pancreatic ribonuclease

Summary

Gene Symbol: pancreatic ribonuclease
Description: ribonuclease, RNase A family, 1 (pancreatic)
Alias: RIB1, RNS1, HP-RNase, RIB-1, RNase 1, RNase A, RNase upI-1, ribonuclease 1, ribonuclease A, ribonuclease pancreatic
Species: human

Top Publications

  1. ncbi Degradation of double-stranded RNA by human pancreatic ribonuclease: crucial role of noncatalytic basic amino acid residues
    Salvatore Sorrentino
    Department of Biological Chemistry, University Federico II of Naples, Naples, Italy
    Biochemistry 42:10182-90. 2003
  2. ncbi Interaction of human pancreatic ribonuclease with human ribonuclease inhibitor. Generation of inhibitor-resistant cytotoxic variants
    D Gaur
    Immunochemistry Laboratory, National Institute of Immunology, Aruna Asaf Ali Road, New Delhi 110067, India
    J Biol Chem 276:24978-84. 2001
  3. ncbi Glycine 38 is crucial for the ribonucleolytic activity of human pancreatic ribonuclease on double-stranded RNA
    Deepak Gaur
    Immunochemistry Laboratory, National Institute of Immunology, New Delhi 110 067, India
    Biochem Biophys Res Commun 297:390-5. 2002
  4. ncbi Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the design of cytotoxic ribonucleases
    J Pous
    Institut de Biologia Molecular de Barcelona, CID CSIC, Jordi Girona 18 26, Barcelona, 08034, Spain
    J Mol Biol 303:49-60. 2000
  5. ncbi Glycosylation of human pancreatic ribonuclease: differences between normal and tumor states
    Rosa Peracaula
    Departament de Biologia, Universitat de Girona, Campus de Montilivi s n 17071, Girona, Spain
    Glycobiology 13:227-44. 2003
  6. ncbi Three-dimensional structure of human RNase 1 delta N7 at 1.9 A resolution
    J Pous
    Institut de Biologia Molecular de Barcelona, CID CSIC, Jordi Girona 18 26, 08034 Barcelona, Spain
    Acta Crystallogr D Biol Crystallogr 57:498-505. 2001
  7. ncbi The amino acid sequence of human pancreatic ribonuclease
    J J Beintema
    Anal Biochem 136:48-64. 1984
  8. ncbi Human endothelial cells selectively express large amounts of pancreatic-type ribonuclease (RNase 1)
    Julien B P Landré
    INSERM EPI 0113, Universite Bordeaux I, Avenue des Facultes, Talence, France
    J Cell Biochem 86:540-52. 2002
  9. ncbi Killing of endothelial cells and release of arachidonic acid. Synergistic effects among hydrogen peroxide, membrane-damaging agents, cationic substances, and proteinases and their modulation by inhibitors
    I Ginsburg
    Department of Oral Biology, Hebrew University Hadassah School of Medicine, Jerusalem, Israel
    Inflammation 17:295-319. 1993
  10. pmc Molecular evolution of B6 enzymes: binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzyme
    Rosa A Vacca
    Institute of Biomembranes and Bioenergetics, CNR, Via Amendola 165 A, I 70126 Bari, Italy
    BMC Biochem 9:17. 2008

Research Grants

  1. FLAVOPROTEINS IN FATTY ACID METABOLISM
    Colin Thorpe; Fiscal Year: 2003
  2. RIBONUCLEASES IN CANCER CHEMOTHERAPY
    RONALD RAINES; Fiscal Year: 2007
  3. CHEMISTRY AND BIOLOGY OF RIBONUCLEASE A
    RONALD RAINES; Fiscal Year: 2004
  4. RIBONUCLEASES IN CANCER CHEMOTHERAPY
    Ronald T Raines; Fiscal Year: 2010
  5. Evolution of vertebrate sensory genes
    Jianzhi Zhang; Fiscal Year: 2010
  6. Functional genomic approaches to duplicate gene evolution
    Jianzhi Zhang; Fiscal Year: 2010
  7. Functional genomic approaches to duplicate gene evolution
    Jianzhi Zhang; Fiscal Year: 2010
  8. Evolution of vertebrate sensory genes
    Jianzhi Zhang; Fiscal Year: 2009
  9. RNases for understanding the origin of new gene function
    Jianzhi Zhang; Fiscal Year: 2006
  10. RNases for understanding the origin of new gene function
    Jianzhi Zhang; Fiscal Year: 2007

Scientific Experts

Detail Information

Publications164 found, 100 shown here

  1. ncbi Degradation of double-stranded RNA by human pancreatic ribonuclease: crucial role of noncatalytic basic amino acid residues
    Salvatore Sorrentino
    Department of Biological Chemistry, University Federico II of Naples, Naples, Italy
    Biochemistry 42:10182-90. 2003
    ..Human pancreatic ribonuclease (HP-RNase) is the most powerful enzyme to degrade dsRNA within the tetrapod RNase superfamily, being 500-..
  2. ncbi Interaction of human pancreatic ribonuclease with human ribonuclease inhibitor. Generation of inhibitor-resistant cytotoxic variants
    D Gaur
    Immunochemistry Laboratory, National Institute of Immunology, Aruna Asaf Ali Road, New Delhi 110067, India
    J Biol Chem 276:24978-84. 2001
    ..Human pancreatic ribonuclease (HPR) is structurally and functionally very similar to bovine RNase A and interacts with human RI with a ..
  3. ncbi Glycine 38 is crucial for the ribonucleolytic activity of human pancreatic ribonuclease on double-stranded RNA
    Deepak Gaur
    Immunochemistry Laboratory, National Institute of Immunology, New Delhi 110 067, India
    Biochem Biophys Res Commun 297:390-5. 2002
    Human pancreatic ribonuclease (HPR) and bovine seminal ribonuclease (BS-RNase) exhibit significantly higher activity against double stranded RNA (dsRNA), compared to RNase A...
  4. ncbi Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the design of cytotoxic ribonucleases
    J Pous
    Institut de Biologia Molecular de Barcelona, CID CSIC, Jordi Girona 18 26, Barcelona, 08034, Spain
    J Mol Biol 303:49-60. 2000
    We have determined the crystal structure of a human pancreatic ribonuclease or RNase 1 variant at 1.65 A resolution. Five residues in the N-terminal region were substituted by the corresponding amino acids of the bovine seminal RNase...
  5. ncbi Glycosylation of human pancreatic ribonuclease: differences between normal and tumor states
    Rosa Peracaula
    Departament de Biologia, Universitat de Girona, Campus de Montilivi s n 17071, Girona, Spain
    Glycobiology 13:227-44. 2003
    Characterization of the N-glycans from human pancreatic ribonuclease (RNase 1) isolated from healthy pancreas and from pancreatic adenocarcinoma tumor cells (Capan-1 and MDAPanc-3) revealed completely different glycosylation patterns...
