PDHB

Summary

Gene Symbol: PDHB
Description: pyruvate dehydrogenase (lipoamide) beta
Alias: PDHBD, PDHE1-B, PHE1B, pyruvate dehydrogenase E1 component subunit beta, mitochondrial, pyruvate dehydrogenase, E1 beta polypeptide
Species: human

Top Publications

  1. pmc The E1beta and E2 subunits of the Bacillus subtilis pyruvate dehydrogenase complex are involved in regulation of sporulation
    Haichun Gao
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Bacteriol 184:2780-8. 2002
  2. doi PDH E1β deficiency with novel mutations in two patients with Leigh syndrome
    E Quintana
    Institut de Bioquimica Clinica, Servei de Bioquímica i Genètica Molecular, Hospital Clinic, Barcelona, Spain
    J Inherit Metab Dis 32:S339-43. 2009
  3. pmc Transposon mutagenesis identified chromosomal and plasmid genes essential for adaptation of the marine bacterium Dinoroseobacter shibae to anaerobic conditions
    Matthias Ebert
    Institute for Microbiology, Technische Universitat Braunschweig, Braunschweig, Germany
    J Bacteriol 195:4769-77. 2013
  4. ncbi Toward a Wolbachia multilocus sequence typing system: discrimination of Wolbachia strains present in Drosophila species
    Charalampos Paraskevopoulos
    Department of Environmental and Natural Resources Management, University of Ioannina, 2 Seferi St, 30100, Agrinio, Greece
    Curr Microbiol 53:388-95. 2006
  5. ncbi Gene and primary structures of dye-linked L-proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
    Ryushi Kawakami
    Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima, 2 1 Minamijosanjimacho, Tokushima 770 8506, Japan
    Extremophiles 8:99-108. 2004
  6. pmc Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops
    Masato Kato
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 9038, USA
    Structure 16:1849-59. 2008
  7. ncbi Activity of pyruvate dehydrogenase A (PDHA) in hamster spermatozoa correlates positively with hyperactivation and is associated with sperm capacitation
    Vivek Kumar
    Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
    Biol Reprod 75:767-77. 2006
  8. doi Spectrum of neurological and survival outcomes in pyruvate dehydrogenase complex (PDC) deficiency: lack of correlation with genotype
    Suzanne D Debrosse
    Department of Genetics, 11100 Euclid Avenue, Cleveland, OH 44106, USA
    Mol Genet Metab 107:394-402. 2012
  9. doi RNA sequencing to study gene expression and single nucleotide polymorphism variation associated with citrate content in cow milk
    A Canovas
    Department of Animal Science, University of California Davis, One Shields Ave, Davis 95616, USA
    J Dairy Sci 96:2637-48. 2013
  10. doi Construction and validation of a GFP-based vector for promoter expression analysis in the fish pathogen Flavobacterium psychrophilum
    Esther Gomez
    Departamento de Biologia Funcional, Universidad de Oviedo, Oviedo, Asturias, Spain
    Gene 497:263-8. 2012

Scientific Experts

  • C Prasad
  • Markus Wallwiener
  • Ludwig Czibere
  • Jun Xia Cao
  • Winkins Santosh
  • Y Sasaki
  • S E Coupland
  • T Yamada
  • Anne Soisig Steunou
  • Michel Gohar
  • Ewa M Ciszak
  • M C Sugden
  • A Canovas
  • Cindy Thomas
  • Matthias Ebert
  • Esther Gomez
  • Suzanne D Debrosse
  • Aditya Venugopal
  • Chun Kai Yang
  • A Imbard
  • Carmen Wolf
  • N V L Serão
  • Imranul Alam
  • Zhen Cai
  • Colette M Maurer
  • Monica Busuioc
  • Shiek Ssj Ahmed
  • E Quintana
  • Felicity Johnson
  • K Okajima
  • Lioubov G Korotchkina
  • Masato Kato
  • Stephanie Hirner
  • Zongchao Han
  • Sanghyeob Lee
  • Vivek Kumar
