OGT

Summary

Gene Symbol: OGT
Description: O-linked N-acetylglucosamine (GlcNAc) transferase
Alias: HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-GlcNAc transferase p110 subunit, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), O-linked N-acetylglucosamine transferase 110 kDa subunit, UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase, uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
Species: human
Products:     OGT

Top Publications

  1. Lazarus M, Nam Y, Jiang J, Sliz P, Walker S. Structure of human O-GlcNAc transferase and its complex with a peptide substrate. Nature. 2011;469:564-7 pubmed publisher
    The essential mammalian enzyme O-linked ?-N-acetylglucosamine transferase (O-GlcNAc transferase, here OGT) couples metabolic status to the regulation of a wide variety of cellular signalling pathways by acting as a nutrient sensor...
  2. Capotosti F, Guernier S, Lammers F, Waridel P, Cai Y, Jin J, et al. O-GlcNAc transferase catalyzes site-specific proteolysis of HCF-1. Cell. 2011;144:376-88 pubmed publisher
    ..We show here that the HCF-1(PRO) repeat is recognized by O-linked ?-N-acetylglucosamine transferase (OGT), which both O-GlcNAcylates the HCF-1(N) subunit and directly cleaves the HCF-1(PRO) repeat...
  3. Macauley M, Whitworth G, Debowski A, Chin D, Vocadlo D. O-GlcNAcase uses substrate-assisted catalysis: kinetic analysis and development of highly selective mechanism-inspired inhibitors. J Biol Chem. 2005;280:25313-22 pubmed
  4. Schimpl M, Zheng X, Borodkin V, Blair D, Ferenbach A, Schüttelkopf A, et al. O-GlcNAc transferase invokes nucleotide sugar pyrophosphate participation in catalysis. Nat Chem Biol. 2012;8:969-74 pubmed publisher
    ..of intracellular proteins in metazoa, catalyzed by O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) using unknown mechanisms of transfer and substrate recognition...
  5. Sakabe K, Hart G. O-GlcNAc transferase regulates mitotic chromatin dynamics. J Biol Chem. 2010;285:34460-8 pubmed publisher
    ..Here, we report that N-acetylglucosamine (O-GlcNAc) transferase (OGT), the enzyme catalyzing the addition of O-GlcNAc moieties to nuclear and cytoplasmic proteins at serine and ..
  6. Krzeslak A, Forma E, Bernaciak M, Romanowicz H, Brys M. Gene expression of O-GlcNAc cycling enzymes in human breast cancers. Clin Exp Med. 2012;12:61-5 pubmed publisher
    ..Cycling of O-GlcNAc residues on intracellular proteins is controlled by two enzymes, O-GlcNAc transferease (OGT), which catalyses the addition of O-GlcNAc residues and nucleocytoplasmic ?-N-acetylglucosaminidase (O-GlcNAcase; ..
  7. Iyer S, Akimoto Y, Hart G. Identification and cloning of a novel family of coiled-coil domain proteins that interact with O-GlcNAc transferase. J Biol Chem. 2003;278:5399-409 pubmed
    ..and cytosolic proteins by beta-O-linked N-acetylglucosamine (O-GlcNAc) is catalyzed by O-GlcNAc transferase (OGT)...
  8. Iyer S, Hart G. Roles of the tetratricopeptide repeat domain in O-GlcNAc transferase targeting and protein substrate specificity. J Biol Chem. 2003;278:24608-16 pubmed
    ..and cytosolic proteins by beta-O-linked N-acetylglucosamine (O-GlcNAc) is catalyzed by O-GlcNAc-transferase (OGT)...
  9. Deplus R, Delatte B, Schwinn M, Defrance M, Méndez J, Murphy N, et al. TET2 and TET3 regulate GlcNAcylation and H3K4 methylation through OGT and SET1/COMPASS. EMBO J. 2013;32:645-55 pubmed publisher
    ..Here, we show a direct interaction of TET2 and TET3 with O-GlcNAc transferase (OGT). OGT does not appear to influence hmC activity, rather TET2 and TET3 promote OGT activity...

More Information

Publications88

  1. Zhang Q, Liu X, Gao W, Li P, Hou J, Li J, et al. Differential regulation of the ten-eleven translocation (TET) family of dioxygenases by O-linked ?-N-acetylglucosamine transferase (OGT). J Biol Chem. 2014;289:5986-96 pubmed publisher
    ..Here we report that the O-linked ?-GlcNAc (O-GlcNAc) transferase (OGT) is not only a major TET3-interacting protein but also regulates TET3 subcellular localization and enzymatic ..