  6. ncbi Three-dimensional structure of human RNase 1 delta N7 at 1.9 A resolution
    J Pous
    Institut de Biologia Molecular de Barcelona, CID CSIC, Jordi Girona 18 26, 08034 Barcelona, Spain
    Acta Crystallogr D Biol Crystallogr 57:498-505. 2001
    Human pancreatic ribonuclease 1 (RNase 1) is considered to be the human counterpart of bovine pancreatic RNase A...
  7. ncbi The amino acid sequence of human pancreatic ribonuclease
    J J Beintema
    Anal Biochem 136:48-64. 1984
    The primary structure of human (Homo sapiens) pancreatic ribonuclease has been determined by automatic sequencing of the native protein and by analysis of peptides obtained by cleavage with proteolytic enzymes, cyanogen bromide, and ..
  8. ncbi Human endothelial cells selectively express large amounts of pancreatic-type ribonuclease (RNase 1)
    Julien B P Landré
    INSERM EPI 0113, Universite Bordeaux I, Avenue des Facultes, Talence, France
    J Cell Biochem 86:540-52. 2002
    ..The selective strong release of pancreatic-type RNase by endothelial cells suggests that it is endowed with non-digestive functions and involved in vascular homeostasis...
  9. ncbi Killing of endothelial cells and release of arachidonic acid. Synergistic effects among hydrogen peroxide, membrane-damaging agents, cationic substances, and proteinases and their modulation by inhibitors
    I Ginsburg
    Department of Oral Biology, Hebrew University Hadassah School of Medicine, Jerusalem, Israel
    Inflammation 17:295-319. 1993
    ..agents: the cationic substances, nuclear histone, defensins, lysozyme, poly-L-arginine, spermine, pancreatic ribonuclease, polymyxin B, chlorhexidine, cetyltrimethyl ammonium bromide, as well as by the membrane-damaging agents ..
  10. pmc Molecular evolution of B6 enzymes: binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzyme
    Rosa A Vacca
    Institute of Biomembranes and Bioenergetics, CNR, Via Amendola 165 A, I 70126 Bari, Italy
    BMC Biochem 9:17. 2008
    ..Here we report on an attempt to re-enact the emergence of a PLP-dependent protoenzyme. The starting protein was pancreatic ribonuclease A (RNase), in which active-site Lys41 or Lys7 readily form a covalent adduct with PLP.
  11. pmc Recognition of ribonuclease A by 3'-5'-pyrophosphate-linked dinucleotide inhibitors: a molecular dynamics/continuum electrostatics analysis
    Savvas Polydoridis
    Department of Physics, University of Cyprus, Nicosia, Cyprus
    Biophys J 92:1659-72. 2007
    The proteins of the pancreatic ribonuclease A (RNase A) family catalyze the cleavage of the RNA polymer chain...
  12. ncbi The phylogenetic position of "Acomyinae" (Rodentia, Mammalia) as sister group of a Murinae + Gerbillinae clade: evidence from the nuclear ribonuclease gene
    J Y Dubois
    Rijks Universiteit Groningen, Nijenborgh 4, Groningen, 9747 AG, The Netherlands
    Mol Phylogenet Evol 13:181-92. 1999
    The phylogenetic relationships of Acomys and Uranomys within Muridae were investigated using nuclear pancreatic ribonuclease A gene sequences...
  13. doi The molecular characterization of a novel GH38 α-mannosidase from the crenarchaeon Sulfolobus solfataricus revealed its ability in de-mannosylating glycoproteins
    Beatrice Cobucci-Ponzano
    Institute of Protein Biochemistry Consiglio Nazionale delle Ricerche, Via P Castellino 111, 80131 Naples, Italy
    Biochimie 92:1895-907. 2010
    ..More interestingly, Ssα-man removes mannose residues from the glycosidic moiety of the bovine pancreatic ribonuclease B, suggesting that it could process mannosylated proteins also in vivo...
  14. ncbi Activation pathways of 5-fluorouracil in rat organs and in PC12 cells
    L Mascia
    Department of Physiology and Biochemistry, University of Pisa, Laboratory of Biochemistry, Via S Maria 55, I 56126 Pisa, Italy
    Biochem Pharmacol 62:213-8. 2001
    ..of ribose 5-phosphate to 5-FU as catalysed by orotate phosphoribosyltransferase; 2) the ribose 1-phosphate (Rib1-P)-mediated addition of ribose by uridine phosphorylase, followed by the action of uridine kinase; and 3) the 2'-..
  15. pmc Onconase and amphinase, the antitumor ribonucleases from Rana pipiens oocytes
    W Ardelt
    Alfacell Corporation, 300 Atrium Drive, Somerset, NJ 08873, USA
    Curr Pharm Biotechnol 9:215-25. 2008
    Rana pipiens oocytes contain two homologues of pancreatic ribonuclease A that are cytostatic and cytotoxic to human cancer cells...
  16. doi Multiple solvent crystal structures of ribonuclease A: an assessment of the method
    Michelle Dechene
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695, USA
    Proteins 76:861-81. 2009
    ..The crystal structures of bovine pancreatic ribonuclease A (RNAse A) soaked in the following organic solvents are presented: 50% dioxane, 50% dimethylformamide, ..
  17. ncbi Purification and characterization of a ribonuclease from human liver
    S Sorrentino
    Department of Biological Chemistry, UCLA School of Medicine 90024 1737
    J Biol Chem 263:16125-31. 1988
    ..composition and the amino-terminal sequence of the enzyme indicate that the protein is related to human pancreatic ribonuclease and to angiogenin, and that it may be identical with an eosinophil-derived neurotoxin and to a ..
  18. ncbi Evolution of nucleic acids coding for ribonucleases: the mRNA sequence of mouse pancreatic ribonuclease
    C Schuller
    Biochemisch Laboratorium, Rijksuniversiteit Groningen, The Netherlands
    Mol Biol Evol 7:29-44. 1990
    ..Comparison with the rat pancreatic ribonuclease sequence showed a high rate of nucleotide substitution...
  19. pmc Natural merodiploidy of the lux-rib operon of Photobacterium leiognathi from coastal waters of Honshu, Japan
    Jennifer C Ast
    Department of Ecological and Evolutionary Biology, University of Michigan, 830 North University Ave, Ann Arbor, MI 48109 1048, USA
    J Bacteriol 189:6148-58. 2007
    ..13.1 defined two complete, physically separate, and apparently functional operons, designated lux-rib1 and lux-rib2. P. leiognathi strains lelon.2.1 and lnuch.21...
  20. ncbi Tandemization endows bovine pancreatic ribonuclease with cytotoxic activity
    Franziska Leich
    Department of Biochemistry and Biotechnology, Martin Luther University Halle Wittenberg, Kurt Mothes Str 3, 06120 Halle, Germany
    J Mol Biol 358:1305-13. 2006
    Due to their ability to degrade RNA, selected members of the bovine pancreatic ribonuclease A (RNase A) superfamily are potent cytotoxins...