  • Charalampos Paraskevopoulos
  • Yasuaki Hiromasa
  • Ruth M Brown
  • Ryushi Kawakami
  • Jake N Matic
  • Haichun Gao
  • Petra Tielen
  • J F Medrano
  • G Rincon
  • Roger Dumke
  • A Laubscher
  • Christoph Wittmann
  • A Islas-Trejo
  • Sebastian Laaß
  • Melanie Burghartz
  • Lars Wöhlbrand
  • Ralf Rabus
  • Dieter Jahn
  • Enno Jacobs
  • Jörn Petersen
  • Sebastian Koßmehl
  • R Jimenez-Flores
  • Lucía Fernández
  • D C Pauling
  • David Pérez-Pascual
  • José A Guijarro
  • Ghunwa Nakouzi
  • Kazuki Okajima
  • Roberto Navais
  • Shulin Zhang
  • Douglas S Kerr
  • M Van Poucke
  • Jessica Méndez
  • George Grahame
  • Mary Beth Frohnapfel
  • M Caruso
  • Marilyn Lusk-Kopp
  • Pilar Reimundo
  • Christine L Schmotzer
  • Kyu Rhee
  • Ahmed T Abdelal
  • Pascal Rainard
  • Dirk Schnappinger
  • Phang C Tai
  • P de Lonlay
  • C Marsac
  • Hosam E Ewis
  • M Mine
  • L L Verardo
  • C Vequaud
  • Wolfram Petzl
  • J Braccini Neto
  • J M Saudubray
  • Dirk Albrecht

Detail Information

Publications60

  1. pmc The E1beta and E2 subunits of the Bacillus subtilis pyruvate dehydrogenase complex are involved in regulation of sporulation
    Haichun Gao
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Bacteriol 184:2780-8. 2002
    ..Disruptions of the pdhB, pdhC, and pdhD genes were isolated, but attempts to construct a pdhA mutant were unsuccessful, suggesting that ..
  2. doi PDH E1β deficiency with novel mutations in two patients with Leigh syndrome
    E Quintana
    Institut de Bioquimica Clinica, Servei de Bioquímica i Genètica Molecular, Hospital Clinic, Barcelona, Spain
    J Inherit Metab Dis 32:S339-43. 2009
    ..Only seven patients with deficiency of the E(1)β subunit have been described, with mutations in the PDHB gene in six of them. Clinically they presented with a non-specific encephalomyopathy...
  3. pmc Transposon mutagenesis identified chromosomal and plasmid genes essential for adaptation of the marine bacterium Dinoroseobacter shibae to anaerobic conditions
    Matthias Ebert
    Institute for Microbiology, Technische Universitat Braunschweig, Braunschweig, Germany
    J Bacteriol 195:4769-77. 2013
    ..Additional essential components (ferredoxin, cccA) of the anaerobic electron transfer chain and central metabolism (pdhB) were identified...
  4. ncbi Toward a Wolbachia multilocus sequence typing system: discrimination of Wolbachia strains present in Drosophila species
    Charalampos Paraskevopoulos
    Department of Environmental and Natural Resources Management, University of Ioannina, 2 Seferi St, 30100, Agrinio, Greece
    Curr Microbiol 53:388-95. 2006
    ..We report a new phylogenetic characterization of four genes (aspC, atpD, sucB, and pdhB), and provide an expanded analysis of markers described in previous studies (16S rDNA, ftsZ, groEL, dnaA, and gltA)...
  5. ncbi Gene and primary structures of dye-linked L-proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex
    Ryushi Kawakami
    Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima, 2 1 Minamijosanjimacho, Tokushima 770 8506, Japan
    Extremophiles 8:99-108. 2004
    ..The gene formed an operon that consisted of four genes, pdhA, pdhB, pdhF, and pdhX, which are tandemly arranged in the order of pdhA-F-X-B...
  6. pmc Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops
    Masato Kato
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 9038, USA
    Structure 16:1849-59. 2008
    ..This results in the disruption of the substrate channeling in the PDC, leading to the inactivation of this catalytic machine...
