NFS1

Summary

Gene Symbol: NFS1
Description: NFS1, cysteine desulfurase
Alias: HUSSY-08, IscS, NIFS, cysteine desulfurase, mitochondrial, NFS1 nitrogen fixation 1 homolog, nitrogen fixation 1 (S. cerevisiae, homolog), nitrogen-fixing bacteria S-like protein
Species: human
Products:     NFS1

Top Publications

  1. Land T, Rouault T. Targeting of a human iron-sulfur cluster assembly enzyme, nifs, to different subcellular compartments is regulated through alternative AUG utilization. Mol Cell. 1998;2:807-15 pubmed
    ..Here we report cloning of the human homolog of NifS, a cysteine desulfurase that is proposed to supply the inorganic sulfur in iron-sulfur clusters...
  2. Tong W, Rouault T. Distinct iron-sulfur cluster assembly complexes exist in the cytosol and mitochondria of human cells. EMBO J. 2000;19:5692-700 pubmed
    ..Biochemical analyses demonstrate that IscU proteins specifically associate with IscS, a cysteine desulfurase that is proposed to sequester inorganic sulfur for Fe-S cluster assembly...
  3. Marelja Z, Stöcklein W, Nimtz M, Leimkuhler S. A novel role for human Nfs1 in the cytoplasm: Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis. J Biol Chem. 2008;283:25178-85 pubmed publisher
    ..In this report, we investigated the involvement of the human L-cysteine desulfurase Nfs1 in sulfur transfer to MOCS3-RLD...
  4. Naamati A, Regev Rudzki N, Galperin S, Lill R, Pines O. Dual targeting of Nfs1 and discovery of its novel processing enzyme, Icp55. J Biol Chem. 2009;284:30200-8 pubmed publisher
    ..In eukaryotes, each subcellular compartment harbors a specific group of proteins that must accomplish specific tasks. Nfs1 is a highly conserved mitochondrial cysteine desulfurase that participates in iron-sulfur cluster assembly as a ..
  5. Biederbick A, Stehling O, Rösser R, Niggemeyer B, Nakai Y, Elsässer H, et al. Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein biogenesis and iron regulation. Mol Cell Biol. 2006;26:5675-87 pubmed
    ..Here, we have analyzed the role of the human cysteine desulfurase Nfs1 (huNfs1), which serves as a sulfur donor in biogenesis...
  6. Tsai C, Barondeau D. Human frataxin is an allosteric switch that activates the Fe-S cluster biosynthetic complex. Biochemistry. 2010;49:9132-9 pubmed publisher
    ..Here we provide in vitro evidence that human frataxin binds to a Nfs1, Isd11, and Isu2 complex to generate the four-component core machinery for Fe-S cluster biosynthesis...
  7. Caballero Molada M, Planes M, Benlloch H, Atares S, Naranjo M, Serrano R. The Gcn2-eIF2α pathway connects iron and amino acid homeostasis in Saccharomyces cerevisiae. Biochem J. 2018;475:1523-1534 pubmed publisher
    ..As several enzymes of amino acid biosynthesis contain iron-sulfur clusters (ISCs) and iron excess is toxic, iron and amino acid homeostasis should be co-ordinated...
  8. Kriz V, Korinek V. Wnt, RSPO and Hippo Signalling in the Intestine and Intestinal Stem Cells. Genes (Basel). 2018;9: pubmed publisher
    ..In intestinal stem cells (ISCs), the Wnt pathway is essential for intestinal crypt formation and renewal, whereas RSPO-mediated signalling mainly ..
  9. Thakkar U, Trivedi H, Vanikar A, Dave S. Insulin-secreting adipose-derived mesenchymal stromal cells with bone marrow-derived hematopoietic stem cells from autologous and allogenic sources for type 1 diabetes mellitus. Cytotherapy. 2015;17:940-7 pubmed publisher
    ..Group 1 received mean SCs 103.14 mL with 2.65 ± 0.8 × 10(4) ISCs/kg body wt, CD34+ 0.81% and CD45-/90+/73+, 81.55%. Group 2 received mean SCs 95.33 mL with 2.07 ± 0...

More Information

Publications131 found, 100 shown here

  1. Liu Y, Wang Y, Chakroff J, Johnson J, Farrell A, Besner G. Production of Tissue-Engineered Small Intestine in Rats with Different Ages of Cell Donors. Tissue Eng Part A. 2018;: pubmed publisher
    ..Gene expression for Lgr5 (indicative of actively dividing intestinal stem cells (ISCs)) and for Bmi1 and Hopx (indicative of quiescent stem cells) was higher in younger donor intestines...
  2. Wong H, Qin H, Tsang S, Zuo X, Che S, Chow C, et al. Early life stress disrupts intestinal homeostasis via NGF-TrkA signaling. Nat Commun. 2019;10:1745 pubmed publisher
    ..Here we show that NMS induces expansion of intestinal stem cells (ISCs) and their differentiation toward secretory lineages including enterochromaffin (EC) and Paneth cells, leading to ..
  3. Leite F, Reinhardt T, Bannantine J, Stabel J. Envelope protein complexes of Mycobacterium avium subsp. paratuberculosis and their antigenicity. Vet Microbiol. 2015;175:275-85 pubmed publisher
    ..Furthermore the identification of antigenic proteins with potential as diagnostic targets was characterized. ..
