nucleostemin

Summary

Gene Symbol: nucleostemin
Description: G protein nucleolar 3
Alias: C77032, E2IG3, NNP47, guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, estradiol-induced nucleotide binding protein, guanine nucleotide binding protein-like 3 (nucleolar), novel nucleolar protein 47, nucleolar GTP-binding protein 3, nucleostemin
Species: human
Products:     nucleostemin

Top Publications

  1. Tsai R, McKay R. A nucleolar mechanism controlling cell proliferation in stem cells and cancer cells. Genes Dev. 2002;16:2991-3003 pubmed
    ..In the present study, we identify a novel protein, nucleostemin, found in the nucleoli of CNS stem cells, embryonic stem cells, and several cancer cell lines and ..
  2. Maida Y, Yasukawa M, Okamoto N, Ohka S, Kinoshita K, Totoki Y, et al. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Mol Cell Biol. 2014;34:1576-93 pubmed publisher
    ..Here, we describe a complex composed of human TERT (hTERT), Brahma-related gene 1 (BRG1), and nucleostemin (NS) that contributes to heterochromatin maintenance at centromeres and transposons...
  3. Dai M, Sun X, Lu H. Aberrant expression of nucleostemin activates p53 and induces cell cycle arrest via inhibition of MDM2. Mol Cell Biol. 2008;28:4365-76 pubmed publisher
    The nucleolar protein nucleostemin (NS) is essential for cell proliferation and early embryogenesis. Both depletion and overexpression of NS reduce cell proliferation. However, the mechanisms underlying this regulation are still unclear...
  4. Kafienah W, Mistry S, Williams C, Hollander A. Nucleostemin is a marker of proliferating stromal stem cells in adult human bone marrow. Stem Cells. 2006;24:1113-20 pubmed
    ..b>Nucleostemin is a recently discovered nucleolar protein predominantly associated with proliferating rat neural and embryonic ..
  5. Zeggini E, Panoutsopoulou K, Southam L, Rayner N, Day Williams A, Lopes M, et al. Identification of new susceptibility loci for osteoarthritis (arcOGEN): a genome-wide association study. Lancet. 2012;380:815-23 pubmed publisher
    ..This SNP encodes a missense polymorphism within the nucleostemin-encoding gene GNL3...
  6. Zhu Q, Yasumoto H, Tsai R. Nucleostemin delays cellular senescence and negatively regulates TRF1 protein stability. Mol Cell Biol. 2006;26:9279-90 pubmed
    b>Nucleostemin (NS) encodes a nucleolar GTP-binding protein highly enriched in the stem cells and cancer cells. To determine its biological activity in vivo, we generated NS loss- and gain-of-function mouse models...
  7. Liu S, Cai Z, Liu Y, Dong M, Sun L, Hu G, et al. Role of nucleostemin in growth regulation of gastric cancer, liver cancer and other malignancies. World J Gastroenterol. 2004;10:1246-9 pubmed
    To examine the role of nucleostemin in the growth regulation of gastric cancer, liver cancer and other cancers. RT-PCR was used to clone the fragment of nucleostemin cDNA from HEK 293 cells...
  8. Yoshida R, Fujimoto T, Kudoh S, Nagata M, Nakayama H, Shinohara M, et al. Nucleostemin affects the proliferation but not differentiation of oral squamous cell carcinoma cells. Cancer Sci. 2011;102:1418-23 pubmed publisher
    b>Nucleostemin (NS) has been reported as essential for stem and cancer cell proliferation...
  9. Ye F, Zhou C, Cheng Q, Shen J, Chen H. Stem-cell-abundant proteins Nanog, Nucleostemin and Musashi1 are highly expressed in malignant cervical epithelial cells. BMC Cancer. 2008;8:108 pubmed publisher
    Nanog, nucleostemin (NS) and musashi1 (Msi1) are proteins that are highly expressed in undifferentiated embryonic stem (ES) cells and have been shown to be essential in maintaining the pluripotency and regulating the proliferation and ..

More Information

Publications95

  1. Zhang G, Zhang Q, Zhang Q, Yin L, Li S, Cheng K, et al. Expression of nucleostemin, epidermal growth factor and epidermal growth factor receptor in human esophageal squamous cell carcinoma tissues. J Cancer Res Clin Oncol. 2010;136:587-94 pubmed publisher
    To determine the expression of nucleostemin (NS), epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) mRNA in human esophageal squamous cell carcinoma (ESCC) tissues and their association in a human ESCC cell line...
  2. Ma H, Pederson T. Depletion of the nucleolar protein nucleostemin causes G1 cell cycle arrest via the p53 pathway. Mol Biol Cell. 2007;18:2630-5 pubmed
    b>Nucleostemin (NS) is a nucleolar protein expressed in adult and embryo-derived stem cells, transformed cell lines, and tumors...
