PCNA

Summary

Gene Symbol: PCNA
Description: proliferating cell nuclear antigen
Alias: ATLD2, proliferating cell nuclear antigen, DNA polymerase delta auxiliary protein, cyclin
Species: human
Products:     PCNA

Top Publications

  1. De Biasio A, Sanchez R, Prieto J, Villate M, Campos Olivas R, Blanco F. Reduced stability and increased dynamics in the human proliferating cell nuclear antigen (PCNA) relative to the yeast homolog. PLoS ONE. 2011;6:e16600 pubmed publisher
    b>Proliferating Cell Nuclear Antigen (PCNA) is an essential factor for DNA replication and repair. PCNA forms a toroidal, ring shaped structure of 90 kDa by the symmetric association of three identical monomers...
  2. Huang T, Nijman S, Mirchandani K, Galardy P, Cohn M, Haas W, et al. Regulation of monoubiquitinated PCNA by DUB autocleavage. Nat Cell Biol. 2006;8:339-47 pubmed
    ..we show that the DUB ubiquitin specific protease 1 (USP1) deubiquitinates the DNA replication processivity factor, PCNA, as a safeguard against error-prone translesion synthesis (TLS) of DNA...
  3. Bruning J, Shamoo Y. Structural and thermodynamic analysis of human PCNA with peptides derived from DNA polymerase-delta p66 subunit and flap endonuclease-1. Structure. 2004;12:2209-19 pubmed
    ..protein-protein interactions with DNA replication and repair proteins through a small peptide motif termed the PCNA-interacting protein, or PIP-box...
  4. Fan J, Otterlei M, Wong H, Tomkinson A, Wilson D. XRCC1 co-localizes and physically interacts with PCNA. Nucleic Acids Res. 2004;32:2193-201 pubmed
    ..We report here that XRCC1 co-localizes with proliferating cell nuclear antigen (PCNA) at DNA replication foci, observed exclusively in the S phase of undamaged HeLa cells...
  5. Ogi T, Limsirichaikul S, Overmeer R, Volker M, Takenaka K, Cloney R, et al. Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells. Mol Cell. 2010;37:714-27 pubmed publisher
    ..Pol kappa is recruited to repair sites by ubiquitinated PCNA and XRCC1 and pol delta by the classical replication factor complex RFC1-RFC, together with a polymerase accessory ..
  6. Moldovan G, Dejsuphong D, Petalcorin M, Hofmann K, Takeda S, Boulton S, et al. Inhibition of homologous recombination by the PCNA-interacting protein PARI. Mol Cell. 2012;45:75-86 pubmed publisher
    ..In yeast, the UvrD family helicase Srs2 is recruited to sites of DNA replication by SUMO-modified PCNA, where it acts to restrict HR by disassembling toxic RAD51 nucleofilaments...
  7. Bienko M, Green C, Sabbioneda S, Crosetto N, Matic I, Hibbert R, et al. Regulation of translesion synthesis DNA polymerase eta by monoubiquitination. Mol Cell. 2010;37:396-407 pubmed publisher
    ..Its action is initiated by simultaneous interaction between the PIP box in pol eta and PCNA and between the UBZ in pol eta and monoubiquitin attached to PCNA...
  8. Jiang H, Hickey R, Abdel Aziz W, Tom T, Wills P, Liu J, et al. Human cell DNA replication is mediated by a discrete multiprotein complex. J Cell Biochem. 2002;85:762-74 pubmed
    ..in vitro DNA synthetic activity, and by Western blot analysis, contains DNA polymerase delta, proliferating cell nuclear antigen, and replication protein A...
  9. Li H, Xie B, Zhou Y, Rahmeh A, Trusa S, Zhang S, et al. Functional roles of p12, the fourth subunit of human DNA polymerase delta. J Biol Chem. 2006;281:14748-55 pubmed
    ..as follows: the catalytic subunit; p125, which is tightly associated with the p50 subunit; p68, a proliferating cell nuclear antigen (PCNA)-binding protein; and a fourth subunit, p12...

More Information

Publications137 found, 100 shown here

  1. Lin J, Zeman M, Chen J, Yee M, Cimprich K. SHPRH and HLTF act in a damage-specific manner to coordinate different forms of postreplication repair and prevent mutagenesis. Mol Cell. 2011;42:237-49 pubmed publisher
    ..Following UV, HLTF enhances PCNA monoubiquitination and recruitment of TLS polymerase η, while also inhibiting SHPRH function...
  2. Vidal A, Kannouche P, Podust V, Yang W, Lehmann A, Woodgate R. Proliferating cell nuclear antigen-dependent coordination of the biological functions of human DNA polymerase iota. J Biol Chem. 2004;279:48360-8 pubmed
    ..We report here that an interaction between human DNA polymerase iota (poliota) and the proliferating cell nuclear antigen (PCNA) stimulates the processivity of poliota in a template-dependent manner in vitro...
  3. Takami Y, Ono T, Fukagawa T, Shibahara K, Nakayama T. Essential role of chromatin assembly factor-1-mediated rapid nucleosome assembly for DNA replication and cell division in vertebrate cells. Mol Biol Cell. 2007;18:129-41 pubmed
    ..showed that the binding abilities of CAF-1p150 with CAF-1p60 and DNA polymerase sliding clamp proliferating cell nuclear antigen (PCNA) but not with heterochromatin protein HP1-gamma are required for cell viability...
