PIK3R4

Summary

Gene Symbol: PIK3R4
Description: phosphoinositide-3-kinase regulatory subunit 4
Alias: VPS15, p150, phosphoinositide 3-kinase regulatory subunit 4, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, phosphatidylinositol 3-kinase-associated p150, phosphoinositide 3-kinase adaptor protein, phosphoinositide-3-kinase, regulatory subunit 4, p150
Species: human
Products:     PIK3R4

Top Publications

  1. Panaretou C, Domin J, Cockcroft S, Waterfield M. Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by phosphatidylinositol transfer protein to the p150.Ptdins 3-kinase complex. J Biol Chem. 1997;272:2477-85 pubmed
    ..14, 3339-3348). Here, cDNA cloning is used to show that the amino acid sequence of this protein, termed p150, is 29.6% identical and 53% similar to the yeast Vps15p protein, an established regulator of Vps34p...
  2. Vergne I, Chua J, Deretic V. Tuberculosis toxin blocking phagosome maturation inhibits a novel Ca2+/calmodulin-PI3K hVPS34 cascade. J Exp Med. 2003;198:653-9 pubmed
    ..tuberculosis residence in vacuoles sequestered away from the bactericidal and antigen-processing organelles in infected macrophages. ..
  3. Stein M, Feng Y, Cooper K, Welford A, Wandinger Ness A. Human VPS34 and p150 are Rab7 interacting partners. Traffic. 2003;4:754-71 pubmed
    ..endosomal transport and here we establish that the phosphatidylinositol 3'-kinase hVPS34 and its adaptor protein p150 are rab7 interacting partners...
  4. Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q. Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase. Proc Natl Acad Sci U S A. 2008;105:19211-6 pubmed publisher
  5. Aktas T, Avşar Ilık İ, Maticzka D, Bhardwaj V, Pessoa Rodrigues C, Mittler G, et al. DHX9 suppresses RNA processing defects originating from the Alu invasion of the human genome. Nature. 2017;544:115-119 pubmed publisher
    ..Biochemical purifications of DHX9 identify the interferon-inducible isoform of ADAR (p150), but not the constitutively expressed ADAR isoform (p110), as an RNA-independent interaction partner...
  6. Matheson T, Kaufman P. Grabbing the genome by the NADs. Chromosoma. 2016;125:361-71 pubmed publisher
    ..including the insulator protein CCCTC-binding factor (CTCF), chromatin assembly factor (CAF)-1 subunit p150, several nucleolar proteins, and two long non-coding RNAs (lncRNAs)...
  7. Itoh Y, Higuchi M, Oishi K, Kishi Y, Okazaki T, Sakai H, et al. PDK1-Akt pathway regulates radial neuronal migration and microtubules in the developing mouse neocortex. Proc Natl Acad Sci U S A. 2016;113:E2955-64 pubmed publisher
    ..to control microtubules, likely by regulating the binding of accessory proteins including the dynactin subunit p150(glued) Consistent with this notion, we found that PDK1 regulates the expression of cytoplasmic dynein intermediate ..
  8. Poleshko A, Einarson M, Shalginskikh N, Zhang R, Adams P, Skalka A, et al. Identification of a functional network of human epigenetic silencing factors. J Biol Chem. 2010;285:422-33 pubmed publisher
    ..detected for two TRIM protein family members, the cohesin component Rad21, and the histone chaperone CHAF1A (CAF-1 p150)...
  9. Toton E, Lisiak N, Sawicka P, Rybczynska M. Beclin-1 and its role as a target for anticancer therapy. J Physiol Pharmacol. 2014;65:459-67 pubmed
    ..UVRAG, Bif-1, Rubicon, Ambra1, HMGB1, IP3R, PINK and survivin) to promote the formation of the Beclin-1-Vps34-Vps15 complex which triggers the autophagy protein cascade...

More Information

Publications109 found, 100 shown here

  1. Chen C, Hehnly H, Yu Q, Farkas D, Zheng G, Redick S, et al. A unique set of centrosome proteins requires pericentrin for spindle-pole localization and spindle orientation. Curr Biol. 2014;24:2327-2334 pubmed publisher
    ..disruption had no detectable effect on localization of proteins involved in the cortical polarity pathway (NuMA, p150(glued), aPKC)...
  2. Gannon H, Zou T, Kiessling M, Gao G, Cai D, Choi P, et al. Identification of ADAR1 adenosine deaminase dependency in a subset of cancer cells. Nat Commun. 2018;9:5450 pubmed publisher
    ..through inactivation of PKR or overexpression of either a wildtype or catalytically inactive mutant version of the p150 isoform of ADAR1, partially rescues cell lethality after ADAR1 loss, suggesting that both catalytic and non-..
  3. Yang C, Sengupta S, Hegde P, Mitra J, Jiang S, Holey B, et al. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Nucleic Acids Res. 2017;45:739-748 pubmed publisher
    ..Here we show that chromatin assembly factor (CAF)-1 subunit A (CHAF1A), the p150 subunit of the histone H3/H4 chaperone, and its partner anti-silencing function protein 1A (ASF1A), which we ..
  4. Luo Y, Lee I, Jo Y, Namgoong S, Kim N. Depletion of the LINC complex disrupts cytoskeleton dynamics and meiotic resumption in mouse oocytes. Sci Rep. 2016;6:20408 pubmed publisher
    ..Immunofluorescence results also indicated the dislocation of pericentrin and P150(Glued) after SUN1 or KASH5 depletion...