  4. Khalil H, Lei N, Brinkley G, Scott A, Wang J, Kar U, et al. A novel culture system for adult porcine intestinal crypts. Cell Tissue Res. 2016;365:123-34 pubmed publisher
    ..Whereas techniques for the culture and genetic manipulation of ISCs from mice and humans are well established, similar methods for porcine stem cells have not been reported...
  5. Malaterre J, Pereira L, Putoczki T, Millen R, Paquet Fifield S, Germann M, et al. Intestinal-specific activatable Myb initiates colon tumorigenesis in mice. Oncogene. 2016;35:2475-84 pubmed publisher
    ..We confirmed that the transgene was faithfully expressed and inducible in intestinal stem cells (ISCs) before embarking on carcinogenesis studies...
  6. Misslinger M, Lechner B, Bacher K, Haas H. Iron-sensing is governed by mitochondrial, not by cytosolic iron-sulfur cluster biogenesis in Aspergillus fumigatus. Metallomics. 2018;10:1687-1700 pubmed publisher
    ..These studies revealed that: (i) Nfs1 (Afu3g14240) and Nbp35 (Afu2g15960), which are involved in ISC and CIA, respectively, are essential for growth; (ii)..
  7. Tan G, Yang J, Li T, Zhao J, Sun S, Li X, et al. Anaerobic Copper Toxicity and Iron-Sulfur Cluster Biogenesis in Escherichia coli. Appl Environ Microbiol. 2017;83: pubmed publisher
    ..The results from this study illustrate a new interplay between intracellular copper toxicity and iron-sulfur cluster biogenesis in bacterial cells under anaerobic conditions. ..
  8. Tao W, Yurkovich M, Wen S, Lebe K, Samborskyy M, Yang A, et al. A genomics-led approach to deciphering the mechanism of thiotetronate antibiotic biosynthesis. Chem Sci. 2016;7:376-385 pubmed publisher
    ..These insights have allowed us to propose a mechanism for sulfur insertion, and have opened the way to engineering of the biosynthesis of TLM and other thiotetronates to produce novel analogues. ..
  9. Yang W, Sun Z, Yang B, Wang Q. Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays. Int J Mol Sci. 2017;18: pubmed publisher
    ..mice, Nrf2 knockout increased the number of Lgr5⁺ intestinal stem cells (ISCs) and their daughter cells including Ki67⁺ transient amplifying cells, Villin⁺ enterocytes, and lysozymeâ..
  10. Moore M, Wofford J, Dancis A, Lindahl P. Recovery of mrs3?mrs4? Saccharomyces cerevisiae cells under Iron-Sufficient Conditions, and the Role of Fe580. Biochemistry. 2017;: pubmed publisher
    ..Mitochondria from respiring ?? cells grown under iron-deficient conditions were devoid of Fe580, ISCs, and hemes; most iron was present as FeIII nanoparticles...
  11. Singh S, Mishra M, Kango Singh M, Hou S. Generation and staining of intestinal stem cell lineage in adult midgut. Methods Mol Biol. 2012;879:47-69 pubmed publisher
    ..Recently, using lineage tracing and molecular marker labeling, intestinal stem cells (ISCs) have been identified in Drosophila adult midgut...
  12. Kishida K, Pearce S, Yu S, Gao N, Ferraris R. Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells. Am J Physiol Gastrointest Liver Physiol. 2017;312:G592-G605 pubmed publisher
    ..Using an innovative approach, we discovered that nutrient sensing is likely repressed in progenitor ISCs then irreversibly derepressed during specification into sensing-competent absorptive or secretory lineages, the ..
  13. Abeti R, Uzun E, Renganathan I, Honda T, Pook M, Giunti P. Targeting lipid peroxidation and mitochondrial imbalance in Friedreich's ataxia. Pharmacol Res. 2015;99:344-50 pubmed publisher
    ..This protein is located in the mitochondria, where it functions in the biogenesis of iron-sulphur clusters (ISCs), which are important for the function of the mitochondrial respiratory chain complexes...
  14. Lo Y, Noah T, Chen M, ZOU W, Borràs E, Vilar E, et al. SPDEF Induces Quiescence of Colorectal Cancer Cells by Changing the Transcriptional Targets of β-catenin. Gastroenterology. 2017;153:205-218.e8 pubmed publisher
    ..TRE-Spdef mice, which express an oncogenic form of β-catenin in Lgr5-positive ISCs upon administration of tamoxifen and SPDEF upon administration of tetracycline...
  15. Rouch J, Scott A, Lei N, Solorzano Vargas R, Wang J, Hanson E, et al. Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids. PLoS ONE. 2016;11:e0148216 pubmed publisher
    ..Recent studies in mice have demonstrated that M cells are generated from Lgr5+ intestinal stem cells (ISCs), and that infection with Salmonella enterica serovar Typhimurium increases M cell formation...
  16. Smith N, Swain J, Davies P, Gallagher A, Parappilly M, Beach C, et al. Monoclonal Antibodies Reveal Dynamic Plasticity Between Lgr5- and Bmi1-Expressing Intestinal Cell Populations. Cell Mol Gastroenterol Hepatol. 2018;6:79-96 pubmed publisher
    ..Growth from singly isolated Lgr5GFP ISCs gave rise to small spheroids...
