OCLN

Summary

Gene Symbol: OCLN
Description: occludin
Alias: BLCPMG, tight junction protein occludin
Species: human

Top Publications

  1. pmc Alteration of tight junction proteins is an early event in psoriasis: putative involvement of proinflammatory cytokines
    Nina Kirschner
    Department of Dermatology and Venerology, University Hospital Hamburg Eppendorf, Martinistrasse 52, Hamburg, Germany
    Am J Pathol 175:1095-106. 2009
  2. ncbi Epidermal tight junctions: ZO-1 and occludin are expressed in mature, developing, and affected skin and in vitro differentiating keratinocytes
    K Pummi
    Department of Medical Biochemistry, University of Turku, Finland
    J Invest Dermatol 117:1050-8. 2001
  3. pmc Tight junction-associated MARVEL proteins marveld3, tricellulin, and occludin have distinct but overlapping functions
    David R Raleigh
    Department of Pathology, The University of Chicago, Chicago, IL 60637, USA
    Mol Biol Cell 21:1200-13. 2010
  4. pmc The tight junction protein complex undergoes rapid and continuous molecular remodeling at steady state
    Le Shen
    Department of Pathology, The University of Chicago, Chicago, IL 60637, USA
    J Cell Biol 181:683-95. 2008
  5. pmc ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin
    J Haskins
    Department of Cell Biology and Anatomy, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Cell Biol 141:199-208. 1998
  6. pmc Species-specific regions of occludin required by hepatitis C virus for cell entry
    Maria L Michta
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA
    J Virol 84:11696-708. 2010
  7. doi Redox-sensitivity of the dimerization of occludin
    J K Walter
    Leibniz Institut für Molekulare Pharmakologie Berlin, FMP, Robert Rossle Str 10, 13125, Berlin Buch, Germany
    Cell Mol Life Sci 66:3655-62. 2009
  8. doi Different expression of occludin and ZO-1 in primary and metastatic liver tumors
    Erika Orbán
    2nd Department of Pathology, Semmelweis University, Budapest, Hungary
    Pathol Oncol Res 14:299-306. 2008
  9. doi Differential phosphorylation of occludin and tricellulin by CK2 and CK1
    Max J Dörfel
    Department of Laboratory Medicine and Pathobiochemistry, Charite Universitatsmedizin Berlin, Berlin, Germany
    Ann N Y Acad Sci 1165:69-73. 2009
  10. pmc Tight junction proteins claudin-1 and occludin control hepatitis C virus entry and are downregulated during infection to prevent superinfection
    Shufeng Liu
    Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pennsylvania, USA
    J Virol 83:2011-4. 2009

Research Grants

  1. Blood brain barrier immune compromise in NeuroAIDS
    GEORGETTE KANMOGNE; Fiscal Year: 2009
  2. NeuroAIDS in Cameroon: Molecular determinants
    GEORGETTE KANMOGNE; Fiscal Year: 2007
  3. HIV-1 gp120-induced Endothelial Cell Dysfunction
    GEORGETTE KANMOGNE; Fiscal Year: 2007
  4. Blood brain barrier immune compromise in NeuroAIDS
    Georgette D Kanmogne; Fiscal Year: 2010
  5. Leukocyte Migration in Wounded Cornea
    Alan Burns; Fiscal Year: 2007
  6. Intestinal Epithelial Tight Junction Structure-Function
    Asma Nusrat; Fiscal Year: 2005
  7. Intestinal Epithelial Tight Junction Structure-Function
    Asma Nusrat; Fiscal Year: 2007
  8. Estrogen and Aging effects on Transvaginal Transport
    George Gorodeski; Fiscal Year: 2007
  9. REGULATORY PATHWAYS AND ROLE OF VPF/VEGF IN RENAL CANCER
    Debabrata Mukhopadhyay; Fiscal Year: 2007
  10. Distinct Pathways of VPF/VEGF Receptors
    Debabrata Mukhopadhyay; Fiscal Year: 2007

Scientific Experts

Detail Information

Publications79

  1. pmc Alteration of tight junction proteins is an early event in psoriasis: putative involvement of proinflammatory cytokines
    Nina Kirschner
    Department of Dermatology and Venerology, University Hospital Hamburg Eppendorf, Martinistrasse 52, Hamburg, Germany
    Am J Pathol 175:1095-106. 2009
    ..Our data indicate that cytokines are involved in alterations of TJ proteins observed in psoriasis...
  2. ncbi Epidermal tight junctions: ZO-1 and occludin are expressed in mature, developing, and affected skin and in vitro differentiating keratinocytes
    K Pummi
    Department of Medical Biochemistry, University of Turku, Finland
    J Invest Dermatol 117:1050-8. 2001
    ..We speculate that the epidermal barrier, isolating the human body from the external environment, is in part formed by tight junctions of stratum granulosum...