  5. Tanigawa M, Maeda T. An In Vitro TORC1 Kinase Assay That Recapitulates the Gtr-Independent Glutamine-Responsive TORC1 Activation Mechanism on Yeast Vacuoles. Mol Cell Biol. 2017;37: pubmed publisher
    ..responds to l-glutamine, resides on the vacuolar membranes, and involves a previously uncharacterized Vps34-Vps15 phosphatidylinositol (PI) 3-kinase complex and the PI-3-phosphate [PI(3)P]-binding FYVE domain-containing vacuolar ..
  6. Costa V, Esposito R, Ziviello C, Sepe R, Bim L, Cacciola N, et al. New somatic mutations and WNK1-B4GALNT3 gene fusion in papillary thyroid carcinoma. Oncotarget. 2015;6:11242-51 pubmed
    ..Using this approach we identified new missense mutations in CBL, NOTCH1, PIK3R4 and SMARCA4 genes...
  7. Guo Y, Zheng Y. Lamins position the nuclear pores and centrosomes by modulating dynein. Mol Biol Cell. 2015;26:3379-89 pubmed publisher
    ..We further show that asymmetric NPC distribution upon lamin depletion disrupts the distribution of BICD2 and p150 dynactin on the nuclear envelope at prophase, which results in inefficient dynein-driven centrosome separation ..
  8. Wang B, Her G, Hu M, Chen Y, Tung Y, Wu P, et al. ErbB2 regulates autophagic flux to modulate the proteostasis of APP-CTFs in Alzheimer's disease. Proc Natl Acad Sci U S A. 2017;114:E3129-E3138 pubmed publisher
    ..We then found that ErbB2 effectively suppressed autophagic flux by physically dissociating Beclin-1 from the Vps34-Vps15 complex independent of its kinase activity...
  9. Fokin A, Klementeva T, Nadezhdina E, Burakov A. SLK/LOSK kinase regulates cell motility independently of microtubule organization and Golgi polarization. Cytoskeleton (Hoboken). 2016;73:83-92 pubmed publisher
    ..regulator of cell locomotion that primarily acts through the small GTPase RhoA and the dynactin subunit p150(Glued)...
  10. Shibahara K, Stillman B. Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Cell. 1999;96:575-85 pubmed
    ..PCNA binds directly to p150, the largest subunit of CAF-1, and the two proteins colocalize at sites of DNA replication in cells...
  11. Boutouja F, Brinkmeier R, Mastalski T, El Magraoui F, Platta H. Regulation of the Tumor-Suppressor BECLIN 1 by Distinct Ubiquitination Cascades. Int J Mol Sci. 2017;18: pubmed publisher
    ..catalytic subunit of this complex is the lipid-kinase VPS34, which associates with the membrane-targeting factor VPS15 as well as the multivalent adaptor protein BECLIN 1...
  12. Lee K, Ahn J, Kim J, Hwang C. Synthetic lethal screen of NAA20, a catalytic subunit gene of NatB N-terminal acetylase in Saccharomyces cerevisiae. J Microbiol. 2014;52:842-8 pubmed publisher
    ..The genome-wide synthetic lethal screen of NAA20 identified genes encoding for serine/threonine protein kinase Vps15, 1,3-beta-glucanosyltransferase Gas5, and a catabolic repression regulator Mig3...
  13. Kedashiro S, Pastuhov S, Nishioka T, Watanabe T, Kaibuchi K, Matsumoto K, et al. LRRK1-phosphorylated CLIP-170 regulates EGFR trafficking by recruiting p150Glued to microtubule plus ends. J Cell Sci. 2015;128:385-96 pubmed publisher
    ..We find that LRRK1-mediated phosphorylation of CLIP-170 causes the accumulation of p150(Glued) (also known as DCTN1) a subunit of dynactin, at microtubule plus ends, thereby facilitating the migration of ..
  14. Omer S, Greenberg S, Lee W. Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1. elife. 2018;7: pubmed publisher
    ..ER- and mitochondria-independent population of Num1, dynein motor activity, and CAP-Gly domain of dynactin Nip100/p150Glued subunit...
  15. Pathak A, Stanley E, Hickman F, Wallace N, Brewer B, Li D, et al. Retrograde Degenerative Signaling Mediated by the p75 Neurotrophin Receptor Requires p150Glued Deacetylation by Axonal HDAC1. Dev Cell. 2018;46:376-387.e7 pubmed publisher
    ..HDAC1 deacetylates dynactin subunit p150Glued, which enhances its interaction with dynein...
  16. McKenney R, Huynh W, Vale R, Sirajuddin M. Tyrosination of α-tubulin controls the initiation of processive dynein-dynactin motility. EMBO J. 2016;35:1175-85 pubmed publisher
    ..This preference is mediated by dynactin's microtubule-binding p150 subunit rather than dynein itself...
  17. Kononenko N, Claßen G, Kuijpers M, Puchkov D, Maritzen T, Tempes A, et al. Retrograde transport of TrkB-containing autophagosomes via the adaptor AP-2 mediates neuronal complexity and prevents neurodegeneration. Nat Commun. 2017;8:14819 pubmed publisher
    ..b>p150Glued/dynactin-dependent transport of TrkB-containing autophagosomes requires their association with the ..
