pancreatic lipase

Summary

Gene Symbol: pancreatic lipase
Description: pancreatic lipase
Alias: PNLIPD, PTL, pancreatic triacylglycerol lipase, triacylglycerol acylhydrolase
Species: human
Products:     pancreatic lipase

Top Publications

  1. van Tilbeurgh H, Sarda L, Verger R, Cambillau C. Structure of the pancreatic lipase-procolipase complex. Nature. 1992;359:159-62 pubmed
    Interfacial adsorption of pancreatic lipase is strongly dependent on the physical chemical properties of the lipid surface. These properties are affected by amphiphiles such as phospholipids and bile salts...
  2. Davis R, Diep A, Hunziker W, Klisak I, Mohandas T, Schotz M, et al. Assignment of human pancreatic lipase gene (PNLIP) to chromosome 10q24-q26. Genomics. 1991;11:1164-6 pubmed
    Human pancreatic lipase (EC 3.1.1.3) is a 56-kDa protein secreted by the acinar pancreas and is essential for the hydrolysis and absorption of long-chain triglyceride fatty acids in the intestine...
  3. Lowe M. Structure and function of pancreatic lipase and colipase. Annu Rev Nutr. 1997;17:141-58 pubmed
    ..This review discusses the advances made in protein structure and in understanding the relationships of structure to function of pancreatic triglyceride lipase and colipase. ..
  4. Winkler F, D Arcy A, Hunziker W. Structure of human pancreatic lipase. Nature. 1990;343:771-4 pubmed
    b>Pancreatic lipase (triacylglycerol acyl hydrolase) fulfills a key function in dietary fat absorption by hydrolysing triglycerides into diglycerides and subsequently into monoglycerides and free fatty acids...
  5. Khatua B, Trivedi R, Noel P, Patel K, Singh R, de Oliveira C, et al. Carboxyl Ester Lipase May Not Mediate Lipotoxic Injury during Severe Acute Pancreatitis. Am J Pathol. 2019;: pubmed publisher
    ..The pancreas expresses pancreatic triacylglycerol lipase (PNLIP), pancreatic lipase-related protein 2 (PNLIPRP2), and carboxyl ester lipase (CEL), which may ..
  6. Hachero Cruzado I, Rodríguez Rua A, Román Padilla J, Ponce M, Fernández Díaz C, Manchado M. Characterization of the genomic responses in early Senegalese sole larvae fed diets with different dietary triacylglycerol and total lipids levels. Comp Biochem Physiol Part D Genomics Proteomics. 2014;12:61-73 pubmed publisher
  7. Pramanik K, Saren S, Mitra S, Ghosh P, Maiti T. Computational elucidation of phylogenetic, structural and functional characteristics of Pseudomonas Lipases. Comput Biol Chem. 2018;74:190-200 pubmed publisher
    Lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) catalyzes tri-, di-, and monoacyl glycerol of fat into glycerol and fatty acids...
  8. Dong G, Liu W, Wu L, Yu D, Huang F, Li P, et al. Conjugated linoleic acid alters growth performance, tissue lipid deposition, and fatty acid composition of darkbarbel catfish (Pelteobagrus vachelli). Fish Physiol Biochem. 2015;41:73-89 pubmed publisher
    ..than those of fish fed with the control, and fish fed with 1-3 % CLA diets had significantly higher pancreatic triacylglycerol lipase activities in liver than those of fish fed with the control...
  9. Baek K, Park D, Hwang H, Kim S, Lee H, Baek J. Blocking ??/??-Adrenergic Signaling Reduces Dietary Fat Absorption by Suppressing Expression of Pancreatic Lipase in High Fat-Fed Mice. Int J Mol Sci. 2018;19: pubmed publisher
    We investigated whether ?-adrenergic antagonists attenuates dietary fat absorption through the regulation of pancreatic lipase (PNLIP) expression in pancreatic acinar cells in the context of high fat diet feeding...

More Information

Publications52

  1. Danino H, Naor R, Fogel C, Ben Harosh Y, Kadir R, Salem H, et al. PPAR? regulates exocrine pancreas lipase. Biochim Biophys Acta. 2016;1861:1921-1928 pubmed publisher
    b>Pancreatic lipase (triacylglycerol lipase EC 3.1.1.3) is an essential enzyme in hydrolysis of dietary fat...