  4. Merkle C, Karnitz L, Henry Sánchez J, Chen J. Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment. J Biol Chem. 2003;278:30051-6 pubmed
    ..This alternative RFC-containing complex interacts with proliferating cell nuclear antigen but not with the Rad9/Rad1/Hus1 complex, a proliferating cell nuclear antigen-like clamp involved in ..
  5. Zhao H, Chen M, Lo Y, Waltz S, Wang J, Ho P, et al. The Ron receptor tyrosine kinase activates c-Abl to promote cell proliferation through tyrosine phosphorylation of PCNA in breast cancer. Oncogene. 2014;33:1429-37 pubmed publisher
    ..The sliding clamp proliferating cell nuclear antigen (PCNA) is an indispensable component of the DNA machinery responsible for replicating the genome and ..
  6. Plosky B, Vidal A, Fernández de Henestrosa A, McLenigan M, McDonald J, Mead S, et al. Controlling the subcellular localization of DNA polymerases iota and eta via interactions with ubiquitin. EMBO J. 2006;25:2847-55 pubmed
    ..that both polymerases interact noncovalently with free polyUb chains, as well as mono-ubiquitinated proliferating cell nuclear antigen (Ub-PCNA)...
  7. Hishiki A, Hashimoto H, Hanafusa T, Kamei K, Ohashi E, Shimizu T, et al. Structural basis for novel interactions between human translesion synthesis polymerases and proliferating cell nuclear antigen. J Biol Chem. 2009;284:10552-60 pubmed publisher
    ..These enzymes show preferential bypass for different lesions. Proliferating cell nuclear antigen (PCNA), which functions as a sliding clamp for the replicative polymerase Poldelta, also interacts ..
  8. Cohn M, Kowal P, Yang K, Haas W, Huang T, Gygi S, et al. A UAF1-containing multisubunit protein complex regulates the Fanconi anemia pathway. Mol Cell. 2007;28:786-97 pubmed
    ..Taken together, our results describe a mechanism of regulation of the deubiquitinating enzyme, USP1, and of DNA repair. ..
  9. Kirchmaier A. Ub-family modifications at the replication fork: Regulating PCNA-interacting components. FEBS Lett. 2011;585:2920-8 pubmed publisher
    ..to the replication fork in a dynamic and coordinated manner through physical interactions with Proliferating Cell Nuclear Antigen, PCNA...
  10. Lo Y, Ho P, Wang S. Epidermal growth factor receptor protects proliferating cell nuclear antigen from cullin 4A protein-mediated proteolysis. J Biol Chem. 2012;287:27148-57 pubmed publisher
    b>Proliferating cell nuclear antigen (PCNA) is an essential component for DNA synthesis upon growth stimulation...
  11. Yuan J, Ghosal G, Chen J. The HARP-like domain-containing protein AH2/ZRANB3 binds to PCNA and participates in cellular response to replication stress. Mol Cell. 2012;47:410-21 pubmed publisher
    ..Furthermore, AH2 binds to PCNA, which is crucial for its function at stalled replication forks...
  12. Baynton K, Otterlei M, Bjørås M, Von Kobbe C, Bohr V, Seeberg E. WRN interacts physically and functionally with the recombination mediator protein RAD52. J Biol Chem. 2003;278:36476-86 pubmed
    ..These results suggest that coordinated WRN and RAD52 activities are involved in replication fork rescue after DNA damage. ..
  13. Machida Y, Kim M, Machida Y. Spartan/C1orf124 is important to prevent UV-induced mutagenesis. Cell Cycle. 2012;11:3395-402 pubmed publisher
    ..Rad18-mediated PCNA ubiquitination is a crucial event that triggers a number of downstream pathways important for lesion bypass...
  14. Haracska L, Johnson R, Unk I, Phillips B, Hurwitz J, Prakash L, et al. Targeting of human DNA polymerase iota to the replication machinery via interaction with PCNA. Proc Natl Acad Sci U S A. 2001;98:14256-61 pubmed
    ..Here, we provide evidence for the physical interaction of hPoliota with proliferating cell nuclear antigen (PCNA), and show that PCNA, together with replication factor C (RFC) and replication protein A (RPA),..
  15. Motegi A, Liaw H, Lee K, Roest H, Maas A, Wu X, et al. Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks. Proc Natl Acad Sci U S A. 2008;105:12411-6 pubmed publisher
    ..In yeast, one branch of PRR involves proliferating cell nuclear antigen (PCNA) polyubiquitination mediated by the Rad5-Ubc13-Mms2 complex that allows bypass of DNA lesion ..
  16. Qin Z, Lu M, Xu X, Hanna M, Shiomi N, Xiao W. DNA-damage tolerance mediated by PCNA*Ub fusions in human cells is dependent on Rev1 but not Polη. Nucleic Acids Res. 2013;41:7356-69 pubmed publisher
    In response to replication-blocking lesions, proliferating cell nuclear antigen (PCNA) can be sequentially ubiquitinated at the K164 residue, leading to two modes of DNA-damage tolerance, namely, translesion DNA synthesis (TLS) and error-..
