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Genomes and Genes | PDK3SummaryGene Symbol: PDK3 Description: pyruvate dehydrogenase kinase, isozyme 3 Alias: pyruvate dehydrogenase kinase, isoenzyme 3, pyruvate dehydrogenase, lipoamide, kinase isozyme 3, mitochondrial Species: human Top Publications
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Publications
Induction of pyruvate dehydrogenase kinase-3 by hypoxia-inducible factor-1 promotes metabolic switch and drug resistanceChun Wun Lu
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 1 University Road, Tainan 701, Taiwan
J Biol Chem 283:28106-14. 2008..Herein, we reported that hypoxia-inducible factor-1 (HIF-1) induced pyruvate dehydrogenase kinase-3 (PDK3) expression leading to inhibition of mitochondrial respiration...
Diversity of the pyruvate dehydrogenase kinase gene family in humansR Gudi
Department of Biochemistry and Molecular Biology, Indiana University, School of Medicine, Indianapolis 46202 5122, USA
J Biol Chem 270:28989-94. 1995..primary structures, two isoenzymes identified in humans correspond to rat PDK1 and PDK2, whereas a third gene (PDK3) encodes for a new isoenzyme that shares 68% and 67% of amino acid identities with PDK1 and PDK2, respectively...- Regulation of PDK mRNA by high fatty acid and glucose in pancreatic isletsJianxiang Xu
Kosair Children s Hospital Research Institute, Department of Pediatrics, University of Louisville School of Medicine, Louisville, KY 40292, USA
Biochem Biophys Res Commun 344:827-33. 2006..The gene and protein for PDK3 were not expressed in islets. Palmitate up-regulated mRNA expression of PDK1 (2.9-fold), PDK2 (1... - Structural determinants for cross-talk between pyruvate dehydrogenase kinase 3 and lipoyl domain 2 of the human pyruvate dehydrogenase complexShih Chia Tso
Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
J Biol Chem 281:27197-204. 2006..We showed previously that binding of the lipoyl domain 2 (L2) of the pyruvate dehydrogenase complex to PDK3 induces a "cross-tail" conformation in PDK3, resulting in an opening of the active site cleft and the .. - Pyruvate dehydrogenase kinase regulatory mechanisms and inhibition in treating diabetes, heart ischemia, and cancerT E Roche
Department of Biochemistry, Kansas State University, Chalmers Hall, Manhattan, KS 66506, USA
Cell Mol Life Sci 64:830-49. 2007..complex (PDC) in the active form is reduced by the activities of dedicated PD kinase isozymes (PDK1, PDK2, PDK3 and PDK4)... - Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17Ki Young Choi
Department of Biology, Yonsei University, Seoul 120 749, Republic of Korea
Biochem Biophys Res Commun 357:766-71. 2007..of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17... - Crystal structure of an asymmetric complex of pyruvate dehydrogenase kinase 3 with lipoyl domain 2 and its biological implicationsYancho Devedjiev
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, School of Medicine and Dentistry, Kaul Genetics Building, Birmingham, AL 35294, USA
J Mol Biol 370:407-16. 2007..Thus, the L2-domains likely act not only as the structural anchors but also modulate the catalytic cycle of PDHK... - Three members of the human pyruvate dehydrogenase kinase gene family are direct targets of the peroxisome proliferator-activated receptor beta/deltaTatjana Degenhardt
Department of Biochemistry, University of Kuopio, FIN 70211, Kuopio, Finland
J Mol Biol 372:341-55. 2007..In human embryonal kidney cells we found the mRNA expression of PDK2, PDK3 and PDK4 to be under direct primary control of PPAR ligands, and in normal mouse kidney tissue Pdk2 and Pdk4 are .. - Distinct structural mechanisms for inhibition of pyruvate dehydrogenase kinase isoforms by AZD7545, dichloroacetate, and radicicolMasato Kato
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Structure 15:992-1004. 2007..We have determined structures of human PDK1 or PDK3 bound to the inhibitors AZD7545, dichloroacetate (DCA), and radicicol... 