  17. Roche K, Gracz A, Liu X, Newton V, Akiyama H, Magness S. SOX9 maintains reserve stem cells and preserves radioresistance in mouse small intestine. Gastroenterology. 2015;149:1553-1563.e10 pubmed publisher
    ..Expression of additional markers associated with active and reserve ISCs were assessed in Lgr5(high) and Sox9(high) populations by single-cell gene expression analyses...
  18. Paul A, Drecourt A, Petit F, Deguine D, Vasnier C, Oufadem M, et al. FDXR Mutations Cause Sensorial Neuropathies and Expand the Spectrum of Mitochondrial Fe-S-Synthesis Diseases. Am J Hum Genet. 2017;101:630-637 pubmed publisher
    ..ferredoxin reductase, the sole human ferredoxin reductase implicated in the biosynthesis of iron-sulfur clusters (ISCs) and in heme formation...
  19. Roret T, Pégeot H, Couturier J, Mulliert G, Rouhier N, Didierjean C. X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana. Acta Crystallogr F Struct Biol Commun. 2014;70:1180-5 pubmed publisher
    ..Based on available homologous bacterial complexes, a model of a complex between AtNfs2 and the SufE domain of its biological partner AtSufE1 is proposed, revealing the nature of the binding sites. ..
  20. Park J, Na H, Pyo J, Jeon H, Kim Y, Yoo M. Requirement of ATR for maintenance of intestinal stem cells in aging Drosophila. Aging (Albany NY). 2015;7:307-18 pubmed
    ..telangiectasia-mutated (ATM) and ATM- and RAD3-related (ATR) kinases, in the maintenance of intestinal stem cells (ISCs) in the adultDrosophila midgut...
  21. Yang S, Wang Q, Fedorova E, Liu J, Qin Q, Zheng Q, et al. Microsymbiont discrimination mediated by a host-secreted peptide in Medicago truncatula. Proc Natl Acad Sci U S A. 2017;114:6848-6853 pubmed publisher
    ..Host genetic control of this specificity is unknown. We herein report the cloning of the Medicago truncatula NFS1 gene that regulates the fixation-level incompatibility with the microsymbiont Sinorhizobium meliloti ..
  22. Jones J, Brindley C, Elder N, Myers M, Rajala M, Dekaney C, et al. Cellular Plasticity of Defa4Cre-Expressing Paneth Cells in Response to Notch Activation and Intestinal Injury. Cell Mol Gastroenterol Hepatol. 2019;7:533-554 pubmed publisher
    ..Notch activation in Defa4Cre-expressing cells caused dedifferentiation to multipotent ISCs in vivo and was required for adenoma formation. ADAM10 deletion had no significant effect on crypt homeostasis...
  23. Giordano N, Hastie J, Smith A, Foss E, Gutierrez Munoz D, Carlson P. Cysteine Desulfurase IscS2 Plays a Role in Oxygen Resistance in Clostridium difficile. Infect Immun. 2018;86: pubmed publisher
    ..These data imply an undefined role for this cysteine desulfurase in protecting C. difficile from low levels of oxygen in the gut. ..
  24. Chai S, Liu K, Feng W, Liu T, Wang Q, Zhou R, et al. Activation of G protein-coupled estrogen receptor protects intestine from ischemia/reperfusion injury in mice by protecting the crypt cell proliferation. Clin Sci (Lond). 2019;133:449-464 pubmed publisher
    ..can prompt epithelial cell repair following intestinal injury, which occurred at least in part by inhibiting the iNOS expression in intestinal stem cells (ISCs). GPER may be a novel therapeutic target for intestinal I/R injury.
  25. Moon Y, Kwon J, Yun S, Lim H, Kim J, Kim S, et al. Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1. Int J Mol Sci. 2015;16:9167-95 pubmed publisher
    ..The present results provide the first global atlas of proteome changes triggered by sulfur, and may facilitate an understanding of how hyperthermophilic archaea adapt to sulfur-rich, extreme environments. ..
  26. Wang W, Jeffery C. An analysis of surface proteomics results reveals novel candidates for intracellular/surface moonlighting proteins in bacteria. Mol Biosyst. 2016;12:1420-31 pubmed publisher
    ..The presence of intracellular proteins on the cell surface is more common than previously expected and suggests that many additional proteins might be candidates for being intracellular/surface moonlighting proteins. ..
  27. Cedeno R, Nakauka Ddamba A, Yousefi M, Sterling S, Leu N, Li N, et al. The histone variant macroH2A confers functional robustness to the intestinal stem cell compartment. PLoS ONE. 2017;12:e0185196 pubmed publisher
    ..is dispensable for intestinal homeostasis and macroH2A DKO mice have similar numbers of active crypt-base columnar ISCs (CBCs)...
  28. Meddings J, Saint S, Fowler K, Gaies E, Hickner A, Krein S, et al. The Ann Arbor Criteria for Appropriate Urinary Catheter Use in Hospitalized Medical Patients: Results Obtained by Using the RAND/UCLA Appropriateness Method. Ann Intern Med. 2015;162:S1-34 pubmed publisher
    ..The appropriateness of Foley catheters, intermittent straight catheters (ISCs), and external condom catheters for hospitalized adults on medical services was assessed in 299 scenarios, ..