  17. Ghosal G, Leung J, Nair B, Fong K, Chen J. Proliferating cell nuclear antigen (PCNA)-binding protein C1orf124 is a regulator of translesion synthesis. J Biol Chem. 2012;287:34225-33 pubmed publisher
    DNA damage-induced proliferating cell nuclear antigen (PCNA) ubiquitination serves as the key event mediating post-replication repair...
  18. Li Y, Wang L, Lu C. An E2F site in the 5'-promoter region contributes to serum-dependent up-regulation of the human proliferating cell nuclear antigen gene. FEBS Lett. 2003;544:112-8 pubmed
    The synthesis of proliferating cell nuclear antigen (PCNA) is strictly regulated during the cell cycle...
  19. Moldovan G, Pfander B, Jentsch S. PCNA controls establishment of sister chromatid cohesion during S phase. Mol Cell. 2006;23:723-32 pubmed
    ..Here, we report that in yeast and humans Eco1 is directly physically coupled to the replication protein PCNA, a ring-shaped cofactor of DNA polymerases...
  20. Banks D, Wu M, Higa L, Gavrilova N, Quan J, Ye T, et al. L2DTL/CDT2 and PCNA interact with p53 and regulate p53 polyubiquitination and protein stability through MDM2 and CUL4A/DDB1 complexes. Cell Cycle. 2006;5:1719-29 pubmed
    ..A novel WD40 domain-containing protein, L2DTL/CDT2 and PCNA were identified as proteins associated with CUL4/DDB1 complexes...
  21. Unk I, Hajdu I, Fatyol K, Hurwitz J, Yoon J, Prakash L, et al. Human HLTF functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination. Proc Natl Acad Sci U S A. 2008;105:3768-73 pubmed publisher
    ..ubiquitin-conjugating enzyme complexes and promotes the Lys-63-linked polyubiquitination of proliferating cell nuclear antigen at its Lys-164 residue...
  22. Zhang Z, Zhang Z, Wang H, Zhang G, Hu D, Xiong J, et al. Proliferating cell nuclear antigen binds DNA polymerase-β and mediates 1-methyl-4-phenylpyridinium-induced neuronal death. PLoS ONE. 2014;9:e106669 pubmed publisher
    ..In the present study, we show that both proliferating cell nuclear antigen (PCNA) and DNA pol-β are required for MPP(+)-induced neuronal death...
  23. Ducoux M, Urbach S, Baldacci G, Hubscher U, Koundrioukoff S, Christensen J, et al. Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta. J Biol Chem. 2001;276:49258-66 pubmed
    The subunit that mediates binding of proliferating cell nuclear antigen (PCNA) to human DNA polymerase delta has not been clearly defined...
  24. Hong R, Chakravarti D. The human proliferating Cell nuclear antigen regulates transcriptional coactivator p300 activity and promotes transcriptional repression. J Biol Chem. 2003;278:44505-13 pubmed
    ..p300 is a transcriptional coactivator with protein acetyltransferase activity, and proliferating cell nuclear antigen (PCNA) plays important roles in DNA replication and repair...
  25. Notenboom V, Hibbert R, van Rossum Fikkert S, Olsen J, Mann M, Sixma T. Functional characterization of Rad18 domains for Rad6, ubiquitin, DNA binding and PCNA modification. Nucleic Acids Res. 2007;35:5819-30 pubmed
    Rad18 is a ubiquitin E3 ligase that monoubiquitinates PCNA on stalled replications forks. This allows recruitment of damage-tolerant polymerases for damage bypass and DNA repair...
  26. Abbas T, Shibata E, Park J, Jha S, Karnani N, Dutta A. CRL4(Cdt2) regulates cell proliferation and histone gene expression by targeting PR-Set7/Set8 for degradation. Mol Cell. 2010;40:9-21 pubmed publisher
    ..ubiquitin ligase responsible for Set8 proteolytic degradation in the S phase of the cell cycle, which requires Set8-PCNA interaction...
  27. Gali H, Juhasz S, Mórocz M, Hajdu I, Fatyol K, Szukacsov V, et al. Role of SUMO modification of human PCNA at stalled replication fork. Nucleic Acids Res. 2012;40:6049-59 pubmed publisher
    ..Whereas ubiquitylation of proliferating cell nuclear antigen (PCNA) facilitates damage bypass, modification of yeast PCNA by small ubiquitin-like modifier (SUMO) ..
  28. Oda H, Hübner M, Beck D, Vermeulen M, Hurwitz J, Spector D, et al. Regulation of the histone H4 monomethylase PR-Set7 by CRL4(Cdt2)-mediated PCNA-dependent degradation during DNA damage. Mol Cell. 2010;40:364-76 pubmed publisher
    ..evidence regarding PR-Set7 expression during the cell cycle, the biological relevance of PR-Set7 interaction with PCNA, and its role in the cell...
  29. Riva F, Savio M, Cazzalini O, Stivala L, Scovassi I, Cox L, et al. Distinct pools of proliferating cell nuclear antigen associated to DNA replication sites interact with the p125 subunit of DNA polymerase delta or DNA ligase I. Exp Cell Res. 2004;293:357-67 pubmed
    b>Proliferating cell nuclear antigen (PCNA) plays an essential role in DNA replication, repair, and cell cycle control. PCNA is a homotrimeric ring that, when encircling DNA, is not easily extractable...