  21. doi Modeling formalin fixation and histological processing with ribonuclease A: effects of ethanol dehydration on reversal of formaldehyde cross-links
    Carol B Fowler
    Department of Biophysics, Armed Forces Institute of Pathology, Rockville, MD 20850, USA
    Lab Invest 88:785-91. 2008
    ..Our initial studies of single proteins, such as bovine pancreatic ribonuclease A (RNase A), in 10% buffered formalin solution revealed that upon removal of excess formaldehyde, ..
  22. doi Metabolic engineering and classic selection of the yeast Candida famata (Candida flareri) for construction of strains with enhanced riboflavin production
    Kostyantyn V Dmytruk
    Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14 16, Lviv 79005, Ukraine
    Metab Eng 13:82-8. 2011
    ..factor SEF1 and IMH3 (coding for IMP dehydrogenase) orthologs from Debaryomyces hansenii, and the homologous genes RIB1 and RIB7, encoding GTP cyclohydrolase II and riboflavin synthetase, the first and the last enzymes of riboflavin ..
  23. doi Bactericidal activity engineered on human pancreatic ribonuclease and onconase
    Gerard Torrent
    Laboratori d Enginyeria de Proteïnes, Departament de Biologia, Facultat de Ciencies, Universitat de Girona, Campus de Montilivi s n 17071 Girona, Spain
    Mol Pharm 6:531-42. 2009
    ..In the present paper, however, we have used two nonbactericidal ribonucleases, onconase and the human pancreatic ribonuclease, as scaffolds onto which to engineer bactericidal activity...
  24. doi Adaptive evolution of digestive RNASE1 genes in leaf-eating monkeys revisited: new insights from ten additional colobines
    Li Yu
    Laboratory for Conservation and Utilization of Bio resource and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, China
    Mol Biol Evol 27:121-31. 2010
    ....
  25. pmc Trifluoroethanol-induced conformational transitions of proteins: insights gained from the differences between alpha-lactalbumin and ribonuclease A
    K Gast
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Protein Sci 8:625-34. 1999
    ..changes of two proteins widely used in folding experiments, bovine alpha-lactalbumin, and bovine pancreatic ribonuclease A, have been investigated...
  26. pmc Evasion of ribonuclease inhibitor as a determinant of ribonuclease cytotoxicity
    Thomas J Rutkoski
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Curr Pharm Biotechnol 9:185-9. 2008
    Onconase (ONC) is an amphibian member of the bovine pancreatic ribonuclease (RNase A) superfamily that exhibits innate antitumoral activity. ONC has been granted both orphan-drug and fast-track status by the U.S...
  27. pmc Antitumor activity of ribonuclease multimers created by site-specific covalent tethering
    Thomas J Rutkoski
    Departments of Biochemistry and Chemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Bioconjug Chem 21:1691-702. 2010
    ..A monomeric RI-evasive variant of bovine pancreatic ribonuclease (RNase A) inhibits the growth of human prostate and lung tumors in mice...
  28. ncbi The crystal structure of eosinophil cationic protein in complex with 2',5'-ADP at 2.0 A resolution reveals the details of the ribonucleolytic active site
    C Gopi Mohan
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, U K
    Biochemistry 41:12100-6. 2002
    ..ECP belongs to the pancreatic ribonuclease superfamily of proteins, and the crystal structure of ECP in the unliganded form (determined previously) ..
  29. ncbi Crystal structures of ribonuclease A complexes with 5'-diphosphoadenosine 3'-phosphate and 5'-diphosphoadenosine 2'-phosphate at 1.7 A resolution
    D D Leonidas
    School of Biology and Biochemistry, University of Bath, Claverton Down, U K
    Biochemistry 36:5578-88. 1997
    High-resolution (1.7 A) crystal structures have been determined for bovine pancreatic ribonuclease A (RNase A) complexed with 5'-diphosphoadenosine 3'-phosphate (ppA-3'-p) and 5'-diphosphoadenosine 2'-phosphate (ppA-2'-p), as well as for ..
  30. doi Electric charge divergence in proteins: insights into the evolution of their three-dimensional properties
    Yukuto Sato
    Division of Molecular Marine Biology, Ocean Research Institute, University of Tokyo, Minamidai 1 15 1, Nakano Ku, Tokyo 164 8639, Japan
    Gene 441:3-11. 2009
    ..To obtain further insights, ALD and PGI were compared to the proteins pancreatic ribonuclease (EC 3.1.27.5) and triose-phosphate isomerase (EC 5.3.1...
  31. ncbi Production of human pancreatic ribonuclease in Saccharomyces cerevisiae and Escherichia coli
    M Ribo
    Unitat de Bioquímica i Biologia Molecular, Departament de Biologia, Universitat de Girona, Girona, Spain
    Protein Expr Purif 7:253-61. 1996
    Human pancreatic ribonuclease (HP-RNase) has considerable promise as a therapeutic agent. Structure-function analyses of HP-RNase have been impeded by the difficulty of obtaining the enzyme from its host...
  32. doi Toward an antitumor form of bovine pancreatic ribonuclease: the crystal structure of three noncovalent dimeric mutants
    Antonello Merlino
    Department of Chemistry, University of Naples, Complesso Universitario Monte S Angelo, Via Cinthia, I 80126 Naples, Italy
    Biopolymers 91:1029-37. 2009
    ..The introduction of Leu28, Cys31, and Cys32 and, in addition, of Pro19 in the sequence of bovine pancreatic ribonuclease (RNase A) has produced two dimeric variants LCC and PLCC, which do exhibit a cytotoxic activity, though ..
  33. ncbi The structural determinants that lead to the formation of particular oligomeric structures in the pancreatic-type ribonuclease family
    A Benito
    Laboratori d Enginyeria de Proteïnes, Departament de Biologia, Facultat de Ciencies, Universitat de Girona, Campus de Montilivi s n, E 17071, Girona, Spain
    Curr Protein Pept Sci 9:370-93. 2008
    ..The prototype of this family is bovine pancreatic ribonuclease or ribonuclease A...
  34. pmc Tuning the pK(a) of fluorescein to optimize binding assays
    Luke D Lavis
    Departments of Chemistry and Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Anal Chem 79:6775-82. 2007
    ..The fluorescence of fluorescein-labeled model protein, bovine pancreatic ribonuclease (RNase A), decreases upon binding to its cognate inhibitor protein (RI)...
  35. ncbi Cytotoxicity of bovine seminal ribonuclease: monomer versus dimer
    J Eugene Lee
    Departments of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Biochemistry 44:15760-7. 2005
    Bovine seminal ribonuclease (BS-RNase) is a homologue of bovine pancreatic ribonuclease (RNase A). Unlike RNase A, BS-RNase has notable toxicity for human tumor cells...
