrale Klinische Forschung, Klinikum der Universität Freiburg, Breisacherstrasse 66, 79106 Freiburg, Germany
    Nat Cell Biol 10:53-60. 2008
    ..Here we show that protein-kinase-C-related kinase 1 (PRK1) phosphorylates histone H3 at threonine 11 (H3T11) upon ligand-dependent recruitment to androgen receptor target ..
  16. ncbi Further evidence that 3-phosphoinositide-dependent protein kinase-1 (PDK1) is required for the stability and phosphorylation of protein kinase C (PKC) isoforms
    A Balendran
    MRC Protein Phosphorylation, MSI WTB complex, University of Dundee, Dow Street, DD1 5EH, Dundee, UK
    FEBS Lett 484:217-23. 2000
    ..levels of endogenously expressed PKCalpha, PKCbetaI, PKCgamma, PKCdelta, PKCepsilon, and PKC-related kinase-1 (PRK1) are vastly reduced compared to control ES cells (PDK1+/+ cells)...
  17. ncbi Multiple interactions of PRK1 with RhoA. Functional assignment of the Hr1 repeat motif
    P Flynn
    Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 273:2698-705. 1998
    b>PRK1 (PKN) is a serine/threonine kinase that has been shown to be activated by RhoA (Amano, M., Mukai, H., Ono, Y., Chihara, K., Matsui, T., Hamajima, Y., Okawa, K., Iwamatsu, A., and Kaibuchi, K. (1996) Science 271, 648-650)...
  18. ncbi A 3-phosphoinositide-dependent protein kinase-1 (PDK1) docking site is required for the phosphorylation of protein kinase Czeta (PKCzeta ) and PKC-related kinase 2 by PDK1
    A Balendran
    MRC Protein Phosphorylation Unit, Division of Signal Transduction Therapy, MSI WTB complex, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom
    J Biol Chem 275:20806-13. 2000
    ..In the present study we demonstrate that the hydrophobic motifs of PKCzeta and PKCiota, as well as PRK1 and PRK2, interact with the kinase domain of PDK1...
  19. ncbi Domain-specific phosphorylation of vimentin and glial fibrillary acidic protein by PKN
    K Matsuzawa
    Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Japan
    Biochem Biophys Res Commun 234:621-5. 1997
    b>PKN is a serine/threonine protein kinase with a catalytic domain homologous to the protein kinase C family and unique N-terminal leucine zipper-like sequences...
  20. doi Deregulation of PKN1 activity disrupts neurofilament organisation and axonal transport
    Catherine Manser
    MRC Centre for Neurodegeneration Research, Department of Neuroscience P037, Institute of Psychiatry, King s College, De Crespigny Park, Denmark Hill, London, United Kingdom
    FEBS Lett 582:2303-8. 2008
    ..Damage to neurofilament transport is seen in amyotrophic lateral sclerosis (ALS). Here, we show that PKN1, a neurofilament head-rod domain kinase is cleaved and activated in SOD1G93A transgenic mice that are a model of ..
  21. ncbi PKN associates and phosphorylates the head-rod domain of neurofilament protein
    H Mukai
    Department of Biology, Faculty of Science, Kobe University, Japan
    J Biol Chem 271:9816-22. 1996
    b>PKN is a fatty acid-activated serine/threonine kinase that has a catalytic domain highly homologous to that of protein kinase C in the carboxyl terminus and a unique regulatory region in the amino terminus...
  22. ncbi Protein kinase PKN1 associates with TRAF2 and is involved in TRAF2-NF-kappaB signaling pathway
    Yusuke Gotoh
    Graduate School of Science and Technology, Kobe University, 657 8501, Kobe, Japan
    Biochem Biophys Res Commun 314:688-94. 2004
    b>PKN1 is a fatty acid and Rho-activated serine/threonine protein kinase whose catalytic domain is highly homologous to protein kinase C (PKC) family...
  23. doi Postmortem photonic imaging of lux-modified Salmonella Typhimurium within the gastrointestinal tract of swine after oral inoculation in vivo
    K Moulton
    Department of Animal and Dairy Sciences, Mississippi State University, MS 39762, USA
    J Anim Sci 87:2239-44. 2009
    ..1 or 4.1 x 10(10) cfu of Salmonella Typhimurium transformed with plasmid pAK1-lux for a 6-h (n = 6) or 12-h (n = 6) incubation in vivo and then were killed for tissue harvest...
  24. pmc A role for the retinoblastoma protein as a regulator of mouse osteoblast cell adhesion: implications for osteogenesis and osteosarcoma formation
    Bernadette Sosa-García
    Biochemistry Department, Ponce School of Medicine, Ponce, Puerto Rico
    PLoS ONE 5:e13954. 2010
    ..the function of merlin, a well-known regulator of adherens junction assembly, by repressing Rac1 and its effector Pak1. Using qRT-PCR, immunoblots, co-immunoprecipitation assays, and immunofluorescent labeling, we observed that pRb ..
  25. pmc Differential signalling by muscarinic receptors in smooth muscle: m2-mediated inactivation of myosin light chain kinase via Gi3, Cdc42/Rac1 and p21-activated kinase 1 pathway, and m3-mediated MLC20 (20 kDa regulatory light chain of myosin II) phosphorylat
    Karnam S Murthy
    Departments of Physiology and Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA
    Biochem J 374:145-55. 2003
    ..phosphoinositide 3-kinase and Cdc42/Rac1 (where Cdc stands for cell division cycle) and p21-activated kinase 1 (PAK1), resulting in phosphorylation and inactivation of myosin light chain kinase (MLCK)...
  26. doi Comparison of azaperone-detomidine-butorphanol-ketamine and azaperone-tiletamine-zolazepam for anaesthesia in piglets
    Mari L Heinonen
    Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Finland
    Vet Anaesth Analg 36:151-7. 2009
    To investigate a combination of azaperone, detomidine, butorphanol and ketamine (DBK) in pigs and to compare it with the combination of azaperone, tiletamine and zolazepam (TZ).
  27. ncbi The small GTP-binding protein rho activates c-Jun N-terminal kinases/stress-activated protein kinases in human kidney 293T cells. Evidence for a Pak-independent signaling pathway
    H Teramoto
    Laboratory of Cellular Development and Oncology, NIDR, National Institutes of Health, Bethesda, Maryland 20892 4330, USA
    J Biol Chem 271:25731-4. 1996
    ..Furthermore, because Rac1 and Cdc42 but not Rho bind and activate a kinase known as Pak1, it has been suggested that Pak1 is the most upstream component of the pathway linking these GTPases to JNK...
  28. doi CAPE (caffeic acid phenethyl ester)-based propolis extract (Bio 30) suppresses the growth of human neurofibromatosis (NF) tumor xenografts in mice
    M Demestre
    UKE Universitaets Klinikum Eppendorf, Hamburg 20246, Germany
    Phytother Res 23:226-30. 2009
    ..type 2 (NF2) is caused primarily by dysfunction of the NF2 gene product called merlin that inhibits directly PAK1, an oncogenic Rac/CDC42-dependent Ser/Thr kinase...
  29. pmc Association of a purine-analogue-sensitive protein kinase activity with p75 nerve growth factor receptors
    C Volonte
    Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Mol Biol Cell 4:71-8. 1993
    ..The analogue 6-thioguanine (6-TG) has been shown to inhibit with high specificity protein kinase N (PKN), a serine/threonine protein kinase activated by NGF in several cellular systems...
