PCBP2

Summary

Gene Symbol: PCBP2
Description: poly(rC) binding protein 2
Alias: HNRNPE2, HNRPE2, hnRNP-E2, poly(rC)-binding protein 2, alpha-CP2, heterogeneous nuclear ribonucleoprotein E2, heterogenous nuclear ribonucleoprotein E2, hnRNP E2
Species: human
Products:     PCBP2

Top Publications

  1. Kiledjian M, Wang X, Liebhaber S. Identification of two KH domain proteins in the alpha-globin mRNP stability complex. EMBO J. 1995;14:4357-64 pubmed
    ..As such this represents the first example of a specific function for this class of proteins and suggests potential roles for other members of this protein family. ..
  2. Dinh P, Beura L, Panda D, Das A, Pattnaik A. Antagonistic effects of cellular poly(C) binding proteins on vesicular stomatitis virus gene expression. J Virol. 2011;85:9459-71 pubmed publisher
    ..from cells expressing the vesicular stomatitis virus (VSV) P protein identified the poly(C) binding protein 2 (PCBP2) as one of the P protein-interacting proteins...
  3. Blyn L, Swiderek K, Richards O, Stahl D, Semler B, Ehrenfeld E. Poly(rC) binding protein 2 binds to stem-loop IV of the poliovirus RNA 5' noncoding region: identification by automated liquid chromatography-tandem mass spectrometry. Proc Natl Acad Sci U S A. 1996;93:11115-20 pubmed
    ..The sequence obtained matched that of poly(rC) binding protein 2 (PCBP2), previously identified as an RNA binding protein from human cells...
  4. Spear A, Sharma N, Flanegan J. Protein-RNA tethering: the role of poly(C) binding protein 2 in poliovirus RNA replication. Virology. 2008;374:280-91 pubmed publisher
    ..Poliovirus utilizes a multifunctional cellular protein, poly(C) binding protein 2 (PCBP2), for RNA stability, translation and RNA replication...
  5. Du Z, Lee J, Tjhen R, Li S, Pan H, Stroud R, et al. Crystal structure of the first KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.7 A. J Biol Chem. 2005;280:38823-30 pubmed
    ..7-A resolution, of PCBP2 KH1 in complex with a 7-nucleotide DNA sequence (5'-AACCCTA-3') corresponding to one repeat of the human C-rich ..
  6. Du Z, Yu J, Chen Y, Andino R, James T. Specific recognition of the C-rich strand of human telomeric DNA and the RNA template of human telomerase by the first KH domain of human poly(C)-binding protein-2. J Biol Chem. 2004;279:48126-34 pubmed
    ..We have determined the NMR structure of the first KH domain (KH1) from PCBP2. The PCBP2 KH1 domain adopts a structure with three alpha-helices packed against one side of a three-stranded ..
  7. Sean P, Nguyen J, Semler B. The linker domain of poly(rC) binding protein 2 is a major determinant in poliovirus cap-independent translation. Virology. 2008;378:243-53 pubmed publisher
    ..Previous studies have shown that binding of a cellular protein, poly(rC) binding protein 2 (PCBP2), to a major stem-loop structure in the genomic 5' NCR is necessary for the translation of picornaviruses ..
  8. Makeyev A, Chkheidze A, Liebhaber S. A set of highly conserved RNA-binding proteins, alphaCP-1 and alphaCP-2, implicated in mRNA stabilization, are coexpressed from an intronless gene and its intron-containing paralog. J Biol Chem. 1999;274:24849-57 pubmed
  9. Yeap B, Voon D, Vivian J, McCulloch R, Thomson A, Giles K, et al. Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA. J Biol Chem. 2002;277:27183-92 pubmed
    ..The binding of HuR, CP1, and CP2 to AR mRNA suggests a role for each of these proteins in the post-transcriptional regulation of AR expression in cancer cells. ..

More Information

Publications100

  1. Du Z, Fenn S, Tjhen R, James T. Structure of a construct of a human poly(C)-binding protein containing the first and second KH domains reveals insights into its regulatory mechanisms. J Biol Chem. 2008;283:28757-66 pubmed publisher
    ..and function, we determined the structure of a construct containing the first two domains (KH1-KH2) of human PCBP2 by NMR. KH1 and KH2 form an intramolecular pseudodimer...
  2. Waggoner S, Johannes G, Liebhaber S. Depletion of the poly(C)-binding proteins alphaCP1 and alphaCP2 from K562 cells leads to p53-independent induction of cyclin-dependent kinase inhibitor (CDKN1A) and G1 arrest. J Biol Chem. 2009;284:9039-49 pubmed publisher
    ..These data reveal a pivotal role for alphaCP1 and alphaCP2 in a p53-independent pathway of p21(WAF) control and cell cycle progression. ..
  3. You F, Sun H, Zhou X, Sun W, Liang S, Zhai Z, et al. PCBP2 mediates degradation of the adaptor MAVS via the HECT ubiquitin ligase AIP4. Nat Immunol. 2009;10:1300-8 pubmed publisher
    ..MAVS regulation is essential for the prevention of excessive harmful immune responses. Here we identify PCBP2 as a negative regulator in MAVS-mediated signaling...
