p85

Summary

Gene Symbol: p85
Description: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Alias: AGM7, GRB1, p85, p85-ALPHA, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, phosphatidylinositol 3-kinase regulatory subunit alpha, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha), phosphatidylinositol 3-kinase-associated p-85 alpha, phosphoinositide-3-kinase regulatory subunit, ptdIns-3-kinase regulatory subunit alpha
Species: human

Top Publications

  1. pmc Somatic mutations in p85alpha promote tumorigenesis through class IA PI3K activation
    Bijay S Jaiswal
    Department of Molecular Biology, Genentech Inc, South San Francisco, CA 94080, USA
    Cancer Cell 16:463-74. 2009
  2. ncbi Mechanism of two classes of cancer mutations in the phosphoinositide 3-kinase catalytic subunit
    Nabil Miled
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 317:239-42. 2007
  3. pmc PIK3R1 (p85α) is somatically mutated at high frequency in primary endometrial cancer
    Mary E Urick
    Cancer Genetics Branch, National Human Genome Research Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 71:4061-7. 2011
  4. ncbi Phosphatidylinositol 3-kinase in interleukin 1 signaling. Physical interaction with the interleukin 1 receptor and requirement in NFkappaB and AP-1 activation
    S A Reddy
    Department of Biochemistry and Molecular Biology, Box 117, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 272:29167-73. 1997
  5. pmc Direct positive regulation of PTEN by the p85 subunit of phosphatidylinositol 3-kinase
    Ryaz B Chagpar
    Department of Biochemistry, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada
    Proc Natl Acad Sci U S A 107:5471-6. 2010
  6. pmc Hypoxia up-regulates CD36 expression and function via hypoxia-inducible factor-1- and phosphatidylinositol 3-kinase-dependent mechanisms
    Bupe R Mwaikambo
    Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec H3G 1Y6, Canada
    J Biol Chem 284:26695-707. 2009
  7. ncbi HIV-1 Nef downregulates MHC-I by a PACS-1- and PI3K-regulated ARF6 endocytic pathway
    Anastassia D Blagoveshchenskaya
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Cell 111:853-66. 2002
  8. ncbi HIV-1-Tat protein activates phosphatidylinositol 3-kinase/ AKT-dependent survival pathways in Kaposi's sarcoma cells
    Maria Chiara Deregibus
    Cattedra di Nefrologia, Dipartimento di Medicina Interna Università di Torino, Centro Ricerca Medicina Sperimentale CeRMS, Italy
    J Biol Chem 277:25195-202. 2002
  9. ncbi HIV-1 gp120-induced TNF-{alpha} production by primary human macrophages is mediated by phosphatidylinositol-3 (PI-3) kinase and mitogen-activated protein (MAP) kinase pathways
    ChuHee Lee
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Leukoc Biol 78:1016-23. 2005
  10. pmc Human immunodeficiency virus type 1 Tat accelerates Kaposi sarcoma-associated herpesvirus Kaposin A-mediated tumorigenesis of transformed fibroblasts in vitro as well as in nude and immunocompetent mice
    Xiuying Chen
    Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, PR China
    Neoplasia 11:1272-84. 2009

Research Grants

  1. Nutritional Effects on Insulin Signaling and Action
    Marc Andre Cornier; Fiscal Year: 2007
  2. PTEN TUMOR SUPPRESSOR AND SIGNAL TRANSDUCTION
    Ramon Parsons; Fiscal Year: 2005
  3. Myocardial ischemic activation of the Akt pathway
    STEPHEN ARMSTRONG; Fiscal Year: 2007
  4. PI 3 KINASE ISOFORMS AND INSULIN ACTION
    C Kahn; Fiscal Year: 2003
  5. Novel Function of PI 3-Kinase in Calcium Regulation
    Ching Shih Chen; Fiscal Year: 2004
  6. ANG II AND IGF-1 INTERACTION IN CARDIOVASCULAR TISSUE
    James Sowers; Fiscal Year: 2003
  7. IFNARI SIGNALING THROUGH STAT3, PI 3 KINASE
    LAWRENCE PFEFFER; Fiscal Year: 2002
  8. PI 3-Kinase Isoforms in Insulin Action
    C Kahn; Fiscal Year: 2007
  9. PI 3-Kinase Isoforms in Insulin Action
    C Kahn; Fiscal Year: 2009
  10. PI 3-Kinase Isoforms in Insulin Action
    C Ronald Kahn; Fiscal Year: 2010

Detail Information

Publications225 found, 100 shown here

  1. pmc Somatic mutations in p85alpha promote tumorigenesis through class IA PI3K activation
    Bijay S Jaiswal
    Department of Molecular Biology, Genentech Inc, South San Francisco, CA 94080, USA
    Cancer Cell 16:463-74. 2009
    ..Oncogenic activating mutations in the p110alpha catalytic subunit of the heterodimeric p110/p85 PI3K enzyme are frequent in human cancers...
  2. ncbi Mechanism of two classes of cancer mutations in the phosphoinositide 3-kinase catalytic subunit
    Nabil Miled
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 317:239-42. 2007
    ..interface but in a polar surface patch that is a plausible docking site for other domains in the holo p110/p85 complex...
  3. pmc PIK3R1 (p85α) is somatically mutated at high frequency in primary endometrial cancer
    Mary E Urick
    Cancer Genetics Branch, National Human Genome Research Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 71:4061-7. 2011
    ..The goal of this study was to determine whether PIK3R1, which encodes p85α, the inhibitory subunit of PI3K, is mutated in endometrial carcinoma...
  4. ncbi Phosphatidylinositol 3-kinase in interleukin 1 signaling. Physical interaction with the interleukin 1 receptor and requirement in NFkappaB and AP-1 activation
    S A Reddy
    Department of Biochemistry and Molecular Biology, Box 117, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 272:29167-73. 1997
    ..3-kinase (PI 3-kinase) activity but also induces the physical interaction of its type I receptor with the p85 regulatory subunit of PI 3-kinase...
  5. pmc Direct positive regulation of PTEN by the p85 subunit of phosphatidylinositol 3-kinase
    Ryaz B Chagpar
    Department of Biochemistry, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada
    Proc Natl Acad Sci U S A 107:5471-6. 2010
    ..We demonstrate that ectopically expressed FLAG-tagged p85 coimmunoprecipitates endogenous PTEN in an epidermal growth factor dependent manner...
  6. pmc Hypoxia up-regulates CD36 expression and function via hypoxia-inducible factor-1- and phosphatidylinositol 3-kinase-dependent mechanisms
    Bupe R Mwaikambo
    Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec H3G 1Y6, Canada
    J Biol Chem 284:26695-707. 2009
    ..Together, our findings indicate a novel mechanism by which hypoxia induces CD36 expression via activation of HIF-1 and the phosphatidylinositol 3-kinase pathway...
  7. ncbi HIV-1 Nef downregulates MHC-I by a PACS-1- and PI3K-regulated ARF6 endocytic pathway
    Anastassia D Blagoveshchenskaya
    Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Cell 111:853-66. 2002
    ..These data provide new insights into the cellular basis of HIV-1 immunoevasion...