  30. pmc Regulation of the actin cytoskeleton organization in yeast by a novel serine/threonine kinase Prk1p
    G Zeng
    Institute of Molecular and Cell Biology, National University of Singapore, Singapore 117609
    J Cell Biol 144:71-82. 1999
    ..The suppression of pan1-4 by prk1 requires the presence of mutant Pan1p...
  31. pmc PAK1, a gene that can regulate p53 activity in yeast
    S Thiagalingam
    Johns Hopkins Oncology Center, Johns Hopkins School of Medicine, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 92:6062-6. 1995
    ..The defect was partially corrected by overexpression of a yeast gene named PAK1 (p53 activating kinase), which localizes to the left arm of chromosome IX...
  32. pmc Pharmacological and functional characterization of the guinea-pig B2 bradykinin receptor stably expressed in CHO-K1 cell line
    C Robert
    Groupe de Pharmacologie des Récepteurs, Centre de Recherche, Laboratoires Fournier, 50 Rue de Dijon, 21121 Daix, France
    Br J Pharmacol 135:462-8. 2002
    ..21+/-0.05 nM while the two specific kinin B(1) ligands, des-Arg(9)-bradykinin (DBK) and des-Arg(9)-Leu(8)-bradykinin (DLBK) were unable to compete with [(3)H]-BK...
  33. pmc Clathrin-independent endocytosis used by the IL-2 receptor is regulated by Rac1, Pak1 and Pak2
    Alexandre Grassart
    Institut Pasteur, Unite de Biologie des Interactions Cellulaires, CNRS URA 2582, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    EMBO Rep 9:356-62. 2008
    ..By RNA interference, we show that Pak1 and Pak2 are both necessary for IL-2R beta uptake, in contrast to the clathrin-dependent route...
  34. pmc p21-Activated kinase-1 promotes aggressive phenotype, cell proliferation, and invasion in gestational trophoblastic disease
    Michelle K Y Siu
    Department of Pathology, The University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Hong Kong, China
    Am J Pathol 176:3015-22. 2010
    ..p21-Activated kinases (PAKs) promote malignant tumor progression. Therefore, this study investigated PAK1, PAK2, and p-PAK2 Ser(20) in the pathogenesis of GTD...
  35. pmc Photonic plasmid stability of transformed Salmonella typhimurium: a comparison of three unique plasmids
    Keesla Moulton
    Department of Animal and Dairy Science, Mississippi State University, Mississippi State, MS 39762, USA
    BMC Microbiol 9:152. 2009
    ..The objective of this study was to determine stability of transformed Salmonella Typhimurium (S. typh-lux) using three different plasmids and characterize their respective photonic properties...
  36. ncbi Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain
    T Reid
    Department of Pharmacology, Kyoto University Faculty of Medicine, Yoshida, Sakyo ku, Kyoto 606, Japan
    J Biol Chem 271:13556-60. 1996
    ..region shares approximately 30% identity with the Rho-binding domains of rhophilin and a serine/threonine kinase, PKN, two other Rho target proteins that we recently identified (Watanabe, G., Saito, Y., Madaule, P., Ishizaki, T...
  37. ncbi NF-kappaB- and C/EBPbeta-driven interleukin-1beta gene expression and PAK1-mediated caspase-1 activation play essential roles in interleukin-1beta release from Helicobacter pylori lipopolysaccharide-stimulated macrophages
    Chaitali Basak
    Department of Chemistry, Bose Institute, 93 1 Acharya Prafulla Chandra Road, Kolkata 700009, India
    J Biol Chem 280:4279-88. 2005
    ..Most important, we observed a role of Rac1/PAK1 signaling in activation of caspase-1, which is necessary for maturation of pro-IL-1beta. H...
  38. ncbi Integrin engagement differentially modulates epithelial cell motility by RhoA/ROCK and PAK1
    Hua Zhou
    Department of Cell and Tissue Biology, University of California, San Francisco, California 94143, USA
    J Biol Chem 280:10624-35. 2005
    ..Cdc42 and Rac and their downstream target PAK1 were activated following adhesion to laminin-5...
  39. pmc Validation of the p21-activated kinases as targets for inhibition in neurofibromatosis type 2
    Chunling Yi
    Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 68:7932-7. 2008
    ..schwannoma samples derived from NF2 patients showed that in a significant fraction of the tumors, the activity of Pak1 was highly elevated...
  40. pmc Inhibition of contraction and myosin light chain phosphorylation in guinea-pig smooth muscle by p21-activated kinase 1
    A Wirth
    Department of Vegetative Physiology, University of Cologne, Robert Koch Strasse 39, D 50931 Cologne, Germany
    J Physiol 549:489-500. 2003
    ..Therefore, we investigated the effect of the predominant isoform in vascular smooth muscle cells, PAK1, on contraction and phosphorylation of the regulatory light chains of myosin (r-MLC) in Triton-skinned guinea-pig ..
  41. ncbi alpha6beta4 integrin activates Rac-dependent p21-activated kinase 1 to drive NF-kappaB-dependent resistance to apoptosis in 3D mammary acini
    Julie C Friedland
    Department of Pharmacology, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Sci 120:3700-12. 2007
    ..We report that elevated (alpha6)beta4 integrin-dependent Rac-Pak1 signaling supports resistance to apoptosis in mammary acini by permitting stress-dependent activation of the p65 ..
  42. ncbi [Gustometry usefulness for the evaluation of taste sense efficiency. Part I. The range of taste substances concentrations and the result of gustometry examination]
    Ewa Klimacka-Nawrot
    Z Katedry i Zakładu Podstawowych Nauk Biomedycznych w Sosnowcu Slaskiego Uniwersytetu Medycznego w Katowicach
    Wiad Lek 60:409-14. 2007
    ..The Polish Committee of Standards (Polski Komitet Normalizacyjny--PKN) recommends the performance of sensitivity taste examinations with the use of method based on rinsing out the mouth ..
  43. pmc Silencing of p21-activated kinase attenuates vimentin phosphorylation on Ser-56 and reorientation of the vimentin network during stimulation of smooth muscle cells by 5-hydroxytryptamine
    Dale D Tang
    Center for Cardiovascular Sciences, Albany Medical College, 47 New Scotland Avenue, MC 8, ME 424, Albany, NY 12208, USA
    Biochem J 388:773-83. 2005
    ..Treatment of cells with small interfering RNA selectively down-regulated the expression of PAK1 (p21-activated kinase 1) without affecting the content of smooth muscle alpha-actin...
  44. ncbi Activation of the small GTPase Rac1 by cGMP-dependent protein kinase
    Yali Hou
    Department of Biochemistry and Molecular Biology, Medical College of Georgia, 1120 15th Street, CB2510, Augusta, GA 30912 2100, USA
    Cell Signal 16:1061-9. 2004
    ..Moreover, using in vitro kinase assays it was found that cGMP also stimulated the activity of the Rac1 effector Pak1. The activation of both Rac1 and Pak1 by 8-Br-cGMP was completely abolished by transfection of the cells with ..
  45. ncbi PAK1 primes MEK1 for phosphorylation by Raf-1 kinase during cross-cascade activation of the ERK pathway
    Lucy C Coles
    School of Biomedical Sciences, University of Nottingham Medical School, Queen s Medical Centre, Nottingham NG7 2UH, UK
    Oncogene 21:2236-44. 2002
    ..Here we show that both Raf-1 and MEK1 are phosphorylated by PAK1 and that mutations at PAK1 phosphorylation sites in either protein prevent cross-cascade activation...