  2. Caldwell S, Jackson S, Shahriari K, Lynch T, Sethi G, Walker S, et al. Nutrient sensor O-GlcNAc transferase regulates breast cancer tumorigenesis through targeting of the oncogenic transcription factor FoxM1. Oncogene. 2010;29:2831-42 pubmed publisher
    ..cancer cells upregulate the HBP, including increased O-GlcNAcation and elevated expression of O-GlcNAc transferase (OGT), which is the enzyme catalyzing the addition of O-GlcNAc to proteins...
  3. Lazarus B, Love D, Hanover J. Recombinant O-GlcNAc transferase isoforms: identification of O-GlcNAcase, yes tyrosine kinase, and tau as isoform-specific substrates. Glycobiology. 2006;16:415-21 pubmed
    O-linked N-acetylglucosaminyltransferase (OGT) catalyzes the transfer of O-linked GlcNAc to serine or threonine residues of a variety of substrate proteins, including nuclear pore proteins, transcription factors, and proteins implicated ..
  4. Lazarus B, Roos M, Hanover J. Mutational analysis of the catalytic domain of O-linked N-acetylglucosaminyl transferase. J Biol Chem. 2005;280:35537-44 pubmed
    O-Linked N-acetylglucosaminyltransferase (OGT) catalyzes the transfer of O-linked GlcNAc to serine/threonine residues of a variety of target proteins, many of which have been implicated in such diseases as diabetes and neurodegeneration...
  5. Hanover J, Yu S, Lubas W, Shin S, Ragano Caracciola M, Kochran J, et al. Mitochondrial and nucleocytoplasmic isoforms of O-linked GlcNAc transferase encoded by a single mammalian gene. Arch Biochem Biophys. 2003;409:287-97 pubmed
    O-Linked N-acetylglucosamine (GlcNAc) transferase (OGT) mediates a novel hexosamine-dependent signal transduction pathway. Yet, little is known about the regulation of ogt gene expression in mammals...
  6. Lubas W, Hanover J. Functional expression of O-linked GlcNAc transferase. Domain structure and substrate specificity. J Biol Chem. 2000;275:10983-8 pubmed
    O-GlcNAc transferase (OGT) modifies nuclear pore proteins and transcription factors. In Arabidopsis, the OGT homolog participates in the gibberellin signaling pathway...
  7. Chen Q, Chen Y, Bian C, Fujiki R, Yu X. TET2 promotes histone O-GlcNAcylation during gene transcription. Nature. 2013;493:561-4 pubmed publisher
    ..of TET proteins, and find that TET2 and TET3 associate with O-linked ?-N-acetylglucosamine (O-GlcNAc) transferase (OGT), an enzyme that by itself catalyses the addition of O-GlcNAc onto serine and threonine residues (O-GlcNAcylation) ..
  8. Kreppel L, Blomberg M, Hart G. Dynamic glycosylation of nuclear and cytosolic proteins. Cloning and characterization of a unique O-GlcNAc transferase with multiple tetratricopeptide repeats. J Biol Chem. 1997;272:9308-15 pubmed
    ..We have cloned and characterized a novel gene for an O-GlcNAc transferase (OGT) that shares no sequence homology or structural similarities with other glycosyltransferases...
  9. Pathak S, Borodkin V, Albarbarawi O, Campbell D, Ibrahim A, van Aalten D. O-GlcNAcylation of TAB1 modulates TAK1-mediated cytokine release. EMBO J. 2012;31:1394-404 pubmed publisher
    ..This is one of the first examples of a single O-GlcNAc site on a signalling protein modulating a key innate immunity signalling pathway. ..
  10. Shen D, Gloster T, Yuzwa S, Vocadlo D. Insights into O-linked N-acetylglucosamine ([0-9]O-GlcNAc) processing and dynamics through kinetic analysis of O-GlcNAc transferase and O-GlcNAcase activity on protein substrates. J Biol Chem. 2012;287:15395-408 pubmed publisher
    ..O-GlcNAc) levels are modulated by two enzymes: uridine diphosphate-N-acetyl-D-glucosamine:polypeptidyltransferase (OGT) and O-GlcNAcase (OGA)...
  11. Vella P, Scelfo A, Jammula S, Chiacchiera F, Williams K, Cuomo A, et al. Tet proteins connect the O-linked N-acetylglucosamine transferase Ogt to chromatin in embryonic stem cells. Mol Cell. 2013;49:645-56 pubmed publisher
    O-linked N-acetylglucosamine (O-GlcNAc) transferase (Ogt) activity is essential for embryonic stem cell (ESC) viability and mouse development...
  12. Wang Z, Udeshi N, Slawson C, Compton P, Sakabe K, Cheung W, et al. Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis. Sci Signal. 2010;3:ra2 pubmed publisher
    ..Overexpression of the enzyme that adds O-GlcNAc to target proteins, O-GlcNAc transferase (OGT), perturbs cytokinesis and promotes polyploidy, but the molecular targets of OGT that are important for its cell ..
