NUB1

Summary

Gene Symbol: NUB1
Description: negative regulator of ubiquitin like proteins 1
Alias: BS4, NUB1L, NYREN18, NEDD8 ultimate buster 1, NEDD8 ultimate buster-1, renal carcinoma antigen NY-REN-18
Species: human
Products:     NUB1

Top Publications

  1. Schmidtke G, Kalveram B, Weber E, Bochtler P, Lukasiak S, Hipp M, et al. The UBA domains of NUB1L are required for binding but not for accelerated degradation of the ubiquitin-like modifier FAT10. J Biol Chem. 2006;281:20045-54 pubmed
    ..NEDD8 ultimate buster-1 long (NUB1L) belongs to this class of proteins and contains one UBL and three UBA domains...
  2. Hidalgo de Quintana J, Evans R, Cheetham M, van der Spuy J. The Leber congenital amaurosis protein AIPL1 functions as part of a chaperone heterocomplex. Invest Ophthalmol Vis Sci. 2008;49:2878-87 pubmed publisher
    ..TPR) cochaperones that interact with the chaperone Hsp90 and the ability of AIPL1 to suppress the aggregation of NUB1 fragments in a chaperone-like manner suggest that AIPL1 might function as part of a chaperone heterocomplex ..
  3. van der Spuy J, Cheetham M. The Leber congenital amaurosis protein AIPL1 modulates the nuclear translocation of NUB1 and suppresses inclusion formation by NUB1 fragments. J Biol Chem. 2004;279:48038-47 pubmed
    ..AIPL1 interacts with the cell cycle regulator NEDD8 ultimate buster protein 1 (NUB1)...
  4. Tanji K, Tanaka T, Mori F, Kito K, Takahashi H, Wakabayashi K, et al. NUB1 suppresses the formation of Lewy body-like inclusions by proteasomal degradation of synphilin-1. Am J Pathol. 2006;169:553-65 pubmed
    b>NUB1 is a potent down-regulator of the ubiquitin-like protein NEDD8, because it targets NEDD8 to the proteasome for proteolytic degradation...
  5. Kito K, Yeh E, Kamitani T. NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression. J Biol Chem. 2001;276:20603-9 pubmed
    ..Here, we report this novel regulator, NUB1 (NEDD8 Ultimate Buster-1). NUB1 is composed of 601 residues with a calculated 69.1-kDa molecular mass...
  6. Kanaya K, Sohocki M, Kamitani T. Abolished interaction of NUB1 with mutant AIPL1 involved in Leber congenital amaurosis. Biochem Biophys Res Commun. 2004;317:768-73 pubmed
    ..Although the function of AIPL1 is unknown, it has been reported to interact with NUB1. Here, we searched for a NUB1-binding site on AIPL1 and located it between amino acid residues 181 and 330 in AIPL1...
  7. van der Spuy J, Kim J, Yu Y, Szel A, Luthert P, Clark B, et al. The expression of the Leber congenital amaurosis protein AIPL1 coincides with rod and cone photoreceptor development. Invest Ophthalmol Vis Sci. 2003;44:5396-403 pubmed
    ..In addition, the expression of NUB1, a putative AIPL1-interacting partner, was examined...
  8. van der Spuy J, Munro P, Luthert P, Preising M, Bek T, Heegaard S, et al. Predominant rod photoreceptor degeneration in Leber congenital amaurosis. Mol Vis. 2005;11:542-53 pubmed
    ..was performed and the consequences of the H82Y amino acid substitution characterized in an in vitro assay of NUB1 modulation. The morphology of the photoreceptors was examined by light and electron microscopy...
  9. Kamitani T, Kito K, Fukuda Kamitani T, Yeh E. Targeting of NEDD8 and its conjugates for proteasomal degradation by NUB1. J Biol Chem. 2001;276:46655-60 pubmed
    ..Recently, we identified a negative regulator of the NEDD8 conjugation system, NUB1, which interacts with NEDD8 and down-regulates NEDD8 expression post-transcriptionally (Kito, K., Yeh, E. T. H...