  18. Crowder M, Flynn J, McNally K, Cortes D, Price K, Kuehnert P, et al. Dynactin-dependent cortical dynein and spherical spindle shape correlate temporally with meiotic spindle rotation in Caenorhabditis elegans. Mol Biol Cell. 2015;26:3030-46 pubmed publisher
    ..We also present evidence that activation of spindle rotation is promoted by dephosphorylation of the basic domain of p150 dynactin.
  19. Goldspink D, Rookyard C, Tyrrell B, Gadsby J, Perkins J, Lund E, et al. Ninein is essential for apico-basal microtubule formation and CLIP-170 facilitates its redeployment to non-centrosomal microtubule organizing centres. Open Biol. 2017;7: pubmed publisher
    ..requirement of CLIP-170 for ninein recruitment to n-MTOCs, with possible compensation by other anchoring factors such as p150Glued and CAMSAP2 ensuring apico-basal microtubule formation despite loss of ninein at n-MTOCs.
  20. Melani M, Valko A, Romero N, Aguilera M, Acevedo J, Bhujabal Z, et al. Zonda is a novel early component of the autophagy pathway in Drosophila. Mol Biol Cell. 2017;28:3070-3081 pubmed publisher
    ..Zonda nucleation depends on Atg1, Atg13, and Atg17 but does not require Vps34, Vps15, Atg6, or Atg14. Zonda interacts physically with Atg1 through its kinase domain, as well as with Atg6 and Vps34...
  21. Montes de Oca R, Gurard Levin Z, Berger F, Rehman H, Martel E, Corpet A, et al. The histone chaperone HJURP is a new independent prognostic marker for luminal A breast carcinoma. Mol Oncol. 2015;9:657-74 pubmed publisher
    ..Several chromatin regulators, including the histone chaperones CAF-1 (subunits p150 and p60), ASF1b, and HJURP, and the centromeric histone variant CENP-A, associated with local and metastatic ..
  22. Shull A, Latham Schwark A, Ramasamy P, Leskoske K, Oroian D, Birtwistle M, et al. Novel somatic mutations to PI3K pathway genes in metastatic melanoma. PLoS ONE. 2012;7:e43369 pubmed publisher
    ..Interestingly, several novel PI3K pathway mutations were discovered in MTOR, IRS4, PIK3R1, PIK3R4, PIK3R5, and NFKB1...
  23. Alberobello A, Wang Y, Beerkens F, Conforti F, McCutcheon J, Rao G, et al. PI3K as a Potential Therapeutic Target in Thymic Epithelial Tumors. J Thorac Oncol. 2016;11:1345-1356 pubmed publisher
    ..thymic carcinoma samples, including two catalytic subunits (PIK3CA and PIK3CG) and another regulatory subunit (PIK3R4)...
  24. Adams A, Weinman S, Wozniak A. Caspase-1 regulates cellular trafficking via cleavage of the Rab7 adaptor protein RILP. Biochem Biophys Res Commun. 2018;503:2619-2624 pubmed publisher
    ..The combination of cleavage and phosphorylation were both needed for release from the dynein component p150Glued and redistribution of CD63+ve intracellular vesicles.
  25. Fransen J, Pangeni G, Pyle I, McCall M. Functional changes in Tg P23H-1 rat retinal responses: differences between ON and OFF pathway transmission to the superior colliculus. J Neurophysiol. 2015;114:2368-75 pubmed publisher
    ..At P150, metabotropic glutamate receptor 6 (mGluR6) expression is lower than in WT, consistent with a decrease in ON RGC ..
  26. Wang Z, Zhang H, Yang H, Wang S, Rong E, Pei W, et al. Genome-wide association study for wool production traits in a Chinese Merino sheep population. PLoS ONE. 2014;9:e107101 pubmed publisher
    ..43% of the significant SNP markers were located within known or predicted genes, including YWHAZ, KRTCAP3, TSPEAR, PIK3R4, KIF16B, PTPN3, GPRC5A, DDX47, TCF9, TPTE2, EPHA5 and NBEA genes...
  27. Otte R, Donkers F, Braeken M, Van den Bergh B. Multimodal processing of emotional information in 9-month-old infants II: prenatal exposure to maternal anxiety. Brain Cogn. 2015;95:107-17 pubmed publisher
    ..A trend for a positive association between P150 amplitudes and maternal anxiety scores during pregnancy may suggest these infants are more easily aroused by and ..
  28. Zhu Q, Wani G, Arab H, El Mahdy M, Ray A, Wani A. Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites. DNA Repair (Amst). 2009;8:262-73 pubmed publisher
    ..Knockdown of CAF-1 p60 abolished CAF-1 as well as uH2A foci formation. CAF-1 p150 was found to associate with NER factors TFIIH, RPA p70 and PCNA in chromatin...
  29. Estrem C, Fees C, Moore J. Dynein is regulated by the stability of its microtubule track. J Cell Biol. 2017;216:2047-2058 pubmed publisher
    ..a mechanism involving the shoulder subcomplex and the cytoskeletal-associated protein glycine-rich domain of Nip100/p150glued Our results support a model in which dynein destabilizes its microtubule substrate by using its ..