  2. Xiao X, Lowe M. The β5-Loop and Lid Domain Contribute to the Substrate Specificity of Pancreatic Lipase-related Protein 2 (PNLIPRP2). J Biol Chem. 2015;290:28847-56 pubmed publisher
    ..lipase (PNLIP) is essential for dietary fat digestion in children and adults, whereas a homolog, pancreatic lipase-related protein 2 (PNLIPRP2), is critical in newborns...
  3. Wang Z, Xu S, Du K, Huang F, Chen Z, Zhou K, et al. Evolution of Digestive Enzymes and RNASE1 Provides Insights into Dietary Switch of Cetaceans. Mol Biol Evol. 2016;33:3144-3157 pubmed
    ..In sum, this is the first study that provides new insights into the evolutionary mechanism of dietary switch in cetaceans. ..
  4. Lai Y, Zhang F, Nayak T, Modarres R, Lee N, McCaffrey T. Detecting discordance enrichment among a series of two-sample genome-wide expression data sets. BMC Genomics. 2017;18:1050 pubmed publisher
    ..a series of non-overlapping subsets according to the tumor/non-tumor paired expression ratio of gene PNLIP (pancreatic lipase, recently shown it association with pancreatic cancer). The log-ratio ranges from a negative value (e.g...
  5. Kiba T. Changes of the expressions of multiple metabolism genes in rat pancreatic islets after ventromedial hypothalamic lesioning. Neurosci Lett. 2015;604:64-8 pubmed publisher
    ..reaction also confirmed that gene expressions of arachidonate 15-lipoxygenase (Alox15) was up-regulated and pancreatic lipase (Pnlip) was downregulated at day 3 after the VMH lesions...
  6. Doolittle M, Neher S, Ben Zeev O, Ling Liao J, Gallagher C, Hosseini M, et al. Lipase maturation factor LMF1, membrane topology and interaction with lipase proteins in the endoplasmic reticulum. J Biol Chem. 2009;284:33623-33 pubmed publisher
    ..We discuss the implication of our results with regard to lipase maturation and DUF1222 domain structure. ..
  7. Bang C, Kim J, Park S, Baik G, Su K, Yoon J, et al. Clinical efficacy of serum lipase subtype analysis for the differential diagnosis of pancreatic and non-pancreatic lipase elevation. Korean J Intern Med. 2016;31:660-8 pubmed publisher
    ..the clinical efficacy of serum lipase subtype analysis for the differential diagnosis of pancreatic and non-pancreatic lipase elevation. Patients who were referred for the serum lipase elevation were prospectively enrolled...
  8. Freie A, Ferrato F, Carriere F, Lowe M. Val-407 and Ile-408 in the beta5'-loop of pancreatic lipase mediate lipase-colipase interactions in the presence of bile salt micelles. J Biol Chem. 2006;281:7793-800 pubmed
  9. Ranaldi S, Belle V, Woudstra M, Bourgeas R, Guigliarelli B, Roche P, et al. Amplitude of pancreatic lipase lid opening in solution and identification of spin label conformational subensembles by combining continuous wave and pulsed EPR spectroscopy and molecular dynamics. Biochemistry. 2010;49:2140-9 pubmed publisher
    The opening of the lid that controls the access to the active site of human pancreatic lipase (HPL) was measured from the magnetic interaction between two spin labels grafted on this enzyme...
  10. Grandval P, De Caro A, De Caro J, Sias B, Carriere F, Verger R, et al. Critical evaluation of a specific ELISA and two enzymatic assays of pancreatic lipases in human sera. Pancreatology. 2004;4:495-503; discussion 503-4 pubmed
    Human pancreatic lipases (HPL) include the classical HPL, and two related proteins known as pancreatic lipase-related proteins 1 and 2 (HPLRP1 and 2)...
  11. Ramos P, Coste T, Piemont E, Lessinger J, Bousquet J, Chapus C, et al. Time-resolved fluorescence allows selective monitoring of Trp30 environmental changes in the seven-Trp-containing human pancreatic lipase. Biochemistry. 2003;42:12488-96 pubmed
    Human pancreatic lipase (HPL, triacylglycerol acylhydrolase, EC 3.1.1.3) is a carboxyl esterase which hydrolyzes insoluble emulsified triglycerides and is essential for the efficient digestion of dietary fats...
