NUMA1

Summary

Gene Symbol: NUMA1
Description: nuclear mitotic apparatus protein 1
Alias: NMP-22, NUMA, nuclear mitotic apparatus protein 1, SP-H antigen, centrophilin stabilizes mitotic spindle in mitotic cells, nuclear matrix protein-22, structural nuclear protein
Species: human
Products:     NUMA1

Top Publications

  1. Tang T, Tang C, Chao Y, Wu C. Nuclear mitotic apparatus protein (NuMA): spindle association, nuclear targeting and differential subcellular localization of various NuMA isoforms. J Cell Sci. 1994;107 ( Pt 6):1389-402 pubmed
    We have recently shown that the nuclear mitotic apparatus protein (NuMA) is composed of at least three isoforms that differ mainly at the carboxy terminus, and are generated by alternative splicing of a common mRNA precursor from a single ..
  2. Maekawa T, Kuriyama R. Primary structure and microtubule-interacting domain of the SP-H antigen: a mitotic MAP located at the spindle pole and characterized as a homologous protein to NuMA. J Cell Sci. 1993;105 ( Pt 2):589-600 pubmed
    ..Comparison with other sequences in the database shows that the SP-H antigen is identical to the NuMA protein reported by Yang et al. (1992) J. Cell Biol...
  3. Abad P, Lewis J, Mian I, Knowles D, Sturgis J, Badve S, et al. NuMA influences higher order chromatin organization in human mammary epithelium. Mol Biol Cell. 2007;18:348-61 pubmed
    The coiled-coil protein NuMA is an important contributor to mitotic spindle formation and stabilization...
  4. Merdes A, Ramyar K, Vechio J, Cleveland D. A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly. Cell. 1996;87:447-58 pubmed
    b>NuMA is a nuclear protein during interphase but redistributes to the spindle poles early in mitosis. To investigate its role during spindle formation, we tested spindle assembly in frog egg extracts from which NuMA was immunodepleted...
  5. Du Q, Taylor L, Compton D, Macara I. LGN blocks the ability of NuMA to bind and stabilize microtubules. A mechanism for mitotic spindle assembly regulation. Curr Biol. 2002;12:1928-33 pubmed
    ..In mammalian cells, LGN binds with high affinity to the C-terminal tail of NuMA, a large nuclear protein that is required for spindle organization, and accumulates at the spindle poles during ..
  6. Merdes A, Heald R, Samejima K, Earnshaw W, Cleveland D. Formation of spindle poles by dynein/dynactin-dependent transport of NuMA. J Cell Biol. 2000;149:851-62 pubmed
    b>NuMA is a large nuclear protein whose relocation to the spindle poles is required for bipolar mitotic spindle assembly...
  7. Compton D, Luo C. Mutation of the predicted p34cdc2 phosphorylation sites in NuMA impair the assembly of the mitotic spindle and block mitosis. J Cell Sci. 1995;108 ( Pt 2):621-33 pubmed
    b>NuMA is a 236 kDa intranuclear protein that is distributed into each daughter cell during mitosis through association with the pericentrosomal region of the mitotic spindle...
  8. Taimen P, Viljamaa M, Kallajoki M. Preferential expression of NuMA in the nuclei of proliferating cells. Exp Cell Res. 2000;256:140-9 pubmed
    Nuclear mitotic apparatus protein (NuMA) has an indispensable function in normal mitosis as an organizer of the mitotic spindle...
  9. Wong R, Blobel G, Coutavas E. Rae1 interaction with NuMA is required for bipolar spindle formation. Proc Natl Acad Sci U S A. 2006;103:19783-7 pubmed
    ..The Nuclear Mitotic Apparatus protein (NuMA) is recruited from interphase nuclei to spindle MTs during mitosis...

More Information

Publications84

  1. Quintyne N, Reing J, Hoffelder D, Gollin S, Saunders W. Spindle multipolarity is prevented by centrosomal clustering. Science. 2005;307:127-9 pubmed
    ..The microtubule motor cytoplasmic dynein was a critical part of this coalescing machinery, and in some tumor cells overexpression of the spindle protein NuMA interfered with dynein localization, promoting multipolarity.