  8. ncbi HIV-1-Tat protein activates phosphatidylinositol 3-kinase/ AKT-dependent survival pathways in Kaposi's sarcoma cells
    Maria Chiara Deregibus
    Cattedra di Nefrologia, Dipartimento di Medicina Interna Università di Torino, Centro Ricerca Medicina Sperimentale CeRMS, Italy
    J Biol Chem 277:25195-202. 2002
    ....
  9. ncbi HIV-1 gp120-induced TNF-{alpha} production by primary human macrophages is mediated by phosphatidylinositol-3 (PI-3) kinase and mitogen-activated protein (MAP) kinase pathways
    ChuHee Lee
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Leukoc Biol 78:1016-23. 2005
    ..These gp120-triggered signaling pathways may be responsible for inappropriate production of proinflammatory cytokines by macrophages, which are believed to play a role in immunopathogenesis and in neurological sequelae of AIDS...
  10. pmc Human immunodeficiency virus type 1 Tat accelerates Kaposi sarcoma-associated herpesvirus Kaposin A-mediated tumorigenesis of transformed fibroblasts in vitro as well as in nude and immunocompetent mice
    Xiuying Chen
    Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, PR China
    Neoplasia 11:1272-84. 2009
    ....
  11. ncbi Determination of Gab1 (Grb2-associated binder-1) interaction with insulin receptor-signaling molecules
    S Rocchi
    INSERM U145, Nice, France
    Mol Endocrinol 12:914-23. 1998
    ..These include the regulatory subunit p85 of phosphatidylinositol 3-kinase and phosphotyrosine phosphatase, SHP-2...
  12. ncbi Functional association of platelet endothelial cell adhesion molecule-1 and phosphoinositide 3-kinase in human neutrophils
    F Pellegatta
    Laboratory of Cardiovascular Pathophysiology, Department of Cardiology, Scientific Institute San Raffaele, Instituto di Ricovero e Cura a Carattere Scientifico, I 20132 Milan, Italy
    J Biol Chem 273:27768-71. 1998
    ..In conclusion, PECAM-1/CD31 appears to be one of the molecules functionally coupled to PI3K, suggesting that this enzyme may represent a common pathway of integrin and adhesiveness regulation in leukocytes...
  13. ncbi Interaction of the Flt-1 tyrosine kinase receptor with the p85 subunit of phosphatidylinositol 3-kinase. Mapping of a novel site involved in binding
    S A Cunningham
    Department of Pharmacology, Texas Biotechnology Corporation, Houston 77030, USA
    J Biol Chem 270:20254-7. 1995
    We have examined the interactions of the p85 regulatory subunit of phosphatidylinositol 3-kinase with the endothelium-specific Flt-1 receptor tyrosine kinase using the yeast two-hybrid system...
  14. ncbi A Grb2-associated docking protein in EGF- and insulin-receptor signalling
    M Holgado-Madruga
    Department of Microbiology and Immunology, Jefferson Cancer Institute, Philadelphia, Pennsylvania 19107, USA
    Nature 379:560-4. 1996
    ..Over-expression of Gab1 enhances cell growth and results in transformation. We conclude that Gab1 is a new protein in EGF and insulin receptor signalling which could integrate signals from different systems...
  15. ncbi Crystal structure of the PI 3-kinase p85 amino-terminal SH2 domain and its phosphopeptide complexes
    R T Nolte
    Howard Hughes Medical Institute and Laboratory of Molecular Medicine, Children s Hospital, Boston, Massachusetts 02115, USA
    Nat Struct Biol 3:364-74. 1996
    ..The structures suggest a mechanism for the biological specificity exhibited by PI 3-kinase in its interactions with phosphoprotein partners...
  16. ncbi Tyrosine residue 719 of the c-kit receptor is essential for binding of the P85 subunit of phosphatidylinositol (PI) 3-kinase and for c-kit-associated PI 3-kinase activity in COS-1 cells
    H Serve
    Molecular Biology Program, Sloan Kettering Institute, New York, New York
    J Biol Chem 269:6026-30. 1994
    ..of the immediate events after binding of the kit ligand to the receptor is its association with the 85-kDa subunit (p85) of the phosphatidylinositol (PI) 3-kinase and the activation of the enzyme...
  17. pmc Grb2-associated binder-1 mediates phosphatidylinositol 3-kinase activation and the promotion of cell survival by nerve growth factor
    M Holgado-Madruga
    Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, 233 South 10th Street, Bluemle Life Science Building 1002, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 94:12419-24. 1997
    ..Hence, Gab1 has a major role in connecting TrkA with PI 3-kinase activation and for the promotion of cell survival by NGF...
  18. ncbi Type 1 TNF receptor forms a complex with and uses Jak2 and c-Src to selectively engage signaling pathways that regulate transcription factor activity
    Roxana Pincheira
    Department of Surgery and Comprehensive Cancer Center, University of California, San Francisco, CA 94115, USA
    J Immunol 181:1288-98. 2008
    ..These observations show that TNFR1 associates with and uses nonreceptor tyrosine kinases to engage signaling pathways, activate transcription factors, and modulate gene expression in cells...
  19. pmc The phosphoinositide 3-kinase regulatory subunit p85alpha can exert tumor suppressor properties through negative regulation of growth factor signaling
    Cullen M Taniguchi
    Joslin Diabetes Center and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02215, USA
    Cancer Res 70:5305-15. 2010
    Phosphoinositide 3-kinase (PI3K) plays a critical role in tumorigenesis, and the PI3K p85 regulatory subunit exerts both positive and negative effects on signaling...
  20. pmc Interaction of oestrogen receptor with the regulatory subunit of phosphatidylinositol-3-OH kinase
    T Simoncini
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 407:538-41. 2000
    ..Our findings define a physiologically important non-nuclear oestrogen-signalling pathway involving the direct interaction of ER alpha with PI(3)K...
  21. ncbi Tyrosine phosphorylation of p85 relieves its inhibitory activity on phosphatidylinositol 3-kinase
    B D Cuevas
    Division of Medicine, Department of Molecular Therapeutics, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 276:27455-61. 2001
    Under resting conditions, the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K) serves to both stabilize and inactivate the p110 catalytic subunit...
  22. pmc Novel PI3K/Akt inhibitors screened by the cytoprotective function of human immunodeficiency virus type 1 Tat
    Yuri Kim
    Department of Pharmacy, Kyung Hee University, Seoul, South Korea
    PLoS ONE 6:e21781. 2011
    ..Compound K inhibited Akt phosphorylation. This study supports that Tat-expressing CHME5 cells are an effective model system for screening novel PI3K/Akt inhibitors...
  23. ncbi Insulin-like growth factor-1 potentiates platelet activation via the IRS/PI3Kalpha pathway
    Ingeborg Hers
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, UK
    Blood 110:4243-52. 2007
    ..tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1) and IRS-2 and their association with the p85 subunit of phosphoinositide-3 kinase (PI3K)...
