Gene Symbol: PHPT1
Description: phosphohistidine phosphatase 1
Alias: CGI-202, HEL-S-132P, HSPC141, PHP, PHP14, 14 kDa phosphohistidine phosphatase, epididymis secretory sperm binding protein Li 132P, protein histidine phosphatase, protein janus-A homolog, sex-regulated protein janus-a
Species: human
Products:     PHPT1

Top Publications

  1. Srivastava S, Zhdanova O, Di L, Li Z, Albaqumi M, Wulff H, et al. Protein histidine phosphatase 1 negatively regulates CD4 T cells by inhibiting the K+ channel KCa3.1. Proc Natl Acad Sci U S A. 2008;105:14442-6 pubmed publisher
    ..1 channel activation in human CD4 T lymphocytes. We now show that the mammalian protein histidine phosphatase (PHPT-1) directly binds and inhibits KCa3.1 by dephosphorylating histidine 358 on KCa3.1...
  2. Cruz Garcia L, O Brien G, Donovan E, Gothard L, Boyle S, Laval A, et al. Influence of Confounding Factors on Radiation Dose Estimation Using In Vivo Validated Transcriptional Biomarkers. Health Phys. 2018;115:90-101 pubmed publisher
    ..Their potential influence on the transcriptional response to radiation of the genes CCNG1 and PHPT1, two biomarkers of radiation exposure ex vivo, was assessed...
  3. Reddy P, Dery S, Metanis N. Chemical Synthesis of Proteins with Non-Strategically Placed Cysteines Using Selenazolidine and Selective Deselenization. Angew Chem Int Ed Engl. 2016;55:992-5 pubmed publisher
    ..The challenging protein, 125-residue human phosphohistidine phosphatase 1 (PHPT1), has three cysteines near the C-terminus, which are not strategically placed for ligation...
  4. Fuhs S, Hunter T. pHisphorylation: the emergence of histidine phosphorylation as a reversible regulatory modification. Curr Opin Cell Biol. 2017;45:8-16 pubmed publisher
    ..Two mammalian histidine kinases (NME1 and NME2), two pHis phosphatases (PHPT1 and LHPP), and a handful of substrates were previously identified...
  5. Snezhkina A, Krasnov G, Zaretsky A, Zhavoronkov A, Nyushko K, Moskalev A, et al. Differential expression of alternatively spliced transcripts related to energy metabolism in colorectal cancer. BMC Genomics. 2016;17:1011 pubmed publisher
    ..These eight isoforms encoded by OGDH, COL6A3, ICAM1, PHPT1, PPP2R5D, SLC29A1, and TRIB3 genes were up-regulated in colorectal tumors, and this is in concordance with the ..
  6. Ma R, Kanders E, Sundh U, Geng M, Ek P, Zetterqvist O, et al. Mutational study of human phosphohistidine phosphatase: effect on enzymatic activity. Biochem Biophys Res Commun. 2005;337:887-91 pubmed
    ..Recently, a few reports have appeared on a mammalian 14-kDa phosphohistidine phosphatase, also named protein histidine phosphatase. Molecular cloning of the protein has opened possibilities for exploring its properties and ..
  7. Martin D, Dutta P, Mahajan S, Varma S, Stevens S. Structural and activity characterization of human PHPT1 after oxidative modification. Sci Rep. 2016;6:23658 pubmed publisher
    Phosphohistidine phosphatase 1 (PHPT1), the only known phosphohistidine phosphatase in mammals, regulates phosphohistidine levels of several proteins including those involved in signaling, lipid metabolism, and potassium ion transport...
  8. Cai X, Srivastava S, Surindran S, Li Z, Skolnik E. Regulation of the epithelial Ca²? channel TRPV5 by reversible histidine phosphorylation mediated by NDPK-B and PHPT1. Mol Biol Cell. 2014;25:1244-50 pubmed publisher
    ..In addition, the histidine phosphatase, protein histidine phosphatase 1, inhibits NDPK-B-activated TRPV5 in inside/out patch experiments...
  9. Seeger A, Rose K, Ma N, Kremmer E, Klumpp S, Krieglstein J. Influence of protein histidine phosphatase overexpression and down-regulation on human umbilical-vein endothelial cell viability. Cell Biol Int. 2012;36:245-9 pubmed publisher
    PHP (protein histidine phosphatase) is expressed by mammalian tissues, particularly in blood vessel walls. We investigated whether PHP plays a significant role in endothelial cells...

