PIAS3

Summary

Gene Symbol: PIAS3
Description: protein inhibitor of activated STAT 3
Alias: ZMIZ5, E3 SUMO-protein ligase PIAS3, E3 SUMO-protein transferase PIAS3, protein inhibitor of activated STAT protein 3, zinc finger, MIZ-type containing 5
Species: human
Products:     PIAS3

Top Publications

  1. Iwasaki K, Hailemariam K, Tsuji Y. PIAS3 interacts with ATF1 and regulates the human ferritin H gene through an antioxidant-responsive element. J Biol Chem. 2007;282:22335-43 pubmed
    ..Subsequent yeast two-hybrid screening identified PIAS3 (protein inhibitor of activated STAT3) as an ATF1-binding protein...
  2. Kluge A, Dabir S, Vlassenbroeck I, Eisenberg R, Dowlati A. Protein inhibitor of activated STAT3 expression in lung cancer. Mol Oncol. 2011;5:256-64 pubmed publisher
    Protein Inhibitor of Activated Signal Transducer and Activators of Transcription 3 (PIAS3) is an endogenous inhibitor of STAT3 transcriptional activity...
  3. Nojiri S, Joh T, Miura Y, Sakata N, Nomura T, Nakao H, et al. ATBF1 enhances the suppression of STAT3 signaling by interaction with PIAS3. Biochem Biophys Res Commun. 2004;314:97-103 pubmed
    ..evidence that ATBF1 is a new regulatory factor for STAT3-mediated signal transduction through its interaction with PIAS3. PIAS3 was thus identified as an ATBF1-binding protein...
  4. Chung C, Liao J, Liu B, Rao X, Jay P, Berta P, et al. Specific inhibition of Stat3 signal transduction by PIAS3. Science. 1997;278:1803-5 pubmed
    ..A protein named PIAS3 (protein inhibitor of activated STAT) that binds to Stat3 was isolated and characterized...
  5. Kluge A, Dabir S, Kern J, Nethery D, Halmos B, Ma P, et al. Cooperative interaction between protein inhibitor of activated signal transducer and activator of transcription-3 with epidermal growth factor receptor blockade in lung cancer. Int J Cancer. 2009;125:1728-34 pubmed publisher
    ..However, the STAT3 protein has a number of endogenous negative regulators including PIAS3 (Protein Inhibitor of Activated STAT3)...
  6. Dabir S, Kluge A, McColl K, Liu Y, Lam M, Halmos B, et al. PIAS3 activates the intrinsic apoptotic pathway in non-small cell lung cancer cells independent of p53 status. Int J Cancer. 2014;134:1045-54 pubmed publisher
    Protein inhibitor of activated signal transducer and activator of transcription 3 (STAT3) (PIAS3) is an endogenous inhibitor of STAT3 that negatively regulates STAT3 transcriptional activity and cell growth and demonstrates limited ..
  7. Junicho A, Matsuda T, Yamamoto T, Kishi H, Korkmaz K, Saatcioglu F, et al. Protein inhibitor of activated STAT3 regulates androgen receptor signaling in prostate carcinoma cells. Biochem Biophys Res Commun. 2000;278:9-13 pubmed
    Protein inhibitor of activated STAT3 (PIAS3) is a specific inhibitor of signal transducer and activator of transcription 3 (STAT3). PIAS3 binds to STAT3 and inhibits its DNA-binding activity, and thereby STAT3-mediated gene activation...
  8. Matsuura T, Shimono Y, Kawai K, Murakami H, Urano T, Niwa Y, et al. PIAS proteins are involved in the SUMO-1 modification, intracellular translocation and transcriptional repressive activity of RET finger protein. Exp Cell Res. 2005;308:65-77 pubmed
    ..We show that RFP binds with PIAS (protein inhibitor of activated STAT) proteins, PIAS1, PIAS3, PIASxalpha and PIASy at their carboxyl-terminal region and is covalently modified by SUMO-1 (sumoylation)...
  9. Lyst M, Nan X, Stancheva I. Regulation of MBD1-mediated transcriptional repression by SUMO and PIAS proteins. EMBO J. 2006;25:5317-28 pubmed
    ..Here we show that MBD1 is a target for sumoylation by PIAS1 (Protein Inhibitors of Activated STAT 1) and PIAS3 E3 SUMO (small ubiquitin-like modifier)-ligases, at two conserved lysine residues within the C-terminus of MBD1...

More Information

Publications121 found, 100 shown here

  1. Wang L, Banerjee S. Differential PIAS3 expression in human malignancy. Oncol Rep. 2004;11:1319-24 pubmed
    STAT3 mediated signaling pathways can be inhibited by PIAS3 (protein inhibitor of activated STAT3), which was recently found to regulate protein stability and function by its SUMO (small-ubiquitin like modifiers) ligase activity in ..
  2. Ogata Y, Osaki T, Naka T, Iwahori K, Furukawa M, Nagatomo I, et al. Overexpression of PIAS3 suppresses cell growth and restores the drug sensitivity of human lung cancer cells in association with PI3-K/Akt inactivation. Neoplasia. 2006;8:817-25 pubmed
    ..Overexpression of PIAS3 not only significantly inhibited cell growth but also rendered cancer cells up to 12...
  3. Jang H, Yoon K, Shin Y, Kim J, Lee S. PIAS3 suppresses NF-kappaB-mediated transcription by interacting with the p65/RelA subunit. J Biol Chem. 2004;279:24873-80 pubmed
    ..using the Rel homology domain (RHD) of the p65 subunit (RelA) of NF-kappaB as bait, led to the isolation of PIAS3, previously identified as a specific inhibitor of STAT3...