  2. Compton D, Szilak I, Cleveland D. Primary structure of NuMA, an intranuclear protein that defines a novel pathway for segregation of proteins at mitosis. J Cell Biol. 1992;116:1395-408 pubmed
    ..Since the cDNA also encodes the epitopes recognized by antibodies specific for two previously described proteins, NuMA and centrophilin, and all three show similar molecular weights and localization during the cell cycle, NuMA, ..
  3. Radulescu A, Cleveland D. NuMA after 30 years: the matrix revisited. Trends Cell Biol. 2010;20:214-22 pubmed publisher
    The large nuclear mitotic apparatus (NuMA) protein is an abundant component of interphase nuclei and an essential player in mitotic spindle assembly and maintenance...
  4. Mattagajasingh S, Huang S, Hartenstein J, Snyder M, Marchesi V, Benz E. A nonerythroid isoform of protein 4.1R interacts with the nuclear mitotic apparatus (NuMA) protein. J Cell Biol. 1999;145:29-43 pubmed
    ..1R isoform specifically interacts with the nuclear mitotic apparatus (NuMA) protein. NuMA and 4...
  5. Zheng Z, Wan Q, Liu J, Zhu H, Chu X, Du Q. Evidence for dynein and astral microtubule-mediated cortical release and transport of G?i/LGN/NuMA complex in mitotic cells. Mol Biol Cell. 2013;24:901-13 pubmed publisher
    ..We provide evidence for dynein- and astral microtubule-mediated transport of G?i/LGN/nuclear mitotic apparatus (NuMA) complex from cell cortex to spindle poles and show that actin filaments counteract such transport by maintaining G?..
  6. Kiyomitsu T, Cheeseman I. Cortical dynein and asymmetric membrane elongation coordinately position the spindle in anaphase. Cell. 2013;154:391-402 pubmed publisher
    ..motor dynein, which we demonstrate is targeted to the cell cortex by two distinct pathways: a G?i/LGN/NuMA-dependent pathway and a 4.1G/R and NuMA-dependent, anaphase-specific pathway...
  7. Haren L, Merdes A. Direct binding of NuMA to tubulin is mediated by a novel sequence motif in the tail domain that bundles and stabilizes microtubules. J Cell Sci. 2002;115:1815-24 pubmed
    In mitosis, NuMA localises to spindle poles where it contributes to the formation and maintenance of focussed microtubule arrays. Previous work has shown that NuMA is transported to the poles by dynein and dynactin...
  8. Harborth J, Wang J, Gueth Hallonet C, Weber K, Osborn M. Self assembly of NuMA: multiarm oligomers as structural units of a nuclear lattice. EMBO J. 1999;18:1689-700 pubmed
    b>NuMA is a nuclear matrix protein in interphase and relocates to the spindle poles in mitotis...
  9. Du Q, Macara I. Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins. Cell. 2004;119:503-16 pubmed
    ..called LGN, binds Galphai and also interacts through an N-terminal domain with the microtubule binding protein NuMA. During mitosis, LGN recruits NuMA to the cell cortex, while cortical association of LGN itself requires the C-..
  10. Silk A, Holland A, Cleveland D. Requirements for NuMA in maintenance and establishment of mammalian spindle poles. J Cell Biol. 2009;184:677-90 pubmed publisher
    ..By construction and analysis of a conditional loss of mitotic function allele of the nuclear mitotic apparatus (NuMA) protein in mice and cultured primary cells, we demonstrate that NuMA is an essential mitotic component with ..
  11. Harborth J, Weber K, Osborn M. GAS41, a highly conserved protein in eukaryotic nuclei, binds to NuMA. J Biol Chem. 2000;275:31979-85 pubmed
    The yeast two-hybrid system was used to identify binding partners of NuMA, a component of the nuclear matrix in interphase cells...
  12. Wiese C, Wilde A, Moore M, Adam S, Merdes A, Zheng Y. Role of importin-beta in coupling Ran to downstream targets in microtubule assembly. Science. 2001;291:653-6 pubmed
    ..A carboxyl-terminal region of the nuclear-mitotic apparatus protein (NuMA), a nuclear protein required for organizing mitotic spindle poles, mimics Ran's ability to induce asters...
  13. Chang W, Dynek J, Smith S. NuMA is a major acceptor of poly(ADP-ribosyl)ation by tankyrase 1 in mitosis. Biochem J. 2005;391:177-84 pubmed
    ..We identified NuMA (nuclear mitotic apparatus protein) as a major acceptor of poly(ADP-ribose) from tankyrase 1 in mitosis...