  3. Meng L, Hsu J, Zhu Q, Lin T, Tsai R. Nucleostemin inhibits TRF1 dimerization and shortens its dynamic association with the telomere. J Cell Sci. 2011;124:3706-14 pubmed publisher
    ..that TRF1 dimerization and its telomere binding can be increased by GNL3L, which is the vertebrate paralogue of nucleostemin (NS). Here, we show that NS and GNL3L bind TRF1 directly but competitively through two separate domains of TRF1...
  4. Meng L, Lin T, Tsai R. Nucleoplasmic mobilization of nucleostemin stabilizes MDM2 and promotes G2-M progression and cell survival. J Cell Sci. 2008;121:4037-46 pubmed publisher
    ..Here, we demonstrate that mobilization of nucleostemin, a nucleolar protein enriched in cancer and stem cells, has the opposite role of stabilizing MDM2 and ..
  5. Romanova L, Kellner S, Katoku Kikyo N, Kikyo N. Novel role of nucleostemin in the maintenance of nucleolar architecture and integrity of small nucleolar ribonucleoproteins and the telomerase complex. J Biol Chem. 2009;284:26685-94 pubmed publisher
    b>Nucleostemin (NS) is a nucleolar protein involved in the regulation of cell proliferation. Both overexpression and knockdown of NS increase the activity of the tumor suppressor protein p53, resulting in cell cycle arrest...
  6. Siddiqi S, Gude N, Hosoda T, Muraski J, Rubio M, Emmanuel G, et al. Myocardial induction of nucleostemin in response to postnatal growth and pathological challenge. Circ Res. 2008;103:89-97 pubmed publisher
    ..One such regulatory protein, named nucleostemin, has been studied in the context of stem cells and several cancer cell lines, where expression is associated ..
  7. Malakootian M, Mowla S, Saberi H, Asadi M, Atlasi Y, Shafaroudi A. Differential expression of nucleostemin, a stem cell marker, and its variants in different types of brain tumors. Mol Carcinog. 2010;49:818-25 pubmed publisher
    b>Nucleostemin (NS) is implicated in the control of stem and cancer cell proliferation. In the present study, we have examined the expression of NS and its spliced variants in various brain tumors...
  8. Fan Y, Liu Z, Zhao S, Lou F, Nilsson S, Ekman P, et al. Nucleostemin mRNA is expressed in both normal and malignant renal tissues. Br J Cancer. 2006;94:1658-62 pubmed
    b>Nucleostemin (NS), a p53-binding protein, has been shown essential for stem and cancer cell proliferation and implicated in oncogenesis...
  9. Okamoto N, Yasukawa M, Nguyen C, Kasim V, Maida Y, Possemato R, et al. Maintenance of tumor initiating cells of defined genetic composition by nucleostemin. Proc Natl Acad Sci U S A. 2011;108:20388-93 pubmed publisher
    ..Here, we show that overexpression of either of the nucleolar GTP-binding proteins nucleostemin (NS) or GNL3L drives the fraction of genetically defined tumor cells that exhibit markers and tumorigenic ..
  10. Nikpour P, Mowla S, Jafarnejad S, Fischer U, Schulz W. Differential effects of Nucleostemin suppression on cell cycle arrest and apoptosis in the bladder cancer cell lines 5637 and SW1710. Cell Prolif. 2009;42:762-9 pubmed publisher
    The Nucleostemin (NS) gene encodes a nucleolar protein enriched in adult and embryonic stem cells. NS is thought to regulate cancer cell proliferation, but the mechanisms involved are poorly understood...
  11. Sijin L, Ziwei C, Yajun L, Meiyu D, Hongwei Z, Guofa H, et al. The effect of knocking-down nucleostemin gene expression on the in vitro proliferation and in vivo tumorigenesis of HeLa cells. J Exp Clin Cancer Res. 2004;23:529-38 pubmed
    Previous studies have demonstrated nucleostemin (NS) expressed in undifferentiated cells (e.g. embryonic stem cells and myeloid stem cells) and various cancer cell lines, but didn't express in differentiated adult tissues...
  12. Tsai R, McKay R. A multistep, GTP-driven mechanism controlling the dynamic cycling of nucleostemin. J Cell Biol. 2005;168:179-84 pubmed
    b>Nucleostemin (NS) was identified as a stem cell- and cancer cell-enriched nucleolar protein that controls the proliferation of these cells...
  13. Romanova L, Grand A, Zhang L, Rayner S, Katoku Kikyo N, Kellner S, et al. Critical role of nucleostemin in pre-rRNA processing. J Biol Chem. 2009;284:4968-77 pubmed publisher
    b>Nucleostemin is a nucleolar protein widely expressed in proliferating cells...
  14. Lin T, Meng L, Li Y, Tsai R. Tumor-initiating function of nucleostemin-enriched mammary tumor cells. Cancer Res. 2010;70:9444-52 pubmed publisher
    b>Nucleostemin (NS) is highly expressed in normal stem cells and tumors and is upregulated by estradiol in MCF7 breast cancer cells...
  15. Zhu Q, Meng L, Hsu J, Lin T, Teishima J, Tsai R. GNL3L stabilizes the TRF1 complex and promotes mitotic transition. J Cell Biol. 2009;185:827-39 pubmed publisher
    ..Here, we identify guanine nucleotide-binding protein-like 3 (GNL3L), a GTP-binding protein most similar to nucleostemin, as a novel TRF1-interacting protein in vivo...