  4. Dabir S, Kluge A, Aziz M, Houghton J, Dowlati A. Identification of STAT3-independent regulatory effects for protein inhibitor of activated STAT3 by binding to novel transcription factors. Cancer Biol Ther. 2011;12:139-51 pubmed
    Protein Inhibitor of Activated Signal Transducer and Activators of Transcription 3 (PIAS3) is a molecule that regulates STAT3 and has antiproliferative properties. Glioblastoma and squamous cell lung cancer lack PIAS3 expression...
  5. Schou J, Kelstrup C, Hayward D, Olsen J, Nilsson J. Comprehensive identification of SUMO2/3 targets and their dynamics during mitosis. PLoS ONE. 2014;9:e100692 pubmed publisher
    ..RhoGDIα as a major SUMO2/3 modified protein, specifically during mitosis, mediated by the SUMO ligases PIAS2 and PIAS3. Our data provide a rich resource for further exploring the role of SUMO2/3 modifications in mitosis and cell ..
  6. Kim E, Kwon K, Lee M, Park J, Ahn J. Sumoylation of a small isoform of NFATc1 is promoted by PIAS proteins and inhibits transactivation activity. Biochem Biophys Res Commun. 2019;513:172-178 pubmed publisher
    ..NFATc1/A sumoylation increased with low level expression of SUMO E3 ligases, specifically PIAS1, PIAS3, and PIASy, in co-transfected cells...
  7. Liu L, Li H, Cheng X, Kong Q, Chen X, Wu M, et al. Correlative analyses of the expression levels of PIAS3, p-SHP2, SOCS1 and SOCS3 with STAT3 activation in human astrocytomas. Mol Med Rep. 2017;15:847-852 pubmed publisher
    ..signaling (SOCS) 1 and SOCS3, SH2 domain?containing phosphatase (SHP2) and protein inhibitors of activated STAT3 (PIAS3) are known to inhibit STAT3 signal transduction, while their expression statuses in the four grades of ..
  8. Gao W, McGarry T, Orr C, McCormick J, Veale D, Fearon U. Tofacitinib regulates synovial inflammation in psoriatic arthritis, inhibiting STAT activation and induction of negative feedback inhibitors. Ann Rheum Dis. 2016;75:311-5 pubmed publisher
    ..pSTAT3), phosphoSTAT1 (pSTAT1), suppressor of cytokine signaling-3 (SOCS3), protein inhibitor of activated Stat3 (PIAS3) and nuclear factor kappa B cells (NFκBp65) were quantified by western blot...
  9. Tang X, Yin K, Zhu H, Tian J, Shen D, Yi L, et al. Correlation Between the Expression of MicroRNA-301a-3p and the Proportion of Th17 Cells in Patients with Rheumatoid Arthritis. Inflammation. 2016;39:759-67 pubmed publisher
    ..We also observed that the expression of protein inhibitor of activated STAT3 (PIAS3), the main cellular inhibitor of STAT3, was attenuated in RA patients and negatively correlated with the ..
  10. Miller A, Levy C, Davis I, Razin E, Fisher D. Sumoylation of MITF and its related family members TFE3 and TFEB. J Biol Chem. 2005;280:146-55 pubmed
    ..Sumoylation, thus, provides a possible mechanism for altering the effects of MITF by affecting the target genes that it activates. ..
  11. Zhao Y, Heerman M, Peng W, Evans J, Rose R, Degrandi Hoffman G, et al. The Dynamics of Deformed Wing Virus Concentration and Host Defensive Gene Expression after Varroa Mite Parasitism in Honey Bees, Apis mellifera. Insects. 2019;10: pubmed publisher
    ..corresponding immune effector molecules, and the small ubiquitin-like modifier (SUMO) ligase repressor of Dorsal, PIAS3. We observed a similar expression pattern for genes expressing transcripts for the RNA interference (Dicer/..
  12. Park J, Lee S, Yang S, Yoo H, Park J, Seong M, et al. Modification of DBC1 by SUMO2/3 is crucial for p53-mediated apoptosis in response to DNA damage. Nat Commun. 2014;5:5483 pubmed publisher
    ..Whereas etoposide treatment reduced the interaction of DBC1 with SENP1, it promoted that with PIAS3, resulting in an increase in DBC1 sumoylation...
  13. Higginbotham J, O Shea C. Adenovirus E4-ORF3 Targets PIAS3 and Together with E1B-55K Remodels SUMO Interactions in the Nucleus and at Virus Genome Replication Domains. J Virol. 2015;89:10260-72 pubmed publisher
    ..We show that E4-ORF3 specifically targets and sequesters the cellular E3 SUMO ligase PIAS3 but not PIAS1, PIAS2, or PIAS4...
  14. ANDRADE D, Velinder M, Singer J, Maese L, Bareyan D, Nguyen H, et al. SUMOylation Regulates Growth Factor Independence 1 in Transcriptional Control and Hematopoiesis. Mol Cell Biol. 2016;36:1438-50 pubmed publisher
    ..We show a binding interface between the GFI1 linker and the SP-RING domain of PIAS3, an E3-SUMO (small ubiquitin-related modifier) ligase...
  15. Min H, Choi J, Lee S, Seo H, Jung K, Na H, et al. Protein inhibitor of activated STAT3 reduces peripheral arthritis and gut inflammation and regulates the Th17/Treg cell imbalance via STAT3 signaling in a mouse model of spondyloarthritis. J Transl Med. 2019;17:18 pubmed publisher
    ..This study investigated the effects of protein inhibitor of activated STAT3 (PIAS3) on SpA pathogenesis. Curdlan was injected into SKG ZAP-70W163C mice for SpA induction...