More Information

Publications47

  1. Schmidtke G, Kalveram B, Groettrup M. Degradation of FAT10 by the 26S proteasome is independent of ubiquitylation but relies on NUB1L. FEBS Lett. 2009;583:591-4 pubmed publisher
    ..targets proteins for degradation by the proteasome, a process accelerated by the UBL-UBA domain protein NEDD8 ultimate buster 1-long...
  2. Tanaka T, Kawashima H, Yeh E, Kamitani T. Regulation of the NEDD8 conjugation system by a splicing variant, NUB1L. J Biol Chem. 2003;278:32905-13 pubmed
    ..Recently, we found its splicing variant, NUB1L. It possesses an insertion of 14 amino acids that codes for a UBA domain...
  3. Hipp M, Raasi S, Groettrup M, Schmidtke G. NEDD8 ultimate buster-1L interacts with the ubiquitin-like protein FAT10 and accelerates its degradation. J Biol Chem. 2004;279:16503-10 pubmed
    ..A yeast two-hybrid screen identified NEDD8 ultimate buster-1L (NUB1L) as a non-covalent binding partner of FAT10, and this interaction was confirmed by coimmunoprecipitation and ..
  4. Akey D, Zhu X, Dyer M, Li A, Sorensen A, Blackshaw S, et al. The inherited blindness associated protein AIPL1 interacts with the cell cycle regulator protein NUB1. Hum Mol Genet. 2002;11:2723-33 pubmed
    ..One of the identified interacting proteins corresponds to NUB1 (NEDD8 Ultimate Buster 1), which is thought to control many biological events, especially cell cycle progression, by ..
  5. Le Niculescu H, ROSEBERRY K, Levey D, Rogers J, Kosary K, Prabha S, et al. Towards precision medicine for stress disorders: diagnostic biomarkers and targeted drugs. Mol Psychiatry. 2019;: pubmed publisher
    ..biological marker of psychological stress and show that newly identified predictive biomarkers such as NUB1, APOL3, MAD1L1, or NKTR are comparable or better state or trait predictors of stress than TL or FKBP5...
  6. Zaineldin A, Hegazi S, Koshio S, Ishikawa M, Bakr A, El Keredy A, et al. Bacillus subtilis as probiotic candidate for red sea bream: Growth performance, oxidative status, and immune response traits. Fish Shellfish Immunol. 2018;79:303-312 pubmed publisher
    ..0 (BS0), 1?×?104 (BS1), 1?×?106 (BS2), 1?×?108 (BS3) and 1?×?1010 (BS4) CFU kg-1 diet for 60 days. The obtained results showed that B...
  7. Tanaka T, Nakatani T, Kamitani T. Negative regulation of NEDD8 conjugation pathway by novel molecules and agents for anticancer therapy. Curr Pharm Des. 2013;19:4131-9 pubmed
    ..g. COP9 signalosome, CAND1, inactive mutant of Ubc12 and NUB1/NUB1L) and clarifies possible strategies for targeting the NEDD8 cascade in cancer cells.
  8. Dampier W, Sullivan N, Mell J, Pirrone V, Ehrlich G, Chung C, et al. Broad spectrum and personalized gRNAs for CRISPR/Cas9 HIV-1 therapeutics. AIDS Res Hum Retroviruses. 2018;: pubmed publisher
    ..design was predicted to cut 46 of 48 patient samples at 90% efficiency, whereas the top 4 broad-spectrum gRNAs (BS4) were predicted to excise provirus from 44 of 48 patient samples with 90% efficiency...
  9. Gross P, Kirchhain A, Höppe H. The Borosulfates K4[BS4O15(OH)], Ba[B2S3O13], and Gd2 [B2S6O24]. Angew Chem Int Ed Engl. 2016;55:4353-5 pubmed publisher
    K4 [BS4 O15 (OH)], Ba[B2 S3 O13 ], and Gd2 [B2 S6 O24 ] were obtained by a new synthetic approach...
  10. Cabral L, Holland B. Courtship song does not increase the rate of adaptation to a thermally stressful environment in a Drosophila melanogaster laboratory population. PLoS ONE. 2014;9:e111148 pubmed publisher
    ..We used non-displaying, outbred populations of D. melanogaster (nub1) mutants and measured their rate of adaptation to a new, thermally stressful environment, relative to wild-type ..