  16. Charpentier A, Bednarek A, Daniel R, Hawkins K, Laflin K, Gaddis S, et al. Effects of estrogen on global gene expression: identification of novel targets of estrogen action. Cancer Res. 2000;60:5977-83 pubmed
  17. Han C, Zhang X, Xu W, Wang W, Qian H, Chen Y. Cloning of the nucleostemin gene and its function in transforming human embryonic bone marrow mesenchymal stem cells into F6 tumor cells. Int J Mol Med. 2005;16:205-13 pubmed
    ..Results showed that the expression of nucleostemin, a novel factor participating in the control of stem and cancer cell proliferation, was different in F6 cells ..
  18. Nakajima T, Yoshida H, Okamoto N, Nagashima K, Taniguchi H, Yamada Y, et al. Nucleostemin and TWIST as predictive markers for recurrence after neoadjuvant chemotherapy for esophageal carcinoma. Cancer Sci. 2012;103:233-8 pubmed publisher
    We recently demonstrated that overexpression of the nucleolar GTP-binding protein nucleostemin drives the fraction of genetically-defined tumor cells that exhibit markers and tumorigenic properties of tumor initiating cells...
  19. Mazor M, Cesaro A, Ali M, Best T, Lespessaille E, Toumi H. Progenitor Cells from Cartilage: Grade Specific Differences in Stem Cell Marker Expression. Int J Mol Sci. 2017;18: pubmed publisher
    ..Furthermore, MSC markers: Nucleostemin, CD90, CD73, CD166, CD105 and Notch 1 were studied by immunofluorescence...
  20. Johnson P, Doe C, Lai S. Drosophila nucleostemin 3 is required to maintain larval neuroblast proliferation. Dev Biol. 2018;440:1-12 pubmed publisher
    ..what regulates stem cell proliferation versus differentiation? We performed a genetic screen which identified nucleostemin 3 (ns3) as a gene required to maintain neuroblast proliferation...
  21. Rosby R, Cui Z, Rogers E, deLivron M, Robinson V, DiMario P. Knockdown of the Drosophila GTPase nucleostemin 1 impairs large ribosomal subunit biogenesis, cell growth, and midgut precursor cell maintenance. Mol Biol Cell. 2009;20:4424-34 pubmed publisher
    Mammalian nucleostemin (NS) is a nucleolar guanosine triphosphate-binding protein implicated in cell cycle progression, stem cell proliferation, and ribosome assembly...
  22. Wang Z, Wang X, Xie B, Hong Z, Yang Q. Arabidopsis NUCLEOSTEMIN-LIKE 1 (NSN1) regulates cell cycling potentially by cooperating with nucleosome assembly protein AtNAP1;1. BMC Plant Biol. 2018;18:99 pubmed publisher
    In mammals, nucleostemin (NS), a nucleolar GTPase, is involved in stem cell proliferation, embryogenesis and ribosome biogenesis...
  23. Ho T, Tsai S, Yeh S, Chen S, Tung K, Chien H, et al. PEDF-derived peptide promotes tendon regeneration through its mitogenic effect on tendon stem/progenitor cells. Stem Cell Res Ther. 2019;10:2 pubmed publisher
    ..injured tendon and their proliferation were monitored using immunohistochemistry with antibodies to CD146 and nucleostemin and by BrdU labeling. TSPC markers were enriched among the primary tendon cells when stimulated by the 29-mer...
  24. Yoshida R, Nakayama H, Nagata M, Hirosue A, Tanaka T, Kawahara K, et al. Overexpression of nucleostemin contributes to an advanced malignant phenotype and a poor prognosis in oral squamous cell carcinoma. Br J Cancer. 2014;111:2308-15 pubmed publisher
    b>Nucleostemin (NS) is essential for the maintenance of stem cell properties, the functions of which remain poorly understood in cancer cells...
  25. Wei Y, Li Z, Zhang F, Wang N, Liu S, Yue B. [Effect of Down-Regulatory Nucleostemin Expression on Autophagy Activities in p53 Null HL-60 Leukemia Cells]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017;25:699-705 pubmed publisher
    Based on previous microarry and bioinformatic analysis results, to investigate the effect of nucleostemin(NS) expression down-regulation on autophagy activity in p53 null HL-60 leukemia cells, so as to provide evidence for studying ..
  26. Ding Z, Huang H. Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair. BMC Musculoskelet Disord. 2015;16:65 pubmed publisher
    ..They both express stem cell markers including SSEA-4, Nanog, nucleostemin, strol-1, CD44 and CD90. However, MMSCs differed from BMSCs...
  27. Liu R, Sun J, Zhang Z, Xu Y. Cell-selective gene silencing in prostate cancer LNCap cells using prostate-specific membrane antigen promoter and enhancer in vitro and in vivo. Cell Biol Int. 2012;36:863-72 pubmed publisher
    ..Then we chose NS (nucleostemin), an important endogenous gene of prostate cancer, and constructed the NS-targeting shRNA (small-hairpin RNA) ..