  16. Dütting E, Schröder Kress N, Sticht H, Enz R. SUMO E3 ligases are expressed in the retina and regulate SUMOylation of the metabotropic glutamate receptor 8b. Biochem J. 2011;435:365-71 pubmed publisher
    ..In summary, the results of the present study show in vivo SUMOylation of the complete mGluR8b and co-localize proteins of the SUMOylation machinery in the retina. ..
  17. Dalmasso G, Nguyen H, Faïs T, Massier S, Barnich N, Delmas J, et al. Crohn's Disease-Associated Adherent-Invasive Escherichia coli Manipulate Host Autophagy by Impairing SUMOylation. Cells. 2019;8: pubmed publisher
    ..LF82-induced deSUMOylation in IECs was due in part to increased level of microRNA (miR)-18, which targets PIAS3 mRNA encoding a protein involved in SUMOylation...
  18. Lemos T, Kobarg J. CGI-55 interacts with nuclear proteins and co-localizes to p80-coilin positive-coiled bodies in the nucleus. Cell Biochem Biophys. 2006;44:463-74 pubmed
    ..However, we observed that CGI-55 localizes to the nucleolus and co-localizes with p80-coilin positive nuclear-coiled bodies...
  19. Taheri M, Azimi G, Sayad A, Mazdeh M, Arsang Jang S, Omrani M, et al. Expression analysis of protein inhibitor of activated STAT (PIAS) genes in IFNβ-treated multiple sclerosis patients. J Inflamm Res. 2018;11:457-463 pubmed publisher
    ..i>PIAS2 and PIAS3 expressions were inversely correlated with Expanded Disability Status Scale (EDSS) in patients...
  20. Josa Prado F, Henley J, Wilkinson K. SUMOylation of Argonaute-2 regulates RNA interference activity. Biochem Biophys Res Commun. 2015;464:1066-1071 pubmed publisher
    ..We used the putative substrate recognition (PINIT) domain from the SUMO E3 PIAS3 as bait to identify potential SUMO substrates...
  21. Pan C, Wang M, Chiou S, Chen H, Wu C. Oncostatin M suppresses metastasis of lung adenocarcinoma by inhibiting SLUG expression through coordination of STATs and PIASs signalings. Oncotarget. 2016;7:60395-60406 pubmed publisher
    ..In contrast, PIAS3 bound to activated STAT3, another effector of OSM, in response to OSM and blocked the binding of STAT3 to SLUG ..
  22. Song X, Shu X, Wu M, Zheng X, Jia B, Kong Q, et al. Postoperative resveratrol administration improves prognosis of rat orthotopic glioblastomas. BMC Cancer. 2018;18:871 pubmed publisher
    ..resveratrol-treated tumour tissues showed less Ki67 labelling, widely distributed apoptotic regions, upregulated PIAS3 expression and reduced p-STAT3 nuclear translocation...
  23. Zhang C, Mukherjee S, Tucker Burden C, Ross J, Chau M, Kong J, et al. TRIM8 regulates stemness in glioblastoma through PIAS3-STAT3. Mol Oncol. 2017;11:280-294 pubmed publisher
    ..We found that TRIM8 activates STAT3 by suppressing the expression of PIAS3, an inhibitor of STAT3, most likely through E3-mediated ubiquitination and proteasomal degradation...
  24. Wang L, Zhang J, Banerjee S, Barnes L, Sajja V, Liu Y, et al. Sumoylation of vimentin354 is associated with PIAS3 inhibition of glioma cell migration. Oncotarget. 2010;1:620-7 pubmed
    ..Over-expression of PIAS3 effectively changes cell shape and inhibits GBM cell migration...
  25. Nakagawa K, Yokosawa H. PIAS3 induces SUMO-1 modification and transcriptional repression of IRF-1. FEBS Lett. 2002;530:204-8 pubmed
    ..the function of IRF-1, we performed yeast two-hybrid screening and isolated protein inhibitor of activated STAT3 (PIAS3) as an IRF-1-binding protein...
  26. Sun L, Li H, Chen J, Dehennaut V, Zhao Y, Yang Y, et al. A SUMOylation-dependent pathway regulates SIRT1 transcription and lung cancer metastasis. J Natl Cancer Inst. 2013;105:887-98 pubmed publisher
    ..We have identified a novel pathway that links SIRT1 down-regulation to hypoxia-induced EMT in lung cancer cells and may shed light on the development of novel antitumor therapeutics. ..
  27. Dai X, Ahn K, Kim C, Siveen K, Ong T, Shanmugam M, et al. Ascochlorin, an isoprenoid antibiotic inhibits growth and invasion of hepatocellular carcinoma by targeting STAT3 signaling cascade through the induction of PIAS3. Mol Oncol. 2015;9:818-33 pubmed publisher
    ..ASC increased the expression of protein inhibitor of activated STAT3 (PIAS3) which could bind to STAT3 DNA binding domain and thereby down-regulate STAT3 activation...
  28. Pastuschek J, Poetzsch J, Morales Prieto D, Schleußner E, Markert U, Georgiev G. Stimulation of the JAK/STAT pathway by LIF and OSM in the human granulosa cell line COV434. J Reprod Immunol. 2015;108:48-55 pubmed publisher
    ..Expression of GP130 (glycoprotein 130), STAT3 (signal transducer and activators of transcription 3), PIAS3 (protein inhibitor of activated STAT 3), and SOCS3 (suppressor of cytokine signaling 3) genes after stimulation ..