  24. pmc Raptor binds the SAIN (Shc and IRS-1 NPXY binding) domain of insulin receptor substrate-1 (IRS-1) and regulates the phosphorylation of IRS-1 at Ser-636/639 by mTOR
    Alexandros Tzatsos
    Massachusetts General Hospital Cancer Center, Boston, Massachusetts 02114, USA
    J Biol Chem 284:22525-34. 2009
    ..Overall, these data provide new insights in the molecular mechanisms by which mTORC1 inhibits PI 3-kinase/Akt signaling at the level of IRS-1 and suggest that mTOR signaling toward Akt is scaffold-dependent...
  25. ncbi Human immunodeficiency virus type 1 tat-mediated cytotoxicity of human brain microvascular endothelial cells
    Naveed Ahmed Khan
    Division of Pediatric Infectious Diseases, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Neurovirol 9:584-93. 2003
    ..Further understanding and characterization of Tat-HBMEC interactions should help us understand HIV-1 neuropathogenesis and develop strategies to prevent HIV-1 encephalopathy...
  26. pmc The v-Src SH3 domain binds phosphatidylinositol 3'-kinase
    X Liu
    Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Mol Cell Biol 13:5225-32. 1993
    ..Furthermore, the v-Src SH3 domain bound in vitro to the amino-terminal region of the p85 alpha subunit of PI 3'-kinase...
  27. pmc Signaling mechanism of HIV-1 gp120 and virion-induced IL-1beta release in primary human macrophages
    Ricky Cheung
    Pulmonary, Allergy, and Critical Care, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Immunol 180:6675-84. 2008
    ..This study identifies a novel signaling mechanism for HIV-1-induced IL-1beta production by primary human macrophages that may be involved in the neuropathogenesis of HIV-associated dementia...
  28. ncbi Growth hormone, interferon-gamma, and leukemia inhibitory factor promoted tyrosyl phosphorylation of insulin receptor substrate-1
    L S Argetsinger
    Department of Physiology, University of Michigan Medical School, Ann Arbor 48109 0622, USA
    J Biol Chem 270:14685-92. 1995
    ....
  29. ncbi Catalytically active TYK2 is essential for interferon-beta-mediated phosphorylation of STAT3 and interferon-alpha receptor-1 (IFNAR-1) but not for activation of phosphoinositol 3-kinase
    M R Rani
    Department of Neurosciences, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 274:32507-11. 1999
    ..KR930 cells, but evident in U1.wt cells. In U1A-derived cells, the p85/p110 phosphoinositol 3-kinase isoform was associated with IFNAR-1 but not STAT3, and the association was ligand-..
  30. ncbi Human ltk receptor tyrosine kinase binds to PLC-gamma 1, PI3-K, GAP and Raf-1 in vivo
    H Kozutsumi
    Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan
    Oncogene 9:2991-8. 1994
    ..wild-type ltk protein was tyrosine-phosphorylated in vivo and associated with SH2 containing proteins, PLC-gamma 1, p85 subunit of PI3-K and GAP, in vivo...
  31. pmc Small molecule inhibition of HIV-1-induced MHC-I down-regulation identifies a temporally regulated switch in Nef action
    Jimmy D Dikeakos
    Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Mol Biol Cell 21:3279-92. 2010
    ..Together, these studies resolve the seemingly controversial models that describe Nef-induced MHC-I down-regulation and provide new insights into the mechanism of Nef action...
  32. ncbi LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation
    W Zhang
    Section on Lymphocyte Signaling, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 5430, USA
    Cell 92:83-92. 1998
    ..36-38 kDa protein, previously characterized by its association with Grb2, phospholipase C-gamma1, and the p85 subunit of phosphoinositide 3-kinase...
  33. pmc Activation of phosphatidylinositol 3-kinase in response to interleukin-1 leads to phosphorylation and activation of the NF-kappaB p65/RelA subunit
    N Sizemore
    Department of Molecular Biology, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Mol Cell Biol 19:4798-805. 1999
    ..We have found that IL-1 stimulates interaction of the IL-1 receptor accessory protein with the p85 regulatory subunit of PI3K, leading to the activation of the p110 catalytic subunit...
  34. ncbi Hyperosmotic stress inhibits insulin receptor substrate-1 function by distinct mechanisms in 3T3-L1 adipocytes
    Philippe Gual
    INSERM U 568 and l Institut Fédératif de Recherches 50, Faculte de Medecine, Avenue de Valombrose, 06107 Nice Cedex 02, France
    J Biol Chem 278:26550-7. 2003
    ..On the other hand, prolonged osmotic stress alters IRS-1 function by inducing its degradation, which could contribute to the down-regulation of insulin action...
  35. doi Akt/Nox2/NF-κB signaling pathway is involved in Tat-induced HIV-1 long terminal repeat (LTR) transactivation
    Hong Sheng Zhang
    Beijing University of Technology, District of Chaoyang, China
    Arch Biochem Biophys 505:266-72. 2011
    ....
  36. ncbi HIV-1 Nef associated PAK and PI3-kinases stimulate Akt-independent Bad-phosphorylation to induce anti-apoptotic signals
    D Wolf
    Department of Dermatology Erlangen, University of Erlangen Nurnberg, Erlangen, Germany
    Nat Med 7:1217-24. 2001
    ..Nef anti-apoptotic effects are likely a crucial mechanism for viral replication in the host and thus in AIDS pathogenesis...
  37. ncbi The p85alpha subunit of phosphatidylinositol 3'-kinase binds to and stimulates the GTPase activity of Rab proteins
    M Dean Chamberlain
    Cancer Research Unit, Health Research Division, Saskatchewan Cancer Agency, Saskatoon, Saskatchewan S7N 4H4, Canada
    J Biol Chem 279:48607-14. 2004
    ..Cells expressing BH domain mutants of p85 show a reduced rate of PDGFR degradation as compared with wild type p85 expressing cells...
  38. ncbi Differential signaling by the epidermal growth factor-like growth factors neuregulin-1 and neuregulin-2
    C S Crovello
    Division of Signal Transduction, Beth Israel Deaconess Medical Center and the Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 273:26954-61. 1998
    ..Since both of these cell lines express only two of the known ErbB receptors, our results imply that EGF-like ligands might elicit differential signaling within the context of a single receptor heterodimer...
  39. ncbi HIV-1 Tat induces tyrosine phosphorylation of p125FAK and its association with phosphoinositide 3-kinase in PC12 cells
    D Milani
    Department of Morphology and Embriology, University of Ferrara, Italy
    AIDS 12:1275-84. 1998
    ..To evaluate the signal transduction potential of HIV-1 Tat in a neuronal cell model...
  40. ncbi Expression of a mutated form of the p85alpha regulatory subunit of phosphatidylinositol 3-kinase in a Hodgkin's lymphoma-derived cell line (CO)
    M Jucker
    Institut fur Medizinische Biochemie und Molekularbiologie, Abteilung für Zelluläre Signaltransduktion, Universitatsklinikum Hamburg Eppendorf, Universitat Hamburg, Martinistrasse 52, 20246 Hamburg, Germany
    Leukemia 16:894-901. 2002
    ..PI3-kinase is a heterodimer consisting of an 85 kDa adapter protein (p85) containing one SH3 domain and two SH2 domains and a 110 kDa catalytic subunit (p110)...