  13. Yang X, Ongusaha P, Miles P, Havstad J, Zhang F, So W, et al. Phosphoinositide signalling links O-GlcNAc transferase to insulin resistance. Nature. 2008;451:964-9 pubmed publisher
    ..Here we show that O-GlcNAc transferase (OGT) harbours a previously unrecognized type of phosphoinositide-binding domain...
  14. Shafi R, Iyer S, Ellies L, O Donnell N, Marek K, Chui D, et al. The O-GlcNAc transferase gene resides on the X chromosome and is essential for embryonic stem cell viability and mouse ontogeny. Proc Natl Acad Sci U S A. 2000;97:5735-9 pubmed
    ..by the addition of N-acetylglucosamine (GlcNAc) to serine and threonine is catalyzed by the O-GlcNAc transferase (OGT)...
  15. Wrabl J, Grishin N. Homology between O-linked GlcNAc transferases and proteins of the glycogen phosphorylase superfamily. J Mol Biol. 2001;314:365-74 pubmed
    ..However, no structural information about the human OGT exists, except for the identification of tetratricopeptide repeats (TPR) at the N terminus...
  16. McClain D, Lubas W, Cooksey R, Hazel M, Parker G, Love D, et al. Altered glycan-dependent signaling induces insulin resistance and hyperleptinemia. Proc Natl Acad Sci U S A. 2002;99:10695-9 pubmed
    ..These data support the proposal that O-linked GlcNAc transferase participates in a hexosamine-dependent signaling pathway that is linked to insulin resistance and leptin production. ..
  17. Love D, Kochan J, Cathey R, Shin S, Hanover J, Kochran J. Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase. J Cell Sci. 2003;116:647-54 pubmed
    O-linked GlcNAc transferase (OGT) mediates a novel glycan-dependent signaling pathway, but the intracellular targeting of OGT is poorly understood...
  18. Wysocka J, Myers M, Laherty C, Eisenman R, Herr W. Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1. Genes Dev. 2003;17:896-911 pubmed
    ..These results suggest that HCF-1 can broadly regulate transcription, both positively and negatively, through selective modulation of chromatin structure. ..
  19. Lubas W, Frank D, Krause M, Hanover J. O-Linked GlcNAc transferase is a conserved nucleocytoplasmic protein containing tetratricopeptide repeats. J Biol Chem. 1997;272:9316-24 pubmed
    ..Based on its substrate specificity and molecular features, we propose that O-linked GlcNAc transferase is part of a glucose-responsive pathway previously implicated in the pathogenesis of diabetes mellitus. ..
  20. Martinez Fleites C, Macauley M, He Y, Shen D, Vocadlo D, Davies G. Structure of an O-GlcNAc transferase homolog provides insight into intracellular glycosylation. Nat Struct Mol Biol. 2008;15:764-5 pubmed publisher
    ..UDP-GlcNAc:polypeptidyl transferase (OGT) catalyzes O-GlcNAc addition...
  21. Lazarus M, Jiang J, Gloster T, Zandberg W, Whitworth G, Vocadlo D, et al. Structural snapshots of the reaction coordinate for O-GlcNAc transferase. Nat Chem Biol. 2012;8:966-8 pubmed publisher
    ..The structures reveal key features of the mechanism and show that substrate participation is important during catalysis. ..
  22. Lynch T, Ferrer C, Jackson S, Shahriari K, Vosseller K, Reginato M. Critical role of O-Linked ?-N-acetylglucosamine transferase in prostate cancer invasion, angiogenesis, and metastasis. J Biol Chem. 2012;287:11070-81 pubmed publisher
    ..Here, we show that O-GlcNAc transferase (OGT) is overexpressed in prostate cancer compared with normal prostate epithelium and that OGT protein and O-GlcNAc ..
  23. Yang X, Zhang F, Kudlow J. Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: coupling protein O-GlcNAcylation to transcriptional repression. Cell. 2002;110:69-80 pubmed
    ..Here, we show that O-GlcNAc transferase (OGT), the enzyme that catalyzes this posttranslational modification, interacts with a histone deacetylase complex by ..
  24. Trinca G, Hagan C. O-GlcNAcylation in women's cancers: breast, endometrial and ovarian. J Bioenerg Biomembr. 2018;50:199-204 pubmed publisher
    ..Herein, we review the impact of altered O-GlcNAcylation in breast, ovarian and uterine cancers. ..
  25. Myers S, Peddada S, Chatterjee N, Friedrich T, Tomoda K, Krings G, et al. SOX2 O-GlcNAcylation alters its protein-protein interactions and genomic occupancy to modulate gene expression in pluripotent cells. elife. 2016;5:e10647 pubmed publisher
    ..These findings show that SOX2 activity is modulated by O-GlcNAc, and provide a novel regulatory mechanism for this crucial pluripotency transcription factor. ..