  30. Barkley L, Palle K, Durando M, Day T, Gurkar A, Kakusho N, et al. c-Jun N-terminal kinase-mediated Rad18 phosphorylation facilitates Polη recruitment to stalled replication forks. Mol Biol Cell. 2012;23:1943-54 pubmed publisher
    ..Rad18 chaperones DNA polymerase η (Polη) to sites of UV-induced DNA damage and monoubiquitinates proliferating cell nuclear antigen (PCNA), facilitating engagement of Polη with stalled replication forks and promoting translesion ..
  31. Mitra A, Singh N, Singh A, Garg V, Agarwal A, Sharma M, et al. Association of polymorphisms in base excision repair genes with the risk of breast cancer: a case-control study in North Indian women. Oncol Res. 2008;17:127-35 pubmed
    ..XRCC1), rs4989588/T3337A (FEN1), rs4989586/ G3259A (FEN1), rs4989587/C3315T (FEN1), and rs1050525/G6941T (PCNA) with breast cancer susceptibility...
  32. Masuda Y, Suzuki M, Piao J, Gu Y, Tsurimoto T, Kamiya K. Dynamics of human replication factors in the elongation phase of DNA replication. Nucleic Acids Res. 2007;35:6904-16 pubmed
    ..actions of many proteins, including DNA polymerase delta (pol delta), replication factor C (RFC), proliferating cell nuclear antigen (PCNA) and replication protein A...
  33. Abbas T, Sivaprasad U, Terai K, Amador V, Pagano M, Dutta A. PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex. Genes Dev. 2008;22:2496-506 pubmed publisher
    The DNA polymerase delta processivity factor Proliferating Cell Nuclear Antigen (PCNA) promotes the DNA damage-induced degradation of the replication initiation factor Cdt1 via the CRL4(Cdt2) E3 ubiquitin ligase complex...
  34. Yu Y, Cai J, Tu B, Wu L, Zhao Y, Liu X, et al. Proliferating cell nuclear antigen is protected from degradation by forming a complex with MutT Homolog2. J Biol Chem. 2009;284:19310-20 pubmed publisher
    b>Proliferating cell nuclear antigen (PCNA) has been demonstrated to interact with multiple proteins involved in several metabolic pathways such as DNA replication and repair...
  35. Geng L, Huntoon C, Karnitz L. RAD18-mediated ubiquitination of PCNA activates the Fanconi anemia DNA repair network. J Cell Biol. 2010;191:249-57 pubmed publisher
    ..damage-activated E3 ubiquitin ligase, also participates in ID complex activation by ubiquitylating proliferating cell nuclear antigen (PCNA) on Lys164, an event required for the recruitment of FANCL to chromatin...
  36. Rosental B, Brusilovsky M, Hadad U, Oz D, Appel M, Afergan F, et al. Proliferating cell nuclear antigen is a novel inhibitory ligand for the natural cytotoxicity receptor NKp44. J Immunol. 2011;187:5693-702 pubmed publisher
    ..Proliferating cell nuclear Ag (PCNA) is overexpressed in cancer cells. In this study, we show that the NKp44 receptor recognizes PCNA...
  37. Sakurai S, Kitano K, Yamaguchi H, Hamada K, Okada K, Fukuda K, et al. Structural basis for recruitment of human flap endonuclease 1 to PCNA. EMBO J. 2005;24:683-93 pubmed
    Flap endonuclease-1 (FEN1) is a key enzyme for maintaining genomic stability and replication. Proliferating cell nuclear antigen (PCNA) binds FEN1 and stimulates its endonuclease activity...
  38. Masuda Y, Piao J, Kamiya K. DNA replication-coupled PCNA mono-ubiquitination and polymerase switching in a human in vitro system. J Mol Biol. 2010;396:487-500 pubmed publisher
    Translesion DNA synthesis is a mechanism of DNA damage tolerance, and mono-ubiquitination of proliferating cell nuclear antigen (PCNA) is considered to play a key role in regulating the switch from replicative to translesion DNA ..
  39. Groehler A, Lannigan D. A chromatin-bound kinase, ERK8, protects genomic integrity by inhibiting HDM2-mediated degradation of the DNA clamp PCNA. J Cell Biol. 2010;190:575-86 pubmed publisher
    b>Proliferating cell nuclear antigen (PCNA) acts as a scaffold, coordinator, and stimulator of numerous processes required for faithful transmission of genetic information...
  40. Langie S, Knaapen A, Ramaekers C, Theys J, Brun J, Godschalk R, et al. Formation of lysine 63-linked poly-ubiquitin chains protects human lung cells against benzo[a]pyrene-diol-epoxide-induced mutagenicity. DNA Repair (Amst). 2007;6:852-62 pubmed
    ..Studies in yeast suggest the modification of PCNA by lysine 63-linked poly-ubiquitin (K63-polyUb) chains as a key mediator of the error-free damage avoidance pathway...
  41. Hervouet E, Lalier L, Debien E, Cheray M, Geairon A, Rogniaux H, et al. Disruption of Dnmt1/PCNA/UHRF1 interactions promotes tumorigenesis from human and mice glial cells. PLoS ONE. 2010;5:e11333 pubmed publisher
    ..Here, we show that the disruption of Dnmt1/PCNA/UHRF1 interactions promotes a global DNA hypomethylation in human gliomas...