  30. Qiu R, Zhang J, Xiang X. p25 of the dynactin complex plays a dual role in cargo binding and dynactin regulation. J Biol Chem. 2018;293:15606-15619 pubmed publisher
    ..cargo adapters are implicated in dynein activation via binding to dynactin's pointed end to switch the conformation of p150, a major dynactin component, our results suggest p25 as a critical pointed-end protein involved in this process.
  31. Vodicska B, Cerikan B, Schiebel E, Hoffmann I. MISP regulates the IQGAP1/Cdc42 complex to collectively orchestrate spindle orientation and mitotic progression. Sci Rep. 2018;8:6330 pubmed publisher
    ..acts downsteam of MISP in regulating astral microtubule dynamics and the localization of the dynactin subunit p150glued that is crucial for proper spindle positioning...
  32. Fogerty J, Denton K, Perkins B. Mutations in the Dynein1 Complex are Permissible for Basal Body Migration in Photoreceptors but Alter Rab6 Localization. Adv Exp Med Biol. 2016;854:209-15 pubmed publisher
    ..and mike oko mutants, which carry mutations in the heavy chain subunit of cytoplasmic dynein 1 (dync1h1) and the p150(Glued) subunit of Dynactin (dctn1a)...
  33. Heep M, Mach P, Reautschnig P, Wettengel J, Stafforst T. Applying Human ADAR1p110 and ADAR1p150 for Site-Directed RNA Editing-G/C Substitution Stabilizes GuideRNAs against Editing. Genes (Basel). 2017;8: pubmed publisher
    ..the current guideRNA design is already able to recruit another human deaminase, ADAR1, in both isoforms, p110 and p150. However, further optimization seems necessary as the current design is less efficient for ADAR1 isoforms...
  34. Reddy B, Mattson M, Wynne C, Vadpey O, Durra A, Chapman D, et al. Load-induced enhancement of Dynein force production by LIS1-NudE in vivo and in vitro. Nat Commun. 2016;7:12259 pubmed publisher
    ..blocked by RNAi for the cytoplasmic dynein regulators LIS1 and NudE/L (Nde1/Ndel1), but not for the dynactin p150(Glued) subunit...
  35. Lee H, Komarova Y, Nadezhdina E, Anjum R, Peloquin J, Schober J, et al. Phosphorylation controls autoinhibition of cytoplasmic linker protein-170. Mol Biol Cell. 2010;21:2661-73 pubmed publisher
    ..N and C termini results in autoinhibition of CLIP-170, thus altering its binding to MTs and the dynactin subunit p150(Glued) (J. Cell Biol. 2004: 166, 1003-1014)...
  36. Villarin J, McCurdy E, Martinez J, Hengst U. Local synthesis of dynein cofactors matches retrograde transport to acutely changing demands. Nat Commun. 2016;7:13865 pubmed publisher
    ..of large vesicles requires local synthesis of Lis1, while smaller signalling endosomes require both Lis1 and p150Glued...
  37. Lee S, Ou D, Lee C, Juan L. Gene-specific transcriptional activation mediated by the p150 subunit of the chromatin assembly factor 1. J Biol Chem. 2009;284:14040-9 pubmed publisher
    Chromatin assembly factor 1 contains three subunits, p150, p60, and p48. It is essential for coupling nucleosome assembly to newly synthesized DNA...
  38. Cachat A, Alais S, Chevalier S, Journo C, Fusil F, Dutartre H, et al. ADAR1 enhances HTLV-1 and HTLV-2 replication through inhibition of PKR activity. Retrovirology. 2014;11:93 pubmed publisher
    ..ADAR1-p150 is an Interferon Stimulated Gene (ISG) induced by IFN-I that can edit viral RNAs...
  39. Pakdel A, Mounier C, Klein E, Hleibieh K, Monsion B, Mutterer J, et al. On the interaction and localization of the beet necrotic yellow vein virus replicase. Virus Res. 2015;196:94-104 pubmed publisher
    ..BNYVV RNA-1 encodes a non-structural p237 polyprotein processed in two proteins (p150 and p66) by a cis-acting protease activity...
  40. Reidick C, El Magraoui F, Meyer H, Stenmark H, Platta H. Regulation of the Tumor-Suppressor Function of the Class III Phosphatidylinositol 3-Kinase Complex by Ubiquitin and SUMO. Cancers (Basel). 2014;7:1-29 pubmed publisher
    ..subunit of the PI3K-III complex, the lipid-kinase VPS34, requires the presence of the membrane-targeting factor VPS15 as well as the adaptor protein Beclin 1...
  41. Jayappa K, Ao Z, Wang X, Mouland A, Shekhar S, Yang X, et al. Human immunodeficiency virus type 1 employs the cellular dynein light chain 1 protein for reverse transcription through interaction with its integrase protein. J Virol. 2015;89:3497-511 pubmed publisher
    ..host dynein adapter proteins such as dynein light chain 1 (DYNLL1), dynein light chain Tctex-type 1 (DYNLT1), and p150(Glued) in early steps of human immunodeficiency virus type 1 (HIV-1) replication...
  42. Chen T, Syu J, Han T, Cheng H, Lu F, Wang C. Cell Cycle-Dependent Localization of Dynactin Subunit p150 glued at Centrosome. J Cell Biochem. 2015;116:2049-60 pubmed publisher
    b>p150(glued) is the largest subunit of dynactin protein complex, through which cargo vesicles link to the microtubule minus-end directed motor protein dynein...