  14. Woodard G, Huang N, Cho H, Miki T, Tall G, Kehrl J. Ric-8A and Gi alpha recruit LGN, NuMA, and dynein to the cell cortex to help orient the mitotic spindle. Mol Cell Biol. 2010;30:3519-30 pubmed publisher
    ..Each treatment impaired the localization of LGN (GSPM2), NuMA (microtubule binding nuclear mitotic apparatus protein), and dynein at the metaphase cell cortex and disturbed ..
  15. Kotak S, Busso C, GONCZY P. NuMA phosphorylation by CDK1 couples mitotic progression with cortical dynein function. EMBO J. 2013;32:2517-29 pubmed publisher
    ..In human cells, an evolutionary conserved ternary complex (NuMA/LGN/G?i) anchors dynein at the cortex during metaphase, thus ensuring correct spindle positioning...
  16. Kotak S, Busso C, G nczy P. Cortical dynein is critical for proper spindle positioning in human cells. J Cell Biol. 2012;199:97-110 pubmed publisher
    ..Dynein and an evolutionarily conserved ternary complex (nuclear mitotic apparatus protein [NuMA]-LGN-G? in human cells and LIN-5-GPR-1/2-G? in Caenorhabditis elegans) are required for correct spindle positioning,..
  17. Kammerer S, Roth R, Hoyal C, Reneland R, Marnellos G, Kiechle M, et al. Association of the NuMA region on chromosome 11q13 with breast cancer susceptibility. Proc Natl Acad Sci U S A. 2005;102:2004-9 pubmed
    ..approximately 300 kb and spans several genes, including the gene encoding the nuclear mitotic apparatus protein (NuMA)...
  18. Zhu J, Wen W, Zheng Z, Shang Y, Wei Z, Xiao Z, et al. LGN/mInsc and LGN/NuMA complex structures suggest distinct functions in asymmetric cell division for the Par3/mInsc/LGN and G?i/LGN/NuMA pathways. Mol Cell. 2011;43:418-31 pubmed publisher
    ..Evidence from invertebrates demonstrates that the Par3/Par6/aPKC and NuMA/LGN/G?i complexes, which are thought to be physically linked by the adaptor protein mInscuteable (mInsc), play ..
  19. Sparks C, Bangs P, McNeil G, Lawrence J, Fey E. Assignment of the nuclear mitotic apparatus protein NuMA gene to human chromosome 11q13. Genomics. 1993;17:222-4 pubmed
    ..The sequence for this protein was shown to be identical to that of NuMA, a 236-kDa nuclear mitotic spindle apparatus protein...
  20. Tang T, Tang C, Chen Y, Wu C. Nuclear proteins of the bovine esophageal epithelium. II. The NuMA gene gives rise to multiple mRNAs and gene products reactive with monoclonal antibody W1. J Cell Sci. 1993;104 ( Pt 2):249-60 pubmed
    ..span the entire coding region, revealed that the W1 polypeptide was identical to the Nuclear Mitotic Apparatus (NuMA) protein, with a long alpha-helical central core flanked by two nonhelical domains...
  21. Du Q, Stukenberg P, Macara I. A mammalian Partner of inscuteable binds NuMA and regulates mitotic spindle organization. Nat Cell Biol. 2001;3:1069-75 pubmed
    ..We found that LGN binds the nuclear mitotic apparatus protein NuMA, which tethers spindles at the poles, and that this interaction is required for the LGN phenotype...
  22. Lydersen B, Pettijohn D. Human-specific nuclear protein that associates with the polar region of the mitotic apparatus: distribution in a human/hamster hybrid cell. Cell. 1980;22:489-99 pubmed
    ..This protein has been termed the nuclear-mitotic apparatus (NuMA) protein...
  23. Kiyomitsu T, Cheeseman I. Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation. Nat Cell Biol. 2012;14:311-7 pubmed publisher
    ..localization by controlling the interaction between dynein-dynactin and its upstream cortical targeting factors NuMA and LGN...