  42. Fox J, Lee K, Myung K. Dynamic regulation of PCNA ubiquitylation/deubiquitylation. FEBS Lett. 2011;585:2780-5 pubmed publisher
    b>Proliferating Cell Nuclear Antigen (PCNA) ubiquitylation plays a crucial role in maintaining genomic stability during DNA replication...
  43. Masuda Y, Suzuki M, Kawai H, Suzuki F, Kamiya K. Asymmetric nature of two subunits of RAD18, a RING-type ubiquitin ligase E3, in the human RAD6A-RAD18 ternary complex. Nucleic Acids Res. 2012;40:1065-76 pubmed publisher
    ..These results suggest an asymmetric nature of the two RAD18 subunits in the complex. ..
  44. Zlatanou A, Despras E, Braz Petta T, Boubakour Azzouz I, Pouvelle C, Stewart G, et al. The hMsh2-hMsh6 complex acts in concert with monoubiquitinated PCNA and Pol ? in response to oxidative DNA damage in human cells. Mol Cell. 2011;43:649-62 pubmed publisher
    Posttranslational modification of PCNA by ubiquitin plays an important role in coordinating the processes of DNA damage tolerance during DNA replication...
  45. Juhasz S, Balogh D, Hajdu I, Burkovics P, Villamil M, Zhuang Z, et al. Characterization of human Spartan/C1orf124, an ubiquitin-PCNA interacting regulator of DNA damage tolerance. Nucleic Acids Res. 2012;40:10795-808 pubmed publisher
    ..Replication through DNA lesions depends on mono- and polyubiquitylation of proliferating cell nuclear antigen (PCNA), which enable translesion synthesis (TLS) and template switching, respectively...
  46. Lu X, Tan C, Zhou J, You M, Carastro L, Downey K, et al. Direct interaction of proliferating cell nuclear antigen with the small subunit of DNA polymerase delta. J Biol Chem. 2002;277:24340-5 pubmed
    The interaction between proliferating cell nuclear antigen (PCNA) and DNA polymerase delta is essential for processive DNA synthesis during DNA replication/repair; however, the identity of the subunit of DNA polymerase delta that directly ..
  47. Mosbech A, Gibbs Seymour I, Kagias K, Thorslund T, Beli P, Povlsen L, et al. DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks. Nat Struct Mol Biol. 2012;19:1084-92 pubmed publisher
    ..DVC1 recruitment to sites of replication stress requires its ubiquitin-binding UBZ domain and PCNA-binding PIP box motif but is independent of RAD18-mediated PCNA monoubiquitylation...
  48. Tomida J, Masuda Y, Hiroaki H, Ishikawa T, Song I, Tsurimoto T, et al. DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex. J Biol Chem. 2008;283:9071-9 pubmed publisher
    ..b>Proliferating cell nuclear antigen (PCNA; a homotrimeric protein that encircles double-stranded DNA to function as a sliding clamp for ..
  49. Henneke G, Koundrioukoff S, Hubscher U. Phosphorylation of human Fen1 by cyclin-dependent kinase modulates its role in replication fork regulation. Oncogene. 2003;22:4301-13 pubmed
    ..Moreover, phosphorylation of Fen1 by Cdk1-Cyclin A abrogates its proliferating cell nuclear antigen (PCNA) binding thus preventing stimulation of Fen1 by PCNA...
  50. Cazzalini O, Perucca P, Savio M, Necchi D, Bianchi L, Stivala L, et al. Interaction of p21(CDKN1A) with PCNA regulates the histone acetyltransferase activity of p300 in nucleotide excision repair. Nucleic Acids Res. 2008;36:1713-22 pubmed publisher
    ..inhibitor p21(CDKN1A) has been suggested to directly participate in DNA repair, thanks to the interaction with PCNA. Yet, its role has remained unclear...
  51. Song I, Palle K, Gurkar A, Tateishi S, Kupfer G, Vaziri C. Rad18-mediated translesion synthesis of bulky DNA adducts is coupled to activation of the Fanconi anemia DNA repair pathway. J Biol Chem. 2010;285:31525-36 pubmed publisher
    ..ligase Rad18 (a central regulator of TLS) induces DNA damage-independent mono-ubiquitination of proliferating cell nuclear antigen (PCNA) (a known Rad18 substrate) and FANCD2...
  52. Jones M, Colnaghi L, Huang T. Dysregulation of DNA polymerase ? recruitment to replication forks results in genomic instability. EMBO J. 2012;31:908-18 pubmed publisher
    ..Moreover, artificial tethering of Pol? to proliferating cell nuclear antigen (PCNA) circumvents the need for its ubiquitin-binding domain in the promotion of genomic instability...
  53. Weston R, Peeters H, Ahel D. ZRANB3 is a structure-specific ATP-dependent endonuclease involved in replication stress response. Genes Dev. 2012;26:1558-72 pubmed publisher
    ..It is recruited to damaged replication forks via multiple mechanisms, which involve interactions with PCNA, K63-polyubiquitin chains, and branched DNA structures...