  43. Yu J, Lai C, Shim H, Xie C, Sun L, Long C, et al. Genetic ablation of dynactin p150Glued in postnatal neurons causes preferential degeneration of spinal motor neurons in aged mice. Mol Neurodegener. 2018;13:10 pubmed publisher
    Dynactin p150Glued, the largest subunit of the dynactin macromolecular complex, binds to both microtubules and tubulin dimers through the N-terminal cytoskeleton-associated protein and glycine-rich (CAP-Gly) and basic domains, ..
  44. Matheson T, Kaufman P. The p150N domain of chromatin assembly factor-1 regulates Ki-67 accumulation on the mitotic perichromosomal layer. Mol Biol Cell. 2017;28:21-29 pubmed publisher
    Chromatin assembly factor 1 (CAF-1) deposits histones during DNA synthesis. The p150 subunit of human CAF-1 contains an N-terminal domain (p150N) that is dispensable for histone deposition but promotes the localization of specific loci (..
  45. Park S, Kang M, Choi Y, Kim Y, Antika L, Kim D, et al. α-Asarone blocks 7β-hydroxycholesterol-exposed macrophage injury through blocking elF2α phosphorylation and prompting beclin-1-dependent autophagy. Oncotarget. 2017;8:7370-7383 pubmed publisher
    ..this compound promoted the induction of the key autophagic proteins of beclin-1, vacuolar protein sorting 34 and p150 responsible for vesicle nucleation, and prompted the conversion of microtubule-associated protein 1A/1B-light chain ..
  46. Yu X, Long Y, Shen H. Differential regulatory functions of three classes of phosphatidylinositol and phosphoinositide 3-kinases in autophagy. Autophagy. 2015;11:1711-28 pubmed publisher
    ..In contrast, the class III PtdIns3K catalytic subunit PIK3C3/Vps34 forms a protein complex with BECN1 and PIK3R4 and produces phosphatidylinositol 3-phosphate (PtdIns3P), which is required for the initiation and progression of ..
  47. Segura F, Sánchez Cano A, Jarabo S, López de la Fuente C, Cuenca N, Villegas Pérez M, et al. Assessment of Visual and Chromatic Functions in a Rodent Model of Retinal Degeneration. Invest Ophthalmol Vis Sci. 2015;56:6275-83 pubmed publisher
    ..Pigmented P23H rats were studied by optomotor testing and electroretinogram (ERG) recordings at P30, P150, and P240. Pigmented LE rats were used as a normal wild-type control. Stimuli were modified with colored filters...
  48. Pestal K, Funk C, Snyder J, Price N, Treuting P, Stetson D. Isoforms of RNA-Editing Enzyme ADAR1 Independently Control Nucleic Acid Sensor MDA5-Driven Autoimmunity and Multi-organ Development. Immunity. 2015;43:933-44 pubmed publisher
    ..We showed that the p150 isoform of ADAR1 uniquely regulated the MDA5 pathway, whereas both the p150 and p110 isoforms contributed to ..
  49. Schenk S, Minda J, Lech R, Suchan B. Out of sight, out of mind: Categorization learning and normal aging. Neuropsychologia. 2016;91:222-233 pubmed publisher
    ..The categorization of exceptions was accompanied by higher P150, P250 and P300 amplitudes...
  50. Lacruz M, Kluttig A, Tiller D, Medenwald D, Giegling I, Rujescu D, et al. Cardiovascular Risk Factors Associated With Blood Metabolite Concentrations and Their Alterations During a 4-Year Period in a Population-Based Cohort. Circ Cardiovasc Genet. 2016;9:487-494 pubmed publisher
    ..One hundred and sixty-three metabolites were measured in serum samples with the AbsoluteIDQ kit p150 (Biocrates) following a targeted metabolomics approach, in a population-based cohort of 1030 individuals, aged 45 ..
  51. Sakurai M, Shiromoto Y, Ota H, Song C, Kossenkov A, Wickramasinghe J, et al. ADAR1 controls apoptosis of stressed cells by inhibiting Staufen1-mediated mRNA decay. Nat Struct Mol Biol. 2017;24:534-543 pubmed publisher
    Both p150 and p110 isoforms of ADAR1 convert adenosine to inosine in double-stranded RNA (dsRNA). ADAR1p150 suppresses the dsRNA-sensing mechanism that activates MDA5-MAVS-IFN signaling in the cytoplasm...
  52. Gstrein T, Edwards A, Přistoupilová A, Leca I, Breuss M, Pilat Carotta S, et al. Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. Nat Neurosci. 2018;21:207-217 pubmed publisher
    ..We show that this is caused by a hypomorphic mutation in Vps15 that perturbs endosomal-lysosomal trafficking and autophagy, resulting in an upregulation of Nischarin, which ..
  53. Flores Rodriguez N, Rogers S, Kenwright D, Waigh T, Woodman P, Allan V. Roles of dynein and dynactin in early endosome dynamics revealed using automated tracking and global analysis. PLoS ONE. 2011;6:e24479 pubmed publisher
    ..For these, it requires an intact dynactin complex to allow membrane-bound p150(Glued) to activate dynein, since p50 over-expression, which disrupts the dynactin complex, inhibits inward movement ..