  7. ncbi Activity of pyruvate dehydrogenase A (PDHA) in hamster spermatozoa correlates positively with hyperactivation and is associated with sperm capacitation
    Vivek Kumar
    Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
    Biol Reprod 75:767-77. 2006
    ..using antibodies to PDHA, which is the active tetrameric protein that consists of a homodimer of PDHA2 and PDHB. Both immunofluorescence and confocal studies indicated a unique non-canonical, extramitochondrial localization for ..
  8. doi Spectrum of neurological and survival outcomes in pyruvate dehydrogenase complex (PDC) deficiency: lack of correlation with genotype
    Suzanne D Debrosse
    Department of Genetics, 11100 Euclid Avenue, Cleveland, OH 44106, USA
    Mol Genet Metab 107:394-402. 2012
    ..32 F), who were confirmed to have PDC deficiency with defined mutations in one of the genes of PDC (PDHA1, n = 53; PDHB, n = 4; DLAT, n = 2), including 47 different mutations, of which 22 were novel, and for whom clinical records and/..
  9. doi RNA sequencing to study gene expression and single nucleotide polymorphism variation associated with citrate content in cow milk
    A Canovas
    Department of Animal Science, University of California Davis, One Shields Ave, Davis 95616, USA
    J Dairy Sci 96:2637-48. 2013
    ..metabolic pathway genes [isocitrate dehydrogenase 1 (NADP+), soluble (IDH1); pyruvate dehydrogenase (lipoamide) β (PDHB); pyruvate kinase (PKM2); and solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1 (..
  10. doi Construction and validation of a GFP-based vector for promoter expression analysis in the fish pathogen Flavobacterium psychrophilum
    Esther Gomez
    Departamento de Biologia Funcional, Universidad de Oviedo, Oviedo, Asturias, Spain
    Gene 497:263-8. 2012
    ..psychrophilum, corresponding to genes fpp2-fpp1, pdhB and gldJ, under different growth conditions. Overall, the construction of pCP23-G facilitates genetic analysis in F...
  11. doi Intraabdominal adhesion formation is associated with differential mRNA expression of metabolic genes PDHb and SDHa
    Markus Wallwiener
    Department of Obstetrics and Gynaecology, Heidelberg University Hospital, Vossstr 9, 69115, Heidelberg, Germany
    Arch Gynecol Obstet 286:683-6. 2012
    ..We therefore investigated the gene expression of HIF-1 and two pivotal metabolic genes, pyruvate-dehydrogenaseβ (PDHb) and succinate-dehydrogenase-complex-subunit-A (SDHa) in a validated ischemia model of adhesion formation.
  12. doi Characterization of pyruvate dehydrogenase subunit B and enolase as plasminogen-binding proteins in Mycoplasma pneumoniae
    Cindy Thomas
    Dresden University of Technology, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D 01307 Dresden, Germany
    Microbiology 159:352-65. 2013
    ..Here, we demonstrated that the glycolytic enzymes enolase (Mpn606) and pyruvate dehydrogenase subunit B (Mpn392; PDHB) of M. pneumoniae show concentration-dependent binding to human plasminogen...
  13. pmc Genes influencing spinal bone mineral density in inbred F344, LEW, COP, and DA rats
    Imranul Alam
    Department of Biomedical Engineering, Indiana University Purdue University Indianapolis, 46202 5251, USA
    Funct Integr Genomics 10:63-72. 2010
    ..90) with lumbar vBMD. Of these, 14 genes (Akap1, Asgr2, Esd, Fam101b, Irf1, Lcp1, Ltc4s, Mdp-1, Pdhb, Plxdc1, Rabep1, Rhot1, Slc2a4, Xpo4) were confirmed by qPCR...
  14. pmc Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders
    Felicity Johnson
    Department of Neurological Surgery, Cornell University, Weill Medical College, New York, New York, USA
    PLoS ONE 4:e7100. 2009
    ..One candidate, the chaperone HSPA8, was common to each independent study. Two others (PDHB subunit and IDH3A subunit) did not appear to bind to OMI, however persisted in the mito-proteome when OMI was ..