  54. Vieyra D, Loewith R, Scott M, Bonnefin P, Boisvert F, Cheema P, et al. Human ING1 proteins differentially regulate histone acetylation. J Biol Chem. 2002;277:29832-9 pubmed
    ..we present evidence indicating that p33(ING1b) affects the degree of physical association between proliferating cell nuclear antigen (PCNA) and p300, an association that has been proposed to link DNA repair to chromatin remodeling...
  55. Bienko M, Green C, Crosetto N, Rudolf F, Zapart G, Coull B, et al. Ubiquitin-binding domains in Y-family polymerases regulate translesion synthesis. Science. 2005;310:1821-4 pubmed
    ..Upon DNA damage, ubiquitination of proliferating cell nuclear antigen (PCNA) induces bypass of the lesion by directing the replication machinery into the TLS pathway...
  56. Chandrasekaran S, Tan T, Hall J, Cook J. Stress-stimulated mitogen-activated protein kinases control the stability and activity of the Cdt1 DNA replication licensing factor. Mol Cell Biol. 2011;31:4405-16 pubmed publisher
    ..Two other CRL4(Cdt2) targets, the cyclin-dependent kinase (CDK) inhibitor p21 and the methyltransferase PR-Set7/Set8, are similarly stabilized by MAP ..
  57. Kannouche P, Wing J, Lehmann A. Interaction of human DNA polymerase eta with monoubiquitinated PCNA: a possible mechanism for the polymerase switch in response to DNA damage. Mol Cell. 2004;14:491-500 pubmed
    ..We show that, in human cells, PCNA becomes monoubiquitinated following UV irradiation of the cells and that this is dependent on the hRad18 protein...
  58. Bi X, Barkley L, Slater D, Tateishi S, Yamaizumi M, Ohmori H, et al. Rad18 regulates DNA polymerase kappa and is required for recovery from S-phase checkpoint-mediated arrest. Mol Cell Biol. 2006;26:3527-40 pubmed
    ..The DNA polymerase processivity factor PCNA is monoubiquitinated and interacts with Polkappa in cells treated with the bulky adduct-forming genotoxin benzo[a]..
  59. Wang S, Nakajima Y, Yu Y, Xia W, Chen C, Yang C, et al. Tyrosine phosphorylation controls PCNA function through protein stability. Nat Cell Biol. 2006;8:1359-68 pubmed
    The proliferating cell nuclear antigen (PCNA) is an essential protein for DNA replication and damage repair. How its function is controlled remains an important question...
  60. Zhang S, Chea J, Meng X, Zhou Y, Lee E, Lee M. PCNA is ubiquitinated by RNF8. Cell Cycle. 2008;7:3399-404 pubmed
    The ubiquitination of PCNA is an essential event in the operation of the DNA Damage Tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase...
  61. Punchihewa C, Inoue A, Hishiki A, Fujikawa Y, Connelly M, Evison B, et al. Identification of small molecule proliferating cell nuclear antigen (PCNA) inhibitor that disrupts interactions with PIP-box proteins and inhibits DNA replication. J Biol Chem. 2012;287:14289-300 pubmed publisher
    ..We have discovered that 3,3',5-triiodothyronine (T3) inhibits binding of a PIP-box sequence peptide to proliferating cell nuclear antigen (PCNA) protein by competing for the same binding site, as evidenced by the co-crystal structure of ..
  62. Durando M, Tateishi S, Vaziri C. A non-catalytic role of DNA polymerase η in recruiting Rad18 and promoting PCNA monoubiquitination at stalled replication forks. Nucleic Acids Res. 2013;41:3079-93 pubmed publisher
    ..TLS is initiated when the Rad6/Rad18 complex monoubiquitinates proliferating cell nuclear antigen (PCNA), but the basis for recruitment of Rad18 to PCNA is not completely understood...
  63. Terai K, Shibata E, Abbas T, Dutta A. Degradation of p12 subunit by CRL4Cdt2 E3 ligase inhibits fork progression after DNA damage. J Biol Chem. 2013;288:30509-14 pubmed publisher
    ..These results suggest that ubiquitination of p12 through CRL4(Cdt2) and subsequent degradation form one mechanism by which a cell responds to DNA damage to inhibit fork progression. ..
  64. Guo C, Tang T, Bienko M, Parker J, Bielen A, Sonoda E, et al. Ubiquitin-binding motifs in REV1 protein are required for its role in the tolerance of DNA damage. Mol Cell Biol. 2006;26:8892-900 pubmed
    ..The UBMs also mediate the enhanced association between monoubiquitylated PCNA and REV1...
  65. Acharya N, Yoon J, Gali H, Unk I, Haracska L, Johnson R, et al. Roles of PCNA-binding and ubiquitin-binding domains in human DNA polymerase eta in translesion DNA synthesis. Proc Natl Acad Sci U S A. 2008;105:17724-9 pubmed publisher
    ..of yeast and human cells with DNA-damaging agents elicits Rad6-Rad18-mediated monoubiquitination of proliferating cell nuclear antigen (PCNA) at its Lys-164 residue [ubiquitin (Ub)-PCNA], and this PCNA modification is indispensable for ..
  66. Havens C, Shobnam N, Guarino E, Centore R, Zou L, Kearsey S, et al. Direct role for proliferating cell nuclear antigen in substrate recognition by the E3 ubiquitin ligase CRL4Cdt2. J Biol Chem. 2012;287:11410-21 pubmed publisher
    ..CRL4(Cdt2) substrates contain a "PIP degron," which consists of a canonical proliferating cell nuclear antigen (PCNA) interaction motif (PIP box) and an adjacent basic amino acid...