  46. ncbi PKN activation via transforming growth factor-beta 1 (TGF-beta 1) receptor signaling delays G2/M phase transition in vascular smooth muscle cells
    Chang Su
    Department of Integrative Physiology, Cardiovascular Research Institute, University of North Texas Health Science Center, Fort Worth, Texas 76107, USA
    Cell Cycle 6:739-49. 2007
    ..have demonstrated that exposure of the VSMC line, PAC-1, to Transforming growth factor-beta1 (TGF-beta1), activated PKN (a RhoA-dependent kinase)...
  47. doi The rho guanosine 5'-triphosphatase, cell division cycle 42, is required for insulin-induced actin remodeling and glucagon-like peptide-1 secretion in the intestinal endocrine L cell
    Gareth E Lim
    Department of Physiology, University of Toronto, Toronto, Ontario, Canada
    Endocrinology 150:5249-61. 2009
    ..After insulin treatment, Cdc42-dependent p21-activated kinase-1 (PAK1) activation was also detected, and transfection of small-interfering RNA against Cdc42 or of dominant-negative ..
  48. ncbi p21-activated kinase (PAK1) is phosphorylated and activated by 3-phosphoinositide-dependent kinase-1 (PDK1)
    C C King
    Departments of Immunology and Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 275:41201-9. 2000
    ..we show that phosphorylated 3-phosphoinositide-dependent kinase 1 (PDK1) phosphorylates p21-activated kinase 1 (PAK1) in the presence of sphingosine...
  49. ncbi Deleted in colorectal cancer binding netrin-1 mediates cell substrate adhesion and recruits Cdc42, Rac1, Pak1, and N-WASP into an intracellular signaling complex that promotes growth cone expansion
    Masoud Shekarabi
    Center for Neuronal Survival, Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada H3A 2B4
    J Neurosci 25:3132-41. 2005
    ..growth cone expansion requires Cdc42 (cell division cycle 42), Rac1 (Ras-related C3 botulinum toxin substrate 1), Pak1 (p21-activated kinase), and N-WASP (neuronal Wiskott-Aldrich syndrome protein) and that the application of netrin-1 ..
  50. pmc Kinase-deficient Pak1 mutants inhibit Ras transformation of Rat-1 fibroblasts
    Y Tang
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    Mol Cell Biol 17:4454-64. 1997
    ..We show here that expression of a catalytically inactive mutant Pak, Pak1(R299), inhibits Ras transformation of Rat-1 fibroblasts but not of NIH 3T3 cells...
  51. ncbi Identification and characterization of PKNbeta, a novel isoform of protein kinase PKN: expression and arachidonic acid dependency are different from those of PKNalpha
    K Oishi
    Graduate School of Science and Technology, Faculty of Science, Kobe, 657 8501, Japan
    Biochem Biophys Res Commun 261:808-14. 1999
    The cDNA clone encoding a novel isoform of protein kinase PKN, termed PKNbeta, was isolated from a HeLa cDNA library...
  52. doi Kinin B1 receptor stimulation modulates leptin homeostasis. Evidence for an insulin-dependent mechanism
    Marcelo A Mori
    Department of Biophysics, Universidade Federal de Sao Paulo, Sao Paulo, Brazil
    Int Immunopharmacol 8:242-6. 2008
    ..Here we demonstrate that the intravenous administration of the kinin B1 receptor agonist des-Arg9-bradykinin (DBK) in mice leads to significant increase in serum leptin levels...
  53. ncbi Characterization of Pak2p, a pleckstrin homology domain-containing, p21-activated protein kinase from fission yeast
    M A Sells
    Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Biol Chem 273:18490-8. 1998
    ..We previously showed that fission yeast pak1+ encodes an essential protein that affects mating and cell polarity...
  54. ncbi Genetic deletion of Rac1 GTPase reveals its critical role in actin stress fiber formation and focal adhesion complex assembly
    Fukun Guo
    Division of Experimental Hematology, Children s Hospital Research Foundation, University of Cincinnati, Cincinnati, Ohio 45229, USA
    J Biol Chem 281:18652-9. 2006
    ..Although Rac1 deletion caused a significant reduction in phospho-PAK1, -AKT, and -ERK under serum stimulation, reconstitution of active PAK1, but not AKT or MEK1, was able to rescue the ..
  55. doi p21-activated kinases regulate actin remodeling in glomerular podocytes
    Jianxin Zhu
    Department of Medicine, McGill University Health Centre, 3775 University Street, Montreal, Quebec, Canada
    Am J Physiol Renal Physiol 298:F951-61. 2010
    ..Here, we investigated the role of Pak in podocyte morphology. Pak1/2 were expressed in cultured podocytes...
  56. ncbi Phosphorylated Pak1 level in the cytoplasm correlates with shorter survival time in patients with glioblastoma
    Hiroshi Aoki
    Department of Neurosurgery, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Clin Cancer Res 13:6603-9. 2007
    ..Accumulating evidence has shown that phosphorylated p21-activated kinase 1 (Pak1), a mediator of small guanosine triphosphatases, plays a role in the proliferation, survival, and invasiveness of ..
  57. ncbi p21-activated kinase-1 signaling mediates cyclin D1 expression in mammary epithelial and cancer cells
    Seetharaman Balasenthil
    Department of Molecular and Cellular Oncology, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 279:1422-8. 2004
    p21-activated kinase 1 (Pak1) has been shown recently to induce hyperplasia in the mammary epithelium, a phenotype also manifested by overexpression of cyclin D1, a known indicator of the proliferative stage...
  58. ncbi p21-activated kinase-1 (PAK1) inhibition of the human scavenger receptor class B, type I promoter in macrophages is independent of PAK1 kinase activity, but requires the GTPase-binding domain
    T G Hullinger
    Department of Cardiovascular Pharmacology, Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, MI 48105, USA
    J Biol Chem 276:46807-14. 2001
    ..7. Cotransfection with either a constitutively active p21-activated protein kinase-1 (PAK1) construct (T423E) or a kinase-deficient PAK1 construct (K299R) resulted in repression of the SR-BI promoter, ..
  59. doi Estrogen receptor-alpha phosphorylation at serine 305, nuclear p21-activated kinase 1 expression, and response to tamoxifen in postmenopausal breast cancer
    Josefine Bostner
    Division of Surgery and Clinical Oncology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linkoping University, Linkoping, Sweden
    Clin Cancer Res 16:1624-33. 2010
    In vitro, p21-activated kinase 1 (Pak1) phosphorylates the serine 305 residue of the estrogen receptor alpha (ERalpha) and influences the response of breast cancer cells to tamoxifen...
  60. ncbi p21-Activated kinase-1 and its role in integrated regulation of cardiac contractility
    Katherine A Sheehan
    Department of Physiology and Biophysics, College of Medicine, University of Illinois at Chicago, 835 S Wolcott Ave, Chicago, IL 60612 7342, USA
    Am J Physiol Regul Integr Comp Physiol 293:R963-73. 2007
    ..cardiac contractility involving variations in the activity of the multifunctional enzyme, p21-activated kinase 1 (Pak1), a member of a family of proteins in the small G protein-signaling pathway that is activated by Cdc42 and Rac1...
  61. pmc Combined array comparative genomic hybridization and tissue microarray analysis suggest PAK1 at 11q13.5-q14 as a critical oncogene target in ovarian carcinoma
    Peter Schraml
    Institute of Pathology, University Hospital, University of Basel, Schonbeinstrasse 40, 4031 Basel, Switzerland
    Am J Pathol 163:985-92. 2003
    ..DNA copy number gains were most frequently observed for PIK3CA on 3q (66%), PAK1 on 11q (59%), KRAS2 on 12p (55%), and STK15 on 20q (55%)...