  15. doi Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss
    Sarah E Coupland
    Department of Pathology, School of Cancer Studies, University of Liverpool, Liverpool, United Kingdom
    Invest Ophthalmol Vis Sci 51:12-20. 2010
    ..To compare the proteomic profiles of primary uveal melanomas, with and without loss of chromosome 3...
  16. doi Mutations of the E1beta subunit gene (PDHB) in four families with pyruvate dehydrogenase deficiency
    K Okajima
    Center for Inherited Disorders of Energy Metabolism, Rainbow Babies and Childrens Hospital, University Hospitals Case Medical Center, Department of Pediatrics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 6004, USA
    Mol Genet Metab 93:371-80. 2008
    ..PDHA1), with fewer cases resulting from mutations in genes for E3, E3-binding protein, E2, and the E1beta subunit (PDHB). We have found four cases of PDHB mutations among 83 analyzed cases of PDC deficiency...
  17. pmc Metabolic profiling of Parkinson's disease: evidence of biomarker from gene expression analysis and rapid neural network detection
    Shiek Ssj Ahmed
    Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, 603 203, India
    J Biomed Sci 16:63. 2009
    ..To test this hypothesis, we used proton NMR and multivariate analysis followed by neural network pattern detection...
  18. doi MuRF1-dependent regulation of systemic carbohydrate metabolism as revealed from transgenic mouse studies
    Stephanie Hirner
    Institute for Anaesthesiology and Intensive Operative Care, Medical Faculty Mannheim, Mannheim 68167, Germany
    J Mol Biol 379:666-77. 2008
    ....
  19. pmc Binding of pyruvate dehydrogenase to the core of the human pyruvate dehydrogenase complex
    Lioubov G Korotchkina
    Department of Biochemistry, School of Medicine and Biomedical Sciences, University at Buffalo, The State University of New York, Buffalo, NY 14214, USA
    FEBS Lett 582:468-72. 2008
    ..This latter finding shows species specificity in the interaction between hE1beta and hE2 in PDC...
  20. doi Distinct retinal deficits in a zebrafish pyruvate dehydrogenase-deficient mutant
    Colette M Maurer
    University of Zurich, Institute of Molecular Life Sciences, Neuroscience Center Zurich and Center for Integrative Human Physiology, Zurich, Switzerland
    J Neurosci 30:11962-72. 2010
    ..One such example is the zebrafish noir mutant, which we found to be mutated in the pdhb gene, coding for the E1 beta subunit of the pyruvate dehydrogenase complex...
  21. pmc Pluripotency-associated genes in human nasopharyngeal carcinoma CNE-2 cells are reactivated by a unique epigenetic sub-microenvironment
    Jun Xia Cao
    State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat Sen University, 650 Dongfeng Road, Guangzhou 510060, PR China
    BMC Cancer 10:68. 2010
    ..In this study we found that the phenotype of SP can be "generated" by macrophage-like cells under conditioned culture. Furthermore the gene regulation pathway involved in cellular reprogramming process was investigated...
  22. doi Molecular characterization of 82 patients with pyruvate dehydrogenase complex deficiency. Structural implications of novel amino acid substitutions in E1 protein
    A Imbard
    Biochimie Hôpital de Bicêtre, Hôpitaux Universitaires Paris Sud, AP HP, Paris, France
    Mol Genet Metab 104:507-16. 2011
    ..cases result from mutations in the X-linked gene for the pyruvate dehydrogenase E1α subunit (PDHA1) while a few cases result from mutations in genes for E1β (PDHB), E2 (DLAT), E3 (DLD) and E3BP (PDHX) subunits or PDH-phosphatase (PDP1).
  23. doi Pyruvate dehydrogenase deficiency and epilepsy
    Chitra Prasad
    Department of Pediatrics, University of Western Ontario, London, Ontario, Canada
    Brain Dev 33:856-65. 2011
    ..E1-alpha polypeptide (PDHA1) deficiency and the second one with pyruvate dehydrogenase complex E1-beta subunit (PDHB) deficiency (a rare subtype of PDHc deficiency)) and a selected review of published case series...