  24. Cox A, Dunning A, Garcia Closas M, Balasubramanian S, Reed M, Pooley K, et al. A common coding variant in CASP8 is associated with breast cancer risk. Nat Genet. 2007;39:352-8 pubmed
    ..ATM S49C (rs1800054), ADH1B 3' UTR A --> G (rs1042026), CDKN1A S31R (rs1801270), ICAM5 V301I (rs1056538) and NUMA1 A794G (rs3750913). We included data from 9-15 studies, comprising 11,391-18,290 cases and 14,753-22,670 controls...
  25. Yang C, Lambie E, Snyder M. NuMA: an unusually long coiled-coil related protein in the mammalian nucleus. J Cell Biol. 1992;116:1303-17 pubmed
    ..one of these sera corresponds to a previously identified protein called the nuclear mitotic apparatus protein (NuMA)...
  26. Wells R, Catzavelos C, Kamel Reid S. Fusion of retinoic acid receptor alpha to NuMA, the nuclear mitotic apparatus protein, by a variant translocation in acute promyelocytic leukaemia. Nat Genet. 1997;17:109-13 pubmed
    ..fourth APL gene fusion, which links exons encoding the retinoic acid and DNA-binding domains of RARA to 5' exons of NuMA, a gene that encodes the nuclear mitotic apparatus protein...
  27. Kisurina Evgenieva O, Mack G, Du Q, Macara I, Khodjakov A, Compton D. Multiple mechanisms regulate NuMA dynamics at spindle poles. J Cell Sci. 2004;117:6391-400 pubmed
    The large coiled-coil protein NuMA plays an essential role in organizing microtubule minus ends at spindle poles in vertebrate cells. Here, we use both in vivo and in vitro methods to examine NuMA dynamics at mitotic spindle poles...
  28. Clark I, Meyer D. Overexpression of normal and mutant Arp1alpha (centractin) differentially affects microtubule organization during mitosis and interphase. J Cell Sci. 1999;112 ( Pt 20):3507-18 pubmed
    ..Intracellular dynactin, dynein and nuclear/mitotic apparatus (NuMA) protein were recruited to multiple foci associated with ectopic cytoplasmic aggregates of Arp1alpha in transfected ..
  29. Panagopoulos I, Gorunova L, Bjerkehagen B, Lobmaier I, Heim S. LAMTOR1-PRKCD and NUMA1-SFMBT1 fusion genes identified by RNA sequencing in aneurysmal benign fibrous histiocytoma with t(3;11)(p21;q13). Cancer Genet. 2015;208:545-51 pubmed publisher
    ..showed that the t(3;11) generated two fusion genes: LAMTOR1-PRKCD and NUMA1-SFMBT1. RT-PCR together with Sanger sequencing verified the presence of fusion transcripts from both fusion genes...
  30. Luiz A, Wood J. Sodium Channels in Pain and Cancer: New Therapeutic Opportunities. Adv Pharmacol. 2016;75:153-78 pubmed publisher
    ..of Hodgkin and Huxley, they were subsequently identified at the molecular level by groups led by Catterall and Numa. VGSC dysfunction has long been linked to neuronal and cardiac disorders with some nonselective sodium channel ..
  31. Yamaki I, Pontes R, Costa F, Yamaki V, Teixeira R, Yasojima E, et al. Kidney ischemia and reperfunsion syndrome: effect of lidocaine and local postconditioning. Rev Col Bras Cir. 2016;43:348-353 pubmed publisher
    ..Nos grupos salina e lidocaína foram instiladas as substâncias numa taxa de duas gotas por minuto...
  32. Christophorou N, Rubin T, Bonnet I, Piolot T, Arnaud M, Huynh J. Microtubule-driven nuclear rotations promote meiotic chromosome dynamics. Nat Cell Biol. 2015;17:1388-400 pubmed publisher
    ..We identified Mud (NuMA in vertebrates) as co-localizing with centromeres, Klarsicht and Klaroid...
  33. Heppert J, Pani A, Roberts A, Dickinson D, Goldstein B. A CRISPR Tagging-Based Screen Reveals Localized Players in Wnt-Directed Asymmetric Cell Division. Genetics. 2018;208:1147-1164 pubmed publisher
    ..in response to a Wnt signal, but two proteins typically associated with mitotic spindle positioning, homologs of NuMA and Dynein, were not detectably polarized...