  12. Lessinger J, Arzoglou P, Ramos P, Visvikis A, Parashou S, Calam D, et al. Preparation and characterization of reference materials for human pancreatic lipase: BCR 693 (from human pancreatic juice) and BCR 694 (recombinant). Clin Chem Lab Med. 2003;41:169-76 pubmed
    ..An international certification campaign will be carried out to assign values to BCR 693 and BCR 694. ..
  13. Crandall W, Lowe M. Colipase residues Glu64 and Arg65 are essential for normal lipase-mediated fat digestion in the presence of bile salt micelles. J Biol Chem. 2001;276:12505-12 pubmed
    Pancreatic triglyceride lipase (PTL) requires colipase for activity. Various constituents in meals and in bile, particularly bile acids, inhibit PTL...
  14. Lowe M, Rosenblum J, Strauss A. Cloning and characterization of human pancreatic lipase cDNA. J Biol Chem. 1989;264:20042-8 pubmed
    Pancreatic lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) hydrolyzes dietary long chain triacylglycerol to free fatty acids and monoacylglycerols in the intestinal lumen...
  15. Sims H, Jennens M, Lowe M. The human pancreatic lipase-encoding gene: structure and conservation of an Alu sequence in the lipase gene family. Gene. 1993;131:281-5 pubmed
    The isolation and characterization of the human gene (hPL) encoding pancreatic lipase is reported. The gene has 13 exons dispersed in about 20 kb of genomic DNA. A pseudogene of hPL was also partially characterized...
  16. Giller T, Buchwald P, Blum Kaelin D, Hunziker W. Two novel human pancreatic lipase related proteins, hPLRP1 and hPLRP2. Differences in colipase dependence and in lipase activity. J Biol Chem. 1992;267:16509-16 pubmed
    We have isolated cDNAs coding for two novel human pancreatic lipase (hPL)-related human proteins, referred to as hPL-related proteins 1 and 2 (hPLRP1 and hPLRP2) and for hPL...
  17. Gyetvai G, Hughes T, Wedmore F, Roe C, Heikal L, Ghezzi P, et al. Erythropoietin Increases Myelination in Oligodendrocytes: Gene Expression Profiling Reveals Early Induction of Genes Involved in Lipid Transport and Metabolism. Front Immunol. 2017;8:1394 pubmed publisher
    ..Results were validated by quantitative PCR. These findings suggest that EPO might increase remyelination by inducing insulin-like growth factors and increasing lipid metabolism...
  18. Yang Y, Lowe M. Human pancreatic triglyceride lipase expressed in yeast cells: purification and characterization. Protein Expr Purif. 1998;13:36-40 pubmed
    ..Recombinant human pancreatic lipase was expressed in broth cultures and was purified from the medium by DEAE blue Sepharose and hydroxyapatite ..
  19. Carriere F, Thirstrup K, Boel E, Verger R, Thim L. Structure-function relationships in naturally occurring mutants of pancreatic lipase. Protein Eng. 1994;7:563-9 pubmed
    From primary structure comparison, the pancreatic lipase family is now divided into three subgroups: classical pancreatic lipases, pancreatic lipase-related proteins 1 (RPI) and pancreatic lipase-related proteins 2 (RP2)...
  20. Ranaldi S, Belle V, Woudstra M, Rodriguez J, Guigliarelli B, Sturgis J, et al. Lid opening and unfolding in human pancreatic lipase at low pH revealed by site-directed spin labeling EPR and FTIR spectroscopy. Biochemistry. 2009;48:630-8 pubmed publisher
    The structural changes induced in human pancreatic lipase (HPL) by lowering the pH were investigated using a combined approach involving the use of site-directed spin labeling coupled to electron paramagnetic resonance (SDSL-EPR) and ..
  21. Chahinian H, Sias B, Carriere F. The C-terminal domain of pancreatic lipase: functional and structural analogies with c2 domains. Curr Protein Pept Sci. 2000;1:91-103 pubmed
    The 3D structure of pancreatic lipase (PL) consists of two functional domains...
  22. Wadsäter M, Barauskas J, Tiberg F, Nylander T. The lipolytic degradation of highly structured cubic micellar nanoparticles of soy phosphatidylcholine and glycerol dioleate by phospholipase A2 and triacylglycerol lipase. Chem Phys Lipids. 2018;211:86-92 pubmed publisher
    ..PLA2) that catalyses degradation of the phospholipid component, SPC, and porcine pancreatic triacylglycerol lipase (TGL) that facilitate the hydrolysis of the diglyceride, GDO...