  54. Smith C, Matheson T, Trombly D, Sun X, Campeau E, Han X, et al. A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli. Mol Biol Cell. 2014;25:2866-81 pubmed publisher
    ..Here we present a novel role for the human p150 subunit in regulating nucleolar macromolecular interactions...
  55. George C, Ramaswami G, Li J, Samuel C. Editing of Cellular Self-RNAs by Adenosine Deaminase ADAR1 Suppresses Innate Immune Stress Responses. J Biol Chem. 2016;291:6158-68 pubmed publisher
    ..IFN treatment of Adar1(-/-) cells lacking both the p110 constitutive and p150 IFN-inducible ADAR1 proteins induced formation of stress granules, whereas neither wild-type (WT) nor Adar2(-/-) ..
  56. Ramos Goicoa M, Galdo Alvarez S, Diaz F, Zurrón M. Effect of Normal Aging and of Mild Cognitive Impairment on Event-Related Potentials to a Stroop Color-Word Task. J Alzheimers Dis. 2016;52:1487-501 pubmed publisher
    ..Aging was associated with a) slowing of the neural processing of the stimuli (P150, N2, and P3b latencies were longer), b) greater activation of the motor cortex for response preparation (LRP-R ..
  57. Gustavsson E, Trinh J, Guella I, Szu Tu C, Khinda J, Lin C, et al. DCTN1 p.K56R in progressive supranuclear palsy. Parkinsonism Relat Disord. 2016;28:56-61 pubmed publisher
    Mutations in dynactin DCTN1 (p150(glued)) have previously been linked to familial motor neuron disease or Perry syndrome (PS) consisting of depression, parkinsonism and hypoventilation...
  58. Stein K, Winters C, Chiang H. Vps15p regulates the distribution of cup-shaped organelles containing the major eisosome protein Pil1p to the extracellular fraction required for endocytosis of extracellular vesicles carrying metabolic enzymes. Biol Cell. 2017;109:190-209 pubmed publisher
    ..The PI3K regulator Vps15p is critical for the fast internalisation of extracellular vesicles. VPS15 regulates the distribution of the 100-300 nm organelles that contain the major eisosome protein Pil1p to the ..
  59. Sharadamma N, Harshavardhana Y, Ravishankar A, Anand P, Chandra N, Muniyappa K. Molecular Dissection of Mycobacterium tuberculosis Integration Host Factor Reveals Novel Insights into the Mode of DNA Binding and Nucleoid Compaction. Biochemistry. 2015;54:4142-60 pubmed publisher
    ..the presence of Arg170, Arg171, and Arg173, which might be involved in DNA binding, and a conserved proline (P150) in the tight turn...
  60. Dumas A, Lê Bury G, Marie Anaïs F, Herit F, Mazzolini J, Guilbert T, et al. The HIV-1 protein Vpr impairs phagosome maturation by controlling microtubule-dependent trafficking. J Cell Biol. 2015;211:359-72 pubmed publisher
    ..Our data reveal that Vpr interacted with EB1, p150(Glued), and dynein heavy chain and was sufficient to critically alter the microtubule plus end localization of EB1 ..
  61. Fujita N, Tobe M, Tsukamoto N, Saito S, Obata H. A randomized placebo-controlled study of preoperative pregabalin for postoperative analgesia in patients with spinal surgery. J Clin Anesth. 2016;31:149-53 pubmed publisher
    ..randomly assigned to receive diazepam 5 mg as an active placebo (D5), pregabalin 75 mg (P75), or pregabalin 150 mg (P150). The study drug was orally administered 2 hours prior to surgery and a standard anesthetic technique was used...
  62. Stoetzel C, Bär S, De Craene J, Scheidecker S, Etard C, Chicher J, et al. A mutation in VPS15 (PIK3R4) causes a ciliopathy and affects IFT20 release from the cis-Golgi. Nat Commun. 2016;7:13586 pubmed publisher
    ..Here, we identify a missense mutation in PIK3R4 (phosphoinositide 3-kinase regulatory subunit 4, named VPS15) in a family with a ciliopathy phenotype...
  63. Konno T, Ross O, Teive H, Sławek J, Dickson D, Wszolek Z. DCTN1-related neurodegeneration: Perry syndrome and beyond. Parkinsonism Relat Disord. 2017;41:14-24 pubmed publisher
    ..The mutated causative gene for PS is DCTN1, which encodes the dynactin subunit p150Glued...
  64. Mishima T, Deshimaru M, Watanabe T, Kubota K, Kinoshita Kawada M, Yuasa Kawada J, et al. Behavioral defects in a DCTN1G71A transgenic mouse model of Perry syndrome. Neurosci Lett. 2018;666:98-103 pubmed publisher
    ..These included G71A in the microtubule-binding cytoskeleton-associated protein Gly-rich domain of p150Glued...
  65. Reese B, Bachman K, Baylin S, Rountree M. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1. Mol Cell Biol. 2003;23:3226-36 pubmed
    ..In this study, we demonstrate that MBD1 partners with the p150 subunit of chromatin assembly factor 1 (CAF-1), forming a multiprotein complex that also contains HP1alpha...
  66. Zhao Y, Wang Q, Qiu G, Zhou S, Jing Z, Wang J, et al. RACK1 Promotes Autophagy by Enhancing the Atg14L-Beclin 1-Vps34-Vps15 Complex Formation upon Phosphorylation by AMPK. Cell Rep. 2015;13:1407-1417 pubmed publisher
    ..Although adaptors have been demonstrated to facilitate the assembly of the Atg14L-Beclin 1-Vps34-Vps15 complex, which functions in autophagosome formation, it remains unknown whether the autophagy machinery actively ..
