Gene Symbol: NUBP2
Description: nucleotide binding protein 2
Alias: CFD1, NBP 2, NUBP1, cytosolic Fe-S cluster assembly factor NUBP2, homolog of yeast cytosolic Fe-S cluster deficient 1, nucleotide binding protein 2 (MinD homolog, E. coli)
Species: human
Products:     NUBP2

Top Publications

  1. Freibert S, Goldberg A, Hacker C, Molik S, Dean P, Williams T, et al. Evolutionary conservation and in vitro reconstitution of microsporidian iron-sulfur cluster biosynthesis. Nat Commun. 2017;8:13932 pubmed publisher
    ..The T. hominis cytosolic iron-sulfur protein assembly (CIA) pathway includes the essential Cfd1-Nbp35 scaffold complex that assembles a [4Fe-4S] cluster as shown by spectroscopic methods in vitro...
  2. Grossman J, Camire E, Perlstein D. Approaches to Interrogate the Role of Nucleotide Hydrolysis by Metal Trafficking NTPases: The Nbp35-Cfd1 Iron-Sulfur Cluster Scaffold as a Case Study. Methods Enzymol. 2018;599:293-325 pubmed publisher
    ..activity of the CIA scaffold from yeast, which is comprised of two homologous polypeptides called Nbp35 and Cfd1. In particular, we report two different equilibrium binding assays that make use of commercially available ..
  3. Stehling O, Jeoung J, Freibert S, Paul V, Bänfer S, Niggemeyer B, et al. Function and crystal structure of the dimeric P-loop ATPase CFD1 coordinating an exposed [4Fe-4S] cluster for transfer to apoproteins. Proc Natl Acad Sci U S A. 2018;115:E9085-E9094 pubmed publisher
    ..We show that CFD1, in complex with NBP35 (NUBP1), performs a crucial role in the maturation of all tested cytosolic and nuclear Fe-S proteins, including essential ..
  4. Ververis A, Christodoulou A, Christoforou M, Kamilari C, Lederer C, Santama N. A novel family of katanin-like 2 protein isoforms (KATNAL2), interacting with nucleotide-binding proteins Nubp1 and Nubp2, are key regulators of different MT-based processes in mammalian cells. Cell Mol Life Sci. 2016;73:163-84 pubmed publisher
    ..themselves, with each other and moreover directly and independently with MRP/MinD-type P-loop NTPases Nubp1 and Nubp2, which are integral components of centrioles, negative regulators of ciliogenesis and implicated in centriole ..
  5. Camire E, Grossman J, Thole G, Fleischman N, Perlstein D. The Yeast Nbp35-Cfd1 Cytosolic Iron-Sulfur Cluster Scaffold Is an ATPase. J Biol Chem. 2015;290:23793-802 pubmed publisher
    Nbp35 and Cfd1 are prototypical members of the MRP/Nbp35 class of iron-sulfur (FeS) cluster scaffolds that function to assemble nascent FeS clusters for transfer to FeS-requiring enzymes...
  6. Teumer A, Qi Q, Nethander M, Aschard H, Bandinelli S, Beekman M, et al. Genomewide meta-analysis identifies loci associated with IGF-I and IGFBP-3 levels with impact on age-related traits. Aging Cell. 2016;15:811-24 pubmed publisher
    ..loci associated with circulating IGF-I and IGFBP-3 concentrations (IGF1, IGFBP3, GCKR, TNS3, GHSR, FOXO3, ASXL2, NUBP2/IGFALS, SORCS2, and CELSR2)...
  7. Stehling O, Netz D, Niggemeyer B, Rösser R, Eisenstein R, Puccio H, et al. Human Nbp35 is essential for both cytosolic iron-sulfur protein assembly and iron homeostasis. Mol Cell Biol. 2008;28:5517-28 pubmed publisher
    ..huNbp35 formed a complex with its close homologue huCfd1 (also known as Nubp2) in vivo, suggesting the existence of a heteromeric P-loop NTPase complex that is required for both cytosolic Fe/S ..
  8. Okuno T, Yamabayashi H, Kogure K. Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins. Mol Biol Rep. 2010;37:1165-8 pubmed publisher
    Nubp1 (also known as Nbp35) and Nubp2 (also known as Cfd1) proteins are known to be responsible for regulating centrosome duplication in mouse and ribosome biogenesis in yeast. Nubp proteins contribute to diverse physiological functions...
  9. Netz D, Genau H, Weiler B, Bill E, Pierik A, Lill R. The conserved protein Dre2 uses essential [2Fe-2S] and [4Fe-4S] clusters for its function in cytosolic iron-sulfur protein assembly. Biochem J. 2016;473:2073-85 pubmed publisher
    ..Tah18-Fe-S protein Dre2 that facilitates the de novo assembly of a [4Fe-4S] cluster on the scaffold complex Cfd1-Nbp35...