  36. pmc Structural features for the mechanism of antitumor action of a dimeric human pancreatic ribonuclease variant
    Antonello Merlino
    Dipartimento di Chimica, Universita degli Studi di Napoli Federico II, Via Cintia, Napoli 80126, Italy
    Protein Sci 18:50-7. 2009
    ....
  37. ncbi A new mutant of bovine seminal ribonuclease with a reversed swapping propensity
    Carmine Ercole
    Dipartimento di Chimica, Universita Federico II di Napoli, Via Cintia, 80126 Napoli, Italy
    Biochemistry 46:2227-32. 2007
    ..mutants by replacing selected residues with the corresponding ones of its monomeric counterpart, bovine pancreatic ribonuclease (RNase A)...
  38. ncbi Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11
    S B delCardayre
    Department of Biochemistry, University of Wisconsin Madison 53706 1569, USA
    Protein Eng 8:261-73. 1995
    Bovine pancreatic ribonuclease A (RNase A) has been the object of much landmark work in biological chemistry...
  39. doi The structural basis of the difference in sensitivity for PNGase F in the de-N-glycosylation of the native bovine pancreatic ribonucleases B and BS
    Veronique Blanchard
    Bijvoet Center, Department of Bio Organic Chemistry, Utrecht University, Padualaan 8, NL 3584 CH Utrecht, The Netherlands
    Biochemistry 47:3435-46. 2008
    ..A typical example is native bovine pancreatic ribonuclease B (RNase B) with oligomannose-type N-glycans at Asn-34...
  40. pmc Oligomerization and aggregation of bovine pancreatic ribonuclease A: characteristic events observed by FTIR spectroscopy
    Yong Bin Yan
    Department of Biological Sciences and Biotechnology, and State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China
    Biophys J 90:2525-33. 2006
    ..We studied the specific events of bovine pancreatic ribonuclease A thermal aggregation by a combination of second derivative infrared analysis and two-dimensional ..
  41. ncbi Crystal structure of porcine ribonuclease inhibitor, a protein with leucine-rich repeats
    B Kobe
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235 9050
    Nature 366:751-6. 1993
    Ribonuclease inhibitor is a cytoplasmic protein that tightly binds and inhibits ribonucleases of the pancreatic ribonuclease superfamily...
  42. pmc Dissimilarity in the oxidative folding of onconase and ribonuclease A, two structural homologues
    Robert F Gahl
    Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853 1301, USA
    Protein Eng Des Sel 21:223-31. 2008
    ..A family, was examined and shows markedly different behavior compared to its structural homologue bovine pancreatic ribonuclease A (RNase A) under similar conditions...
  43. pmc Embryo transfer rederivation of C.B-17/Icr-Prkdc(scid) mice experimentally infected with mouse parvovirus 1
    David G Besselsen
    Department of University Animal Care, University of Arizona, Tucson, AZ, USA
    Comp Med 58:353-9. 2008
    ..All of these mice were negative by MFI for parvovirus nonstructural protein antigen (rNS1) and by qPCR, with the exception of 1 recipient female mouse that displayed weak rNS1 seroreactivity and low levels ..
  44. pmc 'Crystal lattice engineering,' an approach to engineer protein crystal contacts by creating intermolecular symmetry: crystallization and structure determination of a mutant human RNase 1 with a hydrophobic interface of leucines
    Hidenori Yamada
    Department of Medical and Bioengineering Science, Graduate School of Natural Science and Technology, Okayama University, Japan
    Protein Sci 16:1389-97. 2007
    ..interface (comprising four leucine residues) was introduced into a helical region (helix 2) of the human pancreatic ribonuclease 1 (RNase 1) that was predicted to form a suitable crystallization interface...
  45. ncbi Two-metal-ion mechanism for hammerhead-ribozyme catalysis
    Fabrice Leclerc
    Laboratoire de Maturation des ARN et Enzymologie Moleculaire, Universite Henri Poincare, Faculte des Sciences, 54506 Vandoeuvre les Nancy, France
    J Phys Chem B 110:3395-409. 2006
    ..Thus, the overall reaction corresponds to that catalyzed by bovine pancreatic ribonuclease. However, an essential distinguishing aspect is that metal ions are not involved in RNase catalysis but ..
  46. pmc Conformational changes below the Tm: molecular dynamics studies of the thermal pretransition of ribonuclease A
    Eric D Merkley
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 5061, USA
    Biochemistry 47:880-92. 2008
    ..variation, we have performed all-atom, explicit-solvent molecular dynamics simulations of bovine pancreatic ribonuclease A (RNase A) up to its melting temperature (Tm approximately 337 K)...
  47. doi Live cell fluorescence imaging for early expression and localization of RIB1 and RIB3 genes in Ashbya gossypii
    Sudeshna Sengupta
    Department of Biotechnology, Indian Institute of Technology, Chennai, India
    J Basic Microbiol 54:81-7. 2014
    Ashbya gossypii is a riboflavin overproducing filamentous fungus. RIB1 and RIB3 genes encode GTP-cyclohydrolase II (GCH II) and DHBP synthase, respectively, the two rate limiting enzymes of the riboflavin biosynthetic pathway...
  48. ncbi Temperature dependence of the backbone dynamics of ribonuclease A in the ground state and bound to the inhibitor 5'-phosphothymidine (3'-5')pyrophosphate adenosine 3'-phosphate
    Evgenii L Kovrigin
    Department of Chemistry, Yale University, P O Box 208107, New Haven, Connecticut 06520, USA
    Biochemistry 42:5279-91. 2003
    ..inhibitor 5'-phosphothymidine(3',5')pyrophosphate adenosine 3'-phosphate (pTppAp) with bovine pancreatic ribonuclease A (RNase A) was characterized by calorimetry and solution NMR spectroscopy...
  49. ncbi The structural and functional studies of His119 and His12 in RNase A via chemical modification
    Shahrokh Safarian
    Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
    J Protein Chem 22:643-54. 2003
    The histidyl residues of bovine pancreatic ribonuclease A (RNase A) play a crucial role in enzymatic activity...
  50. pmc Modeling formalin fixation and antigen retrieval with bovine pancreatic ribonuclease A: I-structural and functional alterations
    Vladimir K Rait
    Division of Biophysics, Department of Cellular Pathology and Genetics, Armed Forces Institute of Pathology, Rockville, MD 20850, USA
    Lab Invest 84:292-9. 2004
    ..We used biophysical techniques to investigate the effects of formaldehyde treatment on bovine pancreatic ribonuclease A (RNase A)...