  62. pmc Serine 28 phosphorylation of NRIF3 confers its co-activator function for estrogen receptor-alpha transactivation
    A H Talukder
    Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Oncogene 27:5233-42. 2008
    ..p21-activated protein kinase 1 (Pak1) phosphorylates ERalpha at Ser305 and this modification is important in ERalpha transactivation function...
  63. pmc p21-Activated kinase mediates rapid estradiol-negative feedback actions in the reproductive axis
    Zhen Zhao
    Department of Neurobiology and Physiology, Northwestern University, Evanston, Il 60208 3113, USA
    Proc Natl Acad Sci U S A 106:7221-6. 2009
    ..These studies tested the hypothesis that p21-activated kinase 1 (PAK1), a serine/threonine kinase rapidly activated by E(2) in nonneural cells, functions as a downstream node for E(2) ..
  64. pmc Identification of two eukaryote-like serine/threonine kinases encoded by Chlamydia trachomatis serovar L2 and characterization of interacting partners of Pkn1
    Anita Verma
    Department of Microbiology and Immunology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Infect Immun 71:5772-84. 2003
    ..trachomatis serovar D revealed the presence of three putative open reading frames (ORFs), CT145 (Pkn1), CT673 (Pkn5), and CT301 (PknD), encoding eukaryote-like serine/threonine kinases (Ser/Thr kinases)...
  65. pmc A member of the Ste20/PAK family of protein kinases is involved in both arrest of Xenopus oocytes at G2/prophase of the first meiotic cell cycle and in prevention of apoptosis
    S Faure
    Centre de Recherches de Biochimie Macromoleculaire, CNRS ERS 155, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    EMBO J 16:5550-61. 1997
    ..Microinjection of a catalytically inactive mutant of X-PAK1 with a K/R substitution in the ATP binding site, also deleted of its Nter-half that contains the conserved domains ..
  66. ncbi The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis
    She Chen
    Department of Biochemistry, Shanghai Medical Center, Fudan University formerly the Shanghai Medical University, People s Republic of China
    J Biol Chem 278:20029-36. 2003
    ..used the yeast two-hybrid system to screen a human fetal liver cDNA library and identified p21-activated kinase 1 (PAK1) as an interacting partner of p110C...
  67. ncbi Adhesion stimulates direct PAK1/ERK2 association and leads to ERK-dependent PAK1 Thr212 phosphorylation
    Liisa J Sundberg-Smith
    Department of Pathology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 280:2055-64. 2005
    ..Herein, we found that adhesion signaling also stimulates an association between PAK1 and ERK1/2...
  68. pmc Active p21-activated kinase 1 rescues MCF10A breast epithelial cells from undergoing anoikis
    Raymond E Menard
    Department of Pharmacology, Wayne State University, 540 East Canfield Avenue, Detroit, MI 48201, USA
    Neoplasia 7:638-45. 2005
    The protein kinase, PAK1, is overexpressed in human breast cancer and may contribute to malignancy through induction of proliferation and invasiveness...
  69. ncbi Regulation of phosphoglucomutase 1 phosphorylation and activity by a signaling kinase
    Anupama Gururaj
    Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Oncogene 23:8118-27. 2004
    ..of cross-talk between cell signaling and metabolic pathways, whereby the signaling kinase p21-activated kinase 1 (Pak1) binds to, phosphorylates and enhances the enzymatic activity of phosphoglucomutase 1 (PGM), an important ..
  70. doi PAK1 interacts with beta-catenin and is required for the regulation of the beta-catenin signalling pathway by gastrins
    Hong He
    Department of Surgery, University of Melbourne, Austin Health, Studley Road, Heidelberg, Melbourne, Victoria 3084, Australia
    Biochim Biophys Acta 1783:1943-54. 2008
    ..also regulate beta-catenin signalling through multiple pathways which seem to converge on p21-activated kinase 1 (PAK1). In this study, we have investigated the role of PAK1 in the regulation of beta-catenin signalling by gastrins...
  71. ncbi Merlin, the product of the Nf2 tumor suppressor gene, is an inhibitor of the p21-activated kinase, Pak1
    Joseph L Kissil
    Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 12:841-9. 2003
    ..We describe a novel interaction between merlin and p21-activated kinase 1 (Pak1), which is dynamic and facilitated upon increased cellular confluence...
  72. pmc p21-activated kinase 1 (Pak1) regulates cell motility in mammalian fibroblasts
    M A Sells
    Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Cell Biol 145:837-49. 1999
    The p21 (Cdc42/Rac) activated kinase Pak1 regulates cell morphology and polarity in most, if not all, eukaryotic cells...
  73. doi Membrane transport of WAVE2 and lamellipodia formation require Pak1 that mediates phosphorylation and recruitment of stathmin/Op18 to Pak1-WAVE2-kinesin complex
    Kazuhide Takahashi
    Molecular Cell Biology Division, Kanagawa Cancer Center Research Institute, Yokohama 241 0815, Japan
    Cell Signal 21:695-703. 2009
    ..We find that Pak1 constitutively binds to WAVE2 and is transported with WAVE2 to the leading edge by stimulation with hepatocyte ..
  74. ncbi The Rac1 polybasic region is required for interaction with its effector PRK1
    Rakhee Modha
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, United Kingdom
    J Biol Chem 283:1492-500. 2008
    Protein kinase C-related kinase 1 (PRK1 or PKN) is involved in regulation of the intermediate filaments of the actin cytoskeleton, as well as having effects on processes as diverse as mitotic timing and apoptosis...
  75. pmc Wnt-5a/Ca2+-induced NFAT activity is counteracted by Wnt-5a/Yes-Cdc42-casein kinase 1alpha signaling in human mammary epithelial cells
    Janna Dejmek
    Experimental Pathology, Department of Laboratory Medicine, Lund University, U MAS, Entrance 78, SE 205 02 Malmo, Sweden
    Mol Cell Biol 26:6024-36. 2006
    ..The Wnt-5a-induced inhibition of NFAT1 did not require glycogen synthase kinase 3beta, JNK, or Pak1 activity or modulation of the cytoskeleton...
  76. ncbi Insulin-like growth factor-1 regulates endogenous RUNX2 activity in endothelial cells through a phosphatidylinositol 3-kinase/ERK-dependent and Akt-independent signaling pathway
    Meng Qiao
    Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, Maryland 21201, USA
    J Biol Chem 279:42709-18. 2004
    ..However, an inhibitor of the p21-activated protein kinase-1, glutathione S-transferase-Pak1-(83-149), inhibited both basal and IGF-1-stimulated RUNX2 DNA binding, suggesting that Pak1 mediates IGF-1 ..
  77. doi P21-activated kinase 4--not just one of the PAK
    Anna E Dart
    Division of Cancer Studies, New Hunts House, Guy s Campus, King s College London, London SE1 1UL, UK
    Eur J Cell Biol 92:129-38. 2013
    ..Historically much of the attention has been directed towards founding family member PAK1 but the focus is now shifting towards PAK4...