  41. ncbi Role of phosphoinositide 3-OH kinase in cell transformation and control of the actin cytoskeleton by Ras
    P Rodriguez-Viciana
    Imperial Cancer Research Fund, London, United Kingdom
    Cell 89:457-67. 1997
    ..The ability of activated Ras to stimulate PI 3-kinase in addition to Raf is therefore important in Ras transformation of mammalian cells and essential in Ras-induced cytoskeletal reorganization...
  42. pmc Differential association of phosphatidylinositol 3-kinase, SHIP-1, and PTEN with forming phagosomes
    Lynn A Kamen
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 0620, USA
    Mol Biol Cell 18:2463-72. 2007
    ..SHIP1-YFP and p85-YFP were recruited to forming phagosomes...
  43. ncbi Small GTPases and tyrosine kinases coregulate a molecular switch in the phosphoinositide 3-kinase regulatory subunit
    Tung O Chan
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Cancer Cell 1:181-91. 2002
    Phosphoinositide 3-kinase (PI3K) type IA is a heterodimer of a catalytic subunit, p110, and a regulatory subunit, p85. Here we show that p85 contains a GTPase-responsive domain and an inhibitory domain, which together form a molecular ..
  44. pmc The p85 regulatory subunit of phosphoinositide 3-kinase down-regulates IRS-1 signaling via the formation of a sequestration complex
    Ji Luo
    Department of Systems Biology, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA
    J Cell Biol 170:455-64. 2005
    ..The p85 regulatory subunit of PI 3-kinase is required to mediate the insulin-dependent recruitment of PI 3-kinase to the ..
  45. ncbi Constitutive association of SHP-1 with leukocyte-associated Ig-like receptor-1 in human T cells
    J G Sathish
    Department of Medicine, University of Wales College of Medicine, Cardiff, Wales, United Kingdom
    J Immunol 166:1763-70. 2001
    ..We propose that this mechanism may exert a constitutive negative regulatory role upon T cell signaling...
  46. pmc Cancer-derived mutations in the regulatory subunit p85alpha of phosphoinositide 3-kinase function through the catalytic subunit p110alpha
    Minghao Sun
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 107:15547-52. 2010
    ..Studies with isoform-specific inhibitors of p110 suggest that expression of p85 mutants in fibroblasts leads exclusively to an activation of p110alpha, and p110alpha is the sole mediator of p85 ..
  47. ncbi NMR trial models: experiences with the colicin immunity protein Im7 and the p85alpha C-terminal SH2-peptide complex
    R A Pauptit
    AstraZeneca, Mereside, Alderley Park, Macclesfield, Cheshire SK10 4TG, England
    Acta Crystallogr D Biol Crystallogr 57:1397-404. 2001
    ..Use of maximum-likelihood refinement proved critical to be able to refine the poor starting models. The challenges typical of the use of NMR trial models in molecular replacement are discussed...
  48. ncbi Purification and characterization of phosphoinositide 3-kinase from rat liver
    C L Carpenter
    Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111
    J Biol Chem 265:19704-11. 1990
    ..Substitution of Mn2+ for Mg2+ results in only approximately 10% of the Mg2(+)-dependent activity. Physiological calcium concentrations have no effect on the enzyme activity. Phosphoinositide 3-kinase has a broad pH optimum around 7...
  49. ncbi Interaction of the molecular weight 85K regulatory subunit of the phosphatidylinositol 3-kinase with the insulin receptor and the insulin-like growth factor-1 (IGF- I) receptor: comparative study using the yeast two-hybrid system
    S Tartare-Deckert
    INSERM, Nice Cedex 2, France
    Endocrinology 137:1019-24. 1996
    ..have proposed that the two receptors interact directly with the SH2 domains of the Mr 85K regulatory subunit (p85) of PI 3- kinase via phosphorylated Y1322THM and Y1316AHM sequences located in the carboxyl-terminal domain of the ..
  50. pmc Nutrients suppress phosphatidylinositol 3-kinase/Akt signaling via raptor-dependent mTOR-mediated insulin receptor substrate 1 phosphorylation
    Alexandros Tzatsos
    Boston University School of Medicine, Massachusetts 02118, USA
    Mol Cell Biol 26:63-76. 2006
    ..Thus, diabetes-related hyperglycemia hyperactivates the mTOR pathway and may lead to insulin resistance due to suppression of IRS-1-dependent PI3-kinase/Akt signaling...
  51. ncbi HIV-1 Nef blocks transport of MHC class I molecules to the cell surface via a PI 3-kinase-dependent pathway
    S A Swann
    Departments of Medicine and Microbiology and Immunology, The University of Michigan, Ann Arbor, Michigan 48109, USA
    Virology 282:267-77. 2001
    ..We propose that Nef diverts MHC-1 proteins into a PI 3-kinase-dependent transport pathway that prevents expression on the cell surface...
  52. ncbi Dual phosphorylation of phosphoinositide 3-kinase adaptor Grb2-associated binder 2 is responsible for superoxide formation synergistically stimulated by Fc gamma and formyl-methionyl-leucyl-phenylalanine receptors in differentiated THP-1 cells
    Haruka Momose
    Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan
    J Immunol 171:4227-34. 2003
    The class Ia phosphoinositide (PI) 3-kinase consisting of p110 catalytic and p85 regulatory subunits is activated by Tyr kinase-linked membrane receptors such as FcgammaRII through the association of p85 with the phosphorylated receptors ..
  53. ncbi HIV-1 Nef plays an essential role in two independent processes in CD4 down-regulation: dissociation of the CD4-p56(lck) complex and targeting of CD4 to lysosomes
    Y H Kim
    Laboratory of Molecular Virology, Samsung Biomedical Research Institute, Seoul, Korea
    Virology 257:208-19. 1999
    ....
  54. ncbi The Gab1 protein is a docking site for multiple proteins involved in signaling by the B cell antigen receptor
    R J Ingham
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 273:30630-7. 1998
    ..Far Western analysis showed that the SH2 domains of Shc, SHP-2, and the p85 subunit of phosphatidylinositol 3-kinase could bind directly to tyrosine-phosphorylated Gab1 isolated from ..
  55. ncbi Infection of human immunodeficiency virus and intracellular viral Tat protein exert a pro-survival effect in a human microglial cell line
    Pauline Chugh
    Department of Microbiology and Immunology, School of Medicine, University of Rochester Medical Center, 601 Elmwood Avenue, Box 672, Rochester, NY 14742, USA
    J Mol Biol 366:67-81. 2007
    ..We propose that the expression of Tat may enable HIV-1 infected microglia to survive throughout the course of infection, leading to persistent HIV-1 production and infection in the central nervous system...