  29. Napimoga M, Demasi A, Jarry C, Ortega M, de Araújo V, Martinez E. In vitro evaluation of the biological effect of SOFAT on osteoblasts. Int Immunopharmacol. 2015;26:378-83 pubmed publisher
    ..pathway demonstrated that SOFAT induced the expression of BCL2, IL1B, IL10, IL22, IL2RA, IL4, IL6, TNFSF10 and PIAS3, while IL2, IL21, CD4, CSF3R and TNF were repressed...
  30. Usui N, Co M, Harper M, Rieger M, Dougherty J, Konopka G. Sumoylation of FOXP2 Regulates Motor Function and Vocal Communication Through Purkinje Cell Development. Biol Psychiatry. 2017;81:220-230 pubmed publisher
    ..In vitro co-immunoprecipitation and in vivo colocalization experiments suggest that PIAS3 acts as the small ubiquitin-like modifier E3 ligase for FOXP2 sumoylation...
  31. Kotaja N, Aittomaki S, Silvennoinen O, Palvimo J, Janne O. ARIP3 (androgen receptor-interacting protein 3) and other PIAS (protein inhibitor of activated STAT) proteins differ in their ability to modulate steroid receptor-dependent transcriptional activation. Mol Endocrinol. 2000;14:1986-2000 pubmed
    ..analyzed herein the roles that four different PIAS proteins (ARIP3/PIASx alpha, Miz1/PIASx beta, GBP/PIAS1, and PIAS3) play in the regulation of steroid receptor- or STAT-mediated transcriptional activation...
  32. Ko J, Ho Baek S, Nam D, Chung W, Lee S, Lee J, et al. 3-Formylchromone inhibits proliferation and induces apoptosis of multiple myeloma cells by abrogating STAT3 signaling through the induction of PIAS3. Immunopharmacol Immunotoxicol. 2016;38:334-43 pubmed publisher
    ..Furthermore, 3FC induced the expression of the protein inhibitors of activated STAT3 (PIAS3), and gene silencing of the PIAS3 by small interfering RNA abolished the ability of 3FC to inhibit STAT3 ..
  33. Siatecka M, Soni S, Planutis A, Bieker J. Transcriptional activity of erythroid Kruppel-like factor (EKLF/KLF1) modulated by PIAS3 (protein inhibitor of activated STAT3). J Biol Chem. 2015;290:9929-40 pubmed publisher
    ..Our studies identify PIAS3 as a transcriptional corepressor of EKLF for at least a subset of its target genes during erythropoiesis (e.g...
  34. Saitoh M, Endo K, Furuya S, Minami M, Fukasawa A, Imamura T, et al. STAT3 integrates cooperative Ras and TGF-β signals that induce Snail expression. Oncogene. 2016;35:1049-57 pubmed publisher
    ..Protein inhibitor of activated STAT3 (PIAS3) inhibited the enhanced Snail promoter activity induced by TGF-β and Ras...
  35. Murakami H, Arnheiter H. Sumoylation modulates transcriptional activity of MITF in a promoter-specific manner. Pigment Cell Res. 2005;18:265-77 pubmed
    ..The results suggest that sumoylation plays a significant role among the multiple mechanisms that regulate MITF during development and in adulthood. ..
  36. Abbas R, McColl K, Kresak A, Yang M, Chen Y, Fu P, et al. PIAS3 expression in squamous cell lung cancer is low and predicts overall survival. Cancer Med. 2015;4:325-32 pubmed publisher
    ..report we examine the status of an endogenous inhibitor of STAT3, called Protein Inhibitor of Activated STAT3 (PIAS3), in SCC and its potential role in this disease...
  37. Li J, Cui J, Zhang J, Liu Y, Han L, Jia C, et al. PIAS3, an inhibitor of STAT3, has intensively negative association with the survival of gastric cancer. Int J Clin Exp Med. 2015;8:682-9 pubmed
    ..value of the expression of the protein inhibitor of activated signal transducer and activators of transcription 3 (PIAS3) in gastric cancer (GC)...
  38. Tsuruma R, Ohbayashi N, Kamitani S, Ikeda O, Sato N, Muromoto R, et al. Physical and functional interactions between STAT3 and KAP1. Oncogene. 2008;27:3054-9 pubmed
    ..These results indicate that KAP1 may serve as a transcriptional regulator of the IL-6/STAT3 signaling pathway. ..
  39. Campia I, Buondonno I, Castella B, Rolando B, Kopecka J, Gazzano E, et al. An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells. PLoS ONE. 2015;10:e0126159 pubmed publisher
    ..In chemoresistant cells the basal activity of JAK1/STAT1 and JAK1/STAT3 signaling was higher, the STAT3 inhibitor PIAS3 was down-regulated, and the autocrine production of STAT3-target and IDO1-inducers cytokines IL-6, IL-4, IL-1β, ..
  40. Jiao J, Zhang R, Li Z, Yin Y, Fang X, Ding X, et al. Nuclear Smad6 promotes gliomagenesis by negatively regulating PIAS3-mediated STAT3 inhibition. Nat Commun. 2018;9:2504 pubmed publisher
    ..patient survival and regulates STAT3 activity via negatively regulating the Protein Inhibitors of Activated STAT3 (PIAS3)...
  41. Takahashi K, Taira T, Niki T, Seino C, Iguchi Ariga S, Ariga H. DJ-1 positively regulates the androgen receptor by impairing the binding of PIASx alpha to the receptor. J Biol Chem. 2001;276:37556-63 pubmed
    ..These results indicate that DJ-1 is a positive regulator of the androgen receptor. ..