  4. Czyzyk Krzeska M, Bendixen A. Identification of the poly(C) binding protein in the complex associated with the 3' untranslated region of erythropoietin messenger RNA. Blood. 1999;93:2111-20 pubmed
    ..we found that two isoforms of a 40 kD poly(C) binding protein (PCBP, also known as alphaCP or hnRNPE), PCBP1, and PCBP2 are present in that complex...
  5. Fujimura K, Katahira J, Kano F, Yoneda Y, Murata M. Selective localization of PCBP2 to cytoplasmic processing bodies. Biochim Biophys Acta. 2009;1793:878-87 pubmed publisher
    ..Previously, we reported that PCBP2 (Poly-(rC) Binding Protein 2), a facilitator of IRES-mediated translation, is a novel P-body component...
  6. Walter B, Parsley T, Ehrenfeld E, Semler B. Distinct poly(rC) binding protein KH domain determinants for poliovirus translation initiation and viral RNA replication. J Virol. 2002;76:12008-22 pubmed
    ..protein that plays a role in both translation initiation and viral RNA synthesis is poly(rC) binding protein 2 (PCBP2)...
  7. Wang Z, Day N, Trifillis P, Kiledjian M. An mRNA stability complex functions with poly(A)-binding protein to stabilize mRNA in vitro. Mol Cell Biol. 1999;19:4552-60 pubmed
    ..Therefore, the alpha-complex is an mRNA stability complex in vitro which could function at least in part by interacting with PABP. ..
  8. Blyn L, Towner J, Semler B, Ehrenfeld E. Requirement of poly(rC) binding protein 2 for translation of poliovirus RNA. J Virol. 1997;71:6243-6 pubmed
    Poly(rC) binding protein 2 (PCBP2) is one of several cellular proteins that interact specifically with a major stem-loop domain in the poliovirus internal ribosome entry site...
  9. Roychoudhury P, Paul R, Chowdhury R, Chaudhuri K. HnRNP E2 is downregulated in human oral cancer cells and the overexpression of hnRNP E2 induces apoptosis. Mol Carcinog. 2007;46:198-207 pubmed
    ..The present study investigated the clinicopathologic and biological significance of hnRNP E2 gene expression in oral cancer...
  10. Fenn S, Du Z, Lee J, Tjhen R, Stroud R, James T. Crystal structure of the third KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.6 A resolution. Nucleic Acids Res. 2007;35:2651-60 pubmed
    ..6 A resolution, of PCBP2 KH3 domain in complex with a 7-nt DNA sequence (5'-AACCCTA-3') corresponding to one repeat of the C-rich strand of ..
  11. Du Z, Lee J, Fenn S, Tjhen R, Stroud R, James T. X-ray crystallographic and NMR studies of protein-protein and protein-nucleic acid interactions involving the KH domains from human poly(C)-binding protein-2. RNA. 2007;13:1043-51 pubmed
    ..of KH domain-D/RNA recognition and its functional importance, we have determined the crystal structures of PCBP2 KH1 domain in complex with a 12-nucleotide DNA corresponding to two repeats of the human C-rich strand telomeric ..
  12. Ogram S, Spear A, Sharma N, Flanegan J. The 5'CL-PCBP RNP complex, 3' poly(A) tail and 2A(pro) are required for optimal translation of poliovirus RNA. Virology. 2010;397:14-22 pubmed publisher
    ..that the 5'CL-PCBP complex is required for optimal viral RNA translation and identified the KH3 domain of PCBP2 as the functional region...
  13. Ghosh D, Srivastava G, Xu D, Schulz L, Roberts R. A link between SIN1 (MAPKAP1) and poly(rC) binding protein 2 (PCBP2) in counteracting environmental stress. Proc Natl Acad Sci U S A. 2008;105:11673-8 pubmed publisher
    ..a two-hybrid screen of a human bone marrow cDNA library, its most frequent partner was poly(rC) binding protein 2 (PCBP2/hnRNP-E2), which associates with the N-terminal domain of SIN1 and can be coimmunoprecipitated with SIN1 and the ..
  14. Wang L, Jeng K, Lai M. Poly(C)-binding protein 2 interacts with sequences required for viral replication in the hepatitis C virus (HCV) 5' untranslated region and directs HCV RNA replication through circularizing the viral genome. J Virol. 2011;85:7954-64 pubmed publisher
    ..We also found that poly(C)-binding protein 2 (PCBP2) bound to the replication region of the 5'UTR and associated with detergent-resistant membrane fractions, which ..
  15. Fujimura K, Kano F, Murata M. Identification of PCBP2, a facilitator of IRES-mediated translation, as a novel constituent of stress granules and processing bodies. RNA. 2008;14:425-31 pubmed publisher
    ..In this study, we report that PCBP2, a facilitator of IRES (Internal Ribosomal Entry Site)-mediated translation, is a novel constituent of the SG and ..
  16. Perera R, Daijogo S, Walter B, Nguyen J, Semler B. Cellular protein modification by poliovirus: the two faces of poly(rC)-binding protein. J Virol. 2007;81:8919-32 pubmed
    ..Among the four different isoforms of PCBP in mammalian cells, PCBP2 is required for translation initiation on picornavirus genomes with type I internal ribosome entry site elements ..
  17. Eiring A, Harb J, Neviani P, Garton C, Oaks J, Spizzo R, et al. miR-328 functions as an RNA decoy to modulate hnRNP E2 regulation of mRNA translation in leukemic blasts. Cell. 2010;140:652-65 pubmed publisher
    ..crisis chronic myelogenous leukemia (CML-BC) in a BCR/ABL dose- and kinase-dependent manner through the MAPK-hnRNP E2 pathway...