  24. pmc Profiling trait anxiety: transcriptome analysis reveals cathepsin B (Ctsb) as a novel candidate gene for emotionality in mice
    Ludwig Czibere
    Max Planck Institute of Psychiatry, Munich, Germany
    PLoS ONE 6:e23604. 2011
    ..5 (Enpp5), high mobility group nucleosomal binding domain 3 (Hmgn3) and pyruvate dehydrogenase beta (Pdhb). Additionally, we confirmed brain region-specific differences in the expression of synaptotagmin 4 (Syt4)...
  25. pmc Nonclassical protein secretion by Bacillus subtilis in the stationary phase is not due to cell lysis
    Chun Kai Yang
    Department of Biology, Georgia State University, Atlanta, Georgia 30303, USA
    J Bacteriol 193:5607-15. 2011
    ..which all have defined cytoplasmic functions, include GroEL, DnaK, enolase, pyruvate dehydrogenase subunits PdhB and PdhD, and SodA...
  26. doi Genomic and proteomic characterization of Staphylococcus aureus mastitis isolates of bovine origin
    Carmen Wolf
    Institute for Microbiology, University of Greifswald, Greifswald, Germany
    Proteomics 11:2491-502. 2011
    ..Merely 12 proteins were expressed in at least 80% of the isolates, i.e. Atl, Aur, GlpQ, Hla, LtaS, Nuc, PdhB, SAB0846, SAB2176, SAB0566, SspA, and SspB forming the core exoproteome...
  27. ncbi Polymorphisms in promoter regions of PDHB, SORBS1, and EDG1 genes showing marbling-associated expression changes
    Takahisa Yamada
    Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Sakyoku, Kyoto 606 8502, Japan
    Res Commun Mol Pathol Pharmacol 120:105-14. 2007
    We have recently showed that the pyruvate dehydrogenase (lipoamide) beta (PDHB) gene involved in fatty acid oxidation, the sorbin and SH3 domain containing 1 (SORBS1) gene involved in insulin-stimulated glucose uptake, and the endothelial ..
  28. pmc Virulence of Mycobacterium tuberculosis depends on lipoamide dehydrogenase, a member of three multienzyme complexes
    Aditya Venugopal
    Department of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY 10065, USA
    Cell Host Microbe 9:21-31. 2011
    ..DlaT is absent, Mtb upregulates an Lpd-dependent branched-chain keto acid dehydrogenase (BCKADH) encoded by pdhA, pdhB, pdhC, and lpdC...
  29. doi Candidate gene expression and intramuscular fat content in pigs
    N V L Serão
    Departamento de Zootecnia, Universidade Federal de Vicosa, Vicosa, Brazil
    J Anim Breed Genet 128:28-34. 2011
    ..animal was extracted and correlated with candidate gene mRNA expression (ATN1, EEF1A2, FABP3, LDLR, MGP, OBSCN, PDHB, TRDN and RYR1). Within slaughter weight of 120 kg, Piau and Crossbred pigs showed higher IMF content (p < 0...
  30. pmc A combined proteomics and metabolomics profiling of gastric cardia cancer reveals characteristic dysregulations in glucose metabolism
    Zhen Cai
    Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China
    Mol Cell Proteomics 9:2617-28. 2010
    ..enzymes, two subunits controlling pyruvic acid efflux, lactate dehydrogenase A (LDHA) and pyruvate dehydrogenase B (PDHB), were further analyzed in vitro...
  31. pmc The pdh operon is expressed in a subpopulation of stationary-phase bacteria and is important for survival of sugar-starved Streptococcus mutans
    Monica Busuioc
    Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA
    J Bacteriol 192:4395-402. 2010
    ..We show that pdhD and the downstream genes pdhA, pdhB, and pdhC form an operon that is predominantly transcribed in stationary phase...