  3. pmc Tight junction-associated MARVEL proteins marveld3, tricellulin, and occludin have distinct but overlapping functions
    David R Raleigh
    Department of Pathology, The University of Chicago, Chicago, IL 60637, USA
    Mol Biol Cell 21:1200-13. 2010
    ..The data further suggest that these proteins are best considered as a group with both redundant and unique contributions to epithelial function and tight junction regulation...
  4. pmc The tight junction protein complex undergoes rapid and continuous molecular remodeling at steady state
    Le Shen
    Department of Pathology, The University of Chicago, Chicago, IL 60637, USA
    J Cell Biol 181:683-95. 2008
    ..These data demonstrate that the tight junction undergoes constant remodeling and suggest that this dynamic behavior may contribute to tight junction assembly and regulation...
  5. pmc ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin
    J Haskins
    Department of Cell Biology and Anatomy, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Cell Biol 141:199-208. 1998
    ..In vitro affinity analyses demonstrated that recombinant 130-kD protein directly interacts with ZO-1 and the cytoplasmic domain of occludin, but not with ZO-2. We propose that this protein be named ZO-3...
  6. pmc Species-specific regions of occludin required by hepatitis C virus for cell entry
    Maria L Michta
    Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA
    J Virol 84:11696-708. 2010
    ..It has been previously determined that the tight junction protein occludin (OCLN) is essential for HCV host cell entry and that human OCLN is more efficient than the mouse ..
  7. doi Redox-sensitivity of the dimerization of occludin
    J K Walter
    Leibniz Institut für Molekulare Pharmakologie Berlin, FMP, Robert Rossle Str 10, 13125, Berlin Buch, Germany
    Cell Mol Life Sci 66:3655-62. 2009
    ..It is concluded that the redox-dependent dimerization of occludin may play a regulatory role in the tight junction assembly under physiological and pathological conditions...
  8. doi Different expression of occludin and ZO-1 in primary and metastatic liver tumors
    Erika Orbán
    2nd Department of Pathology, Semmelweis University, Budapest, Hungary
    Pathol Oncol Res 14:299-306. 2008
    ..HCCs and metastases are characterized by markedly different protein expression pattern of occludin and ZO-1, which phenomenon might be attributed to the different histogenesis of these tumors...
  9. doi Differential phosphorylation of occludin and tricellulin by CK2 and CK1
    Max J Dörfel
    Department of Laboratory Medicine and Pathobiochemistry, Charite Universitatsmedizin Berlin, Berlin, Germany
    Ann N Y Acad Sci 1165:69-73. 2009
    ..This chapter compares occludin and tricellulin phosphorylation by the serine/threonine kinases CK2 and CK1...
  10. pmc Tight junction proteins claudin-1 and occludin control hepatitis C virus entry and are downregulated during infection to prevent superinfection
    Shufeng Liu
    Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pennsylvania, USA
    J Virol 83:2011-4. 2009
    ..The altered TJ protein expression may contribute to the morphological and functional changes observed in HCV-infected hepatocytes...
  11. pmc Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins
    M Itoh
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 147:1351-63. 1999
    ..In good agreement, PDZ1 and PDZ2 domains of ZO-1/ZO-2/ZO-3 were also recruited to claudin-based TJs, when introduced into cultured epithelial cells. The possible molecular architecture of TJ plaque structures is discussed...
  12. ncbi Occludin phosphorylation: identification of an occludin kinase in brain and cell extracts as CK2
    Caroline Smales
    Eisai London Research Laboratories Ltd, Bernard Katz Building, University College London, Gower Street, London, WC1E 6BT, UK
    FEBS Lett 545:161-6. 2003
    ..CK2 is therefore a candidate kinase for regulation of occludin phosphorylation in vivo...
  13. ncbi HIV-1 gp120 proteins alter tight junction protein expression and brain endothelial cell permeability: implications for the pathogenesis of HIV-associated dementia
    Georgette D Kanmogne
    Department of Pharmacology, Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, Nebraska 68198 5215, USA
    J Neuropathol Exp Neurol 64:498-505. 2005
    ....
  14. doi Zonula occludens-1, occludin, and E-cadherin protein expression in biliary tract cancers
    Zsuzsanna Nemeth
    2nd Department of Pathology, Semmelweis University, Ulloi ut 93, H 1091 Budapest, Hungary
    Pathol Oncol Res 15:533-9. 2009
    ..These results were confirmed by discriminant analysis yielding clear separation of the three normal sample groups from carcinomas in the corresponding locations...
  15. pmc Identification and analysis of occludin phosphosites: a combined mass spectrometry and bioinformatics approach
    Jeffrey M Sundstrom
    Department of Cellular and Molecular Physiology, Penn State University College of Medicine, Hershey, Pennsylvania 17033, USA
    J Proteome Res 8:808-17. 2009
    ..This study demonstrates that combining MS data and bioinformatics can successfully identify novel phosphorylation sites from limiting samples...
  16. pmc Phosphorylation of Tyr-398 and Tyr-402 in occludin prevents its interaction with ZO-1 and destabilizes its assembly at the tight junctions
    Bertha C Elias
    Department of Physiology, Charles B Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    J Biol Chem 284:1559-69. 2009
    ..This study reveals a unique motif in the occludin sequence that is involved in the regulation of ZO-1 binding by reversible phosphorylation of specific Tyr residues...