More Information


  1. Johnson N, Deck K, Nizzi C, Eisenstein R. A synergistic role of IRP1 and FBXL5 proteins in coordinating iron metabolism during cell proliferation. J Biol Chem. 2017;292:15976-15989 pubmed publisher
    ..mutant (IRP13C>3S) that cannot insert the Fe-S cluster, or along with knockdown of the CIA factors NUBP2 or FAM96A, reduced cell viability...
  2. Gress T, Müller Pillasch F, Geng M, Zimmerhackl F, Zehetner G, Friess H, et al. A pancreatic cancer-specific expression profile. Oncogene. 1996;13:1819-30 pubmed
    ..In addition this has led to the identification of novel differentially expressed genes, which represent potential oncogenes or disease-associated markers and may be helpful for the development of therapeutic or diagnostic modalities. ..
  3. Nakashima H, Grahovac M, Mazzarella R, Fujiwara H, Kitchen J, Threat T, et al. Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome 17, and Nubp1, mapped to chromosome 16--establish a new gene family of nucleotide-binding proteins in eukaryotes. Genomics. 1999;60:152-60 pubmed
    ..Phylogenetic analyses of the genes, encoding a short form (Nubp2) and a long form (Nubp1) of NUBP, clearly establish them as a new NUBP/MRP gene family that is well conserved ..
  4. Roy A, Solodovnikova N, Nicholson T, Antholine W, Walden W. A novel eukaryotic factor for cytosolic Fe-S cluster assembly. EMBO J. 2003;22:4826-35 pubmed
    ..of IRP1 to c-aconitase led to the identification of a previously uncharacterized, essential gene, which we call CFD1 (cytosolic Fe-S cluster deficient). CFD1 encodes a highly conserved, putative P-loop ATPase...
  5. Yarunin A, Panse V, Petfalski E, Dez C, Tollervey D, Hurt E. Functional link between ribosome formation and biogenesis of iron-sulfur proteins. EMBO J. 2005;24:580-8 pubmed
    In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis...
  6. Kaplan R, Petersen A, Chen M, Teumer A, Glazer N, Doring A, et al. A genome-wide association study identifies novel loci associated with circulating IGF-I and IGFBP-3. Hum Mol Genet. 2011;20:1241-51 pubmed publisher
    ..These genetic loci deserve further investigation to elucidate the biological basis for the observed associations and clarify their possible role in IGF-mediated regulation of cell growth and metabolism. ..
  7. Bastow E, Bych K, Crack J, Le Brun N, Balk J. NBP35 interacts with DRE2 in the maturation of cytosolic iron-sulphur proteins in Arabidopsis thaliana. Plant J. 2017;89:590-600 pubmed publisher
    ..of the cytosolic pathway for iron-sulphur (FeS) cluster assembly are conserved, except that plants lack a gene for CFD1 (Cytosolic FeS cluster Deficient 1)...