  32. ncbi Pyruvate dehydrogenase complex deficiency caused by ubiquitination and proteasome-mediated degradation of the E1 subunit
    Zongchao Han
    Department of Pediatrics, Division of Cellular and Molecular Therapy, Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA
    J Biol Chem 283:237-43. 2008
    ..Here we describe a case of PDH deficiency associated with the PDH E1beta subunit (PDHB) gene. The clinical phenotype of the patient was consistent with reported cases of PDH deficiency...
  33. pmc Transcriptional regulation of the respiratory genes in the cyanobacterium Synechocystis sp. PCC 6803 during the early response to glucose feeding
    Sanghyeob Lee
    Laboratory of Plant Genomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305 806, Korea
    Plant Physiol 145:1018-30. 2007
    ..Transcript levels of the four genes gpi, zwf, pdhB, and atpB started to increase along with a net increase in NAD(P)H, while the onset of net NAD(P)H consumption ..
  34. pmc Biochemical and molecular characterization of the Alcaligenes eutrophus pyruvate dehydrogenase complex and identification of a new type of dihydrolipoamide dehydrogenase
    S Hein
    Institut fur Mikrobiologie, Georg August Universität zu Göttingen, Germany
    J Bacteriol 176:4394-408. 1994
    ..eutrophus pyruvate dehydrogenase complex, pdhA (2,685 bp), pdhB (1,659 bp), and pdhL (1,782 bp), encoding the pyruvate dehydrogenase (E1), the dihydrolipoamide acetyltransferase (..
  35. ncbi The human myocardial two-dimensional gel protein database: update 1994
    J M Corbett
    Department of Cardiothoracic Surgery, National Heart and Lung Institute, Heart Science Centre, Middlesex, UK
    Electrophoresis 15:1459-65. 1994
    ..Electrophoresis) 1994, 15, 685-607). This database is being used in studies of alterations in protein expression in the diseased and transplanted human heart...
  36. ncbi Recombinant expression and evaluation of the lipoyl domains of the dihydrolipoyl acetyltransferase component of the human pyruvate dehydrogenase complex
    S Liu
    Department of Biochemistry, Kansas State University, Manhattan 66406
    Arch Biochem Biophys 316:926-40. 1995
    ..Thus, we have produced lipoyl domain constructs that can be employed in sorting the specific roles of E2L1 and E2L2 in facilitating catalytic and regulatory processes...
  37. pmc Cloning and sequencing of cDNAs encoding alpha and beta subunits of human pyruvate dehydrogenase
    K Koike
    Department of Pathological Biochemistry, Nagasaki University School of Medicine, Japan
    Proc Natl Acad Sci U S A 85:41-5. 1988
    ..8 kb for PDH alpha and 1.7 kb for PDH beta, respectively. The precursor proteins of PDH alpha and PDH beta were detected by immunoprecipitation from an 35S-labeled cell-free translation system...
  38. ncbi Identification of a cDNA clone for the beta-subunit of the pyruvate dehydrogenase component of human pyruvate dehydrogenase complex
    L Ho
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106
    Biochem Biophys Res Commun 150:904-8. 1988
    ..In Northern analysis of total RNA extracted from human heart, the E1 beta cDNA clone hybridized to a major 1.6 kb and a minor 5.2 kb RNA species...
  39. pmc Molecular cloning and characterization of human pyruvate dehydrogenase beta subunit gene
    K Koike
    Department of Pathological Biochemistry, Nagasaki University School of Medicine, Japan
    Proc Natl Acad Sci U S A 87:5594-7. 1990
    ..Primer-extension analysis indicated that the PDH beta gene transcription start site is an adenine residue located 132 bases upstream from the initiation codon in exon 1...
  40. ncbi Characterization of two cDNA clones for pyruvate dehydrogenase E1 beta subunit and its regulation in tricarboxylic acid cycle-deficient fibroblast
    T L Huh
    Laboratory of Metabolism and Molecular Biology, National Institute on Alcohol Abuse and Alcoholism, Rockville, Maryland 20852
    J Biol Chem 265:13320-6. 1990
    ....