  34. Menicucci T, Costa L, Machado J. Health pact: approaches and collisions in the federal arena. Cien Saude Colet. 2018;23:29-40 pubmed publisher
    ..Ainda que considerando que tal processo se desenvolveu de forma privilegiada, numa arena federativa paritária – a CIT –, assume-se a hipótese geral da literatura brasileira sobre federalismo ..
  35. di Pietro F, Echard A, Morin X. Regulation of mitotic spindle orientation: an integrated view. EMBO Rep. 2016;17:1106-30 pubmed publisher
    ..Early studies identified the evolutionarily conserved G?i/LGN/NuMA complex as a key regulator that polarizes cortical force generators...
  36. Wieben E, Aleff R, Tang X, Butz M, Kalari K, HIGHSMITH E, et al. Trinucleotide Repeat Expansion in the Transcription Factor 4 (TCF4) Gene Leads to Widespread mRNA Splicing Changes in Fuchs' Endothelial Corneal Dystrophy. Invest Ophthalmol Vis Sci. 2017;58:343-352 pubmed publisher
    ..Differential splicing of NUMA1, PPFIBP1, MBNL1, and MBNL2 transcripts were identified in all FECD samples containing a TNR expansion...
  37. Chiu C, Monat C, Robitaille M, Lacomme M, Daulat A, Macleod G, et al. SAPCD2 Controls Spindle Orientation and Asymmetric Divisions by Negatively Regulating the Gαi-LGN-NuMA Ternary Complex. Dev Cell. 2016;36:50-62 pubmed publisher
    ..Proper spatiotemporal localization of the evolutionarily conserved Gαi-LGN-NuMA protein complex is critical for mitotic spindle orientation, but how this is achieved remains unclear...
  38. Matias M, Casa Nova M, Faria M, Pires R, Tato Costa J, Ribeiro L, et al. Prognostic Factors after Liver Resection for Colorectal Liver Metastasis. Acta Med Port. 2015;28:357-69 pubmed
    ..tratamento potencialmente curativo em doentes com metástases hepáticas de cancro colo-rectal, resultando numa sobrevida global de 36-58%...
  39. Paine H, Nathubhai A, Woon E, Sunderland P, Wood P, Mahon M, et al. Exploration of the nicotinamide-binding site of the tankyrases, identifying 3-arylisoquinolin-1-ones as potent and selective inhibitors in vitro. Bioorg Med Chem. 2015;23:5891-908 pubmed publisher
    ..Using NAD(+) as a substrate, they poly(ADP-ribosyl)ate TRF1 (regulating lengths of telomeres), NuMA (facilitating mitosis) and axin (in wnt/β-catenin signalling)...
  40. Magescas J, Sengmanivong L, Viau A, Mayeux A, Dang T, Burtin M, et al. Spindle pole cohesion requires glycosylation-mediated localization of NuMA. Sci Rep. 2017;7:1474 pubmed publisher
    ..Here we find that Galectin-3 is essential for metaphase where it associates with NuMA in an O-GlcNAcylation-dependent manner...
  41. Stuurman N, Haner M, Sasse B, Hubner W, Suter B, Aebi U. Interactions between coiled-coil proteins: Drosophila lamin Dm0 binds to the bicaudal-D protein. Eur J Cell Biol. 1999;78:278-87 pubmed
    In a yeast two-hybrid screen we identified an interaction between Drosophila lamin Dm0, a structural nuclear protein, and BICD, a protein involved in oocyte development...
  42. Taimen P, Kallajoki M. NuMA and nuclear lamins behave differently in Fas-mediated apoptosis. J Cell Sci. 2003;116:571-83 pubmed
    b>NuMA is a nuclear matrix protein that has an essential function in the organization of the mitotic spindle. Here we have studied the fate of NuMA in Fas-treated apoptotic Jurkat T and HeLa cells...
  43. Jovanovic M, Soldatovic I, Janjic A, Vuksanovic A, Dzamic Z, Acimovic M, et al. Diagnostic value of the nuclear matrix protein 22 test and urine cytology in upper tract urothelial tumors. Urol Int. 2011;87:134-7 pubmed publisher
    To investigate the diagnostic value of the nuclear matrix protein 22 (NMP22) test in comparison to urine cytology for the detection of upper tract urothelial carcinoma...