  78. doi Differential binding of RhoA, RhoB, and RhoC to protein kinase C-related kinase (PRK) isoforms PRK1, PRK2, and PRK3: PRKs have the highest affinity for RhoB
    Catherine L Hutchinson
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, U K
    Biochemistry 52:7999-8011. 2013
    ..We have assessed the ability of the HR1a and HR1b domains from the three PRK isoforms (PRK1, PRK2, and PRK3) to interact with the three Rho isoforms (RhoA, RhoB, and RhoC)...
  79. ncbi The pathogen Pseudomonas aeruginosa negatively affects the attraction response of the nematode Caenorhabditis elegans to bacteria
    Thomas R Laws
    Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 OJQ, UK
    Microb Pathog 40:293-7. 2006
    ..aeruginosa strains PAK1 or PA01. P...
  80. doi Physiological roles of Rho and Rho effectors in mammals
    Dean Thumkeo
    Department of Pharmacology, Kyoto University Faculty of Medicine, Sakyo ku, Kyoto 606 8501, Japan Innovation Center for Immunoregulation, Technologies and Drugs AK Project, Kyoto University Graduate School of Medicine, Sakyo ku, Kyoto 606 8501, Japan Electronic address
    Eur J Cell Biol 92:303-15. 2013
    ..Upon binding GTP, Rho exerts its functions through downstream Rho effectors, such as ROCK, mDia, Citron, PKN, Rhophilin and Rhotekin...
  81. doi Axl gene knockdown inhibits the metastasis properties of hepatocellular carcinoma via PI3K/Akt-PAK1 signal pathway
    Jingchao Xu
    Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116027, Liaoning Province, China
    Tumour Biol 35:3809-17. 2014
    ..The key factor of phosphatidylinositol-3-kinase (PI3K)/Akt-p21-activated kinases-1 (PAK1) signaling pathway was studied after Axl expression was downregulated by shRNA...
  82. doi Curcumin inhibits lung cancer cell migration and invasion through Rac1-dependent signaling pathway
    Qing yong Chen
    Department of Respiratory Disease, the 117th Hospital of PLA, Hangzhou, Zhejiang 310013, P R China Electronic address
    J Nutr Biochem 25:177-85. 2014
    ..Meanwhile, we demonstrated that the suppression of invasiveness correlated with inhibition of Rac1/PAK1 signaling pathways and matrix metalloproteinase (MMP) 2 and 9 protein expression by combining curcumin treatment ..
  83. ncbi Identification of new kinase clusters required for neurite outgrowth and retraction by a loss-of-function RNA interference screen
    S H Y Loh
    MRC Toxicology Unit, University of Leicester, Leicester, UK
    Cell Death Differ 15:283-98. 2008
    ..Finally, we demonstrated that haploinsufficiency of two members of the AGC kinase subgroup, ROCK1 and PKN1, was able to suppress retinal degeneration in Drosophila model of class III Autosomal Dominant Retinitis Pigmentosa.
  84. ncbi An essential role of Pak1 phosphorylation of SHARP in Notch signaling
    Ratna K Vadlamudi
    Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Oncogene 24:4591-6. 2005
    ..The expression of both Notch and Pak1 has been shown to be deregulated in several human cancers...
  85. doi Amplification of 11q13 in ovarian carcinoma
    Lindsay A Brown
    Department of Pathology, Vancouver Coastal Health Research Institute, Genetic Pathology Evaluation Centre, British Columbia Cancer Agency, University of British Columbia, Vancouver, BC, Canada
    Genes Chromosomes Cancer 47:481-9. 2008
    ..this region led to the identification of multiple amplicons containing several potential oncogenes including EMSY, PAK1, RSF1, and GAB2...
  86. pmc Gene targeting of Cdc42 and Cdc42GAP affirms the critical involvement of Cdc42 in filopodia induction, directed migration, and proliferation in primary mouse embryonic fibroblasts
    Linda Yang
    Division of Experimental Hematology, Children s Hospital Medical Center, Molecular Developmental Biology Graduate Program, University of Cincinnati, Cincinnati, OH 45229, USA
    Mol Biol Cell 17:4675-85. 2006
    ..These defects were associated with deficiencies of PAK1, GSK3beta, myosin light chain, and FAK phosphorylation...
  87. ncbi Angiotensin II-induced activation of p21-activated kinase 1 requires Ca2+ and protein kinase C{delta} in vascular smooth muscle cells
    Elethia A Woolfolk
    Meharry Medical College, Department of Physiology, 1005 DB Todd Blvd, Nashville, TN 37208, USA
    Am J Physiol Cell Physiol 289:C1286-94. 2005
    ..However, the detailed signaling mechanism by which ANG II induces PAK1 activation in VSMCs remains unclear...
  88. pmc Phosphorylation of the ErbB3 binding protein Ebp1 by p21-activated kinase 1 in breast cancer cells
    D Akinmade
    Department of Pathology, University of Maryland, Baltimore, MD, USA
    Br J Cancer 98:1132-40. 2008
    ..The p21-activated serine/threonine kinase 1 (PAK1), which plays an important role in breast cancer progression and resistance to the anti-oestrogen tamoxifen, is ..
  89. doi Genome-wide analysis of chromosomal alterations in patients with esophageal squamous cell carcinoma exposed to tobacco and betel quid from high-risk area in India
    Indranil Chattopadhyay
    Institute of Pathology, New Delhi 110029, India
    Mutat Res 696:130-8. 2010
    ..2-13.3), TLR4 (9q32-q33), TNC (9q33), NTRK2 (9q22.1), CD44 (11p13), NCAM1 (11q23.1), TRIM29 (11q22-q23), PAK1 (11q13-q14) and RAB27A (15q15-q21...
  90. doi Distinct, but compensatory roles of PAK1 and PAK3 in spine morphogenesis
    Bernadett Boda
    Department of Basic Neuroscience, School of Medicine, University of Geneva, 1211 Geneva 4, Switzerland
    Hippocampus 18:857-61. 2008
    b>PAK1 and PAK3 belong to a family of protein kinases that are effectors of small Rho GTPases. In humans, mutations of PAK3 have been associated with mental retardation and result in in vitro studies in defects of spine morphogenesis...
  91. ncbi Regulation of AChR clustering by Dishevelled interacting with MuSK and PAK1
    Zhen G Luo
    Department of Neurobiology, Civitan International Research Center, University of Alabama at Birmingham, 1530 Third Avenue South, Birmingham, AL 35294, USA
    Neuron 35:489-505. 2002
    ..Moreover, Dvl1 interacts with downstream kinase PAK1. Agrin activates PAK, and this activation requires Dvl. Inhibition of PAK1 activity attenuates AChR clustering...
  92. pmc p21-activated kinase 1 interacts with and phosphorylates histone H3 in breast cancer cells
    Feng Li
    The University of Texas MD Anderson Cancer Center, Houston 77030, USA
    EMBO Rep 3:767-73. 2002
    Stimulation of p21-activated kinase-1 (Pak1) signaling promotes motility, invasiveness, anchorage-independent growth and abnormal mitotic assembly in human breast cancer cells...
  93. doi Snail transcription factors in keratinocytes: Enough to make your skin crawl
    Paul W Sou
    Dermatology, University of Sydney, Australia
    Int J Biochem Cell Biol 42:1940-4. 2010
    ..regulate Snails transcriptionally, via SMAD and MAPK pathways, and post-translationally, by modulating GSK3 and PAK1 activity, which determine Snail stability and intracellular location...