  41. ncbi Cloning and cDNA sequence of the beta-subunit component of human pyruvate dehydrogenase complex
    L Ho
    Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106
    Gene 86:297-302. 1990
    ..The composite human liver E1 beta cDNA generated in this study provides a reference sequence for investigating the structure-function relationship of human E1 beta and for characterizing genetic mutations in patients with E1 deficiency...
  42. ncbi Isolation of tryptic fragment of antigen from mitochondrial inner membrane proteins reacting with antimitochondrial antibody in sera of patients with primary biliary cirrhosis
    D Muno
    Department of Biochemistry, Juntendo University, School of Medicine, Tokyo, Japan
    Hepatology 11:16-23. 1990
    ..Its amino acid composition is in good agreement with that of the subunit binding domain of the pyruvate dehydrogenase complex E2 from bovine heart...
  43. ncbi Cloning and sequence analysis of the genes encoding the alpha and beta subunits of the E1 component of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus
    C F Hawkins
    Department of Biochemistry, University of Cambridge, England
    Eur J Biochem 191:337-46. 1990
    ..Its nucleotide sequence was determined. Open reading frames (pdhA, pdhB) corresponding to the E1 alpha subunit (368 amino acids, Mr 41,312, without the initiating methionine residue) and ..
  44. pmc Three genes for enzymes of the pyruvate dehydrogenase complex map to human chromosomes 3, 7, and X
    S Olson
    National Institutes of Health 20892
    Am J Hum Genet 46:340-9. 1990
    ..Restrictive-fragment-length polymorphisms have been identified with E3, which will permit further localization of this gene by genetic linkage analysis...
  45. pmc Purification of the pyruvate dehydrogenase multienzyme complex of Zymomonas mobilis and identification and sequence analysis of the corresponding genes
    U Neveling
    Institut fur Biotechnologie, Forschungszentrum Julich, Germany
    J Bacteriol 180:1540-8. 1998
    ..The pdhB (1,323 bp) and lpd (1,401 bp) genes, encoding the E2 and E3 components, were identified in an unrelated gene region ..
  46. ncbi Identification of the catalytic glutamate in the E1 component of human pyruvate dehydrogenase
    R Fang
    Department of Molecular Biochemistry, Jilin University, Changchun, PR China
    FEBS Lett 437:273-7. 1998
    ..Human E1 was expressed in Escherichia coli and purified. Mutating Glu89 to glutamine, aspartate and alanine markedly reduces catalytic activity and the affinity for ThDP, consistent with a role as the catalytic glutamate...
  47. ncbi Organization of the cores of the mammalian pyruvate dehydrogenase complex formed by E2 and E2 plus the E3-binding protein and their capacities to bind the E1 and E3 components
    Yasuaki Hiromasa
    Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA
    J Biol Chem 279:6921-33. 2004
    ..Twelve E3 dimers were bound per E248.E3BP12 mass, which is consistent with this model...
  48. pmc In situ analysis of nitrogen fixation and metabolic switching in unicellular thermophilic cyanobacteria inhabiting hot spring microbial mats
    Anne Soisig Steunou
    Department of Plant Biology, The Carnegie Institution, 260 Panama Street, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 103:2398-403. 2006
    ..In contrast, transcripts encoding enzymes that may participate in fermentation fell into two categories; some (ldh, pdhB, ald, and ackA) decreased in the evening, whereas others (pflB, pflA, adhE, and acs) increased at the end of the ..
  49. ncbi Exploration of genes showing intramuscular fat deposition-associated expression changes in musculus longissimus muscle
    Y Sasaki
    Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Anim Genet 37:40-6. 2006
    ..Among the 35 annotated genes, 6 (BTG2, PDHB, SORBS1, TRDN, TTN and MGP) have been related to changes in intramuscular fat deposition, possibly by exerting ..
  50. ncbi A comparative study of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis extracellular proteomes
    Michel Gohar
    INRA, Génétique microbienne et Environnement, La Minière, Guyancourt, France
    Proteomics 5:3696-711. 2005
    ..Cytosolic proteins included enolase, GroEL, PdhB, PdhD, SodA and others. Cell surface proteins were mainly autolysins, proteases, nucleotidases and OppAs...