  67. Scott M, Bonnefin P, Vieyra D, Boisvert F, Young D, Bazett Jones D, et al. UV-induced binding of ING1 to PCNA regulates the induction of apoptosis. J Cell Sci. 2001;114:3455-62 pubmed
    Previous studies have shown that UV-induced binding of p21(WAF1) to PCNA through the PCNA-interacting protein (PIP) domain in p21(WAF1) promotes a switch from DNA replication to DNA repair by altering the PCNA protein complex...
  68. Kontopidis G, Wu S, Zheleva D, Taylor P, McInnes C, Lane D, et al. Structural and biochemical studies of human proliferating cell nuclear antigen complexes provide a rationale for cyclin association and inhibitor design. Proc Natl Acad Sci U S A. 2005;102:1871-6 pubmed
    ..the tumor suppressor protein p21WAF1 and the cyclin-dependent kinase (CDK) complexes and with proliferating cell nuclear antigen (PCNA) regulate and coordinate the processes of cell-cycle progression and DNA replication...
  69. Perucca P, Cazzalini O, Mortusewicz O, Necchi D, Savio M, Nardo T, et al. Spatiotemporal dynamics of p21CDKN1A protein recruitment to DNA-damage sites and interaction with proliferating cell nuclear antigen. J Cell Sci. 2006;119:1517-27 pubmed
    ..by inducing cell-cycle arrest, and by inhibiting DNA replication through association with the proliferating cell nuclear antigen (PCNA)...
  70. Larrieu D, Ythier D, Binet R, Brambilla C, Brambilla E, Sengupta S, et al. ING2 controls the progression of DNA replication forks to maintain genome stability. EMBO Rep. 2009;10:1168-74 pubmed publisher
    ..Accordingly, we found that ING2 interacts with proliferating cell nuclear antigen and regulates its amount to the chromatin fraction, allowing normal replication progression and ..
  71. Jung Y, Hakem A, Hakem R, Chen X. Pirh2 E3 ubiquitin ligase monoubiquitinates DNA polymerase eta to suppress translesion DNA synthesis. Mol Cell Biol. 2011;31:3997-4006 pubmed publisher
    ..We also show that monoubiquitination of PolH inhibits the ability of PolH to interact with PCNA and to bypass UV-induced lesions, leading to decreased viability of UV-damaged cells...
  72. Masuda Y, Suzuki M, Kawai H, Hishiki A, Hashimoto H, Masutani C, et al. En bloc transfer of polyubiquitin chains to PCNA in vitro is mediated by two different human E2-E3 pairs. Nucleic Acids Res. 2012;40:10394-407 pubmed publisher
    Post-replication DNA repair in eukaryotes is regulated by ubiquitination of proliferating cell nuclear antigen (PCNA)...
  73. Sebesta M, Burkovics P, Juhasz S, Zhang S, Szabó J, Lee M, et al. Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans. DNA Repair (Amst). 2013;12:691-8 pubmed publisher
    ..In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a ..
  74. Maga G, Villani G, Ramadan K, Shevelev I, Tanguy Le Gac N, Blanco L, et al. Human DNA polymerase lambda functionally and physically interacts with proliferating cell nuclear antigen in normal and translesion DNA synthesis. J Biol Chem. 2002;277:48434-40 pubmed
    b>Proliferating cell nuclear antigen (PCNA) has been shown to interact with a variety of DNA polymerases (pol) such as pol delta, pol epsilon, pol iota, pol kappa, pol eta, and pol beta...
  75. Senga T, Sivaprasad U, Zhu W, Park J, Arias E, Walter J, et al. PCNA is a cofactor for Cdt1 degradation by CUL4/DDB1-mediated N-terminal ubiquitination. J Biol Chem. 2006;281:6246-52 pubmed
    ..that is active after DNA damage and in S-phase and is dependent on the interaction of Cdt1 with proliferating cell nuclear antigen (PCNA) through a PCNA binding motif...
  76. Simpson L, Ross A, Szuts D, Alviani C, Oestergaard V, Patel K, et al. RAD18-independent ubiquitination of proliferating-cell nuclear antigen in the avian cell line DT40. EMBO Rep. 2006;7:927-32 pubmed
    Ubiquitination of proliferating-cell nuclear antigen (PCNA) at K164 by RAD6/RAD18 has a key role in DNA damage tolerance in yeast. Here, we report on the first genetic study of this modification in a vertebrate cell...
  77. Unk I, Hajdu I, Fatyol K, Szakal B, Blastyak A, Bermudez V, et al. Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen. Proc Natl Acad Sci U S A. 2006;103:18107-12 pubmed
    ..a role for Rad5 as a ubiquitin ligase in mediating the Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen. Here we show that SHPRH is a functional homolog of Rad5...
  78. Witko Sarsat V, Mocek J, Bouayad D, Tamassia N, Ribeil J, Candalh C, et al. Proliferating cell nuclear antigen acts as a cytoplasmic platform controlling human neutrophil survival. J Exp Med. 2010;207:2631-45 pubmed publisher
    ..In this study, we describe a survival pathway controlled by proliferating cell nuclear antigen (PCNA), a nuclear factor involved in DNA replication and repairing of proliferating cells...