  11. Zanotti L, Romani C, Tassone L, Todeschini P, Tassi R, Bandiera E, et al. MAL gene overexpression as a marker of high-grade serous ovarian carcinoma stem-like cells that predicts chemoresistance and poor prognosis. BMC Cancer. 2017;17:366 pubmed publisher
    ..We isolated a population of cells with stem-like characteristics (OVA-BS4 spheroids) from a primary human EOC cell line under selective conditions...
  12. Tanji K, Miki Y, Maruyama A, Mori F, Mimura J, Itoh K, et al. The role of NUB1 in α-synuclein degradation in Lewy body disease model mice. Biochem Biophys Res Commun. 2016;470:635-642 pubmed publisher
    ..Previously we have shown that NUB1 is accumulated in these specific regions together with abnormal α-synuclein and that NUB1 is able to inhibit α..
  13. Vodicka P, Chase K, Iuliano M, Valentine D, Sapp E, Lu B, et al. Effects of Exogenous NUB1 Expression in the Striatum of HDQ175/Q7 Mice. J Huntingtons Dis. 2016;5:163-74 pubmed publisher
    Reducing mutant huntingtin (mHTT) in neurons may be a therapy for Huntington's disease (HD). Elevating NUB1 protein reduced mHTT levels in cell and fly models of HD through a proteasome dependent mechanism...
  14. Dantoft W, Lundin D, Esfahani S, Engstrom Y. The POU/Oct Transcription Factor Pdm1/nub Is Necessary for a Beneficial Gut Microbiota and Normal Lifespan of Drosophila. J Innate Immun. 2016;8:412-26 pubmed publisher
    ..In addition, the microbial composition shifted considerably during the short lifespan of the nub1 mutants...
  15. Triplett L, Cohen S, Heffelfinger C, Schmidt C, Huerta A, Tekete C, et al. A resistance locus in the American heirloom rice variety Carolina Gold Select is triggered by TAL effectors with diverse predicted targets and is effective against African strains of Xanthomonas oryzae pv. oryzicola. Plant J. 2016;87:472-83 pubmed publisher
    ..between the TAL effector-triggered resistance conferred by Xo1 and that conferred by the tomato resistance gene Bs4 suggests that monocots and dicots share an ancient or convergently evolved mechanism to recognize analogous TAL ..
  16. Wei F, Cai C, Yu P, Lv J, Ling C, Shi W, et al. Quantitative candidate gene association studies of metabolic traits in Han Chinese type 2 diabetes patients. Genet Mol Res. 2015;14:15471-81 pubmed publisher
    ..traits; ABCG2, SLC2A9, and PKHD1 polymorphisms were associated with SUA; CAMTA1, VHL, KAT2B, PON1, NUB1, SLITRK5, SMAD3, FTO, FANCA, and PCSK2 polymorphisms were associated with blood lipid traits; CAMTA1, SPAG16, TOX, ..
  17. Tanaka T, Yeh E, Kamitani T. NUB1-mediated targeting of the ubiquitin precursor UbC1 for its C-terminal hydrolysis. Eur J Biochem. 2004;271:972-82 pubmed
    ..Previously, we identified a negative regulator of the NEDD8 conjugation system, NEDD8 ultimate buster-1 (NUB1), that recruits NEDD8 and its conjugates to the proteasome for degradation...
  18. Hosono T, Tanaka T, Tanji K, Nakatani T, Kamitani T. NUB1, an interferon-inducible protein, mediates anti-proliferative actions and apoptosis in renal cell carcinoma cells through cell-cycle regulation. Br J Cancer. 2010;102:873-82 pubmed publisher
    b>NEDD8 ultimate buster 1 (NUB1) is an interferon (IFN)-inducible protein that downregulates NEDD8 expression and its conjugation system...
  19. Nakayasu E, Ansong C, Brown J, Yang F, Lopez Ferrer D, Qian W, et al. Evaluation of selected binding domains for the analysis of ubiquitinated proteomes. J Am Soc Mass Spectrom. 2013;24:1214-23 pubmed publisher
    ..This approach is especially promising for the analysis of tissues or cells resistant to transfection, of which the overexpression of tagged ubiquitin is a major hurdle. ..