  29. Teshima T, Reddy P, Zeiser R. Acute Graft-versus-Host Disease: Novel Biological Insights. Biol Blood Marrow Transplant. 2016;22:11-6 pubmed publisher
    ..Gastrointestinal (GI) GVHD is the major cause of mortality. Emerging data indicate that intestinal stem cells (ISCs) and their niche Paneth cells are targeted, resulting in dysregulation of the intestinal homeostasis and microbial ..
  30. Riemer P, Sreekumar A, Reinke S, Rad R, Schäfer R, Sers C, et al. Transgenic expression of oncogenic BRAF induces loss of stem cells in the mouse intestine, which is antagonized by β-catenin activity. Oncogene. 2015;34:3164-75 pubmed publisher
    ..Histological and gene expression analyses indicate that ISCs collectively converted to short-lived progenitor cells after BRAF activation...
  31. Mahé M, Sundaram N, Watson C, Shroyer N, Helmrath M. Establishment of human epithelial enteroids and colonoids from whole tissue and biopsy. J Vis Exp. 2015;: pubmed publisher
    ..This process is triggered by the proliferation of intestinal stem cells (ISCs) and progeny that progressively migrate and differentiate toward the tip of the villi...
  32. Teshima T, Reddy P, Zeiser R. Reprint of: Acute Graft-versus-Host Disease: Novel Biological Insights. Biol Blood Marrow Transplant. 2016;22:S3-8 pubmed publisher
    ..Gastrointestinal (GI) GVHD is the major cause of mortality. Emerging data indicate that intestinal stem cells (ISCs) and their niche Paneth cells are targeted, resulting in dysregulation of the intestinal homeostasis and microbial ..
  33. Koehler C, Perkins G, Ellisman M, Jones D. Pink1 and Parkin regulate Drosophila intestinal stem cell proliferation during stress and aging. J Cell Biol. 2017;216:2315-2327 pubmed publisher
    Intestinal stem cells (ISCs) maintain the midgut epithelium in Drosophila melanogaster Proper cellular turnover and tissue function rely on tightly regulated rates of ISC division and appropriate differentiation of daughter cells...
  34. Hartenstein V, Takashima S, Hartenstein P, Asanad S, Asanad K. bHLH proneural genes as cell fate determinants of entero-endocrine cells, an evolutionarily conserved lineage sharing a common root with sensory neurons. Dev Biol. 2017;431:36-47 pubmed publisher
    ..during early metamorphosis, undergo several rounds of mitosis that produce the endocrine cells and stem cells (ISCs) with which the fly is born...
  35. Gakh O, Ranatunga W, Galeano B, Smith D, Thompson J, Isaya G. Defining the Architecture of the Core Machinery for the Assembly of Fe-S Clusters in Human Mitochondria. Methods Enzymol. 2017;595:107-160 pubmed publisher
    ..are assembled from (i) elemental sulfur, provided by a pyridoxal phosphate-dependent cysteine desulfurase (human NFS1) and its stabilizing-binding partner (human ISD11), and (ii) elemental iron, provided by an iron-binding protein of ..
  36. Kim K, Maio N, Singh A, Rouault T. Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients. Hum Mol Genet. 2018;27:837-852 pubmed publisher
    ..the cytosolic Fe-S biogenesis pathway (ISC), including the primary scaffold, ISCU1, and the cysteine desulfurase, NFS1, and the CIA targeting complex, consisting of CIAO1, FAM96B and MMS19, to facilitate Fe-S cluster insertion into ..
  37. Saha P, Kumar S, Srivastava S, Sinha D, Pareek G, D Silva P. The presence of multiple cellular defects associated with a novel G50E iron-sulfur cluster scaffold protein (ISCU) mutation leads to development of mitochondrial myopathy. J Biol Chem. 2014;289:10359-77 pubmed publisher
    ..The biochemical results highlight that the G50E mutation results in compromised interaction with the sulfur donor NFS1 and the J-protein HSCB, thus impairing the rate of Fe-S cluster synthesis...
  38. Deng H, Gerencser A, Jasper H. Signal integration by Ca(2+) regulates intestinal stem-cell activity. Nature. 2015;528:212-7 pubmed publisher
    ..The metabotropic glutamate receptor (mGluR) is required in ISCs for this response, and for an associated modulation of cytosolic Ca(2+) oscillations that results in sustained high ..
  39. Janeckova L, Fafilek B, Krausova M, Horazna M, Vojtechova M, Alberich Jorda M, et al. Wnt Signaling Inhibition Deprives Small Intestinal Stem Cells of Clonogenic Capacity. Genesis. 2016;54:101-14 pubmed publisher
    ..Interestingly, suppression of Wnt/β-catenin signaling affected asymmetric division of intestinal stem cells (ISCs) rather than proliferation...
  40. Yousefi M, Li N, Nakauka Ddamba A, Wang S, Davidow K, Schoenberger J, et al. Msi RNA-binding proteins control reserve intestinal stem cell quiescence. J Cell Biol. 2016;215:401-413 pubmed
    ..homeostasis and a reserve, radioresistant Wntlow/off ISC capable of generating active Wnthigh ISCs. We examined the role of the Msi family of oncoproteins in the ISC compartment...
