PHAX

Summary

Gene Symbol: PHAX
Description: phosphorylated adaptor for RNA export
Alias: RNUXA, phosphorylated adapter RNA export protein, RNA U small nuclear RNA export adapter protein, RNA U, small nuclear RNA export adapter (phosphorylation regulated), RNA U, small nuclear RNA export adaptor (phosphorylation regulated)
Species: human
Products:     PHAX

Top Publications

  1. Schulze W, Stein F, Rettel M, Nanao M, Cusack S. Structural analysis of human ARS2 as a platform for co-transcriptional RNA sorting. Nat Commun. 2018;9:1701 pubmed publisher
    ..binding sites on ARS2 and that CBC-ARS2-NCBP3 form a ternary complex that is mutually exclusive with CBC-ARS-PHAX (involved in snRNA export)...
  2. Ansari M, Rainger J, Murray J, Hanson I, Firth H, Mehendale F, et al. A syndromic form of Pierre Robin sequence is caused by 5q23 deletions encompassing FBN2 and PHAX. Eur J Med Genet. 2014;57:587-95 pubmed publisher
    ..genes within the PRS critical region in embryonic mice showed that the strongest candidate genes were FBN2 and PHAX. Targeted aCGH of the critical region and sequencing of these genes in a cohort of 25 PRS patients revealed no ..
  3. Garcia E, Wen Y, Praveen K, Matera A. Transcriptomic comparison of Drosophila snRNP biogenesis mutants reveals mutant-specific changes in pre-mRNA processing: implications for spinal muscular atrophy. RNA. 2016;22:1215-27 pubmed publisher
    ..Here, we used disruptions in Smn and two additional snRNP biogenesis genes, Phax and Ars2, to classify RNA processing differences as snRNP-dependent or gene-specific in Drosophila Phax and Smn ..
  4. Tumlirsch T, Sznajder A, Jendrossek D. Formation of polyphosphate by polyphosphate kinases and its relationship to poly(3-hydroxybutyrate) accumulation in Ralstonia eutropha strain H16. Appl Environ Microbiol. 2015;81:8277-93 pubmed publisher
    A protein (PhaX) that interacted with poly(3-hydroxybutyrate) (PHB) depolymerase PhaZa1 and with PHB granule-associated phasin protein PhaP2 was identified by two-hybrid analysis...
  5. Giacometti S, Benbahouche N, Domanski M, Robert M, Meola N, Lubas M, et al. Mutually Exclusive CBC-Containing Complexes Contribute to RNA Fate. Cell Rep. 2017;18:2635-2650 pubmed publisher
    ..termination of several transcript types, ZC3H18 stimulates degradation of a diverse set of RNAs, and PHAX functions in pre-small nuclear RNA/small nucleolar RNA (pre-snRNA/snoRNA) transport...
  6. Ohno M, Segref A, Bachi A, Wilm M, Mattaj I. PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation. Cell. 2000;101:187-98 pubmed
    ..These components are however insufficient to support U snRNA export. We identify PHAX (phosphorylated adaptor for RNA export) as the additional factor required for U snRNA export complex assembly in ..
  7. Segref A, Mattaj I, Ohno M. The evolutionarily conserved region of the U snRNA export mediator PHAX is a novel RNA-binding domain that is essential for U snRNA export. RNA. 2001;7:351-60 pubmed
    ..the leucine-rich nuclear export signal receptor CRM1/Xpo1, RanGTP, and the recently identified phosphoprotein PHAX (phosphorylated adaptor for RNA export)...
  8. Yong J, Wan L, Dreyfuss G. Why do cells need an assembly machine for RNA-protein complexes?. Trends Cell Biol. 2004;14:226-32 pubmed
    ..We discuss how this surveillance capacity of the SMN complex might ensure assembly of Sm cores only on the correct RNAs and prevent illicit, potentially deleterious assembly of Sm cores on random RNAs. ..
  9. Boulon S, Verheggen C, Jady B, Girard C, Pescia C, Paul C, et al. PHAX and CRM1 are required sequentially to transport U3 snoRNA to nucleoli. Mol Cell. 2004;16:777-87 pubmed
    ..Surprisingly, we found that PHAX, the snRNA export adaptor, is highly enriched in complexes containing m7G-capped U3 precursors...

More Information

Publications15

  1. Watkins N, Lemm I, Ingelfinger D, Schneider C, Hossbach M, Urlaub H, et al. Assembly and maturation of the U3 snoRNP in the nucleoplasm in a large dynamic multiprotein complex. Mol Cell. 2004;16:789-98 pubmed
    ..assembly (TIP48, TIP49, Nopp140), RNA processing (TGS1, La, LSm4, hRrp46), and subcellular localization (CRM1, PHAX). Using RNAi, we show that most of these factors are essential for box C/D snoRNA accumulation...
  2. Mourão A, Varrot A, Mackereth C, Cusack S, Sattler M. Structure and RNA recognition by the snRNA and snoRNA transport factor PHAX. RNA. 2010;16:1205-16 pubmed publisher
    ..The phosphorylated adaptor for nuclear export (PHAX) is required for nuclear export of snRNAs in metazoans and also involved in the intranuclear transport of snoRNAs ..
  3. Gebhardt A, Habjan M, Benda C, Meiler A, Haas D, Hein M, et al. mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3. Nat Commun. 2015;6:8192 pubmed publisher
    ..However, NCBP3 becomes pivotal under stress conditions, such as virus infection. We propose the existence of an alternative CBC involving NCBP1 and NCBP3 that plays a key role in mRNA biogenesis. ..
  4. Schulze W, Cusack S. Structural basis for mutually exclusive co-transcriptional nuclear cap-binding complexes with either NELF-E or ARS2. Nat Commun. 2017;8:1302 pubmed publisher
    ..the direct interactions between CBC and NELF-E, a subunit of the negative elongation factor complex, ARS2 and PHAX. Our biochemical and crystal structure results show that the homologous C-terminal peptides of NELF-E and ARS2 bind ..
  5. Lemm I, Girard C, Kuhn A, Watkins N, Schneider M, Bordonné R, et al. Ongoing U snRNP biogenesis is required for the integrity of Cajal bodies. Mol Biol Cell. 2006;17:3221-31 pubmed
    ..coilin, U snRNPs, and proteins present in C/D box small nucleolar (sno)RNPs in cells depleted of hTGS1, SMN, or PHAX. Knockdown of any of these three proteins by RNAi interferes with U snRNP maturation before the reentry of U snRNA ..
  6. Shainyan B, Kirpichenko S, Kleinpeter E. Conformational Preferences of the Phenyl Group in 1-Phenyl-1-X-1-silacyclo-hexanes (X = MeO, HO) and 3-Phenyl-3-X-3-silatetrahydropyrans (X = HO, H) by Low Temperature 13C-NMR Spectroscopy and Theoretical Calculations. J Org Chem. 2017;: pubmed publisher
    ..The Phax ? Pheq equilibrium constants at 103 K are 2.21 for 1 and 4.59 for 4...