  94. doi Why an A-loop phospho-mimetic fails to activate PAK1: understanding an inaccessible kinase state by molecular dynamics simulations
    Yuen Wai Ng
    sGSK Group at IMCB, Astar Neuroscience Research Partnership, Proteos Building, 61 Biopolis Drive, Singapore 138673
    Structure 18:879-90. 2010
    Crystal structures of inactive PAK1(K299R) and the activation (A)-loop phospho-mimetic PAK1(T423E) have suggested that the kinase domain is in an active state regardless of activation loop status...
  95. ncbi Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin
    C Dan
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    J Biol Chem 276:32115-21. 2001
    ..assays revealed that both wild-type and constitutively active PAK4 phosphorylated LIMK1 even more strongly than PAK1, and activated PAK4 stimulated LIMK1's ability to phosphorylate cofilin...
  96. pmc PAK1 as a therapeutic target
    Julia V Kichina
    Roswell Park Cancer Institute, Department of Cell Stress Biology, Elm and Carlton Streets, Buffalo, NY 14263, USA
    Expert Opin Ther Targets 14:703-25. 2010
    P21-activated kinases (PAKs) are involved in multiple signal transduction pathways in mammalian cells. PAKs, and PAK1 in particular, play a role in such disorders as cancer, mental retardation and allergy...
  97. pmc AIF-1 expression regulates endothelial cell activation, signal transduction, and vasculogenesis
    Ying Tian
    Temple Univ School of Medicine, Rm 810, MRB, 3420 N Broad St, Philadelphia, PA 19140, USA
    Am J Physiol Cell Physiol 296:C256-66. 2009
    ..Activation of mitogen-activated protein kinase p44/42 and PAK1 was significantly reduced in siRNA ECs challenged with inflammatory stimuli...
  98. pmc PAK1-mediated activation of ERK1/2 regulates lamellipodial dynamics
    Stephen D Smith
    Ludwig Institute for Cancer Research, University College London, 91 Riding House Street, London W1W 7BS, UK
    J Cell Sci 121:3729-36. 2008
    b>PAK1 is a member of the p21-activated kinase (PAK) family of serine/threonine kinases that are activated by the Rho GTPases Rac and Cdc42, and are implicated in regulating morphological polarity, cell migration and adhesion...
  99. doi Phosphorylation of estrogen receptor alpha, serine residue 305 enhances activity
    Robin Tharakan
    Department of Cancer and Cell Biology, Vontz Center for Molecular Studies, University of Cincinnati College of Medicine, Cincinnati, OH 45267, United States
    Mol Cell Endocrinol 295:70-8. 2008
    ..Previous studies have shown that p21-activated kinase-1 (Pak1) and cyclic-AMP dependent protein kinase (PKA) can phosphorylate ERalpha at serine residue 305...
  100. ncbi Regulation of p70 S6 kinase by complex formation between the Rac guanine nucleotide exchange factor (Rac-GEF) Tiam1 and the scaffold spinophilin
    Rachel J Buchsbaum
    Department of Biochemistry, Tufts University School of Medicine, Boston, Massachussetts 02111, USA
    J Biol Chem 278:18833-41. 2003
    ..In contrast, spinophilin binding suppresses the ability of Tiam to activate Pak1, a different Rac effector...
  101. pmc Human immunodeficiency virus type 1 Tat regulates endothelial cell actin cytoskeletal dynamics through PAK1 activation and oxidant production
    Ru Feng Wu
    The University of Texas Southwestern Medical Center and the Dallas Veterans Administration Medical Center, Dallas, Texas 75216, USA
    J Virol 78:779-89. 2004
    ..Because active mutants of p21-activated kinase 1 (PAK1) induce identical changes in actin dynamics, we hypothesized that Tat exerts its cytoskeletal effects through PAK1...

Research Grants62

  1. Role of JAK2-PAK1 interaction in prolactin-dependent signaling
    Maria Diakonova; Fiscal Year: 2013
    ..Preliminary data demonstrate that the serine-threonine kinase PAK1 associates with and is Tyr phosphorylated by JAK2...
  2. RhoB in cancer pathogenesis and as a target in combinatorial therapy
    ROBERT SMALLRIDGE; Fiscal Year: 2013
    ..to the nucleus, colocalizing with p21 in a nuclear complex that includes protein kinase C-related kinase one (PRK1), a known RhoB interacting protein and serine/threonine kinase...
  3. A novel therapy for HER2 positive hormone refractory breast cancer
    Anne W Hamburger; Fiscal Year: 2010
    ..Further, depletion of EBP1 protein by shRNA or inactivation of EBP1 by phosphorylation by PAK1, a kinase implicated in both breast cancer progression and tamoxifen resistance, results in development of the ..
  4. NEUROFIBROMATOSIS TYPE 1 GENE REGULATES MYELOPOIESIS
    DAVID W CLAPP; Fiscal Year: 2012
    ..We have recently shown that p21 activated kinase 1 (Pak 1), a serine/threonine kinase, is hyperactivated in Nf1 mast cells2...
  5. Pathophysiology of Conduction Block in HNPP.
    Jun Li; Fiscal Year: 2013
    ..Moreover, we have shown that removal of the p21-activated kinase type-1 (pak1) gene in PMP22 deficient mice eliminates tomacula/axonal constrictions, a novel signaling mechanism...
  6. Human pathogenic bacterium induces actin phosphorylation to selectively regulate
    Hameeda Sultana; Fiscal Year: 2012
    ..I also show that A. phagocytophilum-induced actin phosphorylation is dependent on Ixodes PAK1-PI3kinase signaling. A...
  7. Post-ischemic cardiac remodeling
    MICHELLE MONASKY; Fiscal Year: 2012
    ..we have previously shown to be cardioprotective during acute ischemia/reperfusion injury is p21-activated kinase 1 (Pak1), a serine/threonine kinase...
  8. CIB1 regulation of endothelial function
    Leslie V Parise; Fiscal Year: 2013
    ..Separate studies have shown that CIB1 binds directly to, and activates, PAK1, a serine/threonine kinase known to regulate endothelial cell migration...
  9. NFATs and Vascular Injury
    Gadiparthi N Rao; Fiscal Year: 2013
    ..HASMC growth and motility require NFATc1-cyclin D1-dependent activation of protein kinase C-related kinase 1 (PRK1) and p21-activated kinase 1 (PAK1) and 3. Smooth muscle (SM)-NFATc1 mediates vascular wall remodeling...
  10. Mechanisms of structural plasticity in stress-related disorders
    Scott J Russo; Fiscal Year: 2013
    ..are unknown, intracranial injections of the HSV-IKK mutants into the NAc resulted in gross changes in Rac1-PAK1 signaling, a RhoGTPase pathway known to mediate actin cytoskeletal reorganization and the development of new spines...
  11. GPCR Signaling &Vascular Wall Remodeling
    Gadiparthi N Rao; Fiscal Year: 2013
    ..3. Gab1 targets PAK1 in mediating thrombin-induced HASMC F-actin stress fiber formation, migration, proliferation and neointima ..
  12. Cyclic AMP Mediation of Epithelial Cell Function
    JAMES RICHARD GOLDENRING; Fiscal Year: 2012
    ..AKAP350/450 splice variants potentially scaffold PKA, protein kinase C[unreadable], PKN, casein kinase 1, phosphodiesterase 4D3, protein phosphatases 1 and 2a and calmodulin as well as a number of ..
  13. Structure-directed investigations into the regulation of Ste20 kinases
    Benjamin E Turk; Fiscal Year: 2013
    ..Type II PAKs encompass the proteins PAK4, PAK5 and PAK6. Whereas the molecular basis for how type I PAKs (PAK1, PAK2 and PAK3) are regulated by inter- molecular autoinhibition is well established, the type II PAKs are not ..