  17. ncbi Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin
    M Itoh
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Biol Chem 274:5981-6. 1999
    ..These findings indicated that ZO-2 forms a complex with ZO-1/occludin or ZO-1/alpha catenin to establish TJ or AJ domains, respectively...
  18. pmc The second extracellular loop dictates Occludin-mediated HCV entry
    Shufeng Liu
    Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Virology 407:160-70. 2010
    Recent findings have implicated tight junction (TJ) protein Occludin (OCLN) as an essential factor for hepatitis C virus (HCV) to enter human hepatocytes...
  19. pmc The tight junction-associated protein occludin is required for a postbinding step in hepatitis C virus entry and infection
    Ignacio Benedicto
    Hospital Universitario de la Princesa, Madrid, Spain
    J Virol 83:8012-20. 2009
    ..In summary, we propose that occludin plays an essential role in HCV infection and probably affects late entry events. This observation may provide new insights into HCV infection and related pathogenesis...
  20. pmc Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5
    Carolyn B Coyne
    Division of Infectious Diseases, Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
    Cell Host Microbe 2:181-92. 2007
    ..Thus, CVB entry depends on occludin and occurs by a process that combines aspects of caveolar endocytosis with features characteristic of macropinocytosis...
  21. ncbi Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3
    E S Wittchen
    Department of Cell Biology, University of Alberta, Edmonton T6G 2H7, Canada
    J Biol Chem 274:35179-85. 1999
    ..ZO-3 complexes rather than a trimeric ZO-1.ZO-2.ZO-3 grouping. These studies elucidate direct binding interactions among tight junction-associated proteins, giving insight into their organization as a multimolecular structure...
  22. ncbi Use of surface plasmon resonance for real-time analysis of the interaction of ZO-1 and occludin
    A Schmidt
    Institute of Molecular Pharmacology, Robert Rossle Strasse 10, 13125 Berlin, Germany
    Biochem Biophys Res Commun 288:1194-9. 2001
    ..We demonstrate the use of SPR spectroscopy as an effective approach for characterisation of the interactions of junction proteins...
  23. ncbi Occludin expression decreases with the progression of human endometrial carcinoma
    Hirotoshi Tobioka
    Department of Pathology, Sapporo Medical University School of Medicine, Japan
    Hum Pathol 35:159-64. 2004
    ..These results suggest that the loss of tight junctions has a close relationship with structural atypia in the progression of human endometrial carcinomas and their malignant potential...
  24. pmc Occludin is required for cytokine-induced regulation of tight junction barriers
    Christina M Van Itallie
    Department of Medicine, University of North Carolina, Chapel Hill, NC 27599, USA
    J Cell Sci 123:2844-52. 2010
    ..These results demonstrate that occludin is required for cells to transduce cytokine-mediated signals that either increase the electrical barrier or decrease the large solute barrier, possibly by coordinating the functions of caveolin-1...
  25. doi A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion
    Jin Mo Gu
    Department of Biological Sciences and Seoul National University, 56 1 Shillim dong, Kwanak gu, Seoul, Korea
    FEBS J 275:3145-56. 2008
    The tight junction protein occludin participates in cell adhesion and migration and has been shown to possess antitumorigenic properties; however, the exact mechanism underlying these effects is poorly understood...
  26. ncbi Structure of the conserved cytoplasmic C-terminal domain of occludin: identification of the ZO-1 binding surface
    Yuanhe Li
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, Chicago, IL 60607, USA
    J Mol Biol 352:151-64. 2005
    ..Our results provide a model to further test the function of occludin and its binding to other proteins...
  27. ncbi The second loop of occludin is required for suppression of Raf1-induced tumor growth
    Zili Wang
    Epithelial Pathobiology Research Unit, Department of Pathology, Emory University, 615 Michael Street, Atlanta, GA 30322, USA
    Oncogene 24:4412-20. 2005
    ..These results demonstrate that the TJ protein occludin has a potent inhibitory effect on the Raf1-mediated tumorigenesis, and the second extracellular loop of occludin appears to be critical for this function...
  28. ncbi The tight junction-specific protein occludin is a functional target of the E3 ubiquitin-protein ligase itch
    Andreas Traweger
    Institute of Molecular Biology, Austrian Academy of Sciences, 5020 Salzburg, Austria
    J Biol Chem 277:10201-8. 2002
    ..In addition, we provide evidence that Itch is specifically involved in the ubiquitination of occludin in vivo, and that the degradation of occludin is sensitive to proteasome inhibition...
  29. ncbi In vitro protein complex formation with cytoskeleton-anchoring domain of occludin identified by limited proteolysis
    Bi Hung Peng
    Department of Pathology, The University of Texas Medical Branch, Galveston, Texas 77555 0750, USA
    J Biol Chem 278:49644-51. 2003
    ..These results provide a basis for development of a structural model of the ZO-binding site that can be used to investigate regulation of tight junction anchoring by intracellular signaling events...