  18. Smirnova V, Shestakova E, Bikmetov D, Chugunova A, Osterman I, Serebryakova M, et al. eIF4G2 balances its own mRNA translation via a PCBP2-based feedback loop. RNA. 2019;: pubmed publisher
    Poly(rC)-binding protein 2 (PCBP2, hnRNP E2) is one of the most abundant RNA-binding proteins in mammalian cells. In humans, it exists in seven isoforms, which are assumed to play similar roles in cells...
  19. Ghanem L, Kromer A, Silverman I, Ji X, Gazzara M, Nguyen N, et al. Poly(C)-Binding Protein Pcbp2 Enables Differentiation of Definitive Erythropoiesis by Directing Functional Splicing of the Runx1 Transcript. Mol Cell Biol. 2018;38: pubmed publisher
    ..Here, we report that mouse embryos lacking the KH domain poly(C) binding protein, Pcbp2, are selectively deficient in the definitive erythroid lineage...
  20. Zhang H, Song L, Cong H, Tien P. Nuclear Protein Sam68 Interacts with the Enterovirus 71 Internal Ribosome Entry Site and Positively Regulates Viral Protein Translation. J Virol. 2015;89:10031-43 pubmed publisher
    ..from the nucleus to the cytoplasm and interacts with cellular factors, such as poly(rC)-binding protein 2 (PCBP2) and poly(A)-binding protein (PABP), during EV71 infection...
  21. Shi C, Qi H, Boularan C, Huang N, Abu Asab M, Shelhamer J, et al. SARS-coronavirus open reading frame-9b suppresses innate immunity by targeting mitochondria and the MAVS/TRAF3/TRAF6 signalosome. J Immunol. 2014;193:3080-9 pubmed publisher
    ..acting on mitochondria, ORF-9b targets the mitochondrial-associated adaptor molecule MAVS signalosome by usurping PCBP2 and the HECT domain E3 ligase AIP4 to trigger the degradation of MAVS, TRAF3, and TRAF 6...
  22. Zhang Y, Si Y, Ma N, Mei J. The RNA-binding protein PCBP2 inhibits Ang II-induced hypertrophy of cardiomyocytes though promoting GPR56 mRNA degeneration. Biochem Biophys Res Commun. 2015;464:679-84 pubmed publisher
    ..However, their physiological or pathological functions in the hearts remain unknown. Here we reported that PCBP2 is an anti-hypertrophic factor by inhibiting GPR56 mRNA stability...
  23. Han W, Xin Z, Zhao Z, Bao W, Lin X, Yin B, et al. RNA-binding protein PCBP2 modulates glioma growth by regulating FHL3. J Clin Invest. 2013;123:2103-18 pubmed publisher
    b>PCBP2 is a member of the poly(C)-binding protein (PCBP) family, which plays an important role in posttranscriptional and translational regulation by interacting with single-stranded poly(C) motifs in target mRNAs...
  24. Morrow J, Cho M, Platig J, Zhou X, DeMeo D, Qiu W, et al. Ensemble genomic analysis in human lung tissue identifies novel genes for chronic obstructive pulmonary disease. Hum Genomics. 2018;12:1 pubmed publisher
    ..The genes HNRNPAB and PCBP2 with RNA binding activity and the gene ACVR1B were identified in network communities with validated disease ..
  25. Gerbasi V, Link A. The myotonic dystrophy type 2 protein ZNF9 is part of an ITAF complex that promotes cap-independent translation. Mol Cell Proteomics. 2007;6:1049-58 pubmed
    ..One of these RNA-binding proteins, PCBP2, is an established activator of viral and cellular IRESs...
  26. Dhaenens L, Lierman S, De Clerck L, Govaert E, Deforce D, Tilleman K, et al. Endometrial stromal cell proteome mapping in repeated implantation failure and recurrent pregnancy loss cases and fertile women. Reprod Biomed Online. 2019;38:442-454 pubmed publisher
    ..samples in the different groups revealed six differentially expressed proteins: DUX4L2, CNPY4, PDE7A, CTSK, PCBP2 and PSMD4...
  27. Asnani M, Pestova T, Hellen C. PCBP2 enables the cadicivirus IRES to exploit the function of a conserved GRNA tetraloop to enhance ribosomal initiation complex formation. Nucleic Acids Res. 2016;44:9902-9917 pubmed
    ..Type 1 and divergent cadicivirus IRESs require the same IRES trans-acting factor, poly(C)-binding protein 2 (PCBP2). PCBP2 has three KH domains and binds poliovirus IRES domain dIV in the vicinity of the tetraloop...
  28. López Manríquez E, Vashist S, Ureña L, Goodfellow I, Chavez P, Mora Heredia J, et al. Norovirus genome circularization and efficient replication are facilitated by binding of PCBP2 and hnRNP A1. J Virol. 2013;87:11371-87 pubmed publisher
    ..Extracts treated with antibodies to poly(C) binding protein 2 (PCBP2) and heterogeneous nuclear ribonucleoprotein (hnRNP) A1 significantly reduced the 5'-3' interaction...