  26. Souza Silva L, Alves Lopes R, Silva Miguez J, Dela Justina V, Neves K, Mestriner F, et al. Glycosylation with O-Linked ?-N-acetylglucosamine (O-GlcNAc) induces vascular dysfunction via production of superoxide anion / reactive oxygen species. Can J Physiol Pharmacol. 2017;: pubmed publisher
    ..In conclusion, O-GlcNAc levels reduce endothelium-dependent relaxation by overproduction of •O2- via activation of NADPH oxidase. This may represent an additional mechanism by which augmented O-GlcNAc levels impair vascular function. ..
  27. Eustice M, Bond M, Hanover J. O-GlcNAc cycling and the regulation of nucleocytoplasmic dynamics. Biochem Soc Trans. 2017;45:427-436 pubmed publisher
    ..Furthermore, O-GlcNAc plays a role in maintaining the structural integrity of the nuclear pore and regulating its function as the gatekeeper of nucleocytoplasmic trafficking. ..
  28. Leturcq M, Lefebvre T, Vercoutter Edouart A. O-GlcNAcylation and chromatin remodeling in mammals: an up-to-date overview. Biochem Soc Trans. 2017;45:323-338 pubmed publisher
    ..Over the past decade, the nutrient-sensor enzyme O-GlcNAc transferase (OGT) has emerged as a modulator of chromatin remodeling...
  29. Slawinska A, Plowiec A, Siwek M, Jaroszewski M, Bednarczyk M. Long-Term Transcriptomic Effects of Prebiotics and Synbiotics Delivered In Ovo in Broiler Chickens. PLoS ONE. 2016;11:e0168899 pubmed publisher
    ..expression (PAPOLA, RPL27A, RPLP1, and RPS29) from P1 and P2 in transport (BEST4, SLC9A3, and SLC13A2), metabolism (OGT, ALPP, CA4, and CA7), signaling (FGG, G3BP2, UBB, G3BP2, CACNA1G, and ATP6V0A4), and immune responses (MSMB, LGALS3,..
  30. Vaiana C, Kurćon T, Mahal L. MicroRNA-424 Predicts a Role for β-1,4 Branched Glycosylation in Cell Cycle Progression. J Biol Chem. 2016;291:1529-37 pubmed publisher
    ..Herein we identify MGAT4A, OGT, and GALNT13 as targets of miR-424...
  31. Oosterman J, Belsham D. Glucose Alters Per2 Rhythmicity Independent of AMPK, Whereas AMPK Inhibitor Compound C Causes Profound Repression of Clock Genes and AgRP in mHypoE-37 Hypothalamic Neurons. PLoS ONE. 2016;11:e0146969 pubmed publisher
    ..peptide (AgRP), carnitine palmitoyltransferase 1C (Cpt1c), and O-linked N-acetylglucosamine transferase (Ogt) were measured...
  32. Yang Y, Yin X, Yang H, Xu Y. Histone demethylase LSD2 acts as an E3 ubiquitin ligase and inhibits cancer cell growth through promoting proteasomal degradation of OGT. Mol Cell. 2015;58:47-59 pubmed publisher
    ..LSD2 directly ubiquitylates and promotes proteasome-dependent degradation of O-GlcNAc transferase (OGT), and inhibits A549 lung cancer cell growth in a manner dependent on its E3 ligase activity, but not demethylase ..
  33. Zhang N, Chen X. Potential role of O-GlcNAcylation and involvement of PI3K/Akt1 pathway in the expression of oncogenic phenotypes of gastric cancer cells in vitro. Biotechnol Appl Biochem. 2016;63:841-851 pubmed publisher
    ..O-GlcNAcylation is dynamically regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). Increasing evidence suggests that O-GlcNAcylation is involved in a variety of human cancers...
  34. Li S, Zhu H, Wang J, Wang X, Li X, Ma C, et al. Comparative analysis of Cu (I)-catalyzed alkyne-azide cycloaddition (CuAAC) and strain-promoted alkyne-azide cycloaddition (SPAAC) in O-GlcNAc proteomics. Electrophoresis. 2016;37:1431-6 pubmed publisher
  35. Harosh Davidovich S, Khalaila I. O-GlcNAcylation affects β-catenin and E-cadherin expression, cell motility and tumorigenicity of colorectal cancer. Exp Cell Res. 2018;364:42-49 pubmed publisher
    ..Stable silenced cell lines with adenoviral OGA or adenoviral O-GlcNAc transferase (OGT) were established...
  36. Gatta E, Lefebvre T, Gaetani S, Dos Santos M, Marrocco J, Mir A, et al. Evidence for an imbalance between tau O-GlcNAcylation and phosphorylation in the hippocampus of a mouse model of Alzheimer's disease. Pharmacol Res. 2016;105:186-97 pubmed publisher
    ..These data demonstrate that an imbalance between tau phosphorylation and O-GlcNAcylation exists in AD mice, and strengthens the hypothesis that O-GlcNAcylation might be targeted by disease modifying drugs in AD. ..