  41. Yin C, Xi R. A Phyllopod-Mediated Feedback Loop Promotes Intestinal Stem Cell Enteroendocrine Commitment in Drosophila. Stem Cell Reports. 2018;10:43-57 pubmed publisher
    The intestinal epithelium in the Drosophila midgut is maintained by intestinal stem cells (ISCs), which are capable of generating both enterocytes and enteroendocrine cells (EEs) via alternative cell fate specification...
  42. Zhang P, Liu Y, Wang M, Dong M, Liu Z, Jia Z, et al. Chinese mitten crab (Eriocheir sinensis) iron-sulphur cluster assembly protein 2 (EsIscA2) is differentially regulated after immune and oxidative stress challenges. Dev Comp Immunol. 2018;84:343-352 pubmed publisher
    Iron-sulphur clusters (ISCs), one of the oldest and most versatile cofactors of proteins, are involved in catalysis reactions, electron transport reactions, regulation processes as well as sensing of ambient conditions...
  43. Dai Y, Kim D, Dong G, Busenlehner L, Frantom P, Outten F. SufE D74R Substitution Alters Active Site Loop Dynamics To Further Enhance SufE Interaction with the SufS Cysteine Desulfurase. Biochemistry. 2015;54:4824-33 pubmed publisher
    ..These results show that dynamic changes may shift SufE to a sulfur-acceptor state that interacts more strongly with SufS. ..
  44. Yan R, Friemel M, Aloisi C, Huynen M, Taylor I, Leimkuhler S, et al. The Eukaryotic-Specific ISD11 Is a Complex-Orphan Protein with Ability to Bind the Prokaryotic IscS. PLoS ONE. 2016;11:e0157895 pubmed publisher
    ..coli co-purifies with the bacterial orthologue of NFS1, IscS. Binding is weak but specific suggesting that, despite the absence of Isd11 sequences in bacteria, there is enough ..
  45. Resende L, Truong M, Gomez A, Jones D. Intestinal stem cell ablation reveals differential requirements for survival in response to chemical challenge. Dev Biol. 2017;424:10-17 pubmed publisher
    The Drosophila intestine is maintained by multipotent intestinal stem cells (ISCs)...
  46. Nalapareddy K, Nattamai K, Kumar R, Karns R, Wikenheiser Brokamp K, Sampson L, et al. Canonical Wnt Signaling Ameliorates Aging of Intestinal Stem Cells. Cell Rep. 2017;18:2608-2621 pubmed publisher
    Although intestinal homeostasis is maintained by intestinal stem cells (ISCs), regeneration is impaired upon aging. Here, we first uncover changes in intestinal architecture, cell number, and cell composition upon aging...
  47. Jun S, Jung Y, Suh H, Wang W, Kim M, Oh Y, et al. LIG4 mediates Wnt signalling-induced radioresistance. Nat Commun. 2016;7:10994 pubmed publisher
    ..high Wnt signalling is associated with radioresistance in colorectal cancer (CRC) cells and intestinal stem cells (ISCs). We find that LIG4, a DNA ligase in DNA double-strand break repair, is a direct target of β-catenin...
  48. Franzetti E, Casartelli M, D Antona P, Montali A, Romanelli D, Cappellozza S, et al. Midgut epithelium in molting silkworm: A fine balance among cell growth, differentiation, and survival. Arthropod Struct Dev. 2016;45:368-79 pubmed publisher
    The midgut of insects has attracted great attention as a system for studying intestinal stem cells (ISCs) as well as cell death-related processes, such as apoptosis and autophagy...
  49. Čavužić M, Liu Y. Biosynthesis of Sulfur-Containing tRNA Modifications: A Comparison of Bacterial, Archaeal, and Eukaryotic Pathways. Biomolecules. 2017;7: pubmed publisher
    ..The detailed reaction mechanisms of Fe-S cluster depend s²U and s?U formation await further investigations. ..
  50. Ferecatu I, Canal F, Fabbri L, Mazure N, Bouton C, Golinelli Cohen M. Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1? activation. PLoS ONE. 2018;13:e0194782 pubmed publisher
    ..Interestingly, we show that hypoxia promotes the downregulation of several proteins (ISCU, NFS1, FXN) involved in the early steps of mitochondrial Fe-S cluster biogenesis...
  51. Li N, Nakauka Ddamba A, Tobias J, Jensen S, Lengner C. Mouse Label-Retaining Cells Are Molecularly and Functionally Distinct From Reserve Intestinal Stem Cells. Gastroenterology. 2016;151:298-310.e7 pubmed publisher
    ..injury are controlled by 2 different types of cells: slow cycling, injury-resistant reserve intestinal stem cells (ISCs) and actively proliferative ISCs...
  52. Tauc H, Tasdogan A, Meyer P, Pandur P. Nipped-A regulates intestinal stem cell proliferation in Drosophila. Development. 2017;144:612-623 pubmed publisher
    ..Various factors are known to govern ISC behavior; however, transcriptional changes in ISCs during aging are still unclear...
  53. Lucchetta E, Ohlstein B. Amitosis of Polyploid Cells Regenerates Functional Stem Cells in the Drosophila Intestine. Cell Stem Cell. 2017;20:609-620.e6 pubmed publisher
    ..Here, we show that after severe depletion, intestinal stem cells (ISCs) in the Drosophila midgut are replaced by spindle-independent ploidy reduction of cells in the enterocyte lineage ..