  51. ncbi Candida famata (Debaryomyces hansenii) DNA sequences containing genes involved in riboflavin synthesis
    Andriy Y Voronovsky
    Institute of Cell Biology NASU, Drahomanov Street 14 16, Lviv 79005, Ukraine
    Yeast 21:1307-16. 2004
    Previously cloned Candida famata (Debaryomyces hansenii) strain VKM Y-9 genomic DNA fragments containing genes RIB1 (codes for GTP cyclohydrolase II), RIB2 (encodes specific reductase), RIB5 (codes for dimethylribityllumazine synthase), ..
  52. pmc Open interface and large quaternary structure movements in 3D domain swapped proteins: insights from molecular dynamics simulations of the C-terminal swapped dimer of ribonuclease A
    Antonello Merlino
    Centro Interdipartimentale Ricerca e Management, Complesso Ristrutturato S Andrea delle Dame, 80138, Naples, Italy
    Biophys J 88:2003-12. 2005
    Bovine pancreatic ribonuclease (RNase A) forms two three-dimensional (3D) domain swapped dimers...
  53. ncbi Intrachain disulfide bridges of bovine seminal ribonuclease
    A Di Donato
    Biochim Biophys Acta 579:303-13. 1979
    ..identical with those of the eight half-cystine residues of the strictly homologous chain of bovine pancreatic ribonuclease. The results reported show that these eight 'homologous' half-cystine residues pair in seminal ..
  54. ncbi Disruption of shape-complementarity markers to create cytotoxic variants of ribonuclease A
    Thomas J Rutkoski
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    J Mol Biol 354:41-54. 2005
    Onconase (ONC), an amphibian member of the bovine pancreatic ribonuclease A (RNase A) superfamily, is in phase III clinical trials as a treatment for malignant mesothelioma...
  55. ncbi Role of aspartic acid 121 in human pancreatic ribonuclease catalysis
    Deepak Gaur
    Immunochemistry Laboratory, National Institute of Immunology, New Delhi, India
    Mol Cell Biochem 275:95-101. 2005
    Bovine pancreatic ribonuclease (RNase A) is one of the most well studied enzymes of the ribonuclease family, unlike its human counterpart, the human pancreatic ribonuclease (HPR), whose physiological role in the body is not clearly ..
  56. ncbi [Conjugates of pancreatic ribonuclease and ligand-free human serum albumin]
    E A Zelepuga
    Biomed Khim 49:588-96. 2003
    ..A single dose administration of LFHSA-RNAse conjugates exhibited high antiviral activity in mice, infected with influenza A and influenza B viruses...
  57. doi Extracellular RNA liberates tumor necrosis factor-α to promote tumor cell trafficking and progression
    Silvia Fischer
    Department of Biochemistry, Medical School, Justus Liebig University, Giessen, Germany
    Cancer Res 73:5080-9. 2013
    ..Our findings establish a crucial role for eRNA in driving tumor progression, and they suggest applications for extracellular RNase1 as an antiinvasive regimen for cancer treatment...
  58. ncbi Enhanced immune response by amphotericin B following NS1 protein prime-oral recombinant Salmonella vaccine boost vaccination protects mice from dengue virus challenge
    Wen Tssann Liu
    Institute of Preventive Medicine, National Defense Medical Center, P O Box 90048 505, Neihu, Taipei, Taiwan
    Vaccine 24:5852-61. 2006
    ..strain expressing a secreted dengue virus type 2 non-structural NS1 and Yersinia pestis F1 (Caf1) fusion protein, rNS1:Caf1, was generated. Immunological evaluation was performed by prime-boost vaccine regimen...
  59. ncbi The amino acid sequence of mouse pancreatic ribonuclease. Extremely rapid evolutionary rates of the myomorph rodent ribonucleases
    J A Lenstra
    Eur J Biochem 98:399-408. 1979
    The complete amino acid sequence of mouse pancreatic ribonuclease has been determined by analysis of tryptic, chymotryptic, thermolytic and CNBr peptides and by automatic sequence analysis of the intact protein...
  60. ncbi Oxidative folding of bovine pancreatic ribonuclease A: insight into the overall catalysis of the refolding pathway by phosphate
    Lawrence K Low
    Baker Laboratory of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853 1301, USA
    J Protein Chem 21:19-27. 2002
    The effects of the strong stabilizing anion, phosphate, on the oxidative folding of bovine pancreatic ribonuclease A were examined. Phosphate was found to catalyze several steps involved in the oxidative folding process at pH 8...
  61. ncbi Atomic resolution structures of ribonuclease A at six pH values
    R Berisio
    Centro di Studio di Biocristallografia, CNR, Via Mezzocannone 6, I 80134 Napoli, Italy
    Acta Crystallogr D Biol Crystallogr 58:441-50. 2002
    ..Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1...
  62. ncbi Rat pancreatic ribonuclease messenger RNA. The nucleotide sequence of the entire mRNA and the derived amino acid sequence of the pre-enzyme
    R J MacDonald
    J Biol Chem 257:14582-5. 1982
    We have cloned via recombinant DNA technology the mRNA sequence of rat pancreatic ribonuclease, and have determined the entire nucleotide sequence of the mature message...
  63. ncbi Prediction of recurrence in low and intermediate risk non-muscle invasive bladder cancer by real-time quantitative PCR analysis: cDNA microarray results
    J Mares
    Charles University, Prague, Czech Republic
    Neoplasma 60:295-301. 2013
    ..Our results indicate that it may be possible to identify patients with a low and high risk of disease recurrence at an early stage using a molecular profile...
  64. ncbi The mouse angiogenin gene family: structures of an angiogenin-related protein gene and two pseudogenes
    W E Brown
    Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genomics 29:200-6. 1995
    Angiogenin, a homologue of pancreatic ribonuclease, is a potent inducer of blood vessel formation...
  65. ncbi Ribonuclease inhibitor protein of human erythrocytes: characterization, loss of activity in response to oxidative stress, and association with Heinz bodies
    M Moenner
    INSERM Unité 427, Développement Humain, Croissance et Différenciation, Faculté des Sciences Pharmaceutiques et Biologiques de Paris, France
    Blood Cells Mol Dis 24:149-64. 1998
    ..Each inhibitor forms an equimolar complex with bovine pancreatic ribonuclease A and is inactivated by treatment with the sulfhydryl reagent p-(hydroxymercuri)benzoate...
  66. doi Refolding of ribonuclease A monitored by real-time photo-CIDNP NMR spectroscopy
    Iain J Day
    Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX13QZ, UK
    J Biomol NMR 44:77-86. 2009
    ..Real-time photo-CIDNP measurements yield residue-specific kinetic data pertaining to the folding reaction, interpreted in terms of current knowledge of the folding of bovine pancreatic ribonuclease A.
  67. doi A simple micro-method for determining precise oligosaccharidic specificity of mannose-binding lectins
    Henri Debray
    Unité de Glycobiologie Structurale et Fonctionnelle UMR CNRS USTL n degrees 8576 Université des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq cedex, France
    Glycobiology 19:1417-26. 2009
    ..of a mixture of N-[(14)C]-acetylated glycoasparagines, prepared by exhaustive pronase digestion of bovine pancreatic ribonuclease B and N-[(14)C]-acetylation with [(14)C]-acetic anhydride and containing all the possible oligomannoside-..