  37. Kakade P, Budnar S, Kalraiya R, Vaidya M. Functional Implications of O-GlcNAcylation-dependent Phosphorylation at a Proximal Site on Keratin 18. J Biol Chem. 2016;291:12003-13 pubmed publisher
    ..Here we report a novel, positive interplay between O-GlcNAcylation and phosphorylation at adjacent sites on K18 to regulate its fundamental properties. ..
  38. Hu J, Chen R, Jia P, Fang Y, Liu T, Song N, et al. Augmented O-GlcNAc signaling via glucosamine attenuates oxidative stress and apoptosis following contrast-induced acute kidney injury in rats. Free Radic Biol Med. 2017;103:121-132 pubmed publisher
    ..Collectively, the results demonstrate that glucosamine induces renoprotection against CI-AKI through augmented O-GlcNAc and activation of PI3K/Akt signaling, making it a promising strategy for preventing CI-AKI. ..
  39. Dassanayaka S, Brainard R, Watson L, Long B, Brittian K, Demartino A, et al. Cardiomyocyte Ogt limits ventricular dysfunction in mice following pressure overload without affecting hypertrophy. Basic Res Cardiol. 2017;112:23 pubmed publisher
    ..To test the role of OGT in the heart, we subjected cardiomyocyte-specific, inducibly deficient Ogt (i-cmOgt -/-) mice and Ogt ..
  40. Very N, Steenackers A, Dubuquoy C, Vermuse J, Dubuquoy L, Lefebvre T, et al. Cross regulation between mTOR signaling and O-GlcNAcylation. J Bioenerg Biomembr. 2018;50:213-222 pubmed publisher
    ..O-GlcNAc transferase (OGT) is the enzyme which transfers the N-acetylglucosamine (O-GlcNAc) residue onto target proteins...
  41. Ripberger E, Linnebacher M, Schwitalle Y, Gebert J, von Knebel Doeberitz M. Identification of an HLA-A0201-restricted CTL epitope generated by a tumor-specific frameshift mutation in a coding microsatellite of the OGT gene. J Clin Immunol. 2003;23:415-23 pubmed
    ..a frameshift mutation in the coding T(10) microsatellite of the O-linked N-acetylglucosamine transferase gene (OGT) occuring in up to 41% of microsatellite unstable colorectal cancers...
  42. Ferrer C, Lu T, Bacigalupa Z, Katsetos C, Sinclair D, Reginato M. O-GlcNAcylation regulates breast cancer metastasis via SIRT1 modulation of FOXM1 pathway. Oncogene. 2017;36:559-569 pubmed publisher
    ..The nutrient sensor O-linked-?-N-acetylglucosamine (O-GlcNAc) transferase (OGT) modifies intracellular proteins with N-acetylglucosamine...
  43. Kim D, Cutler J, Na C, Reckel S, Renuse S, Madugundu A, et al. BioSITe - a method for direct detection and quantitation of site-specific biotinylation. J Proteome Res. 2017;: pubmed publisher
    ..Overall, we anticipate that BioSITe will replace the conventional methods in studies where detection of biotinylation sites is important...
  44. Chen J, Liu X, Lü F, Liu X, Ru Y, Ren Y, et al. Transcription factor Nrf1 is negatively regulated by its O-GlcNAcylation status. FEBS Lett. 2015;589:2347-58 pubmed publisher
    O-Linked N-acetylglucosamine transferase (OGT) was identified as an Nrf1-interacting protein. Herein, we show that Nrf1 enables interaction with OGT and their co-immunoprecipitates are O-GlcNAcylated by the enzyme...
  45. Rodriguez A, Yu S, Li B, Zegzouti H, Kohler J. Enhanced transfer of a photocross-linking N-acetylglucosamine (GlcNAc) analog by an O-GlcNAc transferase mutant with converted substrate specificity. J Biol Chem. 2015;290:22638-48 pubmed publisher
    ..O-GlcNAc modification is catalyzed by the O-GlcNAc transferase (OGT), which transfers N-acetylglucosamine (GlcNAc) from the nucleotide sugar donor UDP-GlcNAc to serine or threonine ..
  46. Slawson C, Lakshmanan T, Knapp S, Hart G. A mitotic GlcNAcylation/phosphorylation signaling complex alters the posttranslational state of the cytoskeletal protein vimentin. Mol Biol Cell. 2008;19:4130-40 pubmed publisher
    ..Here, we demonstrate that the enzymes responsible for O-GlcNAc cycling, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) are in a transient complex at M phase with the mitotic kinase Aurora B and protein ..