  28. Tancioni I, Miller N, Uryu S, Lawson C, Jean C, Chen X, et al. FAK activity protects nucleostemin in facilitating breast cancer spheroid and tumor growth. Breast Cancer Res. 2015;17:47 pubmed publisher
    ..The nucleolus is a non-membrane nuclear structure that regulates ribosome biogenesis and cell proliferation. Nucleostemin (NS), a nucleolar-localized protein, modulates cell cycle progression, stemness, and three-dimensional tumor ..
  29. Runesson E, Ackermann P, Karlsson J, Eriksson B. Nucleostemin- and Oct 3/4-positive stem/progenitor cells exhibit disparate anatomical and temporal expression during rat Achilles tendon healing. BMC Musculoskelet Disord. 2015;16:212 pubmed publisher
    ..with antibodies specific to stem/progenitor cells (Octamer-binding transcription factor 3/4 (Oct 3/4) and nucleostemin), migrating cells (Dynamin 2 (Dyn 2)) and leukocytes (CD45)...
  30. Hoshi N, Kusakabe T, Taylor B, Kimura S. Side population cells in the mouse thyroid exhibit stem/progenitor cell-like characteristics. Endocrinology. 2007;148:4251-8 pubmed
    ..RT-PCR analysis revealed that SP cells highly express ABCG2 and the stem cell marker genes encoding nucleostemin and Oct4, whereas the expression of genes encoding the thyroid differentiation markers, thyroid peroxidase, ..
  31. Samse K, Emathinger J, Hariharan N, Quijada P, Ilves K, Völkers M, et al. Functional Effect of Pim1 Depends upon Intracellular Localization in Human Cardiac Progenitor Cells. J Biol Chem. 2015;290:13935-47 pubmed publisher
    ..galactosidase activity, preserved telomere length, reduced expression of p16 and p53, and up-regulation of nucleostemin relative to PimWT hCPCs...
  32. Gui J, Zhang J, Huang H. Isolation and characterization of meniscus derived stem cells from rabbit as a possible treatment for damaged meniscus. Curr Stem Cell Res Ther. 2015;10:353-63 pubmed
    ..All of them were found to express high levels of stem cell markers including SSEA-4, Nanog and nucleostemin. High level of collagen II expression was detected in meniscus derived stem cells...
  33. Chen J, Dong S, Hu J, Duan B, Yao J, Zhang R, et al. Guanine nucleotide binding protein-like 3 is a potential prognosis indicator of gastric cancer. Int J Clin Exp Pathol. 2015;8:13273-8 pubmed
    ..Our study suggests that GNL3 might play an important role in the progression of gastric cancer and serve as a biomarker for poor prognosis in gastric cancer patients. ..
  34. Sang C, Cao X, Chen F, Yang X, Zhang Y. Differential Characterization of Two Kinds of Stem Cells Isolated from Rabbit Nucleus Pulposus and Annulus Fibrosus. Stem Cells Int. 2016;2016:8283257 pubmed
    ..i>Results. Both disk cells formed colonies in culture and expressed stem cell markers, nucleostemin, Oct-4, SSEA-4, and Stro-1, at early passages...
  35. Lacina L, Smetana K, Dvořánková B, Stork J, Plzáková Z, Gabius H. Immunocyto- and histochemical profiling of nucleostemin expression: marker of epidermal stem cells?. J Dermatol Sci. 2006;44:73-80 pubmed
    Because the nucleolar protein nucleostemin is present in bone marrow and neuronal stem cells and malignancies originating thereof we monitored its expression in frozen sections from normal human epidermis, basal cell carcinomas, cultured ..
  36. Lee M, Williams K, Hu Y, Andreas J, Patel S, Zhang S, et al. GNL3 and SKA3 are novel prostate cancer metastasis susceptibility genes. Clin Exp Metastasis. 2015;32:769-82 pubmed publisher
    ..This novel approach demonstrates how mouse models can be used to identify metastasis susceptibility genes, and gives new insight into the molecular mechanisms of fatal PC. ..
  37. Abraham P, Maliekal T. Single cell biology beyond the era of antibodies: relevance, challenges, and promises in biomedical research. Cell Mol Life Sci. 2017;74:1177-1189 pubmed publisher
    ..It is postulated that intracellular molecular markers like nucleostemin (GNL3), alkaline phosphatase (ALPL) and HIRA can be used for improving the outcome of cardiac as well as bone ..
  38. Hua L, Hu B, Yan D, Liu J, Shen Y, Zhao F, et al. Upregulated expression of Nucleostemin/GNL3 is associated with poor prognosis and Sorafenib Resistance in Hepatocellular Carcinoma. Pathol Res Pract. 2017;213:688-697 pubmed publisher
    b>Nucleostemin (NS)/GNL3 protein has been recently documented to be a nucleolar protein that was abundantly expressed in stem cells and cancer cells...