  30. ncbi Occludin confers adhesiveness when expressed in fibroblasts
    C M Van Itallie
    Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510, USA
    J Cell Sci 110:1113-21. 1997
    ..These results suggest that the extracellular surface of occludin is directly involved in cell-cell adhesion and the ability to confer adhesiveness correlates with the ability to colocalize with its cytoplasmic binding protein, ZO-1...
  31. ncbi Occludin TM4(-): an isoform of the tight junction protein present in primates lacking the fourth transmembrane domain
    M Reza Ghassemifar
    Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK Department of Biology, University of York, PO Box 373, York YO10 5YW, UK
    J Cell Sci 115:3171-80. 2002
    The tight junction protein occludin possesses four transmembrane domains, two extracellular loops, and cytoplasmic N- and C-termini...
  32. ncbi The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton
    A S Fanning
    Department of Internal Medicine, Yale School of Medicine, New Haven, Connecticut 06510, USA
    J Biol Chem 273:29745-53. 1998
    ..These observations suggest that one functional role of ZO-1 is to organize components of the tight junction and link them to the cortical actin cytoskeleton...
  33. pmc Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues
    Y Ando-Akatsuka
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
    J Cell Biol 133:43-7. 1996
    ..Implications of these data and novel experimental options in cell biological research are discussed...
  34. pmc Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions
    M Furuse
    Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, Aichi, Japan
    J Cell Biol 127:1617-26. 1994
    ..The coincidence of the sequence necessary for the ZO-1 association with that for the TJ localization suggests that the association with underlying cytoskeletons through ZO-1 is required for occludin to be localized at TJ...
  35. pmc VEGF-mediated disruption of endothelial CLN-5 promotes blood-brain barrier breakdown
    Azeb Tadesse Argaw
    Corinne Goldsmith Dickinson Center for Multiple Sclerosis and Department of Neurology, Mount Sinai School of Medicine, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 106:1977-82. 2009
    ..Here, we identify the endothelial transmembrane tight junction proteins claudin-5 (CLN-5) and occludin (OCLN) as targets of VEGF-A action...
  36. doi A novel rearrangement of occludin causes brain calcification and renal dysfunction
    Marissa A LeBlanc
    Department of Pathology, Dalhousie University, 5850 College St, Sir Charles Tupper Medical Building, Room 11 F, Halifax, NS, Canada
    Hum Genet 132:1223-34. 2013
    ..describe the identification of a novel rearrangement in which a downstream pseudogene translocates into exon 9 of OCLN, resulting in band-like brain calcification and advanced chronic kidney disease in early childhood...
  37. doi Tight junction regulation by morphine and HIV-1 tat modulates blood-brain barrier permeability
    Supriya D Mahajan
    Division of Allergy, Immunology, and Rheumatology, Department of Medicine, Buffalo General Hospital, 301 Multi Research Bldg, 100 High Street, Buffalo, NY 14203, USA
    J Clin Immunol 28:528-41. 2008
    ..These studies may lead to the development of novel anti-HIV-1 therapeutics that target specific TJ proteins, thus preventing TJ disruption in opiate using HIV-1 patients...
  38. pmc Epithelial permeability alterations in an in vitro air-liquid interface model of allergic fungal rhinosinusitis
    Kyle A Den Beste
    Epithelial Pathobiology and Mucosal Inflammation Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30308, USA
    Int Forum Allergy Rhinol 3:19-25. 2013
    ..The aim of this study was to compare epithelial permeability and intercellular junctional protein expression among cultured primary sinonasal cells from AFRS patients vs noninflammatory controls...
  39. pmc The occludin and ZO-1 complex, defined by small angle X-ray scattering and NMR, has implications for modulating tight junction permeability
    Brian R Tash
    Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, H171, 500 University Drive, Hershey, PA 17033, USA
    Proc Natl Acad Sci U S A 109:10855-60. 2012
    ..This model has important implications for the phosphorylation-dependent regulation of the occludin:ZO-1 complex...
  40. pmc Occludin phosphorylation and ubiquitination regulate tight junction trafficking and vascular endothelial growth factor-induced permeability
    Tomoaki Murakami
    Department of Cellular and Molecular Physiology and Ophthalmology, Penn State College of Medicine, Hershey, Pennsylvania 17033, USA
    J Biol Chem 284:21036-46. 2009
    ..These data demonstrate a novel mechanism of VEGF-induced occludin phosphorylation and ubiquitination that contributes to TJ trafficking and subsequent vascular permeability...
  41. pmc Breaking down the barrier: the effects of HIV-1 on the blood-brain barrier
    Marianne Strazza
    Department of Microbiology and Immunology, and Center for Molecular Virology and Translational Neuroscience, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA 19102, USA
    Brain Res 1399:96-115. 2011
    ....