  68. doi X-ray crystallographic studies of RNase A variants engineered at the most destabilizing positions of the main hydrophobic core: further insight into protein stability
    Katarzyna Kurpiewska
    Faculty of Chemistry, Jagiellonian University, Ingardena 3, Krakow 30 060, Poland
    Proteins 77:658-69. 2009
    ..the structural origin of decreased pressure and temperature stability, the crystal structure of bovine pancreatic ribonuclease A variants V47A, V54A, V57A, I81A, I106A, and V108A was solved at 1.4-2...
  69. pmc Inhibitor design for ribonuclease A: the binding of two 5'-phosphate uridine analogues
    Vicky G Tsirkone
    Institute of Organic and Pharmaceutical Chemistry, National Hellenic Research Foundation, 11635 Athens, Greece
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:671-7. 2009
    In the quest for the rational design of selective and potent inhibitors for members of the pancreatic ribonuclease A (RNase A) family of biomedical interest, the binding of uridine 5'-phosphate (U5P) and uridine 5'-diphosphate (UDP) to ..
  70. ncbi Effect of bovine seminal ribonuclease and bovine pancreatic ribonuclease A on bovine oocyte maturation
    T Slavik
    Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Czech Republic
    J Exp Zool 287:394-9. 2000
    ..The MxM form and RNase A at 5 microg/ml inhibited the maturation rate by 71 and 48%, respectively, but a less significant effect was ..
  71. doi Characterization of a sub-family of Arabidopsis genes with the SPX domain reveals their diverse functions in plant tolerance to phosphorus starvation
    Ke Duan
    State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China
    Plant J 54:965-75. 2008
    ..Over-expression of AtSPX1 increased the transcript levels of ACP5, RNS1 and PAP2 under both phosphate-sufficient and phosphate-deficient conditions, suggesting a potential transcriptional ..
  72. doi Identification and quantification of N-linked oligosaccharides released from glycoproteins: an inter-laboratory study
    Smita Thobhani
    Analytical Science, National Physical Laboratory, Teddington, Middlesex, TW11 0LW, UK
    Glycobiology 19:201-11. 2009
    ..human alpha1-acid glycoprotein (H alpha1), bovine alpha1-acid glycoprotein (B alpha1), bovine pancreatic ribonuclease B (RNaseB), and human serum immunoglobulin G (hIgG)...
  73. ncbi Identification of c-myc coding region determinant RNA sequences and structures cleaved by an RNase1-like endoribonuclease
    Alaeddin Tafech
    Chemistry Program, University of Northern British Columbia, 3333 University Way, Prince George, Canada BC V2N 4Z9
    Biochim Biophys Acta 1769:49-60. 2007
    ..These properties further distinguish the enzyme from previously described mammalian endonuclease that cleaves c-myc mRNA in vitro...
  74. ncbi Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values
    O Lequin
    Département de Chimie Synthèse Organique, URA 1308 du CNRS, Ecole Polytechnique, Palaiseau, France
    Eur J Biochem 250:712-26. 1997
    Human angiogenin is a member of the pancreatic ribonuclease superfamily that induces blood vessel formation. Its three-dimensional solution structure has been determined to high resolution by heteronuclear NMR spectroscopy...
  75. ncbi New anti-CD30 human pancreatic ribonuclease-based immunotoxin reveals strong and specific cytotoxicity in vivo
    Sandra Braschoss
    Institut fur Pathologie, Charité Campus Benjamin Franklin FU Berlin, Germany
    Leuk Lymphoma 48:1179-86. 2007
    ..an immunotoxin composed of a single chain variable fragment of a CD30 antibody fused to the human pancreatic ribonuclease, showing CD30-specific binding and ribonucleolytic activity resistant to the inhibitor RNasin...
  76. ncbi Role of unique basic residues of human pancreatic ribonuclease in its catalysis and structural stability
    Punyatirtha Dey
    Immunochemistry Laboratory, National Institute of Immunology, Aruna Asaf Ali Road, New Delhi 110067, India
    Biochem Biophys Res Commun 360:809-14. 2007
    Human pancreatic ribonuclease (HPR) and bovine RNase A belong to the RNase A superfamily and possess similar key structural and catalytic residues...
  77. pmc Structural basis for catalysis by onconase
    J Eugene Lee
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706 1544, USA
    J Mol Biol 375:165-77. 2008
    Onconase (ONC) is a homolog of bovine pancreatic ribonuclease (RNase A) from the frog Rana pipiens. ONC displays antitumoral activity and is in advanced clinical trials for the treatment of cancer...
  78. ncbi Increasing the potency of a cytotoxin with an arginine graft
    Stephen M Fuchs
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Protein Eng Des Sel 20:505-9. 2007
    Variants and homologs of bovine pancreatic ribonuclease (RNase A) can exhibit cytotoxic activity. This toxicity relies on cellular internalization of the enzyme. Residues Glu49 and Asp53 form an anionic patch on the surface of RNase A...
  79. pmc Molecular dynamics simulation of bovine pancreatic ribonuclease A-CpA and transition state-like complexes
    Elena Formoso
    Kimika Fakultatea, Euskal Herriko Unibertsitatea and Donostia International Physics Center DIPC, P K 1072, 20080 Donostia, Euskadi, Spain
    J Phys Chem B 114:7371-82. 2010
    ..In this study, we apply molecular dynamics simulations of bovine pancreatic ribonuclease A, an archetype ribonuclease, to study the conformational dynamics, structural relaxation, and ..
  80. ncbi Isolation of Saccharomyces cerevisiae RNase T1 hypersensitive (rns) mutants and genetic analysis of the RNS1/DSL1 gene
    Tomoki Ishikawa
    Department of Biotechnology, The University of Tokyo, Tokyo, Japan
    J Gen Appl Microbiol 51:73-82. 2005
    ..We identified the genes mutated in three of the rns mutants, rns1, rns2, and rns3, as DSL1, UMP1, and SEC17, respectively...
  81. pmc Human ribonuclease 4 (RNase 4): coding sequence, chromosomal localization and identification of two distinct transcripts in human somatic tissues
    H F Rosenberg
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 23:4290-5. 1995
    ..encoding human ribonuclease 4 (RNase 4) of the mammalian ribonuclease gene family, whose members include pancreatic ribonuclease, eosinophil-derived neurotoxin, eosinophil cationic protein and angiogenin...
  82. ncbi Lymphocyte recognition of human parvovirus B19 non-structural (NS1) protein: associations with occurrence of acute and chronic arthropathy?