  54. Ma M, Zhao H, Zhao H, Binari R, Perrimon N, Li Z. Wildtype adult stem cells, unlike tumor cells, are resistant to cellular damages in Drosophila. Dev Biol. 2016;411:207-216 pubmed publisher
    ..We find that in the adult Drosophila midgut, intestinal stem cells (ISCs), unlike tumor intestinal cells, are resistant to various cellular damages...
  55. Xu C, Luo J, He L, Montell C, Perrimon N. Oxidative stress induces stem cell proliferation via TRPA1/RyR-mediated Ca2+ signaling in the Drosophila midgut. elife. 2017;6: pubmed publisher
    ..Our findings provide a link between redox regulation and Ca2+ signaling and reveal a novel mechanism by which ISCs detect stress signals.
  56. Dutta D, Dobson A, Houtz P, Gläßer C, Revah J, Korzelius J, et al. Regional Cell-Specific Transcriptome Mapping Reveals Regulatory Complexity in the Adult Drosophila Midgut. Cell Rep. 2015;12:346-58 pubmed publisher
    ..five morphologically and functionally distinct regions (R1-R5), each composed of multipotent intestinal stem cells (ISCs), progenitor enteroblasts (EBs), enteroendocrine cells (EEs), enterocytes (ECs), and visceral muscle (VM)...
  57. Pain D, Dancis A. Roles of Fe-S proteins: from cofactor synthesis to iron homeostasis to protein synthesis. Curr Opin Genet Dev. 2016;38:45-51 pubmed publisher
    ..These defects can then result in cancer, neurologic dysfunction or type 2 diabetes. ..
  58. Zhang S, Han X, Wei B, Fang J, Wei H. RSPO2 enriches LGR5(+) spheroid colon cancer stem cells and promotes its metastasis by epithelial-mesenchymal transition. Am J Transl Res. 2016;8:354-64 pubmed
    ..RSPO2, the agonist of canonical Wnt/beta-catenin pathway and serves as the growth factor of intestinal stem cells (ISCs), is considered playing an important role in CCSCs...
  59. Chen J, Cai H, Suo L, Xue Y, Wang J, Wan Q. A systematic review of the survival and complication rates of inlay-retained fixed dental prostheses. J Dent. 2017;59:2-10 pubmed publisher
    ..05). Compared with conventional fixed dental prostheses (FDPs) and implant-supported single crowns (ISCs), IRFDPs exhibited an acceptable 3-year survival rate but higher complication rates of debonding and veneer ..
  60. Curatti L, Rubio L. Challenges to develop nitrogen-fixing cereals by direct nif-gene transfer. Plant Sci. 2014;225:130-7 pubmed publisher
    ..Expression of active NifH requires the products of nifM, nifH, and possibly nifU and nifS, whereas active NifDK requires the products of nifH, nifD, nifK, nifB, nifE, nifN, and possibly nifU, nifS, nifQ, ..
  61. Antonello Z, Reiff T, Ballesta Illan E, Dominguez M. Robust intestinal homeostasis relies on cellular plasticity in enteroblasts mediated by miR-8-Escargot switch. EMBO J. 2015;34:2025-41 pubmed publisher
    ..We found that intestinal stem cells (ISCs) divide "ahead" of demand during Drosophila midgut homeostasis...
  62. Takashima S, Aghajanian P, Younossi Hartenstein A, Hartenstein V. Origin and dynamic lineage characteristics of the developing Drosophila midgut stem cells. Dev Biol. 2016;416:347-60 pubmed publisher
    Proliferating intestinal stem cells (ISCs) generate all cell types of the Drosophila midgut, including enterocytes, endocrine cells, and gland cells (e.g., copper cells), throughout the lifetime of the animal...
  63. Kim R, Sheaffer K, Choi I, Won K, Kaestner K. Epigenetic regulation of intestinal stem cells by Tet1-mediated DNA hydroxymethylation. Genes Dev. 2016;30:2433-2442 pubmed
    ..Here, we generated genome-wide maps of the 5hmC mark in ISCs and their differentiated progeny...
  64. Fischer J, Calabrese P, Miller A, Munoz N, Grady W, Shibata D, et al. Single cell lineage tracing reveals a role for Tgf?R2 in intestinal stem cell dynamics and differentiation. Proc Natl Acad Sci U S A. 2016;113:12192-12197 pubmed
    Intestinal stem cells (ISCs) are maintained by a niche mechanism, in which multiple ISCs undergo differential fates where a single ISC clone ultimately occupies the niche...
  65. Zou W, Blutt S, Zeng X, Chen M, Lo Y, Castillo Azofeifa D, et al. Epithelial WNT Ligands Are Essential Drivers of Intestinal Stem Cell Activation. Cell Rep. 2018;22:1003-1015 pubmed publisher
    Intestinal stem cells (ISCs) maintain and repair the intestinal epithelium...