  47. Cheng Y, Li H, Li J, Li J, Gao Y, Liu B. O-GlcNAcylation enhances anaplastic thyroid carcinoma malignancy. Oncol Lett. 2016;12:572-578 pubmed
    ..O-GlcNAcylation is dynamically regulated by O-GlcNAc transferase (OGT) and O-GlcNAc hydrolase (OGA)...
  48. Cole R, Hart G. Glycosylation sites flank phosphorylation sites on synapsin I: O-linked N-acetylglucosamine residues are localized within domains mediating synapsin I interactions. J Neurochem. 1999;73:418-28 pubmed
    ..We conclude that O-GlcNAcylation likely plays a more direct role in synapsin I interactions than simply modulating the protein's phosphorylation. ..
  49. Wang X, Feng Z, Wang X, Yang L, Han S, Cao K, et al. O-GlcNAcase deficiency suppresses skeletal myogenesis and insulin sensitivity in mice through the modulation of mitochondrial homeostasis. Diabetologia. 2016;59:1287-96 pubmed publisher
    ..In this manner, OGA appears to play a key role in myogenesis and the development of muscle insulin resistance. ..
  50. Shin S, Love D, Hanover J. Elevated O-GlcNAc-dependent signaling through inducible mOGT expression selectively triggers apoptosis. Amino Acids. 2011;40:885-93 pubmed publisher
    O-linked N-acetylglucosamine transferase (OGT) catalyzes O-GlcNAc addition to numerous cellular proteins including transcription and nuclear pore complexes and plays a key role in cellular signaling...
  51. Taylor R, Parker G, Hazel M, Soesanto Y, Fuller W, Yazzie M, et al. Glucose deprivation stimulates O-GlcNAc modification of proteins through up-regulation of O-linked N-acetylglucosaminyltransferase. J Biol Chem. 2008;283:6050-7 pubmed publisher
    ..Rather, the mRNA for nucleocytoplasmic O-linked N-acetylglucosaminyltransferase (OGT) increases 3.4-fold within 6 h of glucose deprivation (p = 0.006). Within 12 h, OGT protein increases 1...
  52. Griffin M, Jensen E, Mason D, Jenkins C, Stone S, Peters E, et al. Comprehensive mapping of O-GlcNAc modification sites using a chemically cleavable tag. Mol Biosyst. 2016;12:1756-9 pubmed publisher
    ..unambiguously identifying several established O-GlcNAc sites on the proteins ?-crystallin and O-GlcNAc transferase (OGT), as well as discovering new, previously unreported sites on OGT...
  53. Inoue Y, Moriwaki K, Ueda Y, Takeuchi T, Higuchi K, Asahi M. Elevated O-GlcNAcylation stabilizes FOXM1 by its reduced degradation through GSK-3β inactivation in a human gastric carcinoma cell line, MKN45 cells. Biochem Biophys Res Commun. 2018;495:1681-1687 pubmed publisher
  54. Dong X, Tang H, Hess K, Abbruzzese J, Li D. Glucose metabolism gene polymorphisms and clinical outcome in pancreatic cancer. Cancer. 2011;117:480-91 pubmed publisher
    ..004 and .007. The authors demonstrated that glucose metabolism gene polymorphisms affect clinical outcome in pancreatic cancer. These observations support a role of abnormal glucose metabolism in pancreatic carcinogenesis. ..
  55. Golks A, Tran T, Goetschy J, Guerini D. Requirement for O-linked N-acetylglucosaminyltransferase in lymphocytes activation. EMBO J. 2007;26:4368-79 pubmed
    ..proteins with O-linked beta-N-acetylglucosamine (O-GlcNAc) by the O-linked N-acetylglucosaminyltransferase (OGT) is a regulatory post-translational modification that is responsive to various stimuli...
  56. Burén S, Gomes A, Teijeiro A, Fawal M, Yilmaz M, Tummala K, et al. Regulation of OGT by URI in Response to Glucose Confers c-MYC-Dependent Survival Mechanisms. Cancer Cell. 2016;30:290-307 pubmed publisher
    ..We demonstrate here that glucose maintains a functional complex between the co-chaperone URI, PP1γ, and OGT, the enzyme catalyzing O-GlcNAcylation...
  57. Liu W, Wang H, Xue X, Xia J, Liu J, Qi Z, et al. OGT-related mitochondrial motility is associated with sex differences and exercise effects in depression induced by prenatal exposure to glucocorticoids. J Affect Disord. 2018;226:203-215 pubmed publisher
    ..As an X-linked gene in rodents and in humans, O-GlcNAc transferase (OGT) may provide a novel perspective for the sex differences in depression...