  20. Tanji K, Tanaka T, Kamitani T. Interaction of NUB1 with the proteasome subunit S5a. Biochem Biophys Res Commun. 2005;337:116-20 pubmed
    b>NUB1 interacts with a ubiquitin-like protein NEDD8 to target the NEDD8 monomer and neddylated proteins to the proteasome for degradation. Therefore, NUB1 is thought to be a potent downregulator of NEDD8 conjugation system...
  21. Richet E, Pooler A, Rodriguez T, Novoselov S, Schmidtke G, Groettrup M, et al. NUB1 modulation of GSK3? reduces tau aggregation. Hum Mol Genet. 2012;21:5254-67 pubmed publisher
    ..Here, we examined the role of the proteasome-associated protein Nedd8 ultimate buster 1 (NUB1) in the neuropathogenic phosphorylation and aggregation of tau...
  22. Liu G, Xirodimas D. NUB1 promotes cytoplasmic localization of p53 through cooperation of the NEDD8 and ubiquitin pathways. Oncogene. 2010;29:2252-61 pubmed publisher
    ..In this study we show that NEDD8 ultimate buster 1 (NUB1), a non-covalent interactor of the Ubl NEDD8 (neural precursor cell expressed, developmentally ..
  23. Lu B, Al Ramahi I, Valencia A, Wang Q, Berenshteyn F, Yang H, et al. Identification of NUB1 as a suppressor of mutant Huntington toxicity via enhanced protein clearance. Nat Neurosci. 2013;16:562-70 pubmed publisher
    ..Among these, negative regulator of ubiquitin-like protein 1 (NUB1) overexpression lowered mHTT in neuronal models and rescued mHTT-induced death...
  24. Norman J, Shiekhattar R. Analysis of Nedd8-associated polypeptides: a model for deciphering the pathway for ubiquitin-like modifications. Biochemistry. 2006;45:3014-9 pubmed
    ..of enzymes and complexes known to regulate neddylation and deneddylation, including the COP9 signalosome, Nub1, and enzymes in the neddylation cascade...
  25. Chowdhury R, Bois P, Feingold E, Sherman S, Cheung V. Genetic analysis of variation in human meiotic recombination. PLoS Genet. 2009;5:e1000648 pubmed publisher
    ..Of the four newly identified loci (KIAA1462, PDZK1, UGCG, NUB1), results from expression studies provide support for their roles in meiosis...
  26. Liu S, Fu Q, Zhao J, Hu H. Structural and mechanistic insights into the arginine/lysine-rich peptide motifs that interact with P97/VCP. Biochim Biophys Acta. 2013;1834:2672-8 pubmed publisher
    ..Based on biochemical features of the VBM motifs, we also identified NUB1L (NEDD8 ultimate buster-1 long) as a novel VBM-containing protein, which is involved in proteasomal degradation of ..
  27. Rani N, Aichem A, Schmidtke G, Kreft S, Groettrup M. FAT10 and NUB1L bind to the VWA domain of Rpn10 and Rpn1 to enable proteasome-mediated proteolysis. Nat Commun. 2012;3:749 pubmed publisher
    ..is strongly accelerated by the ubiquitin-like-ubiquitin-associated protein NEDD8 ultimate buster-1 long (NUB1L). Here we show how FAT10 and NUB1L dock with the 26S proteasome to initiate proteolysis...
  28. Jeong Y, Her J, Chung I. NEDD8 ultimate buster-1 regulates the abundance of TRF1 at telomeres by promoting its proteasomal degradation. FEBS Lett. 2016;590:1776-90 pubmed publisher
    ..Here, we describe a novel function of NEDD8 ultimate buster-1 (NUB1) for regulating the levels of TRF1 at telomeres...
  29. Bonacci T, Audebert S, Camoin L, Baudelet E, Iovanna J, Soubeyran P. Regulation of NUB1 Activity through Non-Proteolytic Mdm2-Mediated Ubiquitination. PLoS ONE. 2017;12:e0169988 pubmed publisher
    NUB1 (Nedd8 ultimate buster 1) is an adaptor protein which negatively regulates the ubiquitin-like protein Nedd8 as well as neddylated proteins levels through proteasomal degradation...