  44. Brüning Richardson A, Bond J, Alsiary R, Richardson J, Cairns D, McCormac L, et al. NuMA overexpression in epithelial ovarian cancer. PLoS ONE. 2012;7:e38945 pubmed publisher
    Highly aneuploid tumours are common in epithelial ovarian cancers (EOC). We investigated whether NuMA expression was associated with this phenomenon...
  45. Tateda Y, Tsumune D, Tsubono T, Aono T, Kanda J, Ishimaru T. Radiocesium biokinetics in olive flounder inhabiting the Fukushima accident-affected Pacific coastal waters of eastern Japan. J Environ Radioact. 2015;147:130-41 pubmed publisher
    ..The (137)Cs concentrations in seawater of the Pacific coastal sites of eastern Japan, from Kesen-numa (170 km north from the 1FNPP) to Choshi (190 km south from the 1FNPP), were reconstructed by fitting the ..
  46. Pillai S, Nguyen J, Johnson J, Haura E, Coppola D, Chellappan S. Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis. Nat Commun. 2015;6:10072 pubmed publisher
    ..TBK1 binds to the centrosomal protein CEP170 and to the mitotic apparatus protein NuMA, and both CEP170 and NuMA are TBK1 substrates...
  47. Vega S, Liu E, Arvind V, Bushman J, Sung H, Becker M, et al. High-content image informatics of the structural nuclear protein NuMA parses trajectories for stem/progenitor cell lineages and oncogenic transformation. Exp Cell Res. 2017;351:11-23 pubmed publisher
    ..analysis of parsing outcomes via either nuclear shape or textural metrics of the nuclear structural protein NuMA indicates the nuclear shape alone is a weak phenotypic predictor...
  48. Li S, Wang H. Tug of War in Spindle Orientation. Dev Cell. 2016;36:1-2 pubmed publisher
    The evolutionarily conserved Gαi-LGN-NuMA protein complex is a key regulator of spindle orientation, but how its spatiotemporal localization is achieved remains elusive. Chiu et al...
  49. Lee B, Schwager F, Meraldi P, Gotta M. p37/UBXN2B regulates spindle orientation by limiting cortical NuMA recruitment via PP1/Repo-Man. J Cell Biol. 2018;217:483-493 pubmed publisher
    ..In animal cells, spindle orientation is regulated by the conserved Gαi-LGN-NuMA complex, which targets the force generator dynein-dynactin to the cortex...
  50. Wang X, Yang Y, Duan Q, Jiang N, Huang Y, Darzynkiewicz Z, et al. sSgo1, a major splice variant of Sgo1, functions in centriole cohesion where it is regulated by Plk1. Dev Cell. 2008;14:331-41 pubmed publisher
    ..Our studies strongly suggest that sSgo1 plays an essential role in protecting centriole cohesion, which is partly regulated by Plk1. ..
  51. Ohta S, Wood L, Toramoto I, Yagyu K, Fukagawa T, Earnshaw W. CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly. Mol Biol Cell. 2015;26:1225-37 pubmed publisher
    ..Additional data suggest that NuMA tethers microtubules at the anastral spindle poles and that augmin is required for centrosome detachment after CENP-..
  52. Schmitt J, Fischer U, Heisel S, Strickfaden H, Backes C, Ruggieri A, et al. GAS41 amplification results in overexpression of a new spindle pole protein. Genes Chromosomes Cancer. 2012;51:868-80 pubmed publisher
    ..This number was even increased by a combined overexpression of GAS41 and a reduced expression of NuMA. We propose that GAS41 amplification may have an effect on the highly altered karyotype of glioblastoma via its ..
  53. Ko L, Chin W. Nuclear receptor coactivator thyroid hormone receptor-binding protein (TRBP) interacts with and stimulates its associated DNA-dependent protein kinase. J Biol Chem. 2003;278:11471-9 pubmed
    ..The activation of DNA-PK in the absence of DNA ends by the coactivator TRBP suggests a novel mechanism of coactivator-stimulated DNA-PK phosphorylation in transcriptional regulation. ..
  54. Kolano A, Brunet S, Silk A, Cleveland D, Verlhac M. Error-prone mammalian female meiosis from silencing the spindle assembly checkpoint without normal interkinetochore tension. Proc Natl Acad Sci U S A. 2012;109:E1858-67 pubmed publisher
    ..Cre recombinase-mediated removal in oocytes of the microtubule binding site of nuclear mitotic apparatus protein (NuMA), which is implicated in anchoring microtubules at poles, we determine that without functional NuMA, microtubules ..