  39. Tang X, Zha L, Li H, Liao G, Huang Z, Peng X, et al. Upregulation of GNL3 expression promotes colon cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway. Oncol Rep. 2017;38:2023-2032 pubmed publisher
    ..In summary, GNL3 is upregulated in colon cancer and plays an important role in tumor growth, invasion and metastasis. Strategies targeting GNL3 are potential treatments for colon cancer. ..
  40. Tanabe E, Kitayoshi M, Fujii K, Ohmori H, Luo Y, Kadochi Y, et al. Fatty acids inhibit anticancer effects of 5-fluorouracil in mouse cancer cell lines. Oncol Lett. 2017;14:681-686 pubmed publisher
    ..The expression of the stem cell markers CD133 and nucleostemin (NS) increased in all three cell lines treated concurrently with 5-FU and either LA or EA when compared with ..
  41. Thoompumkal I, Rehna K, Anbarasu K, Mahalingam S. Leucine Zipper Down-regulated in Cancer-1 (LDOC1) interacts with Guanine nucleotide binding protein-like 3-like (GNL3L) to modulate Nuclear Factor-kappa B (NF-?B) signaling during cell proliferation. Cell Cycle. 2016;15:3251-3267 pubmed
    ..Collectively, our data provides evidence that GNL3L-LDOC1 interplay regulates cell proliferation through the modulation of NF-?B pathway during tumorigenesis. ..
  42. Jia Y, Wei Y, Zhang F, Li Z, Liu S, Yue B. [Influence of Nucleostemin Expression Down-Regulation on PI3K/AKT/mTOR Signal Pathway in HL-60 Cells]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017;25:1592-1596 pubmed publisher
    To explore the effect of nucleostemin(NS) RNAi on the expression of signal molecules in PI3K/AKT/mTOR pathway, a candidate of p53-independent signal pathway in the leukemia HL-60 cells...
  43. Zhao S, Xia Y, Zhang F, Xiong Z, Li Y, Yan W, et al. Nucleostemin dysregulation contributes to ischemic vulnerability of diabetic hearts: Role of ribosomal biogenesis. J Mol Cell Cardiol. 2017;108:106-113 pubmed publisher
    ..b>Nucleostemin (NS) is a nucleolar protein that controls ribosomal biogenesis and exerts cardioprotective effects against I/R ..
  44. Yang J, Zhao Q, Wang K, Liu H, Ma C, Huang H, et al. Isolation and biological characterization of tendon-derived stem cells from fetal bovine. In Vitro Cell Dev Biol Anim. 2016;52:846-56 pubmed publisher
    ..The expressions of stem cell marker nucleostemin and tenocyte-related markers, such as collagen I, collagen II, collagen III, and tenascin-C, were observed on ..
  45. Jeon Y, Park Y, Cho H, Jung H, Ahn T, Kang H, et al. The nucleolar GTPase nucleostemin-like 1 plays a role in plant growth and senescence by modulating ribosome biogenesis. J Exp Bot. 2015;66:6297-310 pubmed publisher
    b>Nucleostemin is a nucleolar GTP-binding protein that is involved in stem cell proliferation, embryonic development, and ribosome biogenesis in mammals...
  46. Hu B, Hua L, Ni W, Wu M, Yan D, Chen Y, et al. Nucleostemin/GNL3 promotes nucleolar polyubiquitylation of p27kip1 to drive hepatocellular carcinoma progression. Cancer Lett. 2017;388:220-229 pubmed publisher
    ..Herein, we revealed that p27 exhibited apparent nucleolar distribution and interacted with nucleolar protein nucleostemin (NS) in Hepatocellular carcinoma (HCC) cells...
  47. Bao Z, Wang Y, Yang L, Wang L, Zhu L, Ban N, et al. Nucleostemin promotes the proliferation of human glioma via Wnt/β-Catenin pathway. Neuropathology. 2016;36:237-49 pubmed publisher
    b>Nucleostemin, nucleolar guanosine triphosphate (GTP)-binding protein 3, is a member of the MMR1/HSR1 GTP-binding protein family...
  48. Gil Ranedo J, Mendiburu Eliçabe M, Garcia Villanueva M, Medina D, del Alamo M, Izquierdo M. An off-target nucleostemin RNAi inhibits growth in human glioblastoma-derived cancer stem cells. PLoS ONE. 2011;6:e28753 pubmed publisher
    ..We hypothesized that nucleostemin may contribute to cancer stem cell biology as these cells share characteristics with normal stem cells...
  49. Trentz O, Arikketh D, Sentilnathan V, Hemmi S, Handschin A, de Rosario B, et al. Surface proteins and osteoblast markers: characterization of human adipose tissue-derived osteogenic cells. Eur J Trauma Emerg Surg. 2010;36:457-63 pubmed publisher
    ..Osteocalcin (OC), core binding factor 1 (CBFA1), collagen type 1 (Coll1), alkaline phosphatase (ALP), nucleostemin (NS), and surface proteins CD 10, CD44, CD 59 and CD 105 were analyzed using RT-PCR, immunofluorescence and ..