  51. ncbi Mutations in the gene for the E1beta subunit: a novel cause of pyruvate dehydrogenase deficiency
    Ruth M Brown
    Genetics Unit, Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU, Oxford, UK
    Hum Genet 115:123-7. 2004
    ..Activity of the PDH complex was restored in cultured fibroblasts from both patients by transfection and expression of the normal E1beta coding sequence...
  52. ncbi The pyruvate dehydrogenase complex of Mycoplasma hyopneumoniae contains a novel lipoyl domain arrangement
    Jake N Matic
    Microbiology and Immunology Section, Elizabeth Macarthur Agricultural Institute, Private Mail Bag 8, Camden, NSW, Australia
    Gene 319:99-106. 2003
    The genes encoding the pyruvate dehydrogenase (PDH) complex (pdhA, pdhB, pdhC and pdhD) from Mycoplasma hyopneumoniae have been cloned and sequenced...
  53. ncbi Recent advances in mechanisms regulating glucose oxidation at the level of the pyruvate dehydrogenase complex by PDKs
    Mary C Sugden
    Department of Diabetes and Metabolic Medicine, Division of General and Developmental Medicine, Bart s and The London, Queen Mary s School of Medicine and Dentistry, University of London, Mile End Road, London E1 4NS, United Kingdom
    Am J Physiol Endocrinol Metab 284:E855-62. 2003
    ....
  54. ncbi Structural basis for flip-flop action of thiamin pyrophosphate-dependent enzymes revealed by human pyruvate dehydrogenase
    Ewa M Ciszak
    Biological and Physical Space Research Laboratory, National Aeronautics and Space Administration Marshall Space Flight Center and Universities Space Research Association, Huntsville, Alabama 35812, USA
    J Biol Chem 278:21240-6. 2003
    ....
  55. ncbi Activation and mitochondrial translocation of protein kinase Cdelta are necessary for insulin stimulation of pyruvate dehydrogenase complex activity in muscle and liver cells
    M Caruso
    Dipartimento di Biologia e Patologia Cellulare e Molecolare and Centro di Endocrinologia ed Oncologia Sperimentale del CNR, Federico II University of Naples, 80131 Naples, Italy
    J Biol Chem 276:45088-97. 2001
    ..These events are necessary for insulin activation of the PDH complex in these cells...
  56. ncbi Integration of porcine chromosome 13 maps
    M Van Poucke
    Department of Animal Nutrition, Genetics, Breeding and Ethology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
    Cytogenet Cell Genet 93:297-303. 2001
    ..ACAA1, ACPP, B4GALT4, LTF, MYLK, PDHB and RARB...
  57. ncbi Azorhizobium caulinodans pyruvate dehydrogenase activity is dispensable for aerobic but required for microaerobic growth
    D C Pauling
    Department of MCD Biology, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064, USA
    Microbiology 147:2233-45. 2001
    Azorhizobium caulinodans mutant 62004 carries a null allele of pdhB, encoding the E1beta subunit of pyruvate dehydrogenase, which converts pyruvate to acetyl-CoA...
  58. ncbi Symbiotic induction of pyruvate dehydrogenase genes from Sinorhizobium meliloti
    D Cabanes
    Laboratoire de Biologie Moleculaire des relations Plantes Microorganismes, CNRS INRA, Castanet Tolosan, France
    Mol Plant Microbe Interact 13:483-93. 2000
    ..pdhAalpha and pdhAbeta precede pdhB (1,344 bp), which encodes the E2p component, dihydrolipoamide acetyltransferase, of the PDHc...
  59. ncbi Isolation, characterization and chromosomal localization of cDNA clones for the E1 beta subunit of the pyruvate dehydrogenase complex
    K Chun
    Department of Pediatrics, University of Toronto, Hospital for Sick Children, Canada
    Eur J Biochem 194:587-92. 1990
    ..A motif AXGXXXXGL(R/K)X15(D/E)Q was found in common with a variety of other oxo-acid oxidoreductases, but its function is not known...