  67. Pinedo P, Melendez P, Paudyal S, Krauss R, Arias F, Lopez H, et al. Association between disease occurrence and fertility of dairy cows in three geographic regions of Chile. Theriogenology. 2016;86:817-23 pubmed publisher
    ..The odds (95% confidence interval) of pregnancy at 150 DIM (P150) and the odds of survival until 150 DIM (S150) for cows that had at least one disease event within 50 DIM were 0...
  68. Sung C, Yim H. Roles of SALL2 in tumorigenesis. Arch Pharm Res. 2017;40:146-151 pubmed publisher
    The proteins p150Sal2 (product of SALL2) and p53 share growth arrest and pro-apoptotic functions by independently inducing p21Cip1/Waf1 and BAX, and both proteins are targeted by the human papilloma virus E6 protein, ..
  69. Rolef Ben Shahar T, Castillo A, Osborne M, Borden K, Kornblatt J, Verreault A. Two fundamentally distinct PCNA interaction peptides contribute to chromatin assembly factor 1 function. Mol Cell Biol. 2009;29:6353-65 pubmed publisher
    ..Here we report that the large subunit of human CAF-1 (p150) contains two distinct PCNA interaction peptides (PIPs)...
  70. Nemazanyy I, Montagnac G, Russell R, Morzyglod L, Burnol A, Guan K, et al. Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling. Nat Commun. 2015;6:8283 pubmed publisher
    ..Acute and chronic depletion of hepatic Vps15, the regulatory subunit of class III PI3K, increases insulin sensitivity and Akt signalling, an effect that ..
  71. Ohashi Y, Soler N, García Ortegón M, Zhang L, Kirsten M, Perisic O, et al. Characterization of Atg38 and NRBF2, a fifth subunit of the autophagic Vps34/PIK3C3 complex. Autophagy. 2016;12:2129-2144 pubmed
    ..its human ortholog NRBF2, accessory components of complex I consisting of Vps15-Vps34-Vps30/Atg6-Atg14 (yeast) and PIK3R4/VPS15-PIK3C3/VPS34-BECN1/Beclin 1-ATG14 (human)...
  72. Smylla T, Preiss A, Maier D. In vivo analysis of internal ribosome entry at the Hairless locus by genome engineering in Drosophila. Sci Rep. 2016;6:34881 pubmed publisher
    ..ribosome entry site in the full length H transcript resulting in two H protein isoforms (Hp120 and Hp150). Hence, H may repress Notch signalling activity in situations where cap-dependent translation is inhibited...
  73. Farshidfar F, Kopciuk K, Hilsden R, McGregor S, Mazurak V, Buie W, et al. A quantitative multimodal metabolomic assay for colorectal cancer. BMC Cancer. 2018;18:26 pubmed publisher
    ..The well-characterized Biocrates P150 kit was used to quantify 163 metabolites in patients with CRC (N = 62), adenoma (N = 31), and age- and gender-..
  74. Askham J, Vaughan K, Goodson H, Morrison E. Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome. Mol Biol Cell. 2002;13:3627-45 pubmed
    ..EB1 formed mutually exclusive complexes with APC and dynactin, and a direct interaction between EB1 and p150(Glued) was identified...
  75. Su H, Liu W. PIK3C3/VPS34 control by acetylation. Autophagy. 2017;: pubmed publisher
    ..So far, facilitating the assembly of the PIK3C3/VPS34-BECN1-PIK3R4/VPS15/p150 core complex at distinct membranes is the only known way to activate PIK3C3/VPS34 in cells...
  76. Takeshita F, Gursel I, Ishii K, Suzuki K, Gursel M, Klinman D. Signal transduction pathways mediated by the interaction of CpG DNA with Toll-like receptor 9. Semin Immunol. 2004;16:17-22 pubmed
  77. Fogel A, Dlouhy B, Wang C, Ryu S, Neutzner A, Hasson S, et al. Role of membrane association and Atg14-dependent phosphorylation in beclin-1-mediated autophagy. Mol Cell Biol. 2013;33:3675-88 pubmed publisher
    ..We map these novel phosphorylation sites to serines 90 and 93 and demonstrate that phosphorylation at these sites is necessary for maximal autophagy. These results help clarify the mechanism of beclin-1 and Atg14 during autophagy. ..
  78. Ma B, Liu B, Cao W, Gao C, Qi Z, Ning Y, et al. The Wnt Signaling Antagonist Dapper1 Accelerates Dishevelled2 Degradation via Promoting Its Ubiquitination and Aggregate-induced Autophagy. J Biol Chem. 2015;290:12346-54 pubmed publisher
    ..Our findings suggest that Dpr1 promotes the ubiquitination of Dvl2 by pVHL and mediates the protein aggregate-elicited autophagy initiation. ..
  79. Cao Y, Wang Y, Abi Saab W, Yang F, Pessin J, Backer J. NRBF2 regulates macroautophagy as a component of Vps34 Complex I. Biochem J. 2014;461:315-22 pubmed publisher
    ..The autophagic functions of Vps34 require its recruitment to a complex that includes Vps15, Beclin-1 and Atg14L (autophagy-related 14-like protein) and is known as Vps34 Complex I...