  42. doi Interleukin-18 facilitates neutrophil transmigration via myosin light chain kinase-dependent disruption of occludin, without altering epithelial permeability
    Tamia K Lapointe
    Dept of Biological Sciences, Univ of Calgary, Calgary, AB, Canada
    Am J Physiol Gastrointest Liver Physiol 302:G343-51. 2012
    ..In conclusion, our data suggest that IL-18 may potentiate inflammation in the context of IBD by facilitating neutrophil transepithelial migration via MLCK-dependent disruption of tight junctional occludin...
  43. pmc Methamphetamine alters blood brain barrier permeability via the modulation of tight junction expression: Implication for HIV-1 neuropathogenesis in the context of drug abuse
    Supriya D Mahajan
    Department of Medicine, Division of Allergy, Immunology, and Rheumatology, 301 Multi Research Building, Buffalo General Hospital, 100 High Street, Buffalo, NY 14203, USA
    Brain Res 1203:133-48. 2008
    ..An understanding of the mechanisms of BBB breakdown that lead to neurotoxicity is crucial to the development of therapeutic modalities for Meth abusing HAD patients...
  44. pmc Caveolin-1 regulates human immunodeficiency virus-1 Tat-induced alterations of tight junction protein expression via modulation of the Ras signaling
    Yu Zhong
    Molecular Neuroscience and Vascular Biology Laboratory, Department of Neurosurgery, University of Kentucky, Lexington, Kentucky 40536, USA
    J Neurosci 28:7788-96. 2008
    ..Thus, caveolin-1 may provide an effective target to protect against Tat-induced HBMEC dysfunction and the disruption of the BBB in HIV-1-infected patients...
  45. pmc Density-enhanced phosphatase 1 regulates phosphorylation of tight junction proteins and enhances barrier function of epithelial cells
    Jennifer L Sallee
    Department of Cell and Developmental Biology and Lineberger Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 284:14997-5006. 2009
    ..These data suggest that DEP-1 can modify the phosphorylation state of tight junction proteins and play a role in regulating permeability...
  46. ncbi Induction of tight junctions in human connexin 32 (hCx32)-transfected mouse hepatocytes: connexin 32 interacts with occludin
    T Kojima
    Department of Neuroscience, Albert Einstein College of Medicine, New York, NY, USA
    Biochem Biophys Res Commun 266:222-9. 1999
    ..Immunocytochemistry revealed that endogenous integral tight junction protein occludin was strongly localized and was colocalized with Cx32 at cell borders in transfectants, whereas ..
  47. pmc Impact of intra- and interspecies variation of occludin on its function as coreceptor for authentic hepatitis C virus particles
    Sandra Ciesek
    Medizinische Hochschule Hannover, Klinik fur Gastroenterologie, Hepatologie und Endokrinologie, Carl Neuberg Str 1, 30625 Hannover, Germany
    J Virol 85:7613-21. 2011
    ..The tight junction component occludin (OCLN) is essential for HCV entry into host cells, and the differences between human and murine OCLN are thought to ..
  48. doi The tight junction protein, occludin, regulates the directional migration of epithelial cells
    Dan Du
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Dev Cell 18:52-63. 2010
    ..Our data indicate that occludin is required for the leading-edge localization of polarity proteins aPKC-Par3 and PATJ and promotes cell protrusion by regulating membrane-localized activation of PI3K...
  49. ncbi Expression of human factors CD81, claudin-1, scavenger receptor, and occludin in mouse hepatocytes does not confer susceptibility to HCV entry
    Keisuke Hikosaka
    Department of Biochemistry, Hamamatsu University School of Medicine, Handa yama, Higashi ku, Hamamatsu, Shizuoka, Japan
    Biomed Res 32:143-50. 2011
    ..Our results suggest that the expression of the human factors does not confer susceptibility to HCV entry into the liver...
  50. pmc Recessive mutations in the gene encoding the tight junction protein occludin cause band-like calcification with simplified gyration and polymicrogyria
    Mary C O'Driscoll
    University of Manchester, Manchester Academic Health Science Centre, Central Manchester Foundation Trust University Hospitals, UK
    Am J Hum Genet 87:354-64. 2010
    ..We provide evidence that the tight junction protein occludin (encoded by the OCLN gene) is involved in the pathogenesis of malformations of cortical development.
  51. ncbi Loss of occludin leads to the progression of human breast cancer
    Tracey A Martin
    Metastasis and Angiogenesis Research Group, Department of Surgery, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK
    Int J Mol Med 26:723-34. 2010
    ..This loss of or aberrant expression has clear repercussions as to the importance of occludin in maintaining TJ integrity in breast tissues. Such inappropriate expression could play a part in breast cancer development...
  52. ncbi Transcriptional control of occludin expression in vascular endothelia: regulation by Sp3 and YY1
    Hadassah Sade
    Department of Life Sciences, The Open University, Milton Keynes, MK7 6AA, UK
    Biochim Biophys Acta 1789:175-84. 2009
    ..The promoter of the tight junction protein occludin exhibited strong activity in a brain endothelial cell line, hCMEC/D3 but was inactive in lung ..