  66. Baechler B, McKnight C, Pruchnicki P, Biro N, Reed B. Hindsight/RREB-1 functions in both the specification and differentiation of stem cells in the adult midgut of Drosophila. Biol Open. 2015;5:1-10 pubmed publisher
    ..Four fundamental cell types are found in the adult midgut epithelium: undifferentiated intestinal stem cells (ISCs) and their committed daughter cells, enteroblasts (EBs), plus enterocytes (ECs) and enteroendocrine cells (EEs)...
  67. Tian A, Benchabane H, Wang Z, Ahmed Y. Regulation of Stem Cell Proliferation and Cell Fate Specification by Wingless/Wnt Signaling Gradients Enriched at Adult Intestinal Compartment Boundaries. PLoS Genet. 2016;12:e1005822 pubmed publisher
    ..and proliferation are directed by Wnt/β-catenin signaling in mammals, whereas aberrant Wnt pathway activation in ISCs triggers the development of human colorectal carcinoma...
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    ..Males succumb to intestinal challenges to which females are resistant, associated with fewer proliferating ISCs, suggesting a trade-off between highly active repair mechanisms and late-life pathology in females...
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    ..Overexpression of dJmj in intestinal stem cells/enteroblasts (ISCs/EBs) induces cell-autonomous ISC proliferation followed by differentiation, that is controlled by the Notch and ..
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    ..Three global regulator (IscS, TusA and NarJ) and four chaperone proteins (DsbA, DsbB, NifS and XdhC) were overexpressed to aid the formation and ordered assembly of three redox center cofactors of ..
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    ..In human mitochondria, a core Fe-S assembly complex [called SDUF and composed of NFS1, ISD11, ISCU2, and frataxin (FXN) proteins] synthesizes Fe-S clusters from iron, cysteine sulfur, and reducing ..
  72. Gong W, Guo M, Han Z, Wang Y, Yang P, Xu C, et al. Mesenchymal stem cells stimulate intestinal stem cells to repair radiation-induced intestinal injury. Cell Death Dis. 2016;7:e2387 pubmed publisher
    ..In particular, Lgr5+ intestinal stem cells (ISCs) are indispensable for intestinal regeneration following exposure to radiation...
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    ..Mammalian intestinal stem cells (ISCs) are located in bases of crypts but at least two groups of cells have been cited as stem cells...
  74. Aghaallaei N, Gruhl F, Schaefer C, Wernet T, Weinhardt V, Centanin L, et al. Identification, visualization and clonal analysis of intestinal stem cells in fish. Development. 2016;143:3470-3480 pubmed
    ..model of symmetrical stem cell division followed by neutral drift has been proposed for intestinal stem cells (ISCs), which has been suggested to represent the predominant mode of stem cell progression in mammals...
  75. Friemel M, Marelja Z, Li K, Leimkuhler S. The N-Terminus of Iron-Sulfur Cluster Assembly Factor ISD11 Is Crucial for Subcellular Targeting and Interaction with l-Cysteine Desulfurase NFS1. Biochemistry. 2017;56:1797-1808 pubmed publisher
    ..The initial sulfur mobilization step for FeS cluster biosynthesis is catalyzed by l-cysteine desulfurase NFS1, a reaction that is localized in mitochondria in humans...
  76. Bühning M, Valleriani A, Leimkuhler S. The Role of SufS Is Restricted to Fe-S Cluster Biosynthesis in Escherichia coli. Biochemistry. 2017;56:1987-2000 pubmed publisher
    ..The IscS protein of this system was additionally revealed to be the primary sulfur donor for several sulfur-containing ..
  77. Takagi T, Yoshimura S, Sakuma R, Nakano Doi A, Matsuyama T, Nakagomi T. Novel Regenerative Therapies Based on Regionally Induced Multipotent Stem Cells in Post-Stroke Brains: Their Origin, Characterization, and Perspective. Transl Stroke Res. 2017;8:515-528 pubmed publisher
    ..they differentiate into more than neural lineages, we now refer to them as ischemia-induced multipotent stem cells (iSCs). Very recently, we showed that putative iNSPCs/iSCs are present within post-stroke areas in human brains...
  78. Bühning M, Friemel M, Leimkuhler S. Functional Complementation Studies Reveal Different Interaction Partners of Escherichia coli IscS and Human NFS1. Biochemistry. 2017;56:4592-4605 pubmed publisher
    ..The human l-cysteine desulfurase NFS1 and the Escherichia coli homologue IscS share a level of amino acid sequence identity of ?60%. While E...
  79. Wang Q, Liu J, Li H, Yang S, Körmöczi P, Kereszt A, et al. Nodule-Specific Cysteine-Rich Peptides Negatively Regulate Nitrogen-Fixing Symbiosis in a Strain-Specific Manner in Medicago truncatula. Mol Plant Microbe Interact. 2017;: pubmed publisher
    ..meliloti A145 is controlled by multiple genes in M. truncatula, including NFS1 and NFS2 that encode nodule-specific cysteine-rich (NCR) peptides...
  80. Lee C, Minich A, Li B, Miyake H, Seo S, Pierro A. Influence of stress factors on intestinal epithelial injury and regeneration. Pediatr Surg Int. 2018;34:155-160 pubmed publisher
    Lgr5+ intestinal epithelial stem cells (ISCs) crucial for intestinal epithelial regeneration are impaired during necrotizing enterocolitis...