  55. Endo A, Moyori A, Kobayashi A, Wong R. Nuclear mitotic apparatus protein, NuMA, modulates p53-mediated transcription in cancer cells. Cell Death Dis. 2013;4:e713 pubmed publisher
  56. Alvarez Sedó C, Schatten H, Combelles C, Rawe V. The nuclear mitotic apparatus (NuMA) protein: localization and dynamics in human oocytes, fertilization and early embryos. Mol Hum Reprod. 2011;17:392-8 pubmed publisher
    ..Disorganization of centrosomal proteins, including the nuclear mitotic apparatus (NuMA) protein and the molecular motor complex dynein/dynactin, can lead to chromosomal instability and developmental ..
  57. Delhommeau F, Vasseur Godbillon C, Leclerc P, Schischmanoff P, Croisille L, Rince P, et al. A splicing alteration of 4.1R pre-mRNA generates 2 protein isoforms with distinct assembly to spindle poles in mitotic cells. Blood. 2002;100:2629-36 pubmed
    ..1R, encoded by exons 20 and 21, contains a binding site for nuclear mitotic apparatus protein (NuMA), a protein needed for the formation and stabilization of the mitotic spindle...
  58. Ye K, Compton D, Lai M, Walensky L, Snyder S. Protein 4.1N binding to nuclear mitotic apparatus protein in PC12 cells mediates the antiproliferative actions of nerve growth factor. J Neurosci. 1999;19:10747-56 pubmed
    ..1R. In the present study, we demonstrate an interaction between 4.1N and nuclear mitotic apparatus protein (NuMA), a nuclear protein required for mitosis. The binding involves the C-terminal domain of 4.1N...
  59. Bosveld F, Ainslie A, Bellaiche Y. Sequential activities of Dynein, Mud and Asp in centrosome-spindle coupling maintain centrosome number upon mitosis. J Cell Sci. 2017;130:3557-3567 pubmed publisher
    ..The conserved NuMA protein (Mud in Drosophila) is best known for its role in spindle orientation...
  60. Kotak S, Afshar K, Busso C, GONCZY P. Aurora A kinase regulates proper spindle positioning in C. elegans and in human cells. J Cell Sci. 2016;129:3015-25 pubmed publisher
    ..There, Aurora A is needed for the cortical localization of NuMA and dynein during mitosis...
  61. Liro M, Rose L. Mitotic Spindle Positioning in the EMS Cell of Caenorhabditis elegans Requires LET-99 and LIN-5/NuMA. Genetics. 2016;204:1177-1189 pubmed publisher
    ..In many cell types, a cortically localized complex consisting of G?, GPR-1/2, and LIN-5 (G?i/Pins/Mud, G?i/LGN/NuMA) mediates the recruitment of dynactin/dynein, which exerts pulling forces on astral microtubules to physically ..
  62. Elting M, Prakash M, Udy D, Dumont S. Mapping Load-Bearing in the Mammalian Spindle Reveals Local Kinetochore Fiber Anchorage that Provides Mechanical Isolation and Redundancy. Curr Biol. 2017;27:2112-2122.e5 pubmed publisher
    ..The microtubule crosslinker NuMA is needed for the local load-bearing observed, whereas Eg5 and PRC1 are not detectably required, suggesting ..
  63. Connell M, Chen H, Jiang J, Kuan C, Fotovati A, Chu T, et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development. elife. 2017;6: pubmed publisher
    ..Common to most cell types is the asymmetric establishment and regulation of cortical NuMA-dynein complexes that position the mitotic spindle...
  64. Hsu H, Yeh N. Dynamic changes of NuMA during the cell cycle and possible appearance of a truncated form of NuMA during apoptosis. J Cell Sci. 1996;109 ( Pt 2):277-88 pubmed
    We have demonstrated that dynamic redistribution of nuclear-mitotic apparatus (NuMA) protein in the cell cycle is correlated temporally and spatially with its biochemical modifications...
  65. di Pietro F, Valon L, Li Y, Goïame R, Genovesio A, Morin X. An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z ? as an Essential Regulator of Spindle Orientation. Curr Biol. 2017;27:2452-2464.e8 pubmed publisher
    Oriented cell divisions are controlled by a conserved molecular cascade involving G?i, LGN, and NuMA. We developed a new cellular model of oriented cell divisions combining micropatterning and localized recruitment of G?i and performed an ..