  56. pmc Phosphatidylinositol 3-kinase regulates human immunodeficiency virus type 1 replication following viral entry in primary CD4+ T lymphocytes and macrophages
    Fleur Francois
    Division of Infectious Diseases, Mt Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    J Virol 77:2539-49. 2003
    ..This type of host-virus interaction has implications for anti-HIV therapeutics that target cellular signaling machinery...
  57. ncbi The structure of a human p110alpha/p85alpha complex elucidates the effects of oncogenic PI3Kalpha mutations
    Chuan Hsiang Huang
    Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Science 318:1744-8. 2007
    ..codes for p110alpha, the catalytic subunit of a phosphatidylinositol 3-kinase, isoform alpha (PI3Kalpha, p110alpha/p85). Here, we report a 3...
  58. ncbi Binding of HIV-1 to its receptor induces tyrosine phosphorylation of several CD4-associated proteins, including the phosphatidylinositol 3-kinase
    G Briand
    Centre de Recherche en Infectiologie, Centre Hospitalier Universitaire de Quebec, Canada
    Virology 228:171-9. 1997
    ..permitted the identification of two of these proteins, p56lck and phosphatidylinositol 3-kinase (PI 3-kinase) p85 alpha. No concomitant variation in the level of these two CD4-associated proteins was observed after HIV-1-induced CD4 ..
  59. ncbi Peptidoglycan induces nuclear factor-kappaB activation and cyclooxygenase-2 expression via Ras, Raf-1, and ERK in RAW 264.7 macrophages
    Bing Chang Chen
    School of Respiratory Therapy, Taipei Medical University, 250 Wu Hsing Street, Taipei 110, Taiwan
    J Biol Chem 279:20889-97. 2004
    ..Our data demonstrate for the first time that PGN activates the Ras/Raf-1/ERK pathway, which in turn initiates IKKalpha/beta and NF-kappaB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages...
  60. pmc Phosphotyrosine-dependent interaction of SHC and insulin receptor substrate 1 with the NPEY motif of the insulin receptor via a novel non-SH2 domain
    T A Gustafson
    Department of Physiology, University of Maryland School of Medicine, Baltimore 21201, USA
    Mol Cell Biol 15:2500-8. 1995
    ..The SAIN domain appears to represent a novel motif which is able to interact with autophosphorylated receptors such as the IR...
  61. ncbi A multifunctional docking site mediates signaling and transformation by the hepatocyte growth factor/scatter factor receptor family
    C Ponzetto
    Department of Biomedical Sciences and Oncology, University of Torino Medical School, Italy
    Cell 77:261-71. 1994
    ..The same bidentate motif is conserved in the evolutionarily related receptors Sea and Ron, suggesting that in all members of the HGF/SF receptor family, signal transduction is channeled through a multifunctional binding site...
  62. ncbi 90-kDa ribosomal S6 kinase is a direct target for the nuclear fibroblast growth factor receptor 1 (FGFR1): role in FGFR1 signaling
    Yafang Hu
    Department of Pathology and Anatomical Sciences, State University of New York, Buffalo, New York 142214, USA
    J Biol Chem 279:29325-35. 2004
    ..Thus, active FGFR1 kinase regulates the functions of nuclear RSK1. The interaction of nuclear FGFR1 with pluripotent RSK1 offers a new mechanism through which FGFR1 may control fundamental cellular processes...
  63. pmc 1-Phosphatidylinositol 3-kinase activity is required for insulin-stimulated glucose transport but not for RAS activation in CHO cells
    K Hara
    Second Department of Internal Medicine, Kobe University School of Medicine, Japan
    Proc Natl Acad Sci U S A 91:7415-9. 1994
    ..In this study, the stable overexpression in a Chinese hamster ovary (CHO) cell line of a mutant p85 alpha (delta p85) protein, which lacks a binding site for p110, disrupted the complex formation between IRS-1 and the ..
  64. ncbi Identification of a common amino acid polymorphism in the p85alpha regulatory subunit of phosphatidylinositol 3-kinase: effects on glucose disappearance constant, glucose effectiveness, and the insulin sensitivity index
    T Hansen
    Steno Diabetes Center and Hagedorn Research Institute, Glostrup University Hospital, Copenhagen, Denmark
    Diabetes 46:494-501. 1997
    ..In itself, the gene variant does not confer an increased risk of diabetes...
  65. pmc Characterization of the Met326Ile variant of phosphatidylinositol 3-kinase p85alpha
    Katrine Almind
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 99:2124-8. 2002
    ..Thus, the Met-326Ile variant of p85alpha is functional for intracellular signaling and adipocyte differentiation but has small alterations in protein expression and activity that could play a role in modifying insulin action...
  66. ncbi TGF-beta 1 modulates EGF-stimulated phosphatidylinositol 3-kinase activity in human airway smooth muscle cells
    V P Krymskaya
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    Am J Physiol 273:L1220-7. 1997
    ....
  67. pmc SH2 domains of the p85 alpha subunit of phosphatidylinositol 3-kinase regulate binding to growth factor receptors
    C J McGlade
    Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Mol Cell Biol 12:991-7. 1992
    ..The p85 alpha regulatory subunit of phosphatidylinositol (PI) 3-kinase, which associates with several receptor protein-..
  68. pmc A novel positive feedback loop mediated by the docking protein Gab1 and phosphatidylinositol 3-kinase in epidermal growth factor receptor signaling
    G A Rodrigues
    Department of Pharmacology and Skirball Institute, New York University Medical Center, New York, New York 10016, USA
    Mol Cell Biol 20:1448-59. 2000
    ..A mutant of Gab1 unable to bind the p85 subunit of PI-3 kinase is defective in potentiating EGFR signaling, confirming a role for PI-3 kinase as a ..
  69. ncbi HIV-1 gp120-mediated apoptosis of T cells is regulated by the membrane tyrosine phosphatase CD45
    Appakkudal R Anand
    Division of Experimental Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 281:12289-99. 2006
    ..These results suggest a novel CD45-mediated mechanism for the HIV envelope-induced apoptosis of T cells...
  70. ncbi Inhibition of tyrosine kinase activation blocks the down-regulation of CXC chemokine receptor 4 by HIV-1 gp120 in CD4+ T cells
    S B Su
    Laboratory of Molecular Immunoregulation, Division of Basic Sciences, SAIC Frederick National Cancer Institute, MD 21702, USA
    J Immunol 162:7128-32. 1999
    ..Thus, a CD4-associated signaling molecule(s) including p56lck is activated by gp120 and is required for the down-regulation of CXCR4...
  71. ncbi HIV-1 Tat promotes monocyte chemoattractant protein-1 secretion followed by transmigration of monocytes
    I W Park
    Division of Experimental Medicine and Hematology Oncology, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, Boston, MA 02115, USA
    Blood 97:352-8. 2001
    ..These data indicate that the HIV-1 Tat protein may act as a protocytokine by causing the release of MCP-1 from the endothelial monolayer, and thereby facilitating monocyte transmigration into tissues via a PKC signaling pathway...