  23. Colin D, Deprez Beauclair P, Silva N, Infantes L, Kerfelec B. Modification of pancreatic lipase properties by directed molecular evolution. Protein Eng Des Sel. 2010;23:365-73 pubmed publisher
    ..evolution combined with rational design was used in the aim of improving the performances of the human pancreatic lipase at acidic pH. We set up a method for screening thousands of lipase variants for activity at low pH...
  24. Bénarouche A, Point V, Carriere F, Cavalier J. An interfacial and comparative in vitro study of gastrointestinal lipases and Yarrowia lipolytica LIP2 lipase, a candidate for enzyme replacement therapy. Biochimie. 2014;102:145-53 pubmed publisher
    ..All these findings support the use of YLLIP2 in enzyme replacement therapy for the treatment of pancreatic exocrine insufficiency, a pathological situation in which an acidification of intestinal contents occurs. ..
  25. van Tilbeurgh H, Egloff M, Martinez C, Rugani N, Verger R, Cambillau C. Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography. Nature. 1993;362:814-20 pubmed
    ..Together they form the lipid-water interface binding site. This reorganization of the lid structure provokes a second drastic conformational change in an active site loop, which in its turn creates the oxyanion hole (induced fit). ..
  26. Zhang G, He P, Tan H, Budhu A, Gaedcke J, Ghadimi B, et al. Integration of metabolomics and transcriptomics revealed a fatty acid network exerting growth inhibitory effects in human pancreatic cancer. Clin Cancer Res. 2013;19:4983-93 pubmed publisher
  27. Birari R, Bhutani K. Pancreatic lipase inhibitors from natural sources: unexplored potential. Drug Discov Today. 2007;12:879-89 pubmed
    ..for the treatment of obesity have involved inhibition of dietary triglyceride absorption via inhibition of pancreatic lipase (PL) as this is the major source of excess calories...
  28. Colin D, Deprez Beauclair P, Allouche M, Brasseur R, Kerfelec B. Exploring the active site cavity of human pancreatic lipase. Biochem Biophys Res Commun. 2008;370:394-8 pubmed publisher
    ..enzyme replacement therapy in cystic fibrosis, the feasibility of shifting the pH-activity profile of pancreatic lipase toward acidic values was investigated by site specific mutagenesis in different regions of the catalytic ..
  29. Roch A, Parikh J, Al Haddad M, Dewitt J, Ceppa E, House M, et al. Abnormal serum pancreatic enzymes, but not pancreatitis, are associated with an increased risk of malignancy in patients with intraductal papillary mucinous neoplasms. Surgery. 2014;156:923-9 pubmed publisher
    ..These data suggest serum pancreatic enzymes may be useful markers in stratification of pancreatic cancer risk in patients with IPMN. ..
  30. Yang Y, Jin L, Zhang J, Wang J, Zhao X, Wu G, et al. High HSF4 expression is an independent indicator of poor overall survival and recurrence free survival in patients with primary colorectal cancer. IUBMB Life. 2017;69:956-961 pubmed publisher
    ..We hypothesized that HSF4 might exert its oncogenic effects in CRC via some of these genes. © 2017 IUBMB Life, 69(12):956-961, 2017...
  31. Thomas A, Allouche M, Basyn F, Brasseur R, Kerfelec B. Role of the lid hydrophobicity pattern in pancreatic lipase activity. J Biol Chem. 2005;280:40074-83 pubmed
    b>Pancreatic lipase is a soluble globular protein that must undergo structural modifications before it can hydrolyze oil droplets coated with bile salts. The binding of colipase and movement of the lipase lid open access to the active site...
  32. Belle V, Fournel A, Woudstra M, Ranaldi S, Prieri F, Thomé V, et al. Probing the opening of the pancreatic lipase lid using site-directed spin labeling and EPR spectroscopy. Biochemistry. 2007;46:2205-14 pubmed
    Access to the active site of human pancreatic lipase (HPL) is controlled by a surface loop (the lid) that undergoes a conformational change in the presence of amphiphiles and lipid substrate...
  33. Bourbon Freie A, Dub R, Xiao X, Lowe M. Trp-107 and trp-253 account for the increased steady state fluorescence that accompanies the conformational change in human pancreatic triglyceride lipase induced by tetrahydrolipstatin and bile salt. J Biol Chem. 2009;284:14157-64 pubmed publisher
    The conformation of a surface loop, the lid, controls activity of pancreatic triglyceride lipase (PTL) by moving from a position that sterically hinders substrate access to the active site into a new conformation that opens and ..