    L A Mitchell
    Department of Pathology, University of British Columbia, BC Research Institute for Children s and Women s Health, Vancouver, Canada
    J Med Microbiol 50:627-35. 2001
    Immune recognition of recombinant parvovirus B19 non-structural (rNS1) protein was studied by immunoblot and lymphoproliferative assays in blood from the following B19 seropositive groups: B19 infected (n = 14), B19 exposed but non-..
  83. pmc Folding, quality control, and secretion of pancreatic ribonuclease in live cells
    Roger Geiger
    Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland
    J Biol Chem 286:5813-22. 2011
    ..The presence of N-glycans had little effect on the folding and secretion process...
  84. pmc Cellular uptake of ribonuclease A relies on anionic glycans
    Tzu Yuan Chao
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, United States
    Biochemistry 49:10666-73. 2010
    Bovine pancreatic ribonuclease (RNase A) can enter human cells, even though it lacks a cognate cell-surface receptor protein. Here, we report on the biochemical basis for its cellular uptake...
  85. ncbi High-level expression of three members of the murine angiogenin family in Escherichia coli and purification of the recombinant proteins
    D E Holloway
    Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, United Kingdom
    Protein Expr Purif 22:307-17. 2001
    Angiogenin (Ang) is a small basic protein which belongs to the pancreatic ribonuclease superfamily. It potently induces the formation of new blood vessels and has emerged as a promising anticancer target...
  86. doi Production of recombinant nonstructural 1 protein in Escherichia coli for early detection of Japanese encephalitis virus infection
    Nagesh K Tripathi
    Bioprocess Scale Up Facility, Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, India
    Microb Biotechnol 5:599-606. 2012
    ..The recombinant nonstructural 1 (rNS1) protein of Japanese encephalitis virus is a potential diagnostic as well as vaccine candidate...
  87. doi (1)H, (13)C, (15)N backbone and side-chain resonance assignments of rat angiogenin
    Chung Kyung Lee
    Division of Magnetic Resonance, Korea Basic Science Institute, 162 Yeongudanji Ro, Ochang, Cheongwon, Chungbuk, 363 883, Korea
    Biomol NMR Assign 7:89-92. 2013
    Angiogenin is an unusual member of the pancreatic ribonuclease superfamily that induces formation of new blood vessels and is a promising anti-cancer target...
  88. ncbi PEG chains increase aspermatogenic and antitumor activity of RNase A and BS-RNase enzymes
    Josef Matousek
    Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov 27721, Czech Republic
    J Control Release 82:29-37. 2002
    RNase A (bovine pancreatic ribonuclease) and BS-RNase (bovine seminal ribonuclease) are monomeric and dimeric enzymes, respectively, with aspermatogenic and antitumor activities...
  89. ncbi Amino acid sequence of the nonsecretory ribonuclease of human urine
    J J Beintema
    Biochemisch Laboratorium, Rijksuniversiteit, Groningen, The Netherlands
    Biochemistry 27:4530-8. 1988
    ..to belong to the same ribonuclease superfamily as the mammalian secretory ribonucleases, turtle pancreatic ribonuclease, and human angiogenin...
  90. doi Novel cerebrospinal fluid biomarkers of axonal degeneration in frontotemporal dementia
    Niklas Mattsson
    Clinical Neurochemistry Laboratory, Sahlgren s University Hospital Mölndal, S 431 80 Molndal, Sweden
    Mol Med Report 1:757-61. 2008
    ..19 Da) as well as its mono- and diphosphorylated forms (7738.16 Da and 7818.13 Da, respectively) and pancreatic ribonuclease (14566.33 Da)...
  91. pmc Interactions crucial for three-dimensional domain swapping in the HP-RNase variant PM8
    Pere Tubert
    Laboratori d Enginyeria de Proteïnes, Departament de Biologia, Facultat de Ciencies, Universitat de Girona, Campus de Montilivi, Maria Aurèlia Campmany, 69 E 17071 Girona, Spain
    Biophys J 101:459-67. 2011
    ..investigated the role on the dimerization process of different residues of a domain-swapped dimer human pancreatic ribonuclease variant...
  92. ncbi The interdependence between catalytic activity, conformational stability, and cytotoxicity of onconase
    Cindy Schulenburg
    Martin Luther University Halle Wittenberg, Halle, Germany
    Cancer Biol Ther 6:1233-9. 2007
    Onconase (ONC) is a cytotoxic ribonuclease of the pancreatic ribonuclease A superfamily isolated from oocytes or early embryos of the Northern leopard frog (Rana pipiens)...
  93. ncbi Nucleotide sequence encoding human pancreatic ribonuclease
    M Seno
    Department of Bioengineering Science, Faculty of Engineering, Okayama University, Japan
    Biochim Biophys Acta 1218:466-8. 1994
    A cDNA coding for human pancreatic ribonuclease was isolated from a pancreas cDNA library and sequenced...
  94. doi RNase A oligomerization through 3D domain swapping is favoured by a residue located far from the swapping domains
    Francesca Vottariello
    Dipartimento di Scienze della Vita e della Riproduzione, Sezione di Chimica Biologica, Facolta di Medicina e Chirurgia, University of Verona, Strada Le Grazie 8, I 37134 Verona, Italy
    Biochimie 93:1846-57. 2011
    Bovine pancreatic ribonuclease A forms 3D domain-swapped oligomers by lyophilization from 40% acetic acid solutions or if subjected to various thermally-induced denaturation procedures...
  95. ncbi The amino acid sequence of human ribonuclease 4, a highly conserved ribonuclease that cleaves specifically on the 3' side of uridine
    H M Zhou
    Center for Biochemical and Biophysical Sciences and Medicine, Boston, MA 02115
    Eur J Biochem 217:401-10. 1993
    ..but differs substantially from both of these proteins; the protein sequence has 43% identity with human pancreatic ribonuclease and 39% identity with human angiogenin, as compared to 35% identity between human angiogenin and ..
  96. doi Human pancreatic ribonuclease presents higher endonucleolytic activity than ribonuclease A
    M Rodriguez
    Laboratori d Enginyeria de Proteïnes, Departament de Biologia, Facultat de Ciencies, Universitat de Girona, Campus de Montilivi s n, 17071 Girona, Spain
    Arch Biochem Biophys 471:191-7. 2008
    ..cleavage shows that the enzyme does not act randomly and follows a more endonucleolytic pattern when compared to RNase A. The enzyme prefers the binding and cleavage of longer substrate molecules, especially when the phosphodiester ..
  97. pmc Two distinct secretory ribonucleases from human cerebrum: purification, characterization and relationships to other ribonucleases
    T Yasuda
    Department of Legal Medicine, Fukui Medical School, Japan
    Biochem J 296:617-25. 1993
    ..Two RNAases corresponding to HB-1 and HB-2 were identified in fresh cerebrospinal fluid...