  29. Masaki T, Arend K, Li Y, Yamane D, McGivern D, Kato T, et al. miR-122 stimulates hepatitis C virus RNA synthesis by altering the balance of viral RNAs engaged in replication versus translation. Cell Host Microbe. 2015;17:217-28 pubmed publisher
    ..Additionally, miR-122 reduces the fraction of viral genomes engaged in protein translation. Depleting AGO2 or PCBP2, which binds HCV RNA in competition with miR-122 and promotes translation, eliminates miR-122 stimulation of ..
  30. Yanatori I, Kishi F. DMT1 and iron transport. Free Radic Biol Med. 2019;133:55-63 pubmed publisher
    ..We summarize DMT1 expression depending on the types of cell or tissue and the function and mechanism of one of the iron chaperones, PCBP2.
  31. Lin X, Yang B, Liu W, Tan X, Wu F, Hu P, et al. Interplay between PCBP2 and miRNA modulates ARHGDIA expression and function in glioma migration and invasion. Oncotarget. 2016;7:19483-98 pubmed publisher
    ..The RNA-binding protein PCBP2, an oncogenic protein in human malignant gliomas, is an essential regulator of mRNA and miRNA biogenesis, ..
  32. Zhang Y, Si Y, Ma N. Meis1 promotes poly (rC)-binding protein 2 expression and inhibits angiotensin II-induced cardiomyocyte hypertrophy. IUBMB Life. 2016;68:13-22 pubmed publisher
    The poly(rC)-binding protein 2 (PCBP2) is currently reported to inhibit cardiac hypertrophy. However, how PCBP2 is regulated at transcriptional level remains unknown...
  33. Zell R, Ihle Y, Effenberger M, Seitz S, Wutzler P, Görlach M. Interaction of poly(rC)-binding protein 2 domains KH1 and KH3 with coxsackievirus RNA. Biochem Biophys Res Commun. 2008;377:500-503 pubmed publisher
    ..The data demonstrate interaction of isolated PCBP2 KH1 and KH3 domains to four distinct target sites within the 5'-nontranslated region of the CVB3 genomic RNA.
  34. Chase A, Daijogo S, Semler B. Inhibition of poliovirus-induced cleavage of cellular protein PCBP2 reduces the levels of viral RNA replication. J Virol. 2014;88:3192-201 pubmed publisher
    ..The host RNA-binding protein poly(rC) binding protein 2 (PCBP2) is involved in both processes in poliovirus infected cells...
  35. Xia P, Wang S, Xiong Z, Ye B, Huang L, Han Z, et al. IRTKS negatively regulates antiviral immunity through PCBP2 sumoylation-mediated MAVS degradation. Nat Commun. 2015;6:8132 pubmed publisher
    ..IRTKS recruits the E2 ligase Ubc9 to sumoylate PCBP2 in the nucleus, which causes its cytoplasmic translocation during viral infection...
  36. Boschi N, Takeuchi K, Sterling C, Tank A. Differential expression of polycytosine-binding protein isoforms in adrenal gland, locus coeruleus and midbrain. Neuroscience. 2015;286:1-12 pubmed publisher
    ..Our results suggest that: (1) PCBP2 is the predominant isoform in TH-positive cells of the rat midbrain; (2) PCBP3 is the predominant isoform in TH-..
  37. Liu H, Chen Z, Jin W, Barve A, Wan Y, Cheng K. Silencing of ?-complex protein-2 reverses alcohol- and cytokine-induced fibrogenesis in hepatic stellate cells. Liver Res. 2017;1:70-79 pubmed publisher
    complex protein-2 (?CP2) encoded by the poly (rC) binding protein 2(PCBP2) gene is responsible for the accumulation of type I collagen in fibrotic livers...
  38. Wu Y, Lou Q, Ge F, Xiong Q. Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos. Sci Rep. 2017;7:4022 pubmed publisher
    ..The results showed that genes encoding tropomyosin 1 (tpm1) and poly(rC) binding protein 2 (pcbp2) are direct miR-21 targets...
  39. Chen Z, Zhang W, Ni L, Wang G, Cao Y, Wu W, et al. Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury. Cell Mol Neurobiol. 2016;36:725-35 pubmed publisher
    ..It was reported that PCBP2 contributed to gastric cancer proliferation and survival through miR-34a, and knockdown of PCBP2 inhibited glioma ..
  40. van Dijk M, Visser A, Buabeng K, Poutsma A, van der Schors R, Oudejans C. Mutations within the LINC-HELLP non-coding RNA differentially bind ribosomal and RNA splicing complexes and negatively affect trophoblast differentiation. Hum Mol Genet. 2015;24:5475-85 pubmed publisher
    ..e. RNA splicing and the ribosome. YBX1, PCBP1, PCBP2, RPS6 and RPL7 were validated, and binding to these proteins was influenced by the HELLP mutations carried...
  41. Saul M, Stein S, Grez M, Jakobsson P, Steinhilber D, Suess B. UPF1 regulates myeloid cell functions and S100A9 expression by the hnRNP E2/miRNA-328 balance. Sci Rep. 2016;6:31995 pubmed publisher
    ..that UPF1 regulates the expression of genes with functions in inflammation and myeloid cell differentiation via hnRNP E2. The majority of the UPF1-regulated genes identified in monocytic cells contain a binding site for hnRNP E2 ..