  79. Miotto B, Struhl K. JNK1 phosphorylation of Cdt1 inhibits recruitment of HBO1 histone acetylase and blocks replication licensing in response to stress. Mol Cell. 2011;44:62-71 pubmed publisher
  80. Hibbert R, Sixma T. Intrinsic flexibility of ubiquitin on proliferating cell nuclear antigen (PCNA) in translesion synthesis. J Biol Chem. 2012;287:39216-23 pubmed publisher
    ..One important substrate is monoubiquitinated PCNA (PCNA-Ub), which signals for recruitment of damage-tolerant polymerases in the translesion synthesis (TLS) pathway ..
  81. Davis E, Lachaud C, Appleton P, MacArtney T, Näthke I, Rouse J. DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage. Nat Struct Mol Biol. 2012;19:1093-100 pubmed publisher
    ..in a manner that requires the ability of the DVC1 UBZ domain to bind to ubiquitin polymers in vitro and a conserved PCNA-interacting motif. DVC1 interacts with the p97 protein 'segregase'...
  82. Easwaran H, Schermelleh L, Leonhardt H, Cardoso M. Replication-independent chromatin loading of Dnmt1 during G2 and M phases. EMBO Rep. 2004;5:1181-6 pubmed
  83. Naryzhny S, Zhao H, Lee H. Proliferating cell nuclear antigen (PCNA) may function as a double homotrimer complex in the mammalian cell. J Biol Chem. 2005;280:13888-94 pubmed
    The diverse function of proliferating cell nuclear antigen (PCNA) may be regulated by interactions with different protein partners...
  84. Jørgensen S, Elvers I, Trelle M, Menzel T, Eskildsen M, Jensen O, et al. The histone methyltransferase SET8 is required for S-phase progression. J Cell Biol. 2007;179:1337-45 pubmed publisher
    ..Furthermore, we find that SET8 interacts with proliferating cell nuclear antigen through a conserved motif, and SET8 is required for DNA replication fork progression...
  85. Niimi A, Brown S, Sabbioneda S, Kannouche P, Scott A, Yasui A, et al. Regulation of proliferating cell nuclear antigen ubiquitination in mammalian cells. Proc Natl Acad Sci U S A. 2008;105:16125-30 pubmed publisher
    ..to DNA-damaging agents that block the progress of the replication fork, monoubiquitination of proliferating cell nuclear antigen (PCNA) mediates the switch from replicative to translesion synthesis DNA polymerases...
  86. Freudenthal B, Gakhar L, Ramaswamy S, Washington M. Structure of monoubiquitinated PCNA and implications for translesion synthesis and DNA polymerase exchange. Nat Struct Mol Biol. 2010;17:479-84 pubmed publisher
    ..In eukaryotes this polymerase exchange requires proliferating cell nuclear antigen (PCNA) monoubiquitination...
  87. Zhang W, Qin Z, Zhang X, Xiao W. Roles of sequential ubiquitination of PCNA in DNA-damage tolerance. FEBS Lett. 2011;585:2786-94 pubmed publisher
    ..budding yeast is achieved through sequential ubiquitination of DNA polymerase processivity factor, proliferating cell nuclear antigen (PCNA)...
  88. Lehmann A. Ubiquitin-family modifications in the replication of DNA damage. FEBS Lett. 2011;585:2772-9 pubmed publisher
    ..All the Y-family polymerases have ubiquitin binding domains that bind to mono-ubiquitinated PCNA to effect the switching from replicative to Y-family polymerase...
  89. Zhang Z, Zhang S, Lin S, Wang X, Wu L, Lee E, et al. Structure of monoubiquitinated PCNA: implications for DNA polymerase switching and Okazaki fragment maturation. Cell Cycle. 2012;11:2128-36 pubmed publisher
    Ubiquitination of proliferating cell nuclear antigen (PCNA) to ub-PCNA is essential for DNA replication across bulky template lesions caused by UV radiation and alkylating agents, as ub-PCNA orchestrates the recruitment and switching of ..
  90. Centore R, Yazinski S, Tse A, Zou L. Spartan/C1orf124, a reader of PCNA ubiquitylation and a regulator of UV-induced DNA damage response. Mol Cell. 2012;46:625-35 pubmed publisher
    b>PCNA is a key component of DNA replication and repair machineries. DNA damage-induced PCNA ubiquitylation serves as a molecular mark to orchestrate postreplication repair...
  91. Haracska L, Unk I, Johnson R, Phillips B, Hurwitz J, Prakash L, et al. Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA. Mol Cell Biol. 2002;22:784-91 pubmed
    ..Here, we provide evidence for the physical interaction of human Polkappa (hPolkappa) with proliferating cell nuclear antigen (PCNA) and show that PCNA, replication factor C (RFC), and replication protein A (RPA) act ..
  92. Xie B, Mazloum N, Liu L, Rahmeh A, Li H, Lee M. Reconstitution and characterization of the human DNA polymerase delta four-subunit holoenzyme. Biochemistry. 2002;41:13133-42 pubmed
    ..The heterodimer is only weakly stimulated by PCNA, whereas the heterotetramer is strongly stimulated to a level with a specific activity comparable to that of the ..