  14. TROPONIN MODULATION IN HEART FAILURE
    Beata M Wolska; Fiscal Year: 2013
    ..by applicant): In experiments proposed here we test the hypothesis that signaling through p21 activated kinase (Pak1) to protein phosphatase 2A (PP2A) is a novel mechanism of control of contractility by suppression of Ca-release ..
  15. Regulation of Myosin Phosphorylation in Smooth Muscle
    Mitsuo Ikebe; Fiscal Year: 2013
    ..have been done on the regulatory role of Rho kinase in smooth muscle, nothing is known about the function of PKN, another RhoA down-stream kinase...
  16. Regulation and function of PDK1-Akt-Pyk2 axis in platelets
    Satya P Kunapuli; Fiscal Year: 2013
    ..We propose to evaluate the role of PAK in translocation of Akt to the membrane using pharmacological approaches and PAK1 and 2 knockout mice...
  17. Pak1 Signaling and Targets in Breast Cancer Progression
    Rakesh Kumar; Fiscal Year: 2013
    ..molecule known to cause progression of breast cancer cells to more invasive phenotypes is p21-activated kinase 1 (Pak1), a nodular kinase activated by a variety of cell surface or intracellular signals...
  18. Cortical Synapses and Psychosis in AD
    Robert A Sweet; Fiscal Year: 2012
    ..spines are reduced in subjects with AD+P in comparison to subjects with AD-P;3) Effectors of LTP (Kalirin7 and PAK1) are reduced, and an effector of LTD (Calcineurin) is elevated, in AD+P compared to AD-P subjects;and 4) increased ..
  19. Role of PAK1-MORC2 Pathway in Breast Cancer
    Rakesh Kumar; Fiscal Year: 2010
    ..It is our hope that the knowledge gained from this project will form the basis for new mechanistic advances in DNAdamage response in breast cancer cells. ..
  20. Role of the serine-threonine kinase PAK1 in prolactin-dependent signaling
    Maria Diakonova; Fiscal Year: 2007
    ..Preliminary data demonstrate that the serine-threonine kinase PAK1 associates with and is Tyr phosphorylated by JAK2...
  21. Receptor Trafficking in Entry of Murine Leukemia Viruses
    Robert A Davey; Fiscal Year: 2011
    ..Genes corresponding to the Rac1-PAK1-LIMK1 pathway of actin regulation were identified as key for infection by Fr-MLV but not the other viruses...
  22. Signaling by p21-activated protein kinases
    JONATHAN D CHERNOFF; Fiscal Year: 2010
    ..The use of a specific Pak inhibitor, as well as cells derived from our recently constructed Pak1 and Pak2 knock-out mice, give us unique reagents with which to accomplish these goals...
  23. Coordination of signaling during phagocytosis
    Joel A Swanson; Fiscal Year: 2010
    ..protein (YFP) chimeras of actin, PI3K, the lipid phosphatase SHIP-1, Rac1, Cdc42, the p21- binding domain of Pak1, and 3'PI-binding domains from signaling proteins...
  24. Identification of Binding Proteins of the Xenopus Morphogenetic Factor Tumorhead
    Edwin E Traverso; Fiscal Year: 2012
    ..Recent studies suggest that TH sub-cellular localization is regulated by Xenopus p21-activated kinase 1 (X-PAK1), whose overexpression is also associated with tumor growth...
  25. Role of a Novel Homeobox Transcription Factor (HLX) in Acute Myeloid Leukemia
    Ulrich G Steidl; Fiscal Year: 2013
    ..common disease alleles, including FLT3-ITD and CBFB-MYH11, and through specific HLX-dependent pathways, including PAK1 and BTG1...
  26. Survival Mechanisms of Invasive Carcinoma Cells
    Richard L Klemke; Fiscal Year: 2013
    ..amplify tyrosine kinase activity in the cell leading to activation of the canonical ErbB2/Src/p130CAS/c-CrkII/Rac/Pak1/MEK/ERK pathway, which modulates cytoskeletal and integrin functions to drive cell locomotion, invasion, and ..
  27. PAK1's role in eotaxin-mediated murine eosinophil migration
    Muithi M Mwanthi; Fiscal Year: 2012
    ..Through actin-binding effectors, PAK1 modulates the actin cytoskeleton in mast cell degranulation, and additionally we have shown that this isoform is ..
  28. Novel therapeutic targets for Leukemia in Elderly
    Holly Martin; Fiscal Year: 2013
    ..Accordingly, I hypothesized that effectors of the PI3K signaling pathway, in particular p21 activated kinase (PAK1) and its upstream effectors including guanine exchange factors (GEF) and Rho family GTPases contribute to gain-of-..
  29. p21-Activated Kinases as New Therapeutic Targets in Neurofibromatosis Type 1
    JONATHAN D CHERNOFF; Fiscal Year: 2013
    ..vitro;and 3) We will cross Krox20-cre/Nf1flox/- mice, which develop malignant peripheral nerve sheath tumors, with Pak1 knock out mice, or transplant such NF1 mice with Pak1-/- bone marrow cells, to determine if loss of Pak1, globally ..
  30. Proteolytic Pathways in Progression of Pre-Malignant Breast Disease
    Bonnie F Sloane; Fiscal Year: 2013
    ..Aberrant signal transduction, for example through p21-activated kinase 1 [PAK1], may contribute to increased pericellular proteolysis...
  31. Oncogenic MLK3-Pak1 Signaling in ER Negative Breast Cancer
    AJAY NMN RANA; Fiscal Year: 2013
    ..via MLK3, we observed a strong interaction between mammalian homolog of yeast Ste20 member, p21 activated kinase (Pak1) and MLK3...
  32. Intracellular signaling by DSCAM during retinal development
    Andrew Garrett; Fiscal Year: 2012
    ..There are a few proteins known to interact with the Dscams: Pak1 (p21-activated kinase) can be activated by Dscam, and the MAGI (membrane- associated guanylate kinase with inverted ..
  33. Regulation of epithelial contact inhibition by Rac effector proteins
    MIRJAM M ZEGERS; Fiscal Year: 2010
    ..defined here as "Pak:PIX complexes", minimally contain the Rac/cdc42 effector molecules p21-activated kinase 1 (Pak1) and the Rac/cdc42-guanine exchange factor betaPIX...
  34. The Role of the Cdc42-Pak1 Signaling Pathway in the Incretin Effect
    STEPHANIE MICHELLE YODER; Fiscal Year: 2013
    ..Toward this, the involvement of a new signaling axis involving Cdc42-Pak1 proteins in regulating insulin-induced GLP-1 secretion in vitro was recently revealed...
  35. REGULATION OF NEUTROPHIL RECEPTOR G PROTEIN INTERACTIONS
    Gary Bokoch; Fiscal Year: 2009
    ..We identified molecular targets for cytoskeletal regulation by PAK1, including GEF-H1, LIM kinase, and myosin light chain kinase...
  36. Control of lung cancer invasion and metastasis by vimentin
    Adam I Marcus; Fiscal Year: 2013
    ..we have published that STRAD1-LKB1 interact with the canonical cell polarity and motility proteins cdc42-PAK1. We build upon this data to determine whether STRAD1-LKB1 regulate vimentin through cdc42-PAK1...
  37. Real-time detection of cancer biomarkers using a self-sustained aptazyme
    Charles Olea; Fiscal Year: 2013
    ..two aptazymes specific for cancer biomarkers prostate-specific antigen (PSA) and p21-activated kinase (PAK1) will be developed...