  98. ncbi Eosinophil cationic protein cDNA. Comparison with other toxic cationic proteins and ribonucleases
    R L Barker
    Department of Immunology, Mayo Medical School, Rochester, MN 55905
    J Immunol 143:952-5. 1989
    ..The ECP nucleotide sequence shows similarity to EDN, rat pancreatic ribonuclease, and human angiogenin; all are members of the ribonuclease gene superfamily...
  99. ncbi Antibody-onconase conjugates: cytotoxicity and intracellular routing
    S M Rybak
    Bionanomics, LLC, 411 Walnut Street, Green Cove Springs, FL 32043, USA
    Curr Pharm Biotechnol 9:226-30. 2008
    Onconase, a member of the pancreatic ribonuclease A superfamily, is currently in Phase III clinical trials for treatment of unresectable malignant mesothelioma...
  100. pmc Functional role of glutamine 28 and arginine 39 in double stranded RNA cleavage by human pancreatic ribonuclease
    Md Tabish Rehman
    Immunochemistry Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India
    PLoS ONE 6:e17159. 2011
    Human pancreatic ribonuclease (HPR), a member of RNase A superfamily, has a high activity on double stranded (ds) RNA. By virtue of this activity HPR appears to be involved in the host-defense against pathogenic viruses...

Research Grants74

  1. FLAVOPROTEINS IN FATTY ACID METABOLISM
    Colin Thorpe; Fiscal Year: 2003
    ..and cooperates with protein disulfide isomerase in the generation of the correct disulfide pairings in pancreatic ribonuclease. The mechanism of the reductive half- reaction will be probed with a variety of reduced protein ..
  2. RIBONUCLEASES IN CANCER CHEMOTHERAPY
    RONALD RAINES; Fiscal Year: 2007
    The overall goal of the proposed research is to reveal how variants and homologs of bovine pancreatic ribonuclease (RNase A) promote tumor cell death...
  3. CHEMISTRY AND BIOLOGY OF RIBONUCLEASE A
    RONALD RAINES; Fiscal Year: 2004
    ..a new type of ribonuclease inhibitor; (5) function in vivo-determine the true physiological role of pancreatic ribonuclease and the cytosolic ribonuclease inhibitor protein (which binds to pancreatic ribonuclease with high ..
  4. RIBONUCLEASES IN CANCER CHEMOTHERAPY
    Ronald T Raines; Fiscal Year: 2010
    Onconase (ONC), which is an amphibian homologue of bovine pancreatic ribonuclease (RNase A), is now undergoing a human clinical trial for the treatment of cancer...
  5. Evolution of vertebrate sensory genes
    Jianzhi Zhang; Fiscal Year: 2010
    ..These studies will also help understand human smell and taste variations and disorders. ..
  6. Functional genomic approaches to duplicate gene evolution
    Jianzhi Zhang; Fiscal Year: 2010
    ..A clear understanding of these relationships helps clarify the exact molecular basis of human diseases, a necessary step in the treatment and prevention of these diseases. ..
  7. Functional genomic approaches to duplicate gene evolution
    Jianzhi Zhang; Fiscal Year: 2010
    ..A clear understanding of these relationships helps clarify the exact molecular basis of human diseases, a necessary step in the treatment and prevention of these diseases. ..
  8. Evolution of vertebrate sensory genes
    Jianzhi Zhang; Fiscal Year: 2009
    ..These studies will also help understand human smell and taste variations and disorders. ..
  9. RNases for understanding the origin of new gene function
    Jianzhi Zhang; Fiscal Year: 2006
    ..Eight members of the superfamily are known in humans, and they are pancreatic RNase (or RNase 1), eosinophil-derived neurotoxin (EDN or RNase 2), eosinophil cationic protein (ECP or RNase 3), RNase 4, ..
  10. RNases for understanding the origin of new gene function
    Jianzhi Zhang; Fiscal Year: 2007
    ..Eight members of the superfamily are known in humans, and they are pancreatic RNase (or RNase 1), eosinophil-derived neurotoxin (EDN or RNase 2), eosinophil cationic protein (ECP or RNase 3), RNase 4, ..
  11. RNases for understanding the origin of new gene function
    Jianzhi Zhang; Fiscal Year: 2003
    ..Eight members of the superfamily are known in humans, and they are pancreatic RNase (or RNase 1), eosinophil-derived neurotoxin (EDN or RNase 2), eosinophil cationic protein (ECP or RNase 3), RNase 4, ..
  12. Evolution of vertebrate sensory genes
    Jianzhi Zhang; Fiscal Year: 2007
    ..These studies will also help understand human smell and taste variations and disorders. ..
  13. SPECTROSCOPIC/KINETIC STUDIES OF FOLDING PATHWAYS
    HAROLD SCHERAGA; Fiscal Year: 2007
    ..g., the undesirable association of sickle-cell hemogloblin, or the induction of an oncogene product, whose properties involve a conformational change when only one amino acid residue in the sequence is changed. ..
  14. MOTION AND PROXIMITY RELATIONS IN POLYPEPTIDES
    HAROLD SCHERAGA; Fiscal Year: 1980
    ..Also EPR studies will be carried out on spin-labeled ribonuclease to determine the pathway(s) of folding of this protein from the unfolded form (both with and without the disulfide bonds intact)...
  15. INTERNAL BONDING IN PROTEINS
    HAROLD SCHERAGA; Fiscal Year: 1980
    ..These techniques are being applied to specific problems, involving the structures of polypeptides and proteins in solution, which are described in this proposal...
  16. MOTION AND PROXIMITY RELATIONS IN POLYPEPTIDES
    HAROLD SCHERAGA; Fiscal Year: 1990
    ..Each system is selected to answer a specific conformational question, e.g. the mechanism of folding of ribonuclease, and the structures of onc gene products and growth factors (related to cancer)...
  17. Protein Chemistry
    RONALD RAINES; Fiscal Year: 2007
    ..of chemical and biochemical tools to reveal new structure-function relationships in one particular protein, ribonuclease A. During the course of that work, we discovered that two peptides, one with a C-terminal phosophinothioester ..
  18. SPECTROSCOPIC/KINETIC STUDIES OF FOLDING PATHWAYS
    HAROLD SCHERAGA; Fiscal Year: 2003
    ..four-disulfide bovine pancreatic ribonuclease A, and its homologs, three-disulfide bovine angiogenin and frog onconase...
  19. SPECTROSCOPIC/KINETIC STUDIES OF FOLDING PATHWAYS
    HAROLD SCHERAGA; Fiscal Year: 2004
    ..four-disulfide bovine pancreatic ribonuclease A, and its homolog, three- disulfide bovine angiogenin...
  20. SPECTROSCOPIC/KINETIC STUDIES OF FOLDING PATHWAYS
    HAROLD SCHERAGA; Fiscal Year: 1999
    ..spectroscopy, and nonradiative excitation energy transfer to determine the pathways of folding of bovine pancreatic ribonuclease A. Ribonuclease A contains four disulfide bonds...