  42. Kumar R, González Prieto R, Xiao Z, Verlaan de Vries M, Vertegaal A. The STUbL RNF4 regulates protein group SUMOylation by targeting the SUMO conjugation machinery. Nat Commun. 2017;8:1809 pubmed publisher
    ..Here we report that the single SUMO E2 Ubc9 and the SUMO E3 ligases PIAS1, PIAS2, PIAS3, ZNF451, and NSMCE2 are direct RNF4 targets. We confirm PIAS1 as a key RNF4 substrate...
  43. Depaux A, Regnier Ricard F, Germani A, Varin Blank N. A crosstalk between hSiah2 and Pias E3-ligases modulates Pias-dependent activation. Oncogene. 2007;26:6665-76 pubmed
    ..This report describes a new interconnection between sumoylation and ubiquitination pathways by regulating the levels of the E3-ligases available for these processes. ..
  44. Jambunathan S, Fontes J. Sumoylation of the zinc finger protein ZXDC enhances the function of its transcriptional activation domain. Biol Chem. 2007;388:965-72 pubmed
    ..Rather, sumoylation of ZXDC is required for full activity of the transcriptional activation domain. Our findings suggest that sumoylation is an important regulator of ZXDC. ..
  45. Liu Y, Bridges R, Wortham A, Kulesz Martin M. NF-?B repression by PIAS3 mediated RelA SUMOylation. PLoS ONE. 2012;7:e37636 pubmed publisher
    ..experimental evidence that NF-?B is regulated by SUMOylation, where the RelA subunit of NF-?B is SUMOylated by PIAS3, a member of the PIAS (protein inhibitor of activated STAT) protein family with E3 SUMO ligase activity...
  46. Wu C, Zou L. The SUMO (Small Ubiquitin-like Modifier) Ligase PIAS3 Primes ATR for Checkpoint Activation. J Biol Chem. 2016;291:279-90 pubmed publisher
    ..Here, we show that the PIAS3 SUMO ligase is important for activation of the ATR (ataxia telangiectasia and Rad3 related)-regulated DNA damage ..
  47. Tan J, Sun Y, Song J, Chen Y, Krontiris T, Durrin L. SUMO conjugation to the matrix attachment region-binding protein, special AT-rich sequence-binding protein-1 (SATB1), targets SATB1 to promyelocytic nuclear bodies where it undergoes caspase cleavage. J Biol Chem. 2008;283:18124-34 pubmed publisher
    ..The role of regulated proteolysis of SATB1 may be to control transcription in immune cells during normal cell functions or to assist in efficient and rapid clearance of nonfunctional or potentially damaging immune cells. ..
  48. Lee S, Lee M, Park J, Kang S, Yoo H, Ka S, et al. SUMOylation of hnRNP-K is required for p53-mediated cell-cycle arrest in response to DNA damage. EMBO J. 2012;31:4441-52 pubmed publisher
    ..b>PIAS3 served as a small ubiquitin-related modifier (SUMO) E3 ligase for hnRNP-K in an ATR-dependent manner...
  49. Wang X, Jiang J, Lu Y, Shi G, Liu R, Cao Y. TAB2, an important upstream adaptor of interleukin-1 signaling pathway, is subject to SUMOylation. Mol Cell Biochem. 2014;385:69-77 pubmed publisher
    ..b>PIAS3, a SUMO E3 ligase, preferentially interacted with and promoted its SUMOylation...
  50. Pitari M, Rossi M, Amodio N, Botta C, Morelli E, Federico C, et al. Inhibition of miR-21 restores RANKL/OPG ratio in multiple myeloma-derived bone marrow stromal cells and impairs the resorbing activity of mature osteoclasts. Oncotarget. 2015;6:27343-58 pubmed publisher
    ..Overexpression of protein inhibitor of activated STAT3 (PIAS3), which is a direct and validated target of miR-21, antagonized STAT3-mediated RANKL gene activation...
  51. Tirard M, Jasbinsek J, Almeida O, Michaelidis T. The manifold actions of the protein inhibitor of activated STAT proteins on the transcriptional activity of mineralocorticoid and glucocorticoid receptors in neural cells. J Mol Endocrinol. 2004;32:825-41 pubmed
    ..In neural cells, PIAS3 exhibited a strong and specific interaction with MR, but not GR, as indicated by mammalian two-hybrid assays and ..
  52. Li C, Li H, Zhang P, Yu L, Huang T, Song X, et al. SHP2, SOCS3 and PIAS3 Expression Patterns in Medulloblastomas: Relevance to STAT3 Activation and Resveratrol-Suppressed STAT3 Signaling. Nutrients. 2016;9: pubmed publisher
    Activated STAT3 signaling is critical for human medulloblastoma cells. SHP2, SOCS3 and PIAS3 are known as the negative regulators of STAT3 signaling, while their relevance to frequent STAT3 activation in medulloblastomas remains unknown...
  53. Campla C, Breit H, Dong L, Gumerson J, Roger J, Swaroop A. Pias3 is necessary for dorso-ventral patterning and visual response of retinal cones but is not required for rod photoreceptor differentiation. Biol Open. 2017;6:881-890 pubmed publisher
    Protein inhibitor of activated Stat 3 (Pias3) is implicated in guiding specification of rod and cone photoreceptors through post-translational modification of key retinal transcription factors...
  54. Saini U, Suarez A, Naidu S, Wallbillich J, Bixel K, Wanner R, et al. STAT3/PIAS3 Levels Serve as "Early Signature" Genes in the Development of High-Grade Serous Carcinoma from the Fallopian Tube. Cancer Res. 2018;78:1739-1750 pubmed publisher
    ..of transcription 3 (pSTAT3 Tyr705) and suppression or loss of protein inhibitor of activated STAT3 (PIAS3) in FT likely drive HGSC...