  42. Ren C, Zhang J, Yan W, Zhang Y, Chen X. RNA-binding Protein PCBP2 Regulates p73 Expression and p73-dependent Antioxidant Defense. J Biol Chem. 2016;291:9629-37 pubmed publisher
    ..Here, we showed that TAp73 activity is regulated by RNA-binding protein PCBP2. Specifically, we found that knockdown or knock-out of PCBP2 reduces, whereas ectopic expression of PCBP2 ..
  43. Ji X, Wan J, Vishnu M, Xing Y, Liebhaber S. ?CP Poly(C) binding proteins act as global regulators of alternative polyadenylation. Mol Cell Biol. 2013;33:2560-73 pubmed publisher
    ..These findings predict a role for ?CPs in posttranscriptional control pathways that can alter the coding potential and/or levels of expression of subsets of mRNAs in the mammalian transcriptome. ..
  44. Tommerup N, Leffers H. Assignment of human KH-box-containing genes by in situ hybridization: HNRNPK maps to 9q21.32-q21.33, PCBP1 to 2p12-p13, and PCBP2 to 12q13.12-q13.13, distal to FRA12A. Genomics. 1996;32:297-8 pubmed
  45. Chkheidze A, Liebhaber S. A novel set of nuclear localization signals determine distributions of the alphaCP RNA-binding proteins. Mol Cell Biol. 2003;23:8405-15 pubmed
    ..This complexity of alphaCP distribution is likely to contribute to the diverse functions mediated by this group of abundant RNA-binding proteins. ..
  46. Asnani M, Pestova T, Hellen C. Initiation on the divergent Type I cadicivirus IRES: factor requirements and interactions with the translation apparatus. Nucleic Acids Res. 2016;44:3390-407 pubmed publisher
    ..requires eukaryotic initiation factors (eIFs) 1, 1A, 2, 3, 4A, 4B and 4G, and the poly(C) binding protein 2 (PCBP2), and starts with specific binding of eIF4G/eIF4A to d11...
  47. Ye J, Zhou G, Zhang Z, Sun L, He X, Zhou J. Poly (C)-binding protein 2 (PCBP2) promotes the progression of esophageal squamous cell carcinoma (ESCC) through regulating cellular proliferation and apoptosis. Pathol Res Pract. 2016;212:717-25 pubmed publisher
    b>PCBP2 (Poly(C)-binding protein 2) is a member of PCBP family, which has many functions including mRNA stabilization, translational silence and translational enhancement performed by their poly(C)-binding ability...
  48. Chang C, Luh H, Wang S, Lin H, Lee S, Hu S. The heterogeneous nuclear ribonucleoprotein K (hnRNP K) interacts with dengue virus core protein. DNA Cell Biol. 2001;20:569-77 pubmed
    ..Our results suggest that, on DEN virus infection, the multiple functions of cellular hnRNP K may be affected by the virus core protein. ..
  49. Ryu M, Zhang D, Protchenko O, Shakoury Elizeh M, Philpott C. PCBP1 and NCOA4 regulate erythroid iron storage and heme biosynthesis. J Clin Invest. 2017;127:1786-1797 pubmed publisher
    ..In cultured animal cells, iron chaperones poly rC-binding protein 1 (PCBP1) and PCBP2 deliver iron to ferritin, the sole cytosolic iron storage protein, and nuclear receptor coactivator 4 (NCOA4) ..
  50. Weidensdorfer D, Stöhr N, Baude A, Lederer M, Köhn M, Schierhorn A, et al. Control of c-myc mRNA stability by IGF2BP1-associated cytoplasmic RNPs. RNA. 2009;15:104-15 pubmed publisher
    ..Complex formation at the CRD presumably limits the transfer of c-myc mRNA to the polysomal fraction and subsequent translation-coupled decay. ..
  51. Ghanem L, Kromer A, Silverman I, Chatterji P, Traxler E, Penzo Méndez A, et al. The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development. Mol Cell Biol. 2016;36:304-19 pubmed publisher
    ..The two major Pcbp isoforms, Pcbp2 and Pcbp1, are robustly expressed in a wide range of tissues and exert both nuclear and cytoplasmic controls over ..
  52. Xin Z, Han W, Zhao Z, Xia Q, Yin B, Yuan J, et al. PCBP2 enhances the antiviral activity of IFN-? against HCV by stabilizing the mRNA of STAT1 and STAT2. PLoS ONE. 2011;6:e25419 pubmed publisher
    ..We found that the protein PCBP2 is down-regulated in HCV-replicon containing cells (R1b)...
  53. Gelfand B, Wright C, Kim Y, Yasuma T, Yasuma R, Li S, et al. Iron Toxicity in the Retina Requires Alu RNA and the NLRP3 Inflammasome. Cell Rep. 2015;11:1686-93 pubmed publisher
    ..RNA accumulation is due to suppression of DICER1 via sequestration of the co-factor poly(C)-binding protein 2 (PCBP2)...
  54. Mao X, Liu J, Chen C, Zhang W, Qian R, Chen X, et al. PCBP2 Modulates Neural Apoptosis and Astrocyte Proliferation After Spinal Cord Injury. Neurochem Res. 2016;41:2401-14 pubmed publisher
    b>PCBP2, a member of the poly(C)-binding protein (PCBP) family, plays a pivotal role in posttranscriptional and translational regulation by interacting with single-stranded poly(C) motifs in target mRNAs...
