Gene Symbol: PASK
Description: PAS domain containing serine/threonine kinase
Alias: PASKIN, STK37, PAS domain-containing serine/threonine-protein kinase, per-arnt-sim (PAS) domain kinase
Species: human
Products:     PASK

Top Publications

  1. Rutter J, Michnoff C, Harper S, Gardner K, McKnight S. PAS kinase: an evolutionarily conserved PAS domain-regulated serine/threonine kinase. Proc Natl Acad Sci U S A. 2001;98:8991-6 pubmed
    ..Similarly regulated kinases are rare in eukaryotes and are to date completely absent in mammals. PAS kinase (PASK) is an evolutionarily conserved gene product present in yeast, flies, and mammals...
  2. Amezcua C, Harper S, Rutter J, Gardner K. Structure and interactions of PAS kinase N-terminal PAS domain: model for intramolecular kinase regulation. Structure. 2002;10:1349-61 pubmed
    ..Structural and functional analyses of point mutants demonstrate that the compound and ligand binding regions are linked, suggesting that the PAS domain serves as a ligand-regulated switch for this eukaryotic signaling system. ..
  3. MacDonald P, Rorsman P. Per-arnt-sim (PAS) domain kinase (PASK) as a regulator of glucagon secretion. Diabetologia. 2011;54:719-21 pubmed publisher
    ..provides intriguing new insight into a metabolic sensing pathway mediated by the per-arnt-sim (PAS) domain kinase (PASK) that may contribute to both the paracrine and the intrinsic glucose regulation of alpha cells...
  4. Da Silva Xavier G, Rutter J, Rutter G. Involvement of Per-Arnt-Sim (PAS) kinase in the stimulation of preproinsulin and pancreatic duodenum homeobox 1 gene expression by glucose. Proc Natl Acad Sci U S A. 2004;101:8319-24 pubmed
    ..Although the PAS domain of the mammalian PAS kinase (PASK) is closely related to the bacterial oxygen sensor FixL, it is unclear whether PASK activity is changed in ..
  5. Wilson W, Skurat A, Probst B, de Paoli Roach A, Roach P, Rutter J. Control of mammalian glycogen synthase by PAS kinase. Proc Natl Acad Sci U S A. 2005;102:16596-601 pubmed
    ..This mode of regulation provides a mechanism for metabolic status to impinge directly on the cellular decision of whether to store or use available energy. ..
  6. Eckhardt K, Troger J, Reissmann J, Katschinski D, Wagner K, Stengel P, et al. Male germ cell expression of the PAS domain kinase PASKIN and its novel target eukaryotic translation elongation factor eEF1A1. Cell Physiol Biochem. 2007;20:227-40 pubmed
    b>PASKIN links energy flux and protein synthesis in yeast, regulates glycogen synthesis in mammals, and has been implicated in glucose-stimulated insulin production in pancreatic beta-cells...
  7. Kikani C, Antonysamy S, Bonanno J, Romero R, Zhang F, Russell M, et al. Structural bases of PAS domain-regulated kinase (PASK) activation in the absence of activation loop phosphorylation. J Biol Chem. 2010;285:41034-43 pubmed publisher
    Per-Arnt-Sim (PAS) domain-containing protein kinase (PASK) is an evolutionary conserved protein kinase that coordinates cellular metabolism with metabolic demand in yeast and mammals...
  8. Schl fli P, Tr ger J, Eckhardt K, Borter E, Spielmann P, Wenger R. Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN. FEBS J. 2011;278:1757-68 pubmed publisher
    The Per-Arnt-Sim (PAS) domain serine/threonine kinase PASKIN, or PAS kinase, links energy flux and protein synthesis in yeast, regulates glycogen synthesis and protein translation in mammals, and might be involved in insulin regulation in ..
  9. Hofer T, Spielmann P, Stengel P, Stier B, Katschinski D, Desbaillets I, et al. Mammalian PASKIN, a PAS-serine/threonine kinase related to bacterial oxygen sensors. Biochem Biophys Res Commun. 2001;288:757-64 pubmed
    ..We identified a novel mammalian PAS protein (PASKIN) containing a domain architecture resembling FixL...

More Information


  1. da Silva Xavier G, Farhan H, Kim H, Caxaria S, Johnson P, Hughes S, et al. Per-arnt-sim (PAS) domain-containing protein kinase is downregulated in human islets in type 2 diabetes and regulates glucagon secretion. Diabetologia. 2011;54:819-27 pubmed publisher
    We assessed whether per-arnt-sim (PAS) domain-containing protein kinase (PASK) is involved in the regulation of glucagon secretion...
  2. Pape J, Newey C, Burrell H, Workman A, Perry K, Bikman B, et al. Per-Arnt-Sim Kinase (PASK) Deficiency Increases Cellular Respiration on a Standard Diet and Decreases Liver Triglyceride Accumulation on a Western High-Fat High-Sugar Diet. Nutrients. 2018;10: pubmed publisher
    ..the first characterization of the effects of western diet (high-fat high-sugar, HFHS) on Per-Arnt-Sim kinase mice (PASK-/-) and the first characterization of both male and female PASK-/- mice...