  50. Wang Y, DiMario P. Loss of Drosophila nucleostemin 2 (NS2) blocks nucleolar release of the 60S subunit leading to ribosome stress. Chromosoma. 2017;126:375-388 pubmed publisher
    Four nucleostemin-like proteins (nucleostemin (NS) 1-4) were identified previously in Drosophila melanogaster. NS1 and NS2 are nucleolar proteins, while NS3 and NS4 are cytoplasmic proteins...
  51. Manikas R, Thomson E, Thoms M, Hurt E. The K⁺-dependent GTPase Nug1 is implicated in the association of the helicase Dbp10 to the immature peptidyl transferase centre during ribosome maturation. Nucleic Acids Res. 2016;44:1800-12 pubmed publisher
    ..One such enzyme is the conserved circularly permuted GTPase Nug1 (nucleostemin in human)...
  52. Ali M, Naka K, Yoshida A, Fuse K, Kasada A, Hoshii T, et al. Association of a murine leukaemia stem cell gene signature based on nucleostemin promoter activity with prognosis of acute myeloid leukaemia in patients. Biochem Biophys Res Commun. 2014;450:837-43 pubmed publisher
    ..capacity of AML cells fractionated according to the expression of a nucleolar GTP binding protein, nucleostemin (NS)...
  53. Yue B, Yu L, Wang Y. [Effects of silent nucleostemin gene expression on apoptosis of HL-60 cells in vitro]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009;17:319-23 pubmed
    This study was aimed to explore whether the apoptosis of leukemia cells can be induced by targeting silencing nucleostemin gene in vitro...
  54. You Y, Li X, Zheng J, Wu Y, He Y, Du W, et al. Transcript level of nucleostemin in newly diagnosed acute myeloid leukemia patients. Leuk Res. 2013;37:1636-41 pubmed publisher
    To clarify the role of nucleostemin (NS) in AML, its transcription levels in bone marrow (BM) samples obtained from 128 newly diagnosed AML patients were analyzed...
  55. Politz J, Polena I, Trask I, Bazett Jones D, Pederson T. A nonribosomal landscape in the nucleolus revealed by the stem cell protein nucleostemin. Mol Biol Cell. 2005;16:3401-10 pubmed
    b>Nucleostemin is a p53-interactive cell cycle progression factor that shuttles between the nucleolus and nucleoplasm, but it has no known involvement in ribosome synthesis...
  56. Goes F, Hamshere M, Seifuddin F, Pirooznia M, Belmonte Mahon P, Breuer R, et al. Genome-wide association of mood-incongruent psychotic bipolar disorder. Transl Psychiatry. 2012;2:e180 pubmed publisher
    ..The results of our polygenic analysis provides support for a modest degree of genetic overlap between BP with MICP and SZ, highlighting that phenotypic correlations across syndromes may be due to the influence of polygenic risk factors. ..
  57. Kobayashi T, Masutomi K, Tamura K, Moriya T, Yamasaki T, Fujiwara Y, et al. Nucleostemin expression in invasive breast cancer. BMC Cancer. 2014;14:215 pubmed publisher
    ..b>Nucleostemin (NS), initially cloned from rat neural stem cells, binds to various proteins, including p53, in the nucleus and ..
  58. Zia Jahromi N, Hejazi S, Panjepour M, Parivar K, Gharagozloo M. Comparison of nucleostemin gene expression in CD133+ and CD133- cell population in colon cancer cell line HT29. J Cancer Res Ther. 2014;10:68-72 pubmed publisher
    b>Nucleostemin has been shown to be essential for proliferation and survival of colon cancer cells. In this study, we evaluate and comparing nucleostemin expression in CD133+ and CD133- colon cancer cell line HT29...
  59. Gee F, Clubbs C, Raine E, Reynard L, Loughlin J. Allelic expression analysis of the osteoarthritis susceptibility locus that maps to chromosome 3p21 reveals cis-acting eQTLs at GNL3 and SPCS1. BMC Med Genet. 2014;15:53 pubmed publisher
    ..cis-acting regulatory polymorphisms acting on GNL3 and SPCS1 contribute to the OA association signal at chromosome 3p21, and these genes therefore merit further investigation. ..
  60. Asadi M, Derakhshani A, Mowla S. Concomitant upregulation of nucleostemin and downregulation of Sox2 and Klf4 in gastric adenocarcinoma. Tumour Biol. 2014;35:7177-85 pubmed publisher
    b>Nucleostemin (NS) is a nucleolar protein involved in stem cell (SC) self-renewal by controlling cell cycle progression...
  61. Liu R, Zhang Z, Xu Y. Downregulation of nucleostemin causes G1 cell cycle arrest via a p53-independent pathway in prostate cancer PC-3 cells. Urol Int. 2010;85:221-7 pubmed publisher
    To screen the genes and possible signal transduction pathways with which nucleostemin (NS) interacts and explore the mechanism of NS in prostate cancer...