  55. Navratilova Z, Novosadova E, Hagemann Jensen M, Kullberg S, Kolek V, Grunewald J, et al. Expression Profile of Six RNA-Binding Proteins in Pulmonary Sarcoidosis. PLoS ONE. 2016;11:e0161669 pubmed publisher
    ..We investigated in vivo expression of six RBPs (AUF1, HuR, NCL, TIA, TIAR, PCBP2) and two inhibitors of proteolytic enzymes (RECK, PTEN) in pulmonary sarcoidosis and compared it to the expression ..
  56. Chen X, Hao B, Liu Y, Dai D, Han G, Li Y, et al. The histone deacetylase SIRT6 suppresses the expression of the RNA-binding protein PCBP2 in glioma. Biochem Biophys Res Commun. 2014;446:364-9 pubmed publisher
    ..Previous evidence and our present data show that PCBP2 is over-expressed in human glioma tissues and predicts poor outcome...
  57. Qin Y, Xue B, Liu C, Wang X, Tian R, Xie Q, et al. NLRX1 Mediates MAVS Degradation To Attenuate the Hepatitis C Virus-Induced Innate Immune Response through PCBP2. J Virol. 2017;91: pubmed publisher
    ..Moreover, poly(rC) binding protein 2 (PCBP2) interacts with NLRX1 to participate in the NLRX1-induced degradation of MAVS and the inhibition of antiviral ..
  58. Zhang X, Hua L, Yan D, Zhao F, Liu J, Zhou H, et al. Overexpression of PCBP2 contributes to poor prognosis and enhanced cell growth in human hepatocellular carcinoma. Oncol Rep. 2016;36:3456-3464 pubmed publisher
    Poly(C)?binding protein 2 (PCBP2) is a member of the PCBP family, and plays an important role in post?transcriptional and translational regulation of various signaling molecules through direct binding to single?stranded poly(C) motifs...
  59. Li F, Bullough K, Vashisht A, Wohlschlegel J, Philpott C. Poly(rC)-Binding Protein 2 Regulates Hippo Signaling To Control Growth in Breast Epithelial Cells. Mol Cell Biol. 2016;36:2121-31 pubmed publisher
    ..Here, we show that PCBP2 forms a complex with the Hippo pathway components Salvador (Sav1), Mst1, Mst2, and Lats1 in human cells and mouse ..
  60. Xia N, Tang Z, Wang C, Xu G, Nie X, Zhang W, et al. PCBP2 regulates hepatic insulin sensitivity via HIF-1α and STAT3 pathway in HepG2 cells. Biochem Biophys Res Commun. 2015;463:116-22 pubmed publisher
    ..However, there are little studies about the roles of PCBPs in insulin resistance. PCBP2 is the member of the RNA-binding proteins and is thought to participate in regulating hypoxia inducible factor-1 (..
  61. Zhao Z, Li Y, Jain A, Chen Z, Liu H, Jin W, et al. Development of a peptide-modified siRNA nanocomplex for hepatic stellate cells. Nanomedicine. 2018;14:51-61 pubmed publisher
    ..cholesterol, and vitamin A as the targeting ligands for the neutravidin-based siRNA nanocomplex to deliver PCBP2 siRNA, a potentially antifibrotic agent, to HSCs...
  62. Li Y, Lin L, Li Z, Ye X, Xiong K, Aryal B, et al. Iron homeostasis regulates the activity of the microRNA pathway through poly(C)-binding protein 2. Cell Metab. 2012;15:895-904 pubmed publisher
    ..We show that cytosolic iron could regulate the activity of the miRNA pathway through poly(C)-binding protein 2 (PCBP2). PCBP2 is associated with Dicer and promotes the processing of miRNA precursors...
  63. Evans J, Mitchell S, Spriggs K, Ostrowski J, Bomsztyk K, Ostarek D, et al. Members of the poly (rC) binding protein family stimulate the activity of the c-myc internal ribosome entry segment in vitro and in vivo. Oncogene. 2003;22:8012-20 pubmed
    ..members of the poly (rC) binding protein family, poly (rC) binding protein 1 (PCBP1), poly (rC) binding protein 2 (PCBP2) and hnRNPK were able to activate the IRES in vitro up to threefold when added in combination with upstream of N-..
  64. Pickering B, Mitchell S, Evans J, Willis A. Polypyrimidine tract binding protein and poly r(C) binding protein 1 interact with the BAG-1 IRES and stimulate its activity in vitro and in vivo. Nucleic Acids Res. 2003;31:639-46 pubmed
    ..The minimum section of the RNA that still retained activity was determined and both PCBP1 and PTB interacted with this region suggesting that these proteins are essential for Bag-1 IRES function. ..
  65. Jang S, Shin H, Lee J, Kim Y, Bak G, Lee Y. Regulation of BC200 RNA-mediated translation inhibition by hnRNP E1 and E2. FEBS Lett. 2017;591:393-405 pubmed publisher
    ..Here, we performed yeast three-hybrid screening and identified hnRNP E1 and hnRNP E2 as BC200 RNA-interacting proteins...
  66. Yanatori I, Richardson D, Toyokuni S, Kishi F. The iron chaperone poly(rC)-binding protein 2 forms a metabolon with the heme oxygenase 1/cytochrome P450 reductase complex for heme catabolism and iron transfer. J Biol Chem. 2017;292:13205-13229 pubmed publisher
    ..Recently, we demonstrated that the iron chaperone poly(rC)-binding protein 2 (PCBP2) can directly receive ferrous iron from DMT1 or transfer iron to the iron exporter, ferroportin 1...