  3. Dongil P, Perez Garcia A, Hurtado Carneiro V, Herrero de Dios C, Blazquez E, Alvarez E, et al. Pas Kinase Deficiency Triggers Antioxidant Mechanisms in the Liver. Sci Rep. 2018;8:13810 pubmed publisher
    ..b>PASK (PAS Domain Kinase) is a nutrient and bioenergetic sensor...
  4. Song K, Zhang M, Hu J, Liu Y, Liu Y, Wang Y, et al. Methane-rich saline attenuates ischemia/reperfusion injury of abdominal skin flaps in rats via regulating apoptosis level. BMC Surg. 2015;15:92 pubmed publisher
    ..Moreover, pASK-1, pJNK, Bcl-2 and Bax were detected by immunohistochemical technology...
  5. Zhang D, Zhang J, Wang Y, Liu Y, Liu G, Li X. Per-Arnt-Sim Kinase (PASK): An Emerging Regulator of Mammalian Glucose and Lipid Metabolism. Nutrients. 2015;7:7437-50 pubmed publisher
    Per-Arnt-Sim Kinase (PASK) is an evolutionarily-conserved nutrient-responsive protein kinase that regulates lipid and glucose metabolism, mitochondrial respiration, phosphorylation, and gene expression...
  6. Huang F, Tang J, He L, Ding X, Huang S, Zhang Y, et al. Heterologous expression and antitumor activity analysis of syringolin from Pseudomonas syringae pv. syringae B728a. Microb Cell Fact. 2018;17:31 pubmed publisher
    ..A 22 kb genomic fragment containing the sylA-sylE gene cluster was cloned into the pASK vector, and the obtained recombinant plasmid was transferred into Streptomyces coelicolor and Streptomyces lividans ..
  7. Azevedo C, Saiardi A. The new world of inorganic polyphosphates. Biochem Soc Trans. 2016;44:13-7 pubmed publisher
    ..Polyphosphorylation occurs within a conserved N-terminal polyacidic serine (S) and lysine (K) rich (PASK) cluster...
  8. Semplici F, Mondragon A, Macintyre B, Madeyski Bengston K, Persson Kry A, Barr S, et al. Cell type-specific deletion in mice reveals roles for PAS kinase in insulin and glucagon production. Diabetologia. 2016;59:1938-47 pubmed publisher
    Per-Arnt-Sim kinase (PASK) is a nutrient-regulated domain-containing protein kinase previously implicated in the control of insulin gene expression and glucagon secretion...
  9. Solini A, Sebastiani G, Nigi L, Santini E, Rossi C, Dotta F. Dapagliflozin modulates glucagon secretion in an SGLT2-independent manner in murine alpha cells. Diabetes Metab. 2017;43:512-520 pubmed publisher
    ..Looking at the candidate intracellular signalling pathway, reduced PASK and increased AMPK-?2 expression were also detected...
  10. Kikani C, Wu X, Paul L, Sabic H, Shen Z, Shakya A, et al. Pask integrates hormonal signaling with histone modification via Wdr5 phosphorylation to drive myogenesis. elife. 2016;5: pubmed publisher
    PAS domain containing protein kinase (Pask) is an evolutionarily conserved protein kinase implicated in energy homeostasis and metabolic regulation across eukaryotic species...
  11. Boumaiza M, Carmona F, Poli M, Asperti M, Gianoncelli A, Bertuzzi M, et al. Production and characterization of functional recombinant hybrid heteropolymers of camel hepcidin and human ferritin H and L chains. Protein Eng Des Sel. 2017;30:77-84 pubmed publisher
    ..functional properties the cDNA coding for camel hepcidin-25 was cloned at the 5'end of human FTH sequence into the pASK-IBA43plus vector for expression in Escherichia coli The recombinant fusion hepcidin-ferritin-H subunit was isolated ..
  12. Pérez García A, Dongil P, Hurtado Carneiro V, Blazquez E, Sanz C, Alvarez E. High-fat diet alters PAS kinase regulation by fasting and feeding in liver. J Nutr Biochem. 2018;57:14-25 pubmed publisher
    ..Per-Arnt-Sim (PAS) kinase (PASK) controls glucose homeostasis and energy metabolism in response to nutritional status...
  13. Liu Y, Liu Y, Ma X, Xiao Y, Wang Y, Zhang M, et al. Hydrogen-rich saline attenuates skin ischemia/reperfusion induced apoptosis via regulating Bax/Bcl-2 ratio and ASK-1/JNK pathway. J Plast Reconstr Aesthet Surg. 2015;68:e147-56 pubmed publisher
    ..b>pASK-1, pJNK, Bcl-2 and Bax were examined by immunodetection. In addition Bcl-2, Bax and caspase-3 were detected by qPCR...