  72. ncbi Regulation of the phosphatidylinositol 3-kinase, Akt/protein kinase B, FRAP/mammalian target of rapamycin, and ribosomal S6 kinase 1 signaling pathways by thyroid-stimulating hormone (TSH) and stimulating type TSH receptor antibodies in the thyroid gland
    Jae Mi Suh
    Laboratory of Endocrine Cell Biology, Department of Internal Medicine, Chungnam National University School of Medicine, 640 Daesadong Chungku, Taejon 301 040, Korea
    J Biol Chem 278:21960-71. 2003
    ..This pathway may play a role in the TSH/stimulating type TSH receptor antibody-mediated thyrocyte proliferation in vitro and in the response to TSH in vivo...
  73. ncbi Extracellular HIV-1 Tat protein activates phosphatidylinositol 3- and Akt/PKB kinases in CD4+ T lymphoblastoid Jurkat cells
    P Borgatti
    Institute of Human Anatomy, University of Ferrara, Italy
    Eur J Immunol 27:2805-11. 1997
    ..Taken together, these data suggest that the anti-apoptotic activity of low concentrations of Tat protein on Jurkat cells is mediated by a PI 3-kinase/Akt pathway...
  74. ncbi Stem cell factor induces phosphatidylinositol 3'-kinase-dependent Lyn/Tec/Dok-1 complex formation in hematopoietic cells
    T B van Dijk
    Institute of Hematology, Erasmus University Rotterdam, Rotterdam, The Netherlands
    Blood 96:3406-13. 2000
    ..These findings suggest that p62Dok-1 may function as an important scaffold molecule in cKit-mediated signaling...
  75. pmc ADP-ribosylation factor 1 controls the activation of the phosphatidylinositol 3-kinase pathway to regulate epidermal growth factor-dependent growth and migration of breast cancer cells
    Pierre Luc Boulay
    Department of Pharmacology, Faculty of Medicine, University of Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 283:36425-34. 2008
    ..These results uncover a novel molecular mechanism by which ARF1 regulates breast cancer cell growth and invasion during cancer progression...
  76. ncbi Activation of phosphatidylinositol 3-kinase/Akt pathway by androgen through interaction of p85alpha, androgen receptor, and Src
    Mei Sun
    Departments of Pathology and Interdisciplinary Oncology, University of South Florida College of Medicine and H Lee Moffitt Cancer Center, Tampa, Florida 33612, USA
    J Biol Chem 278:42992-3000. 2003
    ....
  77. ncbi Dominance of ErbB-1 heterodimers in lung epithelial cells overexpressing ErbB-2. Both ErbB-1 and ErbB-2 contribute significantly to tumorigenicity
    A M Fernandes
    Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, MD, USA
    Am J Respir Cell Mol Biol 21:701-9. 1999
    ..This work further suggests that ErbB-3 and -4 species may also contribute to tumorigenic conversion and that their expression levels may be increased by signaling initiated by TGF-alpha...
  78. pmc Intracellular movement of green fluorescent protein-tagged phosphatidylinositol 3-kinase in response to growth factor receptor signaling
    H Gillham
    Receptor Biology Laboratory, Imperial Cancer Research Fund Molecular Oncology Unit, Imperial College School of Medicine, Hammersmith Hospital, London W12 ONN, United Kingdom
    J Cell Biol 146:869-80. 1999
    ..Here we show using a green fluorescent protein-tagged p85 subunit that phosphatidylinositol 3-kinase is distributed throughout the cytoplasm and is localized to focal ..
  79. ncbi Gab-family adapter proteins act downstream of cytokine and growth factor receptors and T- and B-cell antigen receptors
    K Nishida
    Division of Molecular Oncology, Biomedical Research Center, Osaka University Medical School, Osaka, Japan
    Blood 93:1809-16. 1999
    ..Overexpression of Gab2 enhanced the gp130 or Src-related kinases-mediated ERK2 activation as that of Gab1 did. These data indicate that Gab-family molecules act as adapters for transmitting various signals...
  80. ncbi HIV-1 Nef control of cell signalling molecules: multiple strategies to promote virus replication
    Alison L Greenway
    Macfarlane Burnet Institute for Medical Research and Public Health, Cnr Commercial and Punt Roads, Melbourne, Victoria 3004, Australia
    J Biosci 28:323-35. 2003
    ....
  81. ncbi Natural variants of human p85 alpha phosphoinositide 3-kinase in severe insulin resistance: a novel variant with impaired insulin-stimulated lipid kinase activity
    K C Baynes
    Department of Medicine, University of Cambridge, UK
    Diabetologia 43:321-31. 2000
    ..Phosphoinositide 3-kinase (PI 3K) plays a central part in the mediation of insulin-stimulated glucose disposal. No genetic studies of this enzyme in human syndromes of severe insulin resistance have been previously reported...
  82. ncbi Non-SH2 domains within insulin receptor substrate-1 and SHC mediate their phosphotyrosine-dependent interaction with the NPEY motif of the insulin-like growth factor I receptor
    A Craparo
    Department of Physiology, University of Maryland School of Medicine, Baltimore 21201, USA
    J Biol Chem 270:15639-43. 1995
    ..In this study we demonstrate that IRS-1, SHC, and the p85 subunit of phosphatidylinositol 3-kinase interact directly and specifically with the IGFIR...
  83. ncbi Ca(2+)-evoked serotonin secretion by parafollicular cells: roles in signal transduction of phosphatidylinositol 3'-kinase, and the gamma and zeta isoforms of protein kinase C
    K Liu
    Division of Neuroscience, New York State Psychiatric Institute, New York, New York 10032, USA
    J Neurosci 20:1365-73. 2000
    ..PF cells were found to express regulatory (p85) and catalytic (p110alpha and p110beta) subunits of phosphatidylinositol 3'-kinase (PI3'-kinase)...
  84. ncbi Involvement of Raf-1 kinase and protein kinase C zeta in insulin-like growth factor I-induced brown adipocyte mitogenic signaling cascades: inhibition by cyclic adenosine 3',5'-monophosphate
    A M Valverde
    , Facultad de Farmacia, Universidad Complutense, Madrid, Spain
    Endocrinology 137:3832-41. 1996
    ..5-fold, whereas the tyrosine-phosphorylated IGF-I receptor was not directly associated with the p85 subunit of the phosphatidylinositol 3-kinase...
  85. ncbi Growth hormone and prolactin stimulate tyrosine phosphorylation of insulin receptor substrate-1, -2, and -3, their association with p85 phosphatidylinositol 3-kinase (PI3-kinase), and concomitantly PI3-kinase activation via JAK2 kinase
    T Yamauchi
    Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113, Japan
    J Biol Chem 273:15719-26. 1998
    ..In addition, the major tyrosine-phosphorylated protein in anti-p85 phosphatidylinositol 3-kinase (PI3-kinase) immunoprecipitate from liver of wild-type mice was IRS-1, and IRS-2 in ..