  55. Nery F, Rui E, Kuniyoshi T, Kobarg J. Evidence for the interaction of the regulatory protein Ki-1/57 with p53 and its interacting proteins. Biochem Biophys Res Commun. 2006;341:847-55 pubmed
    ..Furthermore, we found that the phosphorylation of p53 by PKC abolishes its interaction with Ki-1/57 in vitro. ..
  56. Zhang P, Yang B, Yao Y, Zhong L, Chen X, Kong Q, et al. PIAS3, SHP2 and SOCS3 Expression patterns in Cervical Cancers: Relevance with activation and resveratrol-caused inactivation of STAT3 signaling. Gynecol Oncol. 2015;139:529-35 pubmed publisher
    ..However, the mechanism of resveratrol-induced STAT3 inactivation remains largely unknown. SHP2, PIAS3, and SOCS3 are STAT3 negative regulators; therefore, their statuses in cervical adenocarcinoma (HeLa) and squamous ..
  57. Chin D, Sakurai M, Nah G, Du L, Jacob B, Yokomizo T, et al. RUNX1 haploinsufficiency results in granulocyte colony-stimulating factor hypersensitivity. Blood Cancer J. 2016;6:e379 pubmed publisher
    ..pronounced phosphorylation of STAT3 as compared with Runx1(+/+) cells, which may be due to reduced expression of Pias3, a key negative regulator of STAT3 signaling, and reduced physical sequestration of STAT3 by RUNX1...
  58. Huang G, Wu X, Li S, Xu X, Zhu H, Chen X. The long noncoding RNA CASC2 functions as a competing endogenous RNA by sponging miR-18a in colorectal cancer. Sci Rep. 2016;6:26524 pubmed publisher
    ..028). Further functional experiments indicate that CASC2 could directly upregulate PIAS3 expression by functioning as a competing endogenous RNA (ceRNA) for miR-18a...
  59. Rodel B, Tavassoli K, Karsunky H, Schmidt T, Bachmann M, Schaper F, et al. The zinc finger protein Gfi-1 can enhance STAT3 signaling by interacting with the STAT3 inhibitor PIAS3. EMBO J. 2000;19:5845-55 pubmed
    ..The recently discovered protein PIAS3 binds directly to STAT3 and blocks transcriptional activation...
  60. Prigge J, Schmidt E. Interaction of protein inhibitor of activated STAT (PIAS) proteins with the TATA-binding protein, TBP. J Biol Chem. 2006;281:12260-9 pubmed
    ..Two members of the protein inhibitor of activated STAT (PIAS) family, PIAS1 and PIAS3, also interacted with TBP in screens...
  61. Tan J, Song J, Chen Y, Durrin L. Phosphorylation-dependent interaction of SATB1 and PIAS1 directs SUMO-regulated caspase cleavage of SATB1. Mol Cell Biol. 2010;30:2823-36 pubmed publisher
    ..Thus, this motif may be commonly involved in interaction with the PIAS SAP, and phosphorylation may similarly inhibit some of these substrates by preventing their interaction with the ligase. ..
  62. Masotti A, Donninelli G, Da Sacco L, Varano B, Del Cornò M, Gessani S. HIV-1 gp120 influences the expression of microRNAs in human monocyte-derived dendritic cells via STAT3 activation. BMC Genomics. 2015;16:480 pubmed publisher
  63. Wang R, Zhang Y, Xu L, Lin Y, Yang X, Bai L, et al. Protein Inhibitor of Activated STAT3 Suppresses Oxidized LDL-induced Cell Responses during Atherosclerosis in Apolipoprotein E-deficient Mice. Sci Rep. 2016;6:36790 pubmed publisher
    ..Protein inhibitor of activated STAT3 (PIAS3) is the key negative regulator of JAK/STAT3 signalling. However, its effect on atherogenesis is unknown...
  64. Levy C, Lee Y, Nechushtan H, Schueler Furman O, Sonnenblick A, Hacohen S, et al. Identifying a common molecular mechanism for inhibition of MITF and STAT3 by PIAS3. Blood. 2006;107:2839-45 pubmed
    Protein inhibitor of activated STAT3 (PIAS3) functions in vivo as a key molecule in suppressing the transcriptional activity of both microphthalmia transcription factor (MITF) and signal transducer and activator of transcription 3 (STAT3)..
  65. Chandhoke A, Karve K, Dadakhujaev S, Netherton S, Deng L, Bonni S. The ubiquitin ligase Smurf2 suppresses TGFβ-induced epithelial-mesenchymal transition in a sumoylation-regulated manner. Cell Death Differ. 2016;23:876-88 pubmed publisher
    ..We find that the SUMO-E2 conjugating enzyme Ubc9 and the SUMO E3 ligase PIAS3 associate with Smurf2 and promote its sumoylation at the distinct sites of Lysines 26 and 369...
  66. Ueki N, Seki N, Yano K, Saito T, Masuho Y, Muramatsu M. Isolation and chromosomal assignment of a human gene encoding protein inhibitor of activated STAT3 (PIAS3). J Hum Genet. 1999;44:193-6 pubmed
    Mouse PIAS3 (protein inhibitor of activated STAT3) is a specific inhibitor of STAT3 that downregulates its signaling pathway. Here we report the isolation and chromosome mapping of the human PIAS3 gene...
  67. Duechting A, Tschope C, Kaiser H, Lamkemeyer T, Tanaka N, Aberle S, et al. Human parvovirus B19 NS1 protein modulates inflammatory signaling by activation of STAT3/PIAS3 in human endothelial cells. J Virol. 2008;82:7942-52 pubmed publisher
    ..However, the level of PIAS3 was upregulated in NS1-expressing HMEC-1 cells...