  14. Xiao Y, Liu Y, Li J, Ma X, Wang Y, Liu Y, et al. Hyperbaric oxygen preconditioning inhibits skin flap apoptosis in a rat ischemia-reperfusion model. J Surg Res. 2015;199:732-9 pubmed publisher
    ..Protein expression of phosphorylated apoptosis signal-regulating kinase 1 (pASK-1), phosphorylated c-Jun N-terminal kinase (pJNK), B-cell lymphoma-2 (Bcl-2), and Bcl2-associated X protein (Bax) ..
  15. Stetz M, Carter M, Wand A. Optimized expression and purification of biophysical quantities of Lac repressor and Lac repressor regulatory domain. Protein Expr Purif. 2016;123:75-82 pubmed publisher
    ..The expression vector is an adaptation of the pASK backbone wherein expression of the lacI gene is regulated by an anhydrotetracyline inducible tetA promoter and the ..
  16. Bai M, Liu Y, Yin D, Zhang M, Wang Y, Ma X, et al. Inhibition of c-Jun N-terminal kinase signaling suppresses skin flap apoptosis in a rat ischemia and/or reperfusion model. J Surg Res. 2016;206:337-346 pubmed publisher
    ..Early apoptosis was observed using TdT-mediated dUTP-X nick end-labeling staining. pASK-1, pJNK, Bcl-2, and Bax were examined by immunodetection...
  17. Azevedo C, Livermore T, Saiardi A. Protein polyphosphorylation of lysine residues by inorganic polyphosphate. Mol Cell. 2015;58:71-82 pubmed publisher
    ..This modification occurs on lysine (K) residues within a conserved N-terminal polyacidic serine (S) and K-rich (PASK) cluster...
  18. Perez Garcia A, Dongil P, Hurtado Carneiro V, Blazquez E, Sanz C, Alvarez E. PAS Kinase deficiency alters the glucokinase function and hepatic metabolism. Sci Rep. 2018;8:11091 pubmed publisher
    ..Our objective was to determine whether PAS kinase (PASK), a nutrient sensor, could be affecting the expression or activity of liver GCK and the response to fasting and ..
  19. Pina Pedrero S, Olvera A, Bensaid A. The extended leader peptide of Haemophilus parasuis trimeric autotransporters conditions their protein expression in Escherichia coli. Protein Expr Purif. 2017;133:15-24 pubmed publisher
    ..The 13 genes encoding the VtaA1 to VtaA13 passenger domains of the strain Nagasaki were cloned in the pASK-IBA33plus plasmid and expressed in E. coli...
  20. Ceulemans H, Van Eynde A, Pérez Callejón E, Beullens M, Stalmans W, Bollen M. Structure and splice products of the human gene encoding sds22, a putative mitotic regulator of protein phosphatase-1. Eur J Biochem. 1999;262:36-42 pubmed
    ..The 5'-flanking region of PPP1R7 contains two NF-Y-binding CCAAT boxes near the transcription start site and potential binding sites for the transcription factors c-Myb, Ik-2 and NF-1, which are conserved in the mouse gene. ..
  21. Ke Y, Ng C, Hunter M, Mann S, Heide J, Hill A, et al. Trafficking defects in PAS domain mutant Kv11.1 channels: roles of reduced domain stability and altered domain-domain interactions. Biochem J. 2013;454:69-77 pubmed publisher
  22. Hao Y, Liu X, Lu X, Yang X, Wang L, Chen S, et al. Genome-wide association study in Han Chinese identifies three novel loci for human height. Hum Genet. 2013;132:681-9 pubmed publisher
    ..80 × 10(-10)). In addition, we provided evidence supporting 35 SNPs identified by previous GWAS (P < 0.05). Our study provides new insights into the genetic determination of biological regulation of human height. ..
  23. Semplici F, Vaxillaire M, Fogarty S, Semache M, Bonnefond A, Fontes G, et al. Human mutation within Per-Arnt-Sim (PAS) domain-containing protein kinase (PASK) causes basal insulin hypersecretion. J Biol Chem. 2011;286:44005-14 pubmed publisher
    PAS kinase (PASK) is a glucose-regulated protein kinase involved in the control of pancreatic islet hormone release and insulin sensitivity...
  24. Semache M, Zarrouki B, Fontes G, Fogarty S, Kikani C, Chawki M, et al. Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3? in pancreatic ?-cells. J Biol Chem. 2013;288:24825-33 pubmed publisher
    ..We previously showed that the serine/threonine Per-Arnt-Sim domain-containing kinase (PASK) regulates insulin gene transcription via PDX-1. However, the mechanisms underlying this regulation are unknown...
  25. Grose J, Rutter J. The role of PAS kinase in PASsing the glucose signal. Sensors (Basel). 2010;10:5668-82 pubmed publisher
    ..Although bona fide PAS kinase substrates are scarce, in vitro substrate searches provide putative targets for exploration. ..