  38. PAK Kinases Regulate Melanoma Chemoresistance and Metastasis
    Anand K Ganesan; Fiscal Year: 2013
    ..These studies determined that RhoJ and its downstream kinase PAK1 suppress pathways in melanoma cells that sense DNA damage...
  39. The role of Pak1 in regulating plexiform neurofibroma formation
    Andrew McDaniel; Fiscal Year: 2009
    The objective of this application is to identify the role of p21-Activated Kinase 1 (Pak1) in molecular pathways responsible for specific cellular and biochemical phenotypes associated with plexiform neurofibroma formation resulting from ..
  40. MOLECULAR GENETIC ALTERATIONS IN MALIGNANT MESOTHELIOMA
    JOSEPH TESTA; Fiscal Year: 2006
    ..with NF2 inactivation, determine how merlin function is modulated by phosphorylation as an effector of Rac/Cdc42/Pak1 signaling and whether merlin expression/phosphorylation alters downstream signaling involving JNK and AP-1...
  41. DETERMINANTS OF TISSUE ESTRADIOL SENSITIVITY
    Richard Santen; Fiscal Year: 2009
    ..We will test the hypothesis that non-genomic effects of estradiol act through the Rac/PAK1 pathways to enhance the formation of membrane changes to increase motility and invasiveness of breast cancer cells...
  42. REGULATION OF A C JUN KINASE BY A NOVEL TUMOR PROMOTER
    ELIZABETH WATTENBERG; Fiscal Year: 2001
    ..of all or part of a protein kinase cascade that has recently been shown to regulate JNK activation (the Rac, Cdc42/Pak1/MEKK1/SEK1/JNK pathway). Specific Aim 3. Determine if palytoxin stimulates c-Jun transcriptional activity via JNK...
  43. FUNCTION OF THE VAV PROTOONCOGENE
    Arthur Weiss; Fiscal Year: 2004
    ..might regulate TCR signaling function, we focused on one of their downstream effectors, the serine/threonine kinase Pak1. We found that Pak1 is activated by TCR stimulation and that it contributes to TCR-induced transcriptional ..
  44. NAK, A POTENTIAL THERAPEUTIC TARGET FOR AIDS
    Arie Abo; Fiscal Year: 2000
    ..Our preliminary results suggest that NAK is co-purified with Pak1 and Nef interacts with Pak1 in-vitro and in-vivo...
  45. Protection Against Apoptosis by Scatter Factor
    Eliot Rosen; Fiscal Year: 2006
    ..period, we found that: 1) the SF protection involves signaling from c-Met -> p21Ras/PI3 kinase -> c-Akt/Pak1 -> forkhead FKHR; 2) the Grb2-associated binder Gab1, an adapter that transduces epithelial morphogenesis, ..
  46. Role of Racl in Regulated Exocytosis
    EDWARD STUENKEL; Fiscal Year: 2004
    ..the role of p35/cyclin dependent kinase 5, an interacting protein kinase that regulates PAK activation, on Rac1 and PAK1 regulation of secretory responsiveness, and 4) To determine the role of Rac1 on phosphatidylinositol 4-phosphate 5- ..
  47. Cytoskeletal Regulation of Outer Hair Cell Motility
    Federico Kalinec; Fiscal Year: 2007
    ..low and auditory frequencies, by addressing the following Specific Aims: 1 - Determine the role of the Rac1-Cdc42/PAK1/LIMK1/cofilin signaling pathway in the regulation of OHC motility, and its interaction with the RhoA/ROCK/LIMK2/..
  48. RHO GTPASE SIGNALING IN EMBRYO MORPHOGENESIS
    Jeffrey Settleman; Fiscal Year: 2003
    ..In addition to DRhoGEF2, a Drosophila homolog of the major mammalian Rho effector target kinase, PKN, was also identified...
  49. PKN1 KINASE AND TSG101 TUMOR SUPPRESSOR
    Peter Burbelo; Fiscal Year: 2002
    ..mutated in sporadic breast cancer tumors, was identified in a yeast two-hybrid screen to interact with the PKN1 kinase, an effector protein for Rho...
  50. AKT1 Oncogene in Carcinogenesis
    Jin Cheng; Fiscal Year: 2005
    ..AKT1 phosphorylates APaB and PSMB4 in vitro and in vivo. Moreover, APaB binds to PAK 1 kinase and enhances AKT1-induced cell survival and cell growth...
  51. REGULATION OF P21-ACTIVATED KINASES
    Jonathan Chernoff; Fiscal Year: 2004
    ..We have created two unique reagents: Pak1 mutants that selectively couple to a single GTPase, and phosphorylation-state specific antibodies that recognize ..
  52. Mechanism of HIV 1 Nef Action
    BORIS PETERLIN; Fiscal Year: 2006
    ..3 kinase, the guanine nucleotide exchange factor Vav, small GTPases Cdc42 and Racl, and p21 activated kinases, PAK1 and 2...
  53. MECHANISMS AND ROLES OF PDK1 IN INSULIN SIGNALING
    Feng Liu; Fiscal Year: 2003
    ..The investigator will investigate whether PDK1 is an upstream kinase for protein kinase N (PKN), a serine/threonine protein kinase that has been implicated in insulin action...
  54. CD44/VARIANT CYTOSKELETON IN BREAST CANCER PROGRESSION
    LILLY YW BOURGUIGNON; Fiscal Year: 2010
    ....
  55. Role of JAK2-PAK1 interaction in human breast cancer
    Maria Diakonova; Fiscal Year: 2008
    ..Two proteins - JAK2 and PAK1-have been implicated in breast cancer...
  56. Pak1 Regulation of Cardiac Protein Phosphorylation
    Katherine Sheehan; Fiscal Year: 2005
    ..phosphorylation of cTnl, MyBP-C, and PLB in isolated rat cardiac ventricular myocytes following activation of the Pak1 pathway by the agonists bradykinin (BK) and lysophosphatidic acid (LPA) or by adenoviral-mediated over-expression ..
  57. CD44-P185HER2 INTERACTION IN OVARIAN CANCER PROGRESSION
    LILLY BOURGUIGNON; Fiscal Year: 2007
    ..abstract_text> ..
  58. Pak1 - PIN Pathway in Breast Cancer Progression
    Rakesh Kumar; Fiscal Year: 2008
    ..As described below, our recent work indicate that growth factor-stimulation of p21-activated kinase (Pak1), a serine/threonine kinase, plays a significant role in the cell motility, invasiveness and survival, all of which ..
  59. Role of Vav family proteins in cell signaling and cancer
    XOSE BUSTELO; Fiscal Year: 2009
    ..abstract_text> ..
  60. OVARIAN STEROID REGULATION OF SEROTONIN IN PRIMATES
    CYNTHIA LOUISE BETHEA; Fiscal Year: 2010
    ..Altogether, we will show that ovarian steroids support serotonin neuron survival and we will provide pivotal underlying genomic mechanisms. ..
  61. Reactive Oxygen Species in Anti-Viral Airway Host Defense
    Jeffrey Friedman; Fiscal Year: 2008
    ..Our studies will show if oxidases are a promising target for therapeutic drug development. [unreadable] [unreadable] [unreadable] [unreadable]..
  62. B-Synuclein as a Treatment for Lewy Body Disease
    Eliezer Masliah; Fiscal Year: 2005
    ..abstract_text> ..