  80. Barbosa D, Duro J, Prevo B, Cheerambathur D, Carvalho A, Gassmann R. Dynactin binding to tyrosinated microtubules promotes centrosome centration in C. elegans by enhancing dynein-mediated organelle transport. PLoS Genet. 2017;13:e1006941 pubmed publisher
    ..Here, we dissect the role of dynactin's microtubule binding activity, located in the p150 CAP-Gly domain and an adjacent basic patch, in the C. elegans zygote...
  81. Matsunaga K, Saitoh T, Tabata K, Omori H, Satoh T, Kurotori N, et al. Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages. Nat Cell Biol. 2009;11:385-96 pubmed publisher
    ..These data suggest that the Beclin 1-hVps34 complex functions in two different steps of autophagy by altering the subunit composition. ..
  82. Thoresen S, Pedersen N, Liestøl K, Stenmark H. A phosphatidylinositol 3-kinase class III sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1 regulates cytokinesis and degradative endocytic traffic. Exp Cell Res. 2010;316:3368-78 pubmed publisher
    ..combined with siRNA-mediated depletion of individual subunits indicated that a specific sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1 regulates both receptor degradation and cytokinesis, whereas ATG14L, a PI3K-III ..
  83. Nascimbeni A, Fanin M, Angelini C, Sandri M. Autophagy dysregulation in Danon disease. Cell Death Dis. 2017;8:e2565 pubmed publisher
    ..like transcription factor EB (TFEB), a master player in lysosomal biogenesis, and vacuolar protein sorting 15 (VPS15), a critical factor for autophagosome and endosome biogenesis and trafficking...
  84. Rutledge R, Ghislain M, Mullins J, de Thozée C, Golin J. Pdr5-mediated multidrug resistance requires the CPY-vacuolar sorting protein Vps3: are xenobiotic compounds routed from the vacuole to plasma membrane transporters for efflux?. Mol Genet Genomics. 2008;279:573-83 pubmed publisher
    ..A similar phenotype is also observed with deletions of VPS15, VPS34, and VPS38, which encode essential members of the carboxypeptidase Y vacuolar protein-sorting pathway...
  85. Luciani A, Villella V, Esposito S, Brunetti Pierri N, Medina D, Settembre C, et al. Defective CFTR induces aggresome formation and lung inflammation in cystic fibrosis through ROS-mediated autophagy inhibition. Nat Cell Biol. 2010;12:863-75 pubmed publisher
    ..Restoring beclin 1 or knocking down p62 rescued the trafficking of CFTR(F508del) to the cell surface. These data link the CFTR defect to autophagy deficiency, leading to the accumulation of protein aggregates and to lung inflammation...
  86. Lu J, He L, Behrends C, Araki M, Araki K, Jun Wang Q, et al. NRBF2 regulates autophagy and prevents liver injury by modulating Atg14L-linked phosphatidylinositol-3 kinase III activity. Nat Commun. 2014;5:3920 pubmed publisher
    ..Our data reveal a key role for NRBF2 in the assembly of the specific Atg14L-Beclin 1-Vps34-Vps15 complex for autophagy induction...
  87. Kristensen L, Kristensen T, Abildgaard N, Thomassen M, Frederiksen M, Mourits Andersen T, et al. High expression of PI3K core complex genes is associated with poor prognosis in chronic lymphocytic leukemia. Leuk Res. 2015;39:555-60 pubmed publisher
    ..In the present study, we used real-time RT-PCR to analyze expression of the PIK3C3, PIK3R4, and BECN1 genes...
  88. Chanduri M, Rai A, Malla A, Wu M, Fiedler D, Mallik R, et al. Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport. Biochem J. 2016;473:3031-47 pubmed publisher
    ..target for pyrophosphorylation by IP7, and this modification promotes the interaction of the IC N-terminus with the p150(Glued) subunit of dynactin...
  89. Koyasu S. The role of PI3K in immune cells. Nat Immunol. 2003;4:313-9 pubmed
    ..The development of gene-targeted mice now enables us to examine the physiological functions of individual PI3K enzymes in the immune system in vivo. ..
  90. Ligon L, Shelly S, Tokito M, Holzbaur E. The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization. Mol Biol Cell. 2003;14:1405-17 pubmed
    ..Here we clarify the interaction between EB1 and dynactin and show that EB1 binds directly to the N-terminus of the p150(Glued) subunit. One function of a plus-end complex may be to regulate microtubule dynamics...
  91. Yan Y, Flinn R, Wu H, Schnur R, Backer J. hVps15, but not Ca2+/CaM, is required for the activity and regulation of hVps34 in mammalian cells. Biochem J. 2009;417:747-55 pubmed publisher
    ..In yeast, Vps34 is associated with a putative serine/threonine protein kinase, Vps15, which is required for Vps34p activity...
  92. Gammoh N, Florey O, Overholtzer M, Jiang X. Interaction between FIP200 and ATG16L1 distinguishes ULK1 complex-dependent and -independent autophagy. Nat Struct Mol Biol. 2013;20:144-9 pubmed publisher
    ..This study therefore identifies a previously uncharacterized interaction between the ULK1 and ATG5 complexes that can distinguish ULK1-dependent and -independent autophagy processes. ..