  34. Lykidis A, Mougios V, Arzoglou P. Kinetics of the two-step hydrolysis of triacylglycerol by pancreatic lipases. Eur J Biochem. 1995;230:892-8 pubmed
    ..Using rabbit pancreatic lipase, we found that deoxycholate enhanced dioleoylglycerol hydrolysis to a higher degree than trioleoylglycerol ..
  35. Mann B, Goetz J, House M, Schmidt C, Novotny M. Glycomic and proteomic profiling of pancreatic cyst fluids identifies hyperfucosylated lactosamines on the N-linked glycans of overexpressed glycoproteins. Mol Cell Proteomics. 2012;11:M111.015792 pubmed publisher
    ..Several candidate glycoproteins that appear hyperfucosylated were identified, including triacylglycerol lipase and pancreatic ?-amylase, which were 20- and 22-fold more abundant, respectively, following A. aurantia lectin enrichment. ..
  36. Szabó A, Xiao X, Haughney M, Spector A, Sahin Tóth M, Lowe M. A novel mutation in PNLIP causes pancreatic triglyceride lipase deficiency through protein misfolding. Biochim Biophys Acta. 2015;1852:1372-9 pubmed publisher
    ..T221M. Furthermore, the ability of mutant p.T221M to induce ER stress suggests that this form of PNLIP deficiency might cause acinar cell damage as well. ..
  37. Behar D, Basel Vanagaite L, Glaser F, Kaplan M, Tzur S, Magal N, et al. Identification of a novel mutation in the PNLIP gene in two brothers with congenital pancreatic lipase deficiency. J Lipid Res. 2014;55:307-12 pubmed publisher
    Congenital pancreatic lipase (PNLIP) deficiency is a rare monoenzymatic form of exocrine pancreatic failure characterized by decreased absorption of dietary fat and greasy voluminous stools, but apparent normal development and an overall ..
  38. Javed S, Azeem F, Hussain S, Rasul I, Siddique M, Riaz M, et al. Bacterial lipases: A review on purification and characterization. Prog Biophys Mol Biol. 2018;132:23-34 pubmed publisher
    ..C.3.1.1.3) belongs to the hydrolases and is also known as fat splitting, glycerol ester hydrolase or triacylglycerol acylhydrolase. Lipase catalyzes the hydrolysis of triglycerides converting them to glycerol and fatty acids in an ..
  39. Camacho Ruiz M, Mateos Díaz J, Carriere F, Rodriguez J. A broad pH range indicator-based spectrophotometric assay for true lipases using tributyrin and tricaprylin. J Lipid Res. 2015;56:1057-67 pubmed publisher
    ..This continuous lipase assay is compatible with a high sample throughput and can be applied for the screening of lipases and lipase inhibitors from biological samples. ..
  40. Hegele R, Ramdath D, Ban M, Carruthers M, Carrington C, Cao H. Polymorphisms in PNLIP, encoding pancreatic lipase, and associations with metabolic traits. J Hum Genet. 2001;46:320-4 pubmed
    b>Pancreatic lipase (EC 3.1.1.3) is an exocrine secretion that hydrolyzes dietary triglycerides in the small intestine...
  41. Van Bennekum A, Fisher E, Blaner W, Harrison E. Hydrolysis of retinyl esters by pancreatic triglyceride lipase. Biochemistry. 2000;39:4900-6 pubmed
    ..Enzyme assays of pancreatic triglyceride lipase (PTL) showed that there was a colipase-stimulated REH activity in rat and mouse (WT and CELKO) pancreas, consistent with ..
  42. Mick E, McGough J, Deutsch C, Frazier J, Kennedy D, Goldberg R. Genome-wide association study of proneness to anger. PLoS ONE. 2014;9:e87257 pubmed publisher
    ..These results suggest that signaling pathways regulating intracellular calcium homeostasis, which are relevant to memory, learning, and neuronal survival, may in part underlie the expression of Angry Temperament. ..
  43. Egloff M, Marguet F, Buono G, Verger R, Cambillau C, van Tilbeurgh H. The 2.46 A resolution structure of the pancreatic lipase-colipase complex inhibited by a C11 alkyl phosphonate. Biochemistry. 1995;34:2751-62 pubmed
    b>Pancreatic lipase belongs to the serine esterase family and can therefore be inhibited by classical serine reagents such as diisopropyl fluoride or E600...