PDH activation during in vitro muscle contractions in PDH kinase 2 knockout mice: effect of PDH kinase 1 compensationEmily C Dunford
Centre for Muscle Metabolism and Biophysics, Brock University, St Catharines, Ontario, Canada
Am J Physiol Regul Integr Comp Physiol 300:R1487-93. 2011..PDH is deactivated by a set of PDH kinases (PDK1, PDK2, PDK3, PDK4), with PDK2 and PDK4 being the most predominant isoforms in skeletal muscle...- Overexpression of pyruvate dehydrogenase kinase 3 increases drug resistance and early recurrence in colon cancerChun Wun Lu
Department of Physiology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
Am J Pathol 179:1405-14. 2011..Pyruvate dehydrogenase kinase-1 (PDK1) and PDK3 participate in the metabolic switch of cancer cells; however, the medical significance of PDK1 and PDK3 in cancer .. - The carboxy-terminal tail of pyruvate dehydrogenase kinase 2 is required for the kinase activityAlla Klyuyeva
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Alabama, Birmingham, Alabama 35294, USA
Biochemistry 44:13573-82. 2005..We also show that the carboxyl tails derived from isozymes PDK1, PDK3, and PDK4 are capable of supporting the kinase activity of the kinase core derived from PDK2 as well as binding of .. - Crystal structure of pyruvate dehydrogenase kinase 3 bound to lipoyl domain 2 of human pyruvate dehydrogenase complexMasato Kato
Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
EMBO J 24:1763-74. 2005..b>PDK3 binds to the inner lipoyl domains (L2) from the 60-meric transacetylase (E2p) core of PDC, with concomitant .. - Evidence for existence of tissue-specific regulation of the mammalian pyruvate dehydrogenase complexM M Bowker-Kinley
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202 5122, USA
Biochem J 329:191-6. 1998Tissue distribution and kinetic parameters for the four isoenzymes of pyruvate dehydrogenase kinase (PDK1, PDK2, PDK3 and PDK4) identified thus far in mammals were analysed... - Marked differences between two isoforms of human pyruvate dehydrogenase kinaseJ C Baker
Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA
J Biol Chem 275:15773-81. 2000..the predominant changes in the rates of phosphorylation of the pyruvate dehydrogenase (E1) component by PDK2 and PDK3. E2 assembles as a 60-mer via its C-terminal domain and has mobile connections to an E1-binding domain and then two .. - Regulation of pyruvate dehydrogenase activity through phosphorylation at multiple sitesE Kolobova
Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110 2499, USA
Biochem J 358:69-77. 2001..of protein kinase responsible for the phosphorylation and inactivation of pyruvate dehydrogenase (PDK1, PDK2, PDK3 and PDK4) differ in their abilities to phosphorylate the enzyme... - Site specificity of four pyruvate dehydrogenase kinase isoenzymes toward the three phosphorylation sites of human pyruvate dehydrogenaseL G Korotchkina
Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York, Buffalo, New York 14214, USA
J Biol Chem 276:37223-9. 2001..site 1 and site 2, however, with different rates in phosphate buffer (for site 1, PDK2 > PDK4 approximately PDK1 > PDK3; for site 2, PDK3 > PDK4 > PDK2 > PDK1). Site 3 was phosphorylated by PDK1 only... - Selective modification of pyruvate dehydrogenase kinase isoform expression in rat pancreatic islets elicited by starvation and activation of peroxisome proliferator-activated receptor-alpha: implications for glucose-stimulated insulin secretionM C Sugden
Department of Diabetes and Metabolic Medicine, Division of General and Developmental Medicine, St Bartholomew s and the Royal London School of Medicine and Dentistry, Queen Mary, University of London, London, U K
Diabetes 50:2729-36. 2001..study, using antibodies against PDK1, PDK2, and PDK4 (no sufficiently specific antibodies are as yet available for PDK3), we identified the PDK isoform profile of the pancreatic islet and delineated the effects of starvation (48 h) on .. - Interaction between the individual isoenzymes of pyruvate dehydrogenase kinase and the inner lipoyl-bearing domain of transacetylase component of pyruvate dehydrogenase complexAlina Tuganova
Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110 2499, U S A
Biochem J 366:129-36. 2002..It was found that PDK isoenzymes are markedly different with respect to their affinities for L2. PDK3 demonstrated a very tight binding, which persisted during isolation of PDK3-L2 complexes using size-exclusion .. - Therapeutic potential of the mammalian pyruvate dehydrogenase kinases in the prevention of hyperglycaemiaM C Sugden
Department of Diabetes and Metabolic Medicine, Division of General and Developmental Medicine, St Bartholomew s and Royal London School of Medicine and Dentistry, Queen Mary, University of London, London E1 4NS, U K
Curr Drug Targets Immune Endocr Metabol Disord 2:151-65. 2002..in most major tissues and organs of the body, PDK1 appears to be limited to the heart and pancreatic islets, and PDK3 is limited to the kidney, brain and testis... - Formation of functional heterodimers by isozymes 1 and 2 of pyruvate dehydrogenase kinaseIgor Boulatnikov
Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri Kansas City, 5110 Rockhill Road, Kansas City, MO 64110 2499, USA
Biochim Biophys Acta 1645:183-92. 2003..genetically and biochemically distinct forms of PDK that are expressed in a tissue-specific manner (PDK1, PDK2, PDK3, and PDK4)... - Recent advances in mechanisms regulating glucose oxidation at the level of the pyruvate dehydrogenase complex by PDKsMary C Sugden
Department of Diabetes and Metabolic Medicine, Division of General and Developmental Medicine, Bart s and The London, Queen Mary s School of Medicine and Dentistry, University of London, Mile End Road, London E1 4NS, United Kingdom
Am J Physiol Endocrinol Metab 284:E855-62. 2003.... - Pyruvate dehydrogenase activation and kinase expression in human skeletal muscle during fastingLawrence L Spriet
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1
J Appl Physiol 96:2082-7. 2004..The changes in PDK-4 expression and PDH activity did not coincide with increases in the transcriptional activators PPAR-alpha and FKHR... - A novel Xp22.11 deletion causing a syndrome of craniosynostosis and periventricular nodular heterotopiaMargriet van Kogelenberg
Department of Paediatrics and Child Health, Dunedin School of Medicine, Otago University, Dunedin, New Zealand
Am J Med Genet A 155:3144-7. 2011..11 affecting all or part of three annotated genes, ZFX, PDK3, and PCYT1B in this subject...
Research Grants
- Structure and Function of Mitochondrial Protein KinasesDavid T Chuang; Fiscal Year: 2010..I.'s laboratory has solved the crystal structures for three (PDK1, PDK3 and PDK4) of the four PDK isoforms and various PDK-inhibitor/activator complexes. Based on these advances, the P.I...
- Structure and Function of Mitochondrial Protein KinasesDavid T Chuang; Fiscal Year: 2010..I.'s laboratory has solved the crystal structures for three (PDK1, PDK3 and PDK4) of the four PDK isoforms and various PDK-inhibitor/activator complexes. Based on these advances, the P.I...
- Structure and Function of Mitochondrial Protein KinasesDAVID CHUANG; Fiscal Year: 2009..I.'s laboratory has solved the crystal structures for three (PDK1, PDK3 and PDK4) of the four PDK isoforms and various PDK-inhibitor/activator complexes. Based on these advances, the P.I...
- REGULATION OF MAMMALIAN PYRUVATE DEHYDROGENASETHOMAS ROCHE; Fiscal Year: 2004..The long term objective is to understand the tissue-specific control of PDC and to delineate the unique properties of kinase isoforms overexpressed in diabetes which inactivates PDC thereby minimize glucose use. ..
- REGULATION OF MAMMALIAN PYRUVATE DEHYDROGENASETHOMAS ROCHE; Fiscal Year: 2000..Aberrant PDC operation is associated with diabetes and obesity. Inborn errors in components cause severe defects. Primary biliary cirrhosis is an autoimmune disease with antibodies to E2 and other PDC subunits. ..
- REGULATION OF MAMMALIAN PYRUVATE DEHYDROGENASETHOMAS ROCHE; Fiscal Year: 1990....