  58. Okuyama R, Marshall S. UDP-N-acetylglucosaminyl transferase (OGT) in brain tissue: temperature sensitivity and subcellular distribution of cytosolic and nuclear enzyme. J Neurochem. 2003;86:1271-80 pubmed
    In brain tissue, UDP-N-acetylglucosaminyl transferase (OGT) is known to catalyze the addition of a single N-acetylglucosamine moiety (GlcNAc) onto two proteins linked to the etiology of neurodegenerative disease--beta-amyloid associated ..
  59. Groussaud D, Khair M, Tollenaere A, Waast L, Kuo M, Mangeney M, et al. Hijacking of the O-GlcNAcZYME complex by the HTLV-1 Tax oncoprotein facilitates viral transcription. PLoS Pathog. 2017;13:e1006518 pubmed publisher
    ..O-GlcNAc) on Serine or Threonine residues, a process controlled by two enzymes: O-GlcNAc transferase (OGT), which transfers O-GlcNAc on proteins, and O-GlcNAcase (OGA), which removes it...
  60. Wu D, Cai Y, Jin J. Potential coordination role between O-GlcNAcylation and epigenetics. Protein Cell. 2017;8:713-723 pubmed publisher
    ..O-GlcNAc modification (O-GlcNAcylation) are controlled by O-linked ?-N-acetylglucosamine (O-GlcNAc) transferase (OGT) and the glycoside hydrolase O-GlcNAcase (OGA) in cells...
  61. Bauer C, Göbel K, Nagaraj N, Colantuoni C, Wang M, Müller U, et al. Phosphorylation of TET proteins is regulated via O-GlcNAcylation by the O-linked N-acetylglucosamine transferase (OGT). J Biol Chem. 2015;290:4801-12 pubmed publisher
    ..Our data suggest strong cross-talk between the modification sites that could allow rapid adaption of TET protein localization, activity, or targeting due to changing environmental conditions as well as in response to external stimuli. ..
  62. Cox N, Unlu G, Bisnett B, Meister T, Condon B, Luo P, et al. Dynamic Glycosylation Governs the Vertebrate COPII Protein Trafficking Pathway. Biochemistry. 2018;57:91-107 pubmed publisher
    ..Our results indicate that O-GlcNAc is a conserved and critical regulatory modification in the vertebrate COPII-dependent trafficking pathway. ..
  63. Wen T, Hou K, Li Z, Li L, Yu H, Liu Y, et al. Silencing β-linked N-acetylglucosamine transferase induces apoptosis in human gastric cancer cells through PUMA and caspase-3 pathways. Oncol Rep. 2015;34:3140-6 pubmed publisher
    linked N-acetylglucosamine (GlcNAc) is a monosaccharide that is catalyzed by O-GlcNAcylation transferase (OGT) to bind serine or threonine hydroxyl moieties of numerous nuclear and cytoplasmic proteins...
  64. Ortiz Meoz R, Merbl Y, Kirschner M, Walker S. Microarray discovery of new OGT substrates: the medulloblastoma oncogene OTX2 is O-GlcNAcylated. J Am Chem Soc. 2014;136:4845-8 pubmed publisher
    O-GlcNAc transferase (OGT) is a serine/threonine glycosyltransferase that is essential for development and continues to be critically important throughout life. Understanding OGT's complex biology requires identifying its substrates...
  65. Lazarus M, Jiang J, Kapuria V, Bhuiyan T, Janetzko J, Zandberg W, et al. HCF-1 is cleaved in the active site of O-GlcNAc transferase. Science. 2013;342:1235-9 pubmed publisher
    ..is cleaved by the nutrient-responsive glycosyltransferase, O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT)...
  66. Daou S, Mashtalir N, Hammond Martel I, Pak H, Yu H, Sui G, et al. Crosstalk between O-GlcNAcylation and proteolytic cleavage regulates the host cell factor-1 maturation pathway. Proc Natl Acad Sci U S A. 2011;108:2747-52 pubmed publisher
    ..We demonstrate that a large proportion of the signaling enzyme O-linked-N-acetylglucosaminyl transferase (OGT) is complexed with HCF-1 and this interaction is essential for HCF-1 cleavage...
  67. Widiati E, Nurhaeni N, Gayatri D. Medical-Device Related Pressure Injuries to Children in the Intensive Care Unit. Compr Child Adolesc Nurs. 2017;40:69-77 pubmed publisher
    ..Health devices that caused pressure injuries on respondents were: ETT (13%), OGT (12%), NGT (11%), and SpO2 probe (6%)...
  68. Medina L, Grove K, Haltiwanger R. SV40 large T antigen is modified with O-linked N-acetylglucosamine but not with other forms of glycosylation. Glycobiology. 1998;8:383-91 pubmed
    ..These two serines fall within a typical O-linked N-acetylglucosamine glycosylation site (PSS) and are also known to be phosphorylated. Thus, it is likely that competition between phosphorylation and glycosylation occurs at this site. ..