  68. Starkel P, De Saeger C, Leclercq I, Strain A, Horsmans Y. Deficient Stat3 DNA-binding is associated with high Pias3 expression and a positive anti-apoptotic balance in human end-stage alcoholic and hepatitis C cirrhosis. J Hepatol. 2005;43:687-95 pubmed
    ..Deficient Stat3 DNA-binding was associated with high Pias3 expression, but not with increased Socs3 levels...
  69. Sundvall M, Korhonen A, Vaparanta K, Anckar J, Halkilahti K, Salah Z, et al. Protein inhibitor of activated STAT3 (PIAS3) protein promotes SUMOylation and nuclear sequestration of the intracellular domain of ErbB4 protein. J Biol Chem. 2012;287:23216-26 pubmed publisher
    ..In search of regulatory mechanisms of ErbB4 ICD function, we identified PIAS3 as a novel interaction partner of ErbB4 ICD...
  70. Kabil O, Zhou Y, Banerjee R. Human cystathionine beta-synthase is a target for sumoylation. Biochemistry. 2006;45:13528-36 pubmed
    ..that interact with CBS and have identified several components of the sumoylation pathway including Ubc9, PIAS1, PIAS3, Pc2, and RanBPM...
  71. Dabir S, Kluge A, Kresak A, Yang M, Fu P, Groner B, et al. Low PIAS3 expression in malignant mesothelioma is associated with increased STAT3 activation and poor patient survival. Clin Cancer Res. 2014;20:5124-32 pubmed publisher
    ..We examined the extent of PIAS3 expression in mesothelioma cells and human tumor samples and determined the functional effects of PIAS3 expression ..
  72. Yoo J, Jeong J, Fazleabas A, Tayade C, Young S, Lessey B. Protein Inhibitor of Activated STAT3 (PIAS3) Is Down-Regulated in Eutopic Endometrium of Women with Endometriosis. Biol Reprod. 2016;95:11 pubmed publisher
    ..Protein inhibitor of activated STAT3 (PIAS3) is a negative regulator of STAT3 activity...
  73. Knittle A, Helkkula M, Johnson M, Sundvall M, Elenius K. SUMOylation regulates nuclear accumulation and signaling activity of the soluble intracellular domain of the ErbB4 receptor tyrosine kinase. J Biol Chem. 2017;292:19890-19904 pubmed publisher
    ..is posttranslationally modified by the small ubiquitin-like modifier (SUMO) and functionally interacts with the PIAS3 SUMO E3 ligase. However, direct evidence of SUMO modification in ErbB4 signaling has remained elusive...
  74. Wang L, Yang X, Mihalic K, Xiao W, Li D, Farrar W. Activation of estrogen receptor blocks interleukin-6-inducible cell growth of human multiple myeloma involving molecular cross-talk between estrogen receptor and STAT3 mediated by co-regulator PIAS3. J Biol Chem. 2001;276:31839-44 pubmed
    ..Estrogen-activated ER did not associate directly with STAT3. Estrogen induced the mRNA expression of PIAS3 (protein inhibitor of activated STAT3) and increased PIAS3 physical association with STAT3, suggesting a possible ..
  75. Cheng J, Zhang D, Zhou C, Marasco W. Down-regulation of SHP1 and up-regulation of negative regulators of JAK/STAT signaling in HTLV-1 transformed cell lines and freshly transformed human peripheral blood CD4+ T-cells. Leuk Res. 2004;28:71-82 pubmed
    ..signaling-1 (SOCS1) and protein inhibitor of activated signal transducer and activator of transcription 3 (STAT3) (PIAS3) in six HTLV-1 negative and seven HTLV-1 positive T-cell leukemia lines...
  76. Dünnebier T, Bermejo J, Haas S, Fischer H, Pierl C, Justenhoven C, et al. Polymorphisms in the UBC9 and PIAS3 genes of the SUMO-conjugating system and breast cancer risk. Breast Cancer Res Treat. 2010;121:185-94 pubmed publisher
    ..for detection of putative functional polymorphisms in 14 genes of the SUMO system, we selected one coding SNP in PIAS3 and seven tag SNPs in UBC9 for association analyses...
  77. Zhang D, Lu C, Yu Z, Wang X, Yan L, Zhang J, et al. Echinacoside Alleviates UVB Irradiation-Mediated Skin Damage via Inhibition of Oxidative Stress, DNA Damage, and Apoptosis. Oxid Med Cell Longev. 2017;2017:6851464 pubmed publisher
    ..oxidative stress, DNA damage, and apoptosis caused by UVB exposure; and normalized the protein levels of ATR, p53, PIAS3, hnRNP K, PARP, and XPA...
  78. Zentner M, Lin H, Deng H, Kim K, Shih H, Ann D. Requirement for high mobility group protein HMGI-C interaction with STAT3 inhibitor PIAS3 in repression of alpha-subunit of epithelial Na+ channel (alpha-ENaC) transcription by Ras activation in salivary epithelial cells. J Biol Chem. 2001;276:29805-14 pubmed
    ..and co-immunoprecipitation assays from eukaryotic cells, we also discovered the interaction between HMGI-C and PIAS3 (protein inhibitor of activated STAT3 (signal transducer and activator of transcription 3))...
  79. Lamoliatte F, Caron D, Durette C, Mahrouche L, Maroui M, Caron Lizotte O, et al. Large-scale analysis of lysine SUMOylation by SUMO remnant immunoaffinity profiling. Nat Commun. 2014;5:5409 pubmed publisher
    ..In contrast, a CDC73 K136R mutant translocates to the cytoplasm under the same conditions, further demonstrating the effectiveness of our method to characterize the dynamics of lysine SUMOylation. ..