More Information


  1. Lai C, Chiu J, Lin W. Identification of the human crooked neck gene by comparative gene identification. Biochim Biophys Acta. 2001;1517:449-54 pubmed
    ..The unique features of the 16 copies of the tetratrico peptide repeat (TPR) motif were conserved in the yeast, fly and human crooked neck orthologous proteins, which were important for spliceosome assembly in cells. ..
  2. Kamath V, Kyathanahalli C, Jayaram B, Syed I, Olson L, Ludwig K, et al. Regulation of glucose- and mitochondrial fuel-induced insulin secretion by a cytosolic protein histidine phosphatase in pancreatic beta-cells. Am J Physiol Endocrinol Metab. 2010;299:E276-86 pubmed publisher
    We report localization of a cytosolic protein histidine phosphatase (PHP; approximately 16 kDa) in INS 832/13 cells, normal rat islets, and human islets...
  3. Gong W, Li Y, Cui G, Hu J, Fang H, Jin C, et al. Solution structure and catalytic mechanism of human protein histidine phosphatase 1. Biochem J. 2009;418:337-44 pubmed publisher
    ..However, knowledge about eukaryotic PHPT (protein histidine phosphatase) is still very limited...
  4. Inturi R, Wäneskog M, Vlachakis D, Ali Y, Ek P, Punga T, et al. A splice variant of the human phosphohistidine phosphatase 1 (PHPT1) is degraded by the proteasome. Int J Biochem Cell Biol. 2014;57:69-75 pubmed publisher
    ..Nevertheless, there is a 14 kDa enzyme with phosphohistidine phosphatase activity, named PHPT1, found in most animals, but not in bacteria, plant or fungi...
  5. Ek P, Pettersson G, Ek B, Gong F, Li J, Zetterqvist O. Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase. Eur J Biochem. 2002;269:5016-23 pubmed
    ..These results provide a new tool for studying eukaryotic histidine phosphorylation/dephosphorylation. ..
  6. Xu A, Xia X, DU S, Gu J. Clinical significance of PHPT1 protein expression in lung cancer. Chin Med J (Engl). 2010;123:3247-51 pubmed
    In our previous studies, we found the expression of 14-kD phosphohistidine phosphatase (PHPT1) was associated with lung cancer cells migration and invasion, and PHPT1 mRNA expression level in lung cancer tissues clinically correlated ..
  7. Shen H, Yang P, Liu Q, Tian Y. Nuclear expression and clinical significance of phosphohistidine phosphatase 1 in clear-cell renal cell carcinoma. J Int Med Res. 2015;43:747-57 pubmed publisher
    ..High levels of 14 kDa phosphohistidine phosphatase protein were negatively associated with overall survival and progression-free survival in patients ..
  8. Reni M, Sartori N, Mambrini A, Berardi R, Passardi A, Milella M, et al. An Italian study on treatment trends and outcomes of patients with stage III pancreatic adenocarcinoma in the gemcitabine era: is it time to change?. Anticancer Drugs. 2010;21:459-64 pubmed publisher
  9. Ek P, Ek B, Zetterqvist Ã. Phosphohistidine phosphatase 1 (PHPT1) also dephosphorylates phospholysine of chemically phosphorylated histone H1 and polylysine. Ups J Med Sci. 2015;120:20-7 pubmed publisher
    Phosphohistidine phosphatase 1 (PHPT1), also named protein histidine phosphatase (PHP), is a eukaryotic enzyme dephosphorylating proteins and peptides that are phosphorylated on a histidine residue...
  10. Xu A, Hao J, Zhang Z, Tian T, Jiang S, Hao J, et al. 14-kDa phosphohistidine phosphatase and its role in human lung cancer cell migration and invasion. Lung Cancer. 2010;67:48-56 pubmed publisher
    14-kDa phosphohistidine phosphatase (PHP14) was the first protein histidine phosphatase to be discovered, but its biological function remains unclear. In our previous study, we found that it was associated with tumor invasion...
  11. Klumpp S, Faber D, Fischer D, Litterscheid S, Krieglstein J. Role of protein histidine phosphatase for viability of neuronal cells. Brain Res. 2009;1264:7-12 pubmed publisher
    We recently found protein histidine phosphatase (PHP) in eukaryotes and identified ATP-citrate lyase (ACL) and the beta-subunit of G-proteins as its substrates...
  12. Krieglstein J, Lehmann M, Mäurer A, Gudermann T, Pinkenburg O, Wieland T, et al. Reduced viability of neuronal cells after overexpression of protein histidine phosphatase. Neurochem Int. 2008;53:132-6 pubmed publisher
    b>Protein histidine phosphatase (PHP) has just recently been discovered in eukaryotes and ATP-citrate lyase (ACL) was shown to be one of its substrates...
  13. Xu A, Li Y, Zhao W, Hou F, Li X, Sun L, et al. PHP14 regulates hepatic stellate cells migration in liver fibrosis via mediating TGF-?1 signaling to PI3K?/AKT/Rac1 pathway. J Mol Med (Berl). 2018;96:119-133 pubmed publisher
    ..We have previously demonstrated that 14 kDa phosphohistidine phosphatase (PHP14) is involved in migration and lamellipodia formation of HSCs...