  69. Constable S, Lim J, Vaidyanathan K, Wells L. O-GlcNAc transferase regulates transcriptional activity of human Oct4. Glycobiology. 2017;27:927-937 pubmed publisher
    ..Addition of this modification, by the enzyme O-linked N-acetylglucosamine transferase (OGT), is dynamic and inducible. One major class of proteins modified by O-GlcNAc is transcription factors...
  70. Groves J, Maduka A, O Meally R, Cole R, Zachara N. Fatty acid synthase inhibits the O-GlcNAcase during oxidative stress. J Biol Chem. 2017;292:6493-6511 pubmed publisher
    ..The mechanisms by which the O-GlcNAc transferase (OGT) and the O-GlcNAcase (OGA), the enzymes that add and remove O-GlcNAc, respectively, are regulated ..
  71. Ho S, Wang K, Whisenhunt T, Huang P, Zhu X, Kudlow J, et al. O-GlcNAcylation enhances FOXO4 transcriptional regulation in response to stress. FEBS Lett. 2010;584:49-54 pubmed publisher
    ..We show that hydrogen peroxide treatment of HEK293 cells increases FOXO4 association with OGT, the enzyme that adds O-GlcNAc to proteins, causing FOXO4 O-GlcNAcylation and enhanced transcriptional activity ..
  72. Lu L, Fan D, Hu C, Worth M, Ma Z, Jiang J. Distributive O-GlcNAcylation on the Highly Repetitive C-Terminal Domain of RNA Polymerase II. Biochemistry. 2016;55:1149-58 pubmed publisher
    ..a minimum of 20 heptad repeats of the CTD and more than half of the N-terminal domain of O-GlcNAc transferase (OGT)...
  73. Ishimura E, Nakagawa T, Moriwaki K, Hirano S, Matsumori Y, Asahi M. Augmented O-GlcNAcylation of AMP-activated kinase promotes the proliferation of LoVo cells, a colon cancer cell line. Cancer Sci. 2017;: pubmed publisher
    ..Collectively, the higher colon cancer risk of diabetic patients could be due to O-GlcNAcylation-mediated AMPK inactivation and subsequent activation of mTOR...
  74. Kim G, Cao L, Reece E, Zhao Z. Impact of protein O-GlcNAcylation on neural tube malformation in diabetic embryopathy. Sci Rep. 2017;7:11107 pubmed publisher
    ..GlcNAc can be added to proteins by O-GlcNAc transferase (OGT) to regulate protein activity...
  75. Pælestik K, Jespersen N, Jensen R, Johnsen J, Bøtker H, Kristiansen S. Effects of hypoglycemia on myocardial susceptibility to ischemia-reperfusion injury and preconditioning in hearts from rats with and without type 2 diabetes. Cardiovasc Diabetol. 2017;16:148 pubmed publisher
    ..In parallel with IPC induced cardioprotection, MGU and O-GlcNAc levels increase suggesting that increased MGU and O-GlcNAc levels are involved in the mechanisms of IPC. ..
  76. Stewart L, Khan A, Wang K, Pizarro D, Pati S, Buckingham S, et al. Acute Increases in Protein O-GlcNAcylation Dampen Epileptiform Activity in Hippocampus. J Neurosci. 2017;37:8207-8215 pubmed publisher
    ..Together, our findings support the conclusion that protein O-GlcNAcylation is a regulator of neuronal excitability, and it represents a promising target for further research on seizure disorder therapeutics. ..
  77. Taylor R, Geisler T, Chambers J, McClain D. Up-regulation of O-GlcNAc transferase with glucose deprivation in HepG2 cells is mediated by decreased hexosamine pathway flux. J Biol Chem. 2009;284:3425-32 pubmed publisher
    ..We conclude that deglycosylation of proteins within the first few hours of glucose deprivation promotes ncOGT induction. These findings suggest a novel negative feedback regulatory loop for OGT and O-GlcNAc regulation.
  78. Zhu G, Tao T, Zhang D, Liu X, Qiu H, Han L, et al. O-GlcNAcylation of histone deacetylases 1 in hepatocellular carcinoma promotes cancer progression. Glycobiology. 2016;26:820-833 pubmed publisher
    ..Previous studies have indicated that O-GlcNAc transferase (OGT) and histone deacetylase-1 (HDAC1) play important roles in the pathogenesis of HCC...
  79. Howerton C, Morgan C, Fischer D, Bale T. O-GlcNAc transferase (OGT) as a placental biomarker of maternal stress and reprogramming of CNS gene transcription in development. Proc Natl Acad Sci U S A. 2013;110:5169-74 pubmed publisher
    ..We identifed O-linked-N-acetylglucosamine (O-GlcNAc) transferase (OGT), an X-linked gene important in regulating proteins involved in chromatin remodeling, as fitting these criteria...