  86. ncbi Solution structure of the C-terminal SH2 domain of the p85 alpha regulatory subunit of phosphoinositide 3-kinase
    G Siegal
    Ludwig Institute for Cancer Research, London, UK
    J Mol Biol 276:461-78. 1998
    ..The solution structure of the uncomplexed C-terminal SH2 (C-SH2) domain of the p85 alpha subunit of PI 3-kinase has been determined by means of multinuclear, double and triple-resonance NMR experiments ..
  87. ncbi Syk, a protein-tyrosine kinase, suppresses the cell motility and nuclear factor kappa B-mediated secretion of urokinase type plasminogen activator by inhibiting the phosphatidylinositol 3'-kinase activity in breast cancer cells
    Ganapati H Mahabeleshwar
    National Centre for Cell Science NCCS, NCCS Complex, Pune 411 007, India
    J Biol Chem 278:6209-21. 2003
    ..and stimulated the interaction between the inhibitor of nuclear factor kappa B alpha (I kappa B alpha) and the p85 alpha domain of PI 3'-kinase through tyrosine phosphorylation of the I kappa B alpha, which ultimately resulted in ..
  88. ncbi A p85 subunit-independent p110alpha PI 3-kinase colocalizes with p70 S6 kinase on actin stress fibers and regulates thrombin-stimulated stress fiber formation in swiss 3T3 cells
    S O Johanson
    John Curtin School of Medical Research, Australian National University, Canberra, 2600, Australia
    Exp Cell Res 248:223-33. 1999
    ..In contrast, PI 3-kinase p110gamma colocalized with microtubules. The PI 3-kinase p85 subunit, known to be capable of association with p110alpha, was present in a predominantly golgi-like distribution ..
  89. ncbi Insulin-like growth factor-1 (IGF-1) receptor-insulin receptor substrate complexes in the uterus. Altered signaling response to estradiol in the IGF-1(m/m) mouse
    R G Richards
    Hormones and Cancer Group, Laboratory of Molecular Carcinogenesis, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    J Biol Chem 273:11962-9. 1998
    ..Immunoprecipitates of uterine Tyr(P) IRS-1 contained both p85, the regulatory subunit of phosphatidylinositol (PI) 3-kinase, and PI 3-kinase catalytic activity...
  90. ncbi PI3-kinase is involved in mitogenic signaling by the oncogenic receptor tyrosine kinase Xiphophorus melanoma receptor kinase in fish melanoma
    C Wellbrock
    Biocenter Theodor Boveri Institut, University of Wurzburg, Wurzburg, Germany
    Exp Cell Res 251:340-9. 1999
    ..Constitutive binding of the p85 adapter subunit of PI3-kinase to the Xmrk receptor was detected in PSM melanoma cells...
  91. ncbi Phosphatidylinositol-3 kinase activity is regulated by BCR/ABL and is required for the growth of Philadelphia chromosome-positive cells
    T Skorski
    Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Blood 86:726-36. 1995
    ..We show that the p85 alpha subunit of PI-3 kinase associates with BCR/ABL and that transient expression of BCR/ABL in fibroblasts and down-..
  92. pmc A functional 14-3-3zeta-independent association of PI3-kinase with glycoprotein Ib alpha, the major ligand-binding subunit of the platelet glycoprotein Ib-IX-V complex
    Fi Tjen Mu
    Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Australia
    Blood 111:4580-7. 2008
    ..Pull-down experiments involving glutathione S-transferase (GST)-PI3-kinase/p85-subunit and GST-14-3-3zeta indicated that both proteins interacted with contiguous GPIb alpha sequences 580 to 590/..
  93. pmc Tyr721 regulates specific binding of the CSF-1 receptor kinase insert to PI 3'-kinase SH2 domains: a model for SH2-mediated receptor-target interactions
    M Reedijk
    Department of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ont, Canada
    EMBO J 11:1365-72. 1992
    ..The p85 alpha subunit of PI 3'-kinase contains two Src homology (SH)2 domains, which are implicated in the interactions of ..
  94. ncbi Role of protein phosphatase 2A in calcium-dependent chloride secretion by human colonic epithelial cells
    Jimmy Y C Chow
    Division of Gastroenterology, 0063 University Center 303, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0063, USA
    Am J Physiol Cell Physiol 292:C452-9. 2007
    ..Although both EGF and carbachol cause tyrosine phosphorylation of p85 of PI 3-kinase, only EGF activates the enzyme...
  95. ncbi Endogenous angiotensin II suppresses insulin signaling in vascular smooth muscle cells from spontaneously hypertensive rats
    N Fukuda
    Second Department of Internal Medicine, Nihon University School of Medicine, Tokyo 173 8610, Japan
    J Hypertens 19:1651-8. 2001
    ..We have demonstrated that VSMC from spontaneously hypertensive rats (SHR) produce Ang II in a homogeneous culture...
  96. ncbi Insulin-like growth factors require phosphatidylinositol 3-kinase to signal myogenesis: dominant negative p85 expression blocks differentiation of L6E9 muscle cells
    P Kaliman
    Departament de Bioquimica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Spain
    Mol Endocrinol 12:66-77. 1998
    ..involvement of PI 3-kinase in IGF-induced myogenesis by overexpressing in L6E9 myoblasts a dominant negative p85 PI 3-kinase-regulatory subunit (L6E9-delta p85)...
  97. pmc Receptor-mediated endocytosis of albumin by kidney proximal tubule cells is regulated by phosphatidylinositide 3-kinase
    N J Brunskill
    Department of Cell Physiology and Pharmacology, Leicester University Medical School, Leicester LE1 9HN, United Kingdom
    J Clin Invest 101:2140-50. 1998
    ..Recycling rates were unaffected. We transfected OK cells with either a wild-type p85 subunit of PI 3-kinase, or a dominant negative form of the p85 subunit (Deltap85) using the LacSwitch expression ..
  98. ncbi Formation of c-Cbl.phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism
    D Hartley
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 271:21939-43. 1996
    ..studies from our laboratory have shown that c-Cbl is the predominant tyrosine-phosphorylated protein bound to the p85 subunit of phosphatidylinositol (PI) 3-kinase on T lymphocyte and B lymphocyte activation...
  99. doi Functional association of phosphoinositide-3-kinase with platelet glycoprotein Ibalpha, the major ligand-binding subunit of the glycoprotein Ib-IX-V complex
    F T Mu
    Australian Centre for Blood Diseases, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Victoria, Australia
    J Thromb Haemost 8:324-30. 2010
    ..subunit contains binding sites for filamin (residues 561-572, critically Phe568/Trp570), 14-3-3zeta (involving phosphorylation sites Ser587/590 and Ser609), and the phosphoinositide-3-kinase (PI3-kinase) regulatory subunit, p85.
  100. ncbi A novel T608R missense mutation in insulin receptor substrate-1 identified in a subject with type 2 diabetes impairs metabolic insulin signaling
    Diana L Esposito
    Department of Oncology and Neurosciences, Section of Molecular Pathology, University Gabriele D Annunzio, 66013 Chieti, Italy
    J Clin Endocrinol Metab 88:1468-75. 2003
    ..immunoprecipitated from transfected cells treated with or without insulin was subjected to immunoblotting for the p85 regulatory subunit of PI 3-kinase as well as a PI 3-kinase assay...