  67. Kiledjian M, DeMaria C, Brewer G, Novick K. Identification of AUF1 (heterogeneous nuclear ribonucleoprotein D) as a component of the alpha-globin mRNA stability complex. Mol Cell Biol. 1997;17:4870-6 pubmed
    ..AUF1/hnRNP-D could, therefore, be a general mRNA turnover factor involved in both stabilization and decay of mRNA. ..
  68. Ji X, Park J, Bahrami Samani E, Lin L, Duncan Lewis C, Pherribo G, et al. αCP binding to a cytosine-rich subset of polypyrimidine tracts drives a novel pathway of cassette exon splicing in the mammalian transcriptome. Nucleic Acids Res. 2016;44:2283-97 pubmed publisher
  69. Leidgens S, Bullough K, Shi H, Li F, Shakoury Elizeh M, Yabe T, et al. Each member of the poly-r(C)-binding protein 1 (PCBP) family exhibits iron chaperone activity toward ferritin. J Biol Chem. 2013;288:17791-802 pubmed publisher
    ..Poly r(C)-binding protein 1 (PCBP1) is an iron chaperone for ferritin; both PCBP1 and its paralog PCBP2 are required for iron delivery to the prolyl hydroxylase that regulates HIF1...
  70. Zell R, Ihle Y, Seitz S, Gündel U, Wutzler P, Görlach M. Poly(rC)-binding protein 2 interacts with the oligo(rC) tract of coxsackievirus B3. Biochem Biophys Res Commun. 2008;366:917-21 pubmed
    The human poly(rC)-binding protein (PCBP) 2 is known to interact with enteroviral RNA. Here, the interaction of PCBP2 with RNA target sequences at the 5' end of the coxsackievirus B3 genome was investigated...
  71. Koganti S, Clark C, Zhi J, Li X, Chen E, Chakrabortty S, et al. Cellular STAT3 functions via PCBP2 to restrain Epstein-Barr Virus lytic activation in B lymphocytes. J Virol. 2015;89:5002-11 pubmed publisher
    ..and a publically available STAT3 chromatin immunoprecipitation sequencing (ChIP-Seq) data set to identify cellular PCBP2 [poly(C)-binding protein 2], an RNA-binding protein, as a transcriptional target of STAT3 in refractory cells...
  72. Frey A, Nandal A, Park J, Smith P, Yabe T, Ryu M, et al. Iron chaperones PCBP1 and PCBP2 mediate the metallation of the dinuclear iron enzyme deoxyhypusine hydroxylase. Proc Natl Acad Sci U S A. 2014;111:8031-6 pubmed publisher
    ..Poly(rC)-binding proteins PCBP1 and PCBP2 are multifunctional adaptor proteins that bind iron and deliver it to ferritin for storage or to prolyl and ..
  73. Wan C, Gong C, Zhang H, Hua L, Li X, Chen X, et al. β2-adrenergic receptor signaling promotes pancreatic ductal adenocarcinoma (PDAC) progression through facilitating PCBP2-dependent c-myc expression. Cancer Lett. 2016;373:67-76 pubmed publisher
    ..In this report, we identified poly(rC)-binding protein 2 (PCBP2) as a novel binding partner for β2-AR using immunoprecipitation-mass spectrometry (IP-MS) approach...
  74. Funke B, Zuleger B, Benavente R, Schuster T, Goller M, Stevenin J, et al. The mouse poly(C)-binding protein exists in multiple isoforms and interacts with several RNA-binding proteins. Nucleic Acids Res. 1996;24:3821-8 pubmed
    ..The interaction between mCBP and splicing factor 9G8 was confirmed in vivo. These results suggest a function of mCBP in RNA metabolism. ..
  75. Bedard K, Daijogo S, Semler B. A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiation. EMBO J. 2007;26:459-67 pubmed
    ..We found that SRp20 interacts with the cellular RNA-binding protein, PCBP2, a protein that binds to IRES sequences within the genomic RNAs of certain picornaviruses and is required for ..
  76. Kim J, Hahm B, Kim Y, Choi M, Jang S. Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm. J Mol Biol. 2000;298:395-405 pubmed
    ..Among them, hnRNP A, D, E, I, and K are known to shuttle between the nucleus and the cytoplasm. hnRNP E2 has been seen to stabilize alpha-globin mRNA and to enhance polioviral mRNA translation...
  77. Hadian K, Vincendeau M, Mäusbacher N, Nagel D, Hauck S, Ueffing M, et al. Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in the HIV Rev protein. J Biol Chem. 2009;284:33384-91 pubmed publisher
  78. Makeyev A, Liebhaber S. Identification of two novel mammalian genes establishes a subfamily of KH-domain RNA-binding proteins. Genomics. 2000;67:301-16 pubmed
    ..These data establish a new subfamily of genes predicted to encode closely related KH-containing RNA-binding proteins with potential functions in posttranscriptional controls. ..
  79. Hu C, Liu Y, Zhang H, Huang G. The RNA-binding protein PCBP2 facilitates gastric carcinoma growth by targeting miR-34a. Biochem Biophys Res Commun. 2014;448:437-42 pubmed publisher
    ..Here in the present study, we identify the RNA-binding protein PCBP2 as an oncogenic protein in human gastric carcinoma...