  81. Anjum M, Green J, Guest J. YeiL, the third member of the CRP-FNR family in Escherichia coli. Microbiology. 2000;146 Pt 12:3157-70 pubmed
    ..and sulphide contents increased consistent with the acquisition of one [4Fe-4S] cluster per monomer after anaerobic NifS-catalysed reconstitution...
  82. Marelja Z, Mullick Chowdhury M, Dosche C, Hille C, Baumann O, Löhmannsröben H, et al. The L-cysteine desulfurase NFS1 is localized in the cytosol where it provides the sulfur for molybdenum cofactor biosynthesis in humans. PLoS ONE. 2013;8:e60869 pubmed publisher
    In humans, the L-cysteine desulfurase NFS1 plays a crucial role in the mitochondrial iron-sulfur cluster biosynthesis and in the thiomodification of mitochondrial and cytosolic tRNAs...
  83. Pandey A, Gordon D, Pain J, Stemmler T, Dancis A, Pain D. Frataxin directly stimulates mitochondrial cysteine desulfurase by exposing substrate-binding sites, and a mutant Fe-S cluster scaffold protein with frataxin-bypassing ability acts similarly. J Biol Chem. 2013;288:36773-86 pubmed publisher
    ..in mitochondria, the sulfur is derived from the amino acid cysteine by the cysteine desulfurase activity of Nfs1. The enzyme binds the substrate cysteine in the pyridoxal phosphate-containing site, and a persulfide is formed on ..
  84. Lu R, VOIGT R, Zhang Y, Kato I, Xia Y, Forsyth C, et al. Alcohol Injury Damages Intestinal Stem Cells. Alcohol Clin Exp Res. 2017;41:727-734 pubmed publisher
    ..The renewal and functional regulation of the intestinal epithelium largely relies on intestinal stem cells (ISCs)...
  85. Rocha A, Knight S, Pandey A, Yoon H, Pain J, Pain D, et al. Cysteine desulfurase is regulated by phosphorylation of Nfs1 in yeast mitochondria. Mitochondrion. 2018;40:29-41 pubmed publisher
    The cysteine desulfurase Nfs1/Isd11 uses the amino acid cysteine as the substrate and its activity is absolutely required for contributing persulfide sulfur to the essential process of iron-sulfur (Fe-S) cluster assembly in mitochondria...
  86. Boniecki M, Freibert S, Muhlenhoff U, Lill R, Cygler M. Structure and functional dynamics of the mitochondrial Fe/S cluster synthesis complex. Nat Commun. 2017;8:1287 pubmed publisher
    ..De novo Fe/S cluster synthesis occurs on the mitochondrial scaffold protein ISCU and requires cysteine desulfurase NFS1, ferredoxin, frataxin, and the small factors ISD11 and ACP (acyl carrier protein)...
  87. Suh H, Kim M, Jung Y, Lien E, Jun S, Park J. Quiescence Exit of Tert+ Stem Cells by Wnt/?-Catenin Is Indispensable for Intestinal Regeneration. Cell Rep. 2017;21:2571-2584 pubmed publisher
    ..However, the mechanism of quiescence exit of Tert+ intestinal stem cells (ISCs) remains unknown...
  88. Siudeja K, Nassari S, Gervais L, Skorski P, Lameiras S, Stolfa D, et al. Frequent Somatic Mutation in Adult Intestinal Stem Cells Drives Neoplasia and Genetic Mosaicism during Aging. Cell Stem Cell. 2015;17:663-74 pubmed publisher
    ..Here, we report two mechanisms of genome instability in adult Drosophila intestinal stem cells (ISCs) that cause phenotypic alterations in the aging intestine...
  89. Gakh O, Ranatunga W, Smith D, Ahlgren E, Al Karadaghi S, Thompson J, et al. Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery. J Biol Chem. 2016;291:21296-21321 pubmed
    ..following four proteins involved in the initial step of Fe-S cluster synthesis: FXN42-210 (iron donor); [NFS1]·[ISD11] (sulfur donor); and ISCU (scaffold upon which new clusters are assembled)...
  90. Angerer H, Schönborn S, Gorka J, Bahr U, Karas M, Wittig I, et al. Acyl modification and binding of mitochondrial ACP to multiprotein complexes. Biochim Biophys Acta Mol Cell Res. 2017;1864:1913-1920 pubmed publisher
    ..complex I bound fraction, ACPM1 is present as a free matrix protein and in complex with the soluble LYRM4(ISD11)/NFS1 complex implicated in Fe-S cluster biogenesis...
  91. Sousa Victor P, Ayyaz A, Hayashi R, Qi Y, Madden D, Lunyak V, et al. Piwi Is Required to Limit Exhaustion of Aging Somatic Stem Cells. Cell Rep. 2017;20:2527-2537 pubmed publisher
    ..Here, we show that intestinal stem cells (ISCs) induce the Argonaute family protein Piwi in response to JAK/STAT signaling during acute proliferative episodes...
  92. Morgenstern Y, Das Adhikari U, Ayyash M, Elyada E, Tóth B, Moor A, et al. Casein kinase 1-epsilon or 1-delta required for Wnt-mediated intestinal stem cell maintenance. EMBO J. 2017;36:3046-3061 pubmed publisher
    The intestinal epithelium holds an immense regenerative capacity mobilized by intestinal stem cells (ISCs), much of it supported by Wnt pathway activation...