  101. ncbi Positive and negative roles of p85 alpha and p85 beta regulatory subunits of phosphoinositide 3-kinase in insulin signaling
    Kohjiro Ueki
    Research Division, Joslin Diabetes Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 278:48453-66. 2003
    ..To explore the physiological roles of two major regulatory isoforms, p85 alpha and p85 beta, we have established brown adipose cell lines with disruption of the Pik3r1 or Pik3r2 gene...

Research Grants64

  1. Nutritional Effects on Insulin Signaling and Action
    Marc Andre Cornier; Fiscal Year: 2007
    ..PI 3-kinase consists of a regulatory subunit, p85, and a catalytic subunit, p110. Normally, the regulatory subunit exists in excess to the catalytic one...
  2. PTEN TUMOR SUPPRESSOR AND SIGNAL TRANSDUCTION
    Ramon Parsons; Fiscal Year: 2005
    ..The p85 and p110 subunits of PI-3 kinase and AKT are potent oncogenes...
  3. Myocardial ischemic activation of the Akt pathway
    STEPHEN ARMSTRONG; Fiscal Year: 2007
    ..bDG) assembles a complex of signaling molecules that allows an association with and activation of the p85 subunit of PI-3K, initiating Akt phosphorylation...
  4. PI 3 KINASE ISOFORMS AND INSULIN ACTION
    C Kahn; Fiscal Year: 2003
    ..subunits of PI 3-kinase, six of which are the result of three different alternative splicing events of the p85 gene. Both PI 3-kinase activity and the alternative splicing are regulated in animal models of diabetes...
  5. Novel Function of PI 3-Kinase in Calcium Regulation
    Ching Shih Chen; Fiscal Year: 2004
    ..a molecular genetic approach in which Jurkat T cells are stably transfected with a dominant-negative form of the p85 regulatory subunit to suppress the activity of endogenous PI 3-kinase...
  6. ANG II AND IGF-1 INTERACTION IN CARDIOVASCULAR TISSUE
    James Sowers; Fiscal Year: 2003
    ..In order to interrupt the PI3-kinase pathway, they will transfect a dominant-negative p85 construct using adenovirus and FuGENE methodologies in VSMC and neonatal cardiomyocytes, respectively...
  7. IFNARI SIGNALING THROUGH STAT3, PI 3 KINASE
    LAWRENCE PFEFFER; Fiscal Year: 2002
    ..To be determined is if STAT3 activation (specifically via the docking domain in STAT3 for the p85 subunit of PI-3 kinase) is required for IFNalpha-induced: PI-3 kinase activation, PKC activation, ERK2 activation, ..
  8. PI 3-Kinase Isoforms in Insulin Action
    C Kahn; Fiscal Year: 2007
    ..one between PI 3-kinase regulatory subunits and activation of the stress kinases JNK and p38, which may link the p85 subunit to serine phosphorylation of IRS proteins in insulin resistant states; and a second between the PI 3-kinase ..
  9. PI 3-Kinase Isoforms in Insulin Action
    C Kahn; Fiscal Year: 2009
    ..one between PI 3-kinase regulatory subunits and activation of the stress kinases JNK and p38, which may link the p85 subunit to serine phosphorylation of IRS proteins in insulin resistant states; and a second between the PI 3-kinase ..
  10. PI 3-Kinase Isoforms in Insulin Action
    C Ronald Kahn; Fiscal Year: 2010
    ..one between PI 3-kinase regulatory subunits and activation of the stress kinases JNK and p38, which may link the p85 subunit to serine phosphorylation of IRS proteins in insulin resistant states;and a second between the PI 3-kinase ..
  11. p85/p110 PI3 Kinase-Structure, function and Physiology
    Jonathan M Backer; Fiscal Year: 2010
    ..The Class IA PI 3-kinase is a dimer containing distinct regulatory (p85) and catalytic (p110) subunits, both of which are frequently mutated in human cancer...
  12. PI 3-Kinase Isoforms and Insulin Action
    C Kahn; Fiscal Year: 2006
    ..subunits independent of PI 3-kinase activity, especially the JNK and p38 MAPK pathways, and the link between p85 and lipid phosphatase PTEN, and their differential ability to generate and stabilize PIP3. 2...
  13. ADIPOCYTE PROTEIN SECRETION AND INSULIN ACTION
    Harvey Lodish; Fiscal Year: 2007
    ..Overexpression of wild type proteins will be assessed for p85 and p110 subunits of PI3-kinase, Akt, PKCepsilon, PKCzeta, PIP kinase, dynamin l and 2, caveolins 1, 2, and 3, and ..
  14. Selective Insulin Resistance- Diabetic Cardiovascular Diseases
    George L King; Fiscal Year: 2010
    ..2) Identify the role of phospho-Thr86 of p85/PI3K in causing selective insulin resistance in EC and correlate the changes of phospho-Thr86/p85 with vascular ..
  15. INSULIN RECEPTOR SIGNALING IN THE RETINA
    RAJU RAJALA; Fiscal Year: 2002
    ..Recently, we have reported that the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K) interacts directly with the insulin receptor-beta ..
  16. NUTRITIONAL AND BIOCHEMICAL/GENETIC MARKERS OF CANCER
    Jing Ma; Fiscal Year: 2007
    ..the following proteins: the GH1, insulin receptor substrate (IRS)-1, IRS-2, posphatidylinositol 3'- kinase (PI3K)-p85 subunit, peroxisome proliferator-activated receptor (PPARgamma, a nuclear hormone receptor that plays a key role in ..
  17. Activating PTPN11 and c-kit Mutations in Myeloproliferative Disorder
    Rebecca Chan; Fiscal Year: 2007
    ..and proteomic experiments will identify a full range of functions that are controlled by Shp-2 and c-kit via p85 subunits of class IA PI3K and will provide novel targets for molecular therapies in the treatment of JMML and ..
  18. Akt-regulated pathways in platelet function
    Donna S Woulfe; Fiscal Year: 2011
    ..Our preliminary studies show that arrestins form agonist-dependent complexes with p85-PI3K subunits in human platelets...
  19. Modulation of Leukocyte Adhesion by P-selectin/PSGL-1 Signaling
    Jian Guo Geng; Fiscal Year: 2009
    ..Engagement of PSGL-1 by P-selectin phosphorylated the Y552PPM motif of Naf1 for recruiting p85 subunit of phosphatidylinositol-3 kinase (PI3K) and triggering the signaling cascade that culminated in activation ..
  20. Modulation of Leukocyte Adhesion by P-selectin/PSGL-1 Signaling
    Jian Guo Geng; Fiscal Year: 2010
    ..Engagement of PSGL-1 by P-selectin phosphorylated the Y552PPM motif of Naf1 for recruiting p85 subunit of phosphatidylinositol-3 kinase (PI3K) and triggering the signaling cascade that culminated in activation ..