  53. pmc PKC eta regulates occludin phosphorylation and epithelial tight junction integrity
    Takuya Suzuki
    Department of Physiology, University of Tennessee Health Science Center, 894 Union Avenue, Memphis, TN 38163, USA
    Proc Natl Acad Sci U S A 106:61-6. 2009
    ..These results reveal an important mechanism of epithelial TJ regulation by PKC eta...
  54. ncbi Influence of protein kinase C on transcription of the tight junction elements ZO-1 and occludin
    Frank Weiler
    Department of Medicine, Division of Gastroenterology, University of Wuerzburg, Wuerzburg, Germany
    J Cell Physiol 204:83-6. 2005
    ..Overall ZO-1 and occludin seem to be identically regulated in colonic epithelium on the transcript level...
  55. ncbi The specific fates of tight junction proteins in apoptotic epithelial cells
    Christian Bojarski
    Institute of Clinical Chemistry and Pathobiochemistry, Charite Campus Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany
    J Cell Sci 117:2097-107. 2004
    ..While the amount of claudin-2 protein was reduced similarly to occludin, ZO-1 and ZO-2, claudin-1 was either fully preserved or was even increased in apoptotic cells...
  56. ncbi Immunohistological distribution of the tight junction components ZO-1 and occludin in regenerating human epidermis
    M Malminen
    Department of Medical Biochemistry, University of Turku, Kiinamyllynkatu 4 8, 20520 Turku, Finland
    Br J Dermatol 149:255-60. 2003
    ..Tight junctions have recently been characterized in the granular cell layer of human epidermis, and the role of these junctions in the epidermal barrier is now being re-evaluated...
  57. ncbi Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail
    Junichi Ikenouchi
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Sci 116:1959-67. 2003
    ..e. the expression of adherens and tight junction adhesion molecules, respectively)...
  58. ncbi Co-localization of junction-associated proteins of the human blood--aqueous barrier: occludin, ZO-1 and F-actin
    Jeffrey Sonsino
    Department of Biosciences, New England College of Optometry, Boston, MA 02118, USA
    Exp Eye Res 74:123-9. 2002
    ....
  59. ncbi Occludin expression in microvessels of neoplastic and non-neoplastic human brain
    M C Papadopoulos
    Department of Neurosurgery, Atkinson Morley s Hospital, London SW20 0NE, UK
    Neuropathol Appl Neurobiol 27:384-95. 2001
    The tight junction protein occludin 'glues' normal, adjacent brain microvessel endothelial cells together...
  60. ncbi Expression of ZO-1 and occludin in normal human placenta and in hydatidiform moles
    D Marzioni
    Institute of Normal Human Morphology, Faculty of Medicine, University of Ancona, Via Tronto, 10 A, I 60020 Ancona, Italy
    Mol Hum Reprod 7:279-85. 2001
    ..The down-regulation and/or dysregulation of these two molecules may be related to phenotypic changes associated with epithelial cell transformation of the chorionic villi in partial and complete moles...
  61. ncbi CaCo-2 cells treated with Clostridium perfringens enterotoxin form multiple large complex species, one of which contains the tight junction protein occludin
    U Singh
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 275:18407-17. 2000
    ....
  62. ncbi Analysis of tight junctions during neutrophil transendothelial migration
    A R Burns
    Cardiovascular Sciences, Department of Medicine, Baylor College of Medicine, Houston, TX, USA
    J Cell Sci 113:45-57. 2000
    ..Collectively, these results suggest that PMN migration at tricellular corners preserves the barrier properties of the endothelium and does not involve widespread disruption of endothelial TJs...
  63. ncbi Mammalian occludin in epithelial cells: its expression and subcellular distribution
    M Saitou
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan
    Eur J Cell Biol 73:222-31. 1997
    ..Furthermore, the exclusive concentration of occludin at tight junctions in epithelial cells was confirmed by immunoreplica electron microscopy...
  64. pmc Estrogen decrease in tight junctional resistance involves matrix-metalloproteinase-7-mediated remodeling of occludin
    George I Gorodeski
    Department of Reproductive Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Endocrinology 148:218-31. 2007
    ..These mechanisms acting in concert could be important for regulation and control of estrogen modulation of paracellular permeability in vivo...
  65. ncbi Enhanced tight junction function in human breast cancer cells by antioxidant, selenium and polyunsaturated lipid
    Tracey A Martin
    Metastasis and Angiogenesis Research Group, Department of Surgery, Wales College of Medicine, Cardiff University, Cardiff, United Kingdom
    J Cell Biochem 101:155-66. 2007
    ..This combination also completely reversed the effect of 17-beta-estradiol in these cells...
  66. ncbi Tight junction proteins in gallbladder epithelium: different expression in acute acalculous and calculous cholecystitis
    Jouko J Laurila
    Department of Anesthesiology, Oulu University Hospital, P O Box 21, Oulu, Finland, 90029
    J Histochem Cytochem 55:567-73. 2007
    ....