  62. Liu R, Xu Y, Zhang Z, Wang M, Sun J, Zhang Y, et al. [Gene profiling after knocking-down the expression of NS gene in prostate cancer PC-3 cells]. Zhonghua Zhong Liu Za Zhi. 2009;31:561-5 pubmed
    To screen the genes and possible signal transduction pathways involved in the mechanism of nucleostemin (NS) in the proliferation of prostate cancer...
  63. Ali M, Fuse K, Tadokoro Y, Hoshii T, Ueno M, Kobayashi M, et al. Functional dissection of hematopoietic stem cell populations with a stemness-monitoring system based on NS-GFP transgene expression. Sci Rep. 2017;7:11442 pubmed publisher
    ..a transgenic mouse in which green florescence protein (GFP) is driven by the promoter/enhancer region of the nucleostemin (NS) gene...
  64. Carraro A, Trevellin E, Fassan M, Kotsafti A, Lunardi F, Porzionato A, et al. Esophageal adenocarcinoma microenvironment: Peritumoral adipose tissue effects associated with chemoresistance. Cancer Sci. 2017;: pubmed publisher
    ..In patients with EAC, expression of CD34, CD45, CD90 and nucleostemin (NSTM) was higher in peritumoral than in distal adipose tissue and decreased in the presence of neoadjuvant ..
  65. Kawashima M, Kawakita T, Maida Y, Kamoi M, Ogawa Y, Shimmura S, et al. Comparison of telomere length and association with progenitor cell markers in lacrimal gland between Sjögren syndrome and non-Sjögren syndrome dry eye patients. Mol Vis. 2011;17:1397-404 pubmed
    ..Immunostaining for p63, nucleostemin, ATP-binding cassette, sub-family G, member 2 (ABCG2), and nestin was also performed...
  66. Katano M, Ema M, Nakachi Y, Mizuno Y, Hirasaki M, Suzuki A, et al. Forced expression of Nanog or Esrrb preserves the ESC status in the absence of nucleostemin expression. Stem Cells. 2015;33:1089-101 pubmed publisher
    b>Nucleostemin (NS) is a nucleolar GTP-binding protein that is involved in a plethora of functions including ribosomal biogenesis and maintenance of telomere integrity...
  67. Fakhimahmadi A, Nazmi F, Rahmati M, Bonab N, Hashemi M, Moosavi M. Nucleostemin silencing induces differentiation and potentiates all-trans-retinoic acid effects in human acute promyelocytic leukemia NB4 cells via autophagy. Leuk Res. 2017;63:15-21 pubmed publisher
    Here, we report that targeting Nucleostemin (NS), a recently discovered stem cells-enriched gene, by a specific small interference RNA (siNS), decreases the rate of proliferation of acute promyelocytic leukemia (APL) NB4 cells and induces ..
  68. Zhang Q, Zhou X, Wu R, Mosley A, Zeng S, Xing Z, et al. The role of IMP dehydrogenase 2 in Inauhzin-induced ribosomal stress. elife. 2014;3: pubmed publisher
    ..SIRT1, can also inhibit cellular IMPDH2 activity, and reduce the levels of cellular GTP and GTP-binding nucleostemin that is essential for rRNA processing...
  69. Schwartz P, Bryant P, Fuja T, Su H, O Dowd D, Klassen H. Isolation and characterization of neural progenitor cells from post-mortem human cortex. J Neurosci Res. 2003;74:838-51 pubmed
    ..confirmed nestin, SOX2, GFAP, and doublecortin expression and also showed epidermal growth factor receptor and nucleostemin expression during the expansion phase...
  70. Hsu J, Lin T, Tsai R. Nucleostemin prevents telomere damage by promoting PML-IV recruitment to SUMOylated TRF1. J Cell Biol. 2012;197:613-24 pubmed publisher
    ..Here, we report a novel telomere-protecting mechanism regulated by nucleostemin (NS)...
  71. Lin S, Yu H. Clinical significance of nucleostemin expression and its correlation with cyclin D1 expression in malignant ovarian tumors. Int J Gynecol Cancer. 2011;21:1166-71 pubmed publisher
    To investigate the expression of the nucleostemin (NS) gene in ovarian tumors and its correlation with the expression of cyclin D1...
  72. Wang J, Wang L, Ji Q, Zhu H, Han S. Knockdown of Nucleostemin in an ovarian cancer SKOV-3 cell line and its effects on cell malignancy. Biochem Biophys Res Commun. 2017;487:262-267 pubmed publisher
    b>Nucleostemin plays an essential role in the proliferation of some stem cells and cancer cells, but no research has been conducted to assess the link between Nucleostemin and ovarian cancer...
  73. Hariharan N, Quijada P, Mohsin S, Joyo A, Samse K, Monsanto M, et al. Nucleostemin rejuvenates cardiac progenitor cells and antagonizes myocardial aging. J Am Coll Cardiol. 2015;65:133-47 pubmed publisher
    ..b>Nucleostemin (NS) is a nucleolar protein regulating stem cell proliferation and pluripotency...