  80. Palusa S, Ndaluka C, Bowen R, Wilusz C, Wilusz J. The 3' untranslated region of the rabies virus glycoprotein mRNA specifically interacts with cellular PCBP2 protein and promotes transcript stability. PLoS ONE. 2012;7:e33561 pubmed publisher
    ..the rabies virus glycoprotein mRNA also selectively interacts with the cellular poly(rC)-binding protein 2 (PCBP2), a factor known to influence mRNA stability...
  81. Davidson Y, Robinson A, Flood L, Rollinson S, Benson B, Asi Y, et al. Heterogeneous ribonuclear protein E2 (hnRNP E2) is associated with TDP-43-immunoreactive neurites in Semantic Dementia but not with other TDP-43 pathological subtypes of Frontotemporal Lobar Degeneration. Acta Neuropathol Commun. 2017;5:54 pubmed publisher
    ..Immunostaining for hnRNP E2 was performed on sections of frontal and temporal cortex with hippocampus from 80 patients with FTLD, stratified ..
  82. Leffers H, Dejgaard K, Celis J. Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains. Eur J Biochem. 1995;230:447-53 pubmed
    ..Escherichia coli expressed proteins also bound poly(rG), albeit at a lower level. In addition, PCBP-2 bound poly(rU), whereas very little binding to poly(rA) was observed for both proteins. ..
  83. Luo K, Zhuang K. High expression of PCBP2 is associated with progression and poor prognosis in patients with glioblastoma. Biomed Pharmacother. 2017;94:659-665 pubmed publisher
    Poly(C)-binding protein 2 (PCBP2) has been found to have ambiguous functions in a variety of cancers. However, the specific biological function of PCBP2 and its mechanism in glioblastoma remain unclear...
  84. Wang Y, Wang J, Gao L, Stamm S, Andreadis A. An SRp75/hnRNPG complex interacting with hnRNPE2 regulates the 5' splice site of tau exon 10, whose misregulation causes frontotemporal dementia. Gene. 2011;485:130-8 pubmed publisher
    ..of exon 10 splicing: serine/arginine-rich protein SRp75 and heterogeneous nuclear ribonucleoproteins hnRNPG and hnRNPE2. SRp75 and hnRNPG inhibit splicing of exon 10 whereas hnRNPE2 activates it...
  85. Abraham R, Geyer S, Price Troska T, Allmer C, Kyle R, Gertz M, et al. Immunoglobulin light chain variable (V) region genes influence clinical presentation and outcome in light chain-associated amyloidosis (AL). Blood. 2003;101:3801-8 pubmed
    ..The presence of renal disease, on the other hand, was associated with improved survival. Therefore, identification of the clonal VL gene in AL has important implications in determining clinical outcome. ..
  86. Tang S, Gao Y, Chen X. MicroRNA-214 targets PCBP2 to suppress the proliferation and growth of glioma cells. Int J Clin Exp Pathol. 2015;8:12571-6 pubmed
    b>PCBP2, a member of the poly(C)-binding protein (PCBP) family, is involved in posttranscriptional and translational regulation by interacting with single-stranded poly(C) motifs in target mRNAs...
  87. Shimizu Y, Mullins N, Blanchard Z, ElShamy W. BRCA1/p220 loss triggers BRCA1-IRIS overexpression via mRNA stabilization in breast cancer cells. Oncotarget. 2012;3:299-313 pubmed
    ..normal human mammary epithelial (HME) cells reduces expression of two RNA-destabilizing proteins, namely AUF1 and pCBP2, both proteins bind and destabilize BRCA1-IRIS mRNA...
  88. Chase A, Semler B. Differential cleavage of IRES trans-acting factors (ITAFs) in cells infected by human rhinovirus. Virology. 2014;449:35-44 pubmed publisher
    ..Host proteins poly(rC) binding protein 2 (PCBP2) and polypyrimidine tract binding protein (PTB) are cleaved by a viral proteinase during the course of infection ..
  89. Molinaro R, Jha B, Malathi K, Varambally S, Chinnaiyan A, Silverman R. Selection and cloning of poly(rC)-binding protein 2 and Raf kinase inhibitor protein RNA activators of 2',5'-oligoadenylate synthetase from prostate cancer cells. Nucleic Acids Res. 2006;34:6684-95 pubmed
    ..We thus identified mRNAs for Raf kinase inhibitor protein (RKIP) and poly(rC)-binding protein 2 (PCBP2) that bind and potently activate OAS...
  90. Murahashi M, Simizu S, Morioka M, Umezawa K. Identification of poly(rC) binding protein 2 (PCBP2) as a target protein of immunosuppressive agent 15-deoxyspergualin. Biochem Biophys Res Commun. 2016;476:445-449 pubmed publisher
    ..In the present research, we identified poly(rC) binding protein 2 (PCBP2) as a DSG-binding protein using this probe. DSG was confirmed to bind to PCBP2 by pull-down assay...
  91. Leung J, Fishbane N, Jones M, Morin A, Xu S, Liu J, et al. Longitudinal study of surrogate aging measures during human immunodeficiency virus seroconversion. Aging (Albany NY). 2017;9:687-705 pubmed publisher
    ..035). CpG sites corresponding to PCBP2 and CSRNP1 were differentially methylated between T1 and T2 at a q-value <0.05...