  67. ncbi Human protein factory for converting the transcriptome into an in vitro-expressed proteome,
    Naoki Goshima
    National Institute of Advanced Industrial Science and Technology, 2 42 Aomi, Koto ku, Tokyo 135 0064, Japan
    Nat Methods 5:1011-7. 2008
    ..Our 'human protein factory' infrastructure includes the resources and expression technology for in vitro proteome research...
  68. doi Hepatitis C virus envelope components alter localization of hepatocyte tight junction-associated proteins and promote occludin retention in the endoplasmic reticulum
    Ignacio Benedicto
    Molecular Biology Unit, Hospital Universitario de la Princesa, Madrid, Spain
    Hepatology 48:1044-53. 2008
    ..Additionally, using a cell culture model of HCV infection, we observed the cytoplasmic dot-like accumulation of occludin in infected Huh7 cells...
  69. doi Glucocorticoids regulate the human occludin gene through a single imperfect palindromic glucocorticoid response element
    Nina Harke
    Institute of Anatomy and Cell Biology, University of Wurzburg, Koellikerstr 6, D 97070 Wurzburg, Germany
    Mol Cell Endocrinol 295:39-47. 2008
    ....
  70. doi Expression of occludin and claudins 1, 3, 4, and 7 in urothelial carcinoma of the upper urinary tract
    Kuniaki Nakanishi
    Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa, Japan
    Am J Clin Pathol 130:43-9. 2008
    ..Detection of claudins 1, 3, and 4 would seem to provide valuable information about the progression of UC-UUT...
  71. doi Disturbed expression of E-cadherin, beta-catenin and tight junction proteins in colon carcinoma is unrelated to growth pattern and genetic polymorphisms
    Victoria Hahn-Strömberg
    Dept of Clinical Medicine, Orebro University, Orebro, Orebro, Sweden
    APMIS 116:253-62. 2008
    ..Rather, aberrations in the function of other proteins related to the adhesive proteins could be responsible...
  72. pmc MAPK interacts with occludin and mediates EGF-induced prevention of tight junction disruption by hydrogen peroxide
    Shyamali Basuroy
    Department of Physiology, University of Tennessee Health Science Center, Memphis, TN, USA
    Biochem J 393:69-77. 2006
    ..Therefore the present study shows that ERK interacts with the C-terminal region of occludin and mediates the prevention of H2O2-induced disruption of TJs by EGF...
  73. doi Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography
    Guanghui Han
    National Chromatographic R and A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, China
    Proteomics 8:1346-61. 2008
    ..This resulted in the highly confident identification of 274 phosphorylation sites from 305 unique phosphopeptides corresponding to 168 proteins at false discovery rate (FDR) of 0.96%...
  74. pmc Cellular and molecular mechanisms of heat stress-induced up-regulation of occludin protein expression: regulatory role of heat shock factor-1
    Karol Dokladny
    Internal Medicine Gastroenterology and Hepatology, MSC10 5550, 1 University of New Mexico, Albuquerque, NM 87131 0001, USA
    Am J Pathol 172:659-70. 2008
    ....
  75. pmc A protein kinase A-dependent mechanism by which rotavirus affects the distribution and mRNA level of the functional tight junction-associated protein, occludin, in human differentiated intestinal Caco-2 cells
    Isabelle Beau
    Faculte de Pharmacie, INSERM Unit 510, Chatenay Malabry, France 92296
    J Virol 81:8579-86. 2007
    ....
  76. ncbi Tight junction proteins in keratinocytes: localization and contribution to barrier function
    Takuo Yuki
    Basic Research Laboratory, Kanebo Cosmetics Inc, Kanagawa, Japan
    Exp Dermatol 16:324-30. 2007
    ..We also found that the addition of ochratoxin A weakened the permeability barrier and the expression of claudin-4. Our findings suggest that TJ proteins contribute to the permeability barrier in epidermal keratinocytes...
  77. pmc Expression of occludin, tight-junction-associated protein, in human digestive tract
    Y Kimura
    Department of Surgery II, Osaka University Medical School, Japan
    Am J Pathol 151:45-54. 1997
    ..In addition, occludin expression can serve as a histopathological indicator for differentiation in gastrointestinal adenocarcinomas...

Research Grants60

  1. Blood brain barrier immune compromise in NeuroAIDS
    GEORGETTE KANMOGNE; Fiscal Year: 2009
    ..The work in this proposal will help us understand how HIV damages the brain endothelium and enters the brain, how to prevent viral entry into the brain, and HIV-associated dementia. ..
  2. NeuroAIDS in Cameroon: Molecular determinants
    GEORGETTE KANMOGNE; Fiscal Year: 2007
    ..It will provide knowledge on the epidemiology of neuroAIDS in Cameroon and build the capacity for future neuroAIDS research and therapeutic testing in Cameroon. ..
  3. HIV-1 gp120-induced Endothelial Cell Dysfunction
    GEORGETTE KANMOGNE; Fiscal Year: 2007
    ....