  30. Mori F, Tanji K, Odagiri S, Hattori M, Hoshikawa Y, Kono C, et al. Ubiquitin-related proteins in neuronal and glial intranuclear inclusions in intranuclear inclusion body disease. Pathol Int. 2012;62:407-11 pubmed publisher
    ..same sections with hematoxylin and eosin and by immunolabeling with antibodies against ubiquitin and URP (NEDD8, NUB1, SUMO-1 and SUMO-2). Intranuclear inclusions were widely distributed in neurons and glial cells in all the cases...
  31. Theng S, Wang W, Mah W, Chan C, Zhuo J, Gao Y, et al. Disruption of FAT10-MAD2 binding inhibits tumor progression. Proc Natl Acad Sci U S A. 2014;111:E5282-91 pubmed publisher
    ..Our results strongly suggest that the interaction of FAT10 with MAD2 is a key mechanism underlying the promalignant property of FAT10 and offer prospects for the development of anticancer strategies. ..
  32. Tanji K, Mori F, Kito K, Kakita A, Mimura J, Itoh K, et al. Synphilin-1-binding protein NUB1 is colocalized with nonfibrillar, proteinase K-resistant ?-synuclein in presynapses in Lewy body disease. J Neuropathol Exp Neurol. 2011;70:879-89 pubmed publisher
    ..b>NUB1, a synphilin-1-binding protein, also accumulates in Lewy bodies, but it is not known whether abnormal ?-synuclein ..
  33. Rodríguez Morgado B, Caballero P, Paneque P, Gómez I, Parrado J, Tejada M. Obtaining edaphic biostimulants/biofertilizers from sewage sludge using fermentative processes. Short-time effects on soil biochemical properties. Environ Technol. 2017;:1-8 pubmed publisher
    ..Only dehydrogenase activity and ergosterol content were significantly stimulated after the application of BS1 and BS4. Rest of the extracellular activities were not stimulated probably because B...
  34. Li J, Ma W, Li H, Hou N, Wang X, Kim I, et al. NEDD8 Ultimate Buster 1 Long (NUB1L) Protein Suppresses Atypical Neddylation and Promotes the Proteasomal Degradation of Misfolded Proteins. J Biol Chem. 2015;290:23850-62 pubmed publisher
    ..Proteotoxic stresses induced typical and atypical neddylation in cardiomyocytes. Loss of NUB1L exaggerated atypical neddylation, whereas NUB1L overexpression repressed atypical neddylation through promoting ..
  35. Liu S, Yang H, Zhao J, Zhang Y, Song A, Hu H. NEDD8 ultimate buster-1 long (NUB1L) protein promotes transfer of NEDD8 to proteasome for degradation through the P97UFD1/NPL4 complex. J Biol Chem. 2013;288:31339-49 pubmed publisher
    The NEDD8 protein and neddylation levels in cells are modulated by NUB1L or NUB1 through proteasomal degradation, but the underlying molecular mechanism is not well understood...
  36. Tanji K, Mori F, Kakita A, Zhang H, Kito K, Kamitani T, et al. Immunohistochemical localization of NUB1, a synphilin-1-binding protein, in neurodegenerative disorders. Acta Neuropathol. 2007;114:365-71 pubmed
    Recently, we showed that NUB1 is a synphilin-1-interacting protein and that NUB1, as well as synphilin-1, accumulates in Lewy bodies in Parkinson's disease (PD) and dementia with Lewy bodies (DLB), and glial cytoplasmic inclusions in ..
  37. Bett J, Kanuga N, Richet E, Schmidtke G, Groettrup M, Cheetham M, et al. The inherited blindness protein AIPL1 regulates the ubiquitin-like FAT10 pathway. PLoS ONE. 2012;7:e30866 pubmed publisher
    ..AIPL1 has previously been shown to interact with NUB1, which facilitates the proteasomal degradation of proteins modified with the ubiquitin-like protein FAT10...
  38. Akey D, Zhu X, Dyer M, Li A, Sorensen A, Fukada Kamitani T, et al. Functional studies of AIPL1: potential role of AIPL1 in cell cycle exit and/or differentiation of photoreceptors. Adv Exp Med Biol. 2003;533:287-95 pubmed