  74. Lo D, Dai M, Sun X, Zeng S, Lu H. Ubiquitin- and MDM2 E3 ligase-independent proteasomal turnover of nucleostemin in response to GTP depletion. J Biol Chem. 2012;287:10013-20 pubmed publisher
    b>Nucleostemin (NS) is a nucleolar GTP-binding protein essential for ribosomal biogenesis, proliferation, and animal embryogenesis. It remains largely unclear how this protein is regulated...
  75. Lo D, Zhang Y, Dai M, Sun X, Zeng S, Lu H. Nucleostemin stabilizes ARF by inhibiting the ubiquitin ligase ULF. Oncogene. 2015;34:1688-97 pubmed publisher
    Upregulated expression of nucleolar GTPase nucleostemin (NS) has been associated with increased cellular proliferation potential and tumor malignancy during cancer development...
  76. Yuan F, Cheng Q, Li G, Tong T. Nucleostemin Knockdown Sensitizes Hepatocellular Carcinoma Cells to Ultraviolet and Serum Starvation-Induced Apoptosis. PLoS ONE. 2015;10:e0141678 pubmed publisher
    b>Nucleostemin (NS) is a GTP-binding protein that is predominantly expressed in embryonic and adult stem cells but not in terminally differentiated cells...
  77. Zwolinska A, Heagle Whiting A, Beekman C, Sedivy J, Marine J. Suppression of Myc oncogenic activity by nucleostemin haploinsufficiency. Oncogene. 2012;31:3311-21 pubmed publisher
    b>Nucleostemin (NS), a nucleolar GTPase, is highly expressed in stem/progenitor cells and in most cancer cells. However, little is known about the regulation of its expression...
  78. Zhang G, Yin L, Li S, Xing W, Zhao Q, Le X, et al. [Expression of nucleostemin mRNA and protein in the esophageal squamous cell carcinoma]. Zhonghua Zhong Liu Za Zhi. 2008;30:125-8 pubmed
    To investigate the mRNA and protein expression of nucleostemin (NS) in human esophageal squamous cell carcinoma...
  79. Louka M, Zakaria Z, Nagaty M, Elsebaie M, Nabil L. Expression of nucleostemin gene in primary osteoarthritis. Gene. 2016;587:27-32 pubmed publisher
    ..b>Nucleostemin (GNL3) is a nucleolar GTPase initially identified in the nucleolus of rat neural stem cells...
  80. Takagi M, Absalon M, McLure K, Kastan M. Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin. Cell. 2005;123:49-63 pubmed
    ..These findings demonstrate the importance of increased translation of p53 in DNA-damage responses and suggest critical roles for RPL26 and nucleolin in affecting p53 induction. ..
  81. Scott L, Muglia P, Kong X, Guan W, Flickinger M, Upmanyu R, et al. Genome-wide association and meta-analysis of bipolar disorder in individuals of European ancestry. Proc Natl Acad Sci U S A. 2009;106:7501-6 pubmed publisher
    ..37, P = 0.042). Although these results are promising, analysis of additional samples will be required to confirm that variant(s) in these regions influence BP risk. ..
  82. Dastani Z, Hivert M, Timpson N, Perry J, Yuan X, Scott R, et al. Novel loci for adiponectin levels and their influence on type 2 diabetes and metabolic traits: a multi-ethnic meta-analysis of 45,891 individuals. PLoS Genet. 2012;8:e1002607 pubmed publisher
    ..4×10(-4), n = 121,335). These findings identify novel genetic determinants of adiponectin levels, which, taken together, influence risk of T2D and markers of insulin resistance. ..
  83. Liu S, Zhang Z, Zhang D, Sui X, Liu Y, Cai Z, et al. Gene profiling after knocking-down expression of nucleostemin in Hela cells using oligonucleotide DNA microarray. J Exp Clin Cancer Res. 2006;25:575-83 pubmed
    b>Nucleostemin (NS) is preferentially and exclusively expressed in the stem cells and cancer cells, but not in differentiated adult tissues and cells...
  84. Sun Y, Tan X, Tang Z, Peng G, Yang S, Wang R, et al. Knockdown of nucleostemin can inhibit the proliferation of esophageal carcinoma cells in vitro through upregulating p21. Hepatogastroenterology. 2014;61:2247-52 pubmed
    Esophageal squamous cell carcinoma is a common digestive tract cancer with poor prognosis. Nucleostemin is a nucleolar protein expressed in stem and cancer cells, involved in the regulation of cell proliferation...
  85. Huang M, Whang P, Chodaparambil J, Pollyea D, Kusler B, Xu L, et al. Reactive oxygen species regulate nucleostemin oligomerization and protein degradation. J Biol Chem. 2011;286:11035-46 pubmed publisher
    b>Nucleostemin (NS) is a nucleolar-nucleoplasmic shuttle protein that regulates cell proliferation, binds p53 and Mdm2, and is highly expressed in tumor cells...
  86. Höck J, Weinmann L, Ender C, Rüdel S, Kremmer E, Raabe M, et al. Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells. EMBO Rep. 2007;8:1052-60 pubmed
    ..Using reporter assays and knockdown experiments, we showed that the putative RNA-binding protein RBM4 is required for microRNA-guided gene regulation. ..