  4. Blood brain barrier immune compromise in NeuroAIDS
    Georgette D Kanmogne; Fiscal Year: 2010
    ..The work in this proposal will help us understand how HIV damages the brain endothelium and enters the brain, how to prevent viral entry into the brain, and HIV-associated dementia. ..
  5. Leukocyte Migration in Wounded Cornea
    Alan Burns; Fiscal Year: 2007
    ....
  6. Intestinal Epithelial Tight Junction Structure-Function
    Asma Nusrat; Fiscal Year: 2005
    ....
  7. Intestinal Epithelial Tight Junction Structure-Function
    Asma Nusrat; Fiscal Year: 2007
    ..Additionally, understanding the molecular composition of AJCs will also facilitate in designing molecules that can be delivered via the paracellular route. ..
  8. Estrogen and Aging effects on Transvaginal Transport
    George Gorodeski; Fiscal Year: 2007
    ....
  9. REGULATORY PATHWAYS AND ROLE OF VPF/VEGF IN RENAL CANCER
    Debabrata Mukhopadhyay; Fiscal Year: 2007
    ....
  10. Distinct Pathways of VPF/VEGF Receptors
    Debabrata Mukhopadhyay; Fiscal Year: 2007
    ..Hence, the results of these proposed studies will help identify potential angiogenic inhibitors or promoters that can be targeted and used to improve future therapies against different diseases. ..
  11. Intestinal Epithelial Tight Junction Structure-Function
    Asma Nusrat; Fiscal Year: 2010
    ..Additionally, understanding the molecular composition of AJCs will also facilitate in designing molecules that can be delivered via the paracellular route. ..
  12. REGULATORY PATHWAYS AND ROLE OF VPF/VEGF IN RENAL CANCER
    Debabrata Mukhopadhyay; Fiscal Year: 2010
    ....
  13. Targeting Pancreatic Cancer Using Peptide Chemistry: From Bench to Bedside
    Mark R Spaller; Fiscal Year: 2010
    ..The proposed study might define the mechanism of pancreatic cancer growth and progression and identify specific targets for therapeutic interventions for pancreatic adenocarcinoma where no current therapy is available. ..
  14. Enterovirus entry into polarized endothelial cells
    Carolyn B Coyne; Fiscal Year: 2010
    ..The studies in this proposal will identify cellular genes required for mediating enterovirus infection and may lead to the design of cell-type-specific antiviral targets. ..
  15. Distinct Pathways of VPF/VEGF Receptors
    Debabrata Mukhopadhyay; Fiscal Year: 2010
    ..Hence, the results of these proposed studies will help identify potential angiogenic inhibitors or promoters that can be targeted and used to improve future therapies against different diseases. ..
  16. Intestinal Mucosal Wound Resealing in IBD
    Asma Nusrat; Fiscal Year: 2010
    ....
  17. Intestinal Epithelial Tight Junction Structure-Function
    Asma Nusrat; Fiscal Year: 2009
    ..Additionally, understanding the molecular composition of AJCs will also facilitate in designing molecules that can be delivered via the paracellular route. ..
  18. REGULATORY PATHWAYS AND ROLE OF VPF/VEGF IN RENAL CANCER
    Debabrata Mukhopadhyay; Fiscal Year: 2009
    ....
  19. Intestinal Mucosal Wound Resealing in IBD
    Asma Nusrat; Fiscal Year: 2009
    ....
  20. Role of Beta Subunit of Sodium Pump in Cell Adhesion
    Ayyappan Rajasekaran; Fiscal Year: 2007
    ..abstract_text> ..
  21. Intestinal Mucosal Wound Resealing
    Asma Nusrat; Fiscal Year: 2007
    ..abstract_text> ..
  22. The Neurotransmitter dopamine and angiogenesis
    Debabrata Mukhopadhyay; Fiscal Year: 2007
    ..abstract_text> ..
  23. MUCOSAL PROTECTIVE FACTORS IN HUMAN MILK
    Radhakrishna Rao; Fiscal Year: 2003
    ..protective factors in breast milk, and provide the basis for further characterization of the mechanisms involved in mucosal protection by breast milk, and their role in preventing neonatal gastrointestinal diseases ..
  24. Neurotransmitter dopamine and angiogenesis
    Debabrata Mukhopadhyay; Fiscal Year: 2003
    ..abstract_text> ..
  25. AGING EFFECTS ON TRANSVAGINAL TRANSPORT
    George Gorodeski; Fiscal Year: 2002
    ..These data may improve our understanding of estrogen replacement to postmenopausal women. They may also provide efficient and simple modalities for birth control by targeting known mechanisms of transcervical transport. ..
  26. The Neurotransmitter dopamine and angiogenesis
    Debabrata Mukhopadhyay; Fiscal Year: 2006
    ..abstract_text> ..
  27. Distinct Pathways of VPF/VEGF Receptors
    Debabrata Mukhopadhyay; Fiscal Year: 2005
    ..Taken together these experiments are likely to identify new therapeutic targets in order to combat angiogenesis in tumors and also in other disease processes. ..