  66. Jayaraman S, Chittiboyina S, Bai Y, Abad P, Vidi P, Stauffacher C, et al. The nuclear mitotic apparatus protein NuMA controls rDNA transcription and mediates the nucleolar stress response in a p53-independent manner. Nucleic Acids Res. 2017;45:11725-11742 pubmed publisher
    The nuclear mitotic apparatus protein, NuMA, is involved in major cellular events such as DNA damage response, apoptosis and p53-mediated growth-arrest, all of which are under the control of the nucleolus upon stress...
  67. Gallini S, Carminati M, De Mattia F, Pirovano L, Martini E, Oldani A, et al. NuMA Phosphorylation by Aurora-A Orchestrates Spindle Orientation. Curr Biol. 2016;26:458-69 pubmed publisher
    ..network synchronizing spindle placement with mitotic progression relies on timely recruitment at the cell cortex of NuMA:LGN:Gαi complexes, in which NuMA acts as a receptor for the microtubule motor Dynein...
  68. Chang P, Coughlin M, Mitchison T. Interaction between Poly(ADP-ribose) and NuMA contributes to mitotic spindle pole assembly. Mol Biol Cell. 2009;20:4575-85 pubmed publisher
    ..Candidate binding proteins included the spindle pole protein NuMA previously shown to bind to PARP-5a directly...
  69. Visochek L, Castiel A, Mittelman L, Elkin M, Atias D, Golan T, et al. Exclusive destruction of mitotic spindles in human cancer cells. Oncotarget. 2017;8:20813-20824 pubmed publisher
    ..in a variety of human cancer cells is attributed by these findings to post translational modifications of NuMA and kinesins HSET/kifC1 and kif18A...
  70. Chu X, Chen X, Wan Q, Zheng Z, Du Q. Nuclear Mitotic Apparatus (NuMA) Interacts with and Regulates Astrin at the Mitotic Spindle. J Biol Chem. 2016;291:20055-67 pubmed publisher
    The large nuclear mitotic apparatus (NuMA) protein is an essential player in mitotic spindle assembly and maintenance...
  71. Davies R, Wagstaff K, McLaughlin E, Loveland K, Jans D. The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention factor for nuclear and nuclear envelope-localizing testicular proteins. Biochim Biophys Acta. 2013;1833:3436-3444 pubmed publisher
    ..the high mobility group (HMG)-box-domain-containing chromatin component HMG20A, nuclear mitotic apparatus protein NuMA1 and synaptic nuclear envelope protein SYNE2...
  72. Cassimeris L, Morabito J. TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly. Mol Biol Cell. 2004;15:1580-90 pubmed
    ..TOGp depletion also disrupted MT minus-end focus at the spindle poles, detected by localizations of NuMA and the p150 component of dynactin...
  73. Ban K, Torres J, Miller J, Mikhailov A, Nachury M, Tung J, et al. The END network couples spindle pole assembly to inhibition of the anaphase-promoting complex/cyclosome in early mitosis. Dev Cell. 2007;13:29-42 pubmed
    ..Here we describe a critical spindle pole-associated mechanism, called the END (Emi1/NuMA/dynein-dynactin) network, that spatially restricts APC/C activity in early mitosis...
  74. Emanuele M, Ciccia A, Elia A, Elledge S. Proliferating cell nuclear antigen (PCNA)-associated KIAA0101/PAF15 protein is a cell cycle-regulated anaphase-promoting complex/cyclosome substrate. Proc Natl Acad Sci U S A. 2011;108:9845-50 pubmed publisher
    ..We conclude that PAF15 is an APC/C-regulated protein involved in both cell cycle progression and the DNA damage response. ..
  75. Novick D, Kim S, Fantuzzi G, Reznikov L, Dinarello C, Rubinstein M. Interleukin-18 binding protein: a novel modulator of the Th1 cytokine response. Immunity. 1999;10:127-36 pubmed
    ..3 kb genomic sequence. Several Poxviruses encode putative proteins highly homologous to IL-18BP, suggesting that viral products may attenuate IL-18 and interfere with the cytotoxic T cell response. ..