  80. Stehmeier P, Muller S. Phospho-regulated SUMO interaction modules connect the SUMO system to CK2 signaling. Mol Cell. 2009;33:400-9 pubmed publisher
    ..CK2-regulated phosphoSIM modules were also dissected in the tumor suppressor PML and the exosome component PMSCL1, indicating that these modules serve as general platforms that integrate CK2- and SUMO-regulated signaling networks. ..
  81. Castillo Lluva S, Tatham M, Jones R, Jaffray E, Edmondson R, Hay R, et al. SUMOylation of the GTPase Rac1 is required for optimal cell migration. Nat Cell Biol. 2010;12:1078-85 pubmed publisher
    ..Here, we identify that the small ubiquitin-like modifier (SUMO) E3-ligase, PIAS3, interacts with Rac1 and is required for increased Rac activation and optimal cell migration in response to ..
  82. Levy C, Nechushtan H, Razin E. A new role for the STAT3 inhibitor, PIAS3: a repressor of microphthalmia transcription factor. J Biol Chem. 2002;277:1962-6 pubmed
    ..MITF gene as the bait in the screening of a mast cell library, resulted in the isolation of the STAT3 inhibitor, PIAS3. PIAS3 is a transcriptional inhibitor that acts by specifically inhibiting STAT3's DNA binding activity...
  83. Jimenez Lara A, Heine M, Gronemeyer H. PIAS3 (protein inhibitor of activated STAT-3) modulates the transcriptional activation mediated by the nuclear receptor coactivator TIF2. FEBS Lett. 2002;526:142-6 pubmed
    b>PIAS3, a member of the protein inhibitor of activated STAT family, was found to interact in vivo and in vitro with TIF2, a previously described coactivator for nuclear receptors...
  84. Yang S, Hou M, Chen F, Ou Yang F, Wu Y, Chai C, et al. Prognostic value of protein inhibitor of activated STAT3 in breast cancer patients receiving hormone therapy. BMC Cancer. 2016;16:20 pubmed publisher
    ..Alterations in specific negative regulators, such as protein inhibitor of activated STAT3 (PIAS3), may contribute to cancer development...
  85. Liu B, Liao J, Rao X, Kushner S, Chung C, Chang D, et al. Inhibition of Stat1-mediated gene activation by PIAS1. Proc Natl Acad Sci U S A. 1998;95:10626-31 pubmed
    ..These results identify PIAS1 as a specific inhibitor of Stat1-mediated gene activation and suggest that there may exist a specific PIAS inhibitor in every STAT signaling pathway. ..
  86. Di Y, Li J, Zhang Y, He X, Lu H, Xu D, et al. HCC-associated protein HCAP1, a variant of GEMIN4, interacts with zinc-finger proteins. J Biochem. 2003;133:713-8 pubmed
    ..By deletion analysis, the leucine zipper domain of HCAP1 and the zinc finger domain of NDP52 were identified as important regions responsible for the interaction. ..
  87. Yamamoto T, Sato N, Sekine Y, Yumioka T, Imoto S, Junicho A, et al. Molecular interactions between STAT3 and protein inhibitor of activated STAT3, and androgen receptor. Biochem Biophys Res Commun. 2003;306:610-5 pubmed
    ..The recently discovered protein inhibitor of activated STAT3 (PIAS3) binds directly to STAT3 and blocks transcriptional activation...
  88. Okumura F, Matsunaga Y, Katayama Y, Nakayama K, Hatakeyama S. TRIM8 modulates STAT3 activity through negative regulation of PIAS3. J Cell Sci. 2010;123:2238-45 pubmed publisher
    ..Here, we show that TRIM8 interacts with protein inhibitor of activated STAT3 (PIAS3), which inhibits IL-6-dependent activation of STAT3...
  89. Wu W, Takanashi M, Borjigin N, Ohno S, Fujita K, Hoshino S, et al. MicroRNA-18a modulates STAT3 activity through negative regulation of PIAS3 during gastric adenocarcinogenesis. Br J Cancer. 2013;108:653-61 pubmed publisher
    ..In addition, we identified Protein Inhibitor of Activated Signal Transducer and Activator of Transcription 3 (PIAS3) as a direct target of miR-18a in gastric cancer...
  90. Lao M, Shi M, Zou Y, Huang M, Ye Y, Qiu Q, et al. Protein Inhibitor of Activated STAT3 Regulates Migration, Invasion, and Activation of Fibroblast-like Synoviocytes in Rheumatoid Arthritis. J Immunol. 2016;196:596-606 pubmed publisher
    ..Protein inhibitor of activated STAT (PIAS), whose family members include PIAS1, PIAS2 (PIASx), PIAS3, and PIAS4 (PIASy), play important roles in regulating various cellular events, such as cell survival, migration, ..
  91. Spoden G, Morandell D, Ehehalt D, Fiedler M, Jansen Durr P, Hermann M, et al. The SUMO-E3 ligase PIAS3 targets pyruvate kinase M2. J Cell Biochem. 2009;107:293-302 pubmed publisher
    ..Here we show that the SUMO-E3 ligase protein PIAS3 (inhibitor of activated STAT3) physically interacts with M2-PK and its isoenzyme M1-PK...
  92. Sun X, Li J, Dong F, Dong J. Characterization of nuclear localization and SUMOylation of the ATBF1 transcription factor in epithelial cells. PLoS ONE. 2014;9:e92746 pubmed publisher
    ..Finally, the PIAS3 SUMO1 E3 ligase, which interacts with ATBF1 directly, diminished rather than